LOCUS       DANJLF010000001       120400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000001 DANJLF010000000
VERSION     DANJLF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 120400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 120400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..120400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            33..914
                     /locus_tag="PWT25_000001"
     CDS             33..914
                     /locus_tag="PWT25_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020121.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            927..2537
                     /locus_tag="PWT25_000002"
     CDS             927..2537
                     /locus_tag="PWT25_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL1020122.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2614..3273)
                     /locus_tag="PWT25_000003"
     CDS             complement(2614..3273)
                     /locus_tag="PWT25_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL1020123.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3556..4221)
                     /locus_tag="PWT25_000004"
     CDS             complement(3556..4221)
                     /locus_tag="PWT25_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL1020124.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4368..4973)
                     /gene="dhaL"
                     /locus_tag="PWT25_000005"
     CDS             complement(4368..4973)
                     /gene="dhaL"
                     /locus_tag="PWT25_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL1020125.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5001..6011)
                     /gene="dhaK"
                     /locus_tag="PWT25_000006"
     CDS             complement(5001..6011)
                     /gene="dhaK"
                     /locus_tag="PWT25_000006"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL1020126.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5986..6432)
                     /gene="dhaM"
                     /locus_tag="PWT25_000007"
     CDS             complement(5986..6432)
                     /gene="dhaM"
                     /locus_tag="PWT25_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL1020127.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6535..6855
                     /locus_tag="PWT25_000008"
     CDS             6535..6855
                     /locus_tag="PWT25_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL1020128.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6967..7836)
                     /locus_tag="PWT25_000009"
     CDS             complement(6967..7836)
                     /locus_tag="PWT25_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1020129.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7932..9235)
                     /locus_tag="PWT25_000010"
                     /pseudo
     CDS             complement(7932..9235)
                     /locus_tag="PWT25_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9232..9741)
                     /locus_tag="PWT25_000011"
     CDS             complement(9232..9741)
                     /locus_tag="PWT25_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020130.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9916..12051)
                     /locus_tag="PWT25_000012"
     CDS             complement(9916..12051)
                     /locus_tag="PWT25_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1020131.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12134..13789)
                     /locus_tag="PWT25_000013"
     CDS             complement(12134..13789)
                     /locus_tag="PWT25_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1020132.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13791..14105)
                     /locus_tag="PWT25_000014"
     CDS             complement(13791..14105)
                     /locus_tag="PWT25_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL1020133.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14275..14535
                     /locus_tag="PWT25_000015"
     CDS             14275..14535
                     /locus_tag="PWT25_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL1020134.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14549..14863
                     /locus_tag="PWT25_000016"
     CDS             14549..14863
                     /locus_tag="PWT25_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL1020135.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15593..16147)
                     /locus_tag="PWT25_000017"
     CDS             complement(15593..16147)
                     /locus_tag="PWT25_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL1020136.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16273..18201)
                     /locus_tag="PWT25_000018"
     CDS             complement(16273..18201)
                     /locus_tag="PWT25_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL1020137.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18507..19223)
                     /locus_tag="PWT25_000019"
     CDS             complement(18507..19223)
                     /locus_tag="PWT25_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL1020138.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19224..21047)
                     /gene="glpO"
                     /locus_tag="PWT25_000020"
     CDS             complement(19224..21047)
                     /gene="glpO"
                     /locus_tag="PWT25_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL1020139.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21061..22557)
                     /gene="glpK"
                     /locus_tag="PWT25_000021"
     CDS             complement(21061..22557)
                     /gene="glpK"
                     /locus_tag="PWT25_000021"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL1020140.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22693..24153)
                     /locus_tag="PWT25_000022"
     CDS             complement(22693..24153)
                     /locus_tag="PWT25_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020141.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24143..25438)
                     /locus_tag="PWT25_000023"
     CDS             complement(24143..25438)
                     /locus_tag="PWT25_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1020142.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25770..26447)
                     /locus_tag="PWT25_000024"
     CDS             complement(25770..26447)
                     /locus_tag="PWT25_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL1020143.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26477..27568)
                     /locus_tag="PWT25_000025"
     CDS             complement(26477..27568)
                     /locus_tag="PWT25_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL1020144.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27669..28103)
                     /locus_tag="PWT25_000026"
     CDS             complement(27669..28103)
                     /locus_tag="PWT25_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1020145.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28687..30144)
                     /gene="ascB"
                     /locus_tag="PWT25_000027"
     CDS             complement(28687..30144)
                     /gene="ascB"
                     /locus_tag="PWT25_000027"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL1020146.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30141..32036)
                     /locus_tag="PWT25_000028"
     CDS             complement(30141..32036)
                     /locus_tag="PWT25_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1020147.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32094..33869)
                     /locus_tag="PWT25_000029"
     CDS             complement(32094..33869)
                     /locus_tag="PWT25_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL1020148.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33919..35415)
                     /locus_tag="PWT25_000030"
     CDS             complement(33919..35415)
                     /locus_tag="PWT25_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1020149.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35609..37231)
                     /locus_tag="PWT25_000031"
     CDS             complement(35609..37231)
                     /locus_tag="PWT25_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL1020150.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37680..38993)
                     /locus_tag="PWT25_000032"
     CDS             complement(37680..38993)
                     /locus_tag="PWT25_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL1020151.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39238..39462)
                     /locus_tag="PWT25_000033"
     CDS             complement(39238..39462)
                     /locus_tag="PWT25_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020152.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39482..39904)
                     /locus_tag="PWT25_000034"
     CDS             complement(39482..39904)
                     /locus_tag="PWT25_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL1020153.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40048..40758)
                     /locus_tag="PWT25_000035"
     CDS             complement(40048..40758)
                     /locus_tag="PWT25_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020154.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40776..41273)
                     /locus_tag="PWT25_000036"
     CDS             complement(40776..41273)
                     /locus_tag="PWT25_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1020155.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41310..41762)
                     /locus_tag="PWT25_000037"
     CDS             complement(41310..41762)
                     /locus_tag="PWT25_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1020156.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41897..42502)
                     /locus_tag="PWT25_000038"
     CDS             complement(41897..42502)
                     /locus_tag="PWT25_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL1020157.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42952..43572)
                     /locus_tag="PWT25_000039"
     CDS             complement(42952..43572)
                     /locus_tag="PWT25_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1020158.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43574..44443)
                     /locus_tag="PWT25_000040"
     CDS             complement(43574..44443)
                     /locus_tag="PWT25_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL1020159.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44466..44873)
                     /locus_tag="PWT25_000041"
     CDS             complement(44466..44873)
                     /locus_tag="PWT25_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL1020160.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44892..46181)
                     /locus_tag="PWT25_000042"
     CDS             complement(44892..46181)
                     /locus_tag="PWT25_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1020161.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46208..46510)
                     /locus_tag="PWT25_000043"
     CDS             complement(46208..46510)
                     /locus_tag="PWT25_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1020162.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46535..48628)
                     /locus_tag="PWT25_000044"
     CDS             complement(46535..48628)
                     /locus_tag="PWT25_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1020163.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49117..49881)
                     /locus_tag="PWT25_000045"
     CDS             complement(49117..49881)
                     /locus_tag="PWT25_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL1020164.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49992..50861)
                     /gene="mdcB"
                     /locus_tag="PWT25_000046"
     CDS             complement(49992..50861)
                     /gene="mdcB"
                     /locus_tag="PWT25_000046"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL1020165.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50886..51962)
                     /locus_tag="PWT25_000047"
     CDS             complement(50886..51962)
                     /locus_tag="PWT25_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL1020166.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51986..52579)
                     /locus_tag="PWT25_000048"
     CDS             complement(51986..52579)
                     /locus_tag="PWT25_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL1020167.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52583..54259)
                     /gene="mdcD"
                     /locus_tag="PWT25_000049"
     CDS             complement(52583..54259)
                     /gene="mdcD"
                     /locus_tag="PWT25_000049"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL1020168.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54249..54548)
                     /locus_tag="PWT25_000050"
     CDS             complement(54249..54548)
                     /locus_tag="PWT25_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL1020169.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54650..56296)
                     /gene="mdcA"
                     /locus_tag="PWT25_000051"
     CDS             complement(54650..56296)
                     /gene="mdcA"
                     /locus_tag="PWT25_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL1020170.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56303..57214)
                     /locus_tag="PWT25_000052"
     CDS             complement(56303..57214)
                     /locus_tag="PWT25_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL1020171.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57334..58191
                     /locus_tag="PWT25_000053"
     CDS             57334..58191
                     /locus_tag="PWT25_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1020172.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58378..58740)
                     /locus_tag="PWT25_000054"
     CDS             complement(58378..58740)
                     /locus_tag="PWT25_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL1020173.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            59086..60525
                     /locus_tag="PWT25_000055"
     CDS             59086..60525
                     /locus_tag="PWT25_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020174.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60532..63084)
                     /locus_tag="PWT25_000056"
     CDS             complement(60532..63084)
                     /locus_tag="PWT25_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL1020175.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63198..64529)
                     /locus_tag="PWT25_000057"
     CDS             complement(63198..64529)
                     /locus_tag="PWT25_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1020176.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64495..65973)
                     /locus_tag="PWT25_000058"
     CDS             complement(64495..65973)
                     /locus_tag="PWT25_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1020177.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66278..68113)
                     /gene="tdc"
                     /locus_tag="PWT25_000059"
     CDS             complement(66278..68113)
                     /gene="tdc"
                     /locus_tag="PWT25_000059"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL1020178.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            68583..70010
                     /locus_tag="PWT25_000060"
     CDS             68583..70010
                     /locus_tag="PWT25_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020179.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(70102..70467)
                     /locus_tag="PWT25_000061"
     CDS             complement(70102..70467)
                     /locus_tag="PWT25_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL1020180.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(70464..70829)
                     /locus_tag="PWT25_000062"
     CDS             complement(70464..70829)
                     /locus_tag="PWT25_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL1020181.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(71021..71269)
                     /locus_tag="PWT25_000063"
     CDS             complement(71021..71269)
                     /locus_tag="PWT25_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020182.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            71268..71396
                     /locus_tag="PWT25_000064"
     CDS             71268..71396
                     /locus_tag="PWT25_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL1020183.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            71453..73147
                     /locus_tag="PWT25_000065"
     CDS             71453..73147
                     /locus_tag="PWT25_000065"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL1020184.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            73151..73270
                     /locus_tag="PWT25_000066"
                     /pseudo
     CDS             73151..73270
                     /locus_tag="PWT25_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            73247..73420
                     /locus_tag="PWT25_000067"
     CDS             73247..73420
                     /locus_tag="PWT25_000067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020185.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            73798..74931
                     /locus_tag="PWT25_000068"
     CDS             73798..74931
                     /locus_tag="PWT25_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL1020186.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            74962..75282
                     /locus_tag="PWT25_000069"
     CDS             74962..75282
                     /locus_tag="PWT25_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL1020187.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(75311..75775)
                     /locus_tag="PWT25_000070"
     CDS             complement(75311..75775)
                     /locus_tag="PWT25_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1020188.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(75907..77160)
                     /locus_tag="PWT25_000071"
     CDS             complement(75907..77160)
                     /locus_tag="PWT25_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1020189.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(77189..77506)
                     /locus_tag="PWT25_000072"
     CDS             complement(77189..77506)
                     /locus_tag="PWT25_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL1020190.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(77532..77852)
                     /locus_tag="PWT25_000073"
     CDS             complement(77532..77852)
                     /locus_tag="PWT25_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1020191.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(78316..79731)
                     /locus_tag="PWT25_000074"
     CDS             complement(78316..79731)
                     /locus_tag="PWT25_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1020192.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(79748..81064)
                     /locus_tag="PWT25_000075"
     CDS             complement(79748..81064)
                     /locus_tag="PWT25_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1020193.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(81214..81912)
                     /locus_tag="PWT25_000076"
     CDS             complement(81214..81912)
                     /locus_tag="PWT25_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL1020194.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(82201..83157)
                     /locus_tag="PWT25_000077"
     CDS             complement(82201..83157)
                     /locus_tag="PWT25_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1020195.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(83270..84103)
                     /gene="msrA"
                     /locus_tag="PWT25_000078"
     CDS             complement(83270..84103)
                     /gene="msrA"
                     /locus_tag="PWT25_000078"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL1020196.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(84278..85912)
                     /locus_tag="PWT25_000079"
     CDS             complement(84278..85912)
                     /locus_tag="PWT25_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020197.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(85899..87767)
                     /locus_tag="PWT25_000080"
     CDS             complement(85899..87767)
                     /locus_tag="PWT25_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020198.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            87733..89370
                     /locus_tag="PWT25_000081"
     CDS             87733..89370
                     /locus_tag="PWT25_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020199.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(89515..90255)
                     /gene="truA"
                     /locus_tag="PWT25_000082"
     CDS             complement(89515..90255)
                     /gene="truA"
                     /locus_tag="PWT25_000082"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL1020200.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(90446..91081)
                     /locus_tag="PWT25_000083"
     CDS             complement(90446..91081)
                     /locus_tag="PWT25_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL1020201.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(91228..92469)
                     /gene="sstT"
                     /locus_tag="PWT25_000084"
     CDS             complement(91228..92469)
                     /gene="sstT"
                     /locus_tag="PWT25_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL1020202.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            92667..93665
                     /locus_tag="PWT25_000085"
     CDS             92667..93665
                     /locus_tag="PWT25_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020203.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            93678..95696
                     /locus_tag="PWT25_000086"
     CDS             93678..95696
                     /locus_tag="PWT25_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL1020204.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(95756..96472)
                     /locus_tag="PWT25_000087"
     CDS             complement(95756..96472)
                     /locus_tag="PWT25_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL1020205.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(96699..97241)
                     /locus_tag="PWT25_000088"
     CDS             complement(96699..97241)
                     /locus_tag="PWT25_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL1020206.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(97246..97473)
                     /locus_tag="PWT25_000089"
     CDS             complement(97246..97473)
                     /locus_tag="PWT25_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL1020207.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(97552..99432)
                     /locus_tag="PWT25_000090"
     CDS             complement(97552..99432)
                     /locus_tag="PWT25_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL1020208.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(99621..100235)
                     /locus_tag="PWT25_000091"
     CDS             complement(99621..100235)
                     /locus_tag="PWT25_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1020209.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            100377..100937
                     /locus_tag="PWT25_000092"
     CDS             100377..100937
                     /locus_tag="PWT25_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1020210.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(101242..101556)
                     /locus_tag="PWT25_000093"
                     /pseudo
     CDS             complement(101242..101556)
                     /locus_tag="PWT25_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(101810..103445)
                     /locus_tag="PWT25_000094"
                     /pseudo
     CDS             complement(101810..103445)
                     /locus_tag="PWT25_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(104000..104563)
                     /locus_tag="PWT25_000095"
     CDS             complement(104000..104563)
                     /locus_tag="PWT25_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1020211.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(104578..105621)
                     /locus_tag="PWT25_000096"
     CDS             complement(104578..105621)
                     /locus_tag="PWT25_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020212.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(105754..106158)
                     /locus_tag="PWT25_000097"
     CDS             complement(105754..106158)
                     /locus_tag="PWT25_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL1020213.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(106391..107026)
                     /locus_tag="PWT25_000098"
     CDS             complement(106391..107026)
                     /locus_tag="PWT25_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL1020214.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(107292..108344)
                     /locus_tag="PWT25_000099"
     CDS             complement(107292..108344)
                     /locus_tag="PWT25_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1020215.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(108357..109295)
                     /locus_tag="PWT25_000100"
     CDS             complement(108357..109295)
                     /locus_tag="PWT25_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL1020216.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(109746..110396)
                     /locus_tag="PWT25_000101"
     CDS             complement(109746..110396)
                     /locus_tag="PWT25_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020217.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(110496..111005)
                     /locus_tag="PWT25_000102"
     CDS             complement(110496..111005)
                     /locus_tag="PWT25_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDL1020218.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(111206..112075)
                     /locus_tag="PWT25_000103"
     CDS             complement(111206..112075)
                     /locus_tag="PWT25_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1020219.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(112095..113216)
                     /locus_tag="PWT25_000104"
     CDS             complement(112095..113216)
                     /locus_tag="PWT25_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL1020220.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(113218..113613)
                     /locus_tag="PWT25_000105"
     CDS             complement(113218..113613)
                     /locus_tag="PWT25_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL1020221.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(113632..114438)
                     /locus_tag="PWT25_000106"
     CDS             complement(113632..114438)
                     /locus_tag="PWT25_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1020222.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(114431..115219)
                     /locus_tag="PWT25_000107"
     CDS             complement(114431..115219)
                     /locus_tag="PWT25_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1020223.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(115233..115709)
                     /locus_tag="PWT25_000108"
     CDS             complement(115233..115709)
                     /locus_tag="PWT25_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1020224.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(115781..118438)
                     /locus_tag="PWT25_000109"
     CDS             complement(115781..118438)
                     /locus_tag="PWT25_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1020225.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(118457..118861)
                     /locus_tag="PWT25_000110"
     CDS             complement(118457..118861)
                     /locus_tag="PWT25_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDL1020226.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(119093..119350)
                     /locus_tag="PWT25_000111"
     CDS             complement(119093..119350)
                     /locus_tag="PWT25_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL1020227.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(119475..119807)
                     /locus_tag="PWT25_000112"
     CDS             complement(119475..119807)
                     /locus_tag="PWT25_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020228.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        35880 a        24841 c        19858 g        39821 t
ORIGIN      
        1 tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt
       61 tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag
      121 gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac
      181 ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg
      241 aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg
      301 gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg
      361 ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa
      421 actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg
      481 atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc
      541 aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc
      601 tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga acagtcgtag
      661 aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag
      721 aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc
      781 acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca
      841 aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac
      901 attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt
      961 tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg
     1021 ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg
     1081 gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt
     1141 cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca
     1201 gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag
     1261 aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag
     1321 ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga
     1381 tgattttttt ccaagcagat tcgatcactg cagaaggtgc ttggcttttt gggctatctg
     1441 taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa
     1501 gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag
     1561 caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt
     1621 tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc
     1681 taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac accggctata
     1741 tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat
     1801 tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg
     1861 cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga
     1921 aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg
     1981 tcgtcttgat ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag
     2041 aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt
     2101 attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg
     2161 gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga
     2221 tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg
     2281 gctttctatt cggatggctt cctcgaatgg gtaaacttgc ttatatctac ttagcttact
     2341 cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag
     2401 ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta
     2461 cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag
     2521 acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt
     2581 caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc
     2641 aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa
     2701 ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat
     2761 tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac
     2821 tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt
     2881 ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga
     2941 gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga
     3001 ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg
     3061 taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac
     3121 gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg
     3181 atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg
     3241 aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta aacagctatt
     3301 tctaataaag aaacataact tcccttattc caactgataa ttttctttta tcatagcata
     3361 aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat
     3421 tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt
     3481 tgaataatca aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg
     3541 cttatttcgt gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag
     3601 ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa
     3661 tagtttaccc atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt
     3721 tgtatcggac aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg
     3781 ccatatgtaa taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa
     3841 ctggttatac gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa
     3901 atagatttgg tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg
     3961 agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct
     4021 ttcgatttta ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg
     4081 aatctctttc catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc
     4141 tgttggatgg ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata
     4201 atctgatttt ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga
     4261 attttgcgat atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa
     4321 cagccaaaca aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg
     4381 atcacatcta aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt
     4441 ccgattgcac gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg
     4501 tgttcttttg cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg
     4561 actggcagcc aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga
     4621 atacccgctg ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg
     4681 acctcttttg tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc
     4741 ccaccgactt tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca
     4801 ggatgatcgg cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca
     4861 tggtctccat cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt
     4921 gcctctgcaa attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca
     4981 cccttttatc ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct
     5041 ttccattgtg tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag
     5101 gtcataaaat taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct
     5161 aaaacatcat tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact
     5221 agtacacctg cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc
     5281 tttgccagta ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata
     5341 ccaacaccat actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt
     5401 acagttgctg ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc
     5461 agttctttca actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt
     5521 acaaggacag tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg
     5581 atatcatcat ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt
     5641 gccatatcga agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt
     5701 ccactatccg cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca
     5761 tcaccaagca ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca
     5821 tgtccgcttc ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca
     5881 acacgggaag attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc
     5941 atttcttcga caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt
     6001 ctttccggca ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt
     6061 gatggctgct gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg
     6121 ctgctcttct tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc
     6181 cgcaaaaaca aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat
     6241 cgggtctgat ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga
     6301 tttttgcatc tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat
     6361 aagcaaaata ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg
     6421 tttcgctttc attaatgaac aattcaagta tataataaat ttctaatttt gggaagatat
     6481 gtgtagtgct tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt
     6541 acaacaacaa cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt
     6601 ggtgaggtca ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc
     6661 tttggcggcc gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt
     6721 gccgaaatgg ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg
     6781 gactatgaag tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct
     6841 gtctccacga gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta
     6901 gtggcactca agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc
     6961 tcttttttat ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc
     7021 tgctaaggaa taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt
     7081 tgatttggat ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga
     7141 tttttctcct gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc
     7201 tttttttgct tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc
     7261 ttgactggcc gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc
     7321 aacctgattt aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt
     7381 ttggacagct tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc
     7441 atttcccact ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt
     7501 caatttgttt tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc
     7561 ttgcaaatga tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga
     7621 tgaaatcgtt tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc
     7681 accgttttca gcaccagaag agcttatagt tgttgaaggt tctgtttgag caggttcaga
     7741 agtttccgca aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat
     7801 taacggcttc attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc
     7861 ttattataag caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa
     7921 cggtgcatct tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct
     7981 aaaatcgttt tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag
     8041 aacaaaagca tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct
     8101 acaatcagcg aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca
     8161 aaactgaatg taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg
     8221 attaatccag caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat
     8281 gtttgatcaa tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt
     8341 ttgaaattcg tcagccagac ttctgttcca ccttttgccc aacgttttct ttgtcgatat
     8401 agcatcttca acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat
     8461 aaccagtcat ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt
     8521 ctgaatccgc ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac
     8581 gcataaagac caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag
     8641 aattcaaccg tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca
     8701 gtgaccgcag caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt
     8761 gcatctggct ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag
     8821 gcaagtccga tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga
     8881 agattagcgt attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca
     8941 tcgtctgtca ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt
     9001 gtatcttcga tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg
     9061 acactcaagg gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac
     9121 cagacaccaa taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaataca
     9181 taatagacaa acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag
     9241 ttccacccca ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc
     9301 ggagacagaa taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata
     9361 cacttcttca agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt
     9421 atttactttt ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag
     9481 gaaactgaaa aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga
     9541 taaaaaagcg tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac
     9601 ccatataaaa ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat
     9661 ctttaaccag taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc
     9721 ttctcttgtt acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt
     9781 accatacatt tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac
     9841 aaaacatttc tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta
     9901 ttttttatct cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga
     9961 tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata
    10021 taagattaca tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt
    10081 ttctaatcca tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt
    10141 ttgggtgata actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt
    10201 caacaattct tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat
    10261 cgatacgatg tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc
    10321 tccgctagaa cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc
    10381 gaaacaacgc ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc
    10441 acctactaaa gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag
    10501 ggtataatct tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc
    10561 aacaagtaaa tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg
    10621 ccaagcataa gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc
    10681 cacccatgcc atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt
    10741 ccttgcataa gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc
    10801 caactgccta tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg
    10861 tttcaaaaga tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc
    10921 gatcaattta ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag
    10981 gactgttttt ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg
    11041 atctgattgt tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga
    11101 tacattttct cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga
    11161 aagctgtgaa accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg
    11221 taggacattc gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc
    11281 ggatgtcatc gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga
    11341 ggttttacca cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat
    11401 gattgtgttt tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc
    11461 ttggcctaag acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt
    11521 gaccaaacga tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt
    11581 attttgacga accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat
    11641 tggtgaacgc caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc
    11701 gatataaaag gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta
    11761 gggacttttc aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat
    11821 attcatctga tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc
    11881 taagtaactg tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc
    11941 ttctattttt gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg
    12001 atacacgttc ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct
    12061 cctctttttt taaagtggtg gaaccgatta gtttacctct tttaagagtc gaatgcaagc
    12121 tttttaacga gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata
    12181 aagaaaatgc accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg
    12241 ctagatagct acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt
    12301 catttaatcg gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct
    12361 tttccaattc ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg
    12421 gcaattcatg ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc
    12481 ctagcatgtt tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt
    12541 ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt
    12601 tatccacacc ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga
    12661 agatcaactt cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg
    12721 atacatgaat gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg
    12781 tggatgcggc agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc
    12841 ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag
    12901 caagagaaat aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca
    12961 ccgcataaat atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta
    13021 tgcctaattc gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata
    13081 aaataactaa atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt
    13141 cttcaaatcc agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt
    13201 tttctataaa agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga
    13261 tcaccatgac ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc
    13321 ctgcaccaac gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga
    13381 tatctggtac atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg
    13441 gaacaactgg ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt
    13501 gaccctcatg tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat
    13561 ttggacgcac atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat
    13621 tttcccgttc actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa
    13681 catacggtcc tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg
    13741 tagcagcaga cattccacct gctacacctc cgacaataac gattttcatc ttatttccct
    13801 cctactgttc gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca
    13861 gatttcttta attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag
    13921 acggtctttt ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct
    13981 ccttttatat gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt
    14041 tccaaaagct tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa
    14101 ttcattttct gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga
    14161 gctttattaa tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc
    14221 ataaaatacc gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag
    14281 attgataata aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc
    14341 caaaaaatga tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt
    14401 cgttctagta ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt
    14461 gagaaacctg atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg
    14521 attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga
    14581 aaaatggtca gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac
    14641 gattgtcgta tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga
    14701 attcttagat ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca
    14761 tatttcagaa agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt
    14821 agttgaactg caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac
    14881 agaagtgatc agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac
    14941 cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca gccaaacaaa
    15001 aatagcttct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
    15061 accgtttaag gaagaggttg tgaaatcagc ggtttgacct aagtattaga gaaaaaaacg
    15121 ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
    15181 acaccgttta aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt
    15241 tgtgaaaatg gatttctaca tttttgcata aatttggctt aataccgaga aactggcttt
    15301 tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagaaac
    15361 aaacggaaaa atagctgctc atattttgcc gttttttgag ctaataccga aggtcagctg
    15421 attgaacacc gtttaccaag aggttgtgag cctaccattt aactccgaat cacaagaaat
    15481 aatttcaaaa acaacttctc ctgtttttgg tttggttggc ttattatcga gaagtagcta
    15541 ctgggagtct atttatcaaa aaaaggaatg cgacataggt cacacccctt ttttattttc
    15601 tttgaaacat ctttcggtac tcgacagcat acaagacaaa agattgtaaa agaccgtaag
    15661 tgatgacggc aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa agcagcaaaa
    15721 ggatcatgcc aacatagatg acagcagtcg ctactttgcc ataccacttc gctccactta
    15781 atttgatatt tttagatagc ccgattaatc cagctacaaa cataaagcct tcttttatca
    15841 agaatagacc aaacaacagc caaagccacg gtcgatacca tgccatagac aaaatcagaa
    15901 aaagttgtgt caatttatct gcaaacggat caagcacctt ccctaagtct gttacctgat
    15961 tatatctccg agcaatgacc ccgtctagat aatcagacaa acctgagaca acaatgaccg
    16021 ctcctgctat ataaaatgac tgtctcagca caaaataaag ataaatcggc agcaaaaaaa
    16081 tacggaatat cgataaaaga ttcggaatcg tttgtatttc tttacgccag tctgtgctta
    16141 atttcatttt atcgccctac ctcctcagca ctcttttctt tttattttag cttacacggg
    16201 gaataaaacc aatgatttca ctgtttttat ttttctttac agccactgat attatgagct
    16261 aatttgagat aatcacttgc tgttttcatc gtttactctt ctttcttcct gtcgtttgcg
    16321 catttctttt actagtactt tatgagcttt ttttgatcct cgctctaggt cacgctgccg
    16381 tttacgtgtt ttataatcga taaacagact gtctaaatcg taacctgtcg ggtacagatc
    16441 ttctgctgaa cgcaccagac gaacacgctg acgaggcacg tggattattt ttttgtctaa
    16501 atatacatca atcgtatctt cacctatatc ttcataaact aacgcttctt tttggtattc
    16561 attgacaaat atccgatcac cttttgcaaa gagctggcgt tcttttttat tctccttcct
    16621 atttgtaact gctgtcttag gaagaaattc ttttttctgt gtccgatagg ctttttccgc
    16681 caagtaacgc tctgcttgat ggatcaattt catcgacata tccattttat gagcaatcca
    16741 caaagcttgg ctttctcctg tttctccaac ccgcaactga tatttaggac gaagtgcttc
    16801 ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga atttcttgat
    16861 ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag attccatgat
    16921 tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct catctaaaag
    16981 taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca tatgaccaga
    17041 aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga aaatctcatc
    17101 aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca tcgccatcaa
    17161 agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg ctccggtgat
    17221 aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat gctcaggtaa
    17281 aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc taggtgtgct
    17341 tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca ccgtgatcgt
    17401 ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca atgccgccag
    17461 gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt cattcaattt
    17521 agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat cgataatcgt
    17581 tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt cgcctttttg
    17641 aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga atttcagtaa
    17701 ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac gcaaattacg
    17761 tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact cttctacatt
    17821 tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt attgattctt
    17881 atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt cgatcaaatc
    17941 ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga tcctcggcaa
    18001 ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc ttgtttcttc
    18061 ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga ttcgtttttt
    18121 gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa attgtaattg
    18181 actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg tcccaaaata
    18241 aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc atgtcctatc
    18301 cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag cacaccaaat
    18361 aaactttgtt tattggctat tcgacttact gattagatac cagctacact cacaaaacat
    18421 accccattct tctaggtctt atttattttc cacttaacta tagcaagaca cattttggtt
    18481 gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc taccgcgata
    18541 attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc tgaagttcct
    18601 ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg agcagggttg
    18661 attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa tccacctaca
    18721 gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa cccaagaact
    18781 aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg tatagcagga
    18841 cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca atgtggcaaa
    18901 taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc aatgatgaaa
    18961 ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga gactgcagga
    19021 ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac agcgagtccc
    19081 caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt taaattcaca
    19141 gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa ctctcccatg
    19201 atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag atctgattct
    19261 gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt ccattcaaaa
    19321 tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc tcgttcaaac
    19381 aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat ttcttcttct
    19441 aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg tgccatttct
    19501 tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat ataagtagca
    19561 tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac tgtttcttct
    19621 aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc gatttctttc
    19681 gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc tccttctgcc
    19741 atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc tggttctctt
    19801 tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct gttttccaaa
    19861 tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata ttcacttact
    19921 gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc accattgtaa
    19981 tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc ttcgatatca
    20041 ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag ataatctaca
    20101 tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc tgtcgtacca
    20161 aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg ctttcctgta
    20221 tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa atggatccct
    20281 ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg tactttatct
    20341 acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac ttcatctgtc
    20401 agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa gccaaccgct
    20461 ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt atcaatcact
    20521 aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag cccttctttt
    20581 ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt ctcatatttg
    20641 gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac cgagaacata
    20701 ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc tggttttggg
    20761 atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc tactacttcc
    20821 acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga gcgagaggaa
    20881 gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga tgctgctgac
    20941 tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc caattcttct
    21001 tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg gatttcctcc
    21061 ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa gtttttctcg
    21121 gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt cgcggatctc
    21181 gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc ctagcgccgt
    21241 tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt ggaattgcat
    21301 cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct tgattccagt
    21361 gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact gtaaagtagc
    21421 tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc ctcgtgctgc
    21481 ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa cctcatcttc
    21541 gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca tttgaagtcc
    21601 atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta aagcatagta
    21661 aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg ataattgcgg
    21721 tttttcaccg gtattcatga caataaatga acctgtacca taagtattct tgaccatgcc
    21781 tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca tccctgaaat
    21841 tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt ctgagttaga
    21901 agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct cctgatccca
    21961 atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat cagtaacatg
    22021 tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa acatcaattc
    22081 gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc gtatcttcgt
    22141 tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt cttgatagcc
    22201 ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa caactgcatg
    22261 atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct ggttcgttat
    22321 gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc cggcaataac
    22381 tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt cttgcgggaa
    22441 atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt caaagataat
    22501 tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt ctgtcattgt
    22561 gattccctcc attatatgca aacgctttct ttaactactc ttatcatact actgttttaa
    22621 aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt ttattatgaa
    22681 gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt tcaatcgaat
    22741 aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt tgtttctctg
    22801 gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa tctcaatacc
    22861 gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat aaatcggcag
    22921 actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga tcatcaaaag
    22981 aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc ttttcctcgg
    23041 caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg aatgcccgag
    23101 cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct ttccaacaaa
    23161 gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt cttctttgat
    23221 tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag tatctaaaac
    23281 agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt cttcgtctac
    23341 tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat tcagttctgc
    23401 tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa gccggtccga
    23461 ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat tcaatagtct
    23521 ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg taaacgttgt
    23581 ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat tttgcaaaaa
    23641 aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat atcgtatctg
    23701 tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct ctaagagctg
    23761 gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg ctgtcgctaa
    23821 tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa acttcaagct
    23881 ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca acacaagcag
    23941 cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc caatcaattc
    24001 aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct ctctcaaacc
    24061 attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga tctgtaactg
    24121 ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc tttgaccaca
    24181 ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct aactgttcaa
    24241 acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac atctcttttt
    24301 tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat ttgagtttaa
    24361 ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca attttttggg
    24421 gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag ccaaaccact
    24481 gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta attgtcttgc
    24541 tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt ttttcatttg
    24601 ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt tgagacgttt
    24661 gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt tcttcccaca
    24721 tctgattgtc ggggcgagaa tgattagcca gcaaaacaag atcatagttc tcctttcttt
    24781 tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt tcttctaaaa
    24841 aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt tcaggatcta
    24901 gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt tttcttgatg
    24961 ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca attttctttg
    25021 cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact gggtgaataa
    25081 gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct agaatcaaac
    25141 gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc aatcctatta
    25201 ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct ttcgtgatcc
    25261 aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac ccgctgggaa
    25321 tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc atcaattggc
    25381 gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca atgttcatct
    25441 gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa acacagtatt
    25501 cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc ctctccatca
    25561 taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga agagtatcat
    25621 ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg ccttttacgt
    25681 acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg tgtgtaatgt
    25741 aatagttaga atagacacat catttatttt taattcttta atgcttcaac aactgcttcg
    25801 cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc aatttgaacc
    25861 acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa agcatcttct
    25921 gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt actgaaccca
    25981 gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg caacaatcct
    26041 acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg tgaagcgact
    26101 ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc agcataataa
    26161 accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc caaggcccga
    26221 tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt caaaacactt
    26281 tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc gatcccattt
    26341 tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt aggacgctcg
    26401 atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt cccatcctct
    26461 ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat gggcttggca
    26521 aataaagctc tttatatgct tcttcaaaag gtttcgttac aaattcatcc tcacggtttt
    26581 ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt gctgcaagtt
    26641 ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg acaccaacta
    26701 aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta tcgttgtatt
    26761 tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa tctagttttg
    26821 actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct ggtgtttcat
    26881 attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat tgttcaaccg
    26941 ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc aagctaccgc
    27001 cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc aaatacgtat
    27061 ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt gtggctttgc
    27121 caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt gtttccgcat
    27181 caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat aaagttgctc
    27241 gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct tctccttgga
    27301 ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg ctgagttcac
    27361 ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca gcaccaagac
    27421 ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca ttgaaaaagc
    27481 tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact cctgaaggta
    27541 aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc tctgattaac
    27601 ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac taatttgctt
    27661 aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg ggctaattct
    27721 ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc ccagctacca
    27781 tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga caagcgacct
    27841 tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg agcaatcgtt
    27901 gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc tttttctttt
    27961 aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa cgcttcatca
    28021 gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca ttcatctaac
    28081 gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc atagtatgat
    28141 ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag atttgaaaaa
    28201 aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct tctggaacac
    28261 cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa attttgtgaa
    28321 aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg cttttgaaca
    28381 ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga acagccaaac
    28441 aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca gctgattgta
    28501 caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag gagcaatttc
    28561 aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag ttggcaggag
    28621 aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt cccttatttc
    28681 cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat caaatgattt
    28741 tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat agatatatcc
    28801 ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc cccacgacgt
    28861 ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa tatgctgttt
    28921 caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt tgtctatagc
    28981 tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata attgattcaa
    29041 agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg gaagatacgg
    29101 atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt ctggatgagc
    29161 actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt aggctgctaa
    29221 gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa acattcgttg
    29281 cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag aagttctttg
    29341 ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa gaagctgata
    29401 gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt ttgtagctaa
    29461 agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca aatggggatg
    29521 gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt tgatctcatt
    29581 gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt cagcgaaacg
    29641 aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa ccaaatataa
    29701 cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta gacattcgtc
    29761 aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat caccatgagg
    29821 aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt cagcaaataa
    29881 acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag gataataata
    29941 cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat gccattgatc
    30001 tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca cagataatcc
    30061 tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc cgccccataa
    30121 aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt tcttgtttga
    30181 ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt aacaataacc
    30241 attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc catcaataaa
    30301 tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt gccatttaaa
    30361 ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact gactacccca
    30421 attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacaggact gtagattttt
    30481 ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa tgttggatcg
    30541 tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac tatatccttt
    30601 gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat acaggaactg
    30661 atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat ataaatcggg
    30721 aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc accagctact
    30781 ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata tttaagagtc
    30841 acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc agataaagat
    30901 aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc tcctgcttgc
    30961 gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa agaaagattg
    31021 ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac ttgccaaaat
    31081 ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa atatccagct
    31141 gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaagt aattgggacc
    31201 atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg caagcttttg
    31261 cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg aataaccgta
    31321 cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat gctatcacta
    31381 tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc gatgaaacga
    31441 tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa atagaaaaat
    31501 gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa taaattcaaa
    31561 ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac gcctaaagtc
    31621 ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt tttctcagtt
    31681 cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa tacgtcatac
    31741 acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc gactacagtg
    31801 atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc ttttagggta
    31861 aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc tccgacaaat
    31921 cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt gttaattaat
    31981 gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt ctccattcaa
    32041 ttagtacccc ttataatagc gaacgaaact gtacaatagt gtgtatggtt tacttagccg
    32101 agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata gtcaaaggag
    32161 acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc aagctgatac
    32221 tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt ctcggcattt
    32281 aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga atctatcttt
    32341 ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt tgttttcttt
    32401 ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca agattcaaac
    32461 tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag ctcttgctca
    32521 gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa aagatacact
    32581 ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg tttttttagt
    32641 gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg ttttcgaaaa
    32701 ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa attgggacgt
    32761 aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg ataacgggag
    32821 atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct cgttttcatt
    32881 tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac aggaagatct
    32941 aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc tttgtttttt
    33001 tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc ttgtttttct
    33061 tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt ctctaccatc
    33121 ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg aagaatggtc
    33181 aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata attatatgat
    33241 ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa aattgtactg
    33301 atttgttgag gcaaccttgc gtgttggatc accgtttctg ttccaacaag agataagaaa
    33361 attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga aacttccagt
    33421 tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa tgatacggct
    33481 gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc tttttgaaac
    33541 aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat tttatgtttc
    33601 gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa aaaataacct
    33661 gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat atttttgatt
    33721 tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc gcttgttatc
    33781 caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt taagtccatt
    33841 tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg acaatagtca
    33901 cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt taccattaga
    33961 ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa agcttccgct
    34021 cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt cccctgttcc
    34081 tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc aaccatcttt
    34141 tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt ttggatcgtg
    34201 gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa ctatgaataa
    34261 tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta gaggatcaat
    34321 tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga aaccatttgc
    34381 tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac gataataact
    34441 aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat ctaacattac
    34501 tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat gccctcggca
    34561 catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa atacattctc
    34621 tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa tacccatctt
    34681 agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta gaaacatatg
    34741 atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa gtttatctct
    34801 agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat tattgatctc
    34861 attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg tctgcgcata
    34921 aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc ctaccaatgc
    34981 taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc gatttaatgt
    35041 attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt cgtctcctct
    35101 tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta actccttcag
    35161 taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag aaagataatg
    35221 gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact gaatactagg
    35281 atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc cgtcttccaa
    35341 ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat tttctggaaa
    35401 tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt gaagtatagc
    35461 tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa aaaattgaat
    35521 gctactcttc atttttaata tttatatata acaaatacat aagacgatcc ccgaaagaaa
    35581 acactcgtta accatctaag caaacgattt attttacttc caacacaaat gcttcataag
    35641 gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac acaataaccc
    35701 ttaaagcttc acgtggaaca gtgaacgtct gcttttcact ggacatatta caaataacga
    35761 gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc gtcttttcca
    35821 ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg atcaatttct
    35881 tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg atttctttat
    35941 aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt tcacgggcgt
    36001 tccattgcat aggcgtccta gcattgtctc ttcctttcgc attgattgat tccaagattt
    36061 cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc tcaatatctt
    36121 ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct ccttgataga
    36181 tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca ctttcttttc
    36241 gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga ttattccaaa
    36301 ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat acttttttca
    36361 gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc tgatctaatc
    36421 ctatgtgttc gaattggaag accatggata attcatttct tttaggattg gaataaagtt
    36481 tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt ttgtcaccaa
    36541 aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta cttgtgattt
    36601 ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc ccaatacctt
    36661 tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta tttttccaat
    36721 tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt ttttcatcca
    36781 actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt ccattgaccg
    36841 gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg gcctcaatga
    36901 accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata cggatatttc
    36961 ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat tctgccatga
    37021 ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg tatactggag
    37081 aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa ataatccccc
    37141 gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag atttgataaa
    37201 caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg gatgtttcta
    37261 ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc tttcaaatag
    37321 ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg aaattccctc
    37381 aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac aacttcttta
    37441 taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa aatatgagga
    37501 actatttttt cgtttttttc tctaatactc aggccagaac actgatttca caacctcttt
    37561 ataaacggtg ttcactcagc tgtccctcgg tattagctca aaaaacggca aaatataaga
    37621 aactattttt tcgttttttt ctctaatact caggccagaa cgctgatttc acaacctctt
    37681 tatttcacat tcaatatcca catgaatccc acaaatacaa agaacagacc atacatgatt
    37741 ggagatactt cttttcctct tttagctgct aacatggtaa ttggatagaa gataaaacct
    37801 aatgcaatcc catctgaaat actataagtc aaaggcatgc cgatcaagat caagaatgaa
    37861 gggatcgcaa tctctaagtt tccccattcg atttgtcgca aagattgtgc catcaatact
    37921 cctaccataa tcaatgctgg tgcagttact tgtgaagtaa caactgatag taatggagaa
    37981 aagaacaagc ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc tgaacgtcct
    38041 ccaacggcga taccagctga tgattctaca aatgcaccaa caggagaagt accaaataag
    38101 gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt tccctacacg aggcatcttg
    38161 ttgtctttca tgaaacctgc ttggtttgct aatccaacta aagttcctgc tgtatcaaag
    38221 aaagtcacaa gcaagaatgt cagcactacg acccacattt gaagagaatt gatgtcccct
    38281 acatgtttta atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc aacaatttca
    38341 gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc tatcccgatg
    38401 aagatgccac ctggtacacg ccgaacaagc aagatagcag ttacgactaa tccaaaaatc
    38461 gtcaaccaag tagaaccaac gttgagtgat cctaatccta ctaatgtaga ttcattcgaa
    38521 acgatgatcc caccttcgct cagaccaagg aaagcgataa atagaccgat accgccagaa
    38581 atcgcatatt tcaaatctgc tgggatcgca tcaatgatca tttcccgcag cttgaagatt
    38641 gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt ttcccatgga
    38701 atccccattc ctaaacaaac agaataagca aaaaaagcat tgatcccaag agcaggtgcc
    38761 gttccaattg gataacgggc taataccccc attaaaacac agccgatcgc actggctaat
    38821 gcagtcgcag taaacactgc tccttcatcc attccagaag cacctaatac agttggattt
    38881 acaaataaga tataagccat tgagataaat gttgtaaatc ctgccagtac ctcacgtttt
    38941 accgtggtgt tcaatttttc tatttcgaag taggaaagta ttttttcctt catcttccat
    39001 ccccctaaat ccgaacatta taaataaagt ccgttaaaat ttcgatttat taataaaaca
    39061 acgtgtttta tcatatacta aaacaaaaca taagtaaaca aaaaattcgg attttaagca
    39121 aaatccgaac gttccatctg attaaatccc tttatctcaa agttcatgaa cattcacttt
    39181 ggattacaag aatttcttta ttgtgttaat aaagtttctt cattctagtc tcaaaaatca
    39241 atagtgtgat ttgattttac catttcgttt ctgttcttct ttgatgaatt cttttgtatc
    39301 gacaggattc agattattta gtccgccaaa acctgtatcg accatttttc cccagccttt
    39361 tgtcttcttt tttcctgatt tgagattctt tgctcgaagc ttctcactat gttttgctcg
    39421 ccgatacttc aaataacctt ctaatcttcc cattagatcc actgtttttc ctccttttta
    39481 ttcaatcaga cgactcagct tttcctataa caaaataatt gccttcaggg tcagaaaaat
    39541 tgaaagtcaa gatgccattg ttatctgtga tttctcctgg ttcttccatg ttctcgtaga
    39601 tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg tggtcctaat acctctggtg
    39661 aaaacttctg cacaaatact tttgggaata cgcctaattc taattctttt gagatgctga
    39721 gtacaatact cttaaattct tctggcaatt cgattgtttc agaaatttct gcaccaattt
    39781 tttctacaaa gaatcggcaa atcatctcta cgtcattgac ataaagcatc acacgtgttt
    39841 tttgtatcat catttgaccc ttctttctat ttgattttct ttcctgtcta taaactgatt
    39901 ccattatatc acgaaagcgc aaacaccttg tgtttgatac tattcaagaa aaataaagca
    39961 atagtttcta ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa aacggcaatg
    40021 aatgccgctc ttcttatttc gattctttta taattctgct tctgattcat ctttggttag
    40081 tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt ttttccttca acgcttcgat
    40141 tgcctgtgcg ccacgtgccg cttgttcagc attgatggat tcaatgacca gcaccgctgc
    40201 ttgtgtttct tccgtcagca ttgttctttg ggcttcacgc cagttgagac agcgacagat
    40261 cgctccttgt tcatcatagt agatgatttc ttccggaagt gccggagcat cggaatctgc
    40321 acctaaaggc aggaatgatt cgccaccttg ggcttttcct aaatgcagat ttcctgcgat
    40381 cttttcgata tcttctccgc cgcaaggtac gccaaaagac aaggaaatac tattatagat
    40441 atctacaagc ggattgattg ggaaaaattc ccgttcttga tttgcacgtt tcagtaaagc
    40501 ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct gacgccactg
    40561 agcaatgact tcgttttggc tgaatgtttc ttctgtaagg taatttcttg cttcttttgc
    40621 tgcctcattc aataattgat gatacgtttc ttcactttct tttgttacgt ggttatcgat
    40681 accatttacg gataagattt cgatggttgc ttcaggaaat acttcccaga aattggtatc
    40741 cactattact tttttcatct gttcacacct cttgattaga ataattgctt gatgaataga
    40801 tagtctgttt ggggatcatc tcccatataa aaaacatgtt cgctgtattt gataaatcct
    40861 tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag ccaaatggct
    40921 gttttttgct tttcttttgc tttttcaata gctgtatcta gtaaaaatgt accgatacct
    40981 tgatgtttaa atttttttcg tacatagatt cgctcgattt ctaaaaaatg atcagccatt
    41041 tcctcagatt gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc ttgatcgata
    41101 ctcactagga aaaaatcggt ttctttatct tttagctctt ctgtaagttt ttctagtgca
    41161 taagcttgtg tcaagtaatc agctaaatgc tccggtgaat tatcttttcc aaatgtatct
    41221 gtaaatgttt cgatactaat ttgttgtagt aagtctacat ctgaaggcgt catttttcga
    41281 atttcgtatt tattcacggc ttcttccttt caatagatcc ttttattgcc ttttttgaca
    41341 aaattccaat cttcgctaac attcttgcat actttattta aaagatattc caattgtttt
    41401 gcttcttcgt cagaaagacc atttaatgcg actgcattgg agtagttgtt ttctcgaatg
    41461 atgaaaggat agatttctgc tcctttttta gtcggataga gtttcttgat ttttttattt
    41521 tctttatctt ccaaacgttc gatcatccca ttggattcta atttcttgat tgctcgggca
    41581 gtcgttgtac gatctacttt gatcatttct gctagttttt cttggatgat tccaggattt
    41641 tcgcaaatac gtactaaata aagatactgt cctcttgtta aatcgacttc tttgaattcg
    41701 atattgctaa tagaatctag cgctcgggca atcattccga tgtctcgaag gatttcagcc
    41761 atttcatccc acctttattt tgtttgtaag actagtttac ctctttttgt tgcaaatgca
    41821 actattttca ataaaaaaat caagttcttt tttaataact agtaaagtat attcttttct
    41881 tcttttacga aaaagctcaa tcacaataga tctggttttt gttttctaaa ataagtaact
    41941 gtttcttcgt tgttcgcttt tctgatagct ttggcaactc ttctatacta aaaaatccca
    42001 tgttggaagt ttcattattc tcaataaact tggcctcttc accgtggatc gcacaagcaa
    42061 aaacaagttt ataatactga aaaagctgtg gaatatcttt ttgtttattc gtatcaaaaa
    42121 ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc ttcatagact tctttacgca
    42181 cattttcttc aggagacaat cctatttcag caaatccacc aggaagtgac cattctttcg
    42241 tccgtttatc ttcgattagt aggaccttgc catctttttt gatataggca cgaacatcta
    42301 cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc ttttatttct tcgaaagttt
    42361 cttttcctat ttcactgatc agttccaatg aaatcgtccg gatctcctca aaacgctctt
    42421 tatcaaatac atcttttcca taatatagac cagcttgaga aagtgcgagt agtcttttgt
    42481 attgagccaa cttcttgttc attctcttcc cttctttttt agcaaatgta tgactatgta
    42541 atcaagtata gaagactggt ttaattacgg caaataaata taatgaaatc tgcaataaat
    42601 aaaagaatgt taaaaaactc gagaacaaaa gacattcgac ttctctgggg ctgtgaataa
    42661 atggcgttca aaagcttgca tctgcggtaa taagccccac caaacgaaaa atatgacgag
    42721 ctattcttgt ctggctgttc ttattaattg aggcaggaca gctttttcac aacctcccca
    42781 ctaaacggtg ttctcctacc aactcctcgg tcacaagtca caatattcat caaacatgag
    42841 aagctgtttt tttgaaattg ctccttgtga ctcggagctg aacgacaggc tcacgacctc
    42901 tcggtaaaca gcgttcaaaa gccaattttt cgatattaag ccatttcttt gttacttatt
    42961 gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt gagtatagtc
    43021 tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc ggtcggcgat
    43081 gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat cttccaatac
    43141 gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt cacgatcacg
    43201 tttgtaccga cgttgacata acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg
    43261 atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac taaaaggata
    43321 ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa
    43381 aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa acccgatgga
    43441 ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg gaaatcgttc
    43501 ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca gaagaatgaa
    43561 atactttttc atctcattta tttcctaagt taattggact ttccaagttc ataaacagtc
    43621 caatcttatg cttgacaatt tcttttactg cttcatttgc tggaatcaca ttatggcgaa
    43681 gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc cagttgacta
    43741 aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca ggataatctt
    43801 cttctttcac acctgttcct ccatggataa ctagggggac atcaactact gcatcgattt
    43861 ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga ttcgtaccga
    43921 tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg tcttccggtc
    43981 ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg ccgattgttc
    44041 caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact gcttttgtct
    44101 tcaggatatt ttctttgaaa ggcagatggg acccatcata catgacagaa gtatatccgc
    44161 tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc aagactgcat
    44221 caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc atgccgatat
    44281 atttagcggt atcgacactt gtttgaataa tgatcggcga acccatttct tttgctgcgc
    44341 ctagcatagc aggaagcatt tctaggttat gcgtattaaa agctccaact gtaaaattca
    44401 actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat tgccacctta
    44461 tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat ctcatccatt
    44521 ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc tgcacgcttc
    44581 tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt attttccaaa
    44641 ctcttttcta aatattggat ggcttgatcg ttcacataca attcagaaaa tttaagtcca
    44701 acttgttcat aaagtttcgc taatttatgc ttatcttggg tagtggaaat ctcttgtttc
    44761 aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc tttttcttct
    44821 tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa catcattttc
    44881 cctactttca attaaaatgc tttcaaaact gggcttaaga tccaggcata taagagtgct
    44941 gcggctactg tatctacatc ggttgctgta gcattgatga atcccatcga attaaagatc
    45001 gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc gccaatgatc
    45061 gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa
    45121 ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt aaacatggca
    45181 ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg gctataagga
    45241 aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat agctatccct
    45301 cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa tacatacaca
    45361 cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg atctcctgat
    45421 aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag cgggaccatc
    45481 acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg caacttgata
    45541 tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac accggcttca
    45601 aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc agtgatttta
    45661 cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc gataaaacca
    45721 ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc
    45781 gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga
    45841 gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc tctacctaaa
    45901 cccaaatcat tcattgcttg accattgata gattcaatag aagggatgat cccttgcata
    45961 tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat gatgctcgaa
    46021 ccggcactca acacttgaaa tcctaggagt gtctttaacg tgccagagat gacttgtcct
    46081 gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt gatcgatgcc
    46141 tgagtcaaaa tattttcgat gataaaattg ataacgccca tgtttttacc ctcttttctt
    46201 tttgtattta gataactccc ttatcttgca gaactggggt gattttttct tcaatttcat
    46261 tttttgaaac aatatttttc aaatagatca tcgccgtttt aggatcaact gcgaactttt
    46321 cgaactgcga acgaaaattt tctgctgtga tgatcacatc tggtttcatg gaaactgcgg
    46381 aagaaatatc acaatggtcc agtttggctt ctactcctaa tttgttcaaa acatcctcag
    46441 cagacatctg tgctgcaaaa ctacttccta aacctgcacc gcatacgaat aaaatgttca
    46501 atttgttcat ctttgttctc ccccgtatca tttattattt ctcaactact gaaaaaagca
    46561 gttgtttgaa atcttcgata tttgtgcatt caatcaatcg atccaacttc tcttcgttgt
    46621 tgatcaattc aattagttct ttcatgatgt tcagatgcga gtaggagtct actgctgcca
    46681 aacaaaaaat aatctttact ggatccattt caggattacc gaaatcgatt ggttgctcaa
    46741 tcgtagctac gctgactcct aatttattga ccccatcttc tggtcgggca tgggctagtg
    46801 ccatgtgttt gccaatcacg atatacggtc cgtaatcatt cactgcacga atcatcgctt
    46861 cgacatagcg ctcttcgaca atgttttctt taaccaatgg tctagcagct agttcaatcg
    46921 attcctccca agtagtggct ttttctttga tcatgatatt gctgtccgtc aaaatatctt
    46981 ttaacatagg ctggatctcc cttttgttga tttctaaatt gtttgtttcg aataattttt
    47041 ctaacttgcc atagatgatt cgatctactc ggccaccact ttctttcatg acatctatta
    47101 tttgagtgaa taactccgtc gattcatgac tgttaggctc taaccgctgt agttcttcat
    47161 ttcttttcaa aaagtcttct ataaccaaga tattcgtctc agttaaaatc ggatcgacaa
    47221 ctaaaagcgg cttatttcga taattcaatt gaaaggtgct gaaaaccaga ttgacatcca
    47281 atttatctac taattcgatt tcttttgaac ttaaaactgc agtaatctcg atctgaggaa
    47341 acttttcttt cagattctct gctaacagat ttccagtagc catcccatgg ttacagacaa
    47401 cgactgattt ataaacataa ttcaagtctc gcttgattac actggcgatc gtcgaaaagt
    47461 ggatgacaag aaaagctatc tcgtcctcca gtatcttgcc accaattact tcttcgatcg
    47521 taggaacaaa atgagtgact gcgcggtaga ttgggaaata atttgttcgg atgttttctt
    47581 tcaatggatt catgacttgg atatgattct tgattcttac aatcagtgca gccatgtgct
    47641 tatagagatt ttcgaataaa gcttcttcct ttagatggaa aggtacgtgt gtctcgttct
    47701 ctacaaattg gatcaattgg atcgttagta attgagcttg tacccactcg attgaattgg
    47761 agatatctct agcattgaac gtctcaatag tgaaacgaag ataatttctt tctacttgcg
    47821 ggacattgtc caattgaaat tcctcgatca tgtggtcaat aaatgcagaa aagctactgt
    47881 cttttatttc tgtatttttc caattaatgg cagagataaa ttcatgtttc ctgattcgct
    47941 ggatccagat caatgtaaaa agcaggattt gttttttata aatatcatct tcttgtttca
    48001 acaacttatt ggaatagatc tcctgtattt ttttgatttc caccaaagga aaataatgga
    48061 gaaagatttt ttgagccggt gttaattgtg catcttgatt cataaagaag tcggtcattc
    48121 ccagattctg attgataaaa tcatagatca tcgtacggat agatcgttct gaacctttat
    48181 aaaccagtcc ttgctttcca taactgacga tctctatccc ataactgacc agtgttgctc
    48241 gtaaccgtct catatcttca tctaatgtac ttttagagat ttgaaattct tcttcttttt
    48301 tgtttagatg gatcgggatt cggctgtaag caacggctaa aactagatca aaaatacgtt
    48361 cgcgccgatt caaaaagccg tctttcacat cttttatttc agtttctaat cgagcttgct
    48421 tttccttttc cagcttcaac aaaaaaactt tgttgcgaat tgtacggatc tccggaagct
    48481 ttttactgct caaccagata ttcgcttcaa gaacttcctg acggatcgtc cgctgactga
    48541 cattgaactc acttgctaga tgttttgctt ttactggata ttgggctgtc atgattctgt
    48601 gaagtatttc aattgttctt gttttcatag aaatgatctc cttctgattt caagtatatg
    48661 gttccgctta cataataaaa agtagatatt tctgccactt gaagtggaaa gaaagtaagc
    48721 aaagcaaaca aaaaataagc catatgcttt ttcatctcct caacgaaaaa gacatacgcc
    48781 tattattaca aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa gctgtcctgc
    48841 atcaagtaat aagaacaact aaactaaaat agtgcttcct atttttcgtt tggttgttct
    48901 tattatcgca gatgcaagct tttaaacacc atttagtgga gaggttgtga cagagatgtt
    48961 cagccttgag cgataagaag cgattttgaa aaatcgcttc ccatatttta caaaatcgtg
    49021 ccttattgcc gataggctat ctctggcaca ctgtttattc agaaaaagga gctgcgacaa
    49081 ggcttttgtc acaactccgt tcataaatat ttcctgtcaa tccagcataa tcggctgaat
    49141 aaaatcggct tgccatcttt tttctttctc agaaaaatca aaataccata tctgagaaaa
    49201 atactgtcgt tgtgtcttac ttcctgagat caatcgccca tcttttcgat cgactgaata
    49261 atcacagcat ctgaaatcaa ttcggatggc aaaactattt tcgtggatct gtcgataatt
    49321 tcctacttcc tgaatagtta tttttttagt atgatttctt acaccgtctg ctttgttttt
    49381 ttgcagaact ttctgatgtt cggcaaacag cttgtctgta taacagcttc tggctttttc
    49441 aggagtttct tcttcccaag cggattggat cttcatgaat atcttttgga tttccagaaa
    49501 ctgtgctttc ttttctttag ccgttcctga catgcgggac aatatctccg ctttcagcct
    49561 tttcttttgt cttttattct tgacgataga aaaagcagaa acccctccta ctgacaaaac
    49621 agctaatgca agaaatcccg tttgctcata actagaagga cctgtacctg gaccataata
    49681 cccataagaa tctctgccgt aagaacgact gccaaaataa ctgcccccat atgaacgacc
    49741 accaaatgaa cctctgcccg aacttcgacc gctagatgta cctccaccat gaccacccgc
    49801 gacagcgaat ccttgggtcg gcagagcaaa caaacatagg cccactaaca aaatgattgc
    49861 taaataattg atttttttca ttgtcttcct cgttatcttt tattacaaaa aactattttt
    49921 atgatagcct tgctaaaagg aagagtcaat aaccagatgc ataaactgtt tttttagcaa
    49981 ttttgtctta tttagtcatt gagtaacatc ggacaaatgt atgtgtcggc agtccataat
    50041 tcctctatct tcagcatgaa caagcttgca gacaagagat ctgcgcttcc tccaggagag
    50101 atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg taaagcactt
    50161 tgactagcag catgagcaag tttttgcatt tccactaacg ttttctgatc actgcgatgc
    50221 aatacgcatg tgtcatctag cgaactcatc aaaactaaca acatatgcat ttgtttctgt
    50281 acatatgaat cattatctag ctccttagct gcttggattg caagcctgat atgcggatac
    50341 cctaatatcg cttcttcata tgcaccattt acgccatatt tttgcttcac cctatgacca
    50401 tgagtggttt ttttatgacg atattttgta tcaggaaaag cagcaagctt gctgacatca
    50461 tcaagtattt tcgataaaga aaaaggctgt tccatactga ttttttgtgc aatgacactg
    50521 atcaatagcc ccattgccca aatcgctcct ttatgggtat tgatgccatt tgtagcaatc
    50581 agcattgcct tttccgcttt tcgcccaatc tcagcaattc tttctcttaa ctcctgatca
    50641 atagcatgat gccatgctat ttgagccatt tcttcaaaaa aaggagttag cgtcttagca
    50701 gacttaagaa ataaagcata gtccatatcg gaatgtgcac ccgcatccag tgcatccact
    50761 aatccaggtt ttggacttaa tgttacttct gcgatcaatg cttcctctac caacgcagcg
    50821 agttgctgag tcactgcttt ttttcgttcg tcatttatca tgacactcac tccttcttcc
    50881 tactattact tgctgaaacc ttttttgata gctacccaaa tgatatgaag taaccagctt
    50941 aaagcaaatg cacaacttgc cgtcattaga taagctacaa gaatcatgac tcctgaaggt
    51001 aactgcagcc aactatcttt aaacaataat aacagtactt ggaaaacaaa tacattggct
    51061 aaatacgtac gataagcata aacagacaac catttgaaca atggcaattt tggcgaatcg
    51121 tttacaatca ttttggaagc gcctatgaag acagcaaaga tgatgactaa actgtataaa
    51181 ctttggatcg tattcaagta aggtgcattc gcaaagctca agtctcctgg atgtgcgagc
    51241 aatgaacgga cagaaaagaa cgcgataact aaggcaacag gtaagaaaag ataacgtact
    51301 ctgtccaaat attgatagac tttttcgtga taaacaaatg cggctacccc ataaatcatg
    51361 aaactagcaa caaaccgatc taataagtac cagctgtcat gataaggccc agtcaatact
    51421 tgtgtttgat agaacagata ccatgcgaca tataaaatcg ttgaaccaat caatactggc
    51481 catacatatt ttttattagc aaatactttg taaccgagcc aaacaaaaaa cggcatgaac
    51541 aattggatct gcagcatcat aacggtatac cataaatgag gcgctccatc acctgtgaca
    51601 tatttcagta aaaagcttgc agtatccgta tacggagttt gttgttggac tgctggaaat
    51661 agccaaagat aagccgtcgt ccatagaaga tacggaacga ctagttcaaa aaacttttct
    51721 ttcaaaaatt cccaataaga caaatgctga cttgttttta ccatattata aacaattgca
    51781 aaaataaaca ttggtgctgt gtatttaacg atgacgtaaa aggtaccaag aaaagctgta
    51841 tttgttgcgc tgtggctgtt tgccaaagca aagctcaata tcgtttgcat cataacagcc
    51901 gttacagctg atactttctg gaagtcactt acgttgattt tgattttttt gttttcaaac
    51961 atctctttct ctctcttttt ctttattacc aaagttcttt ggttaattta ggtccttcga
    52021 ctgttttcaa tagaattttt ttctctggct ctctcaaata ctcttttagt gaaaaaccgc
    52081 cttgccgagt cactacttgg gtatcgaatt gctcaaaaaa agcaggatgc gattgaatta
    52141 cctgaattgg caattcaatc ggtgaatttg cgtacagcag caaatcaaaa tcactggttt
    52201 cttttactgc cggaatccca gtcgataatt caaatcctaa gctgccgccg attccccatt
    52261 tacatcctga taatatgttt ctagcagcat gaaattgctg ataaacagga taatccatga
    52321 ttttctcttg tctatacgat tcttgttcga tgatgtccca cggatgaatg acttgtttga
    52381 cggcatgttt tgagatttct gttgaccatc tctgattgcg ggcaggtcca cgtaaaccaa
    52441 tcggtagtat ttctgcgtga tccgtcgacg cacgacgaac tgttccgaac atcttttcag
    52501 ttagaacgac ccattcaggc aaggtgaacc cttctatttc atgtttatca aaaaggacaa
    52561 tatcatgtgc ttgaatcatt ttttatgcat cccattcttc gtccattttg gcacggactt
    52621 tatttgtttc gacacgtccg aatgttttgg ctgtttcgtt tgtgtatcga tttgtcagca
    52681 tcgtgtcatt tgtattgcgg acatcttgaa tggcttcatt gattgcttcg atgactttgc
    52741 tgacattttg ttctgaagca gcttggtcgg tgatatcttc taggaaatga tagatcgctc
    52801 ctagttttgt ataattattg atatcgtaag cgatagctgg aactttttca gcagctgctt
    52861 cgatttcagc aaccgaacgt tttgtcacac gtgcagcact ttctttagac atcgcttgga
    52921 cggtaatttc tttattgttt aaagcaatca agcggcttga ttgtaatcca tgtgctaaaa
    52981 atgcacctga aatagcattt cctgcaatca atccaataac tggatgtcca gcttgacgga
    53041 gttttgcata agcagcagca ctattagcca aagccacatg gataccgatc aattcttcgt
    53101 tatagccgta tgcttggctt ggtacatcga tcacaacgat gattggccgt ttcacttttt
    53161 tgtctttatc ttcttcgtaa acgtgatcca aaatttgtcc catgcggaaa ccttcaacta
    53221 aaccaacttc accgtttcgt acacggtaca tcgggttttt aggatcaggt acgattgatg
    53281 tcacgatggc ttcttctatg aatttacttg atttaccata atacacagta ctaatagaag
    53341 aagttggatt tttcatgcca gttagtgccg caaaccaatc gtaaccaaca cttgctgcag
    53401 ctggttcatt tccttctgct acttctggta agtcaattga ttttgcttga tggttagcaa
    53461 ttgcttcatt gtaatcgtca atggccattg gttttgaaag atctaaagaa tcaagtaatg
    53521 ataaataaaa tgctgagctt tcactgcgat gcgtgtcttt tcgttcgtcc attgctgcga
    53581 tgacagcagc tttgattgct tctacactat cagtcactaa ttcatcaatg atttttgatt
    53641 ctacccgttg acgagatcct aatgtattcc aaatcaagtc tttgtcacta gaatcaaatt
    53701 ctcgaacgcc tgcttcttgt tcaataactt ctggtccatt caaaccgact ctcgcttttt
    53761 ttgttgcgat taaataactc attaatgctg aagtaatcga cataccgcca aatgatccta
    53821 cacgtcctgg aatcaaaccg atgactggta catattgttt tagagcaatg attacatttc
    53881 cgatttctga aatagataac agtccgtagt ttgcttcttg caaacgaaca ccacctgtat
    53941 ctagtacaat gactggaaag atctcatttc cttttttatt ttcttctaac acatgttcaa
    54001 gtgcagcaac gatttttgca ccggaaactt caccgatacc gccaccttgg aattttcctt
    54061 ccatagcgat tactactgct tgtttgccat tgatcgtgcc acggctgaca acgatcccgt
    54121 catcactttc agggacgatt ccttgtgctg gtaaacttgg cgcgatgatt tgatcgaatg
    54181 gatcaatcaa ttcacgtcca ttgtcaagta gagcgttcac tcgttctctg gcgtttaatt
    54241 ctacaaaact atttttcatc tgaaagttcc tccattgctt gcgtcaatcg taggtacaca
    54301 actcctggtg tagcaccgaa gtcattgatc acgatatctg ctgaaattgg ataacgatca
    54361 aaaaatctcg ttaatacatt gttccatact tcgccgaagc catcgcttcc tgtacaaatt
    54421 tttacagtag attctgtttt ttctgttgga aacatcaaga tttccaaatc tcctgaaccg
    54481 accactccta catggatttt cttattgatc ggctgtttcg ttggatattt ataagctaat
    54541 tgttccatgg atctctcccc tttttgtaaa acagagcata cacgttctgc ttattcgtct
    54601 ttcttcttcc atctgggtaa atggcgacaa aaggtttttc tcgccaattt tatgaccaag
    54661 ttctgaattt cattggtggt tcatacaggc catctgacca ttcgatcaaa tcatcgatgg
    54721 tttgtgcagc taataatgaa cgtttcgctt cactacgttt gatgcctaga tcttctggta
    54781 atttcacgat gccggctgca cgcaagctat ctagtgattc tttcgtgcta gtcattccaa
    54841 gaggtgtaac accgccaatt gctgcaattg ctgcttgtcg ttcttctttt gtttttgctt
    54901 tgtacaaata agcaattcct tcttctgtca cgatatgtgt agtatcttcg ctataaatca
    54961 tcactggtgc attggctaag cctgcttgtt tttgaacagc tagtgcatct aattcttcga
    55021 caaagactgg ttttttattt gaaccatatg tttcaaccat ttgaacaact aatttttggc
    55081 ctttccctaa tggatcatca ttgtcgcgaa gtgattgcca tgctggtgta gaatgacgac
    55141 gaccgccagg attatgtccc atgttaggcg caccgccaaa accagataaa cgtccttttg
    55201 ttacggtaga agagtttcct tcgtaatcta attgcaaggt tgaaccgata aacatatcga
    55261 tggcatattg tcctgcagct tgtgataatg ttcggttact gcgcatcgtg ccgtctttac
    55321 cgacaaaaaa gacatctggg cgagcagcaa tatatttttc cataccaact tcgccaccaa
    55381 aactatggat gctttcaacc caacctgatt cgatggctgg aatcatcgtt ggatgtgggt
    55441 tcaataccca gttcttcgcg attttccctt ttaaccctag tgattcacca taagttggca
    55501 gtaacaattc gatagctgct gtattgaatc caataccgtg attcaaagat tgtacgccgt
    55561 gttctgcgta gattcctttg attgccatca ttgccattag aatttgcaat tctgtgatgt
    55621 tttgaggatc tcgtgtgaat aaagcttcta attgataagg tttatctgct ttcacgacaa
    55681 cgtctaccca gtcaccaggg atatctacac gtggtaaaga atcgacaact tcgttgactt
    55741 gaacgatcac gataccatct ttgaaagctg cagcttcgat gattgttggt gtttcttccg
    55801 tattgaaacc tgtgtacaag ttgccgtctt tatctgcttt atctgcagct actaaaacaa
    55861 catttggaat caaatcgatg aataagcgtc cataaagttc caaataagta tggatatcac
    55921 ctaatttcat tgtgccgtct tcaatcattt gggcgacacg caagctttgt gcaccagcat
    55981 aagagaaatc gatcttgctt gcgattcctt tttcaaaaat attcaaatgt tctggtcgtg
    56041 agatactaga catgatcaaa tgcagatcat tcactttttc tgcatctact ttttcaagac
    56101 tttctgaaag aaaacttgct tgtttttgat tgtcgccttc taatacaact ttgtcgccgg
    56161 gagcaatcag tttttccatt acttctacga ttttttcagt ttctacatac ttgccattga
    56221 gcaagttgct gattttttcc aatcgctgcg cttttttttc gcgtttagaa gcccagttgc
    56281 ggtttggttt ttccataatt agtcatctcc atttttgata tagaaacgtg gcgtcatcaa
    56341 taccatgttg gtctagattg atcgtacgga tccctttatc tacaccaaat tttgtatgaa
    56401 tcagctttgt caaagtattg ccggggggaa attcaatcgt cagatccatt cctaattctt
    56461 ttgccaagtc catcatttgg ttccactgca cagggaaagc taggttttcc aacaaatctt
    56521 tacgaatctc ttctgcgtcc tgagtggctc ttccatccgt attttttata tacaaacatc
    56581 tagcatcaga tagatggaca gactgcatat acggcttgaa tttcttgacc gtatcattca
    56641 tcaagagaca gtgagatggc acagggactt tcagcagctt cgctgtttgc gcttggtgcc
    56701 gtttggcttc tgccaacgta acggataaac cttcgatact cccagataac gtatgttgca
    56761 tcggacaatt ttcatttgac agataaactg gatggtcagg atcaaatgtt tttgctacaa
    56821 tttcctcagc ttcgcttctc gtcacaccta ctactacgcc cattgcataa ccgcttggat
    56881 aagcttcttt cattaagact gcgcggtgat agactagttc cgctgcattc tcaaatctca
    56941 aagatcgaca agcaacagct gcacaaaaag caccaatcga atgtcctgct gcaatatcag
    57001 gctgaatacc gtttttttcc atttcatcta aataaaatac tgcttttgtc agtaatgcta
    57061 gctggataaa aaccgtatct tgatagtttt cttctttatc aactaaccga taccccgtca
    57121 cattttctaa acgttccatg tacatttcag ggatatccgc taacatccct aacttctgac
    57181 tgccttgtcc aggaaataag aaacctgtac tcatgagcct cgcctcctca gcacttactt
    57241 tttccactgc tatcttagtc aatttctgct ataataaaaa atgccaaaaa aagaaaaaac
    57301 cataacaaaa aggtatggat gggatggata attatgaaat tgcttcatct gcagtatttt
    57361 aaaaaagtat tcgaaacaaa aaatgttacc caagcagcca aagaattatt tatttctcaa
    57421 cctgccttga gccgtgcgat caaacatttg gaaaacgaat taggtgtccc tttgttttac
    57481 catagcggca gaaacattga gacgaccgtt tatgcagaag aattttatcc atatgcagtc
    57541 aagacattag acacattaga agaaggaatc aaattattgt cttcggtcaa tgagaagatc
    57601 gttacttccg tcatattgta tttagaggta gcctctgttt ctattccgaa cttggtccga
    57661 atttttatgg aaaagcatcc tgatatccaa ttaacgatca tgcaacatca tttaccagat
    57721 gatattaccg atcctgtact gtacatcact tcggagatga agcctggcat gaccaatatt
    57781 cctatcatga aagaacctgt ttatgtcgcc attcctaaga aacatcctct tgctaaaaaa
    57841 gaacatctgc aattagaaga tatccaacgc acccccatct tgatgctgtc aaagagcaac
    57901 gccttccgtc atacactgga tgcagcactt gaagcgaaaa gtatcgaatt gacgatcagt
    57961 tcgacgacag atgatcctgc gacattacgt tctattgtca agcaaggatt agctgttagc
    58021 tttttcccaa aagtttcttg gtcttacgat aaaaatgatc ctttcgtctt gcgggaaatc
    58081 aaagatttgc ccttgacacg aacaatttat ttatcttcta gcttcacaga agatcatcct
    58141 ttgatcaaaa tgattgcaaa aacgatgcgg gaattttatt taggaaaata aaaaaaaaag
    58201 agctgggaca aaattcttgt cgcagctctt ttttcgaaca aacggtgttc cagaagtaat
    58261 ttcttagaaa ataagccgaa atttcccaaa aatttgagaa gcaattttcg taaattcctt
    58321 cttatttctt gaagctaaac atttctgtca caacctcttc tctattgtcc tagacattca
    58381 ttgcgttgat ttaaaacttc catcaatcaa atctgctagt tcatctagct tttctatttt
    58441 acgtagatca atagagatcc aatgttcttt attcatgtga taggctggaa aatacccctc
    58501 ttttcctcgc aaaattccgc caagttcagg atcgaccttc aaatcaagaa tattgatgat
    58561 cctatggtca cttgcatcat catagatttt ttcttctgga acagacatca ggaccccaaa
    58621 ccattttttg cttttttgat gtctgaatac gcaataatca ggaaattttt taaaaatata
    58681 ttctggagac aaattgtatg tttcattgat gtgatcgatt agttcttttt tcgttagcat
    58741 ctgcttccat ctctttctta ttgattcgct ttattcaaag ataccacgta agaatagaaa
    58801 aatgaataca agtagttgct aatcaaatac ttcgatattt ttctttttta tcaactctta
    58861 aatgttcaat ccgcctgtgt tggtcttgct accatctact attaaaatgt ttattattgt
    58921 ttgcccacct attttttgat tttagtggta tcatttttca agttcatgat gaaattctta
    58981 tcatcagtaa atgctccaga aaaataaaca atgattgaaa aatcagttga aacttgacca
    59041 ccgtttattt tctccgtatt cttagaatag aagggagtct gattgatgta ttcactaatg
    59101 aaaaaaatca ttaccgaaaa agatattcga cgtcatgttt ctttagtaga acaattgctg
    59161 aatcatacaa agataaccgt aaatgaatta gctgaaatca tagggacaac cgaacgaacg
    59221 attttttctg accttcaatc tattcgctct cagctgcccg aaggctggga catcatcagc
    59281 gatcaagcag gaatcagttt gcaaaaccag caaaatttat taacaaatga cctttgggag
    59341 atttttttca aacaatcagt cagtgtcgaa ctgctgaaaa atctgctttt tacaaaaaaa
    59401 gttgctgtcc ctgattttct agccgaccat gggctttctt atgggacatt gaaaagacat
    59461 gtgactaaaa tcaatcagcg tttagcaagt tacgacttgc agatcgattt gacgaaatat
    59521 acagcttgta ttcttggaaa agagcgtgcg attcgtactt tttatcatcg attgctgatt
    59581 cctttcactc ataacaacta ttttttcgag gattattcga tccatgaatc ccattatttt
    59641 caatttttaa gaaacctcag tcaaactgaa ctagcggttg aaacagaaga gattttcgga
    59701 acatgttggt tctttatcaa tacgatccgt ataaaagcga attgccgatt agattcttct
    59761 atccatacca acagcacact ctcctccctg tatgactctg cattgaaaaa attgtattta
    59821 acggaaggta tttatcttaa agacactgag ctttcttttg cttctttctg ctttctggaa
    59881 agttggaatt acaacaataa ctatggacaa gaaaccgctc gctgcctgca tcattctcca
    59941 tttttagagg tcctcgaaac gtttgtagag gaactagctt cagagctgtc tcttgatcaa
    60001 ctaaaaaaaa catctctgac tgataacttg gctctcctca tattaaaata tcatgaatcg
    60061 ccgatattga ttgaagcatt ggaccgtcaa taccatcgtt ttttagaaac atatgatcag
    60121 caatatgcac acttgtatca acagaaagag aatttactga acgatctaaa aaaagaaata
    60181 gaaatcaatg aaccaaatta ctttctccaa ctcttgtctc tactgatcca acaaacgatt
    60241 ttttctatca aaccgaattt gttcaatgtc tattttattt tccaaggaga accttcatgg
    60301 aaggcgttct tgcagcagga attaaaagat tacttaggaa aacgtgtctg cttcttacct
    60361 atcgaactaa ccgatctttc cactatttct tttgaaaaaa cagatatctt gatttccaat
    60421 ttccctctag attctctaga tatccctatt gtatacattt cttctattcc aacgaaaaat
    60481 gagctcaatc gtttgactga actcactttc aaaacttttt tatagtaacg gctagcgact
    60541 gtattctcta gctgtttttt cttttttgcg aaacagtttg ttcatgatcc ctttttctat
    60601 ttctacaact aagaatagaa gaatcacatt tgctccaatc aataactgtt gtgtcatcgt
    60661 taaacttact gtaccgatca gctgctggcc gactggtaga aaaataacca tcccttgcaa
    60721 taaagcaagg atacctaatg aaagaaaaag cgctttgttt tgcaaaagac cttggttcaa
    60781 tgacggcttg gacaactctc tgcaattgat catgtaaaca gcttgagcta agacgatatt
    60841 ttgcagaaga atggtttgct gtaagctttg cccttcaaac tgcatagcca ataaataggc
    60901 aggaaccatg atcagtaggg agacatagat gatccgaaac aaatgataac cagataggat
    60961 tccttgcttt ggatctcttg gtgggcgctt catgatttct ggatctggcg actcgaaccc
    61021 tagtgcataa gataaagtga ttgtcgttac catattgacc cataaaatct gtactgggga
    61081 caaaggtagc gggcgattca gcataagtgc ccaaatcaag atcaatcctt gagctaatgc
    61141 agttggcaag aagaaattga tggttttttg caaattatcc aaaatccgcc ggccctcttt
    61201 tacggctttt gcaatcgtat ggaaattatc atctcctaaa accatatcag ctgcctgttt
    61261 gcttacttca ctacctttga ttcccatagc aattcctaca tcggatcttt tcaaagcagg
    61321 tgcatcattt acgccgtctc cggtcattcc gacgatttct ccatttttct gcaaagcttt
    61381 aacaattcgc agtttatgtt ctggtgtcgt tcgagcaaag acatcgacta ttggcacttg
    61441 ctgaatcaac tcttcatcag acataagatc caaatcgatc ccttctaaaa ctttggcagt
    61501 atgttttaag ccaatttgtt ccgcaatcgc ttttgctgtg tctttgtgat cccctgtaat
    61561 cattttcact gagatccctg cttgttggca ttcctttaca gctttgactg cactttcttt
    61621 tggcggatcg atcaatccgg cgatccctgc aaaagttaat tgggttaaag tctcatgcgt
    61681 gagttcctgt ttcgaatcta cttttttata agcaaatcct aaaacacgct gaccttttct
    61741 cgcaaattct gcagctgtct gactccatgc ttcttgttct gcagaagaaa gagttgattt
    61801 agcaaataat acttctggtg cacctttgac gtaaatcacc gctccttttt cctccgcatg
    61861 catcgttgcc atatatttgt attcagaact gaacggaatc tttgcttcta tttttttcaa
    61921 aggtagattg gctgtttctg tgtgtcgaat cagcgcaact tctgtcggat tgccacttaa
    61981 attttctgtt ttctgttctt cttttgtttt taattcctga cagtttttca taatggacaa
    62041 cacgcaggct tcttcttttg cagctacatc cacaactgtc atttcatttt ttgtcaatgt
    62101 tcctgtctta tctgaacaga tcactgtcat agaacctaat gtttctaccg aaggaagtcc
    62161 tttgatgatc gccttttctt ttgccatttc atgtactcca acggatagaa tcatggtcaa
    62221 aacagctggc aatccttcgg gtaccattga caccaccaaa gcaatcgtag ccgaaagaag
    62281 gatatgccaa tccataccat atcgaaatgt cgtaaaaata actaagaata ggctcaaaca
    62341 aagaatgcct tgaaaaattt gtttgtttaa tcggttgatt tttttaatca gaggcgtcgt
    62401 ttgttgttcg accgattgca atgcttgatt gattttaccg atttctgtcg tgtttcctgt
    62461 ttctacaacg acaccgactg cagaaccaga ttgtacaagc gttcctgaaa aagccatatt
    62521 tttctggtcg ccgatcgatt cttcttctgt caaaacggca tgactttttt cagatgcttc
    62581 tgattctccc gtcaaaatcg attcttccac taataaatca tgtgcttcga ttaatcggat
    62641 atctgctgga acgacatctc cggcacgtaa gacaatgaca tctcctttta ccagttcttt
    62701 cgcagggatt ttttcttgca tcccgtttga tagtactatc gcttcttgtc ccatcagctg
    62761 cttcaaacca ttcaatgatt cttccgcttt tctttcttgc cagtaactaa cgagtccatt
    62821 gacgatcact acgaataaaa taatacttcc ttcaatatat tcactagaag caaatttcaa
    62881 aaaagaggca atgatcaaca cgatcatcag cagatcagta aaatgctttg ctattttttt
    62941 ccatcgtttt gtctgctgcc ttgcttttat ttcattatat ccttgatcag tcaggcgttt
    63001 ctgtcgttct tcttgcgaca agccttctgt tgatgtttga tagtttttca atacatcttc
    63061 taccgtcaat ctgtaccagt tcatcctatc ccctcctgtt taattgatac tgttattata
    63121 cgcagggaag ttttttcagg cgtatcgatt atttcagggg ggtctgaaat aattcgtttt
    63181 gcaaaatgta tctgggccta actgatattt ctattgctta ttcttttata tacaaaattt
    63241 aagaatacaa accagataac caataaaaaa ggaataaagc gtgtcgtgct ttcgaataac
    63301 aagctgaaag caattgaagt aaaaaacacc aacaaagcaa gatctttata tctttgtgta
    63361 gtgttccgtt tcttttcttt aataaattga acatgtgcac acaagatcga agaccacaca
    63421 aatagaaaac aactcgttgt catgcctgcc aaaagctgaa aaaacaatcg actatcagct
    63481 acaaaaaaat gtagacaaag accggcaaaa aataacattc ctgtaattaa aataccgtgt
    63541 aaaggtacgc ctttcttaga aagcgcacaa aaccaatggg gcaaactttt ttcttttgct
    63601 gtttgatata aaatcctgct agtactgtag actgcactat ttgctgagga caaagaagca
    63661 accaaaatca ttagattgac aattgctgct ccgccttggc aatggatcac ttgcagcata
    63721 gtcactaaag gactttcttt taaatctaaa caatgccatg gaacaacaaa taaaatcgac
    63781 aaaatcgttc ctatatagaa aaagagaata cgccaaggca gacagttgat tgcttttttc
    63841 aaattctttt gcggatcact tgtctcgcca acggttaagc cgatcatttc aattccagca
    63901 aaactgaata aaaccatagg aaatgagact accattccag caaagccaaa cggtagtaat
    63961 tcatcgatct gtccaaaatt ttctttgatt ccgatagact gggtatgaaa atctgagaca
    64021 ctcaatccta tacccaataa gataaaaagg aaaatcccca agatcttgat gattgagaaa
    64081 aaagattcca attcaccaaa ccacttgact gcaacaaggt tgactgccat taagatcatt
    64141 agtgcgctac ctccgcttat caaagctggt acatgtacaa accagaaatg gatatacaat
    64201 cctattgctg ttaattccgc aagtccagct ccaagccagc aaagccaata agtccaattg
    64261 acaaaaaatg cccacttttt gcctaaataa tagtcaacga aatcactgat agagttaaat
    64321 gtactatttg ctaaaaataa atcacccatg gcccgcatca aaccataaat agccaaaccg
    64381 catatcaaat aaacgagaat aacagctggc cctgctgatg caattgcctt tgctgaacct
    64441 aaaaataatc ctgttccaat tgcgccacct aatgcgatta actggatatt tcgattagtt
    64501 agagtccgtt tcattgccgg tttttccatc aagttcacct ttttcttttt caagcagcat
    64561 ggcgacttcc tgttcccaag aaggtttctt tattttttca atacagagtg gtactacaaa
    64621 caaaatgaca ctgatggctg tgactgcaat tagattgatc catgttccag ctttcatcgt
    64681 cagtccgaca gaaaatccga ttgccgcaaa taaaacaata acgacagtcc aaactccaag
    64741 attgctcttt ttctcttttt ttccaattct aaatggtcga ttcaactttg catgtgtata
    64801 acgcaatctc aaaactgcga tccccatgat gacatagacg atacagtaga taacagtagt
    64861 tgaatttacg agcatcaaaa atgcttgatt gacgccagga atcagcaaat aaaaaagtgc
    64921 aaagattgag ataatgacag actgagtcaa aataacggat ttggatacgt caaattgatt
    64981 cgtcttccaa aaattgaatt ttggtggata cgcacctttt ctagcacttt ctgtcactgt
    65041 ctttgctggt cctgaggccc aagcagataa ttggactgct acaccaatga agacaagtaa
    65101 actaaacaga ttcacaataa gatgtggcaa accaagaatt tggcaataaa tcgaaataga
    65161 ttgcgcgata ttattcaatt ccatttgtcc ttttggtacg acatttgcta caactaaagc
    65221 attcaaaata ttgacgaaaa atataaaaat taacgcggca aatactccgc gaatatacgt
    65281 tttaactgga ttttccaatc gagtgatata cacagaggac atttcgatac cagtaaaaat
    65341 aaacatgatc ggcgcaaaat aaacaaatga ggatgcagtc gtagtatctg gtattaattt
    65401 gtcccatgaa aaactgccta atgttccggt tggtacgatc ccaactttga tccacgcagc
    65461 aaaccctaat aagagcatca tcgtcaaagg cacgtacaat cctaaccaaa caccgacttt
    65521 tccgccgatt tttgccatat caaatttcaa attcaaaaga gtgatgatcc aataaacgac
    65581 taaaatgaca actaatgtaa atttgctgtt caaaccaagc ggcacattat cgataatatt
    65641 accaaacaac ggagcgagtg ctgaagcgac catcaccatc ccaggaaaca tttggaccca
    65701 taacagccat gagacaacga acccccattt ttttcctaga gcattcgata cccaaagttc
    65761 tggaccacct ttttggggaa acattccagc aaattcacca gagatcaatg agattggaaa
    65821 agcgtacaat aatactgcaa ccagcatgta aaaaatcatc gtccagctcg ttgctgctac
    65881 atctggaata tttcgtacac ttgctacaag ggcgcaggtc attccgataa aggtcatggt
    65941 cgtgatttgc tttgatgaac cagtatcttt catctttatt ccttctttca atagcgacat
    66001 atgtttttgt tttcttttat ttttgttcaa gaacagaaat gacaaggttt catcgattca
    66061 gtacaaaacc cagtatcacc gaaacatcca ttgaacgatc ttagtgaact gggtttgata
    66121 catgaaacga acgatccaac ttatagaacg ttttataaaa aagaatatct tggcgaggaa
    66181 tatatgaaaa acagggggaa atacaattgt tttgatgaag caaataattg tctgttctcc
    66241 atatgtctct tgttattctt tcacacaaaa atcagttcta ttggatgatt tcgtttagtt
    66301 tcttttccat tgctttggcg attttttttg tataaaaatt gaaaattcga tcatcattca
    66361 aataaggagt catgatagcc gcacgtagca gagtgatttt ctgttctttt ttccattctt
    66421 ctttcgttag acccattctt tcaacaaaac gaatcggaga atcaccgtaa tcttcttgtg
    66481 taaatgtagt atgagaagta ataaaccgtt caccataaac atcgccatcc atataagaag
    66541 accgatcgaa cattttttca tttaattcat tgatagcgtt caaatctgta cagccttgtt
    66601 ctttgaatac ccaattgacc atattaaaat caggatgatt caatggatag acttcgattg
    66661 tttttccttt aacagtaaaa gtcagatgat cgagaaattc tctgaatctt tgagctgcct
    66721 cgattgaagc cccaatcaat tgaccatatc cggtcacatt caatggcaat acacgatgtg
    66781 ctgtccagac tgcggcggcg gttgctccag cttttgatcc ttccaaaata taagctccta
    66841 acatatctgg agcctttact gatttttcaa acacatatgg cgcgaaataa gaaatgatat
    66901 ttctcatatt tttatgtttg attacgattc ctccagcagc ataagggaca tagcccatct
    66961 tatgaggatc tatcgtgaca gaatccgctt ctgaaatagc tctgaaccct tcatatacct
    67021 ctttgtcaat cgttacgcag tggtggaaaa catgatgttc ttcaaagaat tcagctaagg
    67081 aagcatacgg cacaaattct cctgcttcgt ttagaaacag tgaacgcgca tagccgccat
    67141 atgcggcatc aacatgtaaa tagaaataga tcccttcatc ttttaacctt tctcttaatt
    67201 ggacgatttt atctacactg tctacttgac cttcttctgt cgttcctacg acagcaacca
    67261 cacctagaat tgggattttt tgtccagcta attcacgaat tgttttttcc aaagcattaa
    67321 tatccattcg ataatcctct tgaactggga tagcaaccat ctgatctaag ccgactccgc
    67381 aaatatctaa cgccttcatc caagaatagt gttttgtttg agggaccagc catttcccta
    67441 atctttggat attttttccg ctacgggaag aagctgcttt gacctcatcc atttcttcgt
    67501 ccgtaaagct ttcggtcatt tccaaaattt cttcaacaga taaattcaaa agttcccact
    67561 cagacatttt tttcactttt tcaggcagca cttccttgac agctaaagga atcgatttga
    67621 tgcaacgggc gtaccataat ccttcaagat tagcaataga accatctgct gcaatgtgtc
    67681 cccatccgtc tgtcatattg aataatgaag caaaatcctg tcctacttct gcttccattt
    67741 gcgaagttgc catagaagat tccaatgcaa cattatttgg gttccacagc atcgcatagt
    67801 tgtaagctaa taaagcaggc atcaatgttt cagcattcat ttgtccccag taacggcctg
    67861 ctgaatgcca tgggatcgat cctgcgcgta atcgctgact gacttcatcc aagacctcta
    67921 tcatatggtc gcgggtggct agataatctg gagtgagctt atctccttcg ctaattgcag
    67981 gcatatctga aggaatataa ttttctctcc atccaagatg ttcatctacc atcttgttca
    68041 acaacatttt ataaactggt ccattttcag ctttgtcgcc tataaagacg gccttgatat
    68101 ccatatcttt catttttctt ccctccatat tttgttggtc aatgtcttgc agatacatgt
    68161 gtttatctgt aacacctctt cactatagcg ccaaaaatga gaaatttttt tttataacat
    68221 caaatccacg tagattttta caaacaaaaa taaataaagt cggatttttc tgataattca
    68281 acgcaaaaaa gcgaacaatt tttcaataaa tcagtcttct aaaatagata acctaaaaaa
    68341 agcgaacaaa caaataaaat gactgtcctt cattcactaa acatattttt atttttttat
    68401 attgttaaaa attcacaatt tattttttaa tagaattatc tgttttccaa attttataac
    68461 ataaaaaacc ttttttaacg gcatttatat gctttttcag aaattaactg atgatttcaa
    68521 aaagaaaaca tgtcataata tttcccatat tgtgcaaaaa aatacaaggc ggaggctcta
    68581 ctatgattga agaatatatt gagaaaaata ttttaagaca gctgttttta tgtgggcaat
    68641 tctatgtaaa taaagaagtc aatctagaaa agctctccaa tctcctgcat gtttgcaaaa
    68701 ccactttact caatgatatc aataacataa aaaaagaatt tgaagaacag atcgcttata
    68761 cacatcggga aaaggaccgt tacactttat atttttcaga acatatacct cgatgcaaga
    68821 tcatgcagca attgtctcaa aattcgttgt ttttaaaaac ttgtctgctt tatttggaag
    68881 aagatgaacc tgattatctt caattaactg aatgtgaatt catctctgtc agcaaagctt
    68941 attcattaaa aaaacaagta ttagcttact ttaatgactg cgggatcgaa atcgaccgct
    69001 actcccctcg attcactgaa atggaaagac gtctgctgct tcttaatgtg tcgtatcgtt
    69061 taggcggttt taattcttgg gagctacctg aatcgttttt tgaacgtgcc gatcgtttca
    69121 ttgaatccgt aacggaaaat agtggcagat tttatgataa agaaaataaa gaaatcttat
    69181 cgattggttt tgctatcagt ttcctacgcc agcaagtgtg tgctgtgaca atagattcaa
    69241 aatttatcga agaaatcaaa aaacgtccta tttacaatta tgtcgaatca gcatgggaaa
    69301 acactgattt ccaaacctat tataaaaaag aagaattcgc ctttattttg acactattca
    69361 atctttgtaa ttatggtttc cattcttatc agttgattgc ggaagacttt caacagctac
    69421 accaagtgtt tattgacaat actcccgaaa taaaagaact agttgctacc tttgaatctc
    69481 acttcaatca agaattattc ggaaaccaac cgttcgaacg tgcgctgatt catctgatgc
    69541 gttcggcttg ggataactat caattattca tgccagaaaa attttatctt ttaaatgagg
    69601 aacagacaaa tttatataaa gaagtccaga caattttttc cagctggtcc agccagttac
    69661 cttatgatct acggttgaat cctaattgca tgcgggcttt tgtgatcgaa ttgagcggaa
    69721 ttctccgtct gacaaaagaa catctgacta tctatatcgt cacaaattcg gatgttcatt
    69781 atctgattta tcgggaagcc ttggaagctg taactacttt cgattttcaa gttgcaccaa
    69841 caatttattc aagtatatct gatataaaga agtatgcgca gcagtcctca aaccgggtat
    69901 tatgtgaacg tacattatat acaccagatg cggtccaata tgaaaatatt attcctattt
    69961 cgatcaatac gatcgatcga gctatcattt cagctgtgca aaacaaataa ggggctgtga
    70021 caaaaatcat gtcacagcct cttatttgtt gaagatgccc atttctgtaa caatctcttg
    70081 atacacgtca ggctcacaac ctcacttatt ttggacatct aggggtccga tctctttatt
    70141 taaaaaataa gaaatgatgg ttcggataac taccaacgcc gctagttgga aaatatcctg
    70201 caaggtaggt ttgataatcg attcgatgat cccagctgaa acaagaattt ccaaacttag
    70261 aagtatatag cctcccaaca tctttttaat agcattgttc tcgcttactc ggtcgctttg
    70321 cacttttgta tggacaaata aatatttcaa aaaatccttg attgatagac aaacacccca
    70381 aaccaataaa cagatagcaa agatatcaag tcccagtaca agtttttcga aatatggaag
    70441 aagaaactgc atcaactctg tcgtcatcct acctctcctc ttctttgttt ttctttgtct
    70501 tctatttcct gattcaagaa ataagaaatg actgttcgaa tgataataat aagtgcaagc
    70561 ttcaaaatat ctgtccaagt cggcttgata actgactcga tgatatcagc aacgactagt
    70621 acttccaagc ttaacaaaat ataacttgct agcagttttc ttgtatctgc attacgagta
    70681 ttacgttctg catagttttt cttgacatga gaccaatcaa aaagtttgaa tagaacaaga
    70741 acgatcccga caattaagat accgatagaa caaatattta aaatagcaat gatccattct
    70801 agaaattcaa ttaaatttga gcgaaacata tacgctacct actttctttt aatttgttcc
    70861 tattctttta ttgtaaaaga aagttacctt gttcgtctat ttaaaagttg ttctaatgat
    70921 aacggttcgc ttaaattttt ctttttcact ctcccatttt tctccctcca tccataaatc
    70981 gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc ttagtccgtc atcagctttt
    71041 caaagaagca ctgtctcggt gctttttttg tacccaaatt tctaaaaata gaaccataca
    71101 cctcctcaaa tcttttcttc ttacctaccc tatttttatt tacaggaaaa tcagacactt
    71161 cttctttttt gtcaaaattt cctttttctt ctcgtcctat catttggcat tctaataaaa
    71221 acgaactatc cttggatata gaaagagaaa acaaggaggc gtttttcatg agtaaaaaat
    71281 taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg cgctgcatat ctaggaaaaa
    71341 agacaggact attcgaagat gacagtcatt tatatgatga attcgaatca atctagcatc
    71401 ctatttagtt gaatcactac ggacatactt ggaagacatt ggaggaatca caatgagaaa
    71461 aagtaaagca ataatgattg gtgccggtat tgccaatatg gcagcggctg tttatttgat
    71521 tcaagaaggc aaatggaaag gggatcagat caccttttat tcattggatg atcacggttc
    71581 aaatgatggt gcaccgaccg acactgtagt ggatgaatat tggaataaaa accatccatt
    71641 agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg cttaattacc gtacctatgt
    71701 agatttgatg gatctgttaa gtcgaatacc ttctgttact gagccaggga tgactgctga
    71761 agaagatacg agagattttg atgcaaaaca ccgaaccttt gacaaagcac gtttgatgga
    71821 aggtggcaaa ggaatcattg atggcggaaa actgggattc aataataaag accgtattct
    71881 tctgactaaa ctgattgcta tgcctgattc agaagaagaa aaactagaca atatcactat
    71941 cgaagaatat ttcaaagatg atccacactt ttttgaaaca aacttctggt ttatgtggga
    72001 aacaacattt gctttcagaa cacgtagttc tgctcaagaa ttgcgtcgct atatgcatca
    72061 aatgatttat gaattcactc aaatcgaaca tctagtgggc gtcaatcgaa cacgttacaa
    72121 tcaatacgaa agtattatgc ttcctttgat cgagtacttg aaagaacaag gatgcaaaat
    72181 cattttgaac cgccgtgtca ctgactggga attcaaagaa acaccgatgc aagatgaaat
    72241 cactgtgact ggtttgaaaa ttaccaatac actgactcaa gaagaagaat acgttgtggt
    72301 cgatgaagat acagcagtta ttttcactaa tgggtccatt acagattcag ctactttagg
    72361 agattacgaa acaccagctg tagaaaatat ggattatggt gcagcttcta gtctctggaa
    72421 aaaagccagt gaacgtttct acaacttggg aaatcctgac aaattctttg ctgatcgaga
    72481 tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac catttgttgt tgaatgaaat
    72541 cacccgtatc acgactcaag aaccaggaaa tgccttgaat tcatttattt caaccacacc
    72601 gatcacgccg ttaggccaaa aagatgtcaa tatgtctatt gtcgtgcatc atcaaccgca
    72661 ttttacgtct cagaaaccaa atgaaacagt tatttggggt tatttccttt atccaagacg
    72721 acgtggcgaa tttgtggata aacagtatat taaaatgaat ggaaaagaaa tgctagaaga
    72781 actgattgga caactttcaa aagtagatcc tggaccagtg aatattcgtg aaaaagaaac
    72841 agaaattttc gacagcgtaa taaataatat ccctgtctat atgccttatg cttccgcgtt
    72901 gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt cctaaacatt cgaccaatct
    72961 ggcctttaca ggagaatttg tcgagcagcc ttatcagatg atctttactg aacaaagtgc
    73021 tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt attcctatgt cccgtcttgt
    73081 caaaacaccg cgttacgaca aagatattcc tactctagct cgtgccgcaa aaaaaatgtt
    73141 tgaataatca agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa caaccaggct
    73201 aaaacagctt cttatgtttt tgatttggtt ggacttatta ccgcagatgc aagcttttga
    73261 acaccgttta ccaagaggtt atgaaaaagc tgccctgcat caagtaataa gagcagccaa
    73321 gctaaaacag cttcttatgt ttttgacttg gttagactta ttaccgcaga tgcaagcttt
    73381 tgaacaccgt ttaccaagag gttatgaaaa aggcgtttag tttcgagtat gaagaaaaat
    73441 tttgtgaaaa tggttcctca cgtttttgca taaatttgac ttaataccga gaaactgatc
    73501 ccttctgtca taacctcttt ttatttatgt ctgtttcttt ttgaaaacaa ctattttttc
    73561 aacgtttact ctcttgccaa tccaacatca tctaacggat acaactattt caatgattac
    73621 cattacacta aaaaatcgga aacacacctc tccgaacaca aatacataag actatagaat
    73681 gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt aagatctcct ttaaaatgaa
    73741 attatctgta gtactaataa tgcagacaat tttatgaatc tgaaaggagc atctccaatg
    73801 aaagcagtca cttggcaagg aaaacagaaa atggaaatca gaaacgtaga tgatccaaaa
    73861 atattagaac caacagatgc aatcatccgt atcacagcaa cagccatttg cggctccgat
    73921 ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag ggtatgttgt aggacacgaa
    73981 ccaatgggaa tcgttgaaga agtgggatct caagttcaaa cattgaaaaa agggcaacgt
    74041 gtggttatcc cttttaatat tagttgtggt cattgtcatt tctgcctgaa tcacatggaa
    74101 agccaatgtg atgaatcaaa tccagatcag ttatatggcg ggcttttcgg atttggcaag
    74161 ttgaacggta atcattgggg tggtcaagcg gaatatctaa gagttcctta tgcagactct
    74221 acttcatttg ttgtaccaga taatgatctt cctgatgaaa aagttttatt tctatcagat
    74281 attttgccca cagcttattg gagcgttgaa aacgcaggag taaaaaaagg cgatacagtt
    74341 gtgattctag gttctggtcc agtcggtcta ttcgctcaaa aattcgctgt tatggctgga
    74401 gcagagcgcg taatcgcagt agatccagta cagcaccgtc tagataaagc ggctaattac
    74461 aatggggttg aaacttattt cttagaagac acggcacaag ctggaacaga attgtatgaa
    74521 ctggctaaag gtggcgcaga tgtcattatc gattgtgtag gaatggacgg attagagcct
    74581 gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaagcg gcacaatctc tccattacag
    74641 atggcatcgc aagctgtcaa aaaatttgga acagtccaag taactggtgt ttatatgact
    74701 ccagcttctt cttacccgct gcaagaattt tttatgcggg atatcgctgt caagcatgga
    74761 caggcaccaa ttatccatct aatgccaaaa atttacgcaa tgattgcaga aggactattc
    74821 gatcctacac aaatcattac tcattcgctg ccactatcag aagcagcaca agcatatgag
    74881 atctttgaca aaaaagaaga taaaaatata aaagttgttt tgaaaccttg aattcagtaa
    74941 aaaatacagg tgattttctt tatgcccaaa atcaaacgaa atacccatga gatcgaaata
    75001 ggaaaagaag aagatatcga aatacgagga cgtcgcttcc gcttctattt ccaaaatcgt
    75061 tacacattga tctcactggc tgtcgattta ttaaccggga ttttctatat ttttggaagc
    75121 cttgcttcct taactaaaat ccccgatcga tatggcacct atttttattt ggctggtgct
    75181 attttcttga cgattcgtcc tattctcaga atcgtaagga acatttttat ttacgacgaa
    75241 aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat gagaattttt caaatctcat
    75301 gcctcttttt tcaccctatc gtatcacttt gatacagacg agcggtcgtc ccactttctt
    75361 ccttgaccat tgtcaaaaat gtccactcgt atcgttcata aaacgtggta tgatctgtaa
    75421 ttaagtaaag tttttcgtac ccaagttgat gcatttcttt tctgatttcc gctaatatcc
    75481 gcttggctat cccctgctta cgcttttccg gttcaacaaa cagggcacaa agatttggtg
    75541 tcagatcagg gcggttatgg aagtcgtttt caatgacgcc cgctcctccg acgatggttt
    75601 ctttttcatc taagaccaca taccattgag gaatactttc cttattagcg attccagtac
    75661 gaatactttc ctcatacaca acagcaggaa ctccccattt tgatgaaaac caattcgcgc
    75721 tttgttttac taattctggg tgttctctta atttgatcca gttatatgta accaatgttt
    75781 aaacctccga ctaaaataaa acgtttacaa aaattataca agtctttatg aaaattagca
    75841 atgacaacaa tagtgatgta aaaaaaaaaa cggaaaacaa tcacttgttc tccgtttttt
    75901 gattaatcaa gcatctaaag cttcttttct tctttcttgg atcgtagcta ccttgacgaa
    75961 cggtagatag atcaaaatag aaagaaccaa attgacagcg gctagtacac cacctgcaat
    76021 actttttgtt gccaatacac cgccaataat tggtggagtt acccatggag gcataacggt
    76081 agctgctggc actaaacctg tagaagtcgc aaagtaagca acactaacta agaccattgg
    76141 tgtcaaaata aatggaatga acatgatcgg gttcaacacg ataggtaacc caaacatcgt
    76201 tggttcatta atattgaaga cacctggcgc aatcgacaag tttgttacaa ccatatatgg
    76261 tttgttttta cgtcctacta aataaatagc taacagtaaa cctaaagttg cacctgttcc
    76321 tcctaagttg acaaagctgt caaagaaagg tttgttcacg ataaacggtg cgctatgtcc
    76381 agctgcgatt gcattaacgt ttgcttcaat ggctggcgca ttgattgttt gcatcaaagg
    76441 atcgaccata ttcgctccgt gtaaaccaaa gaaccataag aatggtgtga taaatgcaag
    76501 cagtaaggca gatggataag agtttgcaag tcccataaat ggttcttgga ccactgtata
    76561 gaaagaacct acgatatcat caacaccgaa tgcagcaaaa attgcagcaa ctaaagcaaa
    76621 cgcagagata gtgatcattg ctggtagtaa tgaagcaaat gcttttgcaa ctgccggtgg
    76681 cacaccatca ggcattttga tgatcaattt ttcatttccg cttaaacgta caaagatttc
    76741 tgttgaaata atcccaacaa taatagcaat aaacaaaccg tttgagctca tacctaatgc
    76801 tccgcctaaa gcgaagaaag agcctaaaga aatcacacct gcggaaacac cgtcttttcg
    76861 gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc aagacagaaa gaatggcaaa
    76921 tgtaccgttc caagcagcag ccccgaatcc tttccatgat tcaccaccaa aaattgagtt
    76981 catcaattct tggaacccgg gaatcggtaa gttattgatc attactgcta atgctcctaa
    77041 gatcattaac ggcatactca ccataaatga gtcccgaatc gcaactaaat gccgttgtgc
    77101 accaattttt gatgctacag gtataaaatg tttctccata aatgcaacaa atttatccat
    77161 aaatcttccc tcttttcttt actgtagatt acgcgttcaa tcgacgatac agattaatga
    77221 tctcttttgc taaatctgta aaagctatag atgtcatcag atgatcttga ctgtggaccg
    77281 tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg tgtcagcatc tctgtttgag
    77341 agtgatgtgc ttggactaaa gattcctctg catctttgat tttttcatct gctaaagtga
    77401 aatcaccttt tttcgcagca gcaatcgctt ccattgcatc gctttttgca tttccgccaa
    77461 acatgatcag acccatgatg gcttctagat ttttttgatc ttccatttat tgtatcctcc
    77521 gataattttt attatttgtt tattagtttt tctgcttggt cgagtacttt ttcaccattc
    77581 atcatgccat agtcagccat attgattaca tctaaaggaa tgcctttagg tgctagcttt
    77641 tcagcaaatt gattttgcat aaagcgtact tgcggtccta aaagtaaaac gtctacattt
    77701 ttagattcta gttgattatc cgcatctgaa gcagatacag caaagatgtc tgcttccatt
    77761 ccttttgctt ctgctgcctt ttgcattttt gtgactagga gacttgtact cattccagct
    77821 gaacatacaa gcatgattgt tttcttttcc attttaaaca cgttcctttc tatacttaca
    77881 cttgtatata agcaagtagc gtgccaactt taaaagcgct ttaaaaagtt gatatcatca
    77941 catttaaggt ctataccaag ttgcatacac actaaaaaag aaagcgcaca ctaaacacac
    78001 aaattaattt ttattacctt ttttctttta acgtgcacaa aaaaagttgt gacacaaaac
    78061 ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt gcatctgcgg taataagtcc
    78121 aaccaagtta aaaacataag atgttgtttt tactttgcta ttcttattac ttgatgcagg
    78181 acagcttttt cacaacctct caataaacgg tgttcaatca gctgaccctg ggtattagct
    78241 caaaacgctg gattcacaac ctcttttatt tcaaataacc agtaaataca atgaacatct
    78301 acaaaaaact tctatttatt tatagatttc gtcaggcaaa tccaattctc tcttatcaat
    78361 caaagtctga taccacgaag cagatttttt caatgttctg cttcgttcat cgttcaattc
    78421 gattcgaact aatccatatc gattcttaaa tgcattcatt ggtgacacac agtcagtgaa
    78481 tgcccataac atatagcctt cgcaatttgc gccttcttct gttgctttga tcaagtaatg
    78541 caaatgctgt ccgataaatt cgatacggta atcatcttgc acgattcctt tctcatccat
    78601 atactgttct tcatattctc ttcccatacc gttttctgtt atcagccaag ggatattatg
    78661 ataattttct tttagataca tggctatatc atacatgatt ttcggataga tttcccatcc
    78721 cctggaatta ttcattttct tcccaggtaa atcaaagact tcataatatc gttcaggatg
    78781 gaaaggtgtc tcttcattcc attgataacg aggggcgcga acgcgtttag gataatattg
    78841 attgattcca aggaaatcta ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg
    78901 atcgaaataa atcgattgtt tttgacacaa ctccaggaac tcttctgtgt agtatccttt
    78961 taccatcgga tcaaaaaata ctcgattata aaacaaatca tacatataag cagctttttg
    79021 gtcttctgga gaattggatc gagcatagac catttcagga tttaaaatac agccaatcct
    79081 tccaccataa tttccgctgc ggtaaatctc tactgcttta gccgttgcta aaactttatg
    79141 ataattccac atcatccact tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa
    79201 atagcaacgc gtttggggaa cgattggttc gttgaatgta aaccagtttt ttaccctatc
    79261 accgtaacga tcaaaggcaa tttgagcata cttagcataa aaatcaacta cttttcttga
    79321 agaccagcca tcatattttt ccaaaagaaa agctgggact tcataatgtt ccaaacaaat
    79381 catcggttct acgtctgctt cgatcaaaga gtcgatcatt tcacttatat gctgagcata
    79441 ttcttcatct actatacagt tctcataatc agtaaaaaaa cgagcccaat tgatagaagt
    79501 ccgataatgt gtcagatgga tggatttcat cagatccaca tcctgctggt attgttccat
    79561 aaaattagtt gctacggctg ggccataccc attatgccaa acaaaatgtt catttttata
    79621 ccagctatct ataaacgaat cctgtccttc tttttttcct ttccaacctt ccgtttgcca
    79681 agcagaagat gcagcaccaa gaataaaatt ttttggaata gtaattttca tgatttcctc
    79741 ctgtttttta gatcccatca ttttccacac gatttgctac ttttacaaat ggcagataga
    79801 tcaaaataga aacaataata caaacaagtt gcgtaataac tgcaccgata tttcctgctg
    79861 ttgccagata agcattgacg atcggcggca ttggccaagg gaccattaca actgctttcg
    79921 ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc agtgataaga ggcgttaaga
    79981 taaacggaat agctaaaatt ggattcagca tgatcggcat accaaaaata actggttcat
    80041 tgatgttgaa gatcccagga gcaagggata acttggtgat ttgtttcata tcttcccgtt
    80101 ttccaactaa caatactgca atcagtaaac cgatcgtgac tccagacccg cccatactca
    80161 tatacatatc ccaaaatggc atagtaataa tgttcggtac ttcttttcca gcttcaaaag
    80221 cttccgtatt tacagcaatt gccgctaaaa gcaacggttc ccgtatcggc ttcaccattt
    80281 gatttccatg gataccaatc acccaaaaga cttgcgcaat aaacatcaat agcaaaatac
    80341 ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac gacattgtaa atgatatcat
    80401 aaaggtacat tcctgtaagt tctttgatga taaatcccgc agccgcgata cccgatacag
    80461 tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac attcgttggc acggtatcag
    80521 gcattctcag ctgtagtttc ttctgtttcc tcaaccagca ataaagttca atagaaagga
    80581 tggagaccaa cattcccaaa aataaccctt tcgtatctgt atattgttta gccaatacgt
    80641 tttctactat aacagtctga ttttcattca ctaattcata aacagttgga ttaacagata
    80701 aataagacat cactgcgatg ataccaggga atacaccatc cactttattc agcttggcta
    80761 attcgatccc tatcagaaaa acagcataga tggtcaagaa acttagtgtg acatagctga
    80821 tggcagatgc gataggcttt aattcttcta aaaaacgcaa gaaacttatc tgtgctaagc
    80881 cattttccga gtcaaagacc attgctgaaa acaatgtgcc aaaagcaccc gtaataatta
    80941 ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat ataccttaaa ttattgattc
    81001 tcgttgccac gttccctaaa acattggcta atttatccat aaaactcatt ccagatcccc
    81061 tcattcctgc ttcttctcct tgacgtcatc tctttttgtc tattagtcag cgcttacatt
    81121 tcgatgaaca gaaactattt actcctcttt tttaccaaat ataaaacaaa aattcatctg
    81181 atgtatttat cataaaatac atcagatgaa ctgtcaatta gtttcttttt ctcctattat
    81241 tttctgtaat tctctccgat ttattgccat atggacagtc attgcatcaa acgcagcaag
    81301 tgcattccgc ctttcgatgg cctgaacgat ctctgtatga atatcgatcg tctcgatttt
    81361 cagatgctgg atatcataat ttttattaaa caaagcaatt gattcattaa tgattgggat
    81421 aatatgcagc aaggctacgt ttccgctagc ttctgcaatc atcgtatgaa aatcgatgtc
    81481 taattgaaca tgttcttgtg tgccttctcg aaaagaagtt tcaatctttt gcgttaattt
    81541 tttcatttgg acgatttgat tcttcgttgc atattccgct gccaaacctg ccatcttagg
    81601 ctccaataaa tatctcagtt caaataagtc tcgtatcatc cgatcctgat cttcaatcaa
    81661 agaaaaaccg agtgggtcat tggcaatccc tcgcttatca ctgacaaatg tccccgctcc
    81721 ttgccgaact tcaaggatat tcctggaagc tagagcccgc acagcttctc gcaccgtgct
    81781 tcgccccacc tctaaataat cggacaattc atattcattc ggcagtttat ctcctacttg
    81841 caactgcatt tcctttaaat atctaaggat cttgtcagta gttacttcta ctagggtttt
    81901 attttcttcc aaaccacata cctccttttc atctgatgta ttttttcgta attattataa
    81961 cacgtttgaa tcatctgatg attttttgta tacaaaattg attaatccgt tcatagacat
    82021 acaaacgaag aagatattta ccataaaaaa gactaggata aaattcactc ctagccttcg
    82081 atcattttta ttttgaacga caaaaaatat gatgttcaat aacagacgat cttcattcga
    82141 ttcttgatgt catcttttgt tttttttaat atatggataa aaagtttgat tacatcgttt
    82201 ctattctaga atttggtcga ttctcaattc ttcttctggt gagaaatcta aattatccat
    82261 cattcgaaca ctttcttgta actgacttaa ccgacttgct ccgaccagta cacttgtaac
    82321 agatttttgt cgcaaattcc atgcaagggc catttgcgct aatgattgtc ctcgtgatac
    82381 ggcgattgtt tgcagtgctt tgattttttc cagagttgat ccaacttgtt cttctgaaag
    82441 gaatggtatt tccttacgat gtgctctaga atcttcagga ataccatgta gataacgatt
    82501 tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc cctagccctt cttcttctaa
    82561 tactggagat agatcgtctt ctagtagtgc acgagaaaac atattgtagc gcatctgatg
    82621 aataatgaac ggtgcttcct ttctttttaa aatttcagtc attttttttg tatcttctcc
    82681 attatagttg gatataccaa tatataatgc ttttccttgt cttacaagtt gcatcaaagc
    82741 ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga tgatgataga agatgtcaac
    82801 ataatctagc cccattcttt ggagactttg gtcacaactt gaaatgatgg atttacgaga
    82861 gccccattcc ccatatggcc cttcccacat atgataacct gctttgctag aaataatcag
    82921 ttcatcacgg taggcttgaa aatcttctct aaaaatacgc ccaaagtttt tctctgcagc
    82981 tccagcaggg cctccataat tattcgcaag gtcgaaatgg gtaattccca tgtcgaatgc
    83041 gcctcttaaa atctctcttt gattatggat aggatcataa tcaccaaaat tttgccataa
    83101 tccgagagat aaaatcggta atttcaaacc tgaatgaccg gatttacgat attccatttt
    83161 ttcataacgt gcactatctg caatatacaa tttttctcct cctcatcaaa tctttttaaa
    83221 acgctttctt ttttctattt ttagtttact acattttaaa gaaaaggagt taatcaaaat
    83281 gattttttcg tatatttttt tgccatttgt tttttactcg ttgatagatc agaaattgat
    83341 tacgtgcttg ctggattttt ttataacgtt tcggttgttt tttgtaaaat tgctgatgat
    83401 agttttctgc tggccaaaac gtaccggctg gttcgatttt tgtgacgatt ggttggtgga
    83461 aagttcctga actgtctagt ttctgttttg attgttccgc taattctttt tgtcgttcga
    83521 cttgataaaa aatcactgga cgatattgct tccctctatc ttgaaattgt ccaaatgtat
    83581 ctgtgggatc agtaatttgc caatagattg tcagcaactc gctataactt atttctcttg
    83641 tgtcataaat gatctccaca gcttccacat gacctgtgta accaccactt acttgatcat
    83701 aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa aacagatagg atcccttttt
    83761 tcgattcaaa cggctcgacc atacaccaga aacatccccc tgcaaatact gcacgttctt
    83821 gatagtcagc atcttgcatg gcatgcttgg aaaagtcata tttttgctcc ttatcaaatc
    83881 cttccgtaat caataaataa aagtccatta catcaggcgt cagattcatt cggatagcta
    83941 atggacgtag tgttgcttct agttttgata actgttcttt aacggatata ttttctctta
    84001 tgtcattctt tgtctgaatc aatacttttc gttcccaatc tcgtgtattc ggattcaaaa
    84061 tcagattata aagtgtttga atgaatattt gttcctcatc catagattct cctcctttcc
    84121 gaagaagaca agctagtgac tttagctgta atttatcata aagtgaggaa ataagcgatt
    84181 acttcatccc cctacagatt tttcgtataa aaaagagaga atccttatcg acagattctc
    84241 ctttttaatc aatttatttt ataaatgaaa agttaattta ccttttatgg aaaactattt
    84301 taaaaaataa acataaatat gcagttaatg taacaagata agaaataata gtcgttactg
    84361 tataacttgt gcttacttta atatcagcaa ctcctttgac tccgcttatt tgtaccaatc
    84421 cattagcatt cacctgctcc tttacaggtt gatgatttat ttctacattc gttcctaaat
    84481 aagcatatac aggaagtgtg attacttggc tcttctcatt agttactcta tatatgatag
    84541 aactaccagt tgttttataa ggaaggttta cccattgact gccaatcttt gcttcgtgtt
    84601 ttattatatg ctccttattc tccaatgaag cgataggaga ataatcatat aaaaaataat
    84661 ccattccttt agtggtatac gcctctactg tttctttgtt tacaacaata tgtcccggct
    84721 gagaaattat tttttgattg aaattttttt tactagaaac agttaaagtg attaataaca
    84781 aaacagtcat aaataaataa aatagttctc ttgtcttatt gtatcttcta tttttgaaaa
    84841 atatcatcgt taatatcaga gaagagaata ttatttgcaa gcttaagatc cgccaaggaa
    84901 attgtaacaa tttggctggc gtattttgca gaagatacca tggcaccaaa ctagttgtag
    84961 ctagccaagt caaacacgta ccataataaa tctttttttc tatgtttgtt aattttttaa
    85021 ataaaatcag gggaatacat aaagataaca gaccaactag tcccattgag taagtagtta
    85081 agtcgttgtt catagcttca gtcagactat cgaacacatt agatgcatga gcatataaat
    85141 ttgtcacgct aggtttgttg attttttggt agagcatttg ttcaaacatc ggataccaaa
    85201 aagcactcgt cagaaccaat gtcattcctg ccgcactaaa taatttttta aatatagcaa
    85261 taaaatctgc attcttgaaa cctttagaaa ccaaacgaat aaaaataaaa atgataatta
    85321 atgctgtact cattaatgtt gatagtacat ggctgtacac tagcaaactc atactcaatg
    85381 ctagctttac agacggatac ttttcttctt ttccagagat gatttgataa gcataaaata
    85441 atacagggat aacaagtgtt attgcgatag cttcggcaat tgcggaacga tagtagatgt
    85501 ctactgtccg ataagcagag aaaatataga gacaactaaa taaaaatgcc gctttttttt
    85561 gttttaaaaa ccgctcgcca taatgataag ctacgagtgt tgtacacaag ttcaataacc
    85621 aaacgtaaat aatatagctt acaatcaaat tttttgtcat ccaatataac attacagctg
    85681 gaaaaaaagt cagaaaggga taaaaataat ttacaccata tccagtataa ttgaatgtct
    85741 cataattaat cggactggta aaaatagtcg ataacccctt gattcgagat aagtgaaaag
    85801 aaagatctcc agtggtataa atactctgtg tatttttgaa aaagatgaaa gaatatatac
    85861 tactaactat aattaatcca ctcaatatta ctactttttt atttattttc attcttcttg
    85921 tttatctcct ttttagtcac atatcgagaa tctttcaact tattaggtgc aaatcgttta
    85981 aaacttgaat acaccactaa acaataagtc agaatactga tccataaagc catgagagca
    86041 gttggagtat attttgttgt aatttctatc ttattttctc cagcatttac atccacattc
    86101 actaagccat gttttttata agagtcattt acgactttac cgtttacttt tacctgtgta
    86161 cctaaataag catatactgg tactgtcact ttttgctttt tgttgcttgc cactgtgtaa
    86221 gtaatgttat tatctgcagc tttatacttc cctttttccc acgattctcc tacttttact
    86281 tcatgttttt ctaagtgagg cctttctttt atcgcttctg ttggggcgta atcgtataat
    86341 cctcccatat ctgatgttgt ataacgtggg agtgtttctt cattgataac aatatgaccg
    86401 tgttttgatt ggataacttt tgcgtaattt tcttttgctg ctatagacgt gacgagtaga
    86461 aacaccacag tagcgccaat gattaaccat gaatattttt tattcatagt ctttttcata
    86521 aaaacaagcg ttatgatcat tgaagaaaaa aagatttgga tacctaagat acgccaagga
    86581 aactggagta agcttatcgg tgttttttga aacaaaaacc atggaaataa attagttacg
    86641 catacccatg tagaaactac gccaacatag atgatttttt cttttttctc catttctttc
    86701 agtatgaata aaggaaaaat caacgcaacc aatcctaata accccatagt gtatgttgta
    86761 atatcgttat ttaacgcttg aatgatacta tctgaaatag aaagtgcttc tagctgcaaa
    86821 tctgttttga agggactgtg tatttcttga taattcatct gctgcaacat aggataccaa
    86881 aaatagctag ttaatgcaca agtcaaaaaa acagccttta ctaaattcac gaccacaaat
    86941 tttatatcgt tttttatttg cttgcttaaa aacaaacgaa ctataatcat acaagcaatc
    87001 aatgcactac acaatagtgt agttaagaca tgagtataca caagtagact tatactgaca
    87061 gataaagcga tgactttttt ttctctattg aatatcaatc cataagcata atataaaaca
    87121 gctgggataa tcgtaagagc gattgcttct gcaattgcgg atcgataata gatatcaact
    87181 gttcgataag ttgcaaaagt atataaacaa ctaaataaaa aagcagcatc tatcttttta
    87241 aaaaactttt ctccataata aaaactaatc agaatcgtac aaatatttaa taaccaaata
    87301 tatagaacat aacttaatat taagttattt gaaattccat aaaaaacgac tgcaggaatt
    87361 attgtaagaa aaggataaaa atagttcagt ccatctccat aattgttaaa tgttgtatag
    87421 ttgattggcc cttcaaaaat agaagataac cctttgattc gtgaaagatg aaaagataaa
    87481 tcacctgttg tataaatttc ttgagcattt ctcataaaat ttactgaata taaagtacta
    87541 agtaagataa gaactactaa aagaaatgct tttttactta ttttcataga tttgttgttc
    87601 tcctttttta attctatttt cagtatacta gaattgtctt tttttaataa gttttgcgtt
    87661 aaatttttca tagcactagt atgatatact agaaaagaat caatttttat tacttttaaa
    87721 taggagaaaa aaatgaaaaa aaacaaaaac caaataatag atatcatttt tatgttattt
    87781 ctaagttttg cttacgtcat ccctctattc atgtctaagg gaatctatca tagtgtcaat
    87841 caggatactt actttcattt atctcgaatc ataggactag ataatgtatg gagttctcca
    87901 gtaaacttca ataacttcga tcatcatgga acaatgatga atatttttta tccatggctt
    87961 actctttatc cagctttttt attttataaa atgatgggaa atcttgtttt aagttataat
    88021 atctactatt tttttatcac ttttttaaca atggtcgttt cttatttttc tatgaaacaa
    88081 ataaaaaaca atcgttatat ttcgctgtta ttttcgatta tttatacatt ttccgcctat
    88141 cgtgccattg acatattcca tcgtgcttca ttgggagaag ctgtcgcttt aacatttttg
    88201 cctttgatac taatggggtg ttacgaaatt tatattagag attaccaaaa atggtactgg
    88261 ttgtctattg gcatgacgct agtagtgtat actcatttac tttctgtcgc tatggtttct
    88321 gtatttattg gaggaacgtt gtttctttct ttttattttt gggaccaaaa aatcgctaga
    88381 ctcttaagtc ttttaaaagc taccgtcctc accttttttt taagtgcggg attcttaatt
    88441 ccttttattc aacaatctag agcacaagaa ttgaaagtcc cactgggaaa agaattaagt
    88501 ggtatggctc ctagtgatat gctgacacat attttaaata ataactataa caactatact
    88561 attggattat tcttatttct cggattgatt ggtgctttta tttttataaa aaaattaact
    88621 gctgatgatt tatttatttt ctttcttggt gtatttgttc ttttttgttc aaccaattta
    88681 tttccttggc aactttttaa ccatacacca gttaagagtc tacaatttgt ctggcggtta
    88741 aatggttttt cttctttatt tatagcctat acgatgagta tagtgatcta ttatattttt
    88801 tctactaaaa ataattcatg gaaaataatg gtctttacgt ttcttgcact aatattgcat
    88861 ctctcaggag taagcaattc tatccatgct aacaaagatt cattaacact tatagcacct
    88921 gaagacgctg ttgctatagc tgaaaattac aatcatactg attatgcaaa caaagagtca
    88981 atctatcatc ctgatatgat taataatgat caatatttac taggtaacca ggaaataaat
    89041 ccaacaactc attttatgag taacaaactt acaattgaat tagataattc gaataataaa
    89101 aatactgtct tgacgacacc tatttatcgt tataaagggc aagtggcttc tattaatggc
    89161 aaactagtac aaacaaaatt atctaagttt ggcacaactg aattaactat tcctccaggt
    89221 ataaataaag tcgttattac ttatcagtac acaaaactag caattgcttc aagatattta
    89281 tctatcgtta ctttgatttt atttttactt tatcgattta ctttttctaa acaaaaacaa
    89341 ccgagaagag aagtacaaca ttctcactag atttttattt aagaaagact ttttagctgt
    89401 acacataaac aggctaaaaa gtcttttttt atatttcttt aatttgcttc atacattatt
    89461 taacatcgat atttatactg tgaaacatgc gtttcatcca ttatcaaagt atttttatcg
    89521 ataataaact tcatccaaaa acagaccttg tgcagggacg gtttctcctg catgttgccg
    89581 cactttgctt tcaaatattt catcgattgt ttctatcggc atggcacctg tgccgatttc
    89641 tagtaatgtc cccatgataa tccgcaccat tttatataag aatccgtctc cgacaaaagt
    89701 aaaatgaagc atttctccct ttcgttcgat agataattca tcaatcgtac gcactgtgga
    89761 tttttttgat tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca ctaatttttc
    89821 gcaagctgca ttcagcttgt ccatatccaa ctcttgaggg aaataaaagc tgtggtttcg
    89881 ctcaaatgct gaaggaatca cgttgttcca aacataatag ctgtattttt tgcccaccgc
    89941 attatatctt gaatggaagc gctcaggcat ttcttcgatt cttttgatga caatatcccg
    90001 cggaaggtaa cgattcataa agtcgatcat ttctgccagt ccaatttctg tgtttgtttt
    90061 aaaatttgct acttgtcctc gcgcatgggt gccagcatct gttcttcctg atccaataat
    90121 ttctatctct tcatttgtca tctgatgcaa tactttttcg atttttcctt gaatggtttt
    90181 atcgtcatct ccaagtcttt gccatcctga atatcttttt ccgtcatatt cgattgtcaa
    90241 cttgatgttt ctcatatttt agtggctcct ctgtcttcca tttggtcttc tctctacctt
    90301 tttaagattg tagcacaaaa tatagctcct tgcttctcta agacagaagc tatacaagaa
    90361 aactgtggaa atatatattt cagaaaaaca aaaaacagcc accggatgaa gtatccgctg
    90421 actgttcaat ttgacggctc aagttttact gtttgagatg caagatactc aacctggcaa
    90481 tgggcacata attaaagaaa aaactgattc tttggcaaaa acctatttta ttcagtaatg
    90541 gccaagaatt tatagaagga attcttgcca gtccataaat cgccgcaaaa agcaaactga
    90601 tcacagacag tccaaggtat aagtggctta cgtccatttg cccatttttt cgatagatca
    90661 agaaaagaag aactggaaac aataaaaaac cactgtaacc taaacctgat ccgatattat
    90721 ggacccatgt cgagacattc cacgaagatt catgggtatt gatactgaac aaacctgtaa
    90781 agatacaatc acctatacca tagaacgcga tggctgctgt aactagggta gctaacacac
    90841 gagatgtact aacaaatgca gcatacaatg caggcaatga caggatgaaa aagatccctg
    90901 aaaccacaga ccaaataaga aacgcttttc ttacaggact ggaaacctct ccaaatacac
    90961 tgatcacgct cttcatttga tctaattcag gataatacaa tcctaatacg tacggagtca
    91021 aaaaatcact cagcacgcct aataataaaa agaaaaaacc aaatttacgt aaaaaatcca
    91081 tgatacgcct ccaataacaa ttacctatgc ttttaattgt tattataaac gactttatct
    91141 tgatttaaca gaaacctcta tttacacgaa catctatata actcattaaa aactcgaatc
    91201 attttaaaaa tactaaatat ctaacgttca acgttcactt aatccatcta aacaatcttt
    91261 caatttgatt ttttccccac ttcttttacg cgcgcctagc tctcctattg cagtaaacaa
    91321 cagatcactt gacgaattta aggcagtttc cattgaatcc tggataacac cgataataaa
    91381 tccaataccg acgatttgca tcgcgacatc attagagata ccaaacaaac tacatgctaa
    91441 tggaatcaat aacagtgaac cgcccgcaac acctgaagct ccacaagcag ctaaagcaga
    91501 caaaatacat aaaaggactt tcaaaacaaa aggtgcttca atccctaaag aatgtacagc
    91561 agtcaacgtc atcaaggaaa tggtaatcgc ggcaccgccc atattgatcg tagcacctaa
    91621 cgggatcgat atggaataag actcttttgt caatttcaat ctttcagcca acatcatgtt
    91681 tatcgggata tttgcggcag aacttcgggt aaagaaagcg ggaagtgcgc tttctttcaa
    91741 acaaaagaaa atcaatggat agggattttc tttaataaac aaaaacgtga ggaatgggta
    91801 cacaactaat gcgacaaaca gcatcgtacc gatcaaaaca agtaaaagct gcagatactc
    91861 tgccaatcca gcgattccag tcgtagcaat tgattgataa accaatccta aaatccctat
    91921 cggagcaaat gcaataatca cttgtacgac ttgtgacaac gctatagata tctgatccac
    91981 caactctttt gtcgattctg aacgtgctcg aaaagctagt cccaacaata cagcccagaa
    92041 taagacgcca aggtaattcg catcaataag agattgaaca ggattttgtg tgacattcgt
    92101 caacatagta gataaaacac tttccaaact ttgcggtgct ttttcagata ctgtttcttg
    92161 taaagtaata ggaacagtaa aaattgaact tacaataact gctacaagtg cagaaagcaa
    92221 tgttcccact aaatataaga tcaaaatagg cttcacgaat gtcttattcc caattttatg
    92281 cttggcaatt gacgccatcg tcaagaaaaa aaccagaata ggtgcgattg ccttcaagca
    92341 actgacaaac aacgttccca atattgaaat aaatgtccat gtggggaaaa agataccaaa
    92401 caatattccg actaaaatac caacaacaat acgttgaact aacgacatat ccaaaaactt
    92461 ctctaacaaa tgaccaccct ttcacaattt taacaatcat tgtatcattt tatgcaaaga
    92521 aactaaaggt ataattttct aaaaattcta acaatacaaa caaccatttc ttgctaattt
    92581 tgtacttttt ctgtctacac gttgtttttt gagatattct aagttaggat gttattaatg
    92641 ggcaacttat caggaaacgt tttcttatgt ttttgtccaa tttttttgtg aggtgtttat
    92701 tcttgaaaaa aataaagaag gagagttatg gcttcctgac aatctttgtc gcttgtataa
    92761 tgatggtttt acagcgtata ttgatttcag cagtcactat tcctacatat gcaacaaacc
    92821 ctggtcgatt ctacatctat acatttttac aaacttccgt tgttattggt gcaaatttga
    92881 tcccacttta catgggttac caatatcaaa aaatcgcccg tttaaaagtt ttacattatt
    92941 taagtcgttt tgcctttata ttcctaatag ccacactagt ttgcaatatc tttttttacg
    93001 tacaagctgg cagcttgaat attcgtgatt attggcttat cctaacacct atcagtcaga
    93061 attactttac ttatgctgtt tcttgtactt tgcttttttg tagtattcct tatacagttg
    93121 gatttttaaa taaattatcg aaaacaacaa ttaaatatct actattattt ttaacaattg
    93181 gattagttgg ctgctctact ctatttaata aagacccgtt tgcattacag aatggacaga
    93241 gtattttatg gattttttat ttatttttaa tgggttatgg tttgaaagaa tggaactgga
    93301 aagaaaaggt ccgctataag cttcctcaag tatttatcag cttgattgtt ttattcggtt
    93361 tgattatttt gatgacacaa atttcactca tcatcagagg aaatacagat acagcgctga
    93421 ggttttctaa accatattct ctcttcgcaa tgtattatac tttctcttcg tttttatttt
    93481 tagaaatgct atctgataaa ttaggtataa aaggaagagg aaattttctt gctgtatttt
    93541 tgattgtcac tcaagttata tgtaatttcc ctttagtttc ttatcgcgta tctacattta
    93601 tgaaacgtga atttccaaac tcagggaagg catggctgat aaatatcggg cagtttttta
    93661 tttgatattt attagcagtg tccctcctag ttatcttttt attttatata caaaaaatcc
    93721 gtttcattca tcgtttcatc gaatacttcg cttttgattc gattgaacaa ttaactcagc
    93781 gttggaaaaa aagaattcat tggctttttg ttcataaaag ttatttttta gttgcagcat
    93841 ttttttactg ctttaccttc gtgcagattt tcgttttaga accaaaagaa ggttggaaag
    93901 aaactatcca ggttgcctta aaaatattta ctcagcgaca agcacccatg atcctgacaa
    93961 tcatcattat tatgggattt ttcttcttac tattgttgat ttccaatcgt ttttggtacg
    94021 ccttgactgc taccttgatc attaacttac tgctcactat ttcgacagta atcaaaatgg
    94081 aaatgcggga agagcctgtc tttccttcag atttaaaaat gctgaccgga ttgtcagaac
    94141 tattatcgat ggttagccca gtcttattaa tcgtgggcgg attgatcctt ctcctattat
    94201 tgattactag tattattgta caacgtagac tacaaaaaca gtattccttg aagattcatt
    94261 ggaaacgtcg atttatcagt atcgctgtat tattaggatg tttttcgggt gtctttttca
    94321 ttaatcacaa aaattcgcct tctttcttgc tgtttaattt atttaaagta aacaagactt
    94381 tctttaacca gcaaggcgcc gtaaaagata acggaccagt cattcaattc ctaaacaata
    94441 tcgacattaa aatcatggaa gaacctgctg gctattcgaa agaaaaaatt aatggaataa
    94501 tgaaaaagta caacacaaag gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca
    94561 atcagacgat cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc
    94621 tgacagtacc tacagaccct attccttaca tcaaaagcat tataacagaa ggaacaggag
    94681 gattgatgtt atccgtcggt tatggtggcg gaacagctaa catggaatgg gaaggactga
    94741 caggcttgag tatcagtaac ctgtctgctt cgttagttac accttatacc cagctagtgg
    94801 agcgtcagaa aataagtcca aatattaccg atctattcga tgaaaaaata gccatccatc
    94861 cttttacagc tgctctttat cgcagaaaac aagtgtttga taaatttggt tttgacaggt
    94921 tctattattt agacagtccg gataaattaa cttatacgga taaaatagaa caaagcccac
    94981 gcatatcgga tgattcagca tacaaagaaa ccttgaaagc tttacaaagc aatacggaaa
    95041 cgtcacaatt catccaatta tccactatgc aaaaccatat gccttatgat gattactata
    95101 gtgaattgaa ctacactgct gaaggaactg ctgtcattcc taatagaaga aaagaattac
    95161 aaacttatat gcaaggcctt cactataccg atacagcagt aaaaaaattc atccaagaaa
    95221 tcgacaaaat cgataaaccg attacctttg ttttttatgg tgaccattta cccgcaatct
    95281 atagtgggaa tgattcgaaa aaatatggat tagaacagca ccagacagat tatttcattt
    95341 ataacaacaa gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt gttgctccta
    95401 ataactttgg tgctatggct cttgagcatg cgaatatcaa agtaactcct tactatgcgt
    95461 tgttgacaga agtatctaat catctacctg cattaacaat tgatcctaca gaaagcttgt
    95521 ctaatcgttt taatggaaaa caggtctttg ttacagagga tagtcaaatt atccaagaaa
    95581 aaaatttaac tgagaaacaa aaagaaattc tagaagatta tcgtctgata caatatgatc
    95641 ttgttgcagg taaccagtac agtgcaacat gggcgacaca aaaaataaaa gactaacaaa
    95701 taaactgagc aaggggttgt gataaaagac atgtcacaac cccttgttta tctcattaat
    95761 ttgcttgtcc ataaacgatt tgattaaagc catttttcaa ttcattattc gctgtctcgt
    95821 tgaagtaaag ataattatcg ggattcccct gattttgttg tgtcaccaat acttttggct
    95881 gattttgctg aaacccgaat agatagacgt gctttttcag atatggttct gtctctgcat
    95941 cggaatagac ggctgtgatt tgatagtctg cttgacctgt cccactttct gaccattctg
    96001 cagaaacagg cactccacca atagccatct tccactcccc gttcaaaata gcttgaggga
    96061 ctttaagacc atataagtcc acactcattt gattcgtata acttttgtat tgttgtccca
    96121 ttgtttggcc ccatttggtc ataaatgcct ctaattgtgc ggatttgtct gcattccaca
    96181 gtgtttgttc aacttgttcg ctgctttctg aagaggaaga tgaagcctct acagaagaac
    96241 ttgaagtaga tgtttgagtt gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat
    96301 ctgctgtttc tgtacttgct gcagttgact tgctctctgt gctagttgtt gtagtagttg
    96361 caactgttgc tttcgctgtt tcttttgttg attctttttt cgattcattc ccgttggtac
    96421 aaccagctaa caaaaaagct agaccaattg ttactaaaat cctttttttc aattaaaaaa
    96481 ctcctttcga ttactcccac ttctttattc tatcaaataa gcaatgtgat tttattgatt
    96541 tttcgtattt tctcatcaat ctttcaaatc ttctaaaaac tgagaagcac ccgctgatgt
    96601 tgtctgtaat acatcagcgg gtgcttcttt tattttaatc ttcgtgattt tccaatttac
    96661 ctcacgcttt atatttgtat ttgaagaaac atagactttt agtcatcatc gtcttcctca
    96721 tccaaatctt gtcggacagt ttttccttgg agtctttgta cttggtagat gatttcttta
    96781 ttgaaactta aaagttcaac aagcaattgt tgcaagccat atttttcttc tttttcagct
    96841 aatttgattg cccgtgtgat aaagacattt tggaaatcaa aatcttttgc aaagttttcc
    96901 aacattgttg aggctggtaa atatttgttt tgaccatgtt cgtaaatttt tccataacgc
    96961 atgacttctt ctaaagtaga cggaacgatt tcattttcaa tcactaactc atgagagatt
    97021 ttattgaacc atctcgcttc ttgatcagca tattctgaaa aaagggtact taattcttgc
    97081 gaagatgtac ccaaaacata caattgtgct tggatcaatt tcatttcttc taatttaaaa
    97141 tttgctaata catgccccac catggcgcca gcagttggtt tatgatggtc gatatctgct
    97201 tgttttgttt cttgaacaaa tttttcttcc actatattca ttgatttacg cctcttttac
    97261 ttttgctgat tggacttcat aacctaattt ttccacaact gcagctaatt gttcagggtg
    97321 gacttcatct ggatcaaagt ttgctttgat tttgctcgca ttaaacaaaa ctttcgtatt
    97381 gcttacccca ggctgttgag aaaccgcatg ttcgattttt tgcatacagc ttggacaagc
    97441 tagtgtatct aattgcataa taattttttt catcgtagtt cttccttcct ttttcttgat
    97501 agtcttattc tagattacag cttccataaa aaaattgatt cttatcaagt tttacgattt
    97561 ttcttctttg ctttcgtgtc caaaatacaa taaacgcatc gcgttaaaga taacgatcaa
    97621 gatactagct tcgtgaacga acatcccact agccatgtaa ataaacccgg caaataagcc
    97681 gttgagtaaa aataagacga caccaatggc aatcactaca ttttcacggg tatttaaaac
    97741 agttttctta gccaagcggt aggcatgaac caacgattca aaattagatt gcatcaagac
    97801 aacgtcactc gtttcgatag caacatccgt tcctgatccc atagcaatcc cgatatcagc
    97861 cgcagccaaa gagggagaat cattgattcc atctccaata aaagcaacgc gcaatccttg
    97921 ttcttggtat tttttcacaa attgaacctt ttcgtctggt aataattcag catggacttc
    97981 atcaatgcct aacatatcag ctacataatc agcagtcatc tgattatcac ctgtcagcat
    98041 cactaactgt tttgctcctg cttgtttcaa cttagctaat tgttcagcga cttctggtcg
    98101 aatcacatct gacacaccta tgatttgagt gatttttttg tcgattgcta caataatgac
    98161 cgagctgcct gtttgttgta attgatgtaa atcttgtact tgctttggtg tgagagaaag
    98221 tttgtgagcg gcaatcaatt tggtgttgcc cgcacaaact gtatgaccat caatctcagc
    98281 tgtgattcct tgtcccttga ttgtctgatt atctttgact gctaattgtt gaaaatcaat
    98341 gtctttctcc tgagcataat ggatcacggc gcgccccaga ggatgatcgg ataacgtttc
    98401 taatcgagcc actaaagcta agctgtcccc ttcatcctct gtatagttct tgatagtgga
    98461 tacagcagta gttcctttgg tcaatgtacc agttttatca aagacaaaag tatcgatttt
    98521 ggaaaaagta tccattgctt caccgccttt gactaagatg ccccgtttcg ctccattacc
    98581 gattcctgct acattcgaaa caggcgcacc aatgactaaa gcgccaggac aaccaagaac
    98641 caaaacagtg attgctaaac gaaaatcacg actaatcaga ccaacaacca acgcaataag
    98701 taaaacggtt ggagtataat actgagaaaa tcgatcaatg aatttttccg catgggattt
    98761 ggtatcttgt gcttcttcta ctaattcgat aattttggca aaggtcgtat catctccgac
    98821 cttagttgct tcaatcgtca cgtagccatc tgctaaaatc gttccagaga agacttcatc
    98881 tccactgatt ttgacgactt gtcgtgattc accagtcact gcggcttcat ccgcataccc
    98941 tttcccgtca atgactcgtc cgtccacagg aatagctcca ccagttttta ctaagacgat
    99001 atcgccttct tccacgtcat cgatttccac ttcttcagtc gttccatctt cttggacaac
    99061 actagcagta gatggggcca tctcggtcaa agccttgatt gactcccgcg ttttggtcaa
    99121 tgttttttgt tccagatagc taccaaacaa aaacaagaat gtaacgatag ctgactcctt
    99181 gtattctccg atcataaagg caccaagcac agcaatagta acgagtaaat caattgagat
    99241 aactttgact ttcactgcct gatacgcatg gatcataata ggtatgaccg caataataga
    99301 agctatggct agagcaatgt tatagaacaa gacaaaacca gtaaattttc cgataaaacc
    99361 aaaaataatc aacacagctg ataaggcggt gatatgattt cgatgctctt ggatccactt
    99421 ttgtaatttc atctttctcc ctcttttcct ttcattctta cgagactaag tatagatact
    99481 tatccatcat aaaaaattga ccactatcaa gtttagtcaa aaaggcggtt ttcttttggt
    99541 agaatacact acaaaaaata gttagcaaga agactcacta aaagtgatct tgccaactat
    99601 tgtcgtttct atcgaaattt tcattgaatg attttcctgt acagcataca tcggagatct
    99661 atttctttat ctaagatttt tatcttgcta attttttcct ttagtctttc aaatccacgt
    99721 cgttcataga attgatacgt acagccacta tctgtaaaaa ggaatatttc ttttcctttc
    99781 tctctttttt caaattctgt tagcaacttg gtaccgatgc cttttgtttt caaatcagga
    99841 tttgcagcta aaaaaatgat ctctccatct ggcgaatgct tctttaaata tgctgcaagc
    99901 atttgttcat ttgctgtctc gtaagctcct ttactttctt tagcaaatat attttgtaaa
    99961 aaatcgaata tcttaacata tatttgctgt ccaaaagaac gatgtttctt tttttctcct
   100021 tttatctccg ccagcaaaac acctgccaac tcgtcctctg catacaaagc aagaatttga
   100081 gttgctcgat tcaactccat atacaaaaaa tatcttccgt aaagattcag cagaagttca
   100141 ttttccatat atctctcaaa gttcattcct tcaacagcaa aatagatagc ttttttgtaa
   100201 tctttctttt ctaagggttt aaaagttagt ttcatcattt cgcctcctca acttcaatga
   100261 ttgtgttata cttacattat aatagacaca gtgtctacta tctataatca atcatctata
   100321 agtttgtcga ttaagcgaca gctttgcttt gatatgtcgg ataaggagat aggtaaatga
   100381 aaaagacaga tagacgcgtc aaaaaaacag aaaaagcttt ggctgaaaca ctatccacat
   100441 tattagtaaa taaaaagatt caagcaatca ctatacgcga actaacagaa acggcagatg
   100501 tacatcgttc gactttttat acacattata aggatatcta tgatctatat gatcaattag
   100561 aaagcaactt ttttcgggat ctaaatgaaa ttctctctta tgatccagcc catagctatg
   100621 aggaactata cactcgcttg attgactatg tttacgcaaa tactttttta tatcgcttat
   100681 ttatcctcaa ccatggtgat atttcgtttc agaagaaagt taaacaagtt attgaagaaa
   100741 actatctgaa catatggcta tatgaagaca agaaaacgat tatcacagaa gaaatgagat
   100801 tctttactac gtaccacgtc caaggttgtc tatccgttct caatcggtgg gtagaaagca
   100861 attacaagta ctccaaaaaa gaattattag aactcttaca gaaactgaat gatcacatag
   100921 agaaaatcat gccttgaata aaaacatact aagcttagtt aagaagaacc gtaaccttct
   100981 cgtataacaa ctgaatctta tttagccaaa ttgtttactg aagatttttt gagtaaaatt
   101041 tctataattc ctacttattg aaaagtagtt tgtagtgctg cttctccctc tctaagtgat
   101101 atatcttgtt gtaccaatca aattcacctc atcattaata aattactttt aggctaattt
   101161 attacaactt ataggttgaa gctgtaacca atttatagtc atattatatt tttatcatcc
   101221 agtttattga attcttctcg tttacctcaa ttcattttca aggggaatca gctcatcatc
   101281 taacatttta cgccatgctc gcttcatttc ttgtgggtat ttaaatggtt tcgcttctgg
   101341 atgtttggct ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat
   101401 tggatcttca cctggcatat attgcagatt agaagcatgt aattttttct cagaaacaaa
   101461 ccactcattg gaaaagacat gaatcaaatc atcacttact tgtgtaaaat agcgacgttt
   101521 aatgacaaag atatcttcat tgctatctag tgtaccttca tttctttgtg cttaaattca
   101581 aagataattt tctcaataaa gatttttgat tccagtaaga ttcctttact ccttttacaa
   101641 catagcgttg tcctttgttt tgaaacatct tcttcccctc tttgccatta aaaatagccc
   101701 aattctctat aaagaagatt aagcttcatt attataatat aaagtttaaa tttggtttat
   101761 tacttacttt ttcttattct ctagtaagct aatattgaaa aaaatgtaat cagatcatta
   101821 tttttatatg ttgtaacttc ctgataagaa aactaattaa aagaaagagg taactacata
   101881 aactaattaa aaaagctaac ttcatagttg gaggataaat ggaagaaact tcaatgattg
   101941 tttctccttg tgttccttca atcgacacta gtcctattat caatctctct tttttgtcca
   102001 tttacttttg cgactgttcc ccaataatca aatacaggaa gcgttatttt ttgtccttta
   102061 gattcatgta ctgtatagac tattttcgaa tccatcactt gataaggcat agtatgccat
   102121 tccttgccaa ttcttacttc atgattctgc aaatgttctt tgtattgtaa tgcttccgtg
   102181 ggtgcatagt cataaagtcc tccgggaagg agtgtcgtgt aacgaataag attttcttga
   102241 ttcattacca agtgatctct ctgagtgaca actttatgtt catagtttgc ttttgttgca
   102301 gcagatacag ccaacagtaa taaaacaatt gccgttatat taactacttt tctttgctta
   102361 gctgatgtgc tcttcatgaa aacgaacgat aaaatcaacg agccaaacag tacttgaaag
   102421 cctaagattc gccaaggaaa ttgaatcatt tgaatgggtg tattttgtaa taaagaccat
   102481 ggaaaaaatt tggtggataa gagccaagag actaccgcac taaggtaaat cactttttct
   102541 ttgcgattaa atcctttaaa aaagaagagt ggacagatca aagaaataat accaatgatt
   102601 cctaccgtaa aggttgtcag gtcattattc atcgcaccaa tcaaactatc cgagacattt
   102661 aatgcttggt gttgtaaggt cgttttatct ggctcattaa tcgattgaac catgatttgt
   102721 cggaacatcg gataccaaaa gtatgctgtt aaaagaatcg tcattcctac tgcctttgac
   102781 aactccacaa tcaaccggat gctttctttt tttcttgttt cacttactac aaagccgcct
   102841 aagactagta agccaacggc tattacactc ataaaagtgg aaagaacatg tgtataaatg
   102901 agtaagctca tactgactgc taatggaatt gcacatggct ttttctcata aatcagtgcg
   102961 taagcataat acataactat cggtataacc gtaagtgcaa tcgcttcagc aatagcagaa
   103021 cgatgataaa tatcaatcgt acggtaccca taaaatgtat ataaacagct aaatataaaa
   103081 gccgcatcga ttctttttaa aaattttagt ccataatgaa acatcagcat gatagtacaa
   103141 acattcaata gccaaacata taagatataa ctaacgatta aattattact tatccaataa
   103201 aaaatgactg ccggaaaaaa ggtcaagtag ggataaaaat aattcactcc gctcccataa
   103261 tgattaaacg tcgtaaaatt gattggacca gaaaaaattg aagataaccc tttgactcta
   103321 gcaaaatgga aggcaatatc ccccgatgta gaaatagtat gaacatttct aaaataattg
   103381 attgaataaa gtccagcaat aattgctagg agaatggaca atattatttt tttctttact
   103441 tgcattactt aattatatac tcctttcatg gattgcttta tctagtataa cttaaatttt
   103501 aaataaatcg ttatttattt ttactctcac ttaaaaaagc actaaaatta taagagaggg
   103561 aggtaatggt ctgaacacca agaattgttc caacatcgaa ttgcttaact aatgagttca
   103621 aagcttaatt atgtggctct tatatttacc gagcgtggat tgcgatctct ttagcgaaac
   103681 aaacatcttt cataaaaaaa acagccatat ccgctttctt ggatatgact atttactact
   103741 actttactat ctttcattct tcttgtctca cttatgataa ggcgctgcgt atcatgagtg
   103801 aatgatatta ggaaaacttc ataaaataaa tcttttatac atttccactt ctagatggtc
   103861 attcatgtta gaagaatcaa actatttttt tactgtcgta ataatattaa aacactagaa
   103921 caaatcaatt aaattccata aaatataaat aatagaccta gcatcatctg gtataaaatg
   103981 gatgctaggt ccgttatatt tactcaaaat tgattctacg aatagatctt cttatcattt
   104041 ctattttagt agtcatgcgt tgttgttctt ctaaaggtgg taatggtata agtagtttag
   104101 ataaatttgt accatttaca tttggttgcc cagttccgta agaaattttt tctaataact
   104161 tccaatataa tggcgagtct aagaagcagt ctacgtattc tgataataat ttctcaacta
   104221 acctaattcg aatcaagtaa gaagcaaaaa cagattcctc acttatttcc ttaaccaaga
   104281 aactttttcc catagttcct cctgtccttg ctatcaaaat gtcattttct tccaatctta
   104341 aatcaactag tttagaatta ctaatatcac agtaaggcac actggaccaa tttacacgtc
   104401 cctcttgtat atctgtgatt ctcaaaaact taacattgcc ttgttttttc gccgaatctg
   104461 tatagccata ttgaatctta gtagaaatac ttcctaaata aacccatagt cgaacatcgc
   104521 tagatgattt ttcttgtcgt tctttatgaa caacagtgat catcttgagt tgtccgacta
   104581 ttttttagtt gcagcaatct ttttgggacg cttatttgca tgaagcaacg ttgctaagtc
   104641 tagtaataaa acattggcta acacaggatt ggctttctcg cgatacgctg cggcgccact
   104701 ttcaaacaat actgctagac gatcttctac aacaacgatt tgttccaaat acggtggaaa
   104761 tttgattcgt tttaggatcc ggctaggaga gtcccgctcg gacactaaga gttccgaggt
   104821 ataaggacca taagaacgac tggctaagag ataatggcca taaaaagcca caccttgaat
   104881 cttatcagat gtttttctgc tcattgtcac ggttggttgg attttgtctt tgcggcgaat
   104941 tgctgttttc atttcagcta tcgtcggcaa tagatagaaa gtcaactgtc cttcaccttt
   105001 tagtgtgaag ttgccagata ccagttgatc attgttttga gtcaaatacg atgcttgtgg
   105061 aatctctgag agatcagttg tcagccaata agcgattggc tgtcccgtct cttcagatgt
   105121 atctgctaag atatcgatca aacgtaaagc ggctactcgt ccagtagcag atcctgtgat
   105181 tgtcaaccat aaaagttccc gttttgtatc atatactaag ccccctacat gtggccgttt
   105241 aggtaaaatc agcagtttga tcggctgtcc tgtttctcta tccagtacat gaataataga
   105301 atgatgctga tggtcatagc agtaggcaga aataaacaca aaatcttgag aaaaagccaa
   105361 cccttgcggc gtcatccatt gacaaacctc attttcgccg gtttccggca ccaccattct
   105421 tgtttctgtt aatccaggaa tgttatattg ttttgcttca gccttttccc aagctgcttg
   105481 aacaatagga taccgtttta actttttttc aatatcctga tgtatccgtt gctgcttcca
   105541 atgcttaaac tttaggacat agtaaacaag aaggccaatg accagcaaca ctaaaatcat
   105601 tattgctacc cgctgtgtca tttttttgcc tccttgttct attcgatcaa tcgtccatat
   105661 accgctatta attaccgtaa ctacatgttt cttttactag cgtttcttta taggatcttt
   105721 cataatctca tttatagtaa cattaagaat cccttattct actatagtca cttccacatc
   105781 aatattgcct cttgttgctt tagagtatgg gcaaaatgcg tgggcttctt ctgcgatttt
   105841 ctctgcttgg tccttctcaa gtcctggcaa agaaacttct aaaacgaccc cgatttggta
   105901 gcccccatct tctaattgaa ataaagatac ccgtgctttg acttcagaat cagactcaag
   105961 attattttct tccagcatgt gttgaaccgc gccgttgaag caagatgcgt atccagccgc
   106021 aaaaagttgt tccggatttg tcccttcact atggattcct gggcggtcga tcttcatgtg
   106081 catacttccg tttggtgcct caacttcgcc atcacggcct ccacgattga tcattgctgt
   106141 ttcgtacatt tttttcattg agatcatcct ttctttcgtc gttctatatt aagtgtaacg
   106201 agtatttttt aatctgacaa aggtaatgct tgcattcggc gacatcagtt agacaacatc
   106261 gatgatcagt gatagaagca gtttatctat acaaaaaaag aagggttcta tgatatttgg
   106321 tgtccttact cgccttgagt aacaacacca aatatcatag aaccacttaa tcagcttcac
   106381 actgcttttt ctactttcgt agttgaaaaa aacttaaaca aatcatcggc aaatagttaa
   106441 agaaaaaact taatcgttga aagaagccca attgcttaaa gaaggcaaac tgactaatcg
   106501 acggaatccg tgctaatccg taaagacctg caattagtaa actaataact gttaaaacaa
   106561 gataaaaatg gctaagcgta ccttgaccac tttgacgata aagcagaaca agtaacaatg
   106621 ggaaaagtag aaagcctgca tagcctagcc cagaacctat attatggatc caagtcgata
   106681 gattacaaga gctttcattc gtattgatac tgaataagcc agtaaaaata caatcaccta
   106741 tcccatataa accaactgtt gcaacaacaa gtattgcaag tgtttttgat gttccaacaa
   106801 ataggtgata caacgcaggc aacgaaagaa caaaaaaaag tccagataca actgaccaga
   106861 ccaagaaagc acgtctgact ggactatcta cgtcaccaaa cacactaatt acggtagtca
   106921 tttgattcag tttgggataa aaaaggccaa ggatatacgg tgtcgataaa tcactcatca
   106981 cacctaataa taaaaaataa aaaccatatt tttgcaaaaa aatcattttc ttcccctact
   107041 ttttagtgat tattcgatac atccaacaat caaatttttt ctcattttaa cacatatttt
   107101 tattctagtt gtcttttgaa caatcaataa cataaaggtg agtttcttct acattttaaa
   107161 aaatcgaatg gcactgaaga gttcagttgt cagcatcaat ctcctactca aaaaggcaaa
   107221 aaaacgtaag tcagatgttg ttgacatctc acttacgcat ccattgattg aaaatttctt
   107281 agtaaaatct actagcctaa aaatttgaag acttcttctc gatcatacag tttaaatact
   107341 ttctcgcata aaaattgagt aattagtgcc gcaaaaacag ggatgactaa ccaacaaaga
   107401 acaacaagaa agccatttag ccctgcatct aaagaagcca acggaccaac aagtccgacc
   107461 acgccgaaac cagcagaagc aggcgtacct gaaatagaaa atagagcgac aggaatagct
   107521 gtaatcactg cactaactaa acaaggaata agaatcacag gttttctaaa tagattaggc
   107581 atcatcatct tcatagcgcc taaagcaatc gcaattgtca cacctgactt attaaccttc
   107641 caagagttca ctactaaaac aaaagtggtt gcagctacgc ccatcgcagc cgcaccagca
   107701 gaaacaccat ctagttggat tgccattccg attgctactg ttgaaatagg agagataatc
   107761 aaaaaggcaa aggaacaacc aatcaagata gacattaaca gcggttgtaa ggtagtgaag
   107821 ttattaatca atgatccaat tgcagaagta attgctgtta caaaagggaa taaataaaaa
   107881 ccaagtaaac ctgcacctac acctacaaca atcggtgtta acacaatcga cactgaccca
   107941 aaactatttc caatcagtaa aatcagcccg acagcaatcg atgctgtcag catcgtatta
   108001 ataatatcgc ctgtaccagc accaacgtaa acacccgttt cacccaaatc aggtgtgaat
   108061 ttgactacac cagaaccaac aaaagaagct ccggctacaa ccatcattct catcggatca
   108121 aatccaaatt gtaaggcaat caaaccacca atgatcaaag gtgttgctag ttgaaaaata
   108181 actgctaact gtgcgatcgt taccgcaaag ggatacgcac taaaatattt taaaatggca
   108241 cctaatactg cattaggaat caatccaata atgattcctg ttgctgtacc agcgaggact
   108301 ttattcagaa aaattttcgg tgatattttt tgtgaagttt ccattcaatc tctcctttat
   108361 tttacgttca aactatcttc ttttgcatgg acaagctcag tcaagaaatc acaataaggc
   108421 gtcgcaacac catatttctt cccttttcta gaaattgcgc cattgatata atcgatttct
   108481 gttaatcgat ggttcttaat caagtcttga tacattgaag gataatgtaa cccaattgtt
   108541 tccgggtcaa aacaagattc acaatgtgca atgacttctg ggacatctag ttcaatcttc
   108601 tctactgctg ctactttggc aaactcattg acaatcgtcg ccaccatttt atgagcagtc
   108661 gatgtttttc ctagctcagc catattaacg tctaagattg tgcatagtcc gttcattgtt
   108721 ccgttcacac atgctttgcg ataaatagaa tagtgaatat tgtcagaaaa atgagcattt
   108781 aatcctgatt cagacaattt gtctgctaat ttttttgcag ctgcttcttt tccatcacct
   108841 aaattttgta attctactga accacttcca aataatttga cctgaccagg accttcaaga
   108901 cctgccgtcc acatggtatt tccaatatag atattttcca ttggtacaaa tttctcaata
   108961 atatcttcat gcccgatacc atttaataga cataagacct ctgtatcttt tttgattaat
   109021 gattggatat cctgcagcat tttttccagc tgcatcgctt tggtaaatag aataatcaga
   109081 tcaacttgat cttctttttc tacttcggat tgaagaacga ttggtagttt tgcttctacc
   109141 tctttaccat taaaatttgc ttgtaatcca tgttctttga tttgttggac atgttctgcc
   109201 cagccatcga ttaataatac ttcgttccca ctttggtgaa gcatcaaccc aaagcgactt
   109261 cccattgcgc cagcgcctgc aattgctatt ttcattgatt cttcctcctt tttctttcta
   109321 ttatactcat ttgagaatct tttcacaacc tcgttttgtt attcttctct tatttatcat
   109381 gtttatatcc accattcaag ttattttatc agcaaatcaa taaaaactgg aatagaatcc
   109441 caatttcatc gtaagagaaa ctagactagc aataagaaaa tagaaacatt catagaaaaa
   109501 atctcactcc tctgattata aaagaggggg cattaacctc gtgtatacta aaaccttcca
   109561 ttcattcgat ggatgatttt ctttttgtga aaaaatattc attgtagtac gatgttgcgc
   109621 aacacagacg gatggtattg cttattattt attaaaatcg aacaatacac ttatttaaaa
   109681 acttctttcc cagattttct tccattttta cagttataac tagtattatc atgaaaaata
   109741 gccttttatt gtctatggga ataataattt aaaattggta cttttttatt aattagttca
   109801 tcgcccaata atctctccag tttcttagca tctcctgact caaccggaat tatccaagcc
   109861 cagtctaaat aattttttaa aatctcatca tattttgcaa atatttcatt gccatattcc
   109921 gtccttaaaa ttatattttc agtgccagac aagtgattta tattgagttg ataaaaaaga
   109981 tctccatcta aaaaaatatc attagaagaa gatttcccca cagtaatgac cattccatca
   110041 gctctatgtg ttacatacaa ataattgtat gacctcattt cacctaatga atcaattatt
   110101 tcttgccctt tagctctaat atattgatct ctattaccta tctcactctt atttaacaaa
   110161 agttttttca tgtcaaattt aatcggctgt tttatttttg taaattcgct tctactacta
   110221 atccaatccg agattaattt ttcaacatgt cccagagtaa actctgattt attcgctaaa
   110281 tacttttttt gttgtattaa agacatcttt ccccgaagct caaaattgag cctacatcga
   110341 ttctctatct cttcaaaaag catcttatct tcatcattct tatattcatc attcataaaa
   110401 ccaatccagc tcctcgatta agataatatt ttaatctcat acaaaaacta gctctcatca
   110461 aaacaaccat tgttttgatt tctcatgaaa tttctttata cttttctccg attataaatt
   110521 cctaacccct tgagaaaatt acgcatatat ttatctccgc aaggcttaaa atttcgttgg
   110581 ccttcttttc tcaaaaatgc cccgatttcg ccttttgatg gatagatttc tgcctcagca
   110641 aaaacgtcta aaatatcatc agtggtcata gacaaggcga tttttaattt ttttagcaac
   110701 acattgttga tatatttgat ttccttgctt ttttgcggcg tgtctactat cggcttatct
   110761 ttttgcggac cacgcgccaa gagaatcagg ccatttagga atgcgtcaaa aacttggttg
   110821 ttgatggttt tttcatacac attattttct gcattatctt tgttcttatc ctgtactttg
   110881 gtcagtaccg cctgagcttc ttctttagtc agtggtacac cgcctagctc aaaagcttta
   110941 atcaaatccg tatctttcaa atcaatcgca taacgcaatc gcaataacct atcattgtta
   111001 ttcatttgtt cctccgaagt atttagtctt tactttctta cgtacttttt gtcttgctaa
   111061 gatttttagg atcaatgata aaaaccgcat cacttatgat aaggcgctgg gtatcatgag
   111121 tgaatgatat tgctgaatac aaatagtccg accacattaa gttgatcgga cttcataagc
   111181 attttattga ctaatacttt tttgcttaag aattgattct aacatctttt gctaaagtta
   111241 agcatcctat agttcctgca ttatctgcta acttaggtgt gacaatgtat tcttcgatat
   111301 ttggcgtatc gacatagccg ttaattagtt ctaaaaaacg tctttgtact ttctttttca
   111361 gatgttcttg ttgcatgaca ccaccaccaa aaattataat atctggtgaa agcatcaggg
   111421 tcgtattaca ggcacattgt gcaatataat ccgcctcgat ttcccagaac ggatcttcta
   111481 tagaaagatc ttgtcccttg gttcccgtac gcatctcgat agcgggtcca gatgccaacc
   111541 cttcgagaca atcatgatga aaaggacaag taccagagaa agtatctcct gaatgtctct
   111601 taactaacgt atgccccatt tctggatggc taaacccttc aacaaaaatc ccatcttgta
   111661 tagctcctcc accaattcca gttccaacag tgtaataaac aacactggat aaaccttttc
   111721 catgacctga aacatactcc ccgtaacaag cagcattcac atcggttgtc caggcaattg
   111781 gaatttcgaa atgcttattc aacgttccaa caaaatcaaa atttttccag gcagtttttg
   111841 gagtagaggt gatatgtcca taggtctttg attcacgatg gatatcaatc ggtccaaatg
   111901 accctactcc aatgctttct aattgttctt tatagtgatt aaagaaatca attaccaaac
   111961 tcattgtttc attcggtgta gttgtaggaa aactcactct ttctaaaacc tttaaatcat
   112021 cattgccaat ggcacaaaca aatttagtcc cacctgcttc tattgagcca tacatttctt
   112081 actccaccgt ccttttatct aggaattcgt tcttccctgt ctaatagttt ttcgaaaaac
   112141 tcatgtggtt ccttttggta ccaataggca acagaagaaa tatcgtcttg acgctcataa
   112201 agcttgatgt catcatttcc tagggtttgg aaagtaacac gtaaattttc tttgaaacta
   112261 atggggtcgg gtaaatgcca gcggtacaac ccatgcatcg gtaaagcatc atcgccaaaa
   112321 ccatgaactg gatttggatt aggttttcca gcactaaaat aatcccgggt gtgatctctt
   112381 ttagattgaa aaggatagcc cataaatagc gtattaaaat tttgagcata tgccctttct
   112441 ccaaagttct ttttatggaa agcccaagcg ccgccaaaat aatcttctgc tcctgtagat
   112501 gttattgtag ggaaatcttc atctccatct atataaaact taaattctcc ttccccccac
   112561 cagtatcttt ccaatgcgca caaagcaaag taggttccta cataataccc agtgcctttg
   112621 attccatcaa taagggtgta atcttttcct tttgtcgtga tgggttcacg gttccattta
   112681 gcatgaaaat acatcgcatc atccggtact tcagtcaatg cataattaaa tgtataaaaa
   112741 atgtgcttaa taaactcagg atgttcattc gttaaagtaa tcttagcttt ttttcgaaat
   112801 ggcatctcaa aatagcaatt catccctgat gttggattca ctacgattgg taaggaattc
   112861 acttcgcatc ttgtcccaaa cccattacaa aaaaaatctc ctaatggact ttctacagaa
   112921 ggagtggatt catcatccca ataaatacgc aaaattaaat cacgtaagac gaaactccca
   112981 gcgattgttt tgtctgtcac tgtcatccaa aaatgacgta aaattccagg gccttccatt
   113041 tctgctaaag taattgtctc tccagcattt atcggaacag aaggtgaccc ttttctccca
   113101 attcctagat gactcgctgt cattgccgct ttcccttttt caccattttt attttctggt
   113161 gaaatagttc tactttcctc tcccttgtgg agccatatat tatttaaaca attcatttta
   113221 ttcgtcctct atcctttctt cacttttttg attattcata tagatgattt gttgttgtgc
   113281 ctccgcaata attgctccaa tatctaaatt atcatttcct aactgcaaag cgattgacat
   113341 tgccaaggga agattcattc ctgcaattac gtgcactctg tcagaaatat aggtaaaaaa
   113401 cttttgattt acacttcctc cagccatgtc agttaaaaca attatttcgt ctgtagtatc
   113461 cactaactct ttaatttgtt catctatatc atcaatttct tcgacatatg cacataagct
   113521 actaatttga ttatgcatac ctgttaaaaa ctctagtgtc tctttaaaac cctcagctag
   113581 tattgaatgg ctagcaatga tcaatcttct cataattcaa atccttttcc tttacgctaa
   113641 tattccaaat gcagccataa ccatcgcaaa aacaataacg agtagaatca aaatggtcat
   113701 actgattttt ttcttactga gtagataata tgctgcagca gtaataatta ctggaattaa
   113761 tgttggtaaa attttgtcca acattggttg gattttcata ctaacttcac caaaagtgaa
   113821 tttaagtggc gttgaaacac tgataactga agcaattaat gatccaacta ctgttagccc
   113881 caatactgaa gcagcttcag ttaaataatt aatcttttca ccgtattcag taacaatctt
   113941 cattccctgc ttataaccaa attcaaagcc tctcattcgg aaaaagaaga ttaccatgat
   114001 taaaatgaac cataaaaaga ttcctacagg attattttct aaagccatat aagccgcaat
   114061 tgatcccatg atggttggga tcatcgtcat caaaagagag tccccaatac cagcaattgg
   114121 acccatcgta ccaactttaa agtcattaat cgcatctttt gcttctaatc cttgcttttc
   114181 ctccattgca acacaagcac ctaaaataat ggctgccagc caaggctgtg tattgtaata
   114241 ctgaaagtga ttatttaaag cttcgatgta ttcatcatca tctttgtata attttcttaa
   114301 aataggtgac aatgcgtaag tcacagatgc accttgctgt gtttgataat taaaggtaca
   114361 aactgacatc aaccaacgat agttcgcttt cctaagatcc tttttcgtta cttgatattt
   114421 ttcgctcatt ttattcatcc tccccaataa acccaacatc agaagctccc gcagaagcag
   114481 tgatttgtgc atctttttca gctctatgat acacattaaa tgctaaagcg aatcctatga
   114541 tcgccgctcc aataataggt accttcaaaa aagcggctaa gaaaaatcca acaagtaaat
   114601 aactaatgta tcgacctgta ggcataaagc gtaacaacat cgcaatacca acagcaggca
   114661 acatttttcc ggctaatgtt agcccatcca taaaccaatt aggtaaaaca tccaaaacgt
   114721 aattaacagc agactgtcct aaagtaacgg caataaatac tggaattgct gcagataaac
   114781 caaagaaaat tggccccaac cacaaaacac tattcatttt cttaaattct cgtttattcg
   114841 catattcctg tgatttgcga acaacaaaac catttaagat tttgacaatt acatctagtt
   114901 gcacccctaa catacctaca ggtagtccta ttgctaatcc ggcttctact ccaagctggc
   114961 taccaaatgc cgttgcaata attgcgcacg ttccatagtc cggtacagat gagccaccaa
   115021 aaccggcaac tcctaatgac atcaattgaa ctgtaccgcc aactatcaaa cctgttttta
   115081 tatcacccat caaaattcct gtgaataatc cccaaaaaac tgcgtaattt acaaagatca
   115141 tcggaatccc ataataatca acatgtttaa taaatgctaa tatagtcagc ataataattt
   115201 gaaaaatagt aaaatgcatt atccttactc ctttatccta attttgattg aatttcctgt
   115261 gccttatccg ccggaacaaa ttgtgtaaca atttctacac cagaatccaa caatttttta
   115321 tacgtgctga tattttcatc tgatgcatag gctctagaag atagctgtaa tgcattatct
   115381 ttaacaacag taccgccaac aattagtcgg tcaattctaa taccgttatc tactaaatga
   115441 aggattgttt ccggctcttt cacgatgaca aatacttttt cctgatcgta tttccccttt
   115501 ttaaaattcg caatcgctgt ttcttcagtg ataattgata cagccattcc agcaggtcta
   115561 gctaaacgca tacttgattt ttggatgtcg ttatttgcga cagaatcatc gatcaccatc
   115621 actctgtttg ctttagacat tggtgcccat tgcgtgacaa taatcccatg caataaacgg
   115681 ttatcaactc tcgacataac aatactcata aatacttcct cctttatctt tcattttctt
   115741 acaagcaatt accatgccaa aacaaaaaga acttgataaa tcaagctctt tttacataat
   115801 cataaacgta tttaacctca tgaatcggga tgtcgacact ataataatgt tcaacgacac
   115861 tgaaagattg cttaaaatgt gtctgaaatt tttgaaattc ctcactcgtt tcattgaaat
   115921 aaatttcatc ttccaaagta ttttgtttta ctaatcgttc aattaagcaa ctaatgtgaa
   115981 catataatcc gaaacacgta ttattatcaa atgaaattcc tagatcattt tgtaagatgt
   116041 atatagcatc tgaaacccgt tctaataatt tagtcggatt taatatggtc aattgctcca
   116101 ttaaattcgt tagactaaaa ttcttcagaa tattattgtt caaaactttc agttcattat
   116161 cattaaagtg tctattcaat tgattggaga atttacttat attcattcct aaaatcatat
   116221 cttcaattga aaagaattct acatcttcta gatgtggatt tagtgttcca acaataaaaa
   116281 gaatattgtt ctctttacga agctccgata aatagttttc attcactaaa gagtaatagt
   116341 cacacgtttc tatgacaaga tcagtatccc ttggaaagct atcttcaagt atctttttta
   116401 atttaaacga agttcctaat ccgcttgcac aagaacagac aatcatatcc ttcttaatat
   116461 ttgatgattc ataaatataa tccacatttt gattttcttt tacttgttca agaatctgtt
   116521 taagcggtaa atctaataat atctgatttg caacatcaat agccattctt gttgaaacat
   116581 tgtttagtag tccgaatttt ttttgagcgt ctagttccaa atttagataa attttctcca
   116641 gtgaacccat atcaacaagt aaaataattt cttcatatcc ggtacactct tgtaagtatc
   116701 tattaatttt gtctgcaatc tcactacttg aagtcattag tggcatatca atggcgtcgt
   116761 atatatatct ctcaacgatc ctattagcag tacttgctat acaggttgcc gttagatctc
   116821 catgagccaa aatcagtgct aaacgttttt gctgaaattc tttactacac attaacttaa
   116881 ataaaataac caaaacattt ttttgaattt ttgttaaata aatatcccct atttgaataa
   116941 aacgattgac taattgaact tctcgactac accaactatt aatatcattg atgaaggaat
   117001 ataatttatc gtccgatgtt aattggattt gataaatcca agaattcaaa aagatagacg
   117061 ttatttgaag ataataatag tcaaattcta tttgaaaatc tctctctatt tccttaacat
   117121 actgtctaaa tcgaatctct tgataggtgg gatatgttaa agctgaatta ttttggatat
   117181 caacattctt ttgtctaaaa tatctatcat atttctcaaa aagctgctgc ttatcagtgt
   117241 ctttcttcca cgtttgataa agatactcta taagttcgtt gatattaaaa acttcgtcta
   117301 ctttttccaa atcatccatt tgaatatatt ttaattcatt ctgtcttttc ataaccttca
   117361 tgccgttgaa attcacccat tgtggtaaat gatacatctg aatataaagt tcttccaaat
   117421 catctaagaa ataaaaggca ttggcacagg tcaattgaat gatatttttc aaattgccaa
   117481 cattttcact aaagcgatag tttaataaaa aatctaataa ttgttgagat atcagtattt
   117541 tctttgctag tctttcttct tctgatttaa aaaatatctt gattaactgt tttctttcat
   117601 aaagagaacg actgtctaat gttggaagtg ttagctttac aggtattctt ctcaacaaag
   117661 ttgataatag tttatcctca ggtggttctg atgttgcaaa tataatccag caacttgatg
   117721 tgtaccagtt ttcattatca cccatccgat gaaatttcct cgtatccata aactgaaaaa
   117781 gtttctcctg acattcattg ctcaatgcat gaatttcatc taaaaataag actccattat
   117841 tagctaattc tgctaaaccc tttcttccat cttcggctcc tgtatatgca ccttttacat
   117901 gcccaaaaaa attggacaag aaaagttctg gattattggc atattcacta caattaactg
   117961 aaatgaaaga tgccttctct tctatataat tattggccaa caaatcctta tacacataat
   118021 ttgctaaata gctttttcca gttccagtct cgccatgaag taaaatgggt aagccatttg
   118081 gcggatagta gattgctgct ttaatttgag caattacatt tcgcagactt ccttgacttc
   118141 cgataaccct ttctattcca accctaaaag agctatcata ccatttttcc gcttcctctg
   118201 aagagctaaa aatttgttga tttggaactt tctccggtaa agaaaagaag attactggac
   118261 gagtattgat ttttccaaat tctccatctt ttacgaattc attcaaatat tgagagacaa
   118321 tattacgact aacatttaat ttttcagata ttttctgtgc tgtattgttc ccccatttat
   118381 ttttaatctc agagttctct atgacataat tgacaatgcg ttcttttaat tcttccatca
   118441 cagcacctcc tcgacactat tcgaattttg ccgacagtat ttttccataa atatgacttg
   118501 ctaaaaaggc tttgtttaga tcattcaatt ggaacgttgc ttgttttgct tgatccgcta
   118561 agacgttgaa aagatactct ggcgaaatcg cataccccaa aatatcaacg attgttgcaa
   118621 tcagatcttc ttttgattct tcatctgaaa gtaattcacg atacaacatc gtttgtttgc
   118681 ttactgtgtc atattcctct aaggattcat ctgctaaaag aaccacttgt tccaacaaac
   118741 ggtcggataa atatttatag aaaatcagcc ccaataaata atttttatat tctgacgcat
   118801 ccattttact acggagatta tctgccgcac taaataactt ctgatttaat tctgcaccca
   118861 aaaagataca ctcctatctc ttatttaaag ttgctatata ccctcttatt ttagcatact
   118921 cagttttaga aaaagcaaaa aaataaagaa acttattctt gataacaagc tacgtgacgt
   118981 atatccctaa cacaaaaata gccatatcct ttggtttgga tacggctatt tgatattact
   119041 cattcaatta tcaaatcaca gtaggtgtga atctcatcgg gatcggcaat atctacttct
   119101 gattaggatc aaaggtgatt ttctcaataa aggtgtgata caaagcttta acttgcttct
   119161 tctcactctg gataagaaag caatccaaac tagtcaatat ctgttggaca tctttgactt
   119221 gaatcggttc cccttgttgt tgaaagatcc ctaagatttc attctctcga atttgtaact
   119281 gtcttcgttt ttcagtcaat tcatttaaac gatcttttaa catcggaaac aattcgggac
   119341 tatcttccaa agcagcttcc tgtttttgct ctaatagtta gattttgcgt accattttct
   119401 tcttttattt tccttcgatt gctgatttta atatagtcta aaggaaattt atatttattg
   119461 tattctttat aacttcaaga gaacttcgtt tgagtattac tgtatttttt taattccata
   119521 agaaaattag ttattacctg ccacctaatc agcaaatctg aacctgagtc aatataatta
   119581 atattatttt ccatctccaa cattgtaagt actgttggtg ttaatatagg gactttgtac
   119641 aattgtattt ttttgatttt atcatctgca caaaggagat tttttaaatc taatccttct
   119701 tttatttcaa aagttgaatt ttttaaatct ataagagtaa aaccaattcc ttcatactga
   119761 atttctgaaa atcgaatttt tctcccagtt gatagatcaa ggggcagaaa accttttatt
   119821 acattgtgag tgtcaccatc atttacaatg tcattaggaa ttttgatttt agaattctgt
   119881 ggagcaccta ttggaagata ataattcatt ataattttgt agtcttcatg aggaaataaa
   119941 gtacttttag gattgctaag atattctcgt accgaatcta agaatggctt atccattgaa
   120001 tcttcatccc catttaaact aaaaaacatt cctacaattg cttttgcaac tctaagtgaa
   120061 ttcattttct ttattgaatg tgttaaatca gacttaccat tctttaaact tgtattgtta
   120121 taaaggaaat ttttattgtc ttctgcaaat ctaatatatt cctcaacata ctcactacca
   120181 aaaaaactat tacatttact acataacgta ttggaatggc aacagttttt ttgacaaatt
   120241 ttataaggtg cagaacttct ttccgtatgc tattccgatt gtccttgaca atgagcctcc
   120301 tcggatgtga cgatcttcgg caggagctaa cagttaaagt tagactgcct cgctagcgtt
   120361 agcacatccg agctcattat caaggattcg ctgcgccaat
//
LOCUS       DANJLF010000002        95905 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000002 DANJLF010000000
VERSION     DANJLF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 95905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..562
                     /locus_tag="PWT25_000113"
     CDS             <1..562
                     /locus_tag="PWT25_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL1020229.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="PWT25_000114"
     CDS             762..1076
                     /locus_tag="PWT25_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL1020230.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="PWT25_000115"
     CDS             1097..1474
                     /locus_tag="PWT25_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL1020231.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="PWT25_000116"
     CDS             1484..2257
                     /locus_tag="PWT25_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020232.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="PWT25_000117"
     CDS             2279..3682
                     /locus_tag="PWT25_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL1020233.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="PWT25_000118"
     CDS             3864..5048
                     /locus_tag="PWT25_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL1020234.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="PWT25_000119"
     CDS             5045..5335
                     /locus_tag="PWT25_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020235.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="PWT25_000120"
     CDS             5332..5553
                     /locus_tag="PWT25_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020236.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="PWT25_000121"
     CDS             5595..6374
                     /locus_tag="PWT25_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDL1020237.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="PWT25_000122"
     CDS             6441..6935
                     /locus_tag="PWT25_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL1020238.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="PWT25_000123"
     CDS             6991..7629
                     /locus_tag="PWT25_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020239.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="PWT25_000124"
     CDS             7701..8096
                     /locus_tag="PWT25_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1020240.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="PWT25_000125"
     CDS             8080..10533
                     /locus_tag="PWT25_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1020241.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="PWT25_000126"
     CDS             10530..12557
                     /locus_tag="PWT25_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020242.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="PWT25_000127"
     CDS             12554..13576
                     /locus_tag="PWT25_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL1020243.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="PWT25_000128"
     CDS             13593..14522
                     /locus_tag="PWT25_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1020244.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="PWT25_000129"
     CDS             14767..14883
                     /locus_tag="PWT25_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL1020245.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="PWT25_000130"
     CDS             14899..15702
                     /locus_tag="PWT25_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL1020246.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15937..16173
                     /locus_tag="PWT25_000131"
     CDS             15937..16173
                     /locus_tag="PWT25_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020247.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="PWT25_000132"
     CDS             16180..18132
                     /locus_tag="PWT25_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL1020248.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="PWT25_000133"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="PWT25_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL1020249.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19063..20103
                     /locus_tag="PWT25_000134"
     CDS             19063..20103
                     /locus_tag="PWT25_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL1020250.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20201..20389
                     /locus_tag="PWT25_000135"
     CDS             20201..20389
                     /locus_tag="PWT25_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL1020251.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20447..20800)
                     /locus_tag="PWT25_000136"
     CDS             complement(20447..20800)
                     /locus_tag="PWT25_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020252.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21331..21753
                     /locus_tag="PWT25_000137"
     CDS             21331..21753
                     /locus_tag="PWT25_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1020253.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21750..21980
                     /locus_tag="PWT25_000138"
     CDS             21750..21980
                     /locus_tag="PWT25_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020254.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22478..22678
                     /locus_tag="PWT25_000139"
     CDS             22478..22678
                     /locus_tag="PWT25_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL1020255.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22705..23898
                     /locus_tag="PWT25_000140"
     CDS             22705..23898
                     /locus_tag="PWT25_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1020256.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="PWT25_000141"
     CDS             complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="PWT25_000141"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL1020257.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25590..26513)
                     /locus_tag="PWT25_000142"
     CDS             complement(25590..26513)
                     /locus_tag="PWT25_000142"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL1020258.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26709..27314
                     /locus_tag="PWT25_000143"
     CDS             26709..27314
                     /locus_tag="PWT25_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1020259.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27622..28638
                     /locus_tag="PWT25_000144"
     CDS             27622..28638
                     /locus_tag="PWT25_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1020260.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28779..30080
                     /locus_tag="PWT25_000145"
     CDS             28779..30080
                     /locus_tag="PWT25_000145"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL1020261.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30126..30788
                     /gene="deoC"
                     /locus_tag="PWT25_000146"
     CDS             30126..30788
                     /gene="deoC"
                     /locus_tag="PWT25_000146"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL1020262.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30809..31207
                     /locus_tag="PWT25_000147"
     CDS             30809..31207
                     /locus_tag="PWT25_000147"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL1020263.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31273..32355
                     /locus_tag="PWT25_000148"
     CDS             31273..32355
                     /locus_tag="PWT25_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1020264.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32567..34129
                     /locus_tag="PWT25_000149"
     CDS             32567..34129
                     /locus_tag="PWT25_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020265.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34122..35255
                     /locus_tag="PWT25_000150"
     CDS             34122..35255
                     /locus_tag="PWT25_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020266.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35255..36211
                     /locus_tag="PWT25_000151"
     CDS             35255..36211
                     /locus_tag="PWT25_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020267.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36310..37476
                     /gene="deoB"
                     /locus_tag="PWT25_000152"
     CDS             36310..37476
                     /gene="deoB"
                     /locus_tag="PWT25_000152"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL1020268.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37500..38318
                     /locus_tag="PWT25_000153"
     CDS             37500..38318
                     /locus_tag="PWT25_000153"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL1020269.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38335..39042
                     /gene="deoD"
                     /locus_tag="PWT25_000154"
     CDS             38335..39042
                     /gene="deoD"
                     /locus_tag="PWT25_000154"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL1020270.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="PWT25_000155"
     CDS             complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="PWT25_000155"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL1020271.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40198..40779)
                     /locus_tag="PWT25_000156"
     CDS             complement(40198..40779)
                     /locus_tag="PWT25_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1020272.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="PWT25_000157"
     CDS             complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="PWT25_000157"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL1020273.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41914..42327
                     /gene="rpsL"
                     /locus_tag="PWT25_000158"
     CDS             41914..42327
                     /gene="rpsL"
                     /locus_tag="PWT25_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL1020274.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42411..42881
                     /gene="rpsG"
                     /locus_tag="PWT25_000159"
     CDS             42411..42881
                     /gene="rpsG"
                     /locus_tag="PWT25_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL1020275.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42975..45062
                     /gene="fusA"
                     /locus_tag="PWT25_000160"
     CDS             42975..45062
                     /gene="fusA"
                     /locus_tag="PWT25_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL1020276.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45234..46421
                     /gene="tuf"
                     /locus_tag="PWT25_000161"
     CDS             45234..46421
                     /gene="tuf"
                     /locus_tag="PWT25_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL1020277.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46828..47136
                     /gene="rpsJ"
                     /locus_tag="PWT25_000162"
     CDS             46828..47136
                     /gene="rpsJ"
                     /locus_tag="PWT25_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL1020278.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47167..47796
                     /gene="rplC"
                     /locus_tag="PWT25_000163"
     CDS             47167..47796
                     /gene="rplC"
                     /locus_tag="PWT25_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL1020279.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47824..48447
                     /gene="rplD"
                     /locus_tag="PWT25_000164"
     CDS             47824..48447
                     /gene="rplD"
                     /locus_tag="PWT25_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL1020280.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48447..48737
                     /gene="rplW"
                     /locus_tag="PWT25_000165"
     CDS             48447..48737
                     /gene="rplW"
                     /locus_tag="PWT25_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL1020281.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48776..49609
                     /gene="rplB"
                     /locus_tag="PWT25_000166"
     CDS             48776..49609
                     /gene="rplB"
                     /locus_tag="PWT25_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL1020282.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49652..49930
                     /gene="rpsS"
                     /locus_tag="PWT25_000167"
     CDS             49652..49930
                     /gene="rpsS"
                     /locus_tag="PWT25_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL1020283.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49953..50300
                     /gene="rplV"
                     /locus_tag="PWT25_000168"
     CDS             49953..50300
                     /gene="rplV"
                     /locus_tag="PWT25_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL1020284.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50314..50970
                     /gene="rpsC"
                     /locus_tag="PWT25_000169"
     CDS             50314..50970
                     /gene="rpsC"
                     /locus_tag="PWT25_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL1020285.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50973..51407
                     /gene="rplP"
                     /locus_tag="PWT25_000170"
     CDS             50973..51407
                     /gene="rplP"
                     /locus_tag="PWT25_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL1020286.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51397..51585
                     /gene="rpmC"
                     /locus_tag="PWT25_000171"
     CDS             51397..51585
                     /gene="rpmC"
                     /locus_tag="PWT25_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL1020287.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51610..51876
                     /gene="rpsQ"
                     /locus_tag="PWT25_000172"
     CDS             51610..51876
                     /gene="rpsQ"
                     /locus_tag="PWT25_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL1020288.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51934..52302
                     /gene="rplN"
                     /locus_tag="PWT25_000173"
     CDS             51934..52302
                     /gene="rplN"
                     /locus_tag="PWT25_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL1020289.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52339..52647
                     /gene="rplX"
                     /locus_tag="PWT25_000174"
     CDS             52339..52647
                     /gene="rplX"
                     /locus_tag="PWT25_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL1020290.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52675..53214
                     /gene="rplE"
                     /locus_tag="PWT25_000175"
     CDS             52675..53214
                     /gene="rplE"
                     /locus_tag="PWT25_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL1020291.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53233..53418
                     /locus_tag="PWT25_000176"
     CDS             53233..53418
                     /locus_tag="PWT25_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL1020292.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53454..53852
                     /gene="rpsH"
                     /locus_tag="PWT25_000177"
     CDS             53454..53852
                     /gene="rpsH"
                     /locus_tag="PWT25_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL1020293.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53884..54420
                     /gene="rplF"
                     /locus_tag="PWT25_000178"
     CDS             53884..54420
                     /gene="rplF"
                     /locus_tag="PWT25_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL1020294.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54581..54937
                     /gene="rplR"
                     /locus_tag="PWT25_000179"
     CDS             54581..54937
                     /gene="rplR"
                     /locus_tag="PWT25_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL1020295.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54958..55458
                     /gene="rpsE"
                     /locus_tag="PWT25_000180"
     CDS             54958..55458
                     /gene="rpsE"
                     /locus_tag="PWT25_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL1020296.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55473..55652
                     /gene="rpmD"
                     /locus_tag="PWT25_000181"
     CDS             55473..55652
                     /gene="rpmD"
                     /locus_tag="PWT25_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL1020297.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55696..56136
                     /gene="rplO"
                     /locus_tag="PWT25_000182"
     CDS             55696..56136
                     /gene="rplO"
                     /locus_tag="PWT25_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL1020298.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56136..57431
                     /gene="secY"
                     /locus_tag="PWT25_000183"
     CDS             56136..57431
                     /gene="secY"
                     /locus_tag="PWT25_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL1020299.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57493..58140
                     /locus_tag="PWT25_000184"
     CDS             57493..58140
                     /locus_tag="PWT25_000184"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL1020300.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58331..58549
                     /gene="infA"
                     /locus_tag="PWT25_000185"
     CDS             58331..58549
                     /gene="infA"
                     /locus_tag="PWT25_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL1020301.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58582..58698
                     /gene="rpmJ"
                     /locus_tag="PWT25_000186"
     CDS             58582..58698
                     /gene="rpmJ"
                     /locus_tag="PWT25_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL1020302.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58716..59081
                     /gene="rpsM"
                     /locus_tag="PWT25_000187"
     CDS             58716..59081
                     /gene="rpsM"
                     /locus_tag="PWT25_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL1020303.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59109..59498
                     /gene="rpsK"
                     /locus_tag="PWT25_000188"
     CDS             59109..59498
                     /gene="rpsK"
                     /locus_tag="PWT25_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL1020304.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59578..60516
                     /locus_tag="PWT25_000189"
     CDS             59578..60516
                     /locus_tag="PWT25_000189"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL1020305.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60544..60924
                     /gene="rplQ"
                     /locus_tag="PWT25_000190"
     CDS             60544..60924
                     /gene="rplQ"
                     /locus_tag="PWT25_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL1020306.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61196..62035
                     /locus_tag="PWT25_000191"
     CDS             61196..62035
                     /locus_tag="PWT25_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1020307.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62011..62880
                     /locus_tag="PWT25_000192"
     CDS             62011..62880
                     /locus_tag="PWT25_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1020308.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62870..63667
                     /locus_tag="PWT25_000193"
     CDS             62870..63667
                     /locus_tag="PWT25_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL1020309.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63870..64616
                     /gene="truA"
                     /locus_tag="PWT25_000194"
     CDS             63870..64616
                     /gene="truA"
                     /locus_tag="PWT25_000194"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL1020310.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64949..67618)
                     /locus_tag="PWT25_000195"
     CDS             complement(64949..67618)
                     /locus_tag="PWT25_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL1020311.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67856..68044)
                     /locus_tag="PWT25_000196"
     CDS             complement(67856..68044)
                     /locus_tag="PWT25_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL1020312.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68314..68676
                     /locus_tag="PWT25_000197"
     CDS             68314..68676
                     /locus_tag="PWT25_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1020313.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68728..70410
                     /locus_tag="PWT25_000198"
     CDS             68728..70410
                     /locus_tag="PWT25_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL1020314.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70527..72563
                     /gene="recG"
                     /locus_tag="PWT25_000199"
     CDS             70527..72563
                     /gene="recG"
                     /locus_tag="PWT25_000199"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL1020315.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72612..73613
                     /gene="plsX"
                     /locus_tag="PWT25_000200"
     CDS             72612..73613
                     /gene="plsX"
                     /locus_tag="PWT25_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL1020316.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73735..73977
                     /gene="acpP"
                     /locus_tag="PWT25_000201"
     CDS             73735..73977
                     /gene="acpP"
                     /locus_tag="PWT25_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL1020317.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            74245..75249
                     /locus_tag="PWT25_000202"
     CDS             74245..75249
                     /locus_tag="PWT25_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020318.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            75250..76194
                     /locus_tag="PWT25_000203"
     CDS             75250..76194
                     /locus_tag="PWT25_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020319.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            76194..77156
                     /locus_tag="PWT25_000204"
     CDS             76194..77156
                     /locus_tag="PWT25_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020320.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            77183..78097
                     /locus_tag="PWT25_000205"
     CDS             77183..78097
                     /locus_tag="PWT25_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020321.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            78123..79904
                     /locus_tag="PWT25_000206"
     CDS             78123..79904
                     /locus_tag="PWT25_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1020322.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            80252..80938
                     /locus_tag="PWT25_000207"
     CDS             80252..80938
                     /locus_tag="PWT25_000207"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL1020323.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            80958..84539
                     /gene="smc"
                     /locus_tag="PWT25_000208"
     CDS             80958..84539
                     /gene="smc"
                     /locus_tag="PWT25_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL1020324.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            84548..85357
                     /locus_tag="PWT25_000209"
     CDS             84548..85357
                     /locus_tag="PWT25_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1020325.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            85368..86366
                     /gene="ftsY"
                     /locus_tag="PWT25_000210"
     CDS             85368..86366
                     /gene="ftsY"
                     /locus_tag="PWT25_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL1020326.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            86553..88820
                     /gene="gshAB"
                     /locus_tag="PWT25_000211"
     CDS             86553..88820
                     /gene="gshAB"
                     /locus_tag="PWT25_000211"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL1020327.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(88909..89361)
                     /locus_tag="PWT25_000212"
     CDS             complement(88909..89361)
                     /locus_tag="PWT25_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL1020328.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            89511..90461
                     /locus_tag="PWT25_000213"
     CDS             89511..90461
                     /locus_tag="PWT25_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL1020329.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            90454..91413
                     /locus_tag="PWT25_000214"
     CDS             90454..91413
                     /locus_tag="PWT25_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL1020330.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            91410..92165
                     /locus_tag="PWT25_000215"
     CDS             91410..92165
                     /locus_tag="PWT25_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1020331.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            92188..93141
                     /locus_tag="PWT25_000216"
     CDS             92188..93141
                     /locus_tag="PWT25_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1020332.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(93785..94081)
                     /locus_tag="PWT25_000217"
     CDS             complement(93785..94081)
                     /locus_tag="PWT25_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL1020333.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            94436..94792
                     /locus_tag="PWT25_000218"
     CDS             94436..94792
                     /locus_tag="PWT25_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL1020334.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            94802..95701
                     /locus_tag="PWT25_000219"
     CDS             94802..95701
                     /locus_tag="PWT25_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL1020335.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        31584 a        17030 c        20163 g        27128 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    20161 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    20221 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20281 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20341 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20401 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20461 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20521 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20581 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20641 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20701 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20761 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20821 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
    20881 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
    20941 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
    21001 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
    21061 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
    21121 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
    21181 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
    21241 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
    21301 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
    21361 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
    21421 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
    21481 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
    21541 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
    21601 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
    21661 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
    21721 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
    21781 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
    21841 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
    21901 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
    21961 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
    22021 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
    22081 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
    22141 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
    22201 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
    22261 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
    22321 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
    22381 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
    22441 ggagttttcc tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga
    22501 tttagttgag ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga
    22561 attgatgatt acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg
    22621 tgaagctgtt gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt
    22681 tttgcacagt aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga
    22741 acctattatt ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat
    22801 cgtagtggat tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg
    22861 aaaccagaag aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa
    22921 attttccttc catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca
    22981 actattaaga agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt
    23041 gatgtgcaaa attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct
    23101 gttcaaggac tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca
    23161 aatccatcta agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca
    23221 aaagaagaat ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt
    23281 ttatttatct ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc
    23341 attcaatggg aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat
    23401 tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat
    23461 attgtattag ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat
    23521 ctaattcaga cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta
    23581 tcatatgcta ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg
    23641 ttacggcatt ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa
    23701 gatcgtctag gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct
    23761 aacagtaatt tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa
    23821 caaaattatg atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa
    23881 atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat
    23941 aagaggattt ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag
    24001 tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt
    24061 tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta
    24121 cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc
    24181 ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga
    24241 tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt
    24301 cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat
    24361 caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg
    24421 gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga
    24481 tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta
    24541 ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga
    24601 attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta
    24661 agaagcgatc tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt
    24721 ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca
    24781 attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg
    24841 aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat
    24901 cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc
    24961 gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa
    25021 aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt
    25081 caaagccttc aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt
    25141 ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat
    25201 attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac
    25261 catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg
    25321 cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg
    25381 cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac
    25441 gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt
    25501 caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta
    25561 tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg
    25621 atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat
    25681 tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct
    25741 tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc
    25801 agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca
    25861 tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc
    25921 gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga
    25981 atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt
    26041 acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg
    26101 tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc
    26161 agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt
    26221 gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg
    26281 tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga
    26341 taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca
    26401 ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa
    26461 aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc
    26521 tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact
    26581 taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt
    26641 cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga
    26701 tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac
    26761 aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt
    26821 cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt
    26881 tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt
    26941 tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc
    27001 tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata
    27061 tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg
    27121 ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg
    27181 gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg
    27241 aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa
    27301 gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc
    27361 atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt
    27421 ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag
    27481 atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa
    27541 catactttcc ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt
    27601 agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc
    27661 gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag
    27721 acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct
    27781 tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat
    27841 ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg
    27901 atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt
    27961 tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat
    28021 gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat
    28081 cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg
    28141 ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat
    28201 caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt
    28261 aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa
    28321 aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc
    28381 ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata
    28441 ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag
    28501 ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct
    28561 caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa
    28621 gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt
    28681 tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat
    28741 aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg
    28801 aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact
    28861 atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc
    28921 aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg
    28981 tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg
    29041 gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta
    29101 aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc
    29161 ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag
    29221 tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc
    29281 gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa
    29341 aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca
    29401 tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg
    29461 ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg
    29521 gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc
    29581 tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga
    29641 caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa
    29701 aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac
    29761 gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa
    29821 aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca
    29881 aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg
    29941 tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca
    30001 ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc
    30061 atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa
    30121 aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag
    30181 aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca
    30241 atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta
    30301 cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag
    30361 atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat
    30421 caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc
    30481 gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag
    30541 cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa
    30601 caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg
    30661 gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg
    30721 gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag
    30781 gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac
    30841 agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg
    30901 tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg
    30961 attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac
    31021 atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg
    31081 tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa
    31141 tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga
    31201 tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga
    31261 ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca
    31321 gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc
    31381 tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca
    31441 ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga
    31501 ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt
    31561 gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca
    31621 gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc
    31681 atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt
    31741 gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta
    31801 aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg
    31861 aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca
    31921 gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat
    31981 gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc
    32041 gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca
    32101 ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta
    32161 gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt
    32221 tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa
    32281 aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact
    32341 cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg
    32401 gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg
    32461 gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt
    32521 agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa
    32581 tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa
    32641 catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg
    32701 caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt
    32761 gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg
    32821 gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt
    32881 gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca
    32941 aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc
    33001 agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact
    33061 gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat
    33121 catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga
    33181 aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt
    33241 caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc
    33301 attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct
    33361 agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc
    33421 tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc
    33481 tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac
    33541 taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca
    33601 taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca
    33661 tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag
    33721 agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt
    33781 atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct
    33841 gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa
    33901 acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga
    33961 agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat
    34021 cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt
    34081 agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa
    34141 aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga
    34201 tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag
    34261 cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta
    34321 cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac
    34381 aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa
    34441 aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg
    34501 ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga
    34561 tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct
    34621 atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat
    34681 ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg
    34741 cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg
    34801 ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt
    34861 tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa
    34921 cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg
    34981 tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta
    35041 ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca
    35101 atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca
    35161 tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac
    35221 caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt
    35281 gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac
    35341 tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc
    35401 ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg
    35461 gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc
    35521 aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc
    35581 tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac
    35641 gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat
    35701 tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg
    35761 ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca
    35821 agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc
    35881 tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc
    35941 agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg
    36001 gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat
    36061 cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc
    36121 atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc
    36181 aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac
    36241 gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa
    36301 ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa
    36361 gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa
    36421 gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt
    36481 gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct
    36541 gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca
    36601 tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct
    36661 ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt
    36721 gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag
    36781 atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt
    36841 cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg
    36901 ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc
    36961 ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag
    37021 atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg
    37081 gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc
    37141 aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat
    37201 gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac
    37261 ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg
    37321 cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct
    37381 caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact
    37441 gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa
    37501 tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa
    37561 aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg
    37621 aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg
    37681 ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag
    37741 ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga
    37801 tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat
    37861 catttgtacc aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc
    37921 ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt
    37981 atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta
    38041 aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa
    38101 tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag
    38161 cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta
    38221 tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt
    38281 ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt
    38341 gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct
    38401 ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta
    38461 cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca
    38521 ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta
    38581 aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat
    38641 ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc
    38701 tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa
    38761 gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat
    38821 gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca
    38881 gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt
    38941 agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat
    39001 gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct
    39061 gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg
    39121 ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct
    39181 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    39241 tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact
    39301 gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc
    39361 aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc
    39421 catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc
    39481 atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca
    39541 gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt
    39601 gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa
    39661 tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc
    39721 agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca
    39781 tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt
    39841 gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat
    39901 tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca
    39961 accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt
    40021 tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt
    40081 tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa
    40141 acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta
    40201 gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac
    40261 tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg
    40321 tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt
    40381 acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc
    40441 taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc
    40501 aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg
    40561 cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc
    40621 agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc
    40681 atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac
    40741 gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg
    40801 attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt
    40861 ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg
    40921 acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat
    40981 gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct
    41041 ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt
    41101 taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat
    41161 atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag
    41221 tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac
    41281 tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat
    41341 ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg
    41401 tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg
    41461 taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc
    41521 ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa
    41581 ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac
    41641 tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt
    41701 caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg
    41761 ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa
    41821 ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac
    41881 tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt
    41941 aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac
    42001 agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt
    42061 gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt
    42121 ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa
    42181 cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat
    42241 atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa
    42301 tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta
    42361 ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta
    42421 aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa
    42481 ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct
    42541 ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac
    42601 aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa
    42661 actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg
    42721 tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa
    42781 tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa
    42841 tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg
    42901 gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag
    42961 gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca
    43021 tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta
    43081 aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag
    43141 aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc
    43201 gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat
    43261 tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa
    43321 ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca
    43381 aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc
    43441 aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta
    43501 tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag
    43561 aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg
    43621 aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca
    43681 ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag
    43741 tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg
    43801 attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg
    43861 aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta
    43921 tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat
    43981 caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac
    44041 agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg
    44101 ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta
    44161 ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa
    44221 aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt
    44281 tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc
    44341 acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg
    44401 cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg
    44461 tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg
    44521 aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat
    44581 acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct
    44641 atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa
    44701 atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta
    44761 atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag
    44821 gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg
    44881 ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta
    44941 caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg
    45001 aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat
    45061 aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat atttttaata
    45121 tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat
    45181 atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa
    45241 aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc
    45301 atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc
    45361 aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa
    45421 tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc
    45481 caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta
    45541 tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt
    45601 ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg
    45661 acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat
    45721 tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg
    45781 cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc
    45841 cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg
    45901 gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag
    45961 ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt
    46021 tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg
    46081 ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc
    46141 atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc
    46201 cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg
    46261 ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg
    46321 aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac
    46381 gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt
    46441 aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga
    46501 gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca
    46561 cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca
    46621 agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta
    46681 gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca
    46741 cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt
    46801 ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa
    46861 gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga
    46921 actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc
    46981 atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa
    47041 cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac
    47101 ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt
    47161 gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa
    47221 tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg
    47281 aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa
    47341 gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc
    47401 ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca
    47461 gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga
    47521 ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt
    47581 taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa
    47641 cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt
    47701 gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct
    47761 ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa
    47821 ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt
    47881 aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg
    47941 caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc
    48001 ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc
    48061 tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac
    48121 aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct
    48181 gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc
    48241 aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt
    48301 aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat
    48361 aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt
    48421 actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga
    48481 tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc
    48541 gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa
    48601 acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata
    48661 caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat
    48721 tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc
    48781 gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc
    48841 tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg
    48901 acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata
    48961 tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga
    49021 atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc
    49081 ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc
    49141 agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca
    49201 caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc
    49261 tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg
    49321 tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt
    49381 aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg
    49441 tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg
    49501 acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa
    49561 aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc
    49621 atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt
    49681 tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt
    49741 tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc
    49801 agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa
    49861 attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac
    49921 gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag
    49981 caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca
    50041 gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg
    50101 gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag
    50161 atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt
    50221 tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg
    50281 tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga
    50341 atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct
    50401 gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct
    50461 gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca
    50521 gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa
    50581 ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat
    50641 ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc
    50701 cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa
    50761 actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa
    50821 ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat
    50881 acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact
    50941 aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc
    51001 gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg
    51061 gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg
    51121 ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc
    51181 ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac
    51241 ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc
    51301 ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta
    51361 agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa
    51421 ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt
    51481 agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa
    51541 tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc
    51601 attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca
    51661 gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat
    51721 ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa
    51781 gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt
    51841 ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga
    51901 ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa
    51961 gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt
    52021 cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt
    52081 ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct
    52141 cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat
    52201 gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac
    52261 aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa
    52321 aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga
    52381 aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt
    52441 gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt
    52501 ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact
    52561 ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa
    52621 aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac
    52681 cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat
    52741 agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat
    52801 gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt
    52861 caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga
    52921 atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa
    52981 ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt
    53041 gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat
    53101 tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct
    53161 gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag
    53221 gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt
    53281 tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag
    53341 ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg
    53401 aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca
    53461 tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg
    53521 agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg
    53581 aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt
    53641 tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac
    53701 caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta
    53761 tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata
    53821 tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt
    53881 taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa
    53941 gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca
    54001 gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa
    54061 gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc
    54121 agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa
    54181 ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct
    54241 ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa
    54301 gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt
    54361 aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa
    54421 tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac
    54481 cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg
    54541 cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa
    54601 gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga
    54661 gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga
    54721 cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac
    54781 aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg
    54841 cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt
    54901 agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg
    54961 gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta
    55021 acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac
    55081 aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt
    55141 aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc
    55201 ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa
    55261 ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca
    55321 gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt
    55381 gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt
    55441 gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac
    55501 gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa
    55561 agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg
    55621 tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa
    55681 ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta
    55741 cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa
    55801 aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca
    55861 ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca
    55921 gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct
    55981 ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg
    56041 gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca
    56101 attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt
    56161 ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc
    56221 gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt
    56281 ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca
    56341 ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa
    56401 tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac
    56461 tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca
    56521 ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt
    56581 acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac
    56641 agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt
    56701 ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt
    56761 cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg
    56821 tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac
    56881 gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta
    56941 ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga
    57001 gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata
    57061 cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct
    57121 ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac
    57181 caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa
    57241 gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg
    57301 tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg
    57361 tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat
    57421 tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta
    57481 ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa
    57541 gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc
    57601 gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt
    57661 gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat
    57721 acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg
    57781 gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt
    57841 gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact
    57901 taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat
    57961 gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac
    58021 atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt
    58081 gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag
    58141 cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat
    58201 gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt
    58261 gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga
    58321 ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt
    58381 gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc
    58441 aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt
    58501 gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt
    58561 aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat
    58621 tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat
    58681 aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata
    58741 ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta
    58801 ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa
    58861 cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc
    58921 ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa
    58981 tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc
    59041 gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt
    59101 aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag
    59161 caggtattgc acatatccat tctacattca ataacactat catcatgatc actgatactc
    59221 acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat
    59281 caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg
    59341 gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc
    59401 gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc
    59461 ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt
    59521 ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg
    59581 attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc
    59641 atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt
    59701 ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat
    59761 gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa
    59821 ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga
    59881 cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa
    59941 gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa
    60001 cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt
    60061 cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca
    60121 cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca
    60181 attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt
    60241 tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca
    60301 caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac
    60361 aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct
    60421 gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg
    60481 cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc
    60541 aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat
    60601 ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa
    60661 gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt
    60721 cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac
    60781 atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa
    60841 cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca
    60901 atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt
    60961 ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag
    61021 cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc
    61081 tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa
    61141 ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga
    61201 gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc
    61261 attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa
    61321 tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg
    61381 gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat
    61441 ggttggaatg atttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga
    61501 cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa
    61561 agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc
    61621 aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat
    61681 tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac
    61741 gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga
    61801 tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg
    61861 tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc
    61921 atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata
    61981 tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc
    62041 acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc
    62101 aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt
    62161 cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat
    62221 attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc
    62281 tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt
    62341 cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa
    62401 caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa
    62461 atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat
    62521 gaaccaacgg caggacttga tccccagggc agaaaagaaa tgatggagat gttttggcgt
    62581 cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc
    62641 aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg
    62701 caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc
    62761 accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca
    62821 gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa
    62881 attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc
    62941 taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac
    63001 gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa
    63061 attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca
    63121 ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga
    63181 atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc
    63241 gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct
    63301 tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc
    63361 tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc
    63421 tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt
    63481 gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga
    63541 agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa
    63601 tagagacacg atggttatca ttgcttttgt attattaact gtgctactga tctttttaag
    63661 aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag
    63721 gatgcaagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct
    63781 ctttctttta tctgaaaaga aagaacgaaa aaattttata gaaaaatcat tattccagat
    63841 aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcgtacg
    63901 acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag
    63961 tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa
    64021 ggacagatgc cggagtacat gcgatcgggc aagtaatcca ttttgattat ccagaggagc
    64081 gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa
    64141 gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt
    64201 atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt
    64261 attttcctta cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga
    64321 ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa atacgtacga
    64381 tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg
    64441 gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca
    64501 atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag
    64561 caggacctac agcacaccct gaaggacttt atttgtatga agtgaaatat gaataagagg
    64621 ttatgataga agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa
    64681 aattttgcaa aatttcggct tattttcgaa gaagctactt ctggaacacc gtttaagaaa
    64741 gaggtcgtga gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc
    64801 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    64861 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg
    64921 gacagtctga acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc
    64981 gtggttgttc ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt
    65041 accagacata aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa
    65101 atccttcagg caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca
    65161 agaatgcgtc tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc
    65221 ctttgtcata aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag
    65281 aagcttttct gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg
    65341 cttgataggc tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg
    65401 tcccataaaa acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat
    65461 agtattgacc agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg
    65521 cactagaact ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat
    65581 ttgcaggcac atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt
    65641 aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg
    65701 tatagaatgt aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac
    65761 tggtcaaatt atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg
    65821 ctagtggcaa tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc
    65881 ggaagccata tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa
    65941 acccatccat cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac
    66001 ggaaacctaa tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac
    66061 tgataccact gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca
    66121 agcggtaaaa cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat
    66181 aaggatcagt atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca
    66241 tactgttcgt attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat
    66301 gattgaatga aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga
    66361 aaaccactgt caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga
    66421 tgccccatgc caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac
    66481 catcgtcttg ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga
    66541 acagataggc ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat
    66601 tcaatggaac aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt
    66661 tatttttcaa cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca
    66721 gacctgcgta aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat
    66781 ttttcatgat tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca
    66841 gtgcctcgcc atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc
    66901 cgcctgccag taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt
    66961 ttgtcaatag acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat
    67021 aaaaactgga taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat
    67081 ccataacacg atgaatcccc aaaatcacta atggaagata aacaaatgca tccagccaca
    67141 tgatgatctc cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa
    67201 catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac
    67261 agccgacctt aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa
    67321 agaacaccag tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca
    67381 atcctagaga agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga
    67441 agctggcatg aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc
    67501 ttccaggata gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga
    67561 aactagctgc tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat
    67621 ttattttcca ccaaatttct atgaagttgt attcagttta tcatatcctt gtttttctct
    67681 gcttaaaaag agtctagttt tcacaaaaaa ataaaattta gaataaataa cttcataatt
    67741 tgttcgtata acaaaaataa acgcaaaaaa agggtaggaa tcaaaagtcc cacccttaaa
    67801 aatttttatt aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa
    67861 cgcgttcaac ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc
    67921 cgtcgaccat aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca
    67981 tagcgtgtga acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg
    68041 ccatggtaat ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag
    68101 actaacatat catatagaat gggaatttgc aagtacattt tcatagatac aattgacaat
    68161 tgagaagaat aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt
    68221 actttgggta actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa
    68281 tgggagtttt ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct
    68341 ggaaccattg aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat
    68401 gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt
    68461 ttgcgtaaag aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct
    68521 gtagatgttt atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc
    68581 caagaaaaag tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat
    68641 gtctttgtac aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag
    68701 cagtcgatct gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc
    68761 caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca
    68821 ttaaatgtat tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact
    68881 agtggagcta aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg
    68941 ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg
    69001 tttcgtggat tttctaagca aattccagat gttgtctcat taaatgcttc tgatcttgct
    69061 gcagccttca ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga
    69121 acgattttga cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact
    69181 gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag caaagcgtgc tttagctaaa
    69241 actccagact tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga
    69301 ctgctgtttg tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa
    69361 acatatgaac cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt
    69421 gtccaaggtc aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta
    69481 aaaatcggag aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat
    69541 ttagatggta taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac
    69601 gtgcatacag aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc
    69661 aaaatcaaag tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa
    69721 gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt atggtatcgt agcgatcgct
    69781 gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt
    69841 ggacaaacaa tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca
    69901 gataaagtga ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca
    69961 gaagtagcag atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca
    70021 gctatgcttt cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa
    70081 atgttgtcta gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac
    70141 ggtgtagaga tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct
    70201 aacccagata tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca
    70261 gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct
    70321 gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct
    70381 gtctatcctt atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag
    70441 tattcattca taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt
    70501 tttgttaaag gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg
    70561 caggagtcgg agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag
    70621 acttactgac ttattatcct tttcgctacg aagatattca ggaaagaaat ctaaacgaga
    70681 tccaggatca agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc
    70741 gatttggtta taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca
    70801 acgtttcttt ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg
    70861 ccgtatacgg taaatgggat gcaaagcgta aagccttgaa cgggatgaag attttaggtt
    70921 ctcaaacaat ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga
    70981 cattagttga tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc
    71041 tgccaaacaa ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta
    71101 tgcattttcc aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag
    71161 agttcttttt atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga
    71221 atggtttgga gatcctttat gatgtccaaa aattgaaaca atttactcaa aagctacctt
    71281 ttgaactgac aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc
    71341 cgcagcatat gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg
    71401 cgattgcgtt atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag
    71461 aaatcttggc acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa
    71521 ccatcgcatt gattacgggg tcaacgaaag caaaagaacg acgtgagctc cttgaaaaac
    71581 tagagcaagg ggaaattaac gtcgtgatcg gcacacatgc attgatccaa gatggagtcg
    71641 aatttcagaa tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc
    71701 ggaaagtact tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga
    71761 ttcctagaac attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac
    71821 ttccagctgg acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg
    71881 tactggattg gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc
    71941 tgattgaaga atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc
    72001 gtgaactatt tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg
    72061 aaaaagaagt aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta
    72121 ctgtaattga agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg
    72181 atcgatttgg attggcacag ctgcaccaac ttcgtggacg tgttggacgc ggaagtgatg
    72241 cttcctactg tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa
    72301 tcatgacaga gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc
    72361 ctggtgaagt atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg
    72421 tgacggatgg aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcaaataa
    72481 aggattggca gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc
    72541 aggataatcg ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag
    72601 gagtggaaac aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa
    72661 ttgtagaagg cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg
    72721 gaaaagaagc agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata
    72781 cagacgaaaa aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg
    72841 catcaatggt ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag
    72901 cagggaatac aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata
    72961 tcgaacgccc tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg
    73021 atatgctgga tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta
    73081 ttttaggttc tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct
    73141 taaacaacgg aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc
    73201 ttcagaatga aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg
    73261 gtgtggctga tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg
    73321 aaggaacagc aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca
    73381 aaggaaaaat gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg
    73441 attattccaa acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc
    73501 atggagcaac aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg
    73561 agacagatgt agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg
    73621 atatctgaag cgatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa
    73681 aattagccat ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact
    73741 cgcgaagaag tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc
    73801 gataaagtga cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt
    73861 atggaattcg ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct
    73921 gaacaaattg aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt
    73981 caaataaaag caaagaagga taaaaagctg catgcagctt tttatccttc tttttttaat
    74041 ctttttgaca cgtaaaagtc atttttctga taattctggc gataaacaga aattatgttg
    74101 caaagtaaag cggttttcta tataattatt aataattcat tacacaaaga taagaataac
    74161 ctttgttata aaacgattta ttctgtgtaa tgtccttttt tattaagaaa aacttccatt
    74221 taggaagcag agaggggtat tcgattgtcc gataaccaat tattagaagt tcagaaccta
    74281 catacaggat tccgactcaa agatgactat tacgatgcag tagatgacgt ttcatttact
    74341 ttagacaaaa acgagatttt agcaatcgtt ggggaatctg ggtgcgggaa aagtaccttg
    74401 gctactacga ttatgggatt acttgatcct aataacacaa aaattacagg tgagatttta
    74461 tataacgatc tgaatttaat cgacctgaac gaaacgctct ataataaaat ccgcggaaat
    74521 gatatcggaa tgattttcca agatccgttg tctgcattaa atcctctcat gagaatcgaa
    74581 gaccagatca aagaaggact ttcctatcac acaaagatga atgcagaaca gcgtcaagct
    74641 cgtgcactag aattgctaga acaagtaggg attcctaatc ctgcgagagt tggacgccaa
    74701 tatccccatg aactatcagg cggtatgaga caacgtgtca tcatcgcaat cgcaatcgcc
    74761 tgcaaacctc caatcgtcat tgcagatgaa ccgacgacag ccctagatgt aaccatccaa
    74821 gcacagattc ttgatttact gaaagacctt caggaagaaa caaaaacagg gatcatcttg
    74881 atcactcatg acttaggagt cgtagcagaa atggctgatc gagtggcagt gatgtatggc
    74941 ggacagttcg ttgaagtagc aggtgtaaaa gaactgtttg atcatcctaa gcatccttat
    75001 acacgctctt tactgaactc tataccacaa gaaggaaatc atgatgcaga actccatgtg
    75061 atcgaaggtg tcgttccttc tctgaaaaac atgcctcgta aaggttgtcg gtttgcacca
    75121 cgtattccat ggataccagc aagcgatcat gaagaagagc ctatccttca tgaagtcgca
    75181 ccgaaccact tggtacgttg ctcctgctat aagcatttcc actttagaga cgaaaaagga
    75241 gaggtgtaga tgacagaatt aatcacaatc aaagacctga aagttcatta ccctattcgc
    75301 agtggattct tcaatcgagt aacggatcat gtatatgctg tagatggtgt ggattttatt
    75361 atcgaaaaag gaaagacata tggtttagtg ggagagtctg gctctggaaa atctactacg
    75421 ggaaaagccg ttgtgggatt agaaaaggta acagccggac aaatcatgta tgaaggaaaa
    75481 gatgtaacga agcgaagcaa ccgcaaaaaa atgggttata acaaggatgt gcagatgatc
    75541 ttccaagatt caatgtccag tttgaatcct aaaaagcgag tgttggatat cattgctgaa
    75601 cccatccgaa attttgaacg gttaagcgat caagaagaaa agaaaaaggt aaaaggactg
    75661 ctagatatcg ttgggatgcc ggaagatgcg ctatataaat atcctcatga attttcaggc
    75721 ggacaacgtc aacgtctagg agtagcccgt gctgttgcta ccaatccaaa actgatcgta
    75781 gccgatgaac ctgtatctgc tctggatcta tctgttcaag cgcaagtatt gaatttcatg
    75841 aaacgaatcc agcaagaata cggcctcagc tatttattca tttcacatga tttaggtgta
    75901 gtaaaacaca tgtgcgacaa tatcgcaatt atgtacaaag gtcgatttgt agaaatcggt
    75961 acaagagaag acatttataa tgacccgcga catatctata cgaaacgtct gctttcagct
    76021 attccgcaaa tcgatgtaga aaatagggaa agccacaaag aaaatcgacg aagagtagaa
    76081 caagagtata tccaaaacca aaaagaatac tatgatgcga ctggtcgagt ctacgatttg
    76141 cgtacgatca caactacaca caaagtggcg ttgaaagatg gaggtgcaag ctaatgtgga
    76201 aaacgattct gcgacgtgtt ttattgatga tcccgcaagt tattatttta agtgttctga
    76261 tcttcttatt agcccaagcg atgccggggg atccatttac cggtttgatc aatccaaatc
    76321 aggatccggc agtgatcgaa cagatgcgac aagcagctgg cttgaatgac ccatggtatg
    76381 tccagtactt ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa agtttcttat
    76441 acaaacgctc tgttgctgac ttgatcggcg aacggatcat caacacttta tatctatcga
    76501 tcttgacagt tgtccttact tatttgattg ctgtgcctct ggggatgctt gctggccggt
    76561 atcaaaattc aatcttggat aagattgtcg taatctacaa cttctttagt tttgctgttc
    76621 ctttatttat cttcggcttg atcatgctat ttgtttttgg ttatcgttta ggctggttcc
    76681 caacaagtgg atcacaaaca ctaggtttgc aaggtggatt tatcactcaa tggatagacc
    76741 gtttgaaata tattctgtta ccatcaatta cccaagcatt tttggcgact gccgtcacaa
    76801 tccaatatct gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc agaacagccc
    76861 gttccaaagg ggtaccgacg aataaagtat tcagtcgaca tattttccga aatgcagcgt
    76921 tgccgatggc ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt tctgtagtta
    76981 tcgaaaagat ttttggttat ccgggagtcg gaaaactatt tattgattcc atcggccaac
    77041 gggactatac cgtgatcaca tccttagtat tgattttagg aatcgcgacg ctagtgggga
    77101 cattactttc agatatcatc atgagtatcg ttgacccgcg tattcggatt caataaaacc
    77161 aatcagaaag gagaatggat cgatgagtga aaaaaacaat acagcagttc aagaaagtat
    77221 tcctcctatg gggatacgaa tgattgctcg agaatttaaa aaagataaaa ttgctatttt
    77281 ttcattaggt ttgctagtgg ttttattatt agtgatcttt atcggtgcga tgctgaccga
    77341 tcaagacaaa gtgatgtatg ttagtatctt cgacaaatac gctgcaccag gaacagtttc
    77401 gacacaagga caaaagttca ttctgggagc agatgaaggc ggacgtgatg tattaggaca
    77461 attgatcata ggtgctagaa actctgttac gatcggattt gcgatcacga tccttacttc
    77521 gatcatcggg gttggattag ggatcatctc tggctactac ggagggatca ttgacaacat
    77581 cttgatgcgg atcgttgatt ttatcatgat tctaccgatc atgctgatca ttatcgtctt
    77641 cgtatcgatt atttccagat acaatgtctg gtcctttata ttcatcatga gtgcatttta
    77701 ttgggtagct aaagcccgat tattccgaag caagacatta tctgaagcaa gaagagatta
    77761 tgtcagtgcc tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc gcgaaatcat
    77821 gccgaatcta agttcattaa tcattactaa tttgacgatg aactttgcgg caaacatcgg
    77881 tatcgagaca actttgactt tcttaggttt cggcttgccg gcaaatgtac ctagtttagg
    77941 tacattgatc ggttatgcaa gtaacggaga agtcttagtg aatcggcaat ggatctggtt
    78001 acccgcatca atccttattt tagttttgat gttgagtatc aattatgttg gacaagcatt
    78061 caagcgttct gcagatgcgc gccaacggtt aggctagtat agaagcgaag ggggaaatag
    78121 gaatgaagaa aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt cttgcagcat
    78181 gcgggggcgg aggtacagat acctctggaa acaactctgg aggcaacggc aataacaaag
    78241 aggaattaac ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa gacggcgaat
    78301 tagaagctgc ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg gagttttacc
    78361 aagattcata tgatgcagct tttatggctc cttctcatga accgctattt gcaaacgatg
    78421 acgatttcag aatcacagac gacggagctg caacgcttga tctagatgaa gatacgaaaa
    78481 aagcgacaat caaagtaaaa gacaacgtca aatggtcaga tggtgaagat gtaacggcgg
    78541 acgatgtgat tttcccttac gaaatcattg ggaataaaga ttacactggt atccgttatg
    78601 acgacacttt ccgtaacatc gtaggtatgg aagaatatca tgatggaagt gccgacacaa
    78661 tctccggaat caaaaaaata gacgacaaaa cagttgaaat cgaatataaa gaagtaaacc
    78721 caagcatgct tcaatcgggt ggtggtatct ggtcatatgc agcaccaaaa catacgttag
    78781 aaggtgtagc aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat ccagtgactt
    78841 ttggccctta ttacatgagt aatatcgttg caggagaatc agtagaatac ttgccaaacg
    78901 agcactactg gaatggtacg cctaaattga aaaagatcac gatgaaatct gtaccgatgg
    78961 caagtgcaac tgaagcattg aactctaaat tatatgatat gattttccaa atgccaacag
    79021 atacttacga tacctataaa gaaattgctg gctatacaaa cttaggccgt gaacaaactt
    79081 cttatactta tctaggattc aagttaggta aatggaatgc agatacaaag agtgtggaat
    79141 acaatccaga ttcaaaaatg gctgataaat cattacgtca agcaatgggc tatgcattag
    79201 ataataatgc agtaggtgaa cgtttctatg caggtttgag aagcaatgcg acttcattga
    79261 ttcctccagt attccagtca taccatgatg atgagatgaa aggattcaca caagatctag
    79321 acaaagcgaa aaaactatta gatgatgctg gctacaaaga tgtggatgac gatggtttaa
    79381 gagaagacaa agatggaaat aaactaacaa tcaactttgc ttcgatgtcc ggtggggaaa
    79441 cagcgcaacc attggcagat tactatatcc aacaatggaa agaaattggt ttggatgtcc
    79501 aatacacaac aggtcgtctg atcgaattca attctttcta tgataaatta gaaaacgatg
    79561 atcctgaaat tgatgtttac caagctgctt ggaatacagg aactgatcca aatccaagtg
    79621 gactatgggg accaaatgct gcgttcaact acactcgtta tgaatcagaa gaaaatacaa
    79681 aattgatcaa cgatatcagt tcaagcaaag catttgatga agagtaccaa aaagaagcat
    79741 atcaaaaatg gcaacaatat gcatttgaag aagcatttgc gattccaacg ttgttccgta
    79801 atgaagtatt gccagtcaac gaccgtgtca aagactggga ttggtcttat gatgtaccaa
    79861 acccatgggc aaatgtttct gttacagcag attcacgttc ataatcacta aaaatgaccg
    79921 aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc tctaatattc
    79981 aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc cagtttctcg
    80041 gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt ttttcttcat
    80101 actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta ttaaagcttt
    80161 acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat aaggaagtat
    80221 cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa aagaactaaa
    80281 agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt ttacccattc
    80341 atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac ttgaattttt
    80401 aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat atcctcattt
    80461 gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca gcttgtcgaa
    80521 atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag aagagaattc
    80581 aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt taggcgcgtt
    80641 atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga tttttcctaa
    80701 agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac aagaagtctt
    80761 gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag gtccagctca
    80821 tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca ctggacaagg
    80881 gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag cactgtaaaa
    80941 gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt aaatcatttg
    81001 cagacaagac agtcattgat tttgaaaata gcgtcacagc agtggttgga ccaaatggca
    81061 gcggaaaaag caatattaca gaggctatcc gctgggtatt aggagaacaa tctgctaaaa
    81121 gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc cgtaagcagc
    81181 tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg ccattggagt
    81241 acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag tttttcatca
    81301 ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagattct ggattaggaa
    81361 aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt agtaaaccgg
    81421 aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag cagcggaaaa
    81481 agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc caagacatca
    81541 tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca gcaaaggagt
    81601 ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt gctgaaatca
    81661 agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc aatctagaat
    81721 taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag cagcgtaaag
    81781 aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta gatctttcgg
    81841 aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca aagcatacac
    81901 aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag gttaaacatt
    81961 ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca gagatccaaa
    82021 aagctgaagc aaatctcata aaaacacagc aagaattaga aaaataccaa aaatcaacga
    82081 aagaattatt ggcagaatta agagatcaat atgttgattt gatgcaagaa caagcagctg
    82141 taggaaacga actgaagtat ctagaacgtc aatatataca agaaacagca aaaagcaagc
    82201 agacgttagc caagcaatcg gaagtagaag catcagtaga ccgattgatc ttacaaaaag
    82261 aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca aaagagaagt
    82321 tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa gaacagccta
    82381 aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa agccttcaag
    82441 aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg cagcataaac
    82501 agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct gcagatttca
    82561 cgttagctat cgaaacagct ttaggtgggg cagcccagca tgtcatcgtt gaaaatgaaa
    82621 aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga gcaacattcc
    82681 ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca caagcagcag
    82741 ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccgat caaatccaaa
    82801 ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact agtgccaatg
    82861 caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt gatgtgatga
    82921 atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc agtttgtttg
    82981 tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat aaacaactcc
    83041 aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta gccgaagaac
    83101 aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa gcgacaaatc
    83161 agctacaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa atctcaactt
    83221 ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg aacgaattga
    83281 aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa atcaaatcgt
    83341 taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt caaaaagcac
    83401 aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag atccagctcc
    83461 gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata gaaaagcagt
    83521 tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt ttggaaaaac
    83581 aaattaacga attatctgct cagcgagaaa cattaaaagc cgagttggca aaagccaaag
    83641 agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg gcagagcgta
    83701 atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa aaagaccgag
    83761 ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag ctgacatttg
    83821 agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga acaaaggttt
    83881 catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca atcgaacaat
    83941 acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat ctgttagcag
    84001 cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg acaagattca
    84061 aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat atgtttggcg
    84121 gcggtaaagc tgaattagta ctgacggaac caactgattt gctgaaaaca ggtattgaga
    84181 ttgaagtaca acctcctggc aaaaaactgc aaagtctaag tttgctttct ggaggagaac
    84241 gagcacttac agccatcgcg ttgctctttt ccattatccg tgtctgtcct gttccgtttt
    84301 gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt tttggtcgtt
    84361 acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc aaaggaacga
    84421 tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta tcaaaaatcg
    84481 tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg aaagtgtagg
    84541 aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa atgaacaaaa
    84601 gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag gggtcaagat
    84661 cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag aattaggact
    84721 ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa aaaatgatac
    84781 tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct tatacaaact
    84841 ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt tacagctgcc
    84901 tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc aattccaacc
    84961 agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa tcgcttatga
    85021 ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg aaatgtatga
    85081 gatcatcaaa acaagaagta tgcttttgga atttatgcca aaaggtgtta caaaagctta
    85141 tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca tggcgatcgg
    85201 tgatgaggaa aatgatttac cgatgatcca atatgctgga ctaggcgtag cgatggagaa
    85261 tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg aagaaaatgg
    85321 cgtagccaaa gtgatccaaa aatttgtatt agagtagggg gagtcaaatg ggattttttg
    85381 ataaaatcaa gcgtgctttt actggcgaag agaaaacaga agaagttgaa gtacaggaga
    85441 agtacgacaa gggtttagaa aagacgcgta aaacatttgg tgaacggatg aatgagttgt
    85501 ttgccaattt tcgaacagtc gatgaagact tttttgaaga attagaagaa acacttatcg
    85561 gtgcagatgt aggttttgaa acagcgatac gaattaccga agcattacgt caagaagtga
    85621 aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag aaacttgtgg
    85681 atctctatga agcggaagga atcaatgaag taaatgaact aaatatccaa gaagacggac
    85741 taacagttat tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt attggtaaac
    85801 ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca gataccttcc
    85861 gcgctggagc aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt gaagttgttc
    85921 gtggcaatgc aggaggcgat ccagcagctg tcgtattcga tgctttggaa agagcaaaga
    85981 cagagcaggc agatgtttta ctagttgata ctgcaggccg actgcaaaat aaagtgaact
    86041 tgatgaacga attagaaaaa ataaaacgag taatccagcg ggaattacca gaagcacccc
    86101 aagaaacact tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt caagcaaaac
    86161 aattcaaaga aacaacagat gtgaccggtc ttgttttgac aaaattagat ggtacagcaa
    86221 agggcggtat cgtacttgct atccgtaatg aattgcattt accagtgaaa ttggtcggtc
    86281 ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa tgattttgtc gttggattgt
    86341 tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa gcaggagctg tgacaaaagt
    86401 tgcagctcct tgtttgaatg attgttttta acatagctgt gtttttgatg ccttcatttt
    86461 tatacttatt tgtaagcggt tctcaaacaa tttcgttctt tttcttagac tttttgatta
    86521 taattagatt catatgaatt ggaggtcact taatgatgaa ttttaagcaa ttattattgc
    86581 atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg tatagaacgg gaaggacagc
    86641 gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt ggcgatcgat
    86701 cctatcaccc ctatatccaa acagatttta gcgaaacaca aacagagatg atcacccctg
    86761 ttaccgattc tattcccgaa ttatttcagt atctcgctgc tgtttatgat gtgactgctc
    86821 gttctatacc gaaagaagag atgatctggc cattaagtat gccacctgcc ttaccggaaa
    86881 aagacgaaga gatcattatt gcaaaattaa aaaatttcga agatgtcttg tatcgacgtt
    86941 atttagcaaa agaatacggg aagcgaaaac agatggtaag cggtattcat ttcaattttg
    87001 aatttggcga tgagttgcta agaacattgt tcagccatca ggaagaattt caagattttt
    87061 ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc tatcgctgga
    87121 tgatcactta tttattcggt gcttcaccaa tgagtgagaa gaactacttt ttagatgaat
    87181 cacatccgca agaacctgtt cgcagtattc gcaacagtgc gctgggctat acgaatcatc
    87241 caaatgtgaa agtttcctat gcttctatga aacagtattt agcagatatc gagcgaatga
    87301 tcgaagaagg gaaactttcg gaggagaaag aattttatac accgcttcgt ttccgaggcg
    87361 gaaagaaagt cgcagattta gcaacaacag gtgttcgtta tattgaattg cggaatatcg
    87421 atttgaatcc ttatgcaaga ttgggtatca atccagagca agttcggttc ttacaattat
    87481 tcctgatgta catgttatgg acagaagaaa aagaagactg tgaccaatgg gtagcagaag
    87541 gtacgactcg aaataacaaa gtagcactag aacaaccatc agatcaaaca gaatttcatc
    87601 aagaaggtag agaaattctt gaaggaatga agcaaatgct ggttgaattg gattggttgg
    87661 attctcttta tttagtagag gaagcattga ctcaaatgga tcatcctgaa caaacgttag
    87721 cagcgaaact ctatcaagaa gcacagctat cgagtcagca agaagttgct gtggcattgg
    87781 gacatcaata ttataaagaa agccatgaac gtccctatca gttggccggt tttcgcgaaa
    87841 tggaactttc tactcagata tttatgttcg atgccatcca aaaaggtgtc caagtcaaag
    87901 tattagatga gtcggatcaa tttttgcgtc tgcaattcca agaccatgta gaatatgtga
    87961 aaaatgcaaa catgacaagt aaggacagct atatcgtccc acttatcatg gaaaacaaaa
    88021 cagtaacgaa aaaagtctta aaagaagctg ggttccgggt accaggaggc gcagaatttt
    88081 catccatgga agaagcggta aaagcttatc cgagatttgc cgagcaggca tttgtgatca
    88141 aacctaaatc aaccaattac gggttaggta tcacgatttt caaggaagga gctagtctgg
    88201 aagattatca agcaggttta gccattgctt ttcgtgaaga cagttcggtt ttagtagaag
    88261 aatttatgcc gggaacggaa tatcgtttct ttgtgatcga cggagaagtc caagccatca
    88321 tgttgcgagt ccctgctaat gttattggtg acagtatccg aacagtgaaa gaacttgttg
    88381 aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta gaattgatcc
    88441 aattaggtga attagaacaa ttgatgttga aagaacaagg gttgacgatc gaatcagttc
    88501 cccaagccaa tcaaattgtt tatctaaggg agaactcaaa tatcagtaca ggtggtgatt
    88561 cgatcgatat gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt tctgcggtag
    88621 aagcactagg agctaagatt agtggtatcg atttgattat accagataaa gaaatcgatc
    88681 ctacaactga taaaaaagca tatgggatca tcgaagcaaa tttcaatcca gccatgcata
    88741 tgcatgttta tccttttgca ggaaaaggca gaagattgac gatgaatgta ttgaaattgt
    88801 tatatcctga agtattttaa aagaaaaagc ttttgacaga ttgtcctatt tgatgtcaat
    88861 ctgatcaaaa gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt aaaataaacg
    88921 atcccagaag ctttttttct tttttggttt ttctttttca gctggcgaag agttttcttc
    88981 tgggtatttt ttttcgatat caatcacagg ttcatttact gcttcttttg ctgttaagag
    89041 caatccgaat tcctcaggat ttgcagtgac gtaatcgttg actacagtga aacggctatt
    89101 gtttttcgtt gctagctgga tatattggtt ttggacagaa aggggtagag ccccattcaa
    89161 gagtatggaa gcatctggat gttttttgag ttcttttaaa aagttttctt tgttttttgg
    89221 ttggatcatt tcagcaacag tcatactaag atagcagcgt tccctgaatg tccctaaata
    89281 tttatgctgt tcttcagggt tcactaaagg tgtaccataa agtcccttat ccaaatggct
    89341 ttttacatca tcttgtgcca taaaaaatac cctccttctt ctctttgtct ttcttacgat
    89401 tatagcatat tcccaataat acctaaaaca tatgaaaaag cggttgcttt tgttcaacga
    89461 ttcacttaaa ataagaatga aaatcattct caattagaaa ggtggattgg atgcgctacg
    89521 gacgactagt agcattatta gttattttaa gtagtatttc attgttcatc ggagtatcga
    89581 atgtacaatt gtcagaactg ctccagttag atgggcaaag cttacaaatc ttgctagtga
    89641 ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt atttgtgggt
    89701 tgatcatgca acatttgacc caaaataaat ttgtttcacc aacgactgcg ggcacgatgg
    89761 atagtgcgcg tttaggtatt ttagtggcta tgattttcct acctggtgca tccttgattg
    89821 tccgatcctt gacagcattt tgctttgctt ttgcaggaac attgcttttt ttatccctta
    89881 cgcgattgtt cctccagaaa aatcaattga tgcttccgtt gattggagtc atgttaggga
    89941 atatcattgg ttcagttgct actttttttg cttatcaatt ccagttggta caaaatatga
    90001 cttcatggct tcaagggaat tttgcgactg tgatacgagg aaattatgag ttattgtatg
    90061 ctgttgttcc attgttgctc ttggtttggc tatttgctta ccgatttacg atcattggtc
    90121 tgggcgaatc gtttgcagta aatctgggaa tagattacca gaagatgcag tttttaggca
    90181 ttggcttagt atcattggct agtgcagtca tgttgatcat ggtaggaagc attcctttta
    90241 tcggagtgat cattccgaat cttgtctcta gaatatatgg agatcaagtg cacaaaacaa
    90301 ttggcgtaac tgctttagca ggtagtttgt tcttagtatt ttgcgatctt gtcgctcgcg
    90361 tgttgatttt tccttatgaa ataccagtca gtgtgatcgt tggaatcatc ggtggggcta
    90421 tcttccttta tcttctgata aggggacgac gatatgctta aaaaaaagcc atctattttt
    90481 attggtatgc ttgttcttgt tttatttgtt acaggaatgt acttgacgat ccatacatat
    90541 ggaaactggc attttgcatg tgcattgagg agtaagaaaa tttttgcatt tatccttgtc
    90601 tcgctagctg tcagcagtgc cacaatcagc tttcagacaa ttacgcataa ccagtttttg
    90661 actccttcag tattgggact tgagtcgtta tatgtccttg tccaaacggt ccttttcttc
    90721 tttatcggag gagtgacgat gcttcagcag acaggtacag tttttttctt attgaacgtt
    90781 gggctgatgg tcgggctgag tctattgtta tttttctttc ttttgaaaaa aatgagagcg
    90841 aatctttttt tattactgat gtcagggatg attttaggta cgctttttgg tagtgtcagt
    90901 acctttttac aagtcatcat ggatccgaat gaatatgatt tgcttcaagg cagattattt
    90961 gcaagctttg gcaatatcca gagtcagtat cttttgcctg cttctttgat cattatcggc
    91021 tgctggtacc tgttatttcg atatcgacat gtgctagacg ttttccatct cggaaatgaa
    91081 caagcagtaa acctcgggat agacacagat cgttttgaat ttttgatttt atgttgtgtc
    91141 agtcttttga cagggacatc aacggctctt gtgggtccag ttactttttt agggtttatt
    91201 gtcgcaaata tcagttatcg tgtcttttct acttatcgtc atggtcagct atttatcgga
    91261 agctttctgg tagctatgct gtttctgaca ggaggacagc tcgcagtaga gcagattttc
    91321 ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta ttttgtcaca
    91381 aaattgattc gcgaaaggaa gagtatccta tgattgattt acaaaaggtc aataaactgt
    91441 accatggaaa atcagtggtt aaagatgtac agttaacgat tcctgaaaag aaattgactg
    91501 cttttatcgg tccaaatggt gctggaaagt caacggtgtt gtcgatgatc agtcgtttga
    91561 taccaaaaga tacaggtgaa atctatttag accataacga agtaaaggct tggaaatcca
    91621 atgaattagc caaaaaatta tcgatcctac gacaatcgaa tgaaataaat gtccaaatca
    91681 ccgtgaaaga attagtagaa tttggacgat ttccttatac gaaaggaaaa ttgacgacaa
    91741 aagacgagga aatcgttcag gaatcactgg aacaccttgg gttagaagaa atagcggatc
    91801 aatcgattga cactctttca ggcggtcaac tccaacgtgc gtatattgca atggtgctag
    91861 cgcaagacac ggattatatt ttactggatg aacctttgaa caatctagat atgaattttg
    91921 cggttcaaat catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa acaattgtta
    91981 ttgttcttca tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt gcaatgaaag
    92041 atggacagtt atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat gtattggatg
    92101 aattatacga aatggacata cggatttgtg agattgaagg ccgcagattc tgtctttatt
    92161 tctaaaacta acattaggag agaagagatg aaaaaattag tagtaggtat cttgattcta
    92221 gcatcatttg gtttagcagc atgtggaaat agcaacaaca ccagtcaggc agacacaaaa
    92281 aaaagcagca cacaaactga aactacattg acgataacag atagtaatgg agaccaaatc
    92341 gaggtgccaa ataatccaga aaaagtcgtc gtattcgaca atggttcttt agatacgatg
    92401 gatgctcttg gtgttggaga taaggtagtt ggagcagcta caagcagcct tcctgagtat
    92461 ctttcatcct ataaaaaagt agaatcagca ggtggaataa aagaaccaga cttagaaaaa
    92521 attaatcagc ttcagccaga tctaatcatc atttctggcc gacaaagaga ttttcaaaaa
    92581 gatctaagtc aaattgctcc gaccattttc ctttctttag atgcgaaaaa tccatgggaa
    92641 tctttccagc aaaatgtcac tgctttaggg gagatctttg gtaagcaaga agaagctaaa
    92701 actcaattag aggaattatc tagtgcaatc gatcagacga aaaagaaagc agaagctact
    92761 gacaaaaaag cactagtcac acttgtgaat gaagggcaat tatcagccta cggatctggt
    92821 tctagattcg gtttcatcca tgatttattt ggctttgaac aagcagatga tcaaatcgaa
    92881 gcatccactc acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa tccggatatc
    92941 ttatttgtag tagacagaac aaaagctatt ggtggagacg acagcaaaga tgatatttct
    93001 gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc agcagatcat ctcccttgaa
    93061 ccagatgtct ggtatttaag cgggggcggt ttagaatcaa tgaaactgat gatcgaagat
    93121 gtgaatcaag catttaaata gcataaataa cagaggatta aactctcata ctatgtaaca
    93181 ataatgtttc ttacattctt aaatagcaag agcctatcct caaagaagtt atcaaaaacc
    93241 atccgtaaaa tcgtattgat gattttacgg atggttttta tttatttgag aaatcttttt
    93301 tcaacgtgaa ttcattatct acatcatacg ggttgattct agaatgggga gtggcaaaaa
    93361 atagtgtgat ccattttttc agcatcaaga tcgcctcctt tgatttgaat caaaggtaca
    93421 aagcgaaact tagataaaac ttaaatttag atatttttat tttgtaccga tctcttcttt
    93481 aaacgatgtt ccatctgctg cttctcggca ataagtccac tagactagaa aatatgagaa
    93541 gctattttct gtccagcgtt cttattactc aaagcaaatc gcagttgtca caatctcttc
    93601 taaacgatgt tccatctgct gcttctcggc aataagccca ctagactaga aaatatgaga
    93661 agctattttc tgtccagcgt tcttattact caaagcaaat cgcagttgtc acaatctctt
    93721 ctaaacaatg ttcaatcagc tgaccttcgg tattagctca aagcactgat ttcacaacct
    93781 ctatttaggc atgtgtctca gcaagcggtt gatagttgat gatctgcatt tttcgataaa
    93841 aatcggcggg aagcttactc caccgtttta ataattgata gaaataactc tcttctgctt
    93901 tgattttata ctcgcttaat atcaatccaa aactattcgt tttttttgaa gctaattttt
    93961 tataaatatc caatgctttg gttttatctt cattgacaaa gagtgttttt acttcaatat
    94021 aagaaccctc gatattttgt gtttcttcga taaaatagat gttttttata tattccttca
    94081 tagtgattcc tccaaagaag tcttttattt gataagttta ctctaccgaa gagttgttaa
    94141 taatccgtga acaaaaagta attctttttc caatagttgt taaaggtttt ttatttttta
    94201 tagtattttt tgttatttaa gaaaggaata aggttaaaca aataacaggt tgctttttgg
    94261 gtctttttcc gttcaagaaa ctgaacgaag aaaagggaaa gttgtctctt ttgtgataag
    94321 agaaatcata ataaacgaat gacttacttt ccaataaatt gagaacagtc agaaccagac
    94381 aaattagttg catctgatga ataaagtgat tattctatga catgaggtga tacagatgaa
    94441 attagaagtg acaaaagaag cacaagaagt attgaagaat aaattagaag cagacgatca
    94501 acttttatta gatatcgaaa acggtgacgg tccatttgca gaaagccaag tcagttgcca
    94561 attggatacg gcatttcgat tgatcattgt caaaaaagat acacaaacag atctaagtct
    94621 ttattctgta gtggctgata caccagttgg accaatcaaa atcaaagaca gtgcaatact
    94681 ttacatggat gatccatcca cacttgcttt agaaccaact tataatagtt tacaattaaa
    94741 aggaccaagc ggcttgagaa aaggcaactt gcaagttgtt cgaaaagact agagggataa
    94801 aatgggaaaa ttggcgatca tcagcgacct acatgtggac atcaataaat tcggcgaatc
    94861 agagctgatg cagctttggc aagttttgca aaagcacaag atcactcggc ttcatctagc
    94921 cggagatacg gctaataaag tagaccgttg cctagctgtt gtcgaatttt tcgtcaataa
    94981 aggattatct acgacatttc atttcggcaa tcacgagttg gcagatgtgc ccaatgaaca
    95041 gatgatggac cattttccag acccacattt tttaaatgaa cggtatattg ctttaaatga
    95101 gcagaccgtt cttttaggga tgaatggctg gtacgactat cagttttccg aattgaaaga
    95161 ttacgaggca atcgtacgaa tgaagcgttt gtattggtat gatcggttca tccagcgtgg
    95221 gaaaacagat ctcgaggtca atgcagaaat gcttgcagct gcaaaaaatt tgctagacca
    95281 gctggaagca aaaaaacttg aggtgatttt atccactcat ttcgttccta aacgggcatt
    95341 tatcatgtat caatctgctc cttatgagcg ttggaacaaa ttaaatgcat ttttaggatc
    95401 agaatcattc ggagaattac ttgaccgtta ttcaaatatc aaacaagtag tttttggcca
    95461 tacgcatcgt cgctttgaag ataaatccat cggtcacacg atttacagct gcagaccttt
    95521 tggttattat tatgaatggc agctgacccg agattttgtc ttgaaagaac agttgctaga
    95581 cagctatgat cctatgaaat tacgtgtcct cttgcgtgcc catcacccag cttttcaggc
    95641 ctatcaaaca caacatttgc aagaggaatt tgaacaagcg atgacaatcg ttccgtattg
    95701 agacagcatt agtaatgtgt aaaagaaaat accttgtaaa acataaggat aagtaagagc
    95761 agtagatcca tttatcgtgg atatgctgct ttttttgcgg aaaataatca gcgaatgttt
    95821 atttgatcgg atgatttgta actcgcagag tactagatgc aagcgtgtat tctttttttg
    95881 aacgataatc acaaaaaaaa atata
//
LOCUS       DANJLF010000003        89177 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000003 DANJLF010000000
VERSION     DANJLF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(79..1692)
                     /locus_tag="PWT25_000220"
     CDS             complement(79..1692)
                     /locus_tag="PWT25_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1020336.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(1833..2741)
                     /locus_tag="PWT25_000221"
     CDS             complement(1833..2741)
                     /locus_tag="PWT25_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1020337.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            2924..4048
                     /locus_tag="PWT25_000222"
     CDS             2924..4048
                     /locus_tag="PWT25_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1020338.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4275..5192
                     /locus_tag="PWT25_000223"
     CDS             4275..5192
                     /locus_tag="PWT25_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1020339.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5185..6018
                     /locus_tag="PWT25_000224"
     CDS             5185..6018
                     /locus_tag="PWT25_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1020340.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6101..7219
                     /locus_tag="PWT25_000225"
     CDS             6101..7219
                     /locus_tag="PWT25_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL1020341.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7213..9111
                     /locus_tag="PWT25_000226"
     CDS             7213..9111
                     /locus_tag="PWT25_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL1020342.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9105..10328
                     /locus_tag="PWT25_000227"
     CDS             9105..10328
                     /locus_tag="PWT25_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL1020343.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10414..12348)
                     /locus_tag="PWT25_000228"
     CDS             complement(10414..12348)
                     /locus_tag="PWT25_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1020344.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            12574..13230
                     /locus_tag="PWT25_000229"
     CDS             12574..13230
                     /locus_tag="PWT25_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL1020345.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13304..13657)
                     /locus_tag="PWT25_000230"
     CDS             complement(13304..13657)
                     /locus_tag="PWT25_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL1020346.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            13849..14778
                     /locus_tag="PWT25_000231"
     CDS             13849..14778
                     /locus_tag="PWT25_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL1020347.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14789..15625
                     /locus_tag="PWT25_000232"
     CDS             14789..15625
                     /locus_tag="PWT25_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL1020348.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            15873..16649
                     /locus_tag="PWT25_000233"
     CDS             15873..16649
                     /locus_tag="PWT25_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1020349.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            16825..17568
                     /locus_tag="PWT25_000234"
     CDS             16825..17568
                     /locus_tag="PWT25_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020350.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            17561..19141
                     /locus_tag="PWT25_000235"
     CDS             17561..19141
                     /locus_tag="PWT25_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL1020351.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19392..19871)
                     /locus_tag="PWT25_000236"
     CDS             complement(19392..19871)
                     /locus_tag="PWT25_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL1020352.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19887..20639)
                     /locus_tag="PWT25_000237"
     CDS             complement(19887..20639)
                     /locus_tag="PWT25_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL1020353.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            20756..20826
                     /locus_tag="PWT25_000238"
     tRNA            20756..20826
                     /locus_tag="PWT25_000238"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20788..20790,aa:Gly,seq:ccc)
     gene            21187..21912
                     /locus_tag="PWT25_000239"
     CDS             21187..21912
                     /locus_tag="PWT25_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL1020354.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22167..22433
                     /locus_tag="PWT25_000240"
     CDS             22167..22433
                     /locus_tag="PWT25_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL1020355.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22430..22861
                     /gene="ruvX"
                     /locus_tag="PWT25_000241"
     CDS             22430..22861
                     /gene="ruvX"
                     /locus_tag="PWT25_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL1020356.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            22886..23191
                     /locus_tag="PWT25_000242"
     CDS             22886..23191
                     /locus_tag="PWT25_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL1020357.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23272..24417)
                     /locus_tag="PWT25_000243"
     CDS             complement(23272..24417)
                     /locus_tag="PWT25_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1020358.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24478..25125)
                     /locus_tag="PWT25_000244"
     CDS             complement(24478..25125)
                     /locus_tag="PWT25_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1020359.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25319..25612
                     /locus_tag="PWT25_000245"
     CDS             25319..25612
                     /locus_tag="PWT25_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020360.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            25656..25967
                     /locus_tag="PWT25_000246"
     CDS             25656..25967
                     /locus_tag="PWT25_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020361.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            25964..26588
                     /locus_tag="PWT25_000247"
                     /pseudo
     CDS             25964..26588
                     /locus_tag="PWT25_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            26729..26887
                     /locus_tag="PWT25_000248"
     CDS             26729..26887
                     /locus_tag="PWT25_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020362.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            26888..27250
                     /locus_tag="PWT25_000249"
     CDS             26888..27250
                     /locus_tag="PWT25_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020363.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27287..28135
                     /locus_tag="PWT25_000250"
     CDS             27287..28135
                     /locus_tag="PWT25_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL1020364.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28125..29600
                     /locus_tag="PWT25_000251"
     CDS             28125..29600
                     /locus_tag="PWT25_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL1020365.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            29866..30273
                     /locus_tag="PWT25_000252"
     CDS             29866..30273
                     /locus_tag="PWT25_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL1020366.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30276..30491
                     /locus_tag="PWT25_000253"
     CDS             30276..30491
                     /locus_tag="PWT25_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020367.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30495..30875
                     /locus_tag="PWT25_000254"
     CDS             30495..30875
                     /locus_tag="PWT25_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL1020368.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31009..31164
                     /locus_tag="PWT25_000255"
     CDS             31009..31164
                     /locus_tag="PWT25_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL1020369.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31233..31706
                     /locus_tag="PWT25_000256"
     CDS             31233..31706
                     /locus_tag="PWT25_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL1020370.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            31703..33397
                     /locus_tag="PWT25_000257"
     CDS             31703..33397
                     /locus_tag="PWT25_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL1020371.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33363..33548
                     /locus_tag="PWT25_000258"
     CDS             33363..33548
                     /locus_tag="PWT25_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020372.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            33552..34727
                     /locus_tag="PWT25_000259"
     CDS             33552..34727
                     /locus_tag="PWT25_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL1020373.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            34720..36243
                     /locus_tag="PWT25_000260"
     CDS             34720..36243
                     /locus_tag="PWT25_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL1020374.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36299..36583
                     /locus_tag="PWT25_000261"
     CDS             36299..36583
                     /locus_tag="PWT25_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL1020375.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            36570..36905
                     /locus_tag="PWT25_000262"
     CDS             36570..36905
                     /locus_tag="PWT25_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL1020376.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37200..37826
                     /locus_tag="PWT25_000263"
     CDS             37200..37826
                     /locus_tag="PWT25_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020377.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            38568..39405
                     /locus_tag="PWT25_000264"
                     /pseudo
     CDS             38568..39405
                     /locus_tag="PWT25_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            39470..39904
                     /locus_tag="PWT25_000265"
     CDS             39470..39904
                     /locus_tag="PWT25_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1020378.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(39972..41294)
                     /locus_tag="PWT25_000266"
     CDS             complement(39972..41294)
                     /locus_tag="PWT25_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL1020379.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41448..43088)
                     /locus_tag="PWT25_000267"
     CDS             complement(41448..43088)
                     /locus_tag="PWT25_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL1020380.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43418..43888
                     /locus_tag="PWT25_000268"
     CDS             43418..43888
                     /locus_tag="PWT25_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL1020381.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44517..45620
                     /locus_tag="PWT25_000269"
     CDS             44517..45620
                     /locus_tag="PWT25_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL1020382.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45904..46584
                     /locus_tag="PWT25_000270"
     CDS             45904..46584
                     /locus_tag="PWT25_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL1020383.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46674..47708
                     /locus_tag="PWT25_000271"
     CDS             46674..47708
                     /locus_tag="PWT25_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL1020384.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47722..48057
                     /locus_tag="PWT25_000272"
     CDS             47722..48057
                     /locus_tag="PWT25_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020385.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48041..50521
                     /locus_tag="PWT25_000273"
     CDS             48041..50521
                     /locus_tag="PWT25_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020386.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            50577..52136
                     /locus_tag="PWT25_000274"
     CDS             50577..52136
                     /locus_tag="PWT25_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020387.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52133..53497
                     /locus_tag="PWT25_000275"
     CDS             52133..53497
                     /locus_tag="PWT25_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1020388.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53668..54738
                     /locus_tag="PWT25_000276"
     CDS             53668..54738
                     /locus_tag="PWT25_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020389.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54753..55421
                     /locus_tag="PWT25_000277"
     CDS             54753..55421
                     /locus_tag="PWT25_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020390.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55441..56019
                     /locus_tag="PWT25_000278"
     CDS             55441..56019
                     /locus_tag="PWT25_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1020391.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56422..57333
                     /locus_tag="PWT25_000279"
     CDS             56422..57333
                     /locus_tag="PWT25_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1020392.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57589..58506)
                     /locus_tag="PWT25_000280"
     CDS             complement(57589..58506)
                     /locus_tag="PWT25_000280"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL1020393.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58708..59145
                     /gene="zapA"
                     /locus_tag="PWT25_000281"
     CDS             58708..59145
                     /gene="zapA"
                     /locus_tag="PWT25_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL1020394.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59203..59750
                     /locus_tag="PWT25_000282"
                     /pseudo
     CDS             59203..59750
                     /locus_tag="PWT25_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            59865..62225
                     /locus_tag="PWT25_000283"
     CDS             59865..62225
                     /locus_tag="PWT25_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL1020395.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62312..62626
                     /gene="trxA"
                     /locus_tag="PWT25_000284"
     CDS             62312..62626
                     /gene="trxA"
                     /locus_tag="PWT25_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL1020396.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62808..64586
                     /gene="uvrC"
                     /locus_tag="PWT25_000285"
     CDS             62808..64586
                     /gene="uvrC"
                     /locus_tag="PWT25_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL1020397.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64731..65630
                     /locus_tag="PWT25_000286"
     CDS             64731..65630
                     /locus_tag="PWT25_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020398.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65623..66885
                     /locus_tag="PWT25_000287"
     CDS             65623..66885
                     /locus_tag="PWT25_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020399.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67048..68946
                     /locus_tag="PWT25_000288"
     CDS             67048..68946
                     /locus_tag="PWT25_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL1020400.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69245..69964)
                     /locus_tag="PWT25_000289"
     CDS             complement(69245..69964)
                     /locus_tag="PWT25_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL1020401.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            70301..70633
                     /locus_tag="PWT25_000290"
     CDS             70301..70633
                     /locus_tag="PWT25_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020402.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70620..72623
                     /locus_tag="PWT25_000291"
     CDS             70620..72623
                     /locus_tag="PWT25_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL1020403.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72642..73112
                     /locus_tag="PWT25_000292"
     CDS             72642..73112
                     /locus_tag="PWT25_000292"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL1020404.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73133..73717
                     /locus_tag="PWT25_000293"
     CDS             73133..73717
                     /locus_tag="PWT25_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL1020405.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73732..74718
                     /locus_tag="PWT25_000294"
     CDS             73732..74718
                     /locus_tag="PWT25_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL1020406.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            74708..75019
                     /locus_tag="PWT25_000295"
     CDS             74708..75019
                     /locus_tag="PWT25_000295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL1020407.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75041..76822
                     /locus_tag="PWT25_000296"
     CDS             75041..76822
                     /locus_tag="PWT25_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL1020408.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            76815..78191
                     /locus_tag="PWT25_000297"
     CDS             76815..78191
                     /locus_tag="PWT25_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL1020409.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78198..78833
                     /locus_tag="PWT25_000298"
     CDS             78198..78833
                     /locus_tag="PWT25_000298"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL1020410.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            78863..80218
                     /locus_tag="PWT25_000299"
     CDS             78863..80218
                     /locus_tag="PWT25_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL1020411.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80410..81804)
                     /locus_tag="PWT25_000300"
     CDS             complement(80410..81804)
                     /locus_tag="PWT25_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL1020412.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            81896..82867
                     /locus_tag="PWT25_000301"
     CDS             81896..82867
                     /locus_tag="PWT25_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL1020413.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            82864..83646
                     /locus_tag="PWT25_000302"
     CDS             82864..83646
                     /locus_tag="PWT25_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL1020414.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(83815..84186)
                     /locus_tag="PWT25_000303"
     CDS             complement(83815..84186)
                     /locus_tag="PWT25_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1020415.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84320..84763
                     /locus_tag="PWT25_000304"
     CDS             84320..84763
                     /locus_tag="PWT25_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL1020416.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(84806..85615)
                     /gene="yidA"
                     /locus_tag="PWT25_000305"
     CDS             complement(84806..85615)
                     /gene="yidA"
                     /locus_tag="PWT25_000305"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL1020417.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(85634..87001)
                     /locus_tag="PWT25_000306"
     CDS             complement(85634..87001)
                     /locus_tag="PWT25_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1020418.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(86985..87815)
                     /locus_tag="PWT25_000307"
     CDS             complement(86985..87815)
                     /locus_tag="PWT25_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL1020419.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            87978..88415
                     /locus_tag="PWT25_000308"
     CDS             87978..88415
                     /locus_tag="PWT25_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL1020420.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88491..89111
                     /locus_tag="PWT25_000309"
     CDS             88491..89111
                     /locus_tag="PWT25_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL1020421.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        29363 a        15101 c        18395 g        26318 t
ORIGIN      
        1 aaccctatat actttttcat ttttactcct caagtaaaaa taactcctag accgcggcta
       61 cccaccgcgg tcgttttttt attcttcaat caaaatcgct ttgataccca aacctgccaa
      121 gattcgggca gaagtataag tattcaatcc tcttctgaaa gtaatgtaaa cttttttatc
      181 ttttgggatt tcattgattc gatctcgtaa ctcattcatt ggaatatttt ttgttgccga
      241 gatactgcca gaaggtgctt tattaggttc acgtatatct aaaaagaacg cttctttcaa
      301 atcgttttcg ggtatatcac ttgctttgat cgtttctacg atattcgtca tttgattgat
      361 tgcgacataa ccagctatat tcaacgggtc acgggtagta gaatacggtg gagagtaagg
      421 caactccaaa tcaggcaaat caaagactgt caagtttccg gtgattgcta cagaaagttc
      481 tcccatccgt ttatcgacac ctttttctcc aaatgcttgt ccacctaaaa tacgaccgct
      541 atctgcatca aaaatcagct ttagatttaa tcttgtagct tctggataga aataggcatg
      601 gtcaaaggga gtgataaaaa cagttttata atttgttatg cctgatgctt ttaattcatg
      661 ttctgtcatt cccgcataac ttgctgtatg atcaaagatt tttgcgacac ctgcaccaat
      721 atatccgcga tagcgcatag gcgtaccatt taaaatatct gccagcatat gtccttgacg
      781 attggccgca ctggaaagca tactttggat cggctgacca gtcactacac tagtcgtttc
      841 gataatatcg ccaatcgcat agatgtccgg cagattggtt tgtaactggt catcaacaat
      901 gatctgacct gtatctgata attgtatgcc ggttgctttc accacttcat tcttcggaga
      961 aacaccaaca gcaaaaatta gcatatcagc agaaaggacg gagccatctg ataatactat
     1021 ttctcgacct ttatcacgga tctcagtaac tcttgtttct aaatgaacgg ctaatccttc
     1081 tttcagcaat ttgtcataaa ctaaattagc aatttctgga tcataaggga aagcgacttg
     1141 tggcaattga tcgattaaag tcacgtctaa gccgcgatgc ttgaaattct cagctaattc
     1201 cagacccata acacctgcac caagaatgac gacatgttgt ggattttttt cttcaaggaa
     1261 cgatttgatt cgatccgcat ccgtcacact acggaggaca aagccgtttt ctgcttcaaa
     1321 aactccggga atatccggat aatctggtct agcaccagaa gagatgatca acttatcgta
     1381 atctgttttt atttcttcat ttgtggatag gtctttaact gttaatcgct tagtagaagg
     1441 atcgattgct gttacttcgt gtttagtaaa gacatcgatg ttgttttttg ttttcaatat
     1501 ttctggtgta cgttcgatca gcgagtcacg gtcagtgatc acacctccta aataataagg
     1561 caaagcacaa cttgcataag aaatattttc tccgcgttca tagataatga tttcgtgcgc
     1621 ttcattcaat cgacggaaac gagtggcaaa tgaaggaccg ccagctacac cgccaatgac
     1681 aacaactttc ataaaaacat ctccttttct acgttgatcg tactatgtta tggtaaaaaa
     1741 cagcaaataa ttgtgccgga aaattattgt ttattttgaa tagacagaaa atatagagaa
     1801 ataatttgtg gataaaacct aaaaattttt ttctatttta aggatagata atactttgaa
     1861 ggggataacc ctgtatattt tttaaaagct gaagaaaaat gatggatcga atgaaatcct
     1921 aatatatctg caatttctgt ataggttaat tcattttcta gcacgagacg ttttgctttt
     1981 tccattttta gttctttaaa atagctaata acactttttc cattcacttt tctaaaatgt
     2041 ttttttaagc tggctggact gataaaaaaa atttgggcta attgttcaag caaaaaattt
     2101 tctgaaagat gttcttctaa atatcgtatg attggatcaa ctaattcatc tgaataaaga
     2161 atatgctttt ttttaggaat gactttctca tttgaaggta ttgtcaataa agcaagttcc
     2221 tgttgataat gccttggtaa ggaagacaat tgaatttttt gcagacaata agtaactaat
     2281 aaggaaagca aatgaatgct catctgttgg catagtatga aaaaagaatt gatttcctga
     2341 tcataggaaa tagctggctt actatgtttt agataatcaa ttttgaactg ttcatttttc
     2401 agaaaagtaa tgatagatgt tatttcttga tctatagaaa aagattgtcc cgtaatatgt
     2461 gctaataatt gctcatcaaa ataaaactgg ataatgataa aagtttgtgt ttccttttga
     2521 gtagaaaaga tttggacttc ttgctgttta tcaatgaata aaagggaagg attatctagt
     2581 tctatagatt tatgtgcaaa ttcagcatat aaattagcgc tttcatttat tagtagtcga
     2641 aaatagggag cagcagataa atgaagatgg gtatctatgg aataggttag tgtttttttg
     2701 acaagattgg aggattggtt gtttgttaac aaaagaacca ctttcctttc tctttttata
     2761 taaaaagctt tctatttata attaatgact attataacaa aaatgaaaag ccaaaacgac
     2821 aagtaaaata tttttttata taaatatttg tgttactaat tccgttaata tacataagtg
     2881 aaagcgtttt atataagaac ttgaaaagaa agaagggata gacatgaaaa aaagatacaa
     2941 attggtgata gctttgctaa acattaccct attattcagc ttaagtgcat gtggaaaaac
     3001 taacgaagaa gtaacagcaa acatcgcaga tccaatgata aaaaaagcca ctacattaga
     3061 agtatatgtc gcaaacggca aactgacaaa ctatgttgaa aatgctgcaa aaatctataa
     3121 ccgcaaaact ggagcaaata ttgacttaaa aattgtgaat gtagcttcgg gaacagcaac
     3181 agtacaaatg atcaccccaa aattggtaac acacgaagat atgccggata ttatctccat
     3241 ggcagacact tcagcagcag gtgtgctgga aaaatttcaa gatgcttttt atagtgctga
     3301 tgaatatgga ttttatgaca aatatggcga tcagttttat gatcaaaaat tgaatatttt
     3361 gcgtcaacaa acgagtaatg aaacgattat tccttgggct agtgatttta ccccagcact
     3421 ttgttattat caaccaaaaa aatttgctga aataggtgta gattttgaag aaatcagttc
     3481 ttgggaagaa tatatccagg tggcaaaagc attaaaagaa aaaactggta gttttggaat
     3541 tgccttgcca gaatctgggg accaagaatt gtttatcaac atgctagccc agcagaatca
     3601 atctttgctt gatgaagaag gaaatatccg tctgaatact aaagaagcga aacatgctgc
     3661 aaagttaatc aaagaaatga tcgactcaga tattgtacat ttctacggtg cacaagatgc
     3721 tgagaaagca tttcaagaat ctgcaatgtt tgttgctggc ggctggtatg ctacgaatat
     3781 gtctttgaat tttcctgatg catctgacaa atggcgcatg gctccactga ttcctttttc
     3841 aaaagaaaat ccaggaaaag gtgcagtatc aggaggatca agtttttatg tccctaaaga
     3901 tgccaaaaat ccacaagtgg cacaacaatt tcttacattt atgctgacag atgaagaatg
     3961 cttagccaac gcactggagc tgggcgtggc tacatctaac tcattggcct atcaaacaga
     4021 agcagctgag aaaaagtttg actactaagg aggacaaaaa tactatgaac tattgaataa
     4081 aatcaatgaa aatacatcaa atactgtttt accagccact tattcagatg ctacgtctta
     4141 tttgactacc gctgcatata actattggca aacaggtgat tttgaaaatt catatctaaa
     4201 agaagcagaa aattttgctc ataagtacgg cgtaaaatta actggtgatt aaaaaatatt
     4261 aggaggactt aacgatgatg caatcttcgt taccaagcaa gacaataaag aacagcaaga
     4321 aaaaagcaac agatacgtct cataaaaaaa gtgcttatgg attggtcaca ccttttgtta
     4381 ttttctttat tgtgacgatt ttgattccca tcattatctc gtttgctttt agttttatga
     4441 aaattggcta ctcatttgaa tttgttggat ttgacaatta tctagctatc ttccgagatc
     4501 cattgtttat taaatcttac acgaatgttt tgattatcat ggcaggatca attccaataa
     4561 cgatgctatt agctatttac tttgctgttt tattgaattc agcacagtta aaaggaaaag
     4621 gattttttag aactgtctat tatattccaa ccattacatc aacggtggct gtagcttccg
     4681 tattcatgac ttttttcaat ccgactgggt tattcaatgg atttttgaat gcgctaggaa
     4741 ttaacggtgt acagtggtta acaaatcctt tttggatacg aatatcaatg atcatcacga
     4801 tgatttggct tagtgtagga tataacacta ttttattttt agctggtctt caagggattt
     4861 ctaaagagat atacgaaagt gctgaaatag atggagcatc gaaatttcgg caattctttt
     4921 tattaacgat ccccatgcta aagccaatca ttttaatggc cacagtttta gccactatca
     4981 atggcttggg taatttcaat atcccaaata tattctttgg gacttccaat ggaccagaaa
     5041 attcagcttt ggtcgtagga gtcaatttat ataaaacaag ttttgaaatg gttgattttg
     5101 gaaaagcctc tgctattgcc tggacgatgg tgtttgtatc tgttatatta tcggctattc
     5161 aatttaaatt cggaggtgat aaaaatgact aaacaaaaga tacaaagcag aatttttacg
     5221 tacgtcatta ttgtgtttgg tgccttaata tttttgatgc catatttata tatgattgta
     5281 gcttcaacgc aaagtaatca agaaatttta agtggcggaa tcaatttcaa gtttggggat
     5341 atgctattaa aaaactggca agccatcatg gcgagatata attatccctt gattatttgg
     5401 aattcgattg ttattgctgt aatcagtaca gttttagcta cagccagttc tacaatggcg
     5461 ggttatgcat tagcaaagta tcgttttatg gggaataaac tgttgttcaa tacagttatg
     5521 ctatctagga tggtgccttt ttttaccaca ttgattcctt tattttacat tatgagtcgt
     5581 ttaggattat cgaatactta tgcaggtgtg atcatccctt cgattgccag tacaacttca
     5641 gtgtttatga tgcgacaata tagcagtcaa tttcctacag cactgatgga ggctgccaga
     5701 atcgatggtg ccaatgagtg ggtgattttt acgaaaatcg ctgtaccgat tttatcacct
     5761 acgattatta ctagtggttt acttatcttt atgggaagtt ggaatcaata tctatttcca
     5821 ttagtcatgc tttcagataa agataaatat acggttccct taattatcaa aaatatgacg
     5881 gtttctagta caggtgagcc aattaattat ggcgcaatta tgctaatttt agcaacttca
     5941 gtgatcccaa tggtcttgat ttatgtgtgg gcgcagtcta aatttaagga gaatgacatt
     6001 ggagctgcaa ataagtaaaa aaaataacga atgttaacca tctagttcgt tctaggagca
     6061 actccacatt cataaaaatt aaaagaatgg cgggatatcc atgaatgaac agacgaagga
     6121 atggtcaaga aaaattttga atgtagcttc tgaaaagata gatgcggaat tagacagaat
     6181 ggggaataaa attccatata tttcttacaa cggaaaatat acagacgtaa taaaggacag
     6241 aggaatcgac tggtggacca atggtttttg gagtggtatt ttatggcagc tctttcacaa
     6301 gacaaaacga gaaaagttca aagaggcagc aatacaacaa gaacaattat tagaatctgc
     6361 cctgtaccat ttttcagacg ttcatcatga tgtaggtttt ttattcttac catcggctat
     6421 tgcacattat caacagtctc aaaacaaaca tagtcgacaa ctagggttac atgcagctac
     6481 ggtacttgct ggacgctata atatttcagg gaactattta gtagcatgga atgaccataa
     6541 acctggttgg atgattattg atagtatgat gaatttacca ttactgtatt gggcttcgag
     6601 ggaaactggt gatccgaggt tctctaaaat tgctcggcag catgcagata caacagctaa
     6661 gtatctagtg aggaaagatg gatcaaccgg gcatatcgca gaatttgatg cagaatcagg
     6721 gaaatatatt cggttacttg gcgggcaagg atacaatgag aattcttgtt ggagcagagg
     6781 aaatgcatgg gctttatatg gattcgcgtt aagttatcgt tacacaaaaa atccgtacta
     6841 tctagctata gcagaaaaga tagcagtaaa ttttattcag aaaatcgaaa aaactggtta
     6901 tattcctctc attgattttg ctgtaaaaga ttcgaatatt gataccagtg ctggagtatg
     6961 cgctgcatgc ggtcttctgg aattagcagg atatgtttcc tctgtacgat caaaaatgta
     7021 tcatgaagct gctatcaata tactccaaaa gacagaaaag cactatgcaa attggaatat
     7081 ggatgaagat gggatcattg gaggcggagc agaagcatac catagagaac tgacttataa
     7141 tgtgccactg atctattctg attatttttt tattgaaggc ttacttcgat tagaacagtc
     7201 ggaattagct atttggtagt aaaaagagga gaaaatacaa tgaaacatcc agtaccacta
     7261 ttaaggacca gacaggatta ctttgactta tttaaaaaga taaatcaacc actccagccg
     7321 ttttatcgag caagtggcgc acaaattgat tatatacatc aaggggtagg atatggaaat
     7381 aaaattgcag gaatggaagg attctctcgg gttttatggg gagcggggcc cgcaattgac
     7441 caattagatg aacgatggtt gaaattaatt ctatcaggaa taaaagctgg aacagatcct
     7501 tcccatcccg attattgggg agaaatacat gaaagagacc agcgtatggt agaaatgccc
     7561 gcaatgttat tagcgttata tcatcatgaa caattattat ggaaaaaatt agatacagga
     7621 gaaaaaagcc agattattca ttggttctcg caaatttttg aatatgaatg tgcagatggt
     7681 aattggcaat tttttaagat aattgtcgga acaattatcc aaaaatttgg ttatccagtt
     7741 aaagaatcag cactgaaaga agcatttgat aatatagaaa attgttatct tcaaaatggc
     7801 tggtatcgag acagttcaag aggtcgccaa gattattata atccttttgc atttcattat
     7861 tatggtttga tttatagcgt actagaacca gaaaagccca ttagccagat atataagaac
     7921 cgagctattg atttttctag agactatatt catttttttg cggaagatgg tgcaaatgtg
     7981 ccttttggtc gaagcatgat ttatcgtttt gcagtttcaa gcttttggtc agctatgttg
     8041 tgggccgatc ttttgccttt taaattaggt gaaatcaagg ggttagtcaa tcggaatata
     8101 agatggtgga tgcaaaaaga aatatttgat gaaaaaggta tcctgaatat tggttatacc
     8161 taccctcaat tgacattgac tgaaccctat aatagtacgt tatctccatt atggctgaat
     8221 aagatttttt tattgttggc gctacctgaa gaccatgagt attggacggc taaagaaatg
     8281 cctatgccta ttcgtgaagg aactaaactt ctttcagaag ccaatttgct catacaacat
     8341 gataatggac atacattttt tttgaatgcg ggacaattag ggccaaattt ccatacattg
     8401 accaatgaaa aatatttaaa atttgcttat tctagtcaat ttggtttttc gataccgcga
     8461 gcgaatcaat tgaaagcaga aatggcaatg gattcaatgc tagggattca gcgagcggac
     8521 acatatatca cgaccagttt taagaatgag agcatcaaaa cctatggaca gttcatcgtt
     8581 cgaaataatg tacgagacat tgtatgtaag aaagaggtac ttgcctctac atgggaacct
     8641 actttacagg caaaaatcag aacttggctt attccttttg aaggctggca gattcgcgta
     8701 cacaaagtag aattagatac agaatatgtt ctatatgaga cagggtttgc tattgaatgt
     8761 tctcctgaga aagaaggaat aatcatagaa gaagacaagg aaaactatag ggtgagtgaa
     8821 gctggctttt caggtattat ctgtttaagt gacgatacta ttaaacgaaa atccacagct
     8881 attatgggtt tgcccaatac aaacttgatg acatgggaaa acacttttat tcctggatta
     8941 gagggtacat ttggaaaagg aacccattgg ctcggaacag gagtggttgc tcatcaagat
     9001 cgggattata gtttagaagt atggaacaat cgtcccaaaa ttgattttga tgggacaacc
     9061 gggctaacta tccaaaataa aaacaataaa aaaagactaa aattatgcta aaggctggtg
     9121 gggaaatgct tgataaagtg atgtttgaag agataataca tccagaaaaa tggataaatg
     9181 aatgttataa agaattaatg gaaaaagaat ggaataatga gatcgagcgt tgtcgcagac
     9241 acacggagag gttaatcaaa cgtatcagta acagtcagta tcaattccca agcgagtgtc
     9301 ctataaaagg acgttatcca ttaagagaca atattggttg gacagaagga ttttggacag
     9361 gaattaattg gttgttattt gagaagacgg gtgatccttt atttcaacaa acagctgaaa
     9421 aagcagatat agattttatc cacaggattg actgtcaaat tatcactaat catcacgatc
     9481 ttggtttttt gtattcttta tcaaccgtag cagattataa aataacggga aatcgaaaaa
     9541 tgaaggaagc ttcaattgat gctgcagaaa cattacttca gcgtttttgg gagaaggcag
     9601 gagttataca agcttgggga gatcctagtg atccttcaca acaaggtaga atgatcatgg
     9661 attgttgcat gaatcttcca ttattgtatt gggcgagtga agaaactgga aatcaaaaat
     9721 ataaaaaagc tgctcaggct catattcaaa atgcagcaga ttatttagta agagaagatg
     9781 gttcaactta tcatacattt tatatggatg tcaaagatgg aaaaccaaga tatggaagta
     9841 cgcatcaagg ttatagtgat aattcctgtt gggctagagg ccaagcatgg gggatttacg
     9901 gttttgcatt aagttatcgt tatttgaaag aacaaaaata ttttgattta agcaaaatat
     9961 tagccaatta ttttatgaat cggttgccag aagataccat ttgttattgg gatttgatct
    10021 ttgatgattc tagcagagaa gaaaaagata catctagtat ggtgatcatg gtttgtggat
    10081 tattagaatt aatagaatca ggtctattag acaaaacaga aaagaactta taccagtctg
    10141 ctgtttggaa aattatgcga caattgaaag aaagatatgc tacaacggaa gaagaagatg
    10201 gctttttaac acagtctgtt tataataagc ccaaaaataa tggggtaaat acaccttgta
    10261 tttggggtga ttactattat ttagaagctt tagtgagaat gactagtaat tggtcttctt
    10321 attggtgaaa aaggtaaata tggaattttt gtttgataaa ataaaaaaag aaaaaccaaa
    10381 acagcatgaa tggtttctgt tttggttttt tcgttaattt tcatattctt ctaattccaa
    10441 gcttagttct tcccattccg tcaattttgt ttcttgatct tgtctagcag cttctaaatc
    10501 ttggttcagc tcattgagtt ttacgtgatc ttccaggttt tctggcgcga ccatttcttg
    10561 ttccaactga tcgatccatt catcgatccg cgccatttct tcttcaacct gagtaatttt
    10621 tcgttgaagg ctacggataa gcttttgctg ttctttgctt tggtaataat cagatttgtt
    10681 cgtgggagca ggagcagatt ctggctcatc ttgtgccaac aattcggcga tttcttcttc
    10741 ttcctgcttt ttctctaaat aataatcata gtctcctaga tagactttgc tgccattttc
    10801 agagagttcg acgacttttg tcgcgatccg attgatgaaa tatcggtcat gggaaacaaa
    10861 aagcagtgtg ccgtcataat cgatcaatgc attttctaat acttctttgt tgtcaatatc
    10921 taaatggttt gttggttcat ccaaaatcaa aaaattttct ttgttcatcg ctaacttggc
    10981 taaagcaacc cgtgcttttt caccgccact tagcagaggg attggttttt ctacatcttc
    11041 gccagaaaat aagaaagcac ctaaagcact tcggatctct ttttctggag tggtaggatg
    11101 ctcatcccac aattctgcta ggatcgtctt gtttgtatgc agatcagctt gaccttgatc
    11161 gtaatagccg atggtgacat ttgtccctag cgtttctttt ccacggataa aagggatctg
    11221 gtgaatgatt gatttcagca acgtcgattt cccgatacca ttcggaccta ctagagcaat
    11281 cgcttcttga cgacggatat ctagattgat tggttcagat aaggtgacat tatcatatcc
    11341 gatagcggca tcttcgattt gcaagactac atttccagaa ggcttttcta tactgaatag
    11401 aaagtttgct tgtttttcat ccccttgagg acgatccagc cggtccattt tttctaatgt
    11461 ctttctgcgg ctttgcgctc gttttgtggt agaggcgcga acaaggttcc gagcaacgaa
    11521 gtcttctaat ttatggattt ctgtttgctg cttttcataa gccttccatt cacttgctaa
    11581 ctgttctgct ttcaaatcta gatatttcga atagttacct ttgtaatggc gcatttttct
    11641 tctgcttaat tcatagactt cattgactac tttatctaaa aaataacggt catgggaaac
    11701 aatcaataat gcaccagtat agctttgcag ataattctca agccaggaaa gtgtctcgat
    11761 atctaaatgg ttcgttggtt catctagaat caaaatgtca gggtgttgca aaagcatgcg
    11821 agctaaagct aaacgtgtgc gctgtccgcc tgataaagta tcgattttct gatcataaaa
    11881 tgaagaatcg aatttaaacc catgcaatac ggaacgaatt tcattttcat aaccatagcc
    11941 gtttcgatct gaaaaatcgt gttgcagccg gtcgtattct tttaaataag aactataagc
    12001 aggtgtgtct ggcgtacctt cgctaatgaa aatctctagc tcgcgcatcc gctcttccat
    12061 ttgtctaact tgttcaaaag ctttcagcat ctcgttccaa actgtttcat tggaatcaag
    12121 tcctgtgtcc tgagcgaggt atccgagagt cgctgtttta tttttagcga tctttccttc
    12181 atcaggtgct tcaattccag caatgatctt taataaagtc gattttccag cgccatttct
    12241 gccaactaaa gcgacacgag cgcctgtact gatttctaaa tgtatgtttt caaataagac
    12301 ttctgcacca aaatggcgag caatctgatt ggcttgtaat aatatcatgg aatcctcttt
    12361 tctactttgt gaattctcct caaataaaaa actatttttc aattagtagt ctaccataaa
    12421 ttccattata ctgcgagatt tgcagaaatg ataaataaat gaataaagaa taaaatttca
    12481 caatatctga gcaatggaat tgattttttc ctataaatac tgctatgatg gagaaatcaa
    12541 agatgtaatt agacaatacg tggaggtcat ttcgtgaaag atcatattat tccaaaggcg
    12601 acagctaaaa gactgccttt gtattatcgc tatttaagga ttttaaataa tgctggaaag
    12661 aaaaaagtgt cttcaactga attaagtgaa gccgttcaag tagatagtgc aacgatccgt
    12721 cgagattttt cttattttgg cgaattagga aaacgtgggt atggttatga tgtagaagat
    12781 cttatgcact tttttgcaaa gacattaaac gacgatcaat tgacaaatgt tgcgctgatc
    12841 ggtgtaggga acttgggaag tgctttacta aaatacaaat tccatcaaag taatagcata
    12901 cgtatcagtt gtgcttttga tgtgaatgag gaaatcgttg ggcgcatcgt agacggtatc
    12961 cccgtttatc caatggaaga tatgtcagaa caaatcaaga tccaacaaat cgaagtagca
    13021 attttaacga tacccgctga aagagcacaa gaagtagtca atcagttaac ggaagcagga
    13081 atcaaaggaa tcttgaactt taccgctgct cgattgaatg ctccgtcaga tgtattgatc
    13141 caaaatgttg atttgacgaa tgaattgcaa accttgatct atttcttgca ttctgacgca
    13201 aatgcgcatt ccgaaaatac agcagaataa aaataaaaag cgattgaaca aatgaaggca
    13261 aaactttcac ttgttcaatc gcttttttct gtttggacaa cgattatttt gttcggaaga
    13321 tttcttcata agaaagggaa tcatcttctg ctgtcagttc atctagtaga taacggattt
    13381 ttttaaagtc gatgaaccgt gcttgccgaa agacttcttt tccttgatga aaaatcagga
    13441 caactggcgc agtcagcact tcaaatttac tagctacttc tggtgtttca aatgcatcca
    13501 aatgaagtgc cggaatatca taatgtgtga gaagttcctc tagtcgtgga cgaacagcgt
    13561 gacaaacact gcaattaggc atcgaaagat aaatgacggc aacggatgac tgttgcagta
    13621 tgtcttgaac ttcagtcaag ttagtggttt gtttcatttt tattctccct tttatgatag
    13681 aagtgatatg tatgagacct taatttaatg ggaataacag aaaaagtcaa ttgaagtgct
    13741 ttggtaaaaa ataagagttg tgtaaaaatt tacagagcaa cttgtttttt ttgaataaaa
    13801 gcgtatattt gcaaagcgaa tgaactagaa ataaaaagga ggaaactcat gttttctttt
    13861 attccaaatt cggttttgca gatgagtacg attttcttat cgatcgttat tgaagcatta
    13921 cctttcgtat tattaggctg tcttatctca ggcgccttac aagtattttt gaccccagaa
    13981 cgtgtgaaga gatggcttcc tgaaaatcgt atcttctcta tttttactgg ttcgatctta
    14041 ggatttttct ttccttcatg tgaatgcggc atcgtaccga tcgttcatca atttgttaaa
    14101 aaaggcgtac cagttcacac agcgtttgct tttatgctga ctgctccgat catcaatcca
    14161 atcgtgattt tttccacttt tatcgcattt ggcaattctt ggcagatggc aagctggcgg
    14221 gtgataggca gcttttttgt tgcccttgtg attggtgtct ggcttgccta ttttcaaaaa
    14281 gacgctgtat tgaaagaaaa aatcacgtct aaagtagcag aaaatcacca tcatcatgtc
    14341 catgaagaag aacaaagtga gaagaatgaa ccaaaaaatc agctgatcca tcaaactggg
    14401 catgtgttga cacattcaat cgacgaattt tttgatacag gtcgttattt gatcattggc
    14461 ggattaatcg cagctgcgat gcagacttat ttaccaacta gaattatgct aacgctcggc
    14521 tcaacaaaga ttttagccat tatcatcatg ctgttattgg cctttacgat gtctctttgc
    14581 tcagaagcag atgcgtttat aggatcttct ttgttaagtc tgttcggcac agcacctgta
    14641 gctgcctttt tggtctttgg cccaatggtc gatatcaaaa atctactgat gatgaaacga
    14701 tattttacag gacgctttat tttatcgctg atcggactga ttgcccttag cgttattgga
    14761 ttcacattat ttatttagga gggaaaatat gctgcggttt ttgattttat ctgggtattt
    14821 cgaattgatg atgtacttgc aagtttctgg caaactcgat caatatatca atgtccatta
    14881 tcgctactta gctattcttt caatgatttt atctgctctt ttggctttag ttcagctgta
    14941 tttgtgggtc aaaaacggaa aagcagaaga aaaggcacac gagcacgatc atgaccatgg
    15001 gttgtcaaaa ccttatcaac gtggaatcgc ttatgtactg ctagctttgc cagtaatcgt
    15061 ggggacgttg tttccaacag ttagtctcga cactacgatc gtagaagcca aaggatttaa
    15121 tttcccagta agtaaagaat cagtagggga tccggagatg cagacacaat acttaaagcc
    15181 ggataccagt atttatttca acaaatcaga ctacttagac caaatgaatg agttgaaaga
    15241 aaagtatggg gataagcaaa ctatcaagat cacagatgaa aactatttag aagttatgga
    15301 gctaatttat aattacccta gcgaattcat tggcaaaaaa atcagctatg aaggatttgt
    15361 ttaccaaaca ccgaacgata gcaatacaga tatcttcttg ttccgattcg gcattatcca
    15421 ttgtgtggca gattcaggag tctttggttt gttgacgcat atgccggaag gagtaacggt
    15481 taaaaatgat gaatggtata aaatagaagg aaccatccaa tcagactatt atcaaccatt
    15541 caagcgtgaa atcccatctg tagttgttac aaacgctgag aaagtctctg ctccgaaaaa
    15601 tcagtatgtg tatcgttctt tttaaaagaa caaaataaat aaacatagct cagacagctg
    15661 acacgcaagg acgtcggttg tctggctttt tttgcatgat gtattcattt gtttgatatt
    15721 tattttagaa gcagcatcct gaaagtattt gatatcggtt tcaaaggtgt tggaaaagat
    15781 tgtgaagaga attacaattt attcctaaca tgtatgttga aaagttgtat gaaatgattt
    15841 tgatagtaaa atatcgagtg gaggtacttt ttatggtaac gcgtatattt attttggatg
    15901 atagtttggt ttttgaaaaa atgctagtag aaattttgga agaatccaga attttattaa
    15961 gtccttggaa atttgagatc gttcagggat tgaacgttat acaatttgtt gaattcatta
    16021 aaaataatga aatctattcc tcagatattt ttttcctaga catcgatttg aacgcctatt
    16081 atagcggtat cgaactaggc gaaatgctta gagaaaagaa tgaaaagtgt aaaattgttt
    16141 atctaacgtc tcatccgaat aaagcaataa ctgttattaa tcgaaagatt acgcctagtg
    16201 gttatttgat aaaagaattc acttatgaaa aaaataaaaa aagcatccaa agttacttat
    16261 tgaaagaaaa agaaaactat cctgttcaac atgctacgaa aaaaaggtgg acaactgtcc
    16321 gttatggtaa tagcgatcac tatatccatt acgatgaaat cctttatcta atgagcatgc
    16381 caggttataa aaataaaata ggcctagtat tgaaagatga agagattctc gtaaacggca
    16441 caattaaaaa atataaaaaa tcactcgaat atgagtattt atgtaaagat ttaaaagctt
    16501 atatcattaa tactgaaaac attacatcta ttagcagagt aaacgaaata gtaacatttg
    16561 caaatggaga acaactagag atgggaaaga gaagtattga taaactaaag aattttatgc
    16621 aagaaaagat agaaaatgaa tcattttaaa gaaaacaaag actcatatct tacctgttag
    16681 aagagtgttt tcctgtagaa gagaacaacc caagtagcag cctagtcgtt tctatccttt
    16741 tttgttatgc actctttttg acatttactc caccaataac gaagggtaca ataaaaagta
    16801 ataacaaaca aaaggagtac agaaatgatt gaattcatca atgtaagtaa aagttacggg
    16861 aaaaaacaag cattaaaaga cttgtctctc tcgataagac aaggggaaat ttttggattt
    16921 ttaggacata atggtgccgg aaaatcaact acgattaaaa gtttagttag tattattgaa
    16981 ccatcatcgg gaacgatttt agtagatgga atgaaattga cggaaaatcg attgagtatc
    17041 aaacaaaaaa tcggttatgt gcctgattcc ccagatattt ttttacaatt gacggctgga
    17101 gaatattggg acttgatttc agctgcctat gaattagaca cacaaaaaaa agaaaagcgt
    17161 ttagcagaat tgacagcgct ttttgatatg tatagtcatc aaaatgaaac aatcgcaagt
    17221 ttttcacatg gaatgcgtca aaaaacgatt ttgatcggca cgttgctgcc agatccagat
    17281 atttgggtat tggatgaacc tttgcaagga cttgatcctc aagcagcatt tgatttgaaa
    17341 gaaatgatga aggctcatgc cgcaaaagga aaaacagtca ttttttctac acatgtattg
    17401 gataccgcac aacagttatg cgataaactg gctattttga agaaaggtga attgatttat
    17461 cagggggcag ttagtgaatt attgaatggc tcacctgatg aaacgctaga aaagatttat
    17521 ttgaaaatgg ctggaaggca agcgacagga gatggaccgt atgtataagc gacaattatt
    17581 agaattaatt cgagttaatc ttttatatgt taatccacag ctcacgaata aagcaagaga
    17641 gaaaggaaaa aagggaaagc gattaactat ctatcttttg tctcaaaatc ttttttcagg
    17701 gctgattttt ttactgattt acggatttgc tatgtttgct atgaactttg ttaaattgcc
    17761 aggtttcttt acttattacg tggcactttt tggtatgtta ggtttaactc aaagtatatc
    17821 agtcatttat aatatctttt ttgaaggaaa tgacctgcaa gtatttttgc ctctgccttt
    17881 tggacaagaa caggtttttt cagcaaagat tttggtagtt gctttgacca tcattccttt
    17941 tgtttttccg cttctagtca tgtttatttt aaccggatgg cgagctgatc tagggatttt
    18001 gcttactatc attatctcaa tccttttgtt ttctctattt ttgattattc ttttttgtct
    18061 ggctagtttg attgtttttg gtttggctcg aacagcagtg tttaaaaaac atagaaaaac
    18121 agtttctagt atcttgcttg gcagctcgat gctgatcgca tttgctggaa tcatgtggat
    18181 gaatcatcaa acggggacta ttgaaaatga gttaacagat cggcatccga tttcgatatt
    18241 attgccattc tatgatgctg ccagtcagcc actttctcaa caaggtttat tgggttgggg
    18301 aatactagct atcacggctc tgggattttt ccttgctgta aaacagctga tagtaccaaa
    18361 gcttacagag caactgactg attcaaacag tgagttcaaa ataaaaagaa cccataaaaa
    18421 gaaccaaaat gtgcatcagc ttttatttag ttacaatgct caattagtaa aagaacctaa
    18481 tttgattatg caagttcttt cttcttcctt gttaactcca atcattttta ttcttgcttt
    18541 tgccctagga ggcgaaataa aattaactga tttagataat aagttaatcg gtgttgtttt
    18601 cctggtaggg atcgcattgg ctttcttaac agttaatcag acttctttta tcagcaatct
    18661 tatttctttg gatcaagaaa attttctgtt tatccgttct ttgccaatct ctatgaacca
    18721 atatttaaaa gaaaagttca gattcggatg gatattgcag agttttttaa ctggtgggat
    18781 cgcattatta agcggactgt cttttcagct gcctttgttt tttatcccta gcttgctatt
    18841 tggttcttta ttcggctgct acttattgtc actacgttat ttcgctagag attatcggtt
    18901 gctgctgctg aactggacca atattaacca attatttaac agaggttcag gaagtttggg
    18961 tttagtcgca actatgatgg gttcattgat catcagtatt attcttatcg tcttatacgg
    19021 aatgttggct ttgttttatc cattttggtt agtgaatatt cctgtgatcg gtttagttct
    19081 aatactttcc gctctatgga tcgtttatta tcaacgaacg ttttggaaaa aatttgaata
    19141 aagaagaggg gccgtgacaa aaatcatgtc gcagcccctt ttttacagat agagggtgtt
    19201 caaaagtcag tttctcagta ttaagtcaat tttatgtaaa aatgtgagaa gctattttta
    19261 caaaactttt tttaatactc ataattactt cttattgctc ctgcggttac ttgtcgcaat
    19321 gtttgtgaga tattttgtca tcgctcaagg ctgaacgtct ctttcacaac cttttctttg
    19381 ttacacttcg tttatttaaa aagccgtttt tttcgtttca tcatgattgc gtcacgcttt
    19441 actttgaaat tacggatgtt atggttaaag acttctgata atacaagacc taaagcgatt
    19501 gctccagcaa tcataattgc ttggaccgca taatgagccg cttctcgata ggattcagta
    19561 acgagatttc gaaccgcttg ataagctaaa ccaccaggga ctagaggaac catcccggga
    19621 atattgaaaa tcgttacggg cattttcaaa cgtttagaga aaagatcgct gatgaatgct
    19681 actcctaaag aaccaagcaa tgaaccaaaa gcagcagatc cgcctatttg aacacagatc
    19741 cagtagatca tccagccaaa agcaccagaa agaccacaac aaatcaatga acgcctaggt
    19801 acatttgtaa taacggcaaa tgccgcagtc gctaaaaaac taaaagaaaa ctgaataaat
    19861 atcgtcccca tacttgtgtt cctttcctaa taaaaaagct ggaaaataaa agcaatcgca
    19921 aaaccgatca tcgtagcagt catcatcgct tctgttccgc gagaaactcc tgagacataa
    19981 tgtccagcta gcaaatcacg gatagcgttc gtaatttgaa ctccaggaac taaaggcatc
    20041 acacatccga taatgattag atcctgattg cttcctaagc caaaacgtac gctgtagata
    20101 gcggctatgc caatcaataa agaagataaa aattccgata aaaatttgat tctaaaaaat
    20161 ttcaaactat atagataagc tgcgtatccc aatcctccaa tgactaaagt cacaggtaaa
    20221 tcggtcatct ttccgccaaa taacaccata attgttccac tgatcactgc cgcgctaacg
    20281 atttgcaacc agacaggaaa gaaagaacgg tctcggtcga ttgcttgcag ttcgcagtaa
    20341 agttcttcta atgttagctc tgaagcaaca tatttacgtg aaagatcatt tacttttact
    20401 actttttcta aattaatcga acggttggta atttgttcca ttcggatggt cgaggtacga
    20461 tcaagaccaa taaagagacc ggtttgagtg acatagctga ccaaacggaa atttccagaa
    20521 gctaaagcaa ttcgactcat cgtatcttct acccgataca tctccgcatt gctttccatc
    20581 atgattttcc cagcgagtaa acaagtgtcc agcaacagat ctaattcttt tcccgtcata
    20641 gcatcttctc caataattga ttgattagtt ttattttact acaaaacata ccagaagcgt
    20701 aaagaataaa aaaacttgag gaaggaataa aagaggcgta tactgttttt gtattgcggg
    20761 tgtagtttag tggtaaaatc ccagcttccc aagctgatgt cgcgggttcg attcccgtca
    20821 cccgcttaaa ataggttgtg aaaaagctgc tctgcaacaa gtaataagaa cagccaagca
    20881 aaaacaactt cttatgtttt taacttggat ggacttatta ccgcagatgc aagcttttgc
    20941 gcaccgttta ccaagcggtt gtgaaatcag tgtattgtct tgagtatgaa gaaaattttt
    21001 gaataataat aaatacctta cagatagagt ggtgactgat tagagacgag gaatccgtct
    21061 cttttttgta tttttaagcc atcttaaaac aggttttatt ttttgctgtt tctatttctt
    21121 tctagtattt atttataggg gaacatatgt taagatttag ggagctgtta gtaaaggagt
    21181 aaagaaatga gcaaaaaaac aaccaaggcg atcgtgggga tcgtcgctgc tgtgattgca
    21241 ggggcattcg gtgtttccac cacacaaaaa gattcagata tctttcagca gattacaact
    21301 atttttgatt ccacaccaca agcttctcaa gtagcggatg tgccagaata tgatggaaaa
    21361 catcaagaaa tagaaatcaa tgggaatcag ccggaattta caaaagagga tcttagcctt
    21421 tccaaaggaa catgggaaag ctattcaaat attgataaat tgaacagagt aggacaagcc
    21481 aatgctatgc ttggaaaaga tttgttccct actgaaaaga gagagccttt gtatattgac
    21541 ccgacaggtt ggaaacaaaa gaaattaagt gacgggcaat ggttatataa tcgcagccat
    21601 ttaattggtt tccagctgac tggtcaaaat aacaacatta aaaacttgat gacagggaca
    21661 cgttcgttga atgctccata catgcttgct cacgaaaatg atattgcggc atatatcaaa
    21721 gaaacaaatc atcatgtacg ctaccgtgtc actcctcatt ttgaggggaa tgaactggta
    21781 gccaggggag tgcaattgga agcagaaagc attgaagaca aaaaaattga attcaatgtc
    21841 tttatctaca atgttcaaga tggctacacg atcaattatg agacaggaca agcaacaaaa
    21901 acaaaaaatt agtagcagct attcgaattt tttatgaata ttatgaaaaa attgtgtaaa
    21961 aacatacatt caaagaaata tagcgtatac tttatgaaaa gagtactttt gataaagcta
    22021 cagaaaaaag gaaaaaaaca tacaaatagt gtttcttaat ttattcggaa gtttggaata
    22081 tttgtttgta gttttccctt tcttcgagta aaataaggtt gtagaaggta agctggaatt
    22141 accttgcggg aaagaggtgt agaatcatgg gttttacaga tgaaaccgta cgttttggtt
    22201 ttgacgatag tcgcaaaaaa gaagtgagcg aaacattagc gattgtttat cgggctttgg
    22261 aagaaaaagg ctacaatcca atcaatcaga ttgtcggcta cttgctttct ggagacccgg
    22321 cctacattcc tcgttatcgt gatgctcgaa atttgattcg ccgtcacgaa cgcgatgaga
    22381 tcatggaagt aattgtcaag gactacctct caaatcatgg agtgaattta tgagagtaat
    22441 gggcttggat gtcggttcta agacagtagg tgttgctatc agtgacccct ttggctggac
    22501 agctcaagga gtagagatca ttcggatcaa tgaagcagaa gaagagtttg gctttgatcg
    22561 gttgaaggaa ttagtagcac aatatgaagt agaccggttt gtagttggat tacctaaaaa
    22621 tatgaacaat tccatcggac caagggcaga agcttccatg tcatacggag aaaagatcaa
    22681 agaactattc tccttgccag ttgattatca agatgaacga ttaaccactg ttcaggcaga
    22741 acgtatgcta gtagagcagg caaatacttc aagagccaaa cgaaaaaaag tcatcgataa
    22801 attagcagct gtgatgatct tacaaaatta tttagacggc tcaggtaagc aattatttta
    22861 ataataaaag aaaagaggta atcttatgac agaacataac cattcccacg accatgaagg
    22921 acacgaacac atcacattgg tggatgacca aggaaatgaa acattatatg aaatcctact
    22981 aacagttgac ggacaagaag aatttggacg taactatgtt cttttatatc ctgctggtgt
    23041 ttcagaagat gaagatgtag aattacaagc ttatgcatac atcgaaaacg aagatggaac
    23101 agaaggcgag ttagaacaaa tcgaaacaga cgcagaatgg gacatgattg aagaagtatt
    23161 caatacattc atggcggaag aagaagaata gactataaag ctaggagaaa ccttgatatg
    23221 atagggtttc tccttttttt attttttact gtatgccata ttgtatgcca tctagtcaat
    23281 atttaagtgt tgtactaatt tttcaacagt cttttctttc gtttcttttg ccaagtgacc
    23341 gtaacaatcg atcgtctgac taatttttgt atgccgtaat ctagtttgaa cttctaccat
    23401 agtagctcca gcgtccaaga gcattgaagc atgagtgtgt cttgctacat gcacaccgag
    23461 aaatggaacc atagcttttt cacaaatagt aatcaatctt gcataaatag cgtggtgtgt
    23521 gattagttct ttatagatac cacaaaaaat catagtcgga tcagttacac caagtgtcag
    23581 catatatttc cgctgattaa tctgccattt ttttaatata gaaattgttc tttcatctag
    23641 tggaagaacc ccagcactac aatcagtttt aggatcactg attttatagc catttttgga
    23701 ttggcttagt gttttattga tggtgaccgt attttcttga aaatcaatgt ctgaccaatt
    23761 caatgcaagg gcttcactga tcctaacccc gctataaaag agaaagcgta gtaacgtttt
    23821 atcaaagtcg tttcgataag tttcaggttt gctgtttagg taatcaaaca aggtgatcag
    23881 ttgttcttta ctgtaaactt tgaccttttc tttatgctta gtggtggttc ttttaggcat
    23941 taatacgttg tctagtggat tagtctcaat aatcccaaga ttgatagcgt agctaaaaat
    24001 acgcttagaa gttgaatgaa gtaccttgta ttggtcagta gtttcagccc agctatttac
    24061 aatttgttgg gcttttttta ccgtgacttc attcacacga agatttccat attttggaag
    24121 aatccacttt ctaaacttga tttcagtttt catgaaggtg gcttctcgga cagtggtttt
    24181 atatgtttct agccacaagt cataaacttc tttaaacgtt tcgttgttcg cttttttaat
    24241 accgttttta tcgtagtcca cttgcaagcg tgacaaggca agctgggctt ctttttttgt
    24301 tttgtaacct cgtctacgaa ccttatcagg tttgccagtc attttgttca ttccaagata
    24361 ggcttcatag agccaaaatt tacctttttt agtttcatat tgcttgaatg ttgccataat
    24421 agttttccct ccatagtgta gcaagccgtg ggggcggttg gaggaggcag tttattatta
    24481 ttttgaatag tgtttattta gctcagtaac aagagctgtg aactctttta gaagatcatt
    24541 ttcataaaat tcttgatact cttttttctc atctgtattt ttttctgaat cattaattaa
    24601 tgagtcatat cgtaaaagcg tatttagaag agctgcgaga taagcaattg gggtactccc
    24661 gtcaggaaat tcaattcgag atcctgtttt aagatatttt attgcgtttg ttaaataaat
    24721 cttctgtgag acagtcaggt tatcttcttc gatgttattg atagcattag aaagattaga
    24781 atcaattata gagttatttt tttcaatagt ttttttcaat tgcttttcaa gcacatttcg
    24841 cttaatttcg ggcatcatat gcatatctct taatgtataa gatccgttta atagataggt
    24901 agtggataca tctaatacct ttgaaagttg tttaagcctg gtgttattgg gaagattata
    24961 gccattttcc caattactaa cggcaccttt actggccggt ggggataatt tacctccaag
    25021 ttgttccatt gagagtccct tttcaattct agccagtcta attctttgtc ctgtctccac
    25081 tgcttcgggg tatttttttc tagccacatt ttcaccacca ttcatatatt ttctctacta
    25141 tatcacgatt taaatatgtg tgaaacataa aagttcaaaa aagtatgtta aaattcttgc
    25201 aaatgaaaag gtatgatggt atattgagtt tataagaggt atagaaatgt attcaagagc
    25261 ctcttgcatt tttttaaaat ctttgtatag aaatgtatgc taacgagggg ggataaatat
    25321 ggaattaaaa attgacaaac tcacgcttga tgatccagct tttaaaatta ttgacgatcg
    25381 agttgttcaa caagtagagc aaactcaagg aacgttactt tcattattag aaaaaaagta
    25441 ccagtttcca aaatatatga acaaggcaca agctgctaag tatatgaacg tttcatacaa
    25501 tactatgatg cagaaatatg ttcctaacgg tttaaagttg attatagttg atggagtcgt
    25561 aagaatttca caagatgaat gtgatcgctt tatggaagaa taccaaaaat aaacgcaagc
    25621 cgtgggggca aagggattaa taggaggaaa taataatgga atcgacacaa aataaagcaa
    25681 tcgaaaaagt attatcaaca attcttacag aagacacagc ggcagaatta gcaaacttgg
    25741 aaggcaaagc gctagaagaa acgtttgaat gtttgtacga acaaatggac tatcaaaaat
    25801 tgctaccaca agaaccaacg gtaagcggag tattgcgtgg cttgaacgat ttagtacaag
    25861 cagagtttaa agaacgactt tctattgaag agtatcaaga aattttgtat cagcaagttg
    25921 agttgttagc gagcttgcta gggcttgaac tggaggacgt tgaatgaaaa ctgaaatttg
    25981 gaacggacac attataagat ttgtcgatat taatgatgaa tggtgggcag tggcgaaaga
    26041 tgttgcggaa gcgttaggtc ttaaacaggt tactagagcc attcactctt tgcctaaaga
    26101 tggggttact actagtaagg tcatcgattc attgggcaga acacaagatg taaacatcat
    26161 caacgaaaaa aaatatttac cgcatggcat tcaaaagccg taaaaaagaa gccgaggcat
    26221 ttcaagactg gattttcgac atcatcaaag aactacgcca gtccacagga cttgaaggct
    26281 ttcaagtatt ccgcatgcta gacaaagaac accaaaaaga agcaatgact aaattaagcc
    26341 acgctattac tgaaccaaaa ccagtagact acattaaagc gaatgtgata gccaataaag
    26401 ctgtatcaac tatttatggt cattccaaaa tggtaaaaaa gaaagatatg actcctgaaa
    26461 tgttggttga tcgtgaacct attttagatg agacagtaga attaatgact gttaaagaaa
    26521 aatatgggtt gcagttttca gtgagtgaga aaatttataa tcgttctgct gaattgcaga
    26581 caacttagga gcattaacat gatcgagaac aaagaaaaag ccttatccat aacagtcttg
    26641 gcggacagga taaaggcaac ttaattggga taaaacccga tattctttgt tccgattata
    26701 ccatattaaa aaatcggagg gaaccattat gaaacgaaac ttaattatag gaagcatttt
    26761 aatcggcgga attttcggct tattaccatt aattgcaaaa atagctatct ttgcaggatt
    26821 agcgttatat ctcttcactg cttatgacga gtacgactat caattaagaa ctggagggca
    26881 taagtagatg tacaaaacaa aactattaaa ccaactcgat agccttgaat tagaggaaat
    26941 taatcaaggg atcgctgaac tagaaaataa tatcgggaaa acctactttg gaaattcatt
    27001 caatgaaaaa ctaacagttt tgtatgtact caaaaaacat gcagaacaca aaataatttg
    27061 tcgagaaatc aacgagctaa aaaatcagat tttaaccgca tggttaaaca ttactgatat
    27121 gcaagaagca agggtgaaaa catttaatac atgggtaaag tatcagaatc aattaaaagg
    27181 tgctgaattt gttcgggatg ggttgaaata cgaacttgag cagttgaagc tcatggaggt
    27241 gtctgaatga tttataccag tattttgaaa aaggaggaga ggtttattgg caaataatta
    27301 tcaagcagca acggaattac aaaagtcggg atggtcaatt tatcccttat ctcctggaac
    27361 taaaattcca ataaaaggct ctagtggttg gaatgatgca actaacgatt ctaatcaaat
    27421 taatgaatgg tggacggaag atgcaactag aaatattggg ttaatgcttg aagatcatca
    27481 aatggtggta gttgacctag atcaacattc tgaaacacga aacggcgttg agaattataa
    27541 aaaacttgct tcaatgtatg atccatttcc ttctacttat acggaaatca caccaggtaa
    27601 aggaatccat ttcttcttta aaattcctga tggtgtaatt atccaacaag aaacaggtgc
    27661 atttgctgat atcttcgggc gtgatgataa aggtaaaagt ttaagcggga ttgatattat
    27721 cactaaagga gttccaatag cgccaactat gattatttca agaagtgttg ggagagcata
    27781 tgaacctatt gaaaacgtat ctctaaataa tattgcacca gctcccgatt ggctagtgaa
    27841 cctactgcta aaaaaaccgc aagaaataaa gactcaatac cgacccgtag caaaaacagg
    27901 gacagcaaaa aaattagata tgattgttca aggcgctggc gaaggtagta gaaacgatca
    27961 tttaactaag ttatgcggtt ggttactatg gcacggtgta gataatcaaa ctttagcaga
    28021 attgatttat acagcaaatg cgtacaatga accaccgcta aaagataaag aagtgaatca
    28081 aattattcga tcgatgatta aaaaagattt gagggggaaa gctcatggct cataagcata
    28141 aattagatag tattttagac tttcccgaag ccagcgagcg tgaagacaat atcattgaat
    28201 tgaaaacctg gatgtcacga ttaagatgca ataaagacga tcagattaaa agtaacagtg
    28261 ttgtgaatgc agaattgatt ttaaccaatg atagcaattt agctggaaca attgcttata
    28321 acgaatttag cggatacatt cacctattaa aagattctcc ttggatcaat cgtaccgctg
    28381 gagaatggga agacagtttt gaggatgcct taacggcata tattgaagaa aactacaatg
    28441 tagtttttga tgacaacaaa atacacaaag cagtagtaaa tgtggctaga aagaatgttt
    28501 tcaacccagt aaaagaacgt atcgaaaaag ttaaatggga tcaacagcct agactggaaa
    28561 cactgtttat tgacctttta ggggtagacg acaaccttta cacacgagaa gtaacaaaac
    28621 gttggatagt cggatcagtc gcacgtatct ataaaccagg aataaagttt gagatcgttc
    28681 cagttttgga tggaccgcaa gggattggta agagtaccgt tccagcatta ctttacacgg
    28741 atgatttttt caccgattca ttagattcgc taggtgaaaa gaaagatgat tatatgcagc
    28801 ttcaaggcaa tgtaattatt gaacttggag aattatcttc aatgaacaaa actaaaattg
    28861 aacagataaa aaacttcatc agtgccaaga tcgataaaat tagacctcct tatggtcgga
    28921 atgtcatagc ttgggcaagg caatgtgtat ttattggaac gtcgaatgat gggcagtatt
    28981 tgaaagatga tacaggaaac cgccgttttt atcctttgcc ttgtaagaat aagccaaaga
    29041 tgaatccgtt taaaacaaag gatgaatatt ttcttcaagt gttagctgaa gcaaaggtgc
    29101 tttttgataa aggacaacga atctatttta gtcccgatga agataaagaa gtcttagaaa
    29161 ttgcaaaaga ctatcaagag gatgccaaga tggagaatcc aattaaagaa gcaatcacta
    29221 aatatttgga aatggaaatc ccgtataaat gggaagaagc tccagcgtgg gcgagacgtt
    29281 cttattatca gaactatcct gacagtaaat gtaatgagaa aaatttacag catttctata
    29341 atggagctac tatgcaacaa gagttttatt tagttgatag cgtactcacc gctgatattt
    29401 tggaagctgt ttttgataaa caagcaaagg atttattgaa tggtcgatca gatgcagaaa
    29461 ctaaaaagat tgccttgatt attagcagta ttcctggttg ggaacggaag caactgccaa
    29521 aaagaaataa acgacgagga ttttacaata agtcaaatgc ctatgaaaat aaaaatcgag
    29581 ctggaaagta caaaaagtaa cgggtagtaa cgggtcagta acacctagcc cgttactagt
    29641 ttacccttac tgccccaagg gatacagcaa tttggtaacg gggtaacacc ttatttcctt
    29701 aaaacttttg aaatagttag ctaatatgct ggggaataat gttactggat tttacccgtt
    29761 acctgttacc aaaacgctag aacccttgtc cccgttggga ttgagtggta acgggtacct
    29821 gttacgtagg tgttaccacg tgttacgaat caaggaggaa agtaaatgct caatattatt
    29881 tcaacaaaca aagcaccgaa ctttcaatat acagatgaaa tggatcggtt tctaatgaac
    29941 acgctggcat ttagtgtggg attggtaaca gaagattatt ccacatttga tccagaagta
    30001 ttaaagataa tggaagaaga acctgattgg ctgcaggagt cagtagcgtg gtgtcaatcg
    30061 ctagtagtgg gatcattggt agatagtggc aactatgatg atacaggtga gctaatggat
    30121 gaatttaatt gtctgcttaa cctatatgat cgagcgagac aacgagagct tacatcgaat
    30181 gaggataatt tgtttttaaa catccatgat aagttcttgg ctttgctact aacagatgat
    30241 gaattgataa ctaatttatt ggaggtagaa taattatgca tatggaaagt attagatact
    30301 tcgatggtga gaaacacatt gagtattcaa acactcaaga agatgtagac tttattagtt
    30361 ttgaggaaga taaaatcact aaagttaatt ttaaagacgg aacgtgcttg aagatagttt
    30421 cgccttatat tgaatacaaa tcaaaatggg aacaagatga ttctgacgaa cgagatcgct
    30481 ttggttggta atctatggca gttaaaaaac aatgtaatca tgctggatgt aaaacattga
    30541 ttgattatag gcagaagtat tgtgggaagc ataaagcaaa gcaaacggca attaagcgtg
    30601 aagaaagaaa gcaaagcgaa ggtaaatatt ttcaattcta tcaaagtaga acatggcgaa
    30661 aggcttcata cttgtatcgg ttaaatcatc cagtatgtga ggattgccta gaagaaggct
    30721 tgataaggaa agctgatgtt gtagatcaca aaattgaact aaaagatgat tggaatagac
    30781 ggttagatga aactaatttt cgttctttat gccatgcaca ccataattcc aagacagcca
    30841 atgaaagaca aagacgagaa aagagtaccc tttgagttaa gggggcatag tgtgaaactt
    30901 actgacaatc gatgcctact cattttggta caaataaccg ttgtgaaaag gcataagggt
    30961 aattacaaat aacgattaca cttgtaatca caagtgaaaa agtatataat gaaagtagga
    31021 gatttattga aaaatacaat tgttattact gataaaacag ggaaacagcg caccattgag
    31081 ttacctcatt ttggtcaagt gaccattcaa atgcagaacg gaaaaatcat ttatatagat
    31141 aaattagata aagaaaaatt ctgatcgaaa aacgaaggaa tgtgagctta attgcttacg
    31201 ttcctttttc ttttgtctga aaggaggaca acatgggaca ccctaaaata ttacacgata
    31261 caaaaggcaa tatatcaagt gaagaaaaag caatccgtgt ggacgctaga gaagaattgt
    31321 tcaagcagca accattaata aatatcacgc cccctgactg gatggcagcg agcgctagaa
    31381 gtgaatggaa tcgtatcgta ccaacattaa aaaaagatta tccattgagt gaagcagatt
    31441 atggttcatt ggtagcctat tgtttagcct ttgctcgaat gaaaacagcc gaagccgaga
    31501 taagaaaatc aggtacgttt atcacatgcg aaaacggagt aaagaaagcc aatccagcgg
    31561 ttcgggttca atcacaagct atgagtgatt tgaaaaaaca agccacctca ctaggtatga
    31621 ccttagaatc acgatcaaaa ctagctttga acaaggttaa aaatgatgaa cccgaagacc
    31681 cgttcaaaga gttgatgaat tcatgaatga ttacattgag aaagtcctat caggtaagct
    31741 gattgcaccc aagaaaatta tccaagcgtg tgagcgtcat ataagcgatt tggagcgttc
    31801 taagtcagat agcttccctt atgtgttcga tgaagaacaa gccaccaaag cgattaaatt
    31861 cattgagttg ctaccatcta cggacggtaa agcaatcaag atgttaggat ttcaaaaatt
    31921 cattctaggc agtctttatg gctggcgtac taaagaaggg aattacaggc gattcaatcg
    31981 agcgtttacc agtatgagcc gtaagaatgg gaaaacgtat atcgcaagtg gcatggctgc
    32041 caatgcgttg attatggaac aagaaccagc agaagcaagg caagtattgt ttgtaagtaa
    32101 cgctttgaaa caagctaaat tgggctatga tatgctgtct aattcactta gaaacgtggt
    32161 caagtctagt aagtttttaa gaccacaact gaaaattatg aactctaaga ttcagcactt
    32221 gccctctaat tcgttcgcta tggcactggc tagtgaaacc agcacgctag atgggtttgc
    32281 accaacaacc gcaattcttg acgagtggca cgaagcaaaa actcgtaaaa cgtacaacgt
    32341 cattaagtca ggaatgaccc aacaaaagaa cggcttattg tgtgttatta gtaccgctgg
    32401 gcttgattta aacgttccta tgtacgagga atacttattg ttagatcgtg tgctaaaagg
    32461 cgaagaacaa gccgacaggt actttatagc gatatgggaa ttggacgatc ccgaagaaat
    32521 tcacgatcaa gagaaatgga tcaaagccaa tccgattttt gaaagtgaag aaatcaaaaa
    32581 agtaatgatt ccaaccattc aagatgatgt gaaccttgct ttgaaacaaa ataaccttaa
    32641 ttctgtattg gtgaaaaact tcaatttatg gagacaagcg agtgaggaca gctatatgat
    32701 tgctgaagac tggcaggcaa ccgaagtaga accacaagat attacaggca agcccgttta
    32761 tatcggagtg gatttatcta aaacagatga tttaactagc gtttcatgga tcgtaccgct
    32821 agataacggc gaactttatt gtgattcaca tagctttgta gccaccaaat atgggcttca
    32881 agacaaagaa aagcgcgacg gtttgcctta tcgagaacta gaaaaagctg gtgagtgttc
    32941 cattactcaa ttagaaagtg gaatcgtgga ctatgaccaa gtatttcagt ttattcaaga
    33001 tttgattcaa gaaaatgatt tggaatgtat ggggatttgt tatgacccgt ataacgctaa
    33061 ttcgcttatc agtaaagctg aaaaagccaa ctacccaatg ttagaagtaa gacaaggaac
    33121 gattactcta aacgttccga cccgaacttt tagagaacaa gtttatgaag gcaacgttat
    33181 tcacaataaa aatacgattc tcacccatgc agtgaacaac gctattttaa aaacggataa
    33241 caacggcatt cagattaata aatcaaagaa cagtaacaaa attgatccaa tagctgcatt
    33301 aatcaatgcc tatgtgtttg caatggatta cttcaccaca acggaaggag cgaaagcaga
    33361 caatgaattt tatacaagtg aagaattttc tttctaatta cattcatacc gttcttttac
    33421 ttcttggatt ggtgtgtgtg ttggttgcta tcaccttact aacaaatgtc tactatggct
    33481 tgttagcgct gggcatagtg cttattgggg tagcggtcat gctaaataca gaacagaagg
    33541 gaggttaaaa gatggcattt tttaaagcga gacaaaatac aacgggagat cctttcctac
    33601 aacatgtggt atcaatccaa tcggatgatt acaccaccag ctttacaagc gttcgtgcat
    33661 taagaaatag tgatgtgttt gcagccgttc gaattattgc tagtgatatt gcttcaagtc
    33721 cgattcaatt ggtcaaaaac aatatgccac aagctgataa tgaactggtg aagttactaa
    33781 acgagaaacc taattcagaa atggatggtt ggcatttaaa gtttgctttg gcggtcaata
    33841 tgttgctaaa cggtaatagc tttgcagaga ttaaacgcaa cggtgaaaag gtagaagaaa
    33901 ttcacttatt acctaactca agtgtaacgg ttactcaatt agataatggt acgttgtctt
    33961 atcagattgg tgataaaaaa agacgtgtga agtctagcga tattttgcac ttcaaatatt
    34021 tcacccaaga tggtttaaca ggattaccac cactttatgc gttacgtgat gaactgaaaa
    34081 ttcaacaagc tggcaaccgc acattgcaca atttctttac tcgtggtgtc agtgggtcag
    34141 gtattttgaa ggttcataag tcagatttag acggatcagc aaagaacgca attcgtgaaa
    34201 agtttgaaga agccaacggt tcaagtagtg gagataatgc tcttagaacg atcattcttg
    34261 atgaaacaat ggactacaaa acattagaaa taaacaccga tgttttgaag cttatcaatt
    34321 ccaatgattg gaacacaaaa caaattgcca aggcgtttgg tgtaccgatt gagcgtttag
    34381 gcgttgaaaa tgaacattct agcaccgttc aaagtaatct ccagtatatc caaagtacgc
    34441 tgattcatta ctttaatgtg tttgtgagtg aattagatac gaaacttgaa accaatatcc
    34501 gctttaattc cgatcagtta ctagagactg atcctgaaac aaaagtaaaa aatgtattgg
    34561 atcaggtcaa agggtcactt ctcacgatta atgaggggcg gtcgaaaatg gggctacccc
    34621 ccatggatgg tggcgatcgt ttactagcaa gtttgaactt tacgtattta gatacgttgg
    34681 agaaatatca attaaaagaa caggaaggag ttacaccagt tgaataatga agaagaaaag
    34741 gaaaaacggc tgacagaaga agctgagcta acagccgatt ctcccaaagt agagaaagaa
    34801 aatgaaaaac aaccaacaga cggcaaaact atttcaggct atgcgttgaa attcgggcaa
    34861 ccgtcaaaag atttaggcgg ctttgtagaa gtgattacac ccgaagcatt aaaagaggtg
    34921 gatttatcaa atgtgttctt attatatgga cacgattatt caaagccact agctagtgtc
    34981 aaagcaggta cgttgaaatt gaatgtcgat gatacaggat tacattttga agcagagcta
    35041 accgacacga cctattcaaa tgatgtgtat gagaatatct caaaaggggt tgttgattct
    35101 atgtcctttg ggttcgtgct tggattagat tcattcgaca aaaaagaaga tggcacaatt
    35161 gaacgatcaa tagataaaat caaagcactt aatgaaatta gtgtcgtgac cgttcccgct
    35221 tatgattcat caaatgtcca agtaaataag cgttcttacg aatcgtttat gagtaacaac
    35281 caagcaaaac aaacaaacaa tagcttagaa tccacttcta aagcacaaaa ggagagtaat
    35341 aacatggaaa aaactttaat cgataacgaa aaaacagaat tgcgtgggta tgaagaatat
    35401 atccgttcac aaggcgaagt gcgtgatgga gtcactactg taaatgcagc ggcagttgtt
    35461 cccgaagaag taatcggtga agtctttgat ttgaaacgtt caaattataa cttagctcaa
    35521 tatgcaacag taaaaacagt atcaaatgga caaggtaaat atccagtagc aactaaccaa
    35581 caagcagtgt tagcaacaaa agctgaactt gctgaaattg gtgatattga cgctgaaatg
    35641 tttacttcag ttgattataa agtagaaact cgtgctggta agattgcctt atcaaatgag
    35701 gttgtggaag attcagcagt gaatattgta caagaggtca aagatcagtt agcgaaattg
    35761 gtagaaaaca ctgacaataa gcatatcatg gatttattaa aaacattcac taagaaaacg
    35821 gctgctacgt tggacgattt gaaacaacta tacaatgtgg cattagaccc agcattaaat
    35881 aaaatggtaa ttctaaacca aagcggatat taccacctag atacattgaa agattcagat
    35941 ggacgttaca ttttacaacc cgatgtgaca gcacctagtg gtaaatcatt attcggtatg
    36001 ccagtagtat tgattgcaga tacattgttt gccaatccta aagctggaac attccctatg
    36061 attatggggg atattgcaca atctatcttt gttgctcgta gaaatcaagt aacgactcaa
    36121 tgggaaaaat tcgattacta ctcacaagga cttgcagtga tcgttcgcaa cgattataag
    36181 aaaattgatc aaaatgcttc agtgtatatt gagtttacgc cagttgtaac accaaaagta
    36241 tagaaaaata ttgggcggcg gtttatccca ctgccttttt ttcattagga gagataaaat
    36301 ggtaattttg gatagcataa aaaaaagtat gcggatcgat cacactattg atgatgactt
    36361 tattcaacaa ttgattgata cagcaggcga atatataaaa agtgctattg atagtagcgc
    36421 aacggataaa gatatggata attatcagca atttgatttg gcggtgtcat tacttactca
    36481 acattggtat ttgaaccgtc aagaagccag cagcgaacgg ataccagtaa cggtacaagc
    36541 attagtacaa caaatgagag gtgcttatta tgccaatcat taagaatgta aatgaattga
    36601 ctgagagagt caagtttaaa aagacaaaac gggtaaaaga tgaagatggg cagatggtgg
    36661 atagtgaaga aactgtattt gaatgttggg ctaatgtgcg ttcacaaatg ctaaaagacg
    36721 tgcttgctag tgtaggtacc attcttgaag gaacgttgac gtttattatt cgatacgatc
    36781 aagattatga actaacaaac gatatgaaag tagcttggaa aaacaagagt tataaaatta
    36841 tttcaatcaa tgaaggaacg gcgtttaaag attatacaac gatcatagct aaacagattt
    36901 cttagaatga ttacacttgt aaacgttgtg ggtatttagt ataattaagg tagtaaatga
    36961 agggttagct gcctgaattt tgcaagactt agctagtcga aatctattgt tggactgaaa
    37021 attgtaggtg tggttgcaaa catgctggac taagaaagat gatagatgat gaacgttcaa
    37081 ttttaataca ggcatgctag gactgattgg atggttttgg caagctgatt gtgtagaaat
    37141 caaagaacac gtctgttgtg gacgtgtttt ttttgttata attcaaaaga ggtgatttaa
    37201 tggacaaagc agagtttaca aatttttatt ggaagtatta tctacattta gaaaatgagt
    37261 ttattaatac cacagatttt gtgatgctcg atgagaataa tttcaaaaca ttctctattg
    37321 aatatcaaaa actactacta gcgattggtt cggagtgtga gattattttc aaagaacttt
    37381 gcggatttga tagcagtagc aataaaagca taactgattt taaagctgtt attaaatcta
    37441 gcgaattaat gtgtttggat aatggtgtta gggttatgaa ttctttaaca ttgacttcac
    37501 taaaaccttt tggagatggg tggcctgaag atacaccaga ttggtggaaa atatataaca
    37561 gagtaaaaca tggtcgttct tcaaattata aggaagcaaa tttggaaaat gtgttgtatg
    37621 ctttagcaag tctatattta ttagaggaat atttgcataa gaaagtaata ctagaaggtg
    37681 aagtagactt tataagtcca gagtcaaatc tatttacgtt atcttgggaa agaaaacatt
    37741 cgtcgatgca aaaagtgacg tttgaaaaag tggattcaaa ttatgtagct ccactcattg
    37801 attttaaaga acaagaagaa atttaaaaac ccccacggct tattgaagtt tttgtatgcc
    37861 atattgtatg ccatttggtt taaatcgctt gaaaaatagt gatagataac tctaattcat
    37921 ttagtcataa acgctgtttt gataaccttt atagttcttt gatatgggtt tctcattcat
    37981 ggcggaagaa gaagaataga acaaaagaat gaattaataa gatataaaaa aagggattgt
    38041 gacaaacgtc ttgtcacaat ccctttttcc aaataaacga tgttcaatca gctgcccctc
    38101 ggcattcgct cgaaaaacgg cgaaatatga agaactattt tgccatttgt ttctctaata
    38161 atcaggtcaa aacgttgact tcacagtctc ttccttaaac ggtgttctcc tgccaactcc
    38221 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa ttgttccttg
    38281 tgactcggag ctgaacggca ggctcacgac ctctttctta aacggtgttc aaaagcttgc
    38341 atctgcggta ataagttcaa ccaaacgaaa attatgagga gctatttttg tttggctgtt
    38401 cttattactt gatgcaggac agctttttca caacctcttt tcgtttagca ctaaatttcc
    38461 ttaaaaagta gaaaagcttt cgaagcctcg tttgtttctt ttactgtata ataaaagaaa
    38521 agagtaacaa tcgctaaaag atataaacaa gagaaaggaa atacaagatg attggtctga
    38581 tttattttgt ggtgatcgtt ctggcgaaca cagttggcgc tatttccggg atgggtggag
    38641 gcgtgttgat caaaccgatt cttgatctta tcggcgccca ttctgttgca gggatttctt
    38701 tttactcaac agttgcggta ttcacgatgt ccattgtttc gactgtgcga caagtatcaa
    38761 gcgggaaatc actgaattgg cagattgtcg gttgggtatc tagtggagct gttgttggag
    38821 gagtagtcgg caacattgta ttcgaagttt ttttacagtt atttgaaaac gagaagcatg
    38881 tgcagctgat ccaaattttt ttgacggtat tgaccttggt ttttgcgttc ttctacacca
    38941 agcatcatca gccaaaattt catttgacgt catggacttg gtaccttata tgtggggggg
    39001 gttctgggct ttttagctag ttttttaggt attggtggtg gaccagtcaa tgtttctttg
    39061 ttgatgctga tgtttgcatt gccaataaaa gaagcaacac tatattctct atctacgatt
    39121 ttcttttctc aactggcaaa attggtaacg attgctttaa caagtagctt tatgcgcttt
    39181 gatttatcta tgttgtttta tgttatcccc gctgctgtta ttggaggttt gtggggagca
    39241 agatttagca gggttttgtc tccaaaaaaa gtgaccttta tttttcaagt aattgtcctc
    39301 gtggttcttt tggtcaattg ttacaatgcc tttgtcatct tacaaacatt aggagaatct
    39361 agactaacgc aagtcaaaat cactgaaaca tattgttcta aatgaaaaaa agagtatact
    39421 atgataaaaa aattcatttt aaaaaatcag aaattggctg gtgatttgga tgttacggga
    39481 aatgcgttta gaagatgcag aagcactacg tgaaatcaat gcggatcaat tagggtatga
    39541 tataccatta accgttacgg tgagacaaat gaagaaactg ctgaaggagc cagagaaaaa
    39601 ttttttcttg gtttatgaag atcctacttc taaaaaagca gctggctatg tacaagcgga
    39661 gtcctatgaa tcgattttta gcgatccaat gttcaatatt atggcacttg ctgtgtctaa
    39721 atatgctgaa aaaagaggca tcggaaaggc tttgatgact ggcgtagaag aagaagcgaa
    39781 aaaacgagga ataacgacaa taaggcttaa ttctgcagaa tacagagtag aagcacacca
    39841 attttatgaa cacatcggtt atcattccga taaaatgcaa aaaagatttt tgaaaatcct
    39901 gtaaatcttt atcaaaaaaa tacccgatca ccaagccgat acttgatgat caggtatttt
    39961 tgtttttttg actaagaaag taattgcatc aagaaaccag agatgatcaa aacgatggca
    40021 ccaccaagtc gattgcccat ttgagcaaaa gcaataagtt ccatccgatt agctgcagat
    40081 aagacggcta catttcctgt tccgcccatc gagttgttgc ataagccagc agtaatagtt
    40141 gattcaattg gatataaacc aaacaaacga ccgacaaatc ctgaaacaac tgaaatcgcg
    40201 ataacacttg tcagacataa gacaacaaat tgccatgtca aagcagaagc taatacattc
    40261 agattcagca acgcaatacc gataccggca agaacagctg ctgtcaagtt tttgacaatc
    40321 aagttattga acatgatagc tgcatcttca taatactctg gtaataggtt ggcgatttta
    40381 cagatgacaa cgataataat cataaatgcg taagcatgga ctttaggaac aaaatagtta
    40441 ccaatttgtc ctaagatgaa gaaagtcaat gataccatca aaccaacacc taactgtgga
    40501 atggaaggtt tcacagttcg aggttttcca tcgccaagat cgcctttttc cattaattta
    40561 ccattgccgt tgaattttgg cattgattgt ccagcacgag caactaatgc tgcaccgatg
    40621 atagctaaga catttgtcat tgctgtcgca ggaatcaggc gggaaatgat agaagaagca
    40681 tccgtaccta aaacatttgc ataaatcgtt gataaaggaa cggctccggc tccaacacca
    40741 ccagccatcg caggtaatga gatgtataaa acggaatttg caaaaccatt tccgataagc
    40801 atgcccacga gtcctacggc aaaaaaggag acgaccattg aaataagtgc aacgggaata
    40861 aaacgaacag aagctttcat caatagatta cggttcatac caaggatgct accagtaatc
    40921 aaggcagcaa tataaaagtc ccaaaagccc atattgctga caaagttttc tgtggatttc
    40981 actacatatt caggtaagat cccaaatgta gcaatcgcag cagaagcgaa gatcgcaaaa
    41041 acggaaccac cacccaaata acttttgacg atagggattt ttgtcccgat aaaatgaaat
    41101 aaattaccga aaataaccaa aactgcgatt ggtccaatca tattcgtcgg taatttccct
    41161 aaagccattg caatgatcag gacaaggacc atgatcaaat aaagtggcaa acttacgccg
    41221 gtgatttttg tggaccaaaa accttctttt ttcttttcct caactgcagt tgcagttgtt
    41281 gttttttgtg ccatttttac agatcctttc ttattctgta tcatatgaaa aaagccaaag
    41341 aaaagcgaca aaagagcagg agttggctgg ccgctgtttt gccgcagtcg cttttttctt
    41401 tttagacgtc atacaaagtt tctgtatgtt cttttactat aacgatctta atcttctaaa
    41461 gatttatatt caggaaccca tttagcagat tcaactgctt ctttgatatc cgtgatttct
    41521 tcacgattca agttttgtgc gacaactgat tttgcgacag tttcagcaat cgtttgagaa
    41581 aattcagtga tttttgctac tggaggcaag atagctgctc ctggttttgt tacatcaacg
    41641 attccgccta atgctcgact agcttgagaa atgatttcag catttactcg tgtagctgtt
    41701 gaagcaatca aaccaagtcc taaacctggg tacatcaaag cattatttgc ttgaccgatt
    41761 tggtaagtta caccattata ttcaacatca gcagcaggga tacctgttcc aactaatgct
    41821 tttccgtccg tccattctat taaatctttt gcttttgctt ctgccaattt tgttggatta
    41881 gacaaaggca tgatgatcgg acgtggtgta tgagcagcca tttctttcac gatttcttca
    41941 gtgaatgctc caggttgggt agaagtacca atcatgattg tcggatggat cgcttttaca
    42001 actgcttcta agtttgtcaa ttcatcagca ttagtgaaat ctgagcgttt gcgagcgaat
    42061 gggatttgac caggagtcaa tccttctgta tcttcaaaca ataaaccttg tttatctact
    42121 tgatagaaat gttggcgtgc ttcttcttca ggtacacctt gacggatcat ctcatctaaa
    42181 aggatgtttg cgatacccac accagcagta ccagcaccaa atgtcaaaat tatttgatct
    42241 ttcaattgtt caccggatat attcaatccg cctaatacac ctgctaggac aacaattcct
    42301 gtaccttgga tatcgtcgtt gaaagtagtg atttgatctt cgtatttttc taagatcgct
    42361 gcagcatttc ctcgtccaaa atcttcccaa tgcaataaca attctgggaa taattcggta
    42421 gctgattgaa cgaattgatc gatgaattca tagtaagttt cgccttctac acgagcatga
    42481 cgattgccta agtataaggg attattcaat aattcttcat tattagtacc agcgtcgatc
    42541 gatacaggca aaacttgtgc tggattaata ccagcagcag cagtatagac catcaatttg
    42601 ccgatagcga tgtcaacacc gttcacaccc cagtcgccca tacctaagat tccttctgca
    42661 tcagtcacaa caatcaaacg aatgtctcgt ccatctgccg cgttcttcaa acttgctttg
    42721 atgttttctg gttcgtcgat tgaaagaaat gcagcatctt gcggtttcaa gaaaatttcg
    42781 ttgtattgtt cgattgaatc cgcaacgaca ggatcataaa cgacaggcat gaattcaact
    42841 aaatgttcgc ccatcaattt ataaaacaat gtccgattcg tattgaatag attcattaag
    42901 aagatacgtt tttccaaatc tgtttgtttg cttaagtatt gaccataagc ttgaacagat
    42961 tgttgttcca gtgtttggac agtacttgga agcataccag tgattccata ttttgcccgt
    43021 tcttcttttg taaaagctgt tcctttattt agaaaaggat tatttaataa gtttaaccct
    43081 gtaagcatga atgattcctc ctaagtttta atacaaacga agtatagaag tcagatgatg
    43141 atatagtcaa aaagagcagg tatgataaat tttatttata tgtaatatag ccaaaagaaa
    43201 tcaaaatagc agtatttgtt ctttttatat cgacaatcct tgcttgaata ctatgcgtat
    43261 ctcttatatg ctagagaaag aaaagtagaa gaataaggga tagatggaat ggataaatag
    43321 gatttctgag gaaaaaagca catgatagcg ttgataggag tcacctatgt caggtgataa
    43381 aaatacataa gtagaaaatt aagaggagga agagaaaatg aaagaagttc aattgattag
    43441 aaaaagcgaa ctatcagaag gcggttgcaa tgcatgtggc gtagtagaag cgacaagcta
    43501 tacattaaaa ttagattcca atcaagcaat aatctctgaa ctgacagtag gcggtcttgt
    43561 ggacagtctg gcgcttgctg aaggttttac tggagaagat atttatgaga tgttttcaga
    43621 agtccgtcag ttaaaaaaag gagaaaaatg catagaggtt catcatgaga gcccgaatgt
    43681 gaggttcaaa cgtggtgata atgagatgat cttcaagaat catgtgtctg atcacactga
    43741 actatatgaa atcgtgaatc aaatactgtc tgggattttt gaattaggac cttatgagtt
    43801 caaggaagaa aacggtaatc caaaattaaa tgaagaatgg caagaaacga ttgaaacaca
    43861 aagaaacaat ccacacttat ttcaatagtc ttccacagta tttcgtaaaa atgttgatga
    43921 aaaagaaaca ccgtgaatca gaggtcgtga aaaacctgcc ctgcatcaag taataagaac
    43981 agccaaacaa aaatagctcc tcctattttt cgtttggttg ggcttattac cgcagatgca
    44041 agtttttgaa caccgtttaa ggaagagact gtgaagtcaa cgttctgacc tgagtattag
    44101 agagacaaat ggcaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt
    44161 cagctgattg aacaccgttt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgagtagt
    44221 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttgact taataccgag
    44281 aaaatggctt ttgaacaccg tttattcaaa aaaagggact ttgacactac ttttgtcaaa
    44341 gtcccatcgg ttttttatct atttttatta caatcatttc ttccagagta gtgacatctg
    44401 gaatggctga aatgtaaaca gattatttgc ctgtatcaat gtcgaagagt aatataaaaa
    44461 gtgtaaacgc tatcatattg ttcatcattc aaattttata gagagaaggc attgagatgg
    44521 aaaaaacata tcaagcaggt acaaatgaag gaatagtaga ttttatcaat atggaagatt
    44581 tagaaatcgc ggcttcacaa gtgattccag ctggtggtta tggttacatc agcagtggtg
    44641 ctggcgactt atttacttat caagaaaatg aacgggcatt caatcatcgt ttgattattc
    44701 ctcatgtatt aagagatgta gaattacccg atacaactac acattttgac gaagaaatgc
    44761 tgactgcacc aatcatcatg gcaccagttg ctgctcatgg attagctcat gtgaaggcgg
    44821 agaaagcttc tgcaaaaggt gtagcagatt ttgggacgat ctatacagca agttcttacg
    44881 cttcttgtac tttagaagag ataagagaag caggcgggga gaaagcaccg caatggtttc
    44941 aattttatat gagcaaagat aatggaatca atttggatat tcttgaagtg gccaagcgta
    45001 atggagcaaa agcaatcgta ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag
    45061 accgcagaaa cgggtttacc ttcccgctgc caatgccgat cgttcaggct tatcaatcag
    45121 gagtaggcca aacgatggat gctgtatata aatcttccaa acaaaaactt agtccaaaag
    45181 atgtagaatt tatcgctgct cattctgatt tgccagtcta cgtgaaaggt gttcaatcag
    45241 aagaagatgt ctatcgatca ttagaatctg gtgctggtgg aatttgggta tctaaccatg
    45301 gtggccgtca attagatggg gggcctgctg cgtttgattc gttgcaatat gtggcagaag
    45361 cagtagataa acgtgtacca atcgtgtttg acagcggtgt acgacgtgga caacatgttt
    45421 tcaaagcaat cgcatcaggt gccgatcttg ttgctattgg cagacccgtt atttatggat
    45481 tgtctttagg aggaagtaca ggagtccatc aagtgtttga tttctttaaa acagaattgg
    45541 aaatggtcat gcaattagct ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc
    45601 gtgagaatcg ttttatttaa gaaataaatg acgaaaaaaa taggagtgcg gcagatgatg
    45661 ccgtactcct atttttatag ttcaaggatg tcactaatag aataatttga aacgaaaaaa
    45721 taaaattgaa atattttttc atacttaata aaaaaggaag aatattcatg taaacgattt
    45781 tattgttttt ttctctttta tacaaaaagg gtcataattt caagtaagcg gatgaattca
    45841 tgaggatttt ttgcatactg tctctactat acttaggtca ctaagaaaaa ggagtgattt
    45901 attatgaaaa agaacatgat tgggacatta ttactttcaa cacttcttct aggcggcttg
    45961 gcaacaccag cttttgctga gggtcaagcg acttcaaaag gagatattac ctttactgaa
    46021 ccaacaaata ccgttgagcc tcttaaccct actgatcctt caaaaccagt agagccagca
    46081 gatccggaaa atccagcaac tggccagaca ggttctttaa cattggatgt agttcctgaa
    46141 ttgccatttg gtacacacga gattgaaagc ggaacaaaaa cgtatcaagt agacgcttca
    46201 aaaaacgata caccttatct ccaagtatca gatcgtcgag gtgtaggtgc agatggccaa
    46261 gcacaagggt ggaacgtgac agtttcggtt tcagattttg taaacggttc acaagttttg
    46321 caaggcgctg aattagattt tggaacatcc actgttaaaa gtacgtcaga taacgaatcc
    46381 actggaccaa ctagtcaaac tgtaactggg ttgagtaaag caagtgcagc gacaccaata
    46441 tttacagcag caaaagatca aggtctggga acatggcttt ctgtttatga tccagcaaat
    46501 atcacactga aagtgccgaa agcagcagct ggaacattca cggctgattt gacttggaac
    46561 ctcgtggctg gcccagtagc atagagtatg aaataaattc aacaaactgc ggtgaaaata
    46621 ctataggtga aggatttttt accgcagttg ttgttgaaag gatgtggtgg acaatgaatc
    46681 aaagtaaacg actgtttgtt tcgttttttt ctattttaag tctatttttc atttttccgt
    46741 caatcagcca agcaaaggat tcagcaggag actttggaat caaaccggta tttccagaga
    46801 accaaataga taaagcaatc ggttattttg atttattagt ggcaccggaa caaaatcaga
    46861 cactagaagt aattatcagc aattcttcag atgaagaacg aacatttgag gtctctgtca
    46921 atccggcggt aacgagtgat ggtggaacga tcgattatag tcaaaaaaac ccaacgttag
    46981 atgagacatt gccgtttgat gtccgtgacg ttttgctcat tgcgaaaaag gaaatcaatg
    47041 tatcagcaca tgcagaaaca actataccta tagaagtgaa aatcccagca aaaagcttta
    47101 agggacgtgt attagcgggt attcatgttt cacctaaaga agaagctgaa acagaaaatg
    47161 ctacagaagg agcacaaatc aaaaatcgta ttgcttataa tctcgcagtc gttttacaag
    47221 aaagtcaaga gacgatagaa ccggatctga aacttttatc aggagatttg gacgaggtca
    47281 acgcaaagcc aactgttcag cttcgtttcc aaaatcctca gcctaggatt atcagcaatt
    47341 tgatatttac gagcaagatc ttttatgaaa atcagcttta tattgaaaat acgtctaatg
    47401 aatttttagt tgcaccaaat tcgaattttc acttgaatct tgatttagct ggtgataaag
    47461 caaaagccgg cgattaccgc gcagaaatca tagcaaaatc aggcgacagt aatgaatggc
    47521 gatttacaca aaactttacg atcaaaaaag aaaaagcaca aaaagtaaat gaaaattccg
    47581 tttttgcagt acaagaacaa tctttcccat ggctttatgc aggattggtt gctgcagttc
    47641 tagtgttatg gttgattttg ctgtttttgt gggtccacaa gaaaaagaaa aaaggagaaa
    47701 caacatgatg aggtgatttc gttgaaaaca tcgatcttat tgggagggat tattgcaagt
    47761 ttgctgttct tcagctggca agagccagtc caagcagata ccaaaagttc aggtacgatt
    47821 acattttatg atctaagtcc agttccgcat ccaagtggtt cagtcaatgg cgcatcaaaa
    47881 cagcagacac agctaccgat ttataaagaa tctaatcaac atggcgtatt cccggagacg
    47941 aatgaagaaa agatccgttt tctttatttg atcattggta tagaattatg gcttcttgcg
    48001 tggttgttgt tatggagaag aaaggaaaag gaggatgaac atgttgctac aaaataaatt
    48061 ttcatttttt cgctccttct tttttacttc gctttttgtt ttaagtatat ttatcttttc
    48121 accttcggca gaagctgttg gagcaacgcc tcctgccatt tcttatgatc catacacgta
    48181 tgaaaatatc gtacctccaa atggtggacg tagtgcagtc aatggcggga agcttgtcca
    48241 actaactggg gctaaccaat tgcctaaatt gactgctact tatttgaacg atggttcaaa
    48301 tgatccttgg aaaggtggca cggtccgttt tcattgggaa agccatgtga aactaacaaa
    48361 tgtacttgat ttggtgaata tcactgcaca aggatttgca tttgtggcga tcatcaattt
    48421 gccggaacat gtatcaagca gtgatgtatt aaaagcaatt gactggaaca acgcctactt
    48481 gtatattgcc ggtgccccca tcaaggttac tagcggtagt ttattatcag caggaagcca
    48541 taccttgcgt tttgggatgg ggtcgtggga tacaaatact ctattggcag atattattgc
    48601 tattattacg cgaggcgaat tcaacgtgga cgatatcccg cttcaatttg atgttacagc
    48661 taatgtcgcg aagatgacag aaaacggaga taaatatgat acgagtccaa acaaaatttt
    48721 gaccaagggt aaattacaac cttctatgtc taaaaaggcc gttttttctg ttgattttta
    48781 tgattcgaat aatattatcc aagatcgtat gttcaattcg attttagctc cagctgcagg
    48841 tagattttat cctaaattaa ctggcggaaa cttgaatcca ggagcatatg cattttctgc
    48901 aagtgcagag ataaatacgt ggaatagtta catctcaaca agtgattcaa caggaaaata
    48961 taatacattt gctgatacca cagaatacgt ggatggaaat aaccagaacc ttccaggctc
    49021 taccaatagt cggacattga cgatggggct gactgaagga cgttttgatc agttgccatt
    49081 tagtcgcttt gatcgcgtag tcaactattt tacaggaaaa aacgttacac aaggagcaat
    49141 actgactcat actccagcaa gcacaagtgg attgaatcag acagccaata tcgtgtacag
    49201 tgggaaagat gcgcaaggcg gattgctttc tcctgttgct ttaaaggtat tggagcagta
    49261 caagctagac ggaacggttt taccaacaaa cttaacagcc gatagtcagt ggggaaagct
    49321 cctttcttct gcatcgtacc aagtcacggc caattggtca gcaactgaac taaaaaaggg
    49381 ttcgatccat tacaggcttt attcgaaagg aacccaaaaa ttagcgacag gatggacaga
    49441 tcaacttttc caaacgatcc aatcaaataa cgggggtgta aatacagcta cttctacact
    49501 acctgcattg gcttcagggc aatattattt tgattatcgc ttggtggacg atgtacttac
    49561 taacatgtat ccgtcttttg cgtacaaatg gcagtctgaa caagctggaa tcaccactct
    49621 tcctcaaata acagtggcta attttccatc tgtgagtgca gatagtcagc taaaaaacct
    49681 tagcagacaa cctgagacag gagatcccct gtctgctcta tcaggagata cgattcaaga
    49741 aaatgtgaca ttcacgttaa caaaatctgg ggatgcgata accgacaaaa aaatcacgat
    49801 ctcacttcct gaaaatacta cttatatcaa tggttcctta aaattaaatg gtacaactat
    49861 cgcagatacc ggcattcaac aaggagtttc tgtcccagca gatctattga gcaaaatagg
    49921 agataccatt catcttactt acaactatca attaaatact gtagatactt ctgtacaatc
    49981 cgtcagcata ctgacaaagg cagctgtact ttccagcaat attacattag cagatggtgc
    50041 taagcttcct aacccagttg tccaaacatc tgctaaaaca attcttgtcc ccaaacaaga
    50101 attaacatta gttaatgtac cagatgattt tacatttggt aacgatctcc ccaaaccgtt
    50161 gaagacaagt tattatgaag caaaaggcga tttttctttt gatgtgcggg atactcggct
    50221 tccaagcacg tctccttggc aactgacagg gacattgact tctttattta agaatgatca
    50281 aggtcaagaa ttaagcggaa caaaattata cttcaatcat tctggatcaa aacaattgat
    50341 tcagcaaggt caaaacacgt tgatttatga gagtgatgga acagctaaag gagaggttct
    50401 cgttgacttt cccgataccg atggtttact cttagaggta aatagcagta cgaatgctca
    50461 acctggcgca acgtatcaag gcatggtcac atgggaactg acagctgggc caaccagcta
    50521 agtgattaga atttgtatgg atctgtgata actgtcacag atcctctttt tcaaggatga
    50581 gaaggaaact tctgctgtta aaatcagaaa tgcttggagg agtggacttg gaacttttca
    50641 gtttgcttga aaaaaatgaa caacggcaaa tcaaactttt ccaactttta ttagaggaaa
    50701 aaggagagat ttccgttaat aacgtactta cccgcttatc agttgatcgg gctaccttga
    50761 aagaagatct gttttattta caagctaact tggaaaatat caaacaattg gaaattgtta
    50821 tccaagaagg gaatatttta ttaatcagac atggaaatat agcagcagtc gatgtttatt
    50881 atcattgcta cttgcgctat tctacaaaat atcagatact tctttatctg ttggaaaatg
    50941 gctcatttga tcggcagaaa cttgtcatgg aacttaatat cagttcggct actttgtctc
    51001 gcagagtgaa ggaattaaat gattctttaa atgagtttca tctgaaaata aaaagcgggc
    51061 ggctgaccgg tccagaaagt caaatttgtt atttttattt ccaattattt tggtttggac
    51121 gtccttatct tgaaaacaaa aaagaataca gtgatttatc aggaaacact tttttagatc
    51181 tgctgcaaaa agagtttagt tttactatta cagaagaagg acgattgaaa tttgaaatat
    51241 ggctttccat tatgaagaag cgaatgcgct gtacgaaaaa atccaaacca gattatagca
    51301 atcttcctgt tggattgttt caaacgacac aatttttatt ggacctccat acatttttga
    51361 cccgttactt ctttcaatat gcctttcctt ggaatcaaaa tgaaacgttg gttttttatc
    51421 ttttcatgat cagtaacttt acattagata cacataactc gcatgtccag gagtttttga
    51481 taagcgaaaa atacaaagac gacgtagtag ctcaattgaa tcgaacgttt aaacaatcga
    51541 tagaagaata tttctctttt tatgtttttc cagaagagat ttcgcaaaag attgcgttga
    51601 cagtgatgca aaatcacgct cattttgcct attttagagg atggatgaat atttttggcc
    51661 aagaaggatt aaaacagcgg ttaactgaag tagaagataa acaattcctg gttttatgtg
    51721 aaagattggt cagacaaaca tttgctattg ttgattttac tgaagtagac gaggaaaatg
    51781 cccgctttga attatttgct cgctatgcat ctattttgcg gacgctgatc aagcagttag
    51841 aattacgtct gcaaattgct tatgatcttc cttatgaaag gctaatggcg gatatcgtga
    51901 aagaaatcat ccaggaacaa ttgctgaata cgatcaaata tgatttagtc gaatacgaga
    51961 aagaaaagca gtatgatatc attttgacta gccaattgaa agagtacccg tcacagaagt
    52021 ccgctaaagt ttttgtattc actagcaacg aatataatta tgatttcccc tatttgaacc
    52081 aatttttaaa agaatgttat ttagaaaaat tgaatttaag gatgaataaa acatgaaaat
    52141 cgtgatcatt ggtgcttccc atgcaggcat cacagctgcc ctaaatctaa gaaaacttca
    52201 accgaaagca gaagttttac tgatcgatga agatcataaa gatggactag gctatgtttc
    52261 aaatggaatc aacctttatt taaaaggaaa gatacgtagt ttagctgaag ttgcccataa
    52321 catgcggact cttcaagaaa gtggggcaaa actgatcacc gaatggagag taactgaact
    52381 tgatccagac aaacatcagc tcatcctcgc atccaaagaa ggaaaaaacg agaccataac
    52441 ctatgataaa ttaatcgtag caacaggttc ctctcctgtc accttgtata aacaaattga
    52501 agctgaaaat gtctacacct ataaaaatct agtgcaatct aagcaagtac ttgccgcttt
    52561 aaaagaagca aaagaagtag tgatcttcgg tgctggttat attggattgg aattagcaga
    52621 tgctttgaga aataaaggat acattattca tctagttgat tatatgccga atgttttatc
    52681 aagatatttc gataaggata tgatcaacag ttttcaaaat cagctgcaaa ctaagcaaat
    52741 caatttttat ccaaatgaat tcttgatcga ttggaaaaaa tcagaagaga aagtagtatc
    52801 cgtacaactt ctatctcaag caataaaggc agatatggtc attttttctg cccaaactcg
    52861 tcctaatacg acattgttaa aagaaaaagt ggctttgtat gaggatgaga ctgtgatagt
    52921 caatgaatat ctgcagacaa gtgatccgga catctatgcg attggagata tcgtcccagt
    52981 cagttttgac aaaaataaac ggcatctctt tttaccttta gttactcgag ctgtacatat
    53041 ggcaagagca gtagcgctaa ctttgagtgg tcagccaacc gcttacgacc tcagacaaaa
    53101 gataacagca gcagtcatta tagactactt tttaggaact gtagggctga cagaagatga
    53161 agcgccattc ttggaacaaa ataccggttc gtgttcagga gaatttgatc tatttccaca
    53221 atacgatgaa gaaaacaaaa cagtaaatgc aaagctgatc taccatccgg atacattaga
    53281 aataattggc ggacagctga taagccaaga attcttatta tcagatttga acctgcttgc
    53341 ggatatcgtg aaacacaaaa ctacgattcc acagctagca gttgaggatt ttggatttct
    53401 gtctgaacac acctctcgtt ttcactacct caatgagtta gcttttaaag tactagctaa
    53461 gagagcaaat agcaatagag tagataaaag actgtgagaa atatcacggt cttttattgt
    53521 gtggggatat aataaatatt aatcaatcga gaataaaaaa ttcatataat gaaatagcgg
    53581 aagaattttt tgagcataat attcgccaaa aatttatcaa gtgataaaat tgttgtataa
    53641 aatgattatt tcaggaggag gagttaaatg tttttagcat taaatgaaat tatgcattca
    53701 aagttacgtt atgcgttagt tgcaggggtg atgtttttga tcgcctacct ggtatttttt
    53761 ttgactgggt tagcttatgg cttagctcaa gataatcgaa cagcagtcga caagtgggag
    53821 gcagattcga ttgtgctttc gaaagatgca aattcaaatc tagggatgtc catgattact
    53881 aagaagatag cagaagaagt cgaaggtggg aaagtggcgt atcttgccca gacacctgga
    53941 gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca atgttagctt cttcggtatc
    54001 gataaaaatc aattcatcat gcccaatctt gttgaaggaa aagcttttga taacgatgat
    54061 gaggcagtag gcgatattag tttgaaagaa gaatatggtc tagccgtagg agatacagtt
    54121 aagttatctg gtagtgacaa gacgtttaaa ctaactggct ttacggatca tgccaaattc
    54181 aatgtctcac cagttctcta tacaaccatc aatgcttacc aagagatccg cttcgaaaaa
    54241 gaagatacaa gtgaaaatgc acggattaac gcaatagttg tcagaggtaa aatcagtaat
    54301 ttacctgaag atttagaaca gatcaaaatt tctaaattca tcaatgaatt acctggatat
    54361 aatgcgcaag tattgacttt tggatttatg atcggatttt tgatcgttat tgcggctatt
    54421 gtgatcggga ttttcattta tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg
    54481 aaagcacaag ggatcacagg aggatttatt gcgcgttctg tagtggcaca aacattcatt
    54541 ttatcatttg ttggtattct tcttggtcta ttaggaacag tgggaacatc ccttgttcta
    54601 cctgatgcgg ttccattcca aagcaattgg ctgttttttg gcgtcattag cttgttgatg
    54661 ctggtagtag ctgtgttagg cgcactattt tctgttcgga caatcgtaaa aatcgatccg
    54721 ttgaaggcaa ttggataaga aggaggaaga gcatgaaagc aatagaattt aaagaagtag
    54781 aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca accatgagta
    54841 tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt
    54901 tgactttggc aggcggattg cagacgccta gcagtggctc tgtatttatc aatgatcaag
    54961 aattcagtaa tcaaaaagaa agtgaacgcg ttaaactacg ttttaaagaa attggcttta
    55021 ttttgcaagc ttccaatctg gttccttttc tgacagtaaa aaagcagtta ttattagtag
    55081 ataagatcaa acatgaacat cgtcaaaaag aagcaaaaga attatttgag cagcttggcg
    55141 tggataagtt gatcaacaag tatccagaag aactatctgg tggagaacgg caaagggtcg
    55201 caatcgctcg tgcattgtac aatgatccgt ccattattct agcagatgaa ccaactgcta
    55261 gtttggattc agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca
    55321 aaaataaagc aacgatcatg gttactcacg atacacggct gattgacaaa tgcgatagaa
    55381 tatttatgat ggaagatggt gtattgaaag aaaaagaata accaaaaagc aggtggaaaa
    55441 atggtccatc gatacaacaa agatacacag caaaaaattt tgaaagaagc gactgacttg
    55501 tttatgtcca agggatatct cggcacttca acaagagaga tcgcacaaaa agttgggatc
    55561 acccaaccca atctctatca ttatttcggt gataaagaaa aactatatac ggcagtttta
    55621 gaaaaccact taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg
    55681 ggatttcaag taaccttgac aaaaatggcg cagtttttga ttgagacaca tctggtcgat
    55741 ttgtttctga tgcttcacga tcttgaaagc aatctttcaa aggaaacaag agatcatctt
    55801 ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa
    55861 tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta
    55921 gcaccttaca tcactcggtc gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg
    55981 atcgatttat atttgaatgg tgtattagga gaagcgtaaa gctgttatat agggagaaaa
    56041 atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgtttt ttttatatag
    56101 gtagtttttc ataaatacaa atttgttatt ctctcatgat tatcagggta tatttttatt
    56161 tattatgtcg gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta
    56221 gaatgaagga aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc
    56281 aggatatgcc aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat
    56341 cttttacagc cccttttttg cgtacttaag ctgattttaa gttttgaccc ttagtctgga
    56401 tctacgaggt gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt
    56461 atcattatta tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg
    56521 gtcagcagta cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa
    56581 gagacattaa tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact
    56641 accccacttg caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat
    56701 gcttatcaga tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc
    56761 aaagtcacaa agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta
    56821 gatcctgggc atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa
    56881 aaagatataa cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca
    56941 acggtttatt tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac
    57001 agtgaagaaa aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg
    57061 aatgaagcaa ctggaatcac gacatattat tactatggac aagaggaaac attagcacag
    57121 acaattgctg actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac
    57181 tatcaagttc ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat
    57241 aatgatagtg atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt
    57301 ttgacacaag cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag
    57361 ctcctcatat ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt tgaacaccgt
    57421 ttaaggaaga ggttgtgagc ctgccgttta actccgcgtc acaaggagca atttcaaaaa
    57481 acagcttctc atgtttgatg actattgtga cttgtgaccg aggagttggc atgagaacac
    57541 cgtttattcg gaacaaggga ctgtgacatg acttttgtca cagttccatt atttttttag
    57601 cagcttcaaa gctttttctg tattggcaaa atgaagtttg gcatattttt caagcatagg
    57661 catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc cagctcctga
    57721 tggaagggtg attccagctt cagctgatgc tttgtgtaac tcttccaaaa agctggcacg
    57781 actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag taacgaagta
    57841 cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat ttgcttccgg
    57901 cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt ttagcagcaa
    57961 atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt ttggttggat
    58021 ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt tttttaagat
    58081 gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa caccaagttt
    58141 acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta gcggaccaaa
    58201 atagctgcca tttccgacct catcactacc taaaacggat aattgactga tattttttgg
    58261 caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc cccatttatc
    58321 agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat aagcggtaat
    58381 ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat ttaacaaata
    58441 agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga taatgacgtt
    58501 gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac gacttttcgt
    58561 tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat agttaagtat
    58621 gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact aaataaaact
    58681 ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta caaagcagtg
    58741 attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat ggatatcgta
    58801 acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca aatggataat
    58861 gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa
    58921 agaatcttgg agctggaaga agaaacagca gaattaaaga aaaaaatgat caaatttaca
    58981 gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg ggaagtattg
    59041 aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca acagatttta
    59101 aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc ttttatcaaa
    59161 ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta gcatgctgag tttgctgatt
    59221 ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc tttgcaaatc
    59281 atattcgcga ttggatatgt attgtcattt attgctgccc agcattttta caaaccatta
    59341 gcaagtcatt tggaattata cattccatac ccagctgtca ccccaacatc aaagttagca
    59401 ttttttgatc aggtatttgc ttttcaccta gatgaagctt tttatgcagg aactgctttt
    59461 ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt tgtccatgga
    59521 ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat cctgtcacta
    59581 atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt tccattagtg
    59641 tggtccaaaa tcaattcggc gagcatagtt tcgctcgttt tatcgttgag agaacgccat
    59701 ttcttaccaa caagatgtat gagctttggg taacgaatat tttgaattaa ggaaaatagt
    59761 gctggaaaat atttcgtttt ttgtacagag tagtttattt aagaaggctg cgtagcgtat
    59821 gtccggcaca gctctttttt catagtgaaa gataaggtga taaaatgaat aaccgaatcc
    59881 ttgaaacatt ggaatttgaa aaagtcaaac agatggtcag acaatttgta gtgactgcac
    59941 aaggaaaaga agaactggca gaattggtac cagtgagtga gaaacaaacg atcacaaact
    60001 ggcttcaaga aactgaggat ggattgaaag tgcaacgtct gcgaggcggg atccctatac
    60061 ctaaacttga aaatatccgt ccccatatga aacggatcga gatcggagca gacttgaatg
    60121 gagtggagct tgcacaagtt tccagagtat tgtctacgac aagcgaattg aatcgtttca
    60181 ttgatgatct ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt
    60241 tagtcacgat ccctgttttg agccgccgtt taaaggaagc aatcgatgaa gacggacgcg
    60301 tgactgatga cgcttctcct gagttaaaga gcataagaca gaacattcgg cgaagtgaac
    60361 aagctgttcg tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg
    60421 atgcgatcat caccatgcga aatgaccgct acgttatccc agtcaaacaa gaataccgcg
    60481 gtgtatttgg cggtgtagtc cacgatcaaa gtgcttcagg acagacttta tttattgaac
    60541 caaaacaagt ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg
    60601 agattcaacg tattttaagt gaactttctg ctgaacttgt tccccatcgt caagaaatca
    60661 tccacaatgc ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta
    60721 aagaagtaaa agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag
    60781 caagacatcc actgatcgat caggaaaaag tagtttctaa tgacatcaca atcggaaaag
    60841 actatcaagc catcgtcatt acaggaccta acacaggtgg gaaaacgatc acgttgaaga
    60901 ctttaggtct gttacaactg atggggcaag caggattacc gattcctgca ggtgaagaaa
    60961 gtcaaatcgg tatttttgag gaagtatttg cggatatagg agacgaacag tcgattgagc
    61021 aaagtctatc gaccttttct tctcatatga cgaacactgt agatatttta tcaaaagtca
    61081 atgaaaaaag tctggtatta tttgacgagc tcggtgcagg aacagatccg caagaaggtg
    61141 ctgctctagc aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa
    61201 caacgcatta tcctgaattg aaagtttatg gatacaatcg ggcaaatacg attaatgcca
    61261 gcatggagtt cgatgtggat acactaagtc caacgtatcg tttgctgatt ggcgttcctg
    61321 gacgaagcaa tgcgtttgaa atctctagcc gattaggtct tgatacagaa gttattgatg
    61381 aagcgaagca gttgatgaat gatgagagcc aagatttaaa cgaaatgatc acagatttag
    61441 agaatcgccg gaaaatggca gagaccgaat atctggaaat gcgtcatttt gtttctgagg
    61501 cacaagaact tcacgatgat ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa
    61561 aagaaatgga aaaagcaaag aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag
    61621 cagaaaaaat cattgctgat attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga
    61681 aagagcatca gttgatcgat gcaaagaccc aattagcaaa tctacatcaa gaagaaactt
    61741 tgaagaaaaa taaagtattg aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg
    61801 aagtgttggt tacgacttac ggccaaagag ggacgctttt acgtaaaaac gggaatcagt
    61861 ggcaagtaga aatcggtata ttaaaaatga atgtgtccga agacgagctg acgcctgttg
    61921 ctcctcaaaa agagccgaca caacgagtta tccatgctgt acgatctgaa tctagtagtc
    61981 acgtgccgaa tcaattggac cttcgaggaa aacgatacga agaagcgcta agtgaggtag
    62041 atcaatattt ggattctgcc attctagctg gttatcctca agtgactatt gtccacggaa
    62101 aaggaacagg cgcacttcgc aagggaatta ctgattatct taaaaaccat cggagcgtca
    62161 aaagctttga gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta
    62221 agtaaagctt actaaaggta agcagatggt tcgctatcca agtatagaaa tgctatacta
    62281 atcatagatt ttaaaaatgg aggtctttga tatgtcacaa gtaattacag ataagacatt
    62341 taatgaagaa acagataaag gacttgtatt gattgatttt tgggcaactt ggtgtggtcc
    62401 atgtcgtatg caagcaccga ttttggatca gttggaacaa gaatatgatg aagaagaatt
    62461 cagaattgct aaaatggatg tggatgaaaa tccagaaact cctcaacaat tcggtatcat
    62521 gagcattcca acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt
    62581 tcattcaaaa gaacaactac gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa
    62641 aataagctga gggtgggact gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa
    62701 ggttattcaa attaatattt tttgagtata attacgttgt caggctggat tgtttttttg
    62761 acagacatgc tataataact ttaattgaat atgaagggga gtaccagatg aacgaacgaa
    62821 tcaaaaacaa attagcttta cttcctgatc agcccggctg ttacttgatg aaagataaaa
    62881 atggaacgat catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt
    62941 ttactggcag ccataatacg aaaacggaac ggcttgttag tgaaatcgaa gactttgaat
    63001 acatcgttac agaatcgaat atcgaagcat tgctcttaga gatcaatctg ataaagaaaa
    63061 atgatcctaa atacaacatt atgttaaagg atgataaaac ctatcctttc cttaagataa
    63121 cgaatgaaaa atatccccgt ttggtgatta ctcgtaaagt actaaaagac aaagcactgt
    63181 attttggacc atatcccgat gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat
    63241 tatttcctct gagaaaatgc aaacctagtc agacgaagga gccttgtctt tactatcatt
    63301 tgggacaatg tctgtgtcct tattatttcg acgtagatcc gaaagtctat acgaaaatcg
    63361 ttgatgaagt gaaaaagttt ttaaacggag gacacgaaaa aatcgagtca gagatccatc
    63421 agaaaatgga aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa taccgagacc
    63481 agatccgtgc cattgagaca atcatgactc gccaaaaaat gacaaatact gatttactgg
    63541 atcgagatgt atttggttat gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg
    63601 taagacaagg aaagctaatt gaacgagatg tttcaatgtt tcctttctat aacgaagcag
    63661 aagaagattt cttgacttac ctcggtcagt tttatcagga aaatgagcat ttcattccta
    63721 aagaagtctt gattcctgat acgatcgaca agcccagtgt agaagctttg ttagctacga
    63781 aagtgctgca accaaaacga ggagaaaaga aaaaattagt tgaattggcg aataagaatg
    63841 cctcagttgc tttgaatgaa cggtttgatc tgattgccag aaaacaggaa cggacgatcg
    63901 gagcagttga acgtttagga aacgcgatga atatcccgac cccaatacgt atcgagtctt
    63961 ttgataactc taatatcatg gggacgaatc ctgtggctgc gatggtcgta ttcattgatg
    64021 gaaaaccggc aagaaaagaa tatcggaaat ataaaataaa aacagtcaac ggaccggatg
    64081 attatgcatc aatgagagaa gtcatctatc gcagatattc acgagtgatt cgcgaagggt
    64141 tgcctttacc tgatttgatc ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag
    64201 atgtcttaga aaatcaatta ggaatcgata ttcctattgc tggcatggca aaaaacgata
    64261 aacataaaac aaatgagttg ctattcggta tagatttggc tgtagtaccg cttgagcgaa
    64321 attcaccaga gtttttctta ctccagcgaa tccaagatga agtccatcga ttcgcaatca
    64381 ctttccatcg gcagctacgc agtaaaaaca gttttgcttc ccgattagat gaaattgaaa
    64441 ggttaggacc aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca
    64501 cagcagccac gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg
    64561 tatttgataa attacatcag gaatgaggaa agggcacttt acaaagaaag taaagtgtcc
    64621 tatcatttta tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa taaatcgctt
    64681 acgtctggtg tctaaggata tatgtgattt cttgaagaag gagtgaagaa atgttagaag
    64741 tcaaggatct tgttaaaact tttgggaact acacagctgt cgatcatgtc tctttccaaa
    64801 tccccgatgg aaaaatcatg gggctaattg gccaaaacgg ggctgggaag acaacgactt
    64861 ttcgtttgat cttggatttt ttgacacaag accaaggaga agtgttatgg aatggccatc
    64921 atcttgggga gaaagattat gatgtcatcg gttatcttcc ggaggaacgg ggactttatc
    64981 cgaaagtaac gattcaagac caactgcttt attttgcaga acttagaggg aaatcaagaa
    65041 aagagattga accaaaaatc gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga
    65101 cggataaagt aaaatctttg tctaaaggaa atcagcaaaa agtccagctg atcgccacgt
    65161 taatccatga acctaaattg atcattttgg atgagccatt cagcgggtta gatccggtaa
    65221 atgctgaatt gctaaaagat ggaatattgg aattgaaaaa acatggttca tgtgtcattt
    65281 tttctagcca caatatggat aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa
    65341 acggaaaaat ggttttgaat ggaaaagtac atgagattcg tgaatcattt ggccgtacga
    65401 agttattctt ggaatctggt ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa
    65461 aagtgatcct tcatgaagac aatacgttag agattacctt agaagatcct gaagctggga
    65521 aagaaatctt tactcgtgcg actcagttcg gctacattcc tatgtttaac cagcaaccgc
    65581 caactttgga agagattttc aaattgaaag caggtgaaat cgatgcgtaa gttttggatt
    65641 attaccaaag atgtctattt aaaaaatgtt aagtcgattt ctttcttgat catgatatta
    65701 gtccctttta tcatgctggg tatcatttat gtagcaggaa actttgctca acaaaatagt
    65761 gatgtcaata aaatcggcgt attggctgat aatgagcaaa tcagtcagca aatgagccaa
    65821 gtcaaaacag atgattttga gttcaaagca gttgattctg aagaaaaagc acaaaaagag
    65881 ctttcggaag aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga taagataaat
    65941 gggatgcttt atagcgaaaa ttcattagga caaagtacac aactactgat acaacaacaa
    66001 ctgactggct tgcaatcgat gcttcgagca agtcaattag gcatttcacc agaagaagta
    66061 gctagcttga gccaacaagc aagtttgact cgacaaaaag tcaattttga ttcaaaagga
    66121 aaaatgacag ttggagaaga taactcgaca gtccaatatg tcgtaagtta tgtagcaacg
    66181 attgtcttgt tcattattat tttgacgtat gcgcagatca ttgcacaaga gatcgcttct
    66241 gaaaaaggca cgcggatcat ggaagtgatc ctgtccagta cgactgcaca aaagcatttt
    66301 tacggaaaac taacaggtgt tttacttgtt gcattaacgc aaatggcatt atacggtatt
    66361 atttttggta taggatacag tcaattcaaa gatatggaaa tggtcaaaaa tatattagac
    66421 ggtatctcat tggataactt gattggacca ttcttgtggt attctcttct attcatgatc
    66481 tttggcattt tgattttttc tgttttagca gctttatgtg gctctttggt caacaaagca
    66541 gaagatacag caaaagcaat tttgccagtg acttatctat ctctaggcgg atatatgctt
    66601 ggattgatac taggagcttc agatccaaac aatatcgtta ttcggatcac ttcgtatatt
    66661 ccattcttat cttcatatat tatgccggtt cgcttagcaa atgagacagt cggtatgggt
    66721 ggagcattga tttccttgtt ggtacttgta gccgtaacat tcttattgat gatcttgtct
    66781 gcaaacatgt acaaatcgaa tgtactcgtg tatagcgaag gcggattatg gagttcattg
    66841 aaacaatcga tttcaattat gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat
    66901 agaaggaagc tggacattta ttgttcccag cttctttttt gtatagacct atttctattt
    66961 ttttattata ttggatatat atattcattg aaaatagagc ataacaactt tataatgttg
    67021 agtataccaa tttttggagg cgttttaatg gaccgctatt tagatttact ttcagagaag
    67081 ttccctacaa cggaagatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    67141 ccaaaaggaa cagagttatt tttgagcgat atacatggcg aatttcctgc ttttgatcat
    67201 attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt ctctgaacaa
    67261 atgacggaaa cggagatcag ccaattcact atttttacgg cttatccaga atatgtgttg
    67321 acgactgatt ggtacaaaaa acaagataaa tcccagctga tccatcgttt gatcgatctt
    67381 ttacggttta cgacagtcaa atacactcga tcaaaagttc gaaaaggatt gccgaaagaa
    67441 tatagctata tcattgagga actcttgtat atcgatgatc gtatcaacgg gaaaaaagac
    67501 tatgtaaaaa aaatcattga acaacttgtc ttgatgaagg aagaagaaaa atttttaaca
    67561 aaacttgcgc agacgatcca aaaatctgtc atcgatcatt tgcatatcgt gggtgatatt
    67621 tttgatcgag gaactcaagc agccaaagta atggatcgaa tcatggattt ttcctctgta
    67681 gatatccagt gggggaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    67741 tgtttattga cgcttttacg gattgccgca cgctacaact atttatttga attagagaaa
    67801 gaatatggat taaatttacg tccattattt tcttttgccc atcaaaccta tcagaagaat
    67861 ccagtattta cccctaaaaa caggaaagat ctatctgaaa gagagcaaga agaattagaa
    67921 aagatccatc aagcactttc tatcctccaa ttcaaatcag aacatcagtt gttgaatcga
    67981 cgacctgaat ttaaaatgga cgatcgtaaa ttgttggaaa aaatcaacta tgaggaatca
    68041 acgatcgttt tagaaggtca gctctatgag ttgaaaggga cttgttttca aacgatccag
    68101 ccagatgatc ctaaaaaatt cttggaagag gaacaaaaag tgatcgatag tttgatgtat
    68161 tcattccagc actcgttaag aatgcaaaaa cacatgacgt ttttgatcga aaaaggggga
    68221 atgtatctca cgaacaatca gcatgtactg ttccatggct gtatacctgt agatgaaaag
    68281 gggcagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggattt
    68341 tttgaaaaac agataactga aagcgcaaaa gatttgactg caaatgaaga tttggcgacc
    68401 gatttgattt ggtatgcgtg gagcgggcca ttttcgcctt tattcggcaa gagtaaaatg
    68461 gcaacttttg agcgctattt tctcacagag cctcatacgc acgtcgaaaa aagcaatccc
    68521 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttggcaga attcgatgca
    68581 aaagaagaag gttcagcaat catcaatgga cacacgccag taaaagtaaa aaaaggagag
    68641 tcaccgatca aagcaaacgg aaaagcattt gttattgacg gaggtctatc caaagcttat
    68701 caaaaaacaa ccgggattgc tggctactca ttactatgca attcttacgg tttccaaatc
    68761 gtgacacatt atccgttctt gcctattgaa caacaatttg caaaaaaatc tgatcaaacg
    68821 aatgtaaaaa aagtgatcga gcagcagctc ccacgcaaac ttaatcggaa tacgacaaaa
    68881 ggaatggaat tacagcaaca gattactcaa ctccaaaggc tgttggatta tcgaaaggac
    68941 gattgagaaa aatgctagcc aacattgctc aatagactaa aaagcgactg caacaaaagg
    69001 cgtgtcgcag tctccttcgg aattagctca aaaaacggta aaatattaga agctattttt
    69061 ctgtttattt ctctaatact caggtcaggg cgctgatttc acgacctctt ccttaaacgg
    69121 tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aacaaacatg agaagctgtt
    69181 ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct aatcagaaaa
    69241 acgattaaaa ttcattcaca aagaaatcga ataaacgctg ttcagatgaa tctacacgat
    69301 gagaaagttc cggatgccat tggacaccaa ggattttgtg catattatct ttactttgga
    69361 ctgcttcaat gagtccatcg ttggaaaagg cgatggcttt taacaaagga gaaagatctt
    69421 tgactgcctg atgatgatag gaattcacaa agtatgaatc agggagaagg tcacttaata
    69481 aactgtccga aacgattttg acttcgtgag ttgcaaactg cggggcagtg ggctgttgtt
    69541 cgtgttttac ggaccatttt ggatataatg aaagatcttg ataaagtgtt ccctccaaca
    69601 cgacattcaa tagttgaagt cctcggcaga ctgcaaaaat cggttttctt tgtttcaatg
    69661 cttccaagat caatgcctgt tcgaattggt ctcgttgaat atttgtttcc gtcagttttg
    69721 gatgagggtc ttccatgtaa agttgcggac taacatcctg accgcctgca agaatgagtt
    69781 tgtcgatttt ctcaattagt tgaggcgctg aatctggaga catgactggc agaataagag
    69841 gtaatccttg agcatctaaa actgcatcca caaatccctg cggtgtatag cttacttgat
    69901 ttccttggaa agtatctgtc gctcgaatca gttgattccc ggcaatgccg atgattggtt
    69961 tcatttttat tccccctaaa gtagactaat tgtaacaagg atttaaaaaa atttaaacta
    70021 tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaattt
    70081 tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa
    70141 gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg
    70201 acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg
    70261 aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc
    70321 tttggaacaa atccgaaaag cagaagaaga aaatgagaag cgtgctcgaa ccctacggca
    70381 gcagttgaag acctatgcag aagaaaaaga gcaagaattg gagataatcc aaacgaacag
    70441 tcggcaacag ctgaaacagt tggtagaaga aaaagaacaa ttcgaaaagc aagaattaga
    70501 aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagacca
    70561 atatcaaaag catcagagtc aagcaatcgg tgcaatcatt gaaagggtga aagaaactta
    70621 tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga
    70681 ttttacaagc agtacaagga atccaaaatg ttgaaatccg ggatctgtat caaaatagaa
    70741 caaacaacca gtgggtagaa aaatattttc cagataccac gattattgat aaagaagcaa
    70801 aattaagcgc tttaggcaat cggctgaaag acatccaaga agctatccaa ttcatacaac
    70861 accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag
    70921 acttggaagc aaattattcc gaagaaaatt tcatgaataa attagaagaa gtccttgatt
    70981 taaaagaaaa gtgggaacag ctaactgaaa aacgtgatcg tttagaagaa gaggaagaat
    71041 ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca
    71101 tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactaagta
    71161 agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt
    71221 atgttgcttt aaaaaaatca gttgctgcta tagaagaaat tgctgggaaa tacgggttca
    71281 ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa attgcaagaa accaaacagc
    71341 aattatccat tgttcacgag cagcaaaaaa agataacgac agcgttaggt cagtgcagcg
    71401 gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgggaaa
    71461 aagtaaaaga acggtttatc catgccactt atctgatcgt tttgcaaggt tgggtagatg
    71521 cagaagaaaa acagaatttg atgcatgtgt taaacgaaac tgtatcagaa gaagatatat
    71581 acgttagttt cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga
    71641 aaaaccatcc gcttatcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat
    71701 atgaagaagt agatccaacg ccttggatga caccatttta tcttgtcttc ttcgggatga
    71761 tggtcgcaga tgttggttac ggattgctga tgctcattgg gacaatacta gctcaaaaac
    71821 tatttgtttt accccgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcggtac
    71881 catcgatcat ctggggattg atctacagtt catttttcgg acaggcatta ccaaaagaac
    71941 tatttggtat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt
    72001 tgattttatc cgtgattttc ggtctgatac agatcttggt tggattattc gtggcagcaa
    72061 aggaaaatat caggaggaaa gattacttga gtgctgtcag tgatggtttt gcctggcaag
    72121 ggatcctgat tgggatcgtt attgcattga ttggtgtcat gttgataaaa aatacagcat
    72181 ttgtttatct tggcgcgggg ctatcgatca ttgctgcttt atgtatctta atcgtgccga
    72241 ttatccaatc gccgtctaaa gtcaagggtg ctgccaaagg agcgtataat ctttacggat
    72301 tgactggtta tatcggtgat ttagtcagtt atacacgttt gatggcacta ggtatttcag
    72361 gcggaagtat tggcgcggca ttcaatatgc tagttgcttt catgccgcca gccgctcgat
    72421 tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgt
    72481 taagtgccta tgtccatggt gcacgattgc agtatgtcga attttttgga aaattttata
    72541 caggaggcgg acgtgccttt gacccgctga aaacggcgga aaaatacgtc aacatcaatc
    72601 ataagaaaaa aaacaataaa taattggagg atttttatac aatgatggat tacttaatta
    72661 gtcaaaatgg tggaatggta tttgcagtat tagcgatggc aacagcgacg attttttcag
    72721 ggattggttc agctaaaggt gttggaatga caggtgaggc agcagcagcg ttaacaacaa
    72781 gtcaacccga aaaattcgga caggctttga ttctgcaatt gcttccaggt acgcaagggt
    72841 tgtatggatt cgttatcgcc tttttgattt ttatcaactt aggaaatgat atgtcagttg
    72901 tacaaggatt gaactttcta ggcgcttctc tgccgattgc ttttacaggt ttgttttcag
    72961 gtatcgccca agggaaagtt gcagcagcag gtatccaaat tttagcgaaa aaacctgaac
    73021 atgctacaaa aggaatcatt tttgctgcga tggttgaaac atatgcgatt ttaggtttcg
    73081 ttatttcctt cctattagta ttgaatgcct aagcataaag gaggaaaagc agatgaacgc
    73141 cattgaaaaa atcatctctc agatgaatga agcagcagag caagaaagag cggcgttaga
    73201 gcaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aaatcgaaac
    73261 agaacatcaa aaacaaaaag aaaaacaaat cgaattactt gaaaaaaaat accgtcagct
    73321 taggaatcgc caacaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt
    73381 acgacgattg tttgcagatg caatagctga aatggagaat tgggatgaat ctgaacaaat
    73441 ccagtttatc aaaaattcat tgtattcatt acctttgaca ggcaaagtaa cattcattgc
    73501 tggagaaaaa tcagcagctt atctttctca gacattgctg gatgaatgga acaatgaact
    73561 tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa
    73621 cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac
    73681 gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac
    73741 cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt
    73801 gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt
    73861 tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag
    73921 cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc
    73981 tatacgatgc gctatacttt ccataatctg aaggttttga caaaagccga gatgacaaat
    74041 caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat
    74101 gccattcata cgcaagtttc aaatgaactc cctgaaagca ttatggacag tatacgagaa
    74161 gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga
    74221 cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca
    74281 gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag
    74341 aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa
    74401 gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct attagagaca
    74461 gattatgctg attttctgcg ccctgtcatc cgtgagggaa atcttgatct cgtaaagctt
    74521 gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc
    74581 ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt
    74641 ttactggtta tcggcaaaag aagccatttc gaaccagaaa caatcagaaa gcgggtgaga
    74701 caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa
    74761 attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga
    74821 agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag
    74881 cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa
    74941 tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt
    75001 aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat
    75061 aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat
    75121 gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc
    75181 gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac
    75241 tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat
    75301 ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt
    75361 tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg
    75421 agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca
    75481 taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt
    75541 cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat
    75601 gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc
    75661 acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc
    75721 agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa
    75781 atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac
    75841 ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga
    75901 acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat
    75961 ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat
    76021 ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga
    76081 aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga
    76141 acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat
    76201 cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg
    76261 ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc
    76321 gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca
    76381 tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga
    76441 acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg
    76501 tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga
    76561 tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac
    76621 ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga
    76681 cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt
    76741 agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga
    76801 aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga
    76861 tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa
    76921 atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa
    76981 tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc
    77041 ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac
    77101 cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa
    77161 tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg
    77221 atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc
    77281 ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg
    77341 atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg
    77401 aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg
    77461 accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg
    77521 cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg
    77581 cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt
    77641 atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag
    77701 gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc
    77761 ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga
    77821 gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa
    77881 ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct
    77941 atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg
    78001 atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag
    78061 gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga
    78121 tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag
    78181 aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc
    78241 gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag
    78301 atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa
    78361 tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg
    78421 aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag
    78481 acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga
    78541 cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg
    78601 atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc
    78661 aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga
    78721 cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc
    78781 gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat
    78841 ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc
    78901 ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact
    78961 taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac
    79021 tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa
    79081 cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat
    79141 ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat
    79201 attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat
    79261 cctaaaattg gcctttgtta tccaattgat tggtacggtt gctttatctt ttgtatttgt
    79321 gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt
    79381 ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta
    79441 tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg
    79501 tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac
    79561 cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg
    79621 ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt
    79681 gacacctaga acggctggat attattcagt tgattacctc catatgagcc atgcaggtct
    79741 gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt
    79801 gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac
    79861 gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt
    79921 attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac
    79981 gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac
    80041 agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat
    80101 agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa
    80161 agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt
    80221 tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg
    80281 cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg
    80341 aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat tcctttattt
    80401 atcaataggt tataattcat atttttttat tttttctttt acgatactta gtccagaagc
    80461 taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg gttgcgcacg
    80521 tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt cattctcaat
    80581 tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga cacgattcgt
    80641 tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg taagacttaa
    80701 tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga tgacttccac
    80761 atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct tgctttggac
    80821 atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt ttgtcattcc
    80881 agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga tcgtatgttg
    80941 atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac cttctttttc
    81001 aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta taggattatt
    81061 acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct gaatcaatat
    81121 atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat gatataactg
    81181 agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat cacctggaga
    81241 gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa tagatagaga
    81301 ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag tcaattgaaa
    81361 atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt tattactagt
    81421 tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa ccagaccaaa
    81481 atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa agacattggg
    81541 agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa ttccatattt
    81601 ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct ctctttggga
    81661 gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta attgttgaat
    81721 caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta
    81781 attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac catacaattt
    81841 taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat tgtctatgaa
    81901 aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga ttcaagtaat
    81961 gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc gattggtgga
    82021 aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt atttggggga
    82081 gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc agtatcattt
    82141 accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac ccaaagcatt
    82201 gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc tggcaacaga
    82261 ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc aacaagcatt
    82321 gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg tttacgatat
    82381 tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc ctaaaataac
    82441 gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca tttgtacgtt
    82501 gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg cacatatcaa
    82561 tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga tggaaacttc
    82621 tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc tgcttatagc
    82681 catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg aattagtttc
    82741 tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg cagttggtct
    82801 agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga aaggggcttt
    82861 aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt caaaggaaat
    82921 ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca aaaaattgcc
    82981 attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga atttgagctt
    83041 agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt agcaacagca
    83101 tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc tactcaaagc
    83161 ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc tagtattatg
    83221 ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa gattaaagaa
    83281 gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt ggctaaattg
    83341 cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat cgtgacagca
    83401 gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac aatcaatgaa
    83461 gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga aagaatgaat
    83521 aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga atgtgaatta
    83581 aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg tacctcgttt
    83641 atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac cttatagcta
    83701 caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt
    83761 ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata ctaactatcc
    83821 tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt
    83881 gccaggtaat ccttttaagt cagtaatctc aagaccggca gctttatgtt gctcgtgtaa
    83941 tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta gatcatacgc
    84001 accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa gtgtcaaata
    84061 aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc
    84121 cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg tgtgagccat
    84181 tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga agtaaggaaa
    84241 tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga aaataaaact
    84301 gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc aatttgctga
    84361 ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga ttgcagtaat
    84421 cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag aagagacgaa
    84481 agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag aaatttcttc
    84541 ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt attattataa
    84601 tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac
    84661 caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac gtggtagcca
    84721 ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc
    84781 agctttctct cagttttttt atttattaca gtacatattt tttgattgct tcagctacac
    84841 cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga gaaacggcat
    84901 ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc tcgttatctc
    84961 cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc gcaactgccg
    85021 cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt ttgaccgttg
    85081 tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt
    85141 cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca ggtgttcgat
    85201 attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg atatctcgat
    85261 taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca cggcgtgcca
    85321 tcacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg attttacctg
    85381 atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta tcttgaaaaa
    85441 ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg cataaaacga
    85501 tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt gttacttctc
    85561 ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata gacataattt
    85621 cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg ctggcaaatt
    85681 cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca accatttctt
    85741 ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca acaagctgac
    85801 taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat
    85861 gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa ttcacagcag
    85921 tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct tggactaatt
    85981 ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc aaaaaacgtt
    86041 tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag taagtgccca
    86101 agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca agaaatggca
    86161 gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc
    86221 gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg aagtacactt
    86281 gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg ccactcatgg
    86341 aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta agatcaaatg
    86401 ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt cgatctgcat
    86461 cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt tggatcacac
    86521 tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac acttctgttt
    86581 ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag atatgttcaa
    86641 atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat aaagtcacca
    86701 gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa
    86761 agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta aaacggctat
    86821 gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt
    86881 ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg atatagttat
    86941 ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa ggggttgtca
    87001 taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt gttccattgc
    87061 tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact tgcagaatac
    87121 agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag gggttggtca
    87181 agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc atttgtacca
    87241 aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccgcg tttttcctga
    87301 ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg acgctgagcg
    87361 ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca ataagaatgg
    87421 gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa taggagcatt
    87481 tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa aatcaaagaa
    87541 atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct gatcactgga
    87601 gcgtaacctg catctttcag tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc
    87661 ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt
    87721 tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc gtttttttga
    87781 taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt gtataaattc
    87841 atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa aggttttctg
    87901 taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta gctatgagaa
    87961 gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc aaattgcgta
    88021 caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa gggcaaatgg
    88081 tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag acgggcgatg
    88141 agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc cgtgcaggag
    88201 aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc tatcgaacac
    88261 catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt ttgaaagacc
    88321 gtcccacagg agggaaggtt cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg
    88381 gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt ttgatttttt
    88441 gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga ttggaaatta
    88501 gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca cacgcgatct
    88561 tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc caaaactatc
    88621 ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact gacttgaata
    88681 ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca tggtatgcta
    88741 tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa gatactccac
    88801 gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt
    88861 acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat gatctgtttg
    88921 atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg caagaaatcg
    88981 gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa gaagatttaa
    89041 gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt tctgactctg
    89101 aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca gcctatcaga
    89161 ataaggaggt gtgacac
//
LOCUS       DANJLF010000004        84918 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000004 DANJLF010000000
VERSION     DANJLF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..1589)
                     /locus_tag="PWT25_000310"
     CDS             complement(<1..1589)
                     /locus_tag="PWT25_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1020422.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
     gene            complement(1604..5125)
                     /gene="mfd"
                     /locus_tag="PWT25_000311"
     CDS             complement(1604..5125)
                     /gene="mfd"
                     /locus_tag="PWT25_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL1020423.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(5142..5702)
                     /locus_tag="PWT25_000312"
     CDS             complement(5142..5702)
                     /locus_tag="PWT25_000312"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL1020424.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            6055..7029
                     /locus_tag="PWT25_000313"
     CDS             6055..7029
                     /locus_tag="PWT25_000313"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL1020425.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            7199..8599
                     /locus_tag="PWT25_000314"
     CDS             7199..8599
                     /locus_tag="PWT25_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL1020426.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(8677..9399)
                     /locus_tag="PWT25_000315"
     CDS             complement(8677..9399)
                     /locus_tag="PWT25_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1020427.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(9478..9984)
                     /locus_tag="PWT25_000316"
     CDS             complement(9478..9984)
                     /locus_tag="PWT25_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL1020428.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            10107..11906
                     /locus_tag="PWT25_000317"
     CDS             10107..11906
                     /locus_tag="PWT25_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL1020429.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(11974..13548)
                     /locus_tag="PWT25_000318"
     CDS             complement(11974..13548)
                     /locus_tag="PWT25_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1020430.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(13803..14312)
                     /gene="mreD"
                     /locus_tag="PWT25_000319"
     CDS             complement(13803..14312)
                     /gene="mreD"
                     /locus_tag="PWT25_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL1020431.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(14316..15170)
                     /gene="mreC"
                     /locus_tag="PWT25_000320"
     CDS             complement(14316..15170)
                     /gene="mreC"
                     /locus_tag="PWT25_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL1020432.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(15325..15963)
                     /locus_tag="PWT25_000321"
     CDS             complement(15325..15963)
                     /locus_tag="PWT25_000321"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL1020433.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(15964..16614)
                     /locus_tag="PWT25_000322"
     CDS             complement(15964..16614)
                     /locus_tag="PWT25_000322"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL1020434.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(16627..17526)
                     /gene="rsgA"
                     /locus_tag="PWT25_000323"
     CDS             complement(16627..17526)
                     /gene="rsgA"
                     /locus_tag="PWT25_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL1020435.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(17691..19760)
                     /gene="pknB"
                     /locus_tag="PWT25_000324"
     CDS             complement(17691..19760)
                     /gene="pknB"
                     /locus_tag="PWT25_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL1020436.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(19757..20497)
                     /locus_tag="PWT25_000325"
     CDS             complement(19757..20497)
                     /locus_tag="PWT25_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL1020437.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(20510..21868)
                     /gene="rsmB"
                     /locus_tag="PWT25_000326"
     CDS             complement(20510..21868)
                     /gene="rsmB"
                     /locus_tag="PWT25_000326"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL1020438.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(21868..22806)
                     /locus_tag="PWT25_000327"
     CDS             complement(21868..22806)
                     /locus_tag="PWT25_000327"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL1020439.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(22799..23290)
                     /gene="def"
                     /locus_tag="PWT25_000328"
     CDS             complement(22799..23290)
                     /gene="def"
                     /locus_tag="PWT25_000328"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL1020440.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(23305..25713)
                     /gene="priA"
                     /locus_tag="PWT25_000329"
     CDS             complement(23305..25713)
                     /gene="priA"
                     /locus_tag="PWT25_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL1020441.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(25966..26271)
                     /locus_tag="PWT25_000330"
     CDS             complement(25966..26271)
                     /locus_tag="PWT25_000330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL1020442.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(26276..26890)
                     /gene="gmk"
                     /locus_tag="PWT25_000331"
     CDS             complement(26276..26890)
                     /gene="gmk"
                     /locus_tag="PWT25_000331"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL1020443.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(27377..27661)
                     /locus_tag="PWT25_000332"
     CDS             complement(27377..27661)
                     /locus_tag="PWT25_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020444.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(27679..28569)
                     /locus_tag="PWT25_000333"
     CDS             complement(27679..28569)
                     /locus_tag="PWT25_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL1020445.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(28589..28807)
                     /locus_tag="PWT25_000334"
     CDS             complement(28589..28807)
                     /locus_tag="PWT25_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL1020446.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(28777..30477)
                     /locus_tag="PWT25_000335"
     CDS             complement(28777..30477)
                     /locus_tag="PWT25_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL1020447.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            30744..31517
                     /locus_tag="PWT25_000336"
     CDS             30744..31517
                     /locus_tag="PWT25_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020448.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(31598..32167)
                     /gene="thiT"
                     /locus_tag="PWT25_000337"
     CDS             complement(31598..32167)
                     /gene="thiT"
                     /locus_tag="PWT25_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL1020449.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(32233..32335)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(32341..33312)
                     /locus_tag="PWT25_000338"
     CDS             complement(32341..33312)
                     /locus_tag="PWT25_000338"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL1020450.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(33406..34779)
                     /gene="glmU"
                     /locus_tag="PWT25_000339"
     CDS             complement(33406..34779)
                     /gene="glmU"
                     /locus_tag="PWT25_000339"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL1020451.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(35282..36100)
                     /gene="purR"
                     /locus_tag="PWT25_000340"
     CDS             complement(35282..36100)
                     /gene="purR"
                     /locus_tag="PWT25_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL1020452.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(36267..37091)
                     /locus_tag="PWT25_000341"
     CDS             complement(36267..37091)
                     /locus_tag="PWT25_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1020453.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(37064..37744)
                     /locus_tag="PWT25_000342"
     CDS             complement(37064..37744)
                     /locus_tag="PWT25_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL1020454.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(37767..38705)
                     /locus_tag="PWT25_000343"
     CDS             complement(37767..38705)
                     /locus_tag="PWT25_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL1020455.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(38950..39813)
                     /locus_tag="PWT25_000344"
     CDS             complement(38950..39813)
                     /locus_tag="PWT25_000344"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL1020456.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(39955..42528)
                     /gene="mprF"
                     /locus_tag="PWT25_000345"
     CDS             complement(39955..42528)
                     /gene="mprF"
                     /locus_tag="PWT25_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL1020457.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            42669..43475
                     /gene="proC"
                     /locus_tag="PWT25_000346"
     CDS             42669..43475
                     /gene="proC"
                     /locus_tag="PWT25_000346"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL1020458.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(43554..45110)
                     /gene="rny"
                     /locus_tag="PWT25_000347"
     CDS             complement(43554..45110)
                     /gene="rny"
                     /locus_tag="PWT25_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL1020459.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(45521..46570)
                     /gene="recA"
                     /locus_tag="PWT25_000348"
     CDS             complement(45521..46570)
                     /gene="recA"
                     /locus_tag="PWT25_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL1020460.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(46679..47920)
                     /locus_tag="PWT25_000349"
     CDS             complement(46679..47920)
                     /locus_tag="PWT25_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL1020461.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(48160..48738)
                     /gene="pgsA"
                     /locus_tag="PWT25_000350"
     CDS             complement(48160..48738)
                     /gene="pgsA"
                     /locus_tag="PWT25_000350"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL1020462.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(48947..49870)
                     /locus_tag="PWT25_000351"
     CDS             complement(48947..49870)
                     /locus_tag="PWT25_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020463.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(50609..51895)
                     /locus_tag="PWT25_000352"
     CDS             complement(50609..51895)
                     /locus_tag="PWT25_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL1020464.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(51888..53156)
                     /locus_tag="PWT25_000353"
     CDS             complement(51888..53156)
                     /locus_tag="PWT25_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL1020465.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            53408..53869
                     /gene="mscL"
                     /locus_tag="PWT25_000354"
     CDS             53408..53869
                     /gene="mscL"
                     /locus_tag="PWT25_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL1020466.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(53929..54375)
                     /locus_tag="PWT25_000355"
     CDS             complement(53929..54375)
                     /locus_tag="PWT25_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1020467.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(54468..54713)
                     /locus_tag="PWT25_000356"
     CDS             complement(54468..54713)
                     /locus_tag="PWT25_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL1020468.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(54828..55406)
                     /locus_tag="PWT25_000357"
     CDS             complement(54828..55406)
                     /locus_tag="PWT25_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1020469.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(55483..56013)
                     /locus_tag="PWT25_000358"
     CDS             complement(55483..56013)
                     /locus_tag="PWT25_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL1020470.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(56017..56877)
                     /gene="rlmB"
                     /locus_tag="PWT25_000359"
     CDS             complement(56017..56877)
                     /gene="rlmB"
                     /locus_tag="PWT25_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL1020471.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(56870..57265)
                     /locus_tag="PWT25_000360"
     CDS             complement(56870..57265)
                     /locus_tag="PWT25_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL1020472.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(57262..58674)
                     /locus_tag="PWT25_000361"
     CDS             complement(57262..58674)
                     /locus_tag="PWT25_000361"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL1020473.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(58671..59207)
                     /gene="cysE"
                     /locus_tag="PWT25_000362"
     CDS             complement(58671..59207)
                     /gene="cysE"
                     /locus_tag="PWT25_000362"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL1020474.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(59293..59532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(59529..60989)
                     /locus_tag="PWT25_000363"
     CDS             complement(59529..60989)
                     /locus_tag="PWT25_000363"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL1020475.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(61159..62316)
                     /locus_tag="PWT25_000364"
     CDS             complement(61159..62316)
                     /locus_tag="PWT25_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL1020476.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(62350..63720)
                     /gene="radA"
                     /locus_tag="PWT25_000365"
     CDS             complement(62350..63720)
                     /gene="radA"
                     /locus_tag="PWT25_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL1020477.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(63783..64310)
                     /locus_tag="PWT25_000366"
     CDS             complement(63783..64310)
                     /locus_tag="PWT25_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL1020478.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            64540..65346
                     /gene="proB"
                     /locus_tag="PWT25_000367"
     CDS             64540..65346
                     /gene="proB"
                     /locus_tag="PWT25_000367"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL1020479.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            65343..66590
                     /locus_tag="PWT25_000368"
     CDS             65343..66590
                     /locus_tag="PWT25_000368"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL1020480.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            66666..67010
                     /locus_tag="PWT25_000369"
     CDS             66666..67010
                     /locus_tag="PWT25_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020481.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(67127..67453)
                     /locus_tag="PWT25_000370"
     CDS             complement(67127..67453)
                     /locus_tag="PWT25_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020482.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(68068..69270)
                     /locus_tag="PWT25_000371"
     CDS             complement(68068..69270)
                     /locus_tag="PWT25_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1020483.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            69387..69902
                     /locus_tag="PWT25_000372"
     CDS             69387..69902
                     /locus_tag="PWT25_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1020484.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(70101..70547)
                     /locus_tag="PWT25_000373"
     CDS             complement(70101..70547)
                     /locus_tag="PWT25_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL1020485.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(70594..72072)
                     /gene="msr(C)"
                     /locus_tag="PWT25_000374"
     CDS             complement(70594..72072)
                     /gene="msr(C)"
                     /locus_tag="PWT25_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL1020486.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(72426..73055)
                     /locus_tag="PWT25_000375"
     CDS             complement(72426..73055)
                     /locus_tag="PWT25_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1020487.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(73039..73503)
                     /locus_tag="PWT25_000376"
     CDS             complement(73039..73503)
                     /locus_tag="PWT25_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1020488.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(73990..75258)
                     /locus_tag="PWT25_000377"
     CDS             complement(73990..75258)
                     /locus_tag="PWT25_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL1020489.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(75489..76226)
                     /locus_tag="PWT25_000378"
     CDS             complement(75489..76226)
                     /locus_tag="PWT25_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1020490.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(76293..76862)
                     /locus_tag="PWT25_000379"
     CDS             complement(76293..76862)
                     /locus_tag="PWT25_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL1020491.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            76990..77862
                     /locus_tag="PWT25_000380"
     CDS             76990..77862
                     /locus_tag="PWT25_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1020492.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            78204..79076
                     /locus_tag="PWT25_000381"
     CDS             78204..79076
                     /locus_tag="PWT25_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1020493.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            79073..81031
                     /locus_tag="PWT25_000382"
     CDS             79073..81031
                     /locus_tag="PWT25_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1020494.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(81363..82214)
                     /locus_tag="PWT25_000383"
     CDS             complement(81363..82214)
                     /locus_tag="PWT25_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1020495.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            82337..82804
                     /locus_tag="PWT25_000384"
     CDS             82337..82804
                     /locus_tag="PWT25_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL1020496.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(83067..83549)
                     /locus_tag="PWT25_000385"
     CDS             complement(83067..83549)
                     /locus_tag="PWT25_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL1020497.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(83649..84512)
                     /locus_tag="PWT25_000386"
     CDS             complement(83649..84512)
                     /locus_tag="PWT25_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL1020498.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        23985 a        18210 c        15313 g        27410 t
ORIGIN      
        1 ttaatttttt tatttaatcg caggattttc ttaccaaaag gcaacattag ccattctcgt
       61 actgtaaaca gacgaagacg aatcactgca taaatgaaca cagaagcccc gatcccaact
      121 cctatcacgc tgacaaaaaa agcagtggaa cgatgagtta caggaccaaa tactatattc
      181 aatacaccat aatacagcag cagtactact gtcatacaaa gaaggctgaa catcaacttc
      241 cgtccaaaat gccgttcttt ccaaaaagca tttagctctt ttgtttcaat tcgaacaaaa
      301 taccaaagcg tcacacctaa tcctaaaagg gtagacaggc ttgcacctgc tgttcctaat
      361 aaaccggtca gaaaagaagt agtcaatcct tttaccaata gtccccatcc tgcgcctttt
      421 aaagaagggc gaaacgagtt cttgctttga gcaatacttt gataagcttg gatgactgca
      481 gtgaaagcaa tggaaaaaac aaataataca agcgctgcat ttcccgcata atctttaaac
      541 aacgcataat tgatataagg gagcagcaag gctaatccca aagacgcagc taatgctaaa
      601 gcggtcgtca atctaagata gattttggct gtctgtaaaa actgacgata gatccggttg
      661 gtcaaatgac gagtcaacgc aggcaggaat gtcgcactta atgcagtggc aatcactaaa
      721 cctaattgta ctaaaggctg acctcgatca tagacccctt ttgctatctt cgcaccgtat
      781 tctgacagtc cgtaaacttc caaagcattt tttacaaaga aagagtcgat cagctggaaa
      841 aagataagca atccactata aatcgaaacc agtccgcctt ctaccagaaa acgccggatc
      901 aagggctttg acggctgttt gaacaatgga aaccgtcgga agcttaacgc tcctccatga
      961 atcttttgtt catagtagtg aaggatccca tatgcgcata aaccgccaaa aacagctcca
     1021 ctcattgctg ccgtccctgt ttggtacacc gtccatccaa gctttctaaa agcaaacgca
     1081 ctacagacaa tcacaaaaac acgaacgaac tgctcaacta cttgagaaac agcagttggt
     1141 tccatcaaga atcttccttg gaaattccct cgataaaaag atagaccagg catcagcaaa
     1201 aaggtaaagg aaactacttg gatcagcggt ttcaattgta tgtctcccat catccaagca
     1261 atccagctgc taaatgcaaa gacaaacaac cacaaaaaga cacctgtcca aaatactaag
     1321 ggataaagat tttgcaaagc ttgctttcgc tcgaaaggat tctttctttc tgcaacgtac
     1381 tttgatatga actgcggaag accggataaa gctaaagtca ttgctatccc ataaattgga
     1441 taaacttgct gataaacata aaaaccttcg tcgccgacta agttttggag gggaattcgg
     1501 taaagggcac ttaatacttt tgctatgaaa gaagcaatcg tcaatacaaa tgctccttgc
     1561 atgacgcgcc gcatttcttt atgatccaaa tcattgctcc ttcctacgct gacagatatt
     1621 tttgctcacg caacgctttg acaaactttt ggatttcctg aagccatact gcttcttcca
     1681 tttggttggg gatcgtcaag cgtatcacca tctgttcttt ttcaacgcct agactggctt
     1741 ttagttttgt cgctgtcagt gcttcgaata actgttccac actatagcgt tttgttccaa
     1801 ctttacttag agtaaagact atatgcgcct cctgtttcct tatcgtttct aggagcgctc
     1861 gatcgccatc cattttgatc tgcccaattg tcaagagatg tgctacttct tctggatatt
     1921 ctccaaaacg gtcgagcaga tcatcttcta gttcatctag catttcttgc gaatcaagtt
     1981 cacgaattcg tttatagatc tcgattttct gccgttgatc tgccacgtat gtttcaggta
     2041 gatacgcatc gacaccgagg tcgatctcaa cagtggtctt ctccacttga ctattctttc
     2101 cttgtttacg agaaacagct tcgcttagca tttgagaata catatcgaaa ccgactgcat
     2161 caataaatcc atgctgctga gcacctaaga gattacctgc accgcgaatg gaaagatccc
     2221 gcatcgcaat cttgaatcca gatcccagtt ctgtaaaatc tttgattgcc tgaagacgtt
     2281 tttcactgac ttcgttaagg atcttctgct gctcatacat aaagtaagca tatgcgacac
     2341 ggctgcttct tcctacccgc cctcgcaatt ggtataacgt ggataatccc atataatcag
     2401 cgttctcaac gaacaacgta tttgcgttcg ggatatctac tcctgtttca atgatggttg
     2461 tagtgacaag aacatcatat tgaccttcaa taaattcaaa aagcgtattt tctaattgcg
     2521 cctctgtcat ttgcccatga gcataaccga tccgtgcttc cggaacgagt tcttggattt
     2581 cttctacttt ttgttcgatc gtttctaccc gattatataa atagaatact tgtccacctc
     2641 gtcccatttc tcgatggatt gcttcgcgaa tagccccagg atttttttcc ataacatacg
     2701 tttggatcgg ataacgattc gcaggcggtg tttcgattac tgaaagatcc cgcacgccta
     2761 acatcgacat atgcaaagtt ctcggaatcg gtgttgcagt cagagtcaaa acatccactt
     2821 gtgcacgtaa ctgtttcaat cgttccttat gttttacacc aaaacgttgt tcttcgtcaa
     2881 tgatcagtag acccaaatcg ctaaattcaa tatcttttga caaaattcgg tgggttccta
     2941 caacaatatc aacttgacct gtacgcagtt gttcgatggt ttctttttgt tgctttcttg
     3001 ttctgaaacg acttaatagc ccgatattta cggggaaacc ttcaaatctt tccagcatcg
     3061 tttcatagtg ctgctgtgcc aagatcgttg ttggaactaa aaaggctact tgtttgcttt
     3121 cctttatcgc tttaaaggcc gctcgaagcg ctacttctgt tttcccataa ccaacatcac
     3181 ctacgagtag tcgatccatt ggtttttctt tttccatatc ccgtttgatc tccgctgcac
     3241 ttctcaactg gtcatcagtt tcagagtagg ggaaggcatt ttcgaattct ttttggtagg
     3301 cgtcatcagg tccaaaagca tatccttttt ctgactctct tgcggcatat aatttgatta
     3361 gatcgtcagc gatatcctcg atttttgaag agacttttcg cttggttttc gtccattcac
     3421 ttccgcctag cttattgatg cgaggcgctt tggcttcaga ggcgacatat ttttggatca
     3481 gattcagttg agtaacagga ataaacagtt tgtcgtcgtt ttgatacaaa atagtcatat
     3541 agtcttggtg aacgccatct acttctagcg tttccatccc aatgtatttc ccgataccat
     3601 gattggcatg gacaacgtaa tctcctgctt ttaactcatt atagcttttc aatcgctctg
     3661 cgtttgttac tgtttgacgt ctcgtttgtt ttttcgtcgt tttttggaaa atttcttttt
     3721 ctgtgatagc tactattttt tcttgaggaa gttcaaaacc agcctgcaaa gctccttcaa
     3781 ctaattgtac ctgtcctctg atcaatgcat ttttttctgt ttctactact aatatgtctt
     3841 catctcgaag catttgttct gctttgcgta ttctgtcctt tgtcggaata aagatcacaa
     3901 cagtctgttt ttgcttgcgc caacggtcca tttctgtttt cagcaatggc atttgtccga
     3961 agaactgttg catcgtccga tattgaaaat tatggattgc ttggaaacga agatttccca
     4021 tccctttttg gaagagtgag aaaaaggttg tggtgaactt ttccttctgc acgatttttt
     4081 gtatatctgc tgaaaatgtc tgctcagaaa acacccgcaa ttcgctgatt ttttgagtct
     4141 gccattctgc cgcttccctt tcgatttccc gatttgtttc catgattcgc tgataatcat
     4201 caacgactag taaactattt gaagggaaat aatccaagat cgtcacagat tcttggtaaa
     4261 gaagatctga gtagaatttt gcttcttctg tcgggacacc tgcttgccag cttgaagtca
     4321 gctgaccgaa gtaatcttgc aaaaactcgc ggtctgcttc ttctgtggtc gcagcaagcc
     4381 gtttttctag cattttttgc atttgctcta ttccatgttc tagatcctct ggtccgtaga
     4441 cttgttcact cgttggggaa acccagattt cttccgtttt ttcgattgag cgttgtgttt
     4501 ctgcttcaaa ataccgcata gaatctatct ctacatcaaa tagttctaca cgaacaggat
     4561 aggttgtcgt taacgggtaa atatcgataa tgcttcctcg aatactgaat tctcccggtt
     4621 ttgcgaccat ctcttctcgt acatagccca tcaaagtcaa ttggcgagga agattttcca
     4681 cgtctatctc atcaccaata tgccaattca attgcgcctg tagccaagtt ttcttattcg
     4741 gcagtcgttt gtgcaaagat gcgactggca acaaatagat acctgctttt ttttcttgaa
     4801 gtgcgttaag cgttgcaact ctctcgcctc gcgcttctgg agaagaaaag gccatttcag
     4861 cagaaagcac ttcatccact ggaaataaat ggatctgatc ttgcggaatc acatgttgca
     4921 gatcttctgc taactgattc gcataataca aatttggcat cagaataatc atgtttttat
     4981 ttgttttctt aaacgctcga gcaaatagaa gtgttttcgc tgagcccgct aatcctgtga
     5041 ttagttgtct aggcattttt tcttctaaat tgctgaccca ttcttttact tgcggatctt
     5101 ttccgactaa ttgaataata tccattcctt ttcctccttc ctcaattgaa tcgattcatc
     5161 gcatccacga acgaatgccc ttctatgaga tattcgactg ctttaacaga ttgacacatg
     5221 ctttcttcta tcaaaggctg attttctttg ctgaaaggac taagcacatg ctggactact
     5281 gtttttttcc cttctggcct gccgattccc accttgattc gatcaaagac attcgtattc
     5341 aaatgggaaa taatactttt gataccgtta tgcccgccgg cacttccctt ttgacggagc
     5401 cggatttttc ccactgccag atccaaatca tcgtagatta cgactaattc ctctggataa
     5461 atcccaaaat aagtcattag tgggccgact gcacgaccag attcattcat gaatgtttgt
     5521 ggtttgacta aaaggatctt ttctccatta tgaaaaaatt cggcaacttc tgcttcgaac
     5581 ggattttttt tgaaagtagc ttgatgctcg cgtgcaattt tatccacgac cataaagccg
     5641 atattgtgtt ttgtatattg gtatttggta cctgggtttc ctaacccgac gatcattttc
     5701 atttttgttt tccacctaat tctaaaaagc agcacaaaag gctggactgc taattagcgg
     5761 tccagtccat cgagcttttt tcttttttac acttgtcaat gcatagtata acacaaaaca
     5821 aggcgtttcg gctaaaagag aatcgttaat aaatgataaa gaaattaaaa atttcttcaa
     5881 agtttaatga aataattgat atataaaaat agtgaaataa cgcacatgta agcttttaca
     5941 tataatacaa ccttgttatc tgttatattt tatagccaaa tagcgtgaca tggggcttga
     6001 gaaatgttag cataagcatg ggaaaaaaca aatcaatgga aggattgata ctaaatgact
     6061 gcagctgcag aaaaaaaaga tcatcaaaaa gttattcttg ttggagacgg cgccgtaggt
     6121 tctagctatg cctttgctct agttactcaa aatatcgctc aagaagtagg tattgtggat
     6181 atcaatactg ctaaaactga aggagacgca attgacttgt ctcacgcttt ggcatttact
     6241 tcacctaaaa aaatctattc tgctacttac gcagacgcac atgatgctga cttagttgtt
     6301 attacagctg gcgcaccaca aaaaccaggt gaaactcgtt tagacttagt aaacaaaaac
     6361 ctaaaaatca accgcgatgt tgtcacacaa atcgttgaat caggatttaa cggtatcttt
     6421 ttagttgccg ctaacccagt agatattttg acttactcaa cttggaaatt ctcaggtttc
     6481 cctaaagaac gcgtaatcgg ttcaggtacc tctcttgact cagctcgttt ccgtcaagca
     6541 cttgcggaat tagttgacgt tgatgcacgt aacgtccatg cttacatctt aggtgaacac
     6601 ggagattcag aattcccagt ttggtcacat gcaaatgttg ccggcttgca aatctatgaa
     6661 tgggtaaaaa acaacccaga aatcgatgaa gaagctatgg ttaacctatt ctttggcgta
     6721 cgtgacgctg cttacacaat tatcgagaaa aaaggcgcta ctttctatgg tatcgcagta
     6781 gcattagcac gtatcactcg tgccatctta gatgatgaaa atgctgttct tcctttatca
     6841 gtattcatga atggcgaata tggattgaac gacatctaca ttggtgcacc tgctgttatc
     6901 aaccgtcaag gtatccaaaa agtaatcgaa attcctttaa gtgacagcga gcaagaccgt
     6961 atggctgctt ctgctaagca attaaaagaa attctagatg aagcttttgc taaactagac
     7021 gctgaataat ctttgaacat gacggaaaac atctattcgg tgttttccgt ttttttattt
     7081 tatcaccctt atggtttcta aattttttca taaagtagag ataaccttcg tcttttcctc
     7141 taaattatgc tatcatgaga ccgttccacc ctaaactttt acttaaggag ttgtcgatat
     7201 gacaagatct tcgcatcgaa aaaaatcgaa acatgttgct ttgctttcag ttcttggtgt
     7261 tctcttagtg ctgatcagct tttataccta tcgcagtaca tactacacaa agcatttttt
     7321 gccgaataca gaaataaatg gaataaatgt cagcaatcta acagtcgaaa aagcaaatga
     7381 aaaattaaaa gaagcttatt ctgataaaaa actggctatt aaagaagatg gaaaagtctg
     7441 gcaggaagta ccaaaatccg agctcggcta caaagatgat ttcacttcgg agctttctcc
     7501 gatcctaaat gaacaaaacg gatggacttg gggaatgact tatgtctcag cggcagaaaa
     7561 acaagaaata gatcctgtca gtcaaaacca ccaaaaacta gacactaccg ttcagacatt
     7621 gacaactaag ctgactgatt tgaacaaaga ccggacacct actactgatg caacgatcga
     7681 aaaagcaggt gactctttcg ttatcaagcc tgaagtcaat ggaaatacga tcgacgtcga
     7741 tgctgcggtc aaacaattaa aaagtgcggt aaactctggt aaagatacaa ttgaattgac
     7801 tgaattcaaa gaaaaaccaa aagtaacatc tgaagacagc tcactgaaag aacaactcgc
     7861 atccatgaat gctatcgcaa atgtaaaagc tacttacagt ataaacggtg agactttcca
     7921 gatcccatca agtgatatta tgagttggct gacctacaat gatggaaaag tcgatttaga
     7981 tacagaacaa gtacgtcaat atgtaacaga tttaggcaca aagtataaca caagtacgaa
     8041 tgacacaaaa tttaaaagta caaaaagagg agaagttacg gtcccagttg gcacttacag
     8101 ttggacgatc caaacagaca gtgagacaga agcattgaaa aaagcgattc tggcaggcca
     8161 agattttact cgttcaccca ttgtgcaagg tggcacaacc gctgatcatc cattgatcga
     8221 agatacgtat atcgaagttg atctggagaa ccagcatatg tggtattaca aagacggaaa
     8281 agtggccctt gaaacagata tcgtttccgg aaagccaact acacctacac ctgctggagt
     8341 cttctatgtt tggaataaag aagaagatgc aactttaaaa ggaacaaatg acgatggaac
     8401 accttacgag agtccggtaa actactggat gccgattgac tggacaggtg ttggtatcca
     8461 cgattcagac tggcaacctg aatacggtgg agacttatgg aaaacccgcg gttcacacgg
     8521 ctgcatcaac accccaccaa gtgtaatgaa ggaattattc gggatggtcg aaaaaggaac
     8581 gcctgtattg gtcttctaac aaaaataata tataaaaaga aagcacccgt acgattggta
     8641 caggtgcttt ctttttatta tctaacaatg tgctacttac atttcagcag cagtaaagac
     8701 ttctgaaaca tcatcatcgt cttctagctt atctactaga cgttccaatt gctctttttg
     8761 ttcctcattt aaatcaatcg tcgtttgtgg aatcatcgtc aattctgctt gcgccaaagt
     8821 gaactctttt tccaactcat ctcgtacagc agcaaaatct tctggtgcag tataaatctc
     8881 gaatacttca ggagaagttt gcagatcttc tgcccccgcc tctaatacat cctcaaacat
     8941 cgtctcttca tcaacagtca acccatcgcg ttcaatagcg atatatcctt tgcggtcaaa
     9001 caagtaactg actgaacccg tttctcccag tgatccaccg tttcgagtaa aagctactcg
     9061 gacatttgtc gctgtccgat tacggttatc tgtcaatgca tggactaaga ttcctactcc
     9121 acccggacca tatccttcat acgtgatttc atcgtaattc gtttcatcta cggcactcgt
     9181 agccttttta atcgcccgtt ccacattgtc gttaggcata tttgcagctt ttgctttgtc
     9241 cacaacaagc cgcaaagctg gattcataga tggatcagga ccgcctgctt tagctgccat
     9301 ataaatttca cgagaaagtt tctgaaagac tttccctcgt tttgcatctt gggcattttt
     9361 tcttccttgg atattatgcc atttactatg tcctgacatg atttttcctc ccttgttctt
     9421 ttgagaatac acttctattc tagtactatt tcacaaaaaa acaaagccac agccaatcta
     9481 taaacgcttc ttgaatgccg caaaaagcac acataatatc acagcagtca gagcaccgat
     9541 cgaatccaac atgacatctt ggaacatagg tgttcgtcca ccggtcatca tctgatggaa
     9601 ttcgtccaaa cctgcgtacc ctgttgctga aagccaagca aggatcgctg tcagccacca
     9661 aatttttagc ttggggaaaa cacctaaaaa caagctgcct cccaaaataa aataggtacc
     9721 gaaatgtgcg cccttcctta aaaagaactc taaaaatttg gcgtagccca gatggttgat
     9781 actcaccata cttccaccat aattaaaacg tatacccgct aatgtgtctt taaatggctg
     9841 attaggcagc cagctttcta atcgcgaaac ttgtgattgc tgtccgtaag tttgagaaga
     9901 actgtaaaat aagattataa cgactatcag tgcgattact aaataaatat ttccgttttt
     9961 catttgtttt tttgaagaat tcataagtca cctctgtatt ttatcttaga ctttttttga
    10021 agaaacaaca atcattttca tagagacttc ttccataaaa aagtgctata cttaattttg
    10081 tattacaata tgaagggagt tttttcatga gttattcatt aaattggaat ctagattcta
    10141 ttttttccgg aggcagtcat tctgatgctt tgaaccaacg gatgaagcaa ttggaagacc
    10201 aaatgaacga gtattatcac cgcgtaacaa aatggtcacc tagttctgac aatgcagagc
    10261 agttaaacgc tattcttcaa ttgcaagaaa cgattacgaa tggttttacc caatgcagca
    10321 gctatattac tgcattgctt tctgccaatg tcaatgattc agatgcaaag gtcttatcag
    10381 gcaagcttta cgcaatgctg cctcgtctgc aatcagccga aactgtattg tcaaagaaat
    10441 ttgcagaaat atctgacaat gattggaatc agatgctttc tcaatcttcg tttgaaacaa
    10501 ttgcttttcg tttaaacgag attcgtcgtg atggctcaca attattatca gaagctgaag
    10561 aaaacattat aaatacgctt tctttagatg gtctaaatgc ttggagcagt cattatgata
    10621 cgattgtagc cagcatttcg attcccttcg aacaagatgg acaagtagtc gaattatctg
    10681 ctggacaagc cttcaataag atgatgggcg atccagatcc aaaagtcaga gaaacacttt
    10741 tcgcagcatg ggaagaagcc tggaaagaaa aggcacccct cttcacggat actttgaatc
    10801 atttagatgg ttttagactt tctgactatg aacttcatgg tgtcacagat tttctacaaa
    10861 aaccattaga gtacaatcgg ttgaagaaag aaacattgtc tgtcatgtgg gatacaatcc
    10921 agaaaaacaa acaacccctt gttaattact tgactagaaa agctaatctt ttcggtaaag
    10981 aaaaaatgga atggcaagat caagatgcac ctattatttt aggcgatttg aaagaaaaaa
    11041 cctttagttt tgacgaagca gcagcattta ttatcgaaaa ctttaacaaa ttcagtccaa
    11101 aaatggctaa atttgcccaa tctgcttttg aaaagagctg gatcgaagca gaagatcgtc
    11161 ctgggaaacg tcctggtggt tattgtactg agttaccaga aacaaaagaa tcaaggatct
    11221 ttatgaccta cagtaattct gttaatgagg ttgcgacact tgctcatgaa ctaggacatg
    11281 ctttccatag cagcacgatg tgggatttac cttctttaaa tcgcgaatat gccatgaatg
    11341 tcgcagaaac agcaagtaca ttcgcagaac tgatcgtagc cgatgctaca ttaaaagtcg
    11401 caaaaacaaa agaagaaaaa atcaatttgc tagatacaaa actgcaaaat gctttagcta
    11461 tgttcatgaa tatccattcc cgattcattt ttgaaaatcg attctatgaa gcacggcaag
    11521 aaggattagt ctcagaagat aagatcacag agatgatggt tgaggcgcaa aaagaaggct
    11581 atcaaggagc tttagctact taccatccat atttctgggc tgcaaaattg catttcttta
    11641 ttgatgacgt accgttttat aacttcccat acacatttgg ctacctcttt agtttgggca
    11701 tctatgctta cgccaaccaa aagggagcaa attttgaaga agaatatatc catttactac
    11761 gcgatactgc ctcaatgacg acggaagatc ttgcgaagaa acatttgaat gtcgatctta
    11821 cccaaccaga tttttggcaa gcaggtatcg acatggtgac tgaagatatc caaacgttca
    11881 tggaattgac agaagaattt atctgatttc tattttatca caataaaaaa agagttagtc
    11941 cagaatgggc taactctttt tgattaatca ttcttacatg ctgacagcaa agtcaggggc
    12001 aaaccattga actgaaccaa cttttacgct ttcgcctggt tgaggagcat ggatatattg
    12061 tcctccgcct aaagcaatcg ctacgtggta agttccgcct tgtgaccccc agaataacaa
    12121 gtctccagct tttgcttgag aaactgaaat ttttgttcca gctgattctt gaggaactgt
    12181 ccaaccaccg atgtcacgac cagttacttg catgtaaaca tagcgtgtga accctgagca
    12241 gtcaaagcca cttggatctt taccgcccca aacatatgga gtaccaatat atttgtaagc
    12301 ttctgctacg attgctgcac cattcacact tccagaagga gctggagcag gtgttacagg
    12361 ttttggttgt tcaggtgttg gtgttggcgt tggagccgga gtactacttc ctgtgccgtt
    12421 tcctgtatca acactttgat ctgttgatgg tgttgtaggc gcaggtgttg ttgattcttc
    12481 tgttgttgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt caggcactgt
    12541 tgttgattct tctgttgctg aagattcagg cgctgttgtt gattcttctg ttgctgaaga
    12601 ttcaggcgct gttgattctt ctgttgaaga ttcaggtgtt gttgattctt ctgttgctga
    12661 agattgtgta gctgatgatg attcttcagt cattgaagat tgtgtagctg atgattcttc
    12721 tgttgctgag ctttgtgcag tagatgaagc ttgagtagct tgtgcttcag cctctgcttg
    12781 ttcgcgtgct tctttttcag ctttttcttg tgcagcttgt tgacgagctt gttctgctag
    12841 acgttgttgt tcacgaatac gagcttgctc tgcttcagct tctgcttttt gacggttcaa
    12901 gtcagctttc ttgtcttcag cagttgcttg ttcagcagct aatgaagttt tcagtacgtt
    12961 caaatcagct tgtttagata aaagatcgcc tttttgagat tccaaagcag cttggttttc
    13021 agctaattct tttagtttag catcgttttc agcttttttg tcttctacag cttttttatc
    13081 ttgtttttgt tgttccatca agtcgttgtt tgcttttacc atcgttgtca ttgcttgaac
    13141 gcgtccaata gcatctgcta atgaatcagc attcaataca gcatcgatgt agtttgagct
    13201 tgtgttgctt acttgtgctt cgcgtgcttg tttttggatt gtatcttcac gtttttcaat
    13261 acgttcttgt aaatctgcaa tgtcttttac taattgtgca gattcttgac gcaatgtatc
    13321 ttgttttgct aataggtctt gtgcttgtgt gttgatttca gaaacttggc tttctaaggc
    13381 atcgatttgt gattgtgcat ctgcttgttg atttttcaag tctgcaatct tttgatcttg
    13441 ctgttgtatc tgagaatcaa aatcgtccgc cgcggcagcg atcggtgatg ctacggcagt
    13501 caaggtcatt gaacatacca ttactgctga tattaaactc tttttcactc ttcattcctc
    13561 cgactggctt aatttaataa atatctaaat atatttaagt ctttcttaac gatataaaaa
    13621 ttgtaccata gcagtgtgtc aacgatataa tagttatgtt acaaaaatgt ttcaaaacaa
    13681 cttttaaaaa tgacaaaact gttcgtttca gaaaatgatc atgaaaaagc cgcgttaagt
    13741 ctagcatttc ttaagataac aacgattctt tttcgctttc gacctaaaaa taaaaaaaga
    13801 aattattcat cactgaataa tttcttaaag ggaaaaatga ataatacaaa tataatcatg
    13861 ttcaacagca gtgtaggtcc taaaaacctt gtaataaaat acgtgttatt tactactgct
    13921 aatttaaaaa tcaactggac caccatcgta aacaattcat aacccgtgac aaagatgatc
    13981 atactgaaaa attcagtaaa gatgttgaca tggataacgt ctttcattac atacatcaac
    14041 catacaatta aagggagagc tactgcatag ataccgataa cgccaatata gtaagcgtca
    14101 tagatagccc cgagaacaat cgtggtaatc agtaaataac gtttttcaaa cgccatactg
    14161 caacataata atgccaatat cagaaagtgg gcatttgaca tataggtgcc tttcgaccac
    14221 tcccctagca ttcttgttaa atgaccatct atcaacatca aaaagaacaa aacgattggc
    14281 agatagtact tcattgtttc tttcttcaac atggactaat cctccgctag ccgttggaca
    14341 atcgtcacaa ctgacacgtc atacatttct gcataaggtt tcacataaac ttcccgatcc
    14401 agcccataac tatctggttt cgttttaata accgtaccga tcggcaagtt agctggagaa
    14461 ttccctccaa gaccggatgt ctggacaaca tccccttctt tgatatccgt atcaccagtt
    14521 aattgggtca ccactaaggc atggagcttt tcatcatagt ttttaagcaa accaaacgct
    14581 tcgccattag ccgaagatac ccgtactgga aaatgattgg agctttcatt agaggatgac
    14641 agtaattcga ttttagacga agccgtattg acctcgatca ctcggccaat caatcctttt
    14701 tgcgacatga ctgccatatt tgcttcgata ccgtctttag aacccttgtc tacgatcagc
    14761 atgtcctgcc aactgtcagg cgaacgagta atgacatttg ccgttacttt ttcgtaacta
    14821 gtcagcgtct gattcaactc aagttctttt ttcaacgctt cgatttctct ctcgtaattc
    14881 ttgttttgct gtgaaacgtc atcatagcta tcgatttttt cttttagccg ctcattttcg
    14941 gcataagttg aaaaaagatt atggacagaa tctactccat ttcgtaccca ccgtactggg
    15001 gcagagattg ttttatcaac cattgcaaca ctgtcattaa cgatggattg gaagaaattg
    15061 ggctttcctt cattcgctcg ctgcgcagcg gtcacactta gtatcgtcac cacgatgata
    15121 acaataacca aggtaataat aatattttta ttaggattga acttcttcac aaagacacct
    15181 cgaattaatt agactcttgt gttcaatttt atcactaaat gccgtgaaac aaaaggtaag
    15241 ttgctcctct taagcttttt tttagcaaat aatagattct gtcattttta gcctgaaaga
    15301 caaatttttt tatttgttta tggatcaatc tttgctttga ataactgcaa tcataccatc
    15361 tgaaaaagaa aattctgcgg tatctgaaag aaattcgtta cttgcataag atgtcggata
    15421 ttctacttct ttattttcca atacatactt gctgcctttt aatgtaagat tcgagacggg
    15481 agtcaagcag caataagcta aatacttcat atctggtatc tgtcttacta tatgctctcc
    15541 aggcaaataa taagacaaat aattttgaca atctctgata ataagctgat gggcaaaggg
    15601 tttgaagcga ggttccatac ctaaccagag attggctaaa agatgatcca atcttccgcc
    15661 cgtagctcca atcaaagtga cttccgcttg cgggtaacgt tcaaatgttt ttaagatacc
    15721 taactgtgta tctgtatcgt ctttttctgc aggggaacgg gtatactctt tggcttttgc
    15781 aaatacttcc ttctgctctt cgttcgacaa tgaatcaaaa tcacccactg ccagatctac
    15841 tgggtagcct gcttgaagca ggtacagcgc accacggtct atcccgacat aggcatcgta
    15901 tttggacagc agagactgcg gccagttttg cggattacct ccagcagcta ataaaatttt
    15961 catttagtct aatgcctctt ttaatcgttc gatttgtccg actggatttt cggctccata
    16021 gatatatgat cctgcaacga agacatccgc ccctgcatct ttacagattt ttgctgtttc
    16081 tggaacgatt ccgccatcta cttcgattgc ataagtccaa ttgttctctc tacgtagatc
    16141 agcgagttcc gtaatttttt ctactgtttc ttcaataaag ctttgtccgc cgaacccagg
    16201 attgacagtc atgactagta cttgatcagc caaaggcaaa acatgtttga tcatggagac
    16261 tggtgttcct ggattgataa ctactccagc tttaactcct aagctttttg tcatttgaag
    16321 tgcccgatga atatgaggag ttgcttcgac gtggacagta atgatatctg ctccagcttt
    16381 tgcaaatgct ggaatgtagt tttctggatc aacgatcatc agatggacgt ctaacggtaa
    16441 tttagtaaca ggacggattg cctgaacaat atttggccct aatgtgatat ttgggacaaa
    16501 tcggccatcc attacatcta cgtggatgta atctgcgcct cctttttcca ctaactcaat
    16561 gtctctttgt aaattggcaa aatctgcact caaaatggat ggtgctatct tcattcttgt
    16621 ttcctcctat tttcgtttac cttctttttt tgcatacatt ggttttcttt tttcgatttc
    16681 ttgtaagaat tgcaaataat tgtcgtaacg tgtctgtgcg ataccacctg tttctactct
    16741 atgttttact tcgcagccag gttctttagc atgcatgcac tctctaaatt tgcaatggac
    16801 agaagcttct acaaattcag gaaattgtct cggcagttct cctgcttcaa ttgtcaaaaa
    16861 gtcaatcgag ctaaatcctg gtgtatctgc tactaacccg ccatagatag gaatcagttc
    16921 cacatgacgt gttgtatgcc gtcctcttcc aagtgcatca gaaatctctc ctgttgctaa
    16981 attcagttcc ggtgatattt gattcagtag cgtcgatttt cctgctccag actgacccat
    17041 aaagaccgat agccgttctg ggaagtaacg gatcagttct gtcgtatcac ccgtatgatt
    17101 gggtacaatc accggatagc ctattttagt ataggtttgc ttgatctctt cgattttttc
    17161 tgggtcgtct gacaagtcgg tctttgtcaa aaaaataatc ggttcgatcg actcgtattc
    17221 caaggtaact agaaagcggt caagtagatt ataagaaaat gcaggctcta cgatacttgt
    17281 gacaatgatt ccaagatcaa catttgtgac tggtgggcga actaattgat tttttctcgg
    17341 caagatttcc agaagatagc cgtctgtttg attttcactc tcaaagatca cctgatctcc
    17401 gacaagcggc gtgattttcc gattccggaa atttcctctt cctcttgttt gataggtctc
    17461 tccgtttgcg tatacatagt aaaaaccgct gattgctttt ctgatttgtc ctttgataag
    17521 tgccacctcc gtttttgttt attttaccat acgaagagga aagtaggtgt tattccatgc
    17581 aaacttctca aagtatatgg cttgaaaatt gatcaaaaga gatagaaaca caaaaaagag
    17641 aatgcaacaa atcaatgtcg cattcccttc tgatatattt tattcagcac ttattttgaa
    17701 ctttctgagg tgctagctga tgaacttgtg gagctggaag attcttcttt tgaagagttg
    17761 cttgtgctac tggagctact tgagctgcta gaactgtctg aagagtcttt ttcagaacta
    17821 gaagacgctg gatctggtcc tttagagtaa attacgttca caaccattcc tttggtaatt
    17881 gtcgttcctg cccctggtac tgtacgaatc actttatctt tatccaccgt gtttgaatat
    17941 tcggaagtct catgcccgat atattctgca ccgacactag ctaaataact ttgtgcttcg
    18001 ctttttgtat agccggagat atcattcaat ttaggctctg ttccttggct tacgacaaaa
    18061 ttgatgtaag tttcttctgc tacaacttca cccgaggctg gatcctgact gatgatcgaa
    18121 cctgatggta cagtgtcgct atagtcataa gtagcactaa tattattgat attgaatcct
    18181 tgtgtaacaa gccaattacg aacagtttcg tagttttctc ctacatgact actcaaagtc
    18241 actgtttctg gtcctttgct gactgtgaaa gtaatcgtgt ctgttttagg atcgacttct
    18301 gtatcttctg atggagattg cgaaatgatc attccgcttt ctacctctga actgaattcc
    18361 tcttcttttt gaatcaagtc agcagaaaac cctaattctt tcaaacttgc ttcggcatct
    18421 tcataacttt taccactgta atcatctaat ttgatttttt tcgtgccgct actaatatac
    18481 agagtgactt cgcgtttttg cttgactgtc gttcccgctg ccgggtctgt cttcactaca
    18541 ttaccttctt cgattttgtc atctgcaatt tcttctgttt ttgttgctac ttctaatttc
    18601 gcttttgcga gtgtttctct agcagaagct tcagaaagat cagaaacatc aggaacaaca
    18661 acttctcctc gcccgccaga agcaaagaat acagctgttc cgattcctaa aacagcaaga
    18721 atagctaata ggatcagcca ccattttttc tgtcctttct ttttaggatc aggcagaact
    18781 tcttcctcct ctggtaaggt ttcgttttct ggcttgtctt ttggcagagg catagatttg
    18841 aatgaatcag gcattggtgc atcttccgta aggggtgtga taacttttgt ttcattatcc
    18901 atcacatgag gctgccattt ttcttcgttt gcacgagctg gtgataatac tgtagaaaga
    18961 tcatcagaca tttcctcggc tgacttataa cggtctgcag gttctttagc cgttgcccgt
    19021 aaaatcacgt tttccagtgc ttgtggcaca tttggatcaa gtgcttttat agatggaaga
    19081 tcatcttgaa aatgttttaa agcaattgtt acagcagatt caccatcaaa cggtacgcta
    19141 cccgtcaaca tctcataaag gatgatccct aaagcataaa tatctgactg cttcgttgcc
    19201 atacttcctc tggcctgctc aggggacaga taatgaacag atcctagcat cgtattcgtc
    19261 tgcgtgatcg atgtttcaga taaagcgata gcaatcccga aatccgtgat ttttacaaca
    19321 ccttcattat cgatcagtac gttttggggt ttcaaatctc ggtggatgat ttgatggtta
    19381 tgagctaaag aaatagcaga aaggatctgc tgcataatat ttactgctgt ttcataagga
    19441 acaggataat gtgtttggat atatcgtttc aagtccattc ctttgacgta ttccatcacc
    19501 agatactgca tgttgtcttc ttcaccaaca tcgtaaacac taacaatgtt tggatgaacg
    19561 agttctgttg ctgctaaagc ttctctctga aatcgtcgaa tcgcagtctg gtcattttga
    19621 aagtcaaatc gtaagacttt aactgctaca tcacgatcca agattaaatc gtgcgccaaa
    19681 aatacatttg ccataccacc gctaccgata tttccagtga tttgataacg gccatttaat
    19741 tttttgccca actcgatcat acactgtcct ccttatattc gataagaaga acggtgatat
    19801 tatccgctcc gcctgcttca ttggcacgat caatcagctc agttacttta tgagacaatg
    19861 ggccttcttg attgatgatc gttcctattt cttcttcaga cagcatgttc gtcaagccat
    19921 ctgaacataa cagcagtcga tcttttagtt gccaaagatg agtagaaaca tctacttcga
    19981 ctgttccagg catgccgaca gaacgtgtta aaatattttt ccttggatgg ttcactgcca
    20041 tttcttcgct gatttctcct gatttgacta gttcattgac taaagaatga tcctccgtca
    20101 attgcatgat tttatcttca cgaatcaaat aagctctgct atccccaaca ttggcaatcg
    20161 tgaaccagtc atctgacaga gcggccgcta cgattgttgt tcccataccg ttatattccg
    20221 gctgttcttg tccacgctga taaatcagca tattttcttc ttgaatcgat tgaatgaacc
    20281 attgggcgat tttttcatcg tcaacagctt cttgtttttc ccaagcttcg cctagattcg
    20341 ttaccgccat ctgactggca atgtctccgg cacgatgacc acccatcccg tctgctaata
    20401 cagcaagctt gagacctgaa cggctggtaa atacactggc atagtcttga tttgtgttac
    20461 gtcgtcttcc tacatctgat tgatattcaa tctgcatttt ttcacctcat tactttttgc
    20521 gcaaacaaca aatgaaaaat ccatctgtgt aaaactgatg cggatacaaa gtcaacattt
    20581 tctcttcgat tgatgattgt agcataggat tggtcaaaac atcaatcctc tcaaaatcag
    20641 ggtgtctttc caagaaagca gaaacaacgt cttggttttc ttcttttaag atcgtacaag
    20701 tgctataagt cagaataccg gaagacttta aagttggtgc gcagctttct aaaatttcca
    20761 attggatttt tggtaaattc gctaattcat ccggctgttt tttataacga atatctggtt
    20821 ttcgccgaag cagcccaaga cctgaacaag gcgcgtctac taaaatccga tcaaactgct
    20881 ctgcttcgaa ctgtttactg acttctctag catccatctt ttgagcgtag acagcagaag
    20941 agacacctag acgtgctgca ttatcttcaa tgagtttgac tttgtgttcg tgtacatcta
    21001 aagcgaccac ttctccaccg catgacggat ctaaaaaaga agcaatatgc gtcgtcttac
    21061 ctccgggtgc tgcacaggca tctaagacat gatgttcagg ctcgatttgc atagacggcg
    21121 ctacaagcat cgaactttca tcttggatcg tcatgacgcc atcgtggaac aatgagctag
    21181 aagctaaatg cccttttttc gctactatac cgtatggact gacttggctt ttttcagctg
    21241 tgatctcttc ttcttgcaaa acagtgatgg cctgatctct ggaaagtcgg cgtgtatcta
    21301 ctcgggcact agcatggcta ggctgatata gcgataaacc gagcttttca gtttcttcgt
    21361 atccgatttg atccaccagt cgtttcgtca tccatagagg caggctgatt ttgacagaaa
    21421 gtctttcgat aggatcagca atgccttgca gatctggtgc tccttgacgc tgataattcc
    21481 tcaagacacc atttacaaat ttccctgtcc caggatttcc ttttactttt gcgatttcga
    21541 ccgcctcgtt taaaatagca tgtgcaggga ctttatctaa aaattctaat tggtatacag
    21601 aaagataaag aagcatcttg acccagtcat ccactttttt tgctttagcg atcaatggtg
    21661 caacgtaaaa ttccaatagt cgctgatggc taatcgtacc atagatcaat tctgtaaata
    21721 aacccacatc tttttgattt agctcgcttt gtttgatcat ctcattcaac aaaagattcg
    21781 agtaggcgcc acccttgttc actcgctcta atgtcagtaa tgccatatat cgtactgatt
    21841 ttttcatttt tttagggatt tttttcatta tttcacctgc tgaccaatct gaacttgctg
    21901 accgcttccg ttaaggaaat catgtacggc ctgttttcct ttaccagctg gctgtaattc
    21961 attgatggct aagacggtct gtttaccgca agcaatccat agattttttt tgtctttccg
    22021 gatgatcgta ccaggtgctt tgtctgtagt ttctgccaat acttcaaccg ataataattt
    22081 ccagcgtatg ccctcatagg tcgtgaatgc aattggccaa ggacgcattc cgcgtacttg
    22141 gttgtcaatt tcttctgcag ttttatgcca atcgattgct tcttgttccc gtgtgatgtt
    22201 tggcgaaaaa gttgcttctg actcgtcttg aggtactggt ttttgtccat ccaaaatatt
    22261 gggcaatgtt tctagcaata gctttcttcc taaaaggcta agcttttcaa acatcgtccc
    22321 tacatcatct tgtttagtga ttggtatgct ttcttgcgca tagattccgc ctgcatccat
    22381 ttttttgatc atttccatga tcgttacacc agtttctttc tccccattca tgatggcata
    22441 atgaacaggc gcacctcccc gatatttagg aagtaaagaa gcatggacat tgattgcccc
    22501 taattttggt acttgcaata acttttcagg caagaattgg ccaaatgccg cagtgacgat
    22561 cacatcaggc accaattcaa tgatttcttc catttctggc gaaccggaga ttttctctgg
    22621 ctgcagcaca cggataccgt gcttcagagc cgcttttttt accggtgtcg gcgtaattac
    22681 tcgtttccgc ccaactggac ggtcgggctg tgttaccact gcctggatct cgtaacctga
    22741 ttctatcagc ccttcaagaa tcggcacaga aaaagcaggt gtgcccataa atactagctt
    22801 agtcatcaag atgttcctcc atatattctt caagttcttc ttccgggatc tgctgtatca
    22861 ttttctcaat gaacaagata ccgtctaagt gatcgatttc atgctggaag gcacgggcta
    22921 aatagccaaa agccgtcact tctatctctt ctccgtctcg atcataataa cgcacggtca
    22981 cttcatctgc tcgcttgacc gttccataga catgcggaat actgagacaa ccttctacgt
    23041 caaggctttc accttttgct tcgatgattt ctgggttaat cagttcaaat ttctcgcctt
    23101 catctacttc gatcacagca attctcttgt tttgtcctac ttgaggagca gcgatcccta
    23161 ttccgtcatt cgcaatcatt gtctcatata aattgtctaa taaagcgatt gtctcgtccg
    23221 tgattacatc gattggctga gcagttcttt ttaatttatc attagggtgg atcaatatag
    23281 gatagcgcat aatttggccc tttcttaaat aaagtttaat ggttcgttgt caattgccac
    23341 aaaaagacct tgtcgctgtt ctttctggct tccattcaaa atttcatcca aaacatgagg
    23401 aagattcggt tcatgtttat atttgatgat tagttgataa taatatcggt ttttcactct
    23461 caaaatcatg ctgggcgttg gccctaatag caagctttga gggcttaaca cttcttttag
    23521 ctgattggca atctgaaaga ttttctttgc cgctacttgt tcttcaggat ggctggcggt
    23581 tattttcaca gtaaagtaat aaggaggata gcctccacga tgccgtacat gcatttcctg
    23641 ctgataaaac tgctcataat tttgtttttt agccaattct atggcgtaat gttgaggatt
    23701 gaatgtttgg atcacgactt cgcctgcttt ttccgctcgc cctgctcgtc ctgaaacttg
    23761 ggtaagcaat tggaatgtat gctcactaga acgaaaatcc ggtaaattca gggaggtatc
    23821 agcattcaac accccaacta atgtcacttc tggaaagtcc aaacctttag caatcatttg
    23881 tgtccctaat aatatatctg cttcttttgc accgaatttt tgtaaaatct gttcgtgtgc
    23941 cccttttctt cgagtggtat ctacatccat acgcaaaatc ctagcttgcg gataaagttc
    24001 tcggagttcc tcttctactt tttgagtacc tgttccatag tagcggattt tattgccgcc
    24061 acaattagga cagcgattgg gaataggttc ttcatgtccg caataatggc agcgcattga
    24121 acgtgtatcc atatgcagtg taagagagat atcgcaatta ggacatggaa gaacaaatcc
    24181 acagtcccta cacatcacaa aagacgaata tcctcgacga ttgagcagta ggacagtttg
    24241 ttcttttttg tctaatcgat tttgtatttt ttcctgcaaa ttagcagaaa aagtagaggt
    24301 ccgatgattc tgaaactctt cacgcatatc tacgatctca atagctggaa gctgtgcagc
    24361 tgctttggct cggtggttta actgcaataa ctgataaacg cctttttgag ctcgtgctct
    24421 tgattctaaa gagggcgtcg cgcttcctag aactattggg caatgatgat atttactgcg
    24481 ccaaattgct agatctctcg catgataacg cggtgtctca tcttgtttgt aacttgattc
    24541 atgttcttca tcgataatga tcagcccgat atttttcagc ggggcaaaaa tagcagatcg
    24601 tgcgcctact actacttgcg cttcttcccg ctcaatcttg cgccattcat catatttttc
    24661 accttgggaa agtccgctat gtaagaccgc aactgattct ccgaagcgtc ctttgaatcg
    24721 ctcgaccatt tgcggagtca atgcgatttc aggcactaac ataatagccg ttttattttc
    24781 tgacaaaaca tcagcgattg cctgaagata tacttctgtt ttcccgctgc cagtgattcc
    24841 ctccagcaaa aagacctgat ctttttgctg ctgacctgct gtgatgattt gttcgacagc
    24901 atttttttgt tctgcattca gttccaatgc cgtcgtttgg tcaaatactc ggtctttata
    24961 agggtcgcga taacgttctg attctataaa ctccagccag cccttttctt ttccttgatt
    25021 caagacagca gtactgattc ctttattttt gaaataagca attggttctt cttggctcag
    25081 ttcagataaa cacaaaagaa gttctttttg tttcttggca ttttgccgta attttgccca
    25141 ttcttcttcg atttgctttt cagttaacaa agaacggacc aaacgaatcg tttttacttt
    25201 atttcgtgta tggacttcat atttgatatc tactaattgc tcttggcgcc agcgaatcaa
    25261 ttgagaaagg ctgccttctt cttctgcttc cttccaggaa atcacttctc gctgatcaaa
    25321 caatacctga acttctggtg tgtctgccaa aggatagatc aatttatcat attctgcttt
    25381 catcacactc ggaagcatcg tttgtagaca agtgattttg aatgcgtaag taattttttt
    25441 catataatca gctaatgaaa gtaattcttc attgaccacc ggagcaaggt ctagcaaacg
    25501 aataactgcc ttcaaagatg gattagctga ttcttctgat ttttggatag ccataacaaa
    25561 tccttggaca tgacgattgc cattaccaaa agggacttct actcgcatcc cgacttgcaa
    25621 agctgtttcc atttcagagg gaaccaaata agtgaatggc tgatcggtct gcatagtagg
    25681 tacgtccaca atgacttcag ctgttaatgt catggttcgt ttcctccttt ttttgtcttt
    25741 tcttatcaag cgacaaaaag ttccttgcct attttactag agatcgatgg atttgtctta
    25801 tcttctgcca aatcctaaca aaaaattaac agcagataag aaaaaaaaga actgtgtcaa
    25861 aaagttcaga cacagccttt tctaccagac aataagtggc gaatgtttga aaaggaaaca
    25921 gctgggtttg cttttctttt tagatgttcg ctctattttt ctttattaca ttttttgctc
    25981 ttcacggata cgtgattcca attcgtgttg ttcacgctct ttttgtgctt gacgttcctc
    26041 ttcttccatc ttcaaacgtt cacgttttaa ttcaggatgt ggatcattga ctacatttcc
    26101 tgcatcgatt tcttctaaag cttgaccgac gctttttact gaatcaaaag aatctagagt
    26161 aggctgtgca cttgcatcca attcatgcgc gcgtttactt gctaaaatca ctagagaata
    26221 ttttgaattt acacgatcta atagtgagtc aattgaaggt tttaacatca taagactaca
    26281 tctcctttaa cattttaata tatttaccga ttacgcgatc tactcgaaaa tgttcactgg
    26341 caataatgtt tttgattcgt tcaactgcta gcggcacttc atcatttaca acagcatagt
    26401 cgtaaagagc catcatttcg atttcttctt ttgcaacttt cattcgtttt tcgattactt
    26461 catctgcatc tgttcctcgg cctacgatcc gtgattttaa ttccgctaaa tctggcggag
    26521 tcaaaaagat gaatacacca tcgggcactt tttctttgac ttgttttgct ccttggactt
    26581 caatctccaa gaaaacatct tttccttcat ccaatgtctt gttgacatat gacaaaggag
    26641 ttccataata attcccgaca tattctgcgt attccagcat ctctccagct tcgatcattg
    26701 attcaaactc ttctttgctt cggaagaaat aatcaacgcc ttcgacctcc ccttcacgca
    26761 ttttacgggt agtcattgag atcgaatact gaaaatcatt atcctcactc tcaaaaattg
    26821 cttttcgtac tgtgcctttt cctacaccgg atggaccaga caacacgatt aataatccac
    26881 gctctgacat gacgacgtcc tttcaactag tctttttgta cttatcgttt attttgcact
    26941 ttttttctgc aactttcaag gttatcttta attatagcta gaaattggta aaaaagctac
    27001 cctgtaccaa gaggtcgtaa aaaagctgtc ctgcatcaag taataagaac agccaaacat
    27061 aaacagcttc tcatgttttt cgtttggttg gggttattat cgcagatgca agcttttgaa
    27121 cgccgtgtat aagaggtcgt gaagaagccg attagtttcg agtatgaaga aaaattttgt
    27181 gaaaatagtt tctcacattt ttgcataaat ttggcttaat accgagaaaa tggtttttga
    27241 acatcactta ttcaaaataa aaaaagattg tgacacgaat tttgtcacaa cctttaaatg
    27301 tattcctaga gcaaagaagc taagcgaaga aatccaattc tgtttattta ttgtatttct
    27361 ttgttcagca tccttgctaa aagcccatat tggacaaagt attcactaca cccatgatct
    27421 catcttttcg agaacgtgct ttttctgtcg gatcttctac ctcttctgtg tcaattcgaa
    27481 caataaattt ccgattcatc gtcacgtaag caccgtcgtt gatcaaaata aacagttcgt
    27541 tctcattata gtctgcacag gcatctttcc atacgtcaaa gccgtttttt tcagaaggga
    27601 tatcccgttt aaccacagtt tttccactta attcgtggaa cgttacgtca taatatttca
    27661 ttatctctac ctctttcgtc attcaatatt ttgtacttgt tctctgattt tttctaagat
    27721 cgtcttagac tggacgactt gatttttaat ttcgattgca tttgatttgg aaccgatcgt
    27781 gttgatttcc cgattcattt cttggatcag gaaatctaat tcccgaccta caggacattg
    27841 tgcttcgata agttcatcta gtttttgtac atggatcgcc agacgatcca gttcctcgtg
    27901 aatatctccg cgttccaaca agatggctag ctccgtcaac aaccgttgct catcgacttt
    27961 cgctccaaga aattcctcca gctttttttg ataccttatt tgaaattcct tttcatagat
    28021 ttctacaaaa ctatcgagtt tttttagtac ttctttgaat tcttcagtat tctttttcaa
    28081 cactaaagct aaagcttgac cttctttttc tcgactcgcc tcaatagaat caagtgcgct
    28141 tttcaatgta tctagagcta actgtgccaa ttcttccatg ctctcttccg gtttttcttc
    28201 taccgttacg aagttttcgt ccagcacgaa gcgttccgcc atccgactaa ttgacacttg
    28261 attttgacta ccgtatcgtt gttgaattcc gtctgtcaat tgagtgataa gtgaatcaat
    28321 caattcccag ttgatgacga tctgtttttc gtttttgcca atataagcta gattcacaaa
    28381 tacttcgatt cgaccgcggc ttaactgctg tttaataagc tgacgaaaat cgttttcaac
    28441 aaagttcagg attttaggac ttcgtatttg aacatctaga aaacgctggt tgacactttt
    28501 tatttcaata tctaactgat acgcttttgt ttcgcggatg gctttgccaa aaccagtcat
    28561 acttttcatt cgacagggtc cttcctcatt acatattttc tattgctttt tgcagttgtt
    28621 caaactcttc gttcgtcaat gtcaggcctt tccccatttt ttcatggtct ggcgcccaat
    28681 ctcgaagatc gaattttggc gggcgaccat tccagctgac taggttcagt tctttacgcc
    28741 accccttgtt gttttctgac aatatagcga tttcttctac gatttcatat gaaaattctt
    28801 gtgccatggt tgttcctcct tgagataata taaatatagg atcagtaatg tgtcaactgg
    28861 ttgcttaagc aattcatgct gcaaacgatc cttgcgtttt ccttgtagca gataatcaaa
    28921 ataaacagtt ttcgtgcgct cattttgtat tttttgctgg tattttcgat accttcgcca
    28981 ttcttcagta cttaattgac caatttgtgc caataaatca tccggcatac tttttatatc
    29041 ttgttggcag atcatttgaa tcatcatgtc tacttgatag atttcattgg gcagtaaaaa
    29101 atgttttttt gctaaaagtt ctagcatcgc caatccgcat gtctctggta cgaccctgtt
    29161 cttatataaa ttacgcagtt tttgccaaaa ttttatgctt ttgaaaaaag ataattctaa
    29221 ctgaatttct tttgttaagt aagacaaaaa tcctcgccag tatgttcttg caggtttaac
    29281 agaatgaaat gtataccagt tcccatagta atagcctaat cgttttttca tttccagata
    29341 gcctaattgt aaatttaatt ggttcctttg gctatcaaaa agcagaaagc tgcctagtgt
    29401 ccacaatgtt tgacatttcc aatggataaa aatgtctggc atacgaattt ttttcgctgg
    29461 gcctggccct tgcacatcta cgacaaatgc ctctgttgct cctttgttaa tcgcaatatc
    29521 aataggtatt ttgttgcgat acccaccgtc tgcatattta ttttttccga tctttcgata
    29581 agccatagcg ggataaaatg aagcagaggc gagtatccaa tctggtattt cttctgtttt
    29641 tgtctgttgg atatcagtca ctacttccgc tagttctggc agtttagtag acaccgtata
    29701 taaacgtatt ttgggattat gtttgagtaa ggacaaatcc aaattgtctt tgaccatctt
    29761 ttccaatgga gcagaagaaa ccccgccttc aattagtgct gttttcgtca tttggcgtct
    29821 ttctcgatag aagcgttcac gaaggtcttc aactgcagcc atttctggta acgcaagcac
    29881 ttgtcttgtc gacagctttt tccataaatt cacagctttt ttcatatctc cttgtaaaat
    29941 caatgcgccg ttcaacgcac ctaccgaggt acctgtgata atctcaaagt tgatatcatg
    30001 ttccttcagc gcctgccaca caccaatctg ataagcaccg tgcgctcctc ctccacctaa
    30061 aacaagtgct gtatgataga ccagcttttt atttaattgt gtggttcctt gatagccgtt
    30121 tttatgaaaa atacgcaacc attcttctga tagaggagat tgaaataaaa aacaacagtt
    30181 ttctttaata aaaaaacgag cgcaagcttc catataccga aaaaattttt tccattcaaa
    30241 agtttcacct tcaatcaaca gattattaaa aataacatct cctctacttt tagcaatagt
    30301 aaaatagacg gtatattttt tcgatactgc tttaaaagca cattgctttt ttgcagaacg
    30361 ccaaaaagcg tctctacttt cttgatgact ttgaaaaaat ggaaggggtt gttttgcttg
    30421 ctggataggc gaatacagcg acattttctg tgaaaagtaa acttttttta tctccatcgc
    30481 gctctccttt ttcttcaagt ataccagtac gtcataaaaa ggtcatgagt ctcgtattcg
    30541 actttgtaga ttccataaga aaacaattca taaaacctcc tttaaataaa aaaatacaaa
    30601 aatcacaaaa attaattgaa aatgagagtg ttttcaatga aaacgtgatt ttgtaatgga
    30661 attttaaaat aaaagaaaca agctttttta atgcaaatgt tgtaaaataa aagagattaa
    30721 gaggaaataa gaggaggaac atgatgactg agtcagttta tgtacatatt gataccacaa
    30781 gtaatgctgt tttaacaaaa gggctgactg ctactgactt tgcaaacagt atcgtgcatt
    30841 ttcctcaaaa cttgctcttg cttgatccat ctgcttctgc tggagaatat gaatcccata
    30901 cagggctgaa agtaatccgt ggtacagaaa atattcagcg tttcttttcc tcttctcgta
    30961 acaggcgtgg attcgatgaa ttgaagtgga tcgactttac tgatttaacg atgctgaaag
    31021 aattgacacc tttagaaatt tcagaattat tatattttgg tcatatgaaa acacatcttc
    31081 attcgccgtt tttttataaa ctacagaata attttgtata cttcgatctt aatgatcagc
    31141 tgaatcggat ttattatcgc tatttagatg aattttatag gatttttgct agtaaattaa
    31201 gttcgatctt gtcagataag atcaatgtaa aaaaatcttt tttcaaaaaa gaaacacctg
    31261 ttgaaaaaat cagtgtggaa ttattgaaag aaatgcgtaa tttgatgcag gaagggatca
    31321 ttttttcttt tgatcaagtt ggtttgatcg atggtaaata tcgtatccca atctatgtga
    31381 ttgaagatta tatttggaaa ttaaaaggca cccggtatcg agaagaggaa atcatcgcta
    31441 ctttaatcta ctctgtcacc gatcgacact ggcaattggt agaagatgcc gatctttctt
    31501 atctgccatt gaagtagatc tatttcttat caaaaaataa aaaggaaaca agaaactagg
    31561 acatgattct gtctcaagtt tcttgcttct tttttattta cagctcctgt cgtttatctg
    31621 gcacaaaaat cgaaggagtc gtttgttgga tccctaataa aacgatgatc gttacaatag
    31681 ctgtactcaa cccgctggca ccattcatta cgaaagaaaa gagccaagga ttcatccccc
    31741 atagtgcata cgctccccag aaaaccactc ctgcaataaa atgccaaaaa tatctggaaa
    31801 gtgcgccaat caatgcacca aacacgatgc tttttctaat ttgtttcgta ttatcgtcta
    31861 catagccttt ttggacattt gaagccatga gccctgctgc gcctgcaaaa gtataggcaa
    31921 tcgggtactc aatcaacact tgaacgacac ttaaataata aacttgtccc aacggaaaat
    31981 gtaggatgcc ccaaatgaaa cctgccagca tccctgcctt tgtccctctt cttaacgcaa
    32041 aaagagtcaa tggaatctgc cctaatgaaa tagaaaaact gctgccgatc tggatcggaa
    32101 tcaacgacaa caccatcgct aacgctgcaa caatcgttcc ttctacccaa actctatttt
    32161 ttcccataaa aaaacttcct cctttgaata aaaaagtcac aaaggaggaa gcagctttcg
    32221 aaaatagtca aacagctgtt tattcacaat tcctacgctc gcataaccga tacaggtacg
    32281 aagggtttag aaatagattc aatctcagcc caattggact cccctttgtg agtttattta
    32341 ttatttattt tttgtttcaa acaatggact aactggtttg ttttcatgga tacgtttgat
    32401 tgcatctccc attagtccgc caacacttac ttcatcgatt ttgtcgattt tacgatcttc
    32461 tggaagatag atcgagtctg taacaactag acgctcgata gctgaatctt ctattctttg
    32521 taaagcaggg ccggataata ctggatgtgt acatgaagca tacacactag ttgcaccagc
    32581 ttctttcaaa gcattggcag ccaatgtgat cgtacctgct gtatcgatca tatcatcgat
    32641 cagtacacaa gttttgcctt tgacttgtcc aatgatattc attacttctg caacatttgc
    32701 ttttggacgg cgtttgtcta tgatcgcaat cggagatttc aaaaattccg ctaatttacg
    32761 tgcccgtgtg acaccaccat ggtctggtga aacaacgaca acatcatcgc ctttgattcc
    32821 tttttctaag aaataattgg caataagtgg cgcacccatc aaatgatcga ctggaatatc
    32881 aaagaaacct tggatttgga ctgcatgaag gtccaaagtc agcattcttg ttgcaccagc
    32941 tttttgaatc atgtttgcta ctaatttcgc tgtgattggt tcccgtgcac gtgctttacg
    33001 gtcttgacgc gcatagccat aataaggcat gactacgttg attgtttttg cacttgctcg
    33061 tttcagtgcg tcaatcatga tcagcaactc catcaagtta tcattgactg ggctgctcgt
    33121 tgattgaata acatagacat gcgatccacg aatactttct tcaatgttta cttgaatttc
    33181 tccatcacta aattgagtaa cagatgattt tcctaactcg acgccgactg ctgctgcaat
    33241 tttctctgct aatgggcgat tggaatttaa cgcaaaaatt tttaatcttg ggtcaaagta
    33301 atgttttgac atggggacct ccactttctt tttcacaact ctagaattct acctaataaa
    33361 tagtagtcat attttttaaa taaatcaagt gattttgcaa aagtcttagt ttagataagg
    33421 gagttttttt gcatatcctt ctttattgac ttgtcttgct ctcgcaattg ccagcgcata
    33481 ttctggaata ttgtctgtaa ttgttgaccc ggcagcaatc gaactgtttt tagctacctc
    33541 gacaggtccg atgatattcg tgctagaacc aataaagctg tggtcgccga cagtcgtatg
    33601 atgtttgttc tttccatcat agttgacgaa gacaacacca catccgacat tgatttcttc
    33661 tcccagcgta gcatcaccaa cataagtcaa gtgaccgact ttagttcctt tgccgatttg
    33721 agcatttttc acttccacaa agtttccgat atggacgttt tcgccaattt cagcttttgg
    33781 tcgtaaatgt gcataaggtc ccacatctgc atgtttcttg acatggctgc tttcaatgac
    33841 tgagttttca atgacgacgt gatcctcaat cctactgtct acgatttttg aatgtgaccc
    33901 gatgacacaa tcagaaccaa tgactgtatt tccttggatt tgtacacctg cttcgatcaa
    33961 tgtgtctgga ccgatttcta cgcctgcatc gatgtatgtc gttgctgaat cgacaaagct
    34021 tacgccattg accatatgca tctggttaat tcgctttctc atgatctcat ttgctttagc
    34081 taacgcgatc cgatcattga ctcccattga ttcttcaaaa tcatccgttt gataagcagc
    34141 aactgtatgg ccttcttctt ttaagatttc gatgatatct gttagatagt attcaccttg
    34201 agcattgttt gttccaactt tgcttaacgc atcgaacaaa gcttcgttgt caaaacagta
    34261 agttcctgta ttgatttctt gtacaagagc ttcttctggt gttgcatctt tttgttccac
    34321 gattttttct acgatcccaa tatggtcacg gatgattcgt ccatatcctg ttgggttttc
    34381 tgcttgtgcc gtcaagatcg ttgcacttgc attttttcct tggtgatatt caaaaagatt
    34441 gttcagcgtt tcagtagtca ataatggtgt atcgccactg ataactaacg ttgttccttt
    34501 ttttccttcc aagaaagaag cagcttgcaa aacagcatgt cctgttccca attgttctgc
    34561 ttgtaacgca tatttactgc gttctcctag ttgtgcttta acttgttctg ctccgtgtcc
    34621 aacgatcgta atcacttcat ctggtttcgt ttcactgacc cgattaatga tatgttctac
    34681 cataggttta ccagaaacgg gatgtaaaac tttatataat tttgacttca tgcgggtacc
    34741 tttccctgcc gccaaaataa ttgcataacg tgcgtccaac tgcgtcactc ctaaatatag
    34801 tttgctgatt tcagtttatc ccagtatacc cattttttca acaaaacaag tgattttctt
    34861 ttatcccagc gttttctgga taaaaaaata gatagaccat aacgggccta tctatttggt
    34921 aaccggtgct ctcctgccaa ctcctcggtc acaagtcaca ctactcataa aacatgaaaa
    34981 gctgtttttt gaaatcgttt cttgtgactt agagttaaac ggtaggctta caacctcttc
    35041 attaaacgtt tttcaatcag ctgaccttcg gtattagctc aataaacggc aaaatatgag
    35101 gagcaatttt tccgtttatt gagctaatac tcaggacagg acgctgattt cacaacctct
    35161 agataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg aaaaaatgtg
    35221 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcggcttt ttcacaacct
    35281 cttattctga aaaataattt cctggcacaa ccgtaatatt tttcgtttga gtatctacat
    35341 ctttgacata aagcagtgac gtataatcat cgatggcgcg atgaccgttg aattttgatt
    35401 cggcaaatac agtgatgcca acaagttctg attcgaattc ttcaatcaag ctcttcattc
    35461 cgttgactgt tccgccgcct ttcatgaaat catccactac aaggacacgt gaaccacgtt
    35521 tcaaacttct cttagacagt tccatctttt caattctttc agaagatcca gacacatagt
    35581 tgacacttac tgttgatcct tctgttattt ttgagtcccg gcgtacaata acgaatggcg
    35641 cattcagata ataggacaca gcttgggcaa taggtacgcc ttttgtggct acagtcatta
    35701 cagcatcgat tttttcacca aggtatttag aagcaatgat tcttccaact tgacgcagca
    35761 gttcaggttc tcctaacaaa tcagagagat aaacatatcc tcctggaagc agacggtctt
    35821 gttctgatag acgttctgct agagattcaa tatatttttt cgcctcttca tatgaaattt
    35881 cagggatgaa caatacacca cctgcagcac ccgggattgt ttccaaggtt ccataccctc
    35941 gttctttaaa tgttttcttg atgattccca aatcttcact gatagaagat ttagcagatt
    36001 cataacgttc ggcaaagctt gttaaggaaa tcaattcatg cgggtgattc aataaatact
    36061 gtgtcatgtc aactagacgt tcactgcgac gtactttcac ctattccaac tccttttaag
    36121 aaattttatc gttttacatt atataagttt ttcttcaaaa attcgagcat tttatgaaaa
    36181 aataagaaaa tagttcggta tttattaaaa taaaaagaag cctataaaaa atttttcgtt
    36241 tttataaggc ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt
    36301 cgtttgatga tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct
    36361 ggaggtgtat ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca
    36421 caaacactga tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg
    36481 ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata
    36541 gccacaccag taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg
    36601 tgggcggtat cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat
    36661 ataccgatgg caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg
    36721 atcgaaccaa acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc
    36781 aatactaacg ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag
    36841 gttcggtaga tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg
    36901 gtcgtaagtg taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca
    36961 tgagaaagcg tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc
    37021 ggggcgattc cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa
    37081 agcaacgcca ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt
    37141 tgatatcttc atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat
    37201 gtcggagaag acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat
    37261 caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt
    37321 gtccgccaga caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta
    37381 aagcacgctg tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg
    37441 gatatcttcc tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg
    37501 caacttgttg aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa
    37561 tctttatcat tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact
    37621 tggctgctcc attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga
    37681 catcttctag tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac
    37741 gcaccttaca acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt
    37801 ttttccatga cagaaatata attttcgcct gctttgattt gttcattagt caagctttct
    37861 aaaggattca acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc
    37921 gaagaggcat tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt
    37981 agctcagcta agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact
    38041 tgttttaagt cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg
    38101 cggtttgttg catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg
    38161 atataagcat cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat
    38221 tcttcagcaa tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga
    38281 tcgtattcat gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc
    38341 gattctaaac ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact
    38401 gaatccttta acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct
    38461 gcatcggaaa tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca
    38521 gcagggatca aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac
    38581 atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta
    38641 tttccacatg ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt
    38701 ttcatttata tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact
    38761 aatgtatcaa aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg
    38821 tatattcttg attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta
    38881 caataaaaaa ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact
    38941 tcttatattt tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc
    39001 attatatact ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct
    39061 tccactcatc aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat
    39121 gactggatgc cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg
    39181 ctgataatct tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc
    39241 ttgcgtatct acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa
    39301 cacaacccag cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac
    39361 aaatgccgta cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc
    39421 tgccagttct tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc
    39481 tgcacagtct gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa
    39541 gatcttgaca ccggttttga tattgtattt attttttagc aataaagcag cttgataaac
    39601 attgttccgt tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc
    39661 tgttatttct tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa
    39721 ctcatgatac ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg
    39781 cgctttttca atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct
    39841 atcataggga aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt
    39901 tcaacagctt tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa
    39961 ttctgtgagc acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact
    40021 gagtagctgc atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc
    40081 ttcccaatga tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa
    40141 attaccataa ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc
    40201 tccaacatta gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta
    40261 tacaataaac agattggcta acagatagtc agctaattcc tcagattctt taggtaagac
    40321 ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac
    40381 gactttttga tctttacgga caataccgac accgctagat aatacatatg atttatcaaa
    40441 tcgtcccaca gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt
    40501 tacagcttga tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt
    40561 catcaaacgt tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc
    40621 aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac
    40681 gtagctttct gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat
    40741 aaactgattc gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa
    40801 gcatttgttt cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta
    40861 ataaaaataa ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt
    40921 ttccaataaa cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc
    40981 agctaaatat tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc
    41041 tgagaaccac acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac
    41101 taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa
    41161 gctagcatca aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt
    41221 tctggataac cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa
    41281 cgtacgtgtg attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc
    41341 ctttttgact tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag
    41401 attcactgtc tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact
    41461 gattgcagac agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac
    41521 agcacttacg agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa
    41581 aaatcgattg attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg
    41641 tagaacataa taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac
    41701 agacaattga ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac
    41761 catcgtcagc attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga
    41821 aatatctatt ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg
    41881 tccaaaggat gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt
    41941 ctttctcttt agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag
    42001 ccagatccaa tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt
    42061 aagaaacgac catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc
    42121 taaaaccatt tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac
    42181 cacaccacca aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa
    42241 ctcttttctt tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc
    42301 atacaacaac atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat
    42361 gcggctttgt tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt
    42421 tgcgacttga tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag
    42481 actatgttcc tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc
    42541 ctaacttgta tacttttgtg tcatacccca atgttatctt atttatttta atttttccac
    42601 tttagacctg ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag
    42661 aggataacat gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg
    42721 gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg
    42781 aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag
    42841 ttgcatcaca atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat
    42901 tggaagaact aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat
    42961 cattaataga tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc
    43021 cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca
    43081 cggaggaaca gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt
    43141 tagcagaatc agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt
    43201 acatgctgat cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag
    43261 ccactgagat tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata
    43321 aaaatccatg ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg
    43381 tagttgcctt agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga
    43441 tcgagaaaga tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt
    43501 acaaaaaaag aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta
    43561 gcataatcag ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc
    43621 agttcttctt cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag
    43681 atttcttctg gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat
    43741 tctacaccgg cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt
    43801 tctaatgatt cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca
    43861 aggacagaga taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt
    43921 actactgggt tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct
    43981 tcaatctcat ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat
    44041 tggatatctt cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa
    44101 tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt
    44161 agatctggat gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga
    44221 atacgttcat ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg
    44281 attcgtccat cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga
    44341 tcgaatccgg aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc
    44401 agcgtctcaa gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca
    44461 tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt
    44521 tgaatcgcta atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt
    44581 tcagattctt ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg
    44641 atgacatctc gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga
    44701 tcttcaatca ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta
    44761 agtgcttctt ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga
    44821 ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt
    44881 tctctcagct cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac
    44941 aatgcttctt tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct
    45001 gatgattgtg caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa
    45061 ccaagaccga caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc
    45121 tccatactat ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac
    45181 tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg
    45241 tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta
    45301 aacgatggtt tttaaaataa atgacactag tttcatatag gtaaaccttt aaattgagaa
    45361 acttttctat taaattattc tttgtagatg aacagatatt tttattttat gtacccactc
    45421 gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt
    45481 acctactctt cagaccagcc gtttttgttt atcataattt ttattcttct actaaaggca
    45541 attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg
    45601 ctctaactag agcagaaact tctgccatca tttcaggatg attagccata tagattttag
    45661 cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat
    45721 caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac
    45781 catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt
    45841 tgatctttgt acggttacca acaatatctg taccttgttt caattgttct gcacgacgta
    45901 cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat
    45961 tcccaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg
    46021 ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc
    46081 cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg
    46141 aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac
    46201 cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt
    46261 gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt
    46321 tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat
    46381 atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa
    46441 gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag
    46501 agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat
    46561 catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct
    46621 tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc
    46681 attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga
    46741 tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa
    46801 tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg
    46861 tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca
    46921 cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac
    46981 cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag
    47041 caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag
    47101 tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt
    47161 atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt
    47221 gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt
    47281 tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga
    47341 ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag
    47401 aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat
    47461 taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag
    47521 caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat
    47581 tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt
    47641 ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat
    47701 cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag
    47761 caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga
    47821 tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta
    47881 gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat
    47941 agtatacgct gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa
    48001 atcttgtcac agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata
    48061 agtcacaata tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga
    48121 gttagacggc aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac
    48181 accgctgttt ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata
    48241 aagcatgatt tgatctactg gaataccgat caggctgaat gggatattat tgataaacag
    48301 caagataata gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat
    48361 gacttctcca tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac
    48421 aatgatcgct actacccaac caggggcctt tccttggtca accaacatga taaaagcagt
    48481 cataactagc attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc
    48541 tcgcgcacgg gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac
    48601 tgcacctact agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc
    48661 taaaggtaaa cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa
    48721 tttatttggt aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg
    48781 aagtttccac cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt
    48841 gaaattgatc tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc
    48901 ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt
    48961 gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta actggtacgg
    49021 attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg
    49081 ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa
    49141 cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg
    49201 ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg
    49261 tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact
    49321 ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa
    49381 gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc
    49441 ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc
    49501 aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt
    49561 caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga
    49621 ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt
    49681 aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt
    49741 gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc
    49801 gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc
    49861 tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat
    49921 gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc
    49981 acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta
    50041 aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag
    50101 ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc
    50161 aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    50221 taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt
    50281 gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg
    50341 gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    50401 cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat
    50461 ataagttgat taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa
    50521 ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat
    50581 ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac tcgctgaagg
    50641 cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg atcgaatcga
    50701 tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta aattgattgg
    50761 caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc ttcttcagca
    50821 aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg atttctttga
    50881 ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca aagtgaaatt
    50941 cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga ttgtacatcg
    51001 ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg attgctgttt
    51061 tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta ataccaagta
    51121 caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt tcttttgtgt
    51181 tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct tcttggtttt
    51241 cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat aaaaccatat
    51301 tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg attttgtcga
    51361 tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac aagttattta
    51421 gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt tcctgaccga
    51481 tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt acaaaatcga
    51541 tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag ctggttttcg
    51601 taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga aagacatcac
    51661 catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct ggaactttca
    51721 ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa ttcgtactga
    51781 acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc aaaccgtttg
    51841 gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta ttcatttgca
    51901 tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac ttgttgtatc
    51961 tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa ttttgtttcc
    52021 ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc tagagaaagc
    52081 agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt tatttcacca
    52141 tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg gttcttttca
    52201 tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact agaggcataa
    52261 taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg aaaaccgcca
    52321 aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata cacatttgtc
    52381 tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg ttccttcacg
    52441 atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg cgactcaaat
    52501 ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac aacaaaaatg
    52561 tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact agcagtgatc
    52621 ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct gttttcaaaa
    52681 taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa actttccaaa
    52741 tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg atgattttta
    52801 atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga
    52861 tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg taaatttcct
    52921 ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg cgcacttaac
    52981 tttgtctgat ccgaataatt caaactattt gtttccaaca tgctgctgag aagtgaacgt
    53041 ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg gatcgttttg
    53101 tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata caccatattt
    53161 atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa cctgcctttt
    53221 ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt ttctaaatga
    53281 tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac ggctaaaagc
    53341 gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa agaagggagg
    53401 gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct tggatcttgc
    53461 tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt
    53521 gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg atttagaagg
    53581 agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt
    53641 tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag ttgtcaaagc
    53701 ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg aagaaactcc
    53761 tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga
    53821 caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat aaagaatcta
    53881 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    53941 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    54001 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    54061 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    54121 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    54181 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    54241 agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    54301 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    54361 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    54421 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    54481 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    54541 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    54601 aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    54661 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    54721 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca taaaaaaaaa
    54781 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    54841 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    54901 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    54961 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    55021 aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    55081 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    55141 gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt ataataactt
    55201 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    55261 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    55321 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    55381 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    55441 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    55501 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    55561 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    55621 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    55681 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    55741 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    55801 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    55861 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    55921 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    55981 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    56041 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    56101 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    56161 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    56221 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    56281 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    56341 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    56401 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    56461 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    56521 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    56581 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    56641 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    56701 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    56761 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    56821 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    56881 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    56941 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    57001 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    57061 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    57121 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    57181 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    57241 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    57301 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    57361 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    57421 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    57481 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    57541 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    57601 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    57661 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    57721 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    57781 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    57841 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    57901 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    57961 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    58021 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    58081 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    58141 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    58201 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    58261 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    58321 catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg ctttcgtatc
    58381 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    58441 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    58501 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    58561 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    58621 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    58681 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    58741 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    58801 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    58861 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    58921 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    58981 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    59041 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    59101 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    59161 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    59221 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    59281 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    59341 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    59401 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    59461 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    59521 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    59581 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    59641 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    59701 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    59761 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    59821 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    59881 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    59941 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    60001 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    60061 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    60121 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    60181 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    60241 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    60301 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    60361 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    60421 cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    60481 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    60541 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    60601 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    60661 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    60721 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    60781 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    60841 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    60901 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    60961 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    61021 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    61081 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    61141 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    61201 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    61261 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    61321 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    61381 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    61441 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    61501 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    61561 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    61621 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    61681 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    61741 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    61801 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    61861 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    61921 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    61981 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    62041 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    62101 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    62161 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    62221 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    62281 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    62341 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    62401 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    62461 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    62521 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    62581 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    62641 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    62701 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    62761 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    62821 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    62881 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    62941 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    63001 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    63061 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cggttttgag
    63121 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    63181 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    63241 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    63301 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    63361 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    63421 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    63481 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    63541 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    63601 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    63661 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    63721 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    63781 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    63841 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    63901 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    63961 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    64021 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    64081 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    64141 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    64201 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    64261 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    64321 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    64381 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    64441 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    64501 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    64561 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    64621 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    64681 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    64741 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    64801 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    64861 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    64921 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    64981 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    65041 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    65101 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    65161 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    65221 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    65281 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    65341 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    65401 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    65461 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    65521 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    65581 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    65641 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca
    65701 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    65761 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    65821 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    65881 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    65941 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    66001 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    66061 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    66121 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    66181 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    66241 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    66301 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    66361 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    66421 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    66481 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    66541 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    66601 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    66661 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    66721 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    66781 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    66841 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    66901 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    66961 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    67021 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    67081 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    67141 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    67201 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    67261 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    67321 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    67381 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    67441 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    67501 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    67561 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    67621 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    67681 cagtctcaca acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa
    67741 aaacggtaaa atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac
    67801 gctgatttta caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa
    67861 aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac
    67921 gctgatttta caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    67981 caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag
    68041 gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg
    68101 gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata
    68161 gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa
    68221 aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat
    68281 agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct
    68341 gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa
    68401 taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc
    68461 aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc
    68521 taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa
    68581 attatataac aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac
    68641 accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag
    68701 ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc
    68761 actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc
    68821 atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc
    68881 agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc
    68941 gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat
    69001 caatgttttt ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc
    69061 agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg
    69121 tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca
    69181 ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg
    69241 tatatctatt tgattctcca atatttccat ccctttctct ctattagcat catacatgat
    69301 ttattttaca tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag
    69361 aaaataagac gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc
    69421 ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag
    69481 cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg
    69541 ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg
    69601 ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc
    69661 tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca
    69721 ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct
    69781 cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt
    69841 gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt
    69901 aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa
    69961 gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg
    70021 atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt
    70081 tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa
    70141 agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca
    70201 ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa
    70261 gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc
    70321 ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg
    70381 aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat
    70441 aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa
    70501 cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc
    70561 tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt
    70621 tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc
    70681 atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat
    70741 cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt
    70801 aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt
    70861 cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga
    70921 aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc
    70981 cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt
    71041 gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt
    71101 tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa
    71161 ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg
    71221 aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct
    71281 ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga
    71341 agacaaccga tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt
    71401 ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat
    71461 cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact
    71521 ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag
    71581 ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag
    71641 ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag
    71701 catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc
    71761 gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa
    71821 atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc
    71881 tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga
    71941 tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga
    72001 atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc
    72061 taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa
    72121 aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga
    72181 aataactatg actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga
    72241 aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta
    72301 gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt
    72361 ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa
    72421 accttttatt cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct
    72481 tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt atccaaacca
    72541 aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga
    72601 tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg
    72661 ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct
    72721 ctgacttctt ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat
    72781 tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc
    72841 gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca
    72901 aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc
    72961 agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca
    73021 gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt
    73081 tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc
    73141 tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg
    73201 cttgattttc ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg
    73261 atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc
    73321 tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt
    73381 cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt caaagatctg
    73441 gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga
    73501 cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata
    73561 aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag
    73621 tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc
    73681 taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa
    73741 ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa
    73801 attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt
    73861 ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa
    73921 acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac
    73981 tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag
    74041 ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt
    74101 aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca
    74161 accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact
    74221 gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc
    74281 ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca atgtatataa
    74341 atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt
    74401 atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg
    74461 cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct
    74521 tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt
    74581 tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt
    74641 aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc cattatagct
    74701 tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc
    74761 actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa
    74821 actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg
    74881 agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg
    74941 tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt
    75001 ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg
    75061 ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc
    75121 gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc
    75181 ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa
    75241 taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag
    75301 tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt
    75361 aataaatttg ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg
    75421 ccttaaattg atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc
    75481 gttatggatt atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg
    75541 tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat
    75601 tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat
    75661 tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct
    75721 tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa
    75781 tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt
    75841 ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca
    75901 agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct
    75961 gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct
    76021 tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa
    76081 gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct
    76141 ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca
    76201 atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct
    76261 tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg
    76321 attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca
    76381 ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct
    76441 ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt
    76501 ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct
    76561 tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc
    76621 cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca
    76681 tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac
    76741 ccagtattca gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca
    76801 aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc
    76861 attttgctcc ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta
    76921 taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg
    76981 agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat
    77041 atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa
    77101 gatttagaag aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg
    77161 acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa
    77221 acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc
    77281 gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct
    77341 gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc
    77401 ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt
    77461 cgactgccag cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag
    77521 caaacagtca tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg
    77581 tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc
    77641 ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca
    77701 ttagctctag atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat
    77761 ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct
    77821 gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc
    77881 gctttcagct ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa
    77941 gtttcttaat ttgttccttc cttttatttc tgataattcc agtaaattac tataattata
    78001 aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg
    78061 tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg
    78121 aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc
    78181 aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat
    78241 tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa
    78301 aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag
    78361 gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag
    78421 aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga
    78481 tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc
    78541 gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat
    78601 gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat
    78661 atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc
    78721 gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata
    78781 tccattcaga accattttca ctgaattatt caagactggt gaatcattta cgactgcttc
    78841 tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca
    78901 aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag
    78961 aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt
    79021 taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc
    79081 atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc
    79141 tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt
    79201 tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt
    79261 agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt
    79321 tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc
    79381 agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg
    79441 attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc
    79501 tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag
    79561 tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt
    79621 cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat
    79681 ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa
    79741 tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc
    79801 tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa
    79861 tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ttagcagcag cttttgcttc
    79921 attctttact ggtgtaacag aaccacttga attctcattt atgttcgttg cttggccatt
    79981 atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg
    80041 gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc
    80101 gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta
    80161 ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc
    80221 agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc
    80281 aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac
    80341 tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc
    80401 cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac
    80461 agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg caacacgacc
    80521 tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga
    80581 tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt
    80641 cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc
    80701 tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac
    80761 agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga
    80821 accattcact ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat tgattgcttt
    80881 agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac
    80941 aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga
    81001 taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg
    81061 ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg
    81121 gcgttctcct gccaactccg cggtaataag tccagccaaa cgtaacatat gaagagctat
    81181 tttttatttg actcttctta ttacttgatg cagaacagct tttttacaac ctctgataaa
    81241 cggtgttcaa aaaccagttt atcggtatga agtcaaattt atgcaaaaat gtggaaagcc
    81301 attttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata
    81361 cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc
    81421 aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg
    81481 atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca
    81541 aacgctgcat cacttgcttc aataatttct aactttttac ctgtcgcacg ctctaatgcc
    81601 ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca
    81661 ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt
    81721 tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt
    81781 aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta
    81841 atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct
    81901 gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc
    81961 ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct
    82021 ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca
    82081 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
    82141 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
    82201 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
    82261 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
    82321 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
    82381 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
    82441 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
    82501 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
    82561 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
    82621 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
    82681 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
    82741 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
    82801 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
    82861 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
    82921 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
    82981 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
    83041 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
    83101 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
    83161 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
    83221 aattcttcag gcgattctgc taaagcgatt acaacaagat acgttccttt tgggaatggg
    83281 atgattcggg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
    83341 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
    83401 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
    83461 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
    83521 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
    83581 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
    83641 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
    83701 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaactcata atctgaagaa
    83761 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
    83821 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
    83881 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
    83941 cgggctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
    84001 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
    84061 ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct
    84121 ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag
    84181 cgtggaaaag atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa
    84241 tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct
    84301 gtcactcttg atccatctag gagatcgcga ttagccaaag ccaatctacg gtttttgata
    84361 tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag
    84421 attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg
    84481 taatcgatca attggttcag atctttaagc atatttatct tcctccttgc ttgccttttt
    84541 atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta
    84601 gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt
    84661 attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag acacattcta
    84721 agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg
    84781 tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga
    84841 tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt
    84901 tttgtcagat aaataaaa
//
LOCUS       DANJLF010000005        82882 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000005 DANJLF010000000
VERSION     DANJLF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            27..467
                     /locus_tag="PWT25_000387"
     CDS             27..467
                     /locus_tag="PWT25_000387"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL1020499.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            595..1278
                     /locus_tag="PWT25_000388"
     CDS             595..1278
                     /locus_tag="PWT25_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL1020500.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1587..1660
                     /locus_tag="PWT25_000389"
     tRNA            1587..1660
                     /locus_tag="PWT25_000389"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1621..1623,aa:Arg,seq:tct)
     gene            1819..2178
                     /locus_tag="PWT25_000390"
     CDS             1819..2178
                     /locus_tag="PWT25_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL1020501.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2301..3932
                     /locus_tag="PWT25_000391"
     CDS             2301..3932
                     /locus_tag="PWT25_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL1020502.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3994..4635)
                     /locus_tag="PWT25_000392"
     CDS             complement(3994..4635)
                     /locus_tag="PWT25_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL1020503.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4821..4897
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4917..5225
                     /gene="rplU"
                     /locus_tag="PWT25_000393"
     CDS             4917..5225
                     /gene="rplU"
                     /locus_tag="PWT25_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL1020504.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5252..5596
                     /locus_tag="PWT25_000394"
     CDS             5252..5596
                     /locus_tag="PWT25_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL1020505.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5609..5902
                     /gene="rpmA"
                     /locus_tag="PWT25_000395"
     CDS             5609..5902
                     /gene="rpmA"
                     /locus_tag="PWT25_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL1020506.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6336..9143
                     /locus_tag="PWT25_000396"
     CDS             6336..9143
                     /locus_tag="PWT25_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1020507.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9334..9825
                     /locus_tag="PWT25_000397"
     CDS             9334..9825
                     /locus_tag="PWT25_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1020508.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9928..10917
                     /gene="manX"
                     /locus_tag="PWT25_000398"
     CDS             9928..10917
                     /gene="manX"
                     /locus_tag="PWT25_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL1020509.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10950..11753
                     /locus_tag="PWT25_000399"
     CDS             10950..11753
                     /locus_tag="PWT25_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL1020510.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11774..12688
                     /locus_tag="PWT25_000400"
     CDS             11774..12688
                     /locus_tag="PWT25_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL1020511.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12771..13145
                     /locus_tag="PWT25_000401"
     CDS             12771..13145
                     /locus_tag="PWT25_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL1020512.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13284..13529
                     /locus_tag="PWT25_000402"
     CDS             13284..13529
                     /locus_tag="PWT25_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1020513.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13701..14765
                     /locus_tag="PWT25_000403"
     CDS             13701..14765
                     /locus_tag="PWT25_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL1020514.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15011..15436
                     /locus_tag="PWT25_000404"
     CDS             15011..15436
                     /locus_tag="PWT25_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1020515.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15429..15878
                     /gene="nusB"
                     /locus_tag="PWT25_000405"
     CDS             15429..15878
                     /gene="nusB"
                     /locus_tag="PWT25_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL1020516.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15975..16829
                     /locus_tag="PWT25_000406"
     CDS             15975..16829
                     /locus_tag="PWT25_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL1020517.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16830..18167
                     /locus_tag="PWT25_000407"
     CDS             16830..18167
                     /locus_tag="PWT25_000407"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL1020518.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18172..18396
                     /locus_tag="PWT25_000408"
     CDS             18172..18396
                     /locus_tag="PWT25_000408"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL1020519.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18398..19288
                     /locus_tag="PWT25_000409"
     CDS             18398..19288
                     /locus_tag="PWT25_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL1020520.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19297..20115
                     /locus_tag="PWT25_000410"
     CDS             19297..20115
                     /locus_tag="PWT25_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL1020521.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20222..20671
                     /locus_tag="PWT25_000411"
     CDS             20222..20671
                     /locus_tag="PWT25_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL1020522.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20689..22371
                     /gene="recN"
                     /locus_tag="PWT25_000412"
     CDS             20689..22371
                     /gene="recN"
                     /locus_tag="PWT25_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL1020523.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22426..22542)
                     /locus_tag="PWT25_000413"
     CDS             complement(22426..22542)
                     /locus_tag="PWT25_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL1020524.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22615..23571)
                     /locus_tag="PWT25_000414"
     CDS             complement(22615..23571)
                     /locus_tag="PWT25_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL1020525.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23742..24110
                     /locus_tag="PWT25_000415"
     CDS             23742..24110
                     /locus_tag="PWT25_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL1020526.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24280..24711
                     /gene="mraZ"
                     /locus_tag="PWT25_000416"
     CDS             24280..24711
                     /gene="mraZ"
                     /locus_tag="PWT25_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL1020527.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24732..25691
                     /gene="rsmH"
                     /locus_tag="PWT25_000417"
     CDS             24732..25691
                     /gene="rsmH"
                     /locus_tag="PWT25_000417"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL1020528.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25696..26097
                     /gene="ftsL"
                     /locus_tag="PWT25_000418"
     CDS             25696..26097
                     /gene="ftsL"
                     /locus_tag="PWT25_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL1020529.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26098..28290
                     /locus_tag="PWT25_000419"
     CDS             26098..28290
                     /locus_tag="PWT25_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL1020530.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28316..29278
                     /locus_tag="PWT25_000420"
     CDS             28316..29278
                     /locus_tag="PWT25_000420"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL1020531.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29292..30653
                     /locus_tag="PWT25_000421"
     CDS             29292..30653
                     /locus_tag="PWT25_000421"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL1020532.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30679..31767
                     /gene="murG"
                     /locus_tag="PWT25_000422"
     CDS             30679..31767
                     /gene="murG"
                     /locus_tag="PWT25_000422"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL1020533.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31782..33002
                     /locus_tag="PWT25_000423"
     CDS             31782..33002
                     /locus_tag="PWT25_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL1020534.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33158..34483
                     /gene="ftsA"
                     /locus_tag="PWT25_000424"
     CDS             33158..34483
                     /gene="ftsA"
                     /locus_tag="PWT25_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL1020535.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34506..35747
                     /gene="ftsZ"
                     /locus_tag="PWT25_000425"
     CDS             34506..35747
                     /gene="ftsZ"
                     /locus_tag="PWT25_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL1020536.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35764..36441
                     /locus_tag="PWT25_000426"
     CDS             35764..36441
                     /locus_tag="PWT25_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL1020537.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36455..37054
                     /locus_tag="PWT25_000427"
     CDS             36455..37054
                     /locus_tag="PWT25_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL1020538.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37069..37356
                     /locus_tag="PWT25_000428"
     CDS             37069..37356
                     /locus_tag="PWT25_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL1020539.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37405..38187
                     /locus_tag="PWT25_000429"
     CDS             37405..38187
                     /locus_tag="PWT25_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL1020540.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38208..38915
                     /locus_tag="PWT25_000430"
     CDS             38208..38915
                     /locus_tag="PWT25_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL1020541.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39177..41975
                     /gene="ileS"
                     /locus_tag="PWT25_000431"
     CDS             39177..41975
                     /gene="ileS"
                     /locus_tag="PWT25_000431"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL1020542.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42024..43550)
                     /locus_tag="PWT25_000432"
     CDS             complement(42024..43550)
                     /locus_tag="PWT25_000432"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL1020543.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43565..44212)
                     /locus_tag="PWT25_000433"
     CDS             complement(43565..44212)
                     /locus_tag="PWT25_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL1020544.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44396..44725)
                     /locus_tag="PWT25_000434"
     CDS             complement(44396..44725)
                     /locus_tag="PWT25_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL1020545.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44902..45630
                     /gene="sfsA"
                     /locus_tag="PWT25_000435"
     CDS             44902..45630
                     /gene="sfsA"
                     /locus_tag="PWT25_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL1020546.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45646..46659
                     /locus_tag="PWT25_000436"
     CDS             45646..46659
                     /locus_tag="PWT25_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1020547.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46659..47936
                     /locus_tag="PWT25_000437"
     CDS             46659..47936
                     /locus_tag="PWT25_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1020548.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            47999..50701
                     /locus_tag="PWT25_000438"
     CDS             47999..50701
                     /locus_tag="PWT25_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1020549.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50853..51170
                     /locus_tag="PWT25_000439"
     CDS             50853..51170
                     /locus_tag="PWT25_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1020550.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51200..51520
                     /locus_tag="PWT25_000440"
     CDS             51200..51520
                     /locus_tag="PWT25_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL1020551.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51628..53088
                     /locus_tag="PWT25_000441"
     CDS             51628..53088
                     /locus_tag="PWT25_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1020552.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53156..53377
                     /locus_tag="PWT25_000442"
     CDS             53156..53377
                     /locus_tag="PWT25_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020553.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53408..53590
                     /locus_tag="PWT25_000443"
     CDS             53408..53590
                     /locus_tag="PWT25_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL1020554.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53590..54003
                     /locus_tag="PWT25_000444"
     CDS             53590..54003
                     /locus_tag="PWT25_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL1020555.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54126..55307
                     /locus_tag="PWT25_000445"
     CDS             54126..55307
                     /locus_tag="PWT25_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1020556.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55378..55542)
                     /locus_tag="PWT25_000446"
     CDS             complement(55378..55542)
                     /locus_tag="PWT25_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020557.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55696..55769
                     /locus_tag="PWT25_000447"
     tRNA            55696..55769
                     /locus_tag="PWT25_000447"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55730..55732,aa:Arg,seq:cct)
     gene            complement(55806..56006)
                     /locus_tag="PWT25_000448"
     CDS             complement(55806..56006)
                     /locus_tag="PWT25_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1020558.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56311..57543)
                     /locus_tag="PWT25_000449"
     CDS             complement(56311..57543)
                     /locus_tag="PWT25_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL1020559.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57797..58366
                     /locus_tag="PWT25_000450"
     CDS             57797..58366
                     /locus_tag="PWT25_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1020560.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58544..58984)
                     /locus_tag="PWT25_000451"
     CDS             complement(58544..58984)
                     /locus_tag="PWT25_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL1020561.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59142..59906
                     /gene="map"
                     /locus_tag="PWT25_000452"
     CDS             59142..59906
                     /gene="map"
                     /locus_tag="PWT25_000452"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL1020562.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            59938..60861
                     /locus_tag="PWT25_000453"
     CDS             59938..60861
                     /locus_tag="PWT25_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL1020563.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            60937..62076
                     /locus_tag="PWT25_000454"
     CDS             60937..62076
                     /locus_tag="PWT25_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL1020564.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62069..62869
                     /locus_tag="PWT25_000455"
     CDS             62069..62869
                     /locus_tag="PWT25_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1020565.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62869..63696
                     /locus_tag="PWT25_000456"
     CDS             62869..63696
                     /locus_tag="PWT25_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1020566.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63674..64408
                     /locus_tag="PWT25_000457"
     CDS             63674..64408
                     /locus_tag="PWT25_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1020567.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64508..65374
                     /gene="rfbA"
                     /locus_tag="PWT25_000458"
     CDS             64508..65374
                     /gene="rfbA"
                     /locus_tag="PWT25_000458"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL1020568.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65388..65960
                     /gene="rfbC"
                     /locus_tag="PWT25_000459"
     CDS             65388..65960
                     /gene="rfbC"
                     /locus_tag="PWT25_000459"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL1020569.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            65982..67010
                     /gene="rfbB"
                     /locus_tag="PWT25_000460"
     CDS             65982..67010
                     /gene="rfbB"
                     /locus_tag="PWT25_000460"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL1020570.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67108..67959
                     /gene="rfbD"
                     /locus_tag="PWT25_000461"
     CDS             67108..67959
                     /gene="rfbD"
                     /locus_tag="PWT25_000461"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL1020571.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            67994..70027
                     /locus_tag="PWT25_000462"
     CDS             67994..70027
                     /locus_tag="PWT25_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020572.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70071..71351
                     /locus_tag="PWT25_000463"
     CDS             70071..71351
                     /locus_tag="PWT25_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL1020573.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71561..72367
                     /locus_tag="PWT25_000464"
     CDS             71561..72367
                     /locus_tag="PWT25_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020574.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72379..73599
                     /locus_tag="PWT25_000465"
     CDS             72379..73599
                     /locus_tag="PWT25_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020575.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73589..75175
                     /locus_tag="PWT25_000466"
     CDS             73589..75175
                     /locus_tag="PWT25_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1020576.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75214..77352
                     /locus_tag="PWT25_000467"
     CDS             75214..77352
                     /locus_tag="PWT25_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1020577.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77720..78703)
                     /locus_tag="PWT25_000468"
     CDS             complement(77720..78703)
                     /locus_tag="PWT25_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL1020578.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79081..80472
                     /locus_tag="PWT25_000469"
     CDS             79081..80472
                     /locus_tag="PWT25_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL1020579.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80488..81528
                     /locus_tag="PWT25_000470"
     CDS             80488..81528
                     /locus_tag="PWT25_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1020580.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81547..82632
                     /locus_tag="PWT25_000471"
     CDS             81547..82632
                     /locus_tag="PWT25_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL1020581.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            82665..>82882
                     /locus_tag="PWT25_000472"
     CDS             82665..>82882
                     /locus_tag="PWT25_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL1020582.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQA"
BASE COUNT        27100 a        13947 c        17545 g        24290 t
ORIGIN      
        1 ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata
       61 ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac
      121 cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa
      181 aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa
      241 aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta
      301 gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga
      361 ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct
      421 ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac
      481 ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat
      541 gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc
      601 aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag
      661 caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta
      721 ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct
      781 gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca
      841 atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct
      901 aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca
      961 gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa
     1021 tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg
     1081 atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt
     1141 ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc
     1201 gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca
     1261 gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg
     1321 gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga
     1381 aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct
     1441 tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat
     1501 aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta
     1561 taattattga tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct
     1621 tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact
     1681 gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa
     1741 taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc
     1801 agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt
     1861 gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg
     1921 gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac
     1981 caaggagtgt acaatggact attaggagtg ggactacttt atggagctta tctttcttcc
     2041 gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc attatatggc
     2101 agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct gttggcattg
     2161 ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga aactttgtga
     2221 aaaatattac tttatctcat gaaatatgat attctaatga atggtatttt agaaaatttc
     2281 atgaaaaagg agttagatag gtgttcaaaa attatgtttc ctcactaaaa aaagaatttt
     2341 caggttatga tctttcaaga tttcagaaag atcttttggc tgggatcaca gtagctgctg
     2401 tagcattgcc tttagccttg gcttttgggg tatcaagcgg agctgatgcg gccgcaggtt
     2461 tgattacggc tatcattgcc ggtatcgtta ttggcggttt atcaggcggt ttttatcaaa
     2521 tatcaggtcc gacaggtgcg atggcagcta tcttgatgtc tattgcggca acaaagggaa
     2581 tgcaaggtgt attgcttgct acttttttag cgggtctatt tttacttgca gcaggacttt
     2641 tccgattagg aacactgact tcttttatcc cagcacccgt aattacgggg tttacttcag
     2701 gtattgctat cattattgca ttaggtcaga tcgataattt atttggtgtt cactcggaag
     2761 gtacaaacgt tattgaaaaa ctaagtagtt atcaaacgat aggatttccg ataaacacag
     2821 cctctttgat catgggaggg ctagtcattt tagggatgtt tatctatcca aaaaaatggg
     2881 cgaaacgaat gcctagttcg ctgctggcta tcattttggc aacagcagtt atgctattgt
     2941 tccatttacc agtagcaact gtgggtaaga ttccacaaac attgatcagt agtaatcgtt
     3001 tgaatatggg agattttagc ttttctgcct tgcaacaagt ggcagttcca gctattagca
     3061 ttgctttatt aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc cgaatggcaa
     3121 ataagccctt agatagcaat caagaattaa tggcgcaagg aattggaaat ctgttgcttc
     3181 ctttctttgg cgggattcct gctactgcgg ccatcgcccg tacaagcgtg gccatcaaat
     3241 ctggtgcaca aactagatta gctggtatga tacatgcagt tgtcttactg ctctccatgc
     3301 ttgtttttgc gccagtgatg tcacagattc caatgccagc acttgcaggt gttttgattg
     3361 tgacggcttg gcgtatgaat gaatgggaaa cgatcaagga gctatttact aataaatatt
     3421 ggtcagcagt tctattgttt atccttacga tgatttgtac agttatcttt gatttaagta
     3481 ttgctatcgt gatcggggtg atcggcggct gtgtattttt tgttgtcaaa agcgctgcta
     3541 tcacgatttc attagaaacg atcgattggc aaagaatggg gctgccagaa acggaaaagc
     3601 tgaataactg gacagtagtg tatattagtg gtccactgtt ttttatgtct tcagaaaaac
     3661 tcaaaaataa attggaagaa ctaaatcatc acgatggtgt cattttctcc atgcgaggcg
     3721 taccaagtat tgatttgacg gcacgtgcag tttttgatga atttcaagaa aaagcagagc
     3781 aaagaggaca aacgattatc tatacttcct tgcagccaga agtagaaaaa caactttctc
     3841 acttgtggga acatacattc acgcatcaac atcaaacagt tgctcatgcg ttgacagctt
     3901 tacatgcgaa atatactgca attagccatt aaaacaatga aaaagaacag acaagactgt
     3961 aaactgaaga ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa gtgaagctag
     4021 tacttgccag tcttgatgga taacaggtac aagggagcga ttgtagaaaa tagctgctgg
     4081 atgaaaaagt ggaagaattc gatagttttt ttctgaccat tcgtaggcat ccttttgttc
     4141 tgttaattgt aagacaggcc ctgtatagag ttgaccatga tttttggtaa ccgtatattc
     4201 tttgccaagt aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt ttggctggac
     4261 cttcatcagt tcgtaatcca atattggtgc atgcgcaaat acttcggaac gagtaggtgt
     4321 ccgatttgga taaacagttt ctgactgttg tgtccgttta ttgatccgat gagtcacgcg
     4381 atacgggcgg ctccttacag cactggtgat atacacctct tctcgtgtta agccagttga
     4441 agaaagtgcc tgcatcaatt cttttccagc ttgaccgcta aaaggaacat gcgtatcaat
     4501 ctctgttttc cctggggctt ctcccacgag catcagttta ggatgaagag gtccttgccc
     4561 agcgataaat ccttctacag gacggccgtt cattcttaaa gaagcttctt gtatcagttc
     4621 ttttgggtat tccatttatg ctcctccttt ttcttttatt ataagaataa aagaaacaca
     4681 atgcgactat tttatttggt agaactgtga ggatagaaaa gcagaagtat tgcttgaatt
     4741 tttgttttac tttttgattt taaatttagt tgactaacgg ttgctgttct ggtaagctag
     4801 tactgttgta attgtggact ccacagctac aaccgctcag aacaagtaat aagcacagca
     4861 tatgctgtca cttcggatgg cgagtctaag tatatcagaa ggaggtgctt tatagcatgt
     4921 acgcaattat taaaactggt ggtaaacaag taaaagttga agtgggacaa gcaatctatg
     4981 ttgaaaaact tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt atcttagtag
     5041 gcggagaatc tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt gaaggtactg
     5101 ttgaaaaaca cggcaaacaa aagaaagtcg ttactttcaa atacaaacct aaaaaacatt
     5161 cacaccgtaa acaaggtcat cgtcaaccat atacaaaagt ggttatcgac gcgatcaacg
     5221 cttaatagcg tttgagaaag aaggcctata tatgatcaaa ggaacattta agcgaaatga
     5281 cgcaggtcag atcgtttcat tcactttgac cgggcatgct gatgctggtc cttatggaag
     5341 tgatatcgtt tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg ggattgcctc
     5401 attagccggt tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt atctttatac
     5461 cgaagtcact tcaggaatga cacaagaaca aaacaatatt gctgagattc tattagaaaa
     5521 tcttttatta ggattacaat cgatagaagc tgaaaactca gaatatatcc aaattaaaac
     5581 aattaatgat aaataggagg tgcagaccat gttgttatct atgaatctac aattatttgc
     5641 ccacaaaaaa ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa aacgtctagg
     5701 tgctaaacgt gctgatggac aaacagttac aggtggatca attctttacc gtcaacgcgg
     5761 tacaaaaatc tatccaggtg taaacgtggg tatcggtgga gacgacactt tatttgcaaa
     5821 agttgatggc gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag tgtctgttta
     5881 tccagtagct caagaagctt aattgaatct tatgattaaa gaggttgtga caagactttt
     5941 gtcacagcct tttttgttta taaaaataaa agttgtagtg gaggctacga agtttacttt
     6001 gtctttttac acttaatatt tttttgccaa tttggaatca ggatgttttg ataactatcg
     6061 ttttcaattg atggaagcta tattgtttgc tatcagtgaa ataattatat ggtaaacagt
     6121 gttctttttc ttgtttccat tataaaaatc gtatcgggat ttttcgttct tttaaattga
     6181 acatttctat ctatccgcgt gttatcaaca aaacaaaatc aggtaaatag ttgacgaaac
     6241 agaaataaat acatagtatt atgttttgaa gcctaagtac aatagtctat agaaaaggag
     6301 cgataccatt ggcaaaacgc attgaccgaa tttatatgta tgtcaaagaa aaaaccgctc
     6361 atttaacaaa ggcagaatat gatcaaggag taacaacaca ggaaattgct gaagtgttag
     6421 ggattcagcg aacaaatagc agtaaagatc tgaatcagct agtgcgcgaa ggaaaactga
     6481 taaagacaga cggacgacca gtacgttaca tatatcaaac actcgttacg cacgtaaagc
     6541 cagttacaaa acatgtagtc agttacaaag aaacgccggt ctctgaagaa aaaaatacac
     6601 tccaaataga tactaaggat atctttgcaa aaatcattgg agctaacggc agcatgaaaa
     6661 attcagtgga acaggcaaaa gctgcgattt tgtatccgcc aagaggatta aattgtttga
     6721 ttacaggacc taccggatca ggaaagactt attttgcaca cgcgatgtat cattttgcaa
     6781 aagcgcatca tgtaatagat gaggaaacgg aactgattgt tttcaattgt gctgattatg
     6841 ccaataaccc tgagcttttg atgagtcatt tatttgggta cgcaaaaggt gcttttacag
     6901 gcgcagatga agaaaaaatg gggattatcg atcaagcgga tggcggtatg ctgtttttgg
     6961 atgagattca ccggttgccg cctgaaggtc aagaaatgat cttttacttt atggatcatg
     7021 gaacttacag tcgtcttggg gaaacaacga aatcccatga agcaaatgta cggatcgtag
     7081 gtgcgaccac agaagaccca ggttcttctc tattagaaac ttttgttcgg cgtatcccaa
     7141 tcaatatcaa acttccggct tttgaaaaac gtcctgcaaa tgagaaagtc gatttagtaa
     7201 aaatcatgat tgcccacgaa gcaaaccgca cgcagcgaaa gatttctttg acagaagatg
     7261 tagcaaaagc attgatcggc agtgtgacct atggaaatat aggacaattg aagtcaaata
     7321 tccagttagt atgtgcacga ggatttctaa atcatatgca atcacccgaa atctcgatca
     7381 caatcgatga tttgacagaa gggattcgta gcggattgat tcaattagca agcaaccgca
     7441 gtgcaatggc agagctttct aaaatattgg agccaaagat aactgtagca ccaaatgata
     7501 cgatcatgaa aatccaatcg gactcctatg agttacctta taatctatat gatatcatcg
     7561 gagataaagc agctttactg aagtcggatg gtttggatca agaagcaatc aatcatttca
     7621 tttctacaga tatcaacatc catctgaagt ctttctataa agaccatggt ttttctttta
     7681 atgcagacag caaattagca gaatttgtag atccgaaaat catagaagtg accaatcaga
     7741 tttatgtaat ggtcaaaaga tctttaccat atgagtttca gcaaaacttt atttatgcta
     7801 tgagcttgca tatcagttct tttttgaaaa aaatcaactt aggagaagaa cggcatacga
     7861 atgataacat ccgtgagatg gcttttgatt ttccagatga atatgctgtt gcgaaagaag
     7921 tgcgtagtta tatcgaaacg tattttcaag tggagattcc agaaagtgaa gactattact
     7981 tgaccgttct actcgtctcg ttacgggcaa atcaagcaag cggcagaatc ggtgtagtcg
     8041 ttgcagctca cggaaacagc acagcaagca gtatggtcca agttgtccaa cagttgttag
     8101 atgcagagaa cgtacgagca gtagacatgc ctttggatat ggatccgaaa acagcattgg
     8161 accgaattga acgcaatgta caagaagtag atgaaggaag cggggtcata ctcttagtag
     8221 atatggggtc ccttgcgtcg tttaattcgc aaattcaaag agacactgga attccagtac
     8281 gtactgttga tatggtaaca acttctctag ttttggaaac tgtacgaaag gtctcagtat
     8341 tagggacaga tctagatatg ttgcatgaat cattgaaaaa ttttcgaggt tacgcagaaa
     8401 tatcttctgt agaatctgtt caggaagcaa aacctattca taagaaaaaa gctgttttgg
     8461 ctatctgcgc atctggcgaa ggtacagcac aaagaatcaa agaattgatc gaacgagcgg
     8521 taaacaagcg tcaagaaaca gaattagaag ttctagcttt gtcgattgtg gaagtgaaag
     8581 aagaactgcc agttatccaa aaagaatatc agatcatagc tactactggg attactgatt
     8641 tgaatatcca agccccattt attcctttag aacgttttat tgatcaaaat atcgaagtca
     8701 ttttggatca gttgctgatg gaatcagaat tggaggaaac tgaattcatc tccctagatg
     8761 aagagtcagc taaaaatacg tgtgttgagt ttatctctga taacttcatc tttattaatg
     8821 gtagtaagtt aatcgatcca atgtggcaat tttctacaca aatctctcag acgacgggaa
     8881 tcggcgatga agaatatgga ttcaaaatca acctagtgat gcacacagca ggaatgatcg
     8941 aacgtattat tcggaatgaa ccattgactg tggaagaaaa cgaattgaca aatacaacga
     9001 atgatcctct ttatagccaa ttggcagcct cagttgttct gttggaagat cagataaaag
     9061 taaaagtgcc gatagaagaa atgtattatc tgctacgtct agtccataat cagttagata
     9121 aaaaagaata cactgtgcca tgaatacaca aaaacgatac acttttatag tgtatcgttt
     9181 ttgtgtattg gttaagaatc ctttaaattc aattagtgta tcgtaaaaaa gttggcacgc
     9241 tttttgctta tagtagtgtg tgctaggagg tggttgttca cacagaaatg ataggggttt
     9301 ctgtgaagta gtgaggaaga aggagttttg cagatggata ttcgattggc acgtatagat
     9361 gatcgtttaa tccacgggca agtcgcaaca gtttggtcga agcaaacagg tgttgaacgt
     9421 atcattgtga tcagcgatgt agtcgctcaa gataaattga gaaagtttct actaaaagaa
     9481 gctgcaccgc cggggatcac agcaaatgta ataaccgtgc agaaaatgat tcaaatctat
     9541 catagtggat tattatcacg agcaaaagtg atgcttttat tcaccaatcc ccaagatgtt
     9601 gaagcggttg tccgctgcgg tgtacagttg aagtcgttga atatcggcgg tatgagtttt
     9661 actgaaggca aatcaatgat taccaatttt gtttcagtaa atcaacaaga tattgattcc
     9721 tttcgttact tgaacagtca agaagtggag ttagagattc gaaaagtacc tgctgacagg
     9781 aaaatccttt tgatggaact actagcgaaa atcaaaaagg tgtagcaaga atggttgttg
     9841 gaaaaggtca aataattctg ttgtaatggt tagcaattat taatattttt gaatttgtta
     9901 caacaaaagt aaataggagg tataaatatg gtagggatta tcctagcaag tcatggacaa
     9961 tttgctgaag gaatcttaca atccggttca atgatctttg gcgaacaaga aaacgtcaaa
    10021 gccgtgattt tgaaaccaag tgaaggtcca gatgacttga gagccaaatt ggaagaagcg
    10081 gttgcttctt ttgataatca agacgaagtg ttattcttag ttgatttatg gggcggaaca
    10141 ccgttcaatc aatcaaacac attattcgaa gagcacaaag ataaatgggc aatcgtcagc
    10201 ggtttgaatt taccaatgtt gattgaagcc tacgcatcac gcttttcaat ggaatctgct
    10261 catgaaattg cagcacacat cattgaaaca gcaaaagacg gtgtaaaagt aaaaccggaa
    10321 gaattagaac cggcagaagc accgaaagca gcagttgaag acgcacaacc taaaggagca
    10381 cttccagaag gaactgtggt cggagacgga aaaatcaaat atgtattagc acgtgttgac
    10441 tcacgtctgt tacatggaca agttgcgaca gcttggacaa aagcagtgca accaaaccgt
    10501 attatcgttg tttcagatgc agtatcaaag gatgatctgc gtaagagact aatcgaacaa
    10561 gctgctcctc caggtgtaaa agcaaatgtt attcctatta gcaaaatgat cgaagtagca
    10621 aaagaccctc gttttggaaa taccaaagca ttgcttttat ttgaaaatcc tgaagacgta
    10681 ttgacagctg ttgaaggtgg cgtagacatc aaagaattaa atgttgggtc aatggcgcat
    10741 tcggtcggta aagtcgttgt aagtaaagta ttgtctatgg gacaagaaga cgttgaagcg
    10801 tttgagaagc ttgaacaaaa aggtgtgaaa ttcgatgtac gtaaagtacc taatgattca
    10861 cgcgataaca tggatgatat tttgaaaaaa gcaaaagctg aattagcaaa agcgtaataa
    10921 ataatattaa aatttatagg aggcttatca tgtctattat ttcaataatt ttagtcgtat
    10981 tagttgcctt tctagctggt atggaaggta ttcttgatga atttcaattt catcaaccac
    11041 tagtggcatg tacattgatc ggtttagtta ccggtaacct cgaagctggt atcgtacttg
    11101 gcggtacgct tcagatgatc gctttaggtt gggcaaatat cggtgccgct gtagcaccag
    11161 atgctgcatt agcatcagta gcatcagcaa ttattttagt tttaggtgga caaggtgtta
    11221 aaggtgttcc ttctgcgatc gcgattgccg ttcctttggc tgttgcaggg ttgttcttaa
    11281 caatgatcgt acgtacaatc gctgttccaa tcgttcattt gatggatgcg gctgctgaag
    11341 aaggaaacat ccgtaaggtt gaaatgtggc acatcattgc tgtatgttta caaggggttc
    11401 gtatcgcggt cccagcagca gcgttgctat ttatcccagc acaaactgtg caatcattcc
    11461 ttgaatcaat gccagcatgg ttaacagatg gtatggctat cggtggggga atggtcgtag
    11521 ctgtaggtta cgctttggta atcaacatga tggctacaaa agaagtatgg ccattcttcg
    11581 ttattggttt cgttgtagct gcaatttctc aattaacatt gattgctcta ggtgctttag
    11641 gtgttgcatt agctcttatt tacttgaacc tttctaaaat gggtggttca tcaaataatg
    11701 gaggcggaag caactctggt gaccctttag gcgacatttt gaatgactat taatcttgaa
    11761 ggaggaaaat aaaatggcag aagaaaaaat caaattatca aaaaaagatc gtttagctgt
    11821 tgcgtggcgc tctaccttca ttcaaggttc atggaactac gaacgtatgc aaaacggtgg
    11881 ctgggcattt tctatgattc cagcaatcaa aaaattatac aaaacaaaag aagaacgttc
    11941 tgcagcatta aaacgtcact tggaattctt taatacacac ccatacattg cttctccaat
    12001 tttaggggtt acattggctc tggaagaaga acgtgcgaat ggcgcgcctg ttgatgatgt
    12061 agcgattcaa ggggttaaag taggtatgat gggtccttta gctggtgttg gtgacccagt
    12121 gttctggttt acagtacgtc caatgctagg tgcactaggt gcttcattag ctatgggagg
    12181 aaatatcctt ggaccaatca tcttcttctt aggttggaac attattcgtt gggcattcat
    12241 gtggtataca caagaatttg gctacaaagc tggttctaaa attactgatg acctttctgg
    12301 cggattgtta caagacgtaa caaaaggtgc ttcaatttta gggatgttcg tattagctgc
    12361 ccttgtacaa agatgggtat caatcaaatt cttaccagtt gtatcaactg taaaattaga
    12421 taaaggtgct tacatcgaat gggataaatt gccttcaggt ggtgaaggaa tcaagagtgc
    12481 atttgaacaa gttaataatg gcttagccct ttctcctgaa aaggtaacaa cattacaaga
    12541 caacttagac caattgattc ctggtttagc tgcattagga ttaacattgt tatgtatgtg
    12601 gttgcttaag aaaaaggttt ctccaatcgt tatcatcctt ggtttattcg tagtcggtgt
    12661 tgttggacac gtcatcggac ttttataatc cataaaaata tagctcgggc gttgtcccga
    12721 gcttttttta tatagtatac tgttcataaa caaatatctg gagggagaaa atggttcaat
    12781 caatcaatac aaaagtagac ttggtaattg atgctacttc gtttacgggt ctgacagatt
    12841 acgggcaaat catgataggt gataatggtt ttgagtttta ccatgcgcga gatagacgca
    12901 aatatatcca aattccatgg gaagaagtgg attatgtact agcatcagtc atgctaaagg
    12961 gaaaatggat tcctcgatat gccattcaaa cgaaaaaaaa tggtacatac acattttctt
    13021 ccaaagaacc taaaaaagtg cttcgtgcga tcagagaata tgtagatgaa gatagaatgg
    13081 tacaatcatt gagtttcttt gatgtagtga aacgtggtat tcgtagtatg tttaaacgca
    13141 agtaaaaaat aaagaaaact tctaagagga ggttttcttt atttttttac tttgcagaag
    13201 aaaatatgaa atagcaaaac caattatttg tcccctttaa atcccatatg ctaaaattga
    13261 ttagcaaaga aggaggttat attatgagtt ggttatggac attgattgtt ggagccgtga
    13321 tcggagcaat tgcaggtgcg atcaccagta aaggaaattc gatgggctgt gtaacgaata
    13381 tcattgctgg actagtcggt tcaagtattg gacaagcgat tttcggcgaa tggggaccaa
    13441 aagctgcagg tatggcactt attccatcta ttttaggcgc agtgattttg gtagcagttg
    13501 tctcattttt ctttggaaaa cgatcataag tttttacaaa atagtaaact aaaaggagtt
    13561 gcagcgagac tgtgactcct ttttggcttt atttatttaa aataaaaaga agttttccgg
    13621 aaatttttat ttaactgtcg tgtacgaaga aaagtttgct ataatggaaa aaagcgtgca
    13681 aagaaagagg gagtttcatt atgatgctac gtgtagaaaa attgcgcaaa aaaatgcaag
    13741 aagaaaattt ggattcattt ttggttacca gtccgtacaa tcttcgttac ttaacgaact
    13801 ttacaggtac gacaggatta gctgttatta ctttggaaaa agcatttttt attactgatt
    13861 ttcgttatac cgagcaggcg gcagcgcagg ctcaagggtt tgaaattatt aaaaatgtcg
    13921 gacctatttt tgatgaggta gcaaacttag tacgcaaaga agaactgcac tcattagctt
    13981 ttgaggaaac aacagtctca tttttagaat attcggtttt agaagaaatc atcgatgcgg
    14041 aattagtacc tgtttcacag atgatcgaag aactcagaga agtgaaagat gaagaagaaa
    14101 tcgctattat cgaaaaagca tgtcatattg cggatatggc atatgatcac atcctgaaaa
    14161 tgatccaacc gggcatgact gagattgaag tcgcaaatca actagacttt tatatgcgtt
    14221 ctcttggcgc ttcaggtgtt tcttttgata cgatcgttgc tagcggattg cgttcggcta
    14281 tgccgcatgg tgtagcaagc aaaaaaatca tcgaacaagg tgacttgatc acattggact
    14341 ttggttgcta ttacgaaggg tacgtttccg atatgacacg tacattcgct attggtgatc
    14401 ctggggaaaa attaaaagaa atttatcaaa tagtcttaga tgctcaatta gcagttattg
    14461 atgctgcaaa accaggaatg actggtgtcc aattagatgc agttgctaga gattatattt
    14521 caaaacatgg ttacggggaa gcatttggac attctacagg acatgggatc ggtttagaga
    14581 tccacgaagg accaaacgtt tcttctcgtg cagaaaagca atttgtacca ggaaatgtga
    14641 tcactgatga accagggatc tacttaccag gaatcggtgg agtacgtatt gaggatgact
    14701 tgttgatcac agaagatgga aatcgtgtgt tgactcgttc accaaaagaa ttgatcatct
    14761 tatagtttca tcaatagaag aagtaagcgt aaaaatcagt gttcacagac tttgttatag
    14821 aaaatcactc gatttaatga tacactatag aagaactttt aagctgtaag agataataaa
    14881 gttgggtgtt ttctccaatt catagcagtt tgcagctgat cggcggcagt tttcttacca
    14941 cgtgatcact atgccgcatc atagagaatt cggatgtatc tcgtgcttag gcaaagggag
    15001 gaataaataa atggcagatg aaaaaaattt gattttaaac gcaaaccaag atttaggaga
    15061 gatcgtgatc gctcctgaag tcatcgaagt aattatcggg attgcagctt ctaaagtaga
    15121 aggcgtatac ggaatgcgcg gaacttttgc cagcaatgtc actgaattat taggacgtgc
    15181 agctcacgga aaaggtgttt acttagcaaa cgaggaagat ggcttgaaag tagatcttta
    15241 ctgttatcta gaatatggtg tttcagttcc taaagtagca atggaaatgc aggaacgggt
    15301 cagccagcaa gtccttttca tgacggatat cgatttatct gaagtcaata tccatgtagt
    15361 agctgttgtt cctgaaaaat tagaacaacc aacaatcgaa gaactttttg aagacgaaga
    15421 ggaagagaat gaataaagaa ttatcaagac acgagattcg ggaaatggca ttgcaggctt
    15481 tatttccatt agacttcaat gcagacctaa caaaagaaga tgcgattttc aacgcaatcg
    15541 aattagacca ccgcgacatg atcaacgaag atgaatcaga gtttgttcct gtttatcttg
    15601 atacgctagt tggcggtgtc tgtgcaaaaa aagatgaatt ggataaagtg attgaaaaac
    15661 atttaaaaaa caattggcac atcaatcgga tctcaaaaat ggatttggtg atcttgcgta
    15721 tcgcgatttt tgaaatgact tatgtgacag atgtaccagc ggctgttgct ttaaatgaag
    15781 caatcgagtt ggcaaaaaca tttagtgatg accgttcacg taaattcgtc aacggtgttt
    15841 tatccaatgt attgaaagaa ttagaagctg gggcgtaagt cctagctttt ttctatgaat
    15901 catcataaac ctgttataaa tcgggaaaat tctatggtag aatgaaaaca caaaaaatat
    15961 ctgggagtgg ttagatggca gaattgatta acggaaaaga attagctgaa aaaatgcagg
    16021 cagagattgc tgaaaaaatc caaaaattga aagataatgg tatccatccc ggattagtcg
    16081 ttttattggt aggggaaaat ccagccagcc aaatctatgt aagaaataaa gaacgtgtag
    16141 caaaagaaat cggaatccat tcattggtag agcgttatcc tgagacgatt tctgaagacg
    16201 aactacttgc agaaattgaa aaatacaatc aagatcctag ttttcatggt atattggtcc
    16261 aattaccatt gccaaagcat atagatgaag aaaagatcct gttagctatc cgccctgaaa
    16321 aagacgtaga cggttttcat ccgatgaatt taggcagact ccttgcgggt aaaccggaca
    16381 agattccttg tactccatac ggcatcatga aaatgtttga agcttattct atcgatccgg
    16441 cgggtaaacg agcagtagtt atcggacgaa gcaatatcgt cggtaaaccg atggctcagc
    16501 tactcttgat ggcagatgca actgttactg ttgctcattc taaaaccgaa aatctggctg
    16561 aactggcaca agaagcagat attttagtcg tagcaatagg acgtggacat tttgtcacaa
    16621 aagaattcgt aaaaccaggt gctgtagtga tagatgtagg gatgaatcgt gatgctaacg
    16681 gtaagctgat cggtgatgtg aaatttgatg aagtggagcc tattgcaggc tggatcactc
    16741 ctgtgccaaa gggtgtaggg ccaatgacta tcacgatgct gatgtatcaa accgtaaaat
    16801 ctgccgaagc ttttggagca ggtgaataga tggcacaaga atatttaact gtcactacat
    16861 taacgaagta tttgaaaaga aaatttgatg ccgatcctta tttagaacgc gtatatttaa
    16921 caggcgaaat atcgaatttc cgtttacgtg ccaatgccca tcaatatttc agcttaaaag
    16981 atgaccatgc aaaaatatct gctattatgt tcaaaggagc attccaaaaa ttacgattcc
    17041 agccaaaaga aggaatgaaa gtccttgtcg ttgggcgtat ttcattatat gaagcgggag
    17101 gttcttatca aatctatgta gagcacatgg agcctgatgg aataggtgct ttatatcaag
    17161 aattggaaga acgaaaagaa aagctgatga aggaaggcct ttttcaaggg cctaaaaaaa
    17221 tgctgccacg tttcccaaag cgaatcgcgg tccttaccag tccaagcgga gcggttatcc
    17281 gcgatatcat cacgacggtg aaaagaagat atccaattgt ccaattggtt ttgttcccaa
    17341 cagttgtaca aggaaaccaa gcagcggaca atgttgtcag gaatatccag agagtagaag
    17401 agatgggcaa tttcgataca atgatcatcg gtcgtggggg tggttcgatt gaggatttat
    17461 ggccgtttaa tgaagagcgg gttgctcggg cgatttatca agctaatacg ccggtgattt
    17521 catctgtagg acatgaaaca gataccacga tcgctgattt agtagcagat gttcgggctg
    17581 ccactccaac agcagctgca gaattagctg ttcctgtttt gtcagaagag ttgatgcgca
    17641 ttcaagaacg gcaagctaga ttgcagcagg cgtttgtacg ccagatccag cgcaaacaag
    17701 aacggttcga gcgtgctcga cgttcctatg tatttcggca gcctgaaaga ttgtatgaag
    17761 gtcagaccat caaattagac cagttgaaac agcgattata tcaagctaca cagcaaattt
    17821 atcatgcgaa agaaaaacaa acgatcgcat taagccacca attggaacaa gtggctccgc
    17881 tttatcgagt taaagcagct cgccaagaaa ctgcttatct agaaaaacgc ttagaagaga
    17941 aaatccaaca atatatgaaa caacagcagc agcgttttgc tcaagctgtt caatctcttg
    18001 atttgttgag tccattgaaa atcatgggaa gaggctacag ctatacgacg ttaaatgatc
    18061 aagtagtaaa aagtgtcacc gagattcatc aaggggattt gctgacgatc cattatcaag
    18121 atggaatcgt taaaggacaa gtacaagaga tagacaaagg agaataaaaa tatggaaaat
    18181 ccaacttttg aagagtcact gcaagaatta gaaaaaattg tcatgcagtt agaacaagga
    18241 gacgttccat tagaatcagc tctagattcg tttaaacgag gaatggaatt aagcaaacat
    18301 tgccaagata cattagctaa agcagaagag accttgacga aaatgatgac agaatcaaat
    18361 gaagaagttg attttgacgg aaacgaggaa agctgagatg agattaactg acttttcagc
    18421 tattcatcta cctgctgtag aaaaagaaat cttgtctttt ttagatgagc atacaacgga
    18481 tagaggactc tatgatgcga tgagttattc agtgaaagca ggcgggaaaa gaatacgtcc
    18541 cttattactt ttaacggccg ttgcttcctt tgatgagcct attgatgttc ccgtctatca
    18601 agtagcagct gctttagaaa tggtgcatac gtattccttg atccacgatg atttgcctgc
    18661 gatggacaat gatgatcttc gtagaggaaa acctaccaat cataaagtat tcggcgaagc
    18721 attagctata ttggccggag atggtttgct tacaggagca ttccagctaa tcagtatggc
    18781 acacttaggt aattctccta agcttctgct gcttcagcaa ttagctgttt gtgcgggatc
    18841 acaaggtatg gttgctgggc aagctgcaga tatcgagggc gaatcgaaaa agctttcttt
    18901 agaagaatta gcatttattc atgaaagaaa gacagggcat ttgattcgtt atgccttgct
    18961 ggctggtggc atattagcga agcaacctga agaaatctta ttgctacttc aacgattagc
    19021 cgaacattta ggtttagctt ttcagataag agatgatcta ctcgatgtga ttggtacaac
    19081 gaaaacatta gggaaaacag ctggtaaaga tgaacgtatg gaaaaaaata cttacccgcg
    19141 tttattaggc ctagaaaaga cacgtgaggc gttggagatt gagctgttat ccgccaacaa
    19201 gatcatagac aaattagaag aggacgtttt ttcttttgat ggcgggctga tgcgccagat
    19261 gatcaagcaa tttgatgtgg agaaatgagg gaaagaatgg aaaaagaacg cgtagatgta
    19321 ttagcggtca aacaaggatt atttgaaaca agagaacaag ccaaacgttc agtgatggct
    19381 ggtttgattt acaacgaaaa aaacgagcgg tttgataaac cgggagaaaa aattccagtg
    19441 agtagtgaat taagagtcaa aggtaaaaaa cttccttatg tttctcgagg gggattaaaa
    19501 ttagaaaaag cactcaaagc atttgatcta tctgttgaag gaaaaacatt gttagatatt
    19561 ggtgcatcca ctggcggatt tactgatgca gctttgcaaa atggcgcaaa aatgagttat
    19621 gctttagacg ttggctacaa tcagctagct tggaaaataa gacaagatcc tagagtcgtc
    19681 gtaatggaaa gagtgaattt tcgttatgca aaacccgaag actttacctt aggaattcca
    19741 gaagttgctg ttatcgatgt ttcttttatt tcactaaaac tgatgctgcc tccattacat
    19801 gctattttaa aagaagaggg agaagtgatt gctttaatca aaccccaatt cgaagcaggc
    19861 agagaagctg tagggaaaaa cggaattgtt cgtgaccctc agacccatca aaaagtttta
    19921 gaagacattt tgtcatttgc ggcacaaggt ggatatgatg tgctggaatt aagttattct
    19981 ccgatcacgg ggggagaagg aaatatcgag ttcttggctc acttgaggaa agtacccgaa
    20041 agcggaacaa tcaattcagc tattaatatg gcagaagtgg tttcaaatgc acacgaacag
    20101 tttgaccata aataaattgt aactgtgaaa atcaagtgag aagtatgcgg attcttcttt
    20161 attataccaa ggaaataagc tatcattgat taagaagaat atgctaagga gcgaagacgt
    20221 aatgagaaaa aaagatcggc atcgtttgat tactcgattg ttgagtgagc aagatatccg
    20281 aaagcaggaa gaatttgttg aaatcctgaa aagcaaagga atatctgtta ctcaagctac
    20341 gatttccaga gatataaagg aactgaaact gatcaaggta ccggcttcag atggcggcta
    20401 ccgttatagc ataccagctg aaacaaacga agatgtaaat actaaattgg aaaaactatt
    20461 aaaagacgct ttcgtggcag ttgaccaaat ggaaaaattt gttattttga aaacattgcc
    20521 gggaaatgct tcggcagcag caaatttgat tgataaacgt tacaagaaag aattattttc
    20581 gattatcaat gatgacgata atgttttgat gattacacga actgaagacc aggcagtttt
    20641 attgaagaaa gacttcctgc attaccttta agttgaggag cgaatgtgat gctacaagaa
    20701 atcagtatta ctaactttgc gattattccg gaattgcggc tttcttttca cgaaggaatg
    20761 accgcgctga ccggagagac tggtgcgggt aaatccatta ttatcgatgc tttaggtctg
    20821 ttagcaggag gaagaggttc aagcgattat atacgtcaag gagcagaaaa atgtgtgctt
    20881 gaagggttgt ttgaactacc aaaacaagaa ggattctctg aactgatggt tgaattaggg
    20941 atcgaaacag atgaggacaa tctgattgtt cgacgggata tgtccttaac tggaaaaaat
    21001 gtttgtcgag tgaatggtca tattatcaca ttagctaatc tgagaagaat cggcagttat
    21061 ttagtagata tccaaggaca aaacgaacat caggaactgc tccagcctga atcacatctt
    21121 gctttattgg atcgttttgg agatgtagct ttccaacaga agaaaaagag ttatcagcaa
    21181 gaatatatct cttatcgtga gctggaaaaa agggtacgaa aagtgcagca aaatgaaaaa
    21241 tcctatgttc agagaataga tatgctccat ttccagcaag aagaaatagc tgctgctgat
    21301 ttgcaagtcg gagaagaaga gaaattaaaa gaagaaagag aaaaacttag caattatcaa
    21361 aaaattgctg atggattagc tgcaggttac ggagcactga cgaataatga acaaaacagt
    21421 gtagatggtg ttggcttggc cgtttcagaa ctgcaaggga tcgcccatct agatgtggag
    21481 tatgaagcaa tttatgaaaa tatccaaagt gcttactatt tgctgcaaga tgcaatcggc
    21541 gatatgagtc gtcagattga tttattggag ttagatgaga gtcgtttaga agaagttacg
    21601 caacgtttag aattaatccg acagttgaaa cgtaaatacg gcgaatccat tgagtcaata
    21661 ttggcttact atgacgaaat caccgaagag ctagcttctt ctgatttttc agaaggtcaa
    21721 ttggacaaaa tgaaagagga actagagcaa aaagagcttc tacttcagca acaagccgca
    21781 gatcttcatg aagcgcgtaa aaaaatcgca aaagaattgg aaaaatccat tctgcatgaa
    21841 ttgaaaagtc tgtatatgga aaacactgag tttgaggtac gtttcttaaa agaagaaaat
    21901 agacaattga atcatgatgg atttgaccaa atcgaatttt acattacgac caatccagga
    21961 gagccactga agcctttggt gaaagtcgct tctggtggcg aactgtctcg gatgttgctt
    22021 gctttgaaaa ctattttttc ttctgagcaa ggtgtgacaa gtattatttt tgatgaagtg
    22081 gatacaggtg tgagcggaag agttgcacag gccatcgcag ataaaatttc tcaaatatcg
    22141 aaatactctc aagttttgtg catcactcat ctgcctcaag ttgctgcagt cgccgactat
    22201 caatattata ttgtgaaagc agtgattgat ggacgtacgc aaacttctgt ttcagagttg
    22261 aaaacaaaag aaagagaaga agaaattgca cgtatgctgg ctggaagtga gattacaaaa
    22321 ctcacactcg aacatgcaaa agaactattg aaactcgcaa aaaggacctg agccacctcg
    22381 cagtggttca ggtccttttc agcagctgat agctatattt tatttttaca tgacactcat
    22441 tacagcagtc aatacaacag atccaatcgt taaaatcagc attgtccaaa tgacaaattt
    22501 tgttacttta gcaaaagtac ttgtttgttt tttttcacgc attgttttca acctcgtcat
    22561 taattagttg attcaagtgt acaatctttt ttccatttcc acaagagggc ttttttataa
    22621 aaacttccct ttttttaaat aataggtagt tacgatacaa ataaataatg ttaatagaac
    22681 tagccatata aaggcatcat ccctttcagc aataggtaac ttaatgttca ttccatagat
    22741 actgccgacg attgtcggta tagttaaaat aatcgttaaa gaagttaaaa ttttcataat
    22801 attattcaga ttattcgata cgattgctga aaaagtctga ttcatttgtt ctactagttt
    22861 taattgaatg cgagaagtag tcattgcctg ctctgtttcc accaatacat cacgtaaatg
    22921 atttttcaaa gcatgatttt cttggaaaag tttattttgc gctagttctt tcaatgcatc
    22981 taaattagca gaagtcgctt ctctgaaata aacgaggctt ttttggatat ccatgatttg
    23041 ataaagttgt ttgttttcag tggatacttg aagtttggct tccaaagtat cgcattgaga
    23101 ttgaagcgta gctaaataac tgttgtaaga gagtgtgacc tgccaaatga attgaaagaa
    23161 gatattgaga ttaggcgaaa tatcactttt tggaaaggtc tggctaaaga ctttctttaa
    23221 aaacagtggt tcatgattcg taattgtgat aagcttcttt ttgggcgtca aaataataga
    23281 gagcgggtat gtatccactt gaaaataacc gctaggactt gttttagcaa aaggatattg
    23341 taacaataat aacgctggct ttgtcaactt tgattgattg aattcttctg tacgggaatt
    23401 ttctgcatcg tccaatatgg aagtgatgta atctgacgga atttcaaatt gatgtgtcag
    23461 ctgatgaatc tcttcttgat taggacgttc tacacaatac cagatcgttt ggtcagtttc
    23521 ctttccggta ttacgcagtt tctcattatc taatgtaaaa tatttgatca tgattttcct
    23581 cctgttgcct gtttctttta ttataacgga tagggaattg gaatacatgg aatgtgtttg
    23641 taagatatag aatataactg cataaattta aataaaatga aacagttgtg taaagtatat
    23701 acagtatact tgaagcaacg ataaaaaagg agtttacgtt catgggcaca ttcacaccac
    23761 ttatcttgtt ttggtatatt tacccggtaa tcgttttgtt tggttgccaa tttcttgtaa
    23821 aattattttc gttaaatcgt cgttttaaaa taaaagcccc tgatttggcg attccttttt
    23881 tgtggggcgg tatgcatgca ttgtctagaa atacagggac aatttctatc ttaccgtttt
    23941 tctttatttc tgttttgcta atgggaattt gtattgctgt tttccaagca tattattatg
    24001 aagaaattat ttatccgcgt tatttcaaga tgacttggcg tttggttttt ctgttgacaa
    24061 tcgtcttgta cgctgtcttg atcattgtga acatactttc ttacctatga aaaaaataga
    24121 aaagctgtaa aaagtctttt aaaaggcttt ttgcagcttg atttttaaaa aaatgtttat
    24181 gaataaaaaa agttgttccc ggatttccca gaaaaggtgg tagaaagtgg gggattgtgg
    24241 tagactaatt ttagttagtg gaggaatggg gggctttcaa tgttgatggg tgaattccaa
    24301 cataatattg atgcaaaagg acgtttaatc gtgccctcaa aacttcgtga agaacttggc
    24361 gaaaagtttg ttctgacacg cggattggat ggctgtcttt tcggctatcc gatgagcgaa
    24421 tgggaaaatc tggaagctaa actgaacgaa atgccgttgg ctaaaaaaga cgcacgtact
    24481 tttgtgcgat tcttctattc ggctgccact gaatgcgaac ttgataaaca aggacgaatc
    24541 aatataccca gcacgttaag aaattatgca gctttgacaa aaggttgcgt agtcatcggt
    24601 gtatctaacc gaattgaaat ttgggatgag acacgatggc aagatttttc tgcagcagca
    24661 gaagagaact ttgacgaaat tgctgaaagt atgattgatt tcggactata agaataggaa
    24721 gaggctgact aatgactgaa acatttcaac actatacagt catgttgaaa gaaacggtag
    24781 atggattgaa catcaaaccg gatggcgttt atgtagactg tacactcggc ggtgccggac
    24841 atagtgaata tttattaagc caactaagtt ctaaagggca tctgtatgcg tttgaccaag
    24901 atcaaaaagc aatcgatcac gccaagctgc gtttagcttc ttatgtcgaa caaggtatgg
    24961 tcacatttat caaagcgaac ttcagagaat tagaagaacg cttgagtgag catgtagata
    25021 aggtagacgg cattttgtat gatctagggg tttcctctcc tcagctagat gaagcagaac
    25081 gtggatttag ttatcatcaa gatgcaccgt tggatatgcg catggatcag tcagctcctt
    25141 tatccgctta tcacgtaatt aatgaatatt cctaccatga attagtaaag attttcttcc
    25201 gctatggcga ggagaaattt tccaaacaaa ttgctcgtga aatcgaacga gtacgaaaaa
    25261 caaaaccaat ccaaacaact ggtgagcttg tcgaaatcat taaacaagtc atcccagctc
    25321 ccgcaagaag aaaaggcggg catcctgcaa aaagaatttt tcaagctgtg cggattgcag
    25381 taaatgacga actcggtgcc gaagaggcct ctttagaaca ggcaatccgg ttattgaaag
    25441 tcaatggaag aatcagcgta atcacattcc attcattgga agacagaatt gtcaaaagta
    25501 tgtttaaaga atatagtacg gtacaagatc taccaccggg acttcctgtc atacctgacg
    25561 aattccaacc agaactgaaa gtcattactc gtaaaccaat ccttccttca gaggaagagc
    25621 taacagaaaa caatcgatca cgcagtgcaa agttacgtat tgcagaaaaa ataagaatga
    25681 aagaggagta gaatgatggc agaattaaag aaaatgcaag attacgaata tgatttacct
    25741 gaattggtgg atcagccgga acaacaaatg cctgattctt cttcgaatca agaaatcatt
    25801 cttccacaat caccggcaaa acgattaaag cagatttctg ctttggaaaa aattgctgtg
    25861 gcaagcgtgg cgattgctat tgttgcttta tgtgttttaa cagttatgct gcgcacaaat
    25921 atcagcggag ctgaaaaaga aatcacgtca atacaaacag aaatcaatga taaaaagcaa
    25981 gaaaaaacga gtttagaaca agaaaaaaac gaactttctc gtactgaacg gattaaacaa
    26041 atcgcagaag aaaaaggatt gtctattaat gacgacaatt taaggaaagt gaagtaaatg
    26101 agtcttaaaa ataaaattcg gcggtttatg gaaaagaaaa atctaaatcc gatgaacaat
    26161 cgcaaaaaag taggtatcat tttatttgct acgagtattg gattgttctt tttatttgcc
    26221 gtccgtttct cgtatatcgt tattggaggt catgttgctg gtacctcatt agctgaaaaa
    26281 acaaaacagt tgtaccaagg aagtgaagta gtaaaagcaa aaagaggaac gatttatgat
    26341 cgtaacggtg tggcgctcgc agaagatgcc tcctcttatt caattaaagc gatactctca
    26401 aaaacatata cctctgggga taaaaaactg tatgttgaag aaaagaattt tgataaaata
    26461 gcagaaatcc tgcataagaa tctttctata gacaaaaaag atgcactgaa tattttagag
    26521 gacggcgcaa aaaaagagct gtatcaagtt gaatttggta gctatgggaa aaatatctcc
    26581 caagaaacca agcaaaacat cgaagcggac atgaaaaaag aaggtgtcgc tggtctttat
    26641 tttgtcgacc atcaggctcg gatgtatcca aatggcgtct tttcttctca ttttattgga
    26701 tacgcagttc cagacaaaga tgagaatggc ttagttggta aactaggttt ggagtctgct
    26761 tacaatgata tcttgagcgg taaagatggt aagattattt atcaaaaaga taattttcaa
    26821 aatcctctgc caggtacagt ggcagaagaa gaaaaagctg tcgacggaca agatatctac
    26881 acgactttag acagcagact acaaagttat ttagaaacac tgatggatca agtaaatgaa
    26941 gaatatcagc cagaagaatt gacagcagtc ctgatgaaag caaaaaccgg agaaatcttg
    27001 gcaatgggac aacgacctac tttcaatcct gaaacaatgg aaggattgac tggaaaagat
    27061 gctatctggc gtaatttctt ggtacaagat agttatgaac caggatcaac catgaaagtt
    27121 ttcactactg cagctgctat cgaagaagga gaattcaacg agaatgaaac cttccaatct
    27181 ggaaaaatcc aagtagaaga tgctacgatc aatgaccatg actttggtga aaaaggtgtg
    27241 ctgaccatgc gtcaagctct ttcatggtca agtaacgtcg ggatggtcat actggaacag
    27301 cgattaggcg gacgatggta taactattta caaaaactgg gatttggaca aagtactcat
    27361 tctggattag atgatgaagt aaacggtgct ttacctacat taaatattgt cgaccgagca
    27421 atgagtgctt atggacaagc ggtaggtgtg acgaatttcc aaatgatgaa agctttcact
    27481 tctattgcga ataacggtac aatgattcaa cctcgatata tcagcaaagt ggtggatccc
    27541 caaactggag aagagcgaac aactcaaaca gaagttctag ggcaaccttt ttcaaaagaa
    27601 acaacagaaa aagtccgtga atatatgaga gatgttgtcg aaagcgaaaa ctacggtagt
    27661 gcttatggtg tatatagtgt acctggatac aatgtatcgg caaaaaacgg aacagcccaa
    27721 atcgcttctg atacaggggg atatcaaaca ggagatacag cctacctgta ttcgattgtg
    27781 gagatggttc cttctgaaga tccagactat gtcttgtatc taacaatgaa acaccctaag
    27841 acatatgatc gaatggcatt ggctaaaatc gcaaatccat tgatgaaacg ggcaatggat
    27901 tttaaagaaa ctgaagaaga ttcagataca gaaacaaaaa cagaaaaagt atccgtagcc
    27961 gattatcgta atcttgaagc ggacgttgca gcagctgatg ctcaaaaaag tggactgcaa
    28021 cctgtagtga tcggtaatgg taaaaaagta caaaaacaat ctactgccaa cggtgatcag
    28081 ttgatctctg gagaaaaact gatcctatat actggtggtg ataagctgat gcctgacgta
    28141 acaggatggt caaaagcaga tatcatgaag ctggggaaaa tcctcggtat tgaagttagt
    28201 tttgatggag atggttattg tgtcaaacaa gaacttgctc catatgaaaa aatcactgaa
    28261 gacaaactta gttttacttt agaagaataa gaagagaaat aggagagaaa agagcatgga
    28321 atggacacaa gcattgattc ccattgtcag cagttgtgca atgacaattg ccgcaatgcc
    28381 gctatttatt ggttattttc aaatgaaaaa gcaaggtcag gcgattcgtg aggagggacc
    28441 aaagtggcac aatagcaaag ccggaactcc tacaatgggc ggcttagtgt ttctgatcgg
    28501 ctctattttg acaggaattt gggttggagc ttggcaaaaa cagctaactc ctactctttt
    28561 catcttgctt tttgtattag cgttatacgg agtaatcggc tttcttgatg attttataaa
    28621 aatattcaag aaaagaaaca tgggactgaa ctcgaagcag aaattgattg gacagattct
    28681 agggggaatc atcttttatc ttgtttaccg ttctgaaggt tatccaggtg tattgaattt
    28741 cttcggacta gatctacctt taggaatcat ttatggtatt ttcgctctct tctggcttgt
    28801 tggattttca aatgcagtaa atctgacaga tggcatcgat ggattagttg ctggcttagg
    28861 aagtatctct tttgcagctt acggtctaat tgcttggcat cagcatcagt atgatgtatt
    28921 aatcatttgc ttaagtgttt taggaggttt actgggtttc ttcgtctaca acagaaaacc
    28981 tgccaagatt tttatgggag atgtcggttc attagcgttg ggcggacttt tagcggcaat
    29041 ttctataatg ctaaaccaag aatggacatt gcttttgatt ggtttgatct atgttatgga
    29101 aacagcaagt gtgatgcttc aagttacttc ctttaagctt acaggaaaac gaatttttaa
    29161 aatgtctcca attcatcatc actttgaaat gtgcggatgg tcggagtgga agattgatac
    29221 agttttttgg ttgatcagca ttgtgacttc gttgatcaca ttatggatcg tttggtagga
    29281 gatgacggga aatgaaaaaa atatcaacat atgaaaataa gaaagtgctt gtcttaggac
    29341 tcgcaaaaag cggagtaagt gcggccaaat tattacatga attgggcgca cttgtcaccg
    29401 ttaatgatgg aaaacctttt gacgaaaatc cggaagcaca agagctgctt tctttaggga
    29461 taaaagtaat aacaggtagt catccaatcg aattgttgga tgaagaattt tcattgatgg
    29521 tcaaaaatcc agggattccc tacagtcatc ctttagtagc aaaagcccaa gaaatgggta
    29581 ttccggtaat tacggaagtt gagctagctt acgaagtagc ggaatgccca attattggga
    29641 ttacaggaac gaacggaaaa acaacgacaa caacgatgac aggactacta ctgaatgcag
    29701 gagcagatca aggaattgca cgtttagcgg gtaatattgg gtatccagca agtggtgtag
    29761 cgcaagaagc aaaatcagaa gataaaattg tgatggaatt gtctagcttc caactcatgg
    29821 gaatcacaga tttccgccct catatagcgg tgattaccaa tatctatgaa gcacatattg
    29881 attatcatgg tacaagaaaa gaatatgtca aagctaaatg gaaccttcaa aaaaacatga
    29941 cagaaaaaga ttatctgatc cttaactgga atcagtcaga actacaagaa ttagcgcaaa
    30001 gaacaaaagc acgcgtactg cctttctcga caaaagaagt gttggaagac ggtgtgtatg
    30061 ctgacgatta ctcaatatac tataaaaaag aaaaaataat ggaaatatct gaacttggcg
    30121 taccaggtaa gcataatgta gaaaatgcac tagctgctat ttctgtagca aaattgtatg
    30181 gtatatcaaa tgaagctata cgagaaacgt tgcatttttt ccatggcgta ccacatcgta
    30241 cgcaatatgt aggagaaatc caaggcagaa aattttataa tgattcaaaa gcaaccaata
    30301 ttttagcgac taaaatggct ctaagtggat ttgaaacgtc taaggtcgtg ttattggctg
    30361 gcggattaga cagaggaaat acatttgatg aattaattcc ttcattaaaa ggaatcaaag
    30421 caatggtcgt atttggccaa acaaaagaaa agttgatgga tgctggaaaa aaagcaggca
    30481 tcgagacaat agtaacagcc gattctgtag aacaagcagt tcctttagca ttagaaaaca
    30541 gtacagatgg tgatgtagtt ttattatcac cagcgaatgc cagctgggat caatacccaa
    30601 attttgaaac tagaggaaac agattcatgg aagctgtgaa ccgattaaaa tagaaaaagt
    30661 agaagcgagc gataaaaaat gaaaattttg gttacaggcg gaggaacagg gggacatatt
    30721 tatccagctc ttgcctttgt caattatgta aagactcaag aaccgaatgc tgaattcatg
    30781 tatgtgggag caaaacgagg tctagaaaac aaaatcgtcc ctgatacagg aatgcctttc
    30841 cataccttgg agattcaagg atttaaacga aaaatttcca tgcataacat caagacggtc
    30901 cgattgtttc ttaagagtat ccgtcaagca aaaaaaatcc tcagtgattt tcaaccagat
    30961 atcgttatcg gtactggagg atatgtttcc ggagcggtag tgtacgctgc agccaaaatg
    31021 ggtattccaa cagttatcca tgaacaaaac agtgtaccag gtgttaccaa taaattttta
    31081 agccgatatg tggatcggat cgctctttca tttaaagatg cggcttcctt ttttcctgaa
    31141 aacaaagcag ttcttgtagg taatcctcga gcacaagaag tagcagatac taaaaaatca
    31201 gatattttga aaacatttga tttggatcca gaaaagaaaa cagtcttgat ttttggcggt
    31261 agtcaaggag cacttaaaat caatcaagca gtatcagttt ttttaagtga atatattcct
    31321 tcttcttacc aagttttgta tgcttcagga gatagatatt atgaagagat cctaaataaa
    31381 ataaatcctg aaatacaaaa tattagtgtc cagccttata tcaaaaatat ggcagaggtc
    31441 atggcttcca gtgatctgct ggtggggcgt gctggtgcaa cttcgatcgc agaattcaca
    31501 gctctggggt taccggcagt gttggttccc agcccatatg ttacgaatga tcatcagact
    31561 aaaaatgcaa tgagtcttgt tcacgcagga gcagcaaaaa tgattgcaga taatgaacta
    31621 acaggtgaat cactaagtca gacagtcaac gaaatcatgg gtgatgaaga attgcaaaag
    31681 caaatgtgcc gagcttctaa agaacaagga atccctgatg cttcaaaacg tctttatgac
    31741 ttagtaaaac aaataatcaa aaaataaagg cggtggtctt agtgagtaag aaaaaagaac
    31801 caaaaaaatt gtctcatccg tcttctgatg aaaacaattt gactccttgg caaaaggcta
    31861 accgaaaata tctggcagaa caagagatga aaaaccaaga gccaaaaaaa gaagacataa
    31921 cattagatac acccctttca gattctgaag aagaaaaaac ggttcaagag gaacagcaag
    31981 aagaaagcca gtccgagaaa aaaatcgaat tttttgaaga gatcaagcaa aagaaaaacg
    32041 gtggaccata taacggttct tttttgaacc ggctgcccaa tttaaaaagt caacggcaca
    32101 aagtactatt tcgtcggtta gctttgatca tcactgtatt gggtataccg ctgatttttt
    32161 tgatctatta tgtttctcct tatagcaaat tacaagcagt tgcagtatca ggcaataaga
    32221 cagtcaacag ccaagaaatc atctctgata caaaattgtc attaggagaa aatgtttggg
    32281 gacagtattt tcatcgatct acttatatcg atcgccttaa aaaagcacag ccaagaatcg
    32341 aaacagcaaa tatacatttc aaagggatga atgaattcga tttggatgta acagaatata
    32401 aagagattgc tttgatcgca aaaaacaatc aatactaccc agtgattgaa aatggaacag
    32461 tattagatga aaaagtagca aatcctacaa aaaatctccc tatcttggaa gagttcaaag
    32521 actctgcaaa aatcaaagaa ttaacaaagc aatacaatca gttatcatca gaattacaaa
    32581 aagcaatttc tgaaatcaag tacacgccaa gagcaagcaa taaaaatctg atccaattga
    32641 atatgaatga cggcaatcaa gttattgtaa atatcaataa tcttgcgaac caaatgaaat
    32701 attattcgca agtagccaaa gatatggatg aaaaaggtgt gatcgacatg gaagttggta
    32761 tcttctcgca tccttacggt agcactcaag aaacggaaga atcgggccaa tcggaagaaa
    32821 caactgaaag tgaagaaaat gcggatcaaa caaacgaatc aagtgaagag gcagaaacgc
    32881 aaaaagcaca aacacctgga tcgtctagca caaacggatc atctgattca aatgattcaa
    32941 gtgaaacaag tcaaatagaa gataatccaa gtacaactcc gtcaagttta aatgaaaatt
    33001 gataaaacaa ctttaaaaag taagaatgta ctttctaaaa accagtaatt tatggtagaa
    33061 ttggaagcag tgaatatatc tattaataag tatattttaa aacggacgct agttgttcgc
    33121 ttgtatatgg aatagtaagt aggagggaat cccattcatg gcaaaaacag gaatgtatgt
    33181 aggccttgat attggaacga catctgttaa agttgtcgtg gctgaatata tcgacagcca
    33241 aatgaatatt attggagtag gaaacgcaaa atcagaaggg attaaccgag gcattatcgt
    33301 tgatatcgat aaaacggtcc aagcgataca acgagcagta cgacaagcag aagaaaaagc
    33361 aggtatccag atcaaaggcg taagtgtcgg tttgccagca aacatgcttg aagtagaaaa
    33421 ttgccaggga atgattgcag ttaacggaga ttcaaaggaa atcaccgatg aagatgtcag
    33481 aaatgtggct tcagctgccc ttgttcgttc aatacctcct gaacgtcaaa tcgtgtcaat
    33541 attgccacaa gattttactg tcgatgggtt cgagggaatc aaagatcctc gtggaatgat
    33601 cggcgtaaga ttagaaatgt acggtctgtt gtttacagga cctaaaacga tcatccataa
    33661 catacgtaaa tgcgtggaaa atgccggatt aatcgtcaac gaaatggtga ttactccttt
    33721 agcgctgact gaatcaattc tttctgacgg agaaaaagat tttggtacga tcgtcattga
    33781 tatgggtggc ggccaaacaa caacggctgt tatgcatgat aagcaattga agtttaccaa
    33841 tcttgaccaa gaaggtggcg aatttgtcac taaagatatc tctattgtat taaatacgtc
    33901 atttaacaat gcagaagcgt tgaaaataaa ctacggggat gcatacccag aacggacatc
    33961 accagatgaa gaatttccag tagatgtgat tggtcagtca gaaccagtga aagtcgatga
    34021 acgctacttg tcagaaatta tttcagctcg aatggaacag attttcaaca aagcaaaaga
    34081 tgcattggac caaatcgaag cattagagtt gccaggtggc gtcgtattga caggcggagc
    34141 tgctagtctt cctggtgttg tggacttggc acaagaaatc ttcggcgtaa atgtaaaatt
    34201 gtatgttcca aatcacatgg ggctgagaaa cccggtattt acaaatgtaa taagtatcgt
    34261 ggattattca gcaaacttga gtgaagtgta tcaattggca aaaattgctg ttacgggtga
    34321 aacctcagca gctcgtcaaa tggtagttga acaagaaaca acaaacacat atgagagcta
    34381 tgaagcacct gaagaaacaa tctacgatga gccggaacca aaagagtccg gagaaaatgt
    34441 aaaaaataaa atcaagggtt tctttacaaa tatttttgat taattcatcc tgggaggaat
    34501 aagatatgga attctcaatt gataataata tcaatgacgg cgcagtaatc aaagtcatcg
    34561 gagtcggtgg tggcggtgga aatgctgtaa accgaatgat tgaagaaaac gttaaaggtg
    34621 ttgaattcat tacagccaac acagacgtgc aagcattaaa aaattcaaaa gctgaaacag
    34681 ttatccaatt aggccccaaa tatactcgtg gtttaggtgc tggttctcag ccagaagttg
    34741 gacaaaaagc ggcagaagaa agtgaacaat ccttacgcga agctttagac ggtgcagata
    34801 tgattttcat cactgcagga atgggtggag gaaccggtac aggtgctgca ccaatcgttg
    34861 caggtatcgc gaaagaattg ggcgcattga ctgttggtgt cgtgacacgt ccattcactt
    34921 ttgaaggacc gaaacgtggc cgttttgctg cagaaggtat cgctcgtttg aaagaaaatg
    34981 tggatacatt attaattatc tcaaataacc gtttattaga agttgtagat aaaaagacac
    35041 cgatgcttga agctttccgt gaagcagata atgttttacg acaaggtgta caaggtatct
    35101 ctgatttgat cacagcgcca ggttacgtta accttgactt tgctgacgtg aaaacagtga
    35161 tggaaaacca aggtacagca ttgatgggga tcggtgtggc tagtggagaa gatcgcgtag
    35221 tagaagcaac gaaaaaagca atctcctctc cattgcttga aacatctatt gatggagctg
    35281 aacaagtact tcttaacatc acaggcggtt tggatatgac attgttcgaa gcacaagatg
    35341 cttcagatat cgtagcaaat gcagcaactg gcgatgtaaa tatcattcta ggtacatcta
    35401 tcaatgaaga aatgggcgat gaaatccgtg tgacggtcat cgcaacaggt atcgatgaat
    35461 caaaaaagga acgcaagtct agccgaccag ctagacaagc acaaatgcaa tcacctgctc
    35521 aaaagactgt attagatatg gatcaagcta aaccaacttc ttcagaagaa gaaaacagtt
    35581 ttggcgattg ggatatcaga cgtgaacaaa atgttcgtcc tagagttgat gattccaatt
    35641 ttgacaatat cgaaaaaaaa gaatttgata cattcaaccg cgaagaaaca aaatctcatg
    35701 gcgatgatga attaaatacg ccaccgtttt tccgtcgtaa aaaataaagg agaggaagag
    35761 cacatgattg ctgacaactt gcagaaaatc aagcaagaaa ttcaggattc gtgtgctctt
    35821 gttcatcgtg atccgaaaga agttacatta gtagctgtaa caaaatctgt tgaatccgct
    35881 caagctgaag aattggttag agctggcgtt atgaattgtg cagaaaaccg agtagataaa
    35941 ttattagaaa agaaacaaga tttatttgat tttcctgaaa taaagtggca tttaattggt
    36001 aatttacagc gcagaaaggt aaaattgatt gtaaatgaaa ttgattactt tcatgcgttg
    36061 gacagtttaa gattagcaga agaaatccag aagcggttag agaaagaatt atcctgtttt
    36121 attgaagtaa atgtttctgg tgaggaatca aaacatggaa tttcaccaga tgagctttta
    36181 ccgtttgtgg aatctttagc tgcctgcaca aaaatcaagg ttgttgggtt gatgactatg
    36241 gcgcctaaag aagcttctga tgagcaaatc cataaagtgt ttggtacgct gaaagaattg
    36301 cgtgatcttg ttcaagcaaa aggttatgcc catgcaccat gtagtgaatt aagtatgggg
    36361 atgagtcaag actttcaaat tgcgattgaa gaaggtgcaa catttatccg tgtaggaaca
    36421 gcgctattca aagacgaata aaggaggaac aaaaatgccg cttttaaaca gaggagcaat
    36481 tgctaatttt ttcggattag ctgatgagga agagtacgaa taccaagatt atcctgaaac
    36541 aacaaaaaag gctgtacagc caactaaaaa aaatacaaca cagaatcaat caactcattc
    36601 tgtatctgct gcgaatactc atgaaaaaca aaaaacggcg acttcacgtc ctgtaacgca
    36661 tgaaagtatt cagccggctg aaaagaaaaa agtaattgaa ctacatcaat cacctacaaa
    36721 aactgtagcg aaagaaagaa cagcttcaag aacctctaca caacctaaag caaatcttgc
    36781 tggaaaaatc actgtaatgg aaccaagagc ttattctgaa gctatggcga ttgctaaacg
    36841 aatcattgct ggagaagcaa ccttagtgaa ttttcattta gtagaagaaa aacaagcgag
    36901 aagaatcgtt gattttctaa caggaacagt ttatgcacta gatggagata tcaaacgtgt
    36961 tggagatgag atttttcttt gtacaccgac tgggatggag attgacagtt caaccgctca
    37021 atcttttgca gatgcgaatt tgtttgatct gtaagaggag gaactacgat ttatactatt
    37081 gcactattac ttcatttact tcaacgggca gcctatttct atagcattat cttgatggta
    37141 tatgcattgc tctcttggtt tccaggcggg tatcaatcag ggctaggacg attattagct
    37201 aagatctgcg aaccgtatat cagtttgttc gatcgtctgc ctttgaggat tggacccttg
    37261 gatttcacaa ttttaggggc aatcctgatc ttgaacttag ccgtacaagg tttagcacag
    37321 atcgttatat ggataactgt ccatttagtt tattgattga agcaacaaaa tctttattct
    37381 cagaatagat aggaggggaa aattttggat gcaaatgtat accagcactt tcgggcagat
    37441 gaacgtacat ttatcgattc ggtaggcgat tggattgaac aggttcaagg acaatatgca
    37501 ccctatctga ccgaatttct tgacccaaga caatcctata ttttagaaac actgattcga
    37561 caaagcagtg atttgcgttt catgttttat ggcggatatg aacaggcaga aagaaaaaga
    37621 tgtttgattt tccccgatta ttacgaacct gtagaggaag attttgaaac agcattgtat
    37681 gaaatccatt atcctagcaa atttgccagc ctaagtcatg ggaaaatcct tggaacttta
    37741 gtgggaacgg ggatcaaacg ctcttacttt ggcgatatca tctcggacgg tgatcgctgg
    37801 caagtattta ttgcaaatga ggtggatcat tttgttgctg cacaagtaac aaagattgga
    37861 aaggttgctg tacgcttgga accaatcaaa tacacagaaa tgattatgcc gaaagatagc
    37921 tggacacaag aaagaggaac agttagttcc atgcgactag acagtgtgat ctctgagatt
    37981 tataatattt ccagacagcg gtccaagcag ctgatcgaag ctggtaaagt aaaagtgaac
    38041 tggacagaaa atacaagacc tgattttgta cttgaactat tagatattat ttcagtcaga
    38101 ggttatggca gagtccagat ccaagaactt gaggggaaga cgaaaaaaga aaagtacagg
    38161 gtactgttcg gtgtattacg taaataaaat aaaaaggtgg tttactcatg gctttaactc
    38221 cattagatat ccaaaataag acattcccga caaaaatgcg gggatataat caagacgaag
    38281 tagatgattt cttagattta gttgttcgtg actatgagga attgactcaa agaaatcgag
    38341 aattagaaaa agcagtcaaa cattctgaag aaaaacttga gtacttcaat gaattaaaag
    38401 atgctttgaa ccaatcaatc atcgtagctc aagatacggc tgataaagta aaaacaagtg
    38461 caagcaagga atccgaagtg atcgttactt ctgcacaaaa caaagcagat gagttagttg
    38521 cgaacgctga aaaacgtgca catcaattaa caactgatgc agaagaaaaa gcaagaaaaa
    38581 tcttgacaga tgcaacagaa aaagcaagac agcttgcgac agaaactgaa gatcttaaga
    38641 aaaaaacacg agtattccat caacgtatca gcttgatgct tgagtctcaa ttagaacaag
    38701 tgaaaagtcc ggaatgggat gaaattttac aaccattctc cagctacgtg actgacagcc
    38761 atgaagtgat caaagaagtt ttagcaaaac agcttgacaa tgaaaatgat actgatgtaa
    38821 actcagacac aggagtcata gaagcaccta tgacggcatt tgatacacag gctattgata
    38881 tctcgatcgt gcctggtgaa aatgaaaacg agtagaacag aaaagcactt tgttattctt
    38941 aaagcgagcc gatgagagtg gaagttcggt aagacactgc atggtgaaag atcctctact
    39001 tgagacctgc ttttccgaaa ttagagtaag aagcagcggc tgacctcgtt attggtcaca
    39061 gagagtttgc actagataga tgcaactaaa tttgggtggt accacggcat gttcgtccct
    39121 tgcggctgaa catgcttttt tgtgtccgga aaacaaataa aggagtgtat ggttgaatga
    39181 aaatgaaaga aaccttgcaa ttaggaaaaa caaaattccc tatgcgtggt aatttgccaa
    39241 accgagaagg cgagtggcaa aaagaatggg aagaaaatcg aatctaccaa aaaagacaag
    39301 agttgaacga aggaaaacct agctttgttc ttcatgatgg acctccatat gccaatggta
    39361 atatccattt aggacattca ttgaataaaa tcagtaaaga cattatcatt cgttcaaaat
    39421 caatgtctgg ctatcgtgca ccatatgttc caggatggga tacacatgga ttgccgattg
    39481 aacaagtatt ggctaataaa ggcgtgaaac gtaaagaaat gtccatggca gaatacttgg
    39541 aaaaatgtcg tgaatatgca ctttcccaag tagataagca acgagcagat ttcaaacgcc
    39601 taggagttgc aggagattgg gaacatcctt atgttacatt agatccttct tatgaagctg
    39661 cacaagtacg cgtatttggt aaaatggctg aaaaaggcta tatttacaaa ggattgaaac
    39721 caatttactg gtctccgtca agtgagtctt ctttagcgga agctgaaatt gaatataaag
    39781 atgtaaaatc tccatctatt tttgtagctt tcaatgtcaa agacggcaaa gatattcttg
    39841 atgaagatac agcttttgtc atttggacga caacaccatg gacattgcca gcaaaccaag
    39901 gtattgctgt aaatccaaaa tttacgtatg tattggtgga agccgatggc cgtaagtttg
    39961 tcgtagcgaa agatttgcta gaaacagtcc aatcagaaat cggttgggaa aatgtaacag
    40021 ttctaaaaga atttgctgga caagaaatgg aatatatgac tgcaagtcat ccattctatg
    40081 aacgtgaatc acttgtgatt ttaggcgatc atgttacttt agacgctggt actggattag
    40141 tccatactgc tccaggacat ggggaagatg actatatcgc tggtagcaaa tataaactat
    40201 ctgtagtgtc acctgtagac tcaaaaggat tgtttacaga agaagcacct ggatttgaag
    40261 gtatctttta tgacaaagca aatcctatga tcactgatct attaaaagaa aaaggagctt
    40321 tgctgaagct agatttcttt actcatagtt atcctcacga ttggcggacg aaaaagcctg
    40381 tgatctatcg ggcaactcca caatggtttg catctatcga taaattccgt cagaatattt
    40441 tggatgaagt agaaaaagta gagtgggtta tcccatgggg taaaacacgt ttgtataata
    40501 tgatccgtga ccgcggcgat tgggtaattt cccgtcagcg tgcatgggga gtaccacttc
    40561 cgattttcta tgctgaaaat ggagaagcaa tcatcacacc agaaacaatc gaacatgtgg
    40621 caaaattatt tgctgaacat ggatcaatta tctggtttga aaaagaagcg aaagaacttc
    40681 ttcctgaagg atttacccac ccaggttctc ctaatggcga atttacaaaa gaaaaagata
    40741 tcatggatgt ctggtttgat tctggttctt ctcatgaagc tgttttacgt cagcgaccag
    40801 aattatcatt cccagcagat atgtatttag aaggatcaga ccaatatcgt ggctggttca
    40861 actcaagtat cacgactagt gtggctatca atggtgtagc accatacaaa tcagttattt
    40921 ctcaaggatt tgctttagat ggtgaaggcc gtaagatgag taaatcttta ggaaacatca
    40981 ttgcaccaga aaaagtgatc aaacaatttg gtgcagatat ccttcgctta tgggtatcta
    41041 gtgtagatta tgaagcagat gttcgtgttt ctatggatat cttaagtcaa gtggcagaag
    41101 tatatcgtaa aatcagaaat acgatgcgtt tcttattagc gaatacagaa gatttcgatc
    41161 ctaaaaaaga tactgtttcg tacaatgatt tacgttctgt agacaaatat atgaccgttc
    41221 gattgaacca agtaatcaaa gaaatccgag aagaagggta cgacaaatat aacttcatgc
    41281 atatttaccg tacagttatg aacttcttga cggttgatct atcgtcattc tacctggatt
    41341 ttgctaaaga tgtagtttat attgaagcag aagatgatta tcaacgtcgt tgcatgcaaa
    41401 ctgttttcta tcaaacagct gtagctttga caaaattatt gactcctatc attccgcata
    41461 cagcagaaga aatctggtca ttcctgaaag aagaagagga gtacgttcag ttaagcgagt
    41521 ttcctggtta tgaagagttt gcaaaccaag aagaattgat ggatacctgg actgctttca
    41581 tggacttccg tgataatgtc ttaaaagcat tggaagaagc aagaaacagc aaattgattg
    41641 gtaaatcttt ggaagcaaaa ctaactgttt atccaaaaca acaagttcgt gaaatgttaa
    41701 aagctttgga tgcagatatt gcacaattat tgatcgtttc accagactac tttgaagtta
    41761 tggcagcaga tgaacaagtg ccagataacg ctatggtatt tgatgatgtt gctattacag
    41821 tggaaaaagc agacggggaa acttgcgatc gctgtcgcca agttcgtaaa gatgtaggag
    41881 tagatgaaaa acttcctcat ctatgcggtc gttgtgcaaa aatcgttgaa gacttctatc
    41941 ctgaagctgt agctgaagga ttcgaagaaa agtaaaaggg aaaagggggt gtgacatgtt
    42001 ctttgtcaca cccccttttg ttatcaatcg tttaattttc cacgttcacg ataccagtta
    42061 tctggttgcc agatccattc gaatccgtct ttttcaagta gttcgaaagc tgctttaggt
    42121 cccattgatc cagctttgta gttgggaaaa tccggttgtg tttggtccca agcttttcga
    42181 atgacatcca cgatatgcca agattgagcg acttcttccc aatgagagaa gttcgttcca
    42241 tcaccattca acgcatctaa tagtaatttt tcgtaagctt ctgggctatt ttcaaccatt
    42301 tctgcactat ttctaaattc caatttgatt ggttccgttt caaatccttg ccctacttct
    42361 ttcccattca aggataaaga aaatccttca gttggctgaa tatagatcgt tagaacatta
    42421 ggtggaagtg tcgtatcgtc acatggttca tcgacagatg ttttgaatac attaacagga
    42481 acttgtttga atacgatgtt gatccgagtc cctttttcag tcaaacgttt gcctgtacgt
    42541 acatagaaag gaacacctga ccaacggaag ttatcgatag tgaatttgcc tgcgacgaat
    42601 gtttcagtct ctgattgagc atccactttg tcttcttcgc gatagccctt gaattgtaca
    42661 ccatccaatt caccttgagc atattgcccg cgaacgaagt tttctaacac ttcttcttcg
    42721 ttataaatgc ggatagcttt caatgctttg atcttttctg tacggatttc tttttcggaa
    42781 aatgctactg gcggttccat tgcaagtaat gctacaacct gtaaaatatg attttgcacc
    42841 atgtctttaa gtgcgccact atgatcgtag tagcccccgc gatcttctac accgaggttt
    42901 tcgccaaaag taatttggat attgtcgata tagcgattgt tccattgagc ctcaaaaata
    42961 ttattggcaa aacggatagc tgagatgttt tggatcattt cttttcctaa ataatgatca
    43021 atcctgaaaa tatcttgttc tgggaatact tctctgattt gctggttcaa ttcaaaagca
    43081 gactggtaat cagaaccgaa cggtttttct atgatcaaac gattgaagcc attttttgtc
    43141 ataatatttt gtgatttcaa atgagaaacg atcgttccaa aaaattgagg tgccatagcc
    43201 aaataataga cacggttacc gtttaattga tagtgtccat ctaatcggtc agacaattct
    43261 tttaatgtct gataatgcgt tgaatcattt acatcatggg attgatagta aaaatggctt
    43321 gcaaaggtag ttgcttcttc atctgaagga ttcaatgcac ggattgtttc tttgacaact
    43381 tcacggtagt gctcatcgct ccagggacgg cgagcggttc cgattacagc aaaatgctcg
    43441 cctaaatttc cttttctgta aaggcggaac aaagacgggt acaatttacg ctgggctagg
    43501 tcacctgtac cgccgaatat cgtaaacaat acattttttt cattagacat gtggatacac
    43561 tcctttatat tttatcgaca aaaatcgtgc tggccgcctt aaagctgacg gggattttct
    43621 ttctatcatt ttggatagtg atcggtcctt cataagctgc gatgtcaacg atctcatatt
    43681 cttcgttgat attcaggtca atagtgacta agtaatccaa caattctttt tcatcaagca
    43741 ctcgtgcgat acgcactttt gtaccgacag gataggactc caatgtttgt cggcgttctt
    43801 catggacaac ttcctctttt ttaggaatcg atcctccatg aggacaatgt tcaggatgat
    43861 tcaaatagtt ttctaaatga tctgcaagat gttccgaagt cacatgttcc aatacttcag
    43921 catcatcatg gacttcattc cagctatagt ctaaatgttc aactaaaaaa acttcccata
    43981 agcgatgttt gcgaatcaga ctgctagctt tttgcagtcc gcttgcagtc aattgtacac
    44041 cttgataagg ggaatgttct acaagctctt ctttcacaag tttagaaatc atttcactga
    44101 cagatgcagc tgagacatct agactagata cgatttgttt attgttgact ttcgttgtat
    44161 ctccacctag ttctaaaatg attttcaaat aatcttctcg atttggtgtc atattcgatc
    44221 atcctttcat ggtaaaaaac catcattagt aagtgtaaca aaaaaaacgt taattgacta
    44281 tttttctaaa taaaaaacaa aggattttta gacgttttta caacaaaaaa cgaaagatga
    44341 tagagaagac tcattctttc gtttataaag gatatatata tacattggga gggatctatt
    44401 tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt tgttcgggat
    44461 aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat tcatctattt
    44521 ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca gatgtaatct
    44581 gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac tttgagagtc
    44641 cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag agaacggtaa
    44701 tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt tatgagaaaa
    44761 taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa gctaaaattt
    44821 ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata ggatacatag
    44881 taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt ttttattgac
    44941 cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat tattgcgcat
    45001 gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt ggctattgta
    45061 tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa aaagaagggt
    45121 gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg tattttagac
    45181 ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg tgaagtaact
    45241 tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa agcatttgtt
    45301 gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga tgcgcccaca
    45361 ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg ttatgccgct
    45421 tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca tgaagaaatg
    45481 caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca aatcctcgcc
    45541 tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc atttgatgta
    45601 gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat caacttaggt
    45661 attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag cacaaaaaaa
    45721 tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt tggttctcat
    45781 tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat tactcatatg
    45841 gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa gcaagcaatt
    45901 ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa agaaatggat
    45961 gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc aagaaatatc
    46021 catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga gataatcggt
    46081 gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga tggagaagaa
    46141 ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg tgtttacctt
    46201 gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt cccaagaaag
    46261 atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa ttttgatgta
    46321 tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta ttttgactca
    46381 ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca tgatcgcgcg
    46441 cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc agatgaagca
    46501 gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt gttgtatgaa
    46561 gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag aaaaaatggc
    46621 ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt gtacaagtat
    46681 ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct tctcttattc
    46741 aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt tctcctacag
    46801 gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa ccaggtgaag
    46861 caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta gcagatgttg
    46921 cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc ggagccaacg
    46981 tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt acaccaggaa
    47041 gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa acaatggtat
    47101 ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat caaattcttc
    47161 atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat cgttcccttg
    47221 aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca gcagaggacg
    47281 attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag aaagatgact
    47341 acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt aatcaactga
    47401 ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta ggaagtctgg
    47461 cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa aagaaaatgg
    47521 tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca ggtcttcctt
    47581 atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt tcgggaagaa
    47641 ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat aatctaagag
    47701 atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt ttatacggag
    47761 gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta aataaaaagt
    47821 atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa aaaataagaa
    47881 aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc aaataagcgc
    47941 ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga ggggttatgt
    48001 gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag gattgacagc
    48061 aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat atttaagtga
    48121 actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt actctttgcc
    48181 aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag tgacttttga
    48241 aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa aagctgcgat
    48301 tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta cagggaagtc
    48361 gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt ctgcagatgc
    48421 gccatttgtt tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc tctttggaca
    48481 catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc ccggtctaat
    48541 agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac cgccggaagg
    48601 gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg gagaaagtgc
    48661 acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt cagaaagttt
    48721 cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc ttgcttcacg
    48781 ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag cgaatcgttt
    48841 gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt atgatgcgga
    48901 aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat cttttttaca
    48961 ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt cccttcaagt
    49021 acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat tcatggacag
    49081 caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt cacaagatcc
    49141 agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc tttccgaaac
    49201 cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg cttatttcca
    49261 gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc cagatgaaat
    49321 ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc gtatttataa
    49381 tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc gtgtgagaga
    49441 aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta agagggaatt
    49501 ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg agattccttt
    49561 tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa gtgagcctgt
    49621 taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg cttcaagcat
    49681 gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta atatgccact
    49741 ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa actatcaaga
    49801 gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca ccttcagtga
    49861 tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa gtaccatgat
    49921 tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata tttatcaaaa
    49981 tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa agtctgaaac
    50041 tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag tagctgccaa
    50101 attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga tccagatgca
    50161 gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag aatatgagat
    50221 caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt tttcagccta
    50281 tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg atgagatccc
    50341 gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt ctattgatga
    50401 actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt cgattatcgt
    50461 ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag acggattgat
    50521 caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa tttatcgttt
    50581 ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc aggtaaaaat
    50641 tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa taaaaaaatg
    50701 aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata aaaaagagct
    50761 aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct tgcgttatat
    50821 aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc atgttagttt
    50881 gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca gcagaagaaa
    50941 aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat aatttagaat
    51001 ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa gctcaatttg
    51061 aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa gactatggca
    51121 tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa atcaagtatg
    51181 cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca tggggttaat
    51241 catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg ctaaaatcgg
    51301 tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta tacaagcgca
    51361 tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc aagtgacatt
    51421 attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg atttagcaaa
    51481 agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc ttttttgaac
    51541 ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac tctctttata
    51601 taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat ggagaaatac
    51661 atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt gcgtgatgct
    51721 tttatcggca cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat caatgcaatt
    51781 attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc agcagacaaa
    51841 ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg gaacggtaca
    51901 ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc ccgagcttat
    51961 ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca aggtatttcc
    52021 tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt cgcagatgca
    52081 atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt tggattatgg
    52141 ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat gatcatggga
    52201 gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat caaaatgcct
    52261 gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc gacgatcgct
    52321 ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg gcaactgatc
    52381 attgatttgg tgcaaaaata tattgcagag ccattcctag gattatctca agggttggga
    52441 gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat tcatggacca
    52501 aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat caatattgat
    52561 attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga tgcaatcgaa
    52621 gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg gtttggcgga
    52681 tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg tgcagattat
    52741 ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga accagtgatg
    52801 tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat tgctccagta
    52861 gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc ggtatcgcag
    52921 caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg agcggattgg
    52981 cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg ggcaccattt
    53041 gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga tgaacgtgag
    53101 gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa ctgaaatgat
    53161 tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag gatacaatga
    53221 agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct ttgaaacaag
    53281 tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag cacagccttg
    53341 gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa aagataggat
    53401 ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga tcgtgttgta
    53461 tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag gtctttttct
    53521 aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag caaaggagaa
    53581 aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt ttacgataaa
    53641 gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt aactagaaaa
    53701 cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc tcgcataaaa
    53761 atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac gaagaatgaa
    53821 ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt tcattacaca
    53881 gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt aggaacaatc
    53941 ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga agcaacgtat
    54001 taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct agaaggaatt
    54061 tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta ggaaggggca
    54121 gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa atgtttgatt
    54181 tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt tttcgtacac
    54241 ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta acaagtcaag
    54301 tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg gccggcattg
    54361 gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag atcatggaaa
    54421 agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg ccattctcct
    54481 atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt tatcaactaa
    54541 acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt atggattttg
    54601 aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac caacgagctg
    54661 gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc aattaccagt
    54721 ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat cagcttgcaa
    54781 cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt tgcagccttg
    54841 ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca tgcggctacg
    54901 gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg atcacacctt
    54961 atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca tttaaaaaga
    55021 gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg aatgaaggaa
    55081 tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa caaacaaaac
    55141 tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg tatcgtaaag
    55201 tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa gaaacgttaa
    55261 gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa aaaacgggtg
    55321 atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag gaaaatgtta
    55381 aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt tagggttttc
    55441 ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt tagatgaatc
    55501 taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg acctccctac
    55561 attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac gttttgccta
    55621 acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac aagctacaat
    55681 tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc ctaagcgata
    55741 ggtcgctggt tcaattccag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca
    55801 gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc
    55861 gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt atgtcttagt
    55921 ccatcaataa acagtcgaag agaaaatagg gaaaaattga ttactaaaga aaaacttact
    55981 aggtcgacgg tgtagttgat tttcatactt caatagattc gaattaaacg atttcgattg
    56041 gcttatacca gtcctatcca acgaaagata tatcgttctt aactctagcg gtataacttg
    56101 aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat ttttagtaat
    56161 agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa aaacggtggg
    56221 gttgtttagc gaaataaagg atctaagcca atagcttaga tcctttattc tatgctttat
    56281 attatttttt gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg
    56341 tactgtagag ccatcttcac ggattccgtc gatatccatc ttgtcagatc ctaccatgaa
    56401 atctacgtgg gtttgacttc tattcaatcc agcttctgct aattcttcat cggacatttc
    56461 tgtaccgcct ttcacactga aggcataagc agaacctagt gctaagtgat tcgatgcgtt
    56521 ttcatcaaat aatgtattga aaaagatgat gccagattga gaaataggtg aagggtcagg
    56581 tacaagtgcg acttctccta aacgacgcgc accttcatct gtatcaagca gttttgctaa
    56641 gacatcttct ccttgttcgg cagaaaaatc aacaactttt ccatctttaa aagtaaattt
    56701 catacccgag atgatggtgc cagcataact cagtggcttt gtacttgaaa tgtaaccatc
    56761 tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt
    56821 ttctccacga gcattatagc ttccagcgcc ttcccaaaga tggtttttag gcaatccgat
    56881 aatgatatct gtaccgggag cagtatagtg aagggcagaa aattgttctc gattcaattc
    56941 ttctgctttt tttgctaatt tttcatcatg cttcttccat gctaagacag ggtcttcttc
    57001 atagacacga gtcgttttga aaatctgatc ccataatgcc tcaacttgtt tttcttctgg
    57061 cagttctggg aagacttttg ctgcccattg tttaccagcc gcagccacga cagtccaact
    57121 gactttattt gcttgagttg ccttgcgtaa attcattagt gctttgccat tcgctgattg
    57181 ataagacgct actcgatggc tatctacacc agcaaatgca tcgggatcgg cagaaacaac
    57241 gctgatcctg cttgctcctt tttcaagcca ttcatccgct tgatcgattt tagattgtgg
    57301 tacattctca atacgatccg ttgcagcatg taaaagaaat tctctttgga tctggtcgtc
    57361 agtccattga acgataacct cagcggctcc tagtttataa gcttcttgtg tgatcaatcg
    57421 agcaaggggt gcctgttcca cgctgatctg taaaacaact gtgtgtcctt tttctgtggc
    57481 tacgccagtt tctgcaatta agcgtgcata tttttttaga agttcattaa aatcagataa
    57541 cataataaaa attcctcctc accatttttt ctggtaacta ttagcatgat accatttaat
    57601 tatgattcaa acaatgaggc tttaaataaa aaaaggaggt tattttttta aggttaagtt
    57661 tcatgatata tgttactact tgctatttca gcttgtgaag aaaatcaaaa actaattttg
    57721 catatcaaaa aaacatgtta caatgaaatt aatcaatggt ggaagcgtta taaaaaaatt
    57781 aaggagatta aaaaatatgg caagaaaaaa gacgattact agagaacaga ttttggcagc
    57841 tgcttatgaa gtagctgcca ccgatggatt tacacgattt actgcgagaa acatcgcgaa
    57901 caaaatgaat tgttccacgc aaccgattta tcttgagttt aaaaatatgg atgacttgag
    57961 acaagctctt tttgagcaga tctatgatca tttacaatat gaagtgttca ctgttgagca
    58021 tacagggaat acgattatcg atcttgcttt taattatatc cattttgcaa gaagagagaa
    58081 gaagttgtat cgttcactgt acttagaaga atatggtgga ggaaaagaga tgcagaattt
    58141 ctcttatact tactttgtga atacagtgaa acgcgatcca gcttatgtga atctttcaga
    58201 agagcagatc gaatctttgt ataatgggac atggatcata gctactggga tcgcagcttt
    58261 gatgtcttca agcatcatcc atccaactga tgaacagatt gaaaagatga tccaagacag
    58321 tatagatgct atcttgaaat tcaaagaacc aatcgaaatc gattgaatat actcaaaagt
    58381 tccacaaatc agtaggtaac aagagatgtg tcaaaataag gaaaagttcg tttttcttac
    58441 agcgtatgca tttttaactc ccgaagaact cttccatgaa tgcaatgaat aaaagagcca
    58501 tacagattga ataaatctgg atggctcttt tattgcaagt atattaaatt tttgcgacta
    58561 gttctttagc gaaagcttct aaattttcga tatcttcttc ttcagcattc agatccactt
    58621 taacattttc ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg aaatcatcca
    58681 ccgatttgca gaattcatcg taaaaagtat caccagatcc gcagacaccg taaatttttc
    58741 ccaatagatc caattcttgc aagtcttcat aaaagtctac gatctcatca ggcagatcgc
    58801 catcatcgta tgtataagta gctactacgc aaatatctgc ctcctcgaat tcatctgcat
    58861 ctacttgggt acattcgttg atttcgactt cgatatccag attttcaaat gcttctgcaa
    58921 caatatcggc gatttcttca gtgtttcctg tcatgctagc atatacgatt tttgctaaag
    58981 ccatgatgtg cctcctcgtt tataattatt taacaattat gaattatacc aaaacatcat
    59041 tgaaaagaaa ataaaaccta aagacacctg gggaatgtca tttggctaaa tgtatgccgc
    59101 tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa tatgattact ttaaaatcag
    59161 cacgtgaaat tgaaatgatg gcagaatctg gagctttatt agcagatgta cataaaaatc
    59221 tgcgtgattt tataaaacca ggaatcacaa gttgggatat tgaggtattt gtcagaaact
    59281 atatcgagag tcatggcgga attgctgcgc aaattggctt tgaagggtat gaatatgcga
    59341 cttgtatcag tataaatgat gaaatttgtc atggattccc tcgtaaaaag cctttgaaaa
    59401 acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt tctgattctt
    59461 gttgggccta tgtagttgga gaatctacac ctgagatcga ccgattgatg gaagtaacga
    59521 agaaagcttt gtacatcggt attgagcaag cacaagtagg gaatcggatt ggtgacatcg
    59581 gtcatgctat ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt gattttattg
    59641 gtcatggcgt tggtcctacg attcatgaag aaccagcagt tcctcattac ggagaagctg
    59701 gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg gttaataccg
    59761 gaacttggaa aatgaaaatg gatcctaacg gctggactgc gtatacaaga gacggcggac
    59821 tttcttgcca atatgaacat acattggcta ttactaaaga tggtccaaga attttgacat
    59881 cacaaggaga agaaggcact tattgatcta tccagtcaat aggtacggga gaggtacatg
    59941 aaagtttgga acaaaataaa acaaaatgaa aatctgatgc gctttattga gacgactcaa
    60001 aagcgcatca ctgattcaga aatgagtacg acctctgtcg tcgttgctta ttatttactc
    60061 ttgtctttat ttccattgat tattgctttt ggtaatattt taccgtttct ccacatcgat
    60121 caagagactg tactgaccta tttacgagaa gtgattcctg agaccgttta ccagtttatc
    60181 ggtcctgcaa tcaaagacct tttgacacaa agttcaggag gcttgttatc cgtttctgca
    60241 ttagcggctt tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc gatgaataaa
    60301 gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc actaattgtc
    60361 attgtcttgt tcatgattgc tattagtggg gtaactatcg ttcttgggtt gggacagctg
    60421 attttggatg ctattcagcc aatcgtacaa atacccgtga actttatcga tcagttccaa
    60481 actttgaagt ggccaatcac attggtagct ttatttgtca tcatgttctt gatttattta
    60541 attgtcccta atgctcagtt gaagttgaag acgatcattc cgggcacgat cttcgctact
    60601 gtcggctgga tgctgttatc acaagtgttt agtatctatg ctaggtattt tgcaacgagg
    60661 atcagcggtt atcagatcat aggaagtttt attgtactca tgctttggct taattttgct
    60721 gctacgatca tcattttggg cggaatcatc aatgcagttg ttcaagaata cgtgacgggt
    60781 catcaaatca aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt gaaaactaaa
    60841 ttttctcata aaaaaaagta gaaacaagag ttcttattgt aaatttatat cagtaattta
    60901 taataattat gactcttaat taaagtaggt gcccatatgc ttgtttcttt tagtatggtt
    60961 ataagatttt ttctgacaat tttattatca ctgatattaa cacctgtatt caaaatcatt
    61021 tcagtacaaa caggtatggt cgacaaacca aatgaaagaa gaataaataa agttccaatg
    61081 ccttcagcgg gcggactgcc tatctttatt tcatttgtta tatcgacttt attcttattc
    61141 cgtaaaatta tccctcaatc gtatattgta ccgatcttga ttgcttcttt agtaatcata
    61201 gtcacaggag tgctcgatga taaatatgag ctgacaccta aacaaaaaag tatagggatc
    61261 ttgattgctt ctcttattat ttattttgta gcagatatac gtattgattc cttcacgctt
    61321 ccttttattg gatatatcca actaggctgg cttagttttc ccgtaacgat tttttggatc
    61381 ttcggtatca cgaatgcggt caatctgatt gacggtttgg atgggctggc ggcagggatt
    61441 tcattgatcg gtttaattac gataggaatc attggatatt ttttcctcca tgcttctaca
    61501 gtgtatattc cagttgttat tttctgtctt gtagcaagta ttatcggttt ttttccttac
    61561 aatttttatc cggcaaagat ttatttaggc gatacaggag ctctattcct agggtttatg
    61621 atggccgtcc tttcactaca agggctgaag aacgtgactt ttgtttcatc gatttctttg
    61681 ctgatcgtca tgggagtgcc ggtaacagac acgttttttg ctatcattag gagaaaagca
    61741 aaccgtgttt ctttttctac agcagataaa aaacatcttc atcaccgact aatcagttta
    61801 ggctttactc ataaaggtgc tgtcctgacg atttatgcga tggctttgat gttttctttc
    61861 acagctatgg tcatgaatta tactggaaga atcggaacga tcgtattgat tattgccatg
    61921 ttgtttgctg caattttatt atttgaattg attggtctga tcaatgagaa acatcagttt
    61981 attttaaaaa cattgcgatt tttagggaat aaagagtacc gtacgcaagt aaaacagaaa
    62041 tatttgaaga aatggaaccg tcatatagat gaataacaaa ttaaaccagc cgatccttat
    62101 tagtattgtg acggcaaaca gtgataaaat ttttcggaca ctagataatt ttatttcaac
    62161 tgtaaaagat gaaacagcag taaaaatccg aatttttgac aacaattcac ttcccgagta
    62221 caaaaaaaga ctaacagagt ataggaaata tccctacatc gatatctatt ttcatgatga
    62281 aaattccgga tttggttatg gacataatca caatctgata gctagcgatt ctcgctatgg
    62341 cattatttgt aacccagata ttttagtgga tgaagagaca gttacagcat tggtcttgtt
    62401 attgaaagaa catccggaat gtgcgatgtt agcacctaag attcttaatg aagatggaac
    62461 aactcagcac ctcatccgtg agcagctggc tgtttttgat tacgttttgc gctttgtgcc
    62521 tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat gccgaggatt
    62581 gcctactgat gctaatagtt atgtacggat gatctcaggt agttttatgg tagctgatat
    62641 tgataaattt aaagagataa aggggtttga tgagcgtttc ttcatgtact ttgaagacaa
    62701 cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta ttgtacacac ccttttatgc
    62761 agttatccat ttttacggga agggtgcgca tcgaaatgca aaactgtttc gcgtgttttt
    62821 gacttctatg atgaaatttt tcaataagtg ggggtggcgt tttttctgat gaaaccagaa
    62881 atattagtag tcgttgtgct ttatcagcaa ctattcagtc aatctccagc ttatggacca
    62941 ttaagcaaag cacttagcga aaagcaaatt cagcttatta tttacgataa tagtcctcaa
    63001 aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt atcatcatga tccgcaaaat
    63061 cctggtttag cggtggctta taattttgcg ttgtctcaag cagatgaagg aacacgttgt
    63121 ctcgtcactc tggatcagga tacgaggcta gtcgacgatt attttgaact attgcgtaaa
    63181 gtaaccttta cagatgaatg tgttgctgca gttccgatga ttttttctgg gagccgacaa
    63241 gtttctcccc tatattcaga tcgttatatc aatcgtacag ctcaagcagt agaagttgaa
    63301 ggcccaacag ctgaacgaat catggcaatc aattcaggtg ttgcctggtc tgtcaaattt
    63361 ctaaaaaaga ttggcgggtt taatcctgct ttttctttag attttttgga tcattggctg
    63421 ttttggaaag tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg aatggaacat
    63481 gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc gataccgttc tattttacaa
    63541 gcagaaaatc gattttatca tgaatatgat caaaggcatt tatccagtca ccgacgacaa
    63601 ttattgttaa gaacagctaa acagttcttg accgtcaaaa accggcatat ctggcggatg
    63661 actttgcgct catatgttaa gaattggaag gtgtgaattc gtgcgttcgg tttgtatagc
    63721 tacctttaat ggaggcagat atttaagaga gcagttagat agtattttat ctcagctttc
    63781 agcagaagat gaagtagtca tttctgatga tgggtcagta gatgatacat gggatatatt
    63841 aactgaatac gcagaaaaag ataagcgaat ccagcttttt tcggggcctc gtcaaggatt
    63901 gatcgctaat tttggttatg cgatccagca gacgaaagga gatctaatct ttttggcaga
    63961 ccaagatgat gtttggttgg agacaaaagt gaaaaagatc actgactatt ttgagcagaa
    64021 tccggatcag ctagtagtag tttctgattt aatcattgtg gacagcgagc tgcaagagat
    64081 ccatgattcg tatttttcgt ttcgaaattc caaagaagga tggcttaata atgtgataag
    64141 aagtcactat attggtgctg gtatggcttt ccggtcttct ttaaaacccg atattttgcc
    64201 gatcccgtca gaagtgccga tgcatgatat gtggattggt ttgctgggag gaaagaagac
    64261 tgggttccta agagaaccgt taactctgta ccgaagacat gatttaaacg cgagtgagat
    64321 tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt gttcatctga ttacggcgtt
    64381 gatcaaacga aaatggttta aacgttgata aaagatgttt aaatccttaa tatttccatg
    64441 acaaatataa caattgatga gataataaca aagtgaaatt tgaaatcaaa aaggagagca
    64501 gatatccatg aagggaatca ttcttgctgg gggcagcgga acacgtctgt accctttaac
    64561 aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt attatccaat
    64621 gtctactctg atgttggcgg gaatcaaaga aattctgatt atttcaacgc cacaagatac
    64681 accacgtttc aaggaattat ttggaaacgg aaatgattta ggactgcaga tcgaatatgc
    64741 agtacaagaa agtccagatg gattggcaca agcgtttatc attggagaag aatttattgg
    64801 agatgatagt gtctgcttgg tattagggga caatatttac tatggtggcg gtctgtctaa
    64861 aatgttgcaa cgagcagctt caaaagatac aggcgcgaca gtatttggct atcatgtcaa
    64921 tgatccagaa cgctttggtg tagtagaatt tgatgagaat atgcaagctt tgtcgattga
    64981 agaaaagcct gctcagccaa aatcaaatta cgctgtaact ggcctttatt tttatgacaa
    65041 tgaggtaatt tctatcgcta aaggtatcaa accttcagaa cgtggagaac tagagattac
    65101 ggatgtcaat aaagcatatt tggataaagg aaaactatcc gtagaactta tgggacgcgg
    65161 atttgcttgg cttgatacag gaacacatga atcattgctt gaagcatcta cctttatcga
    65221 aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg cttaccgtat
    65281 gggctacatc gataaggaac aattgctggc ccttgcacag ccattgaaaa aaaatcaata
    65341 cggtcaatat ttattgaatt tggccgctga ataaggagtc cttacagatg aaagtaacag
    65401 atacaaaatt aacagatgtc aaaatcgtag aaatggacgt ctttggcgat catcgcggtt
    65461 tcttcacaga aagttattca aaagcaaaat ttgcagaaca tggattgaaa tatgactttg
    65521 ttcaagacaa ccattcacta tcagctgaag ctggtgtcct tcgggggctt catttccaaa
    65581 aaggagaagc tgcacagaca aaattgattc gggtagtcac tggagcagtt ttagatgtag
    65641 tagtggatat ccgcaaaggt agcccgactt atggtcaatg ggacggattt atcttatctg
    65701 aacataatca tcgacagttg cttgtaccta aaggatatgc ccatggattc gttactttga
    65761 caccaaatgt gaacttcctt tataagtgtg ataattacta taatgcagaa gcagatgggg
    65821 gcatcgcatt tgatgatcca gatttagcga ttgattggcc aattgatcca tcaagagcga
    65881 ttatgtcgga aaaagatcac cgtcatccga cattaaaaga atttgaagca gaaaatccat
    65941 ttgtttacgg agaaatctaa gaattaagga gaaattttct tatgaaaaac atcattgtta
    66001 ccggaggtgc cggattcatc ggctctaatt tcgttcatta cgtggtaaac aatcatccag
    66061 aagtgcatgt cactgttttg gataaactga cttatgcagg aaacaaagag aacttggccg
    66121 gacttcctag cgatcgcgta gaattggtcg taggagatgt tgcagatgca gaattagtgg
    66181 accgtctagt aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc cataacgata
    66241 attcattaaa agatcctttc ccatttgtac aaacgaatat cattggtacg tatacacttt
    66301 tagaagcttg ccgcaagtat gatgtacgct atcatcacgt atctactgac gaggtttacg
    66361 gcgatcttcc attacgtgaa gacctgccag gacatgggga aggcgaagga gaaaaattca
    66421 ctgcggaaac accatataat ccttcaagtc catattcttc tacaaaagct ggttcagatc
    66481 tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc aaccatttca aactgttcaa
    66541 acaactatgg tccttatcag catattgaaa aatttattcc tcgccaagta acgagtatct
    66601 taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac tggatccata
    66661 cgaatgatca ttcttcagca gtgtggctga ttttgacgaa aggcagaatt ggcgaaacct
    66721 atctgatcgg tgcagacggt gaagaagata ataagaccgt gatggaattg atcttagaaa
    66781 tgatgggcca accaaaagac gcatacgacc atgtaaacga ccgcgcaggg cacgatttgc
    66841 gttatgcaat cgattcgaca aaattgcgag aagagctagg ttggcagcct gagtttacga
    66901 acttccgtga tgggttggct gacacgatca aatggtacga agaacatgaa gactggtgga
    66961 aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa tggacaataa tacagaggaa
    67021 atttcactgt attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc tcttttttaa
    67081 aataaaaatc aaggagcgga taaccatatg tttttattaa ctggaggaaa tggacaatta
    67141 ggtacagaat tgcgccattt attagatgaa aaaaatctgc catatgtttc aacagatgca
    67201 aaagacttgg atatcacaga tgctgagaaa acaatggctt atattacaga attaaaaccc
    67261 gatgttattt tccattgcgc agcttacaca gcagtagata aagcagaaga agaagcaaaa
    67321 gaattagatg agaaaatcaa tgtcgatggc acaagaaatg tagcacttgc agcaaaagct
    67381 gtgaatgcac aattcgtcta tatcagtaca gactatgttt tcgatggaaa aaacaaaaat
    67441 gtagaatacg aagtagacga ccaaacgaat ccactaaatg aatatggtcg tacgaaatta
    67501 ttaggcgaaa aagcagtaga agagatttta gaagactatt atatcatacg aacttcttgg
    67561 gttttcggga tctatggaca caatttcgtc ttcacgatgc aacgcctagc ggaaacccgc
    67621 gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg tactttggca
    67681 gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg gtatttatca tttgtcgaac
    67741 gaaaatagct gttcttggta tgagttcgca aaagaaatat tgaaagatac agatgtagaa
    67801 gttgcacctg ttacgtcaga agaatttccg caaaaagcaa cacgtccgca gtattcagtc
    67861 atgagcctga aaaagacaga agcgttaggt tttgtcatac caacttggca agaagcactt
    67921 gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat ttccttttta tcaaatagaa
    67981 tttggagcat gatatggaaa aacaaacaag aagtgcacga cgaaaagaaa agccaaaagc
    68041 gaatcaggca gattcgaatt tggtgcttga cctgtttcta aaactccgct atcttattgg
    68101 cgttttagtc atcgtggtca tcgttacttt taatttaaac ggaagttcac ttggtgtttg
    68161 ggataaatat gtgtcgcaaa gagacgatgg gaaaaaaact gatgtgattt ttggacagaa
    68221 tcgggaagtg cggtcagacg aatggctggt gcagacacct ttttacctct cacaagctga
    68281 agaggacttt tcgttggtga acgaggatta ttcattaaac ggacaaaaca tgatcattgc
    68341 gtataattct cctgtaaagg atattacggt tatcggaaaa ccattcaact ggggattctt
    68401 tttcctcgga agagatcgag gactttcctt ttattgggcg atgaaattag tcgtcatggt
    68461 cttgcttgga ttcgaagtac tgatgatatt aacgaagagg aaaaaagctc tatcgctaat
    68521 cggtgcattt gcgctgacat tttctccagc agtccagtgg tggttcatgc aacatgtggg
    68581 cgatttgatt ttctttactt tgggactgat gattgctttt tatcattatt tctacaatca
    68641 tgataaaaaa tggctacgaa cgttgatgat gcttttggtg gttattttcg gtattgggtt
    68701 tattttagtg atttacccag cccatcaagt gatgctggcg tacttgctga ttttttattt
    68761 tgttggcctg cttatttact atagcaagaa agtttcatgg gatctgttgg atgtagtgtt
    68821 gattggaggg gcagttttat ttattggcgg agtcatggtt catttctggc taacgtcaaa
    68881 agacgcattg ttagcttctt tgaatacctt atatccagga aaacgggtaa gtactggagg
    68941 aaagtggacg attggcgaat ttttttcttt ccttacaaat tggaaaattc cttttgaaga
    69001 tatcaactat tcgaataact ctgaagtggc cctgttctat cacttttttc caactgtatt
    69061 cctagcaagt ccatttgtgt tatttggcaa aaaagatagc gaacaaaagt tactgggacg
    69121 tattttgatg attttttgtc tgttttctat cttttggatt accgttgggt tgccaaaatg
    69181 gctagcagaa atcactctgc tgtcttatgt tcctccatta agagccatcc ttacttttag
    69241 ttttgcggct tgtttattat cgatctggtt tatcgcttat atttggagag aagatgttat
    69301 tcctatttgg tataaattca ttttgttggc agtaaatgca tggtcttatt tctatgcgat
    69361 tacgcattcg aaaatggata attatttttc ggattttgaa accatcgcag ttgctgtgtt
    69421 gggaacagtg attttagctt ttgcattatt tggctggaga cgcttgtttt acctcgcttt
    69481 tgtcgcctta gttatcgtat ctggcttttt cgtcaatcca gtcgtagaag gtacaggagc
    69541 gatttttgaa aaaacactgg caaaagaaat ccaaaaaatc gatcaacaag atcctggtaa
    69601 agtttggttg tctgaagatg aattatataa cttcacccca acattgggag tgaaatcctt
    69661 caatacagta cgcttttatc cagatatagc tgcatggaag aaaatcgatc cgaaagaaga
    69721 atacgagaag tattataatc ggtatgctca tacaagagct tttatcgcta aggaagatac
    69781 aaagttcatt ttagatcgtc cagatatgtt ttcggtcacg ttgaattttg aagacgcaga
    69841 aaaattaggg atcgactatg tgatcagtaa acgaccatta actgattaca accaattata
    69901 tgaagcgcag ttcacgcagc tatatggacc ggataaagac ggttatatga tttttaaagt
    69961 agaatatccg gattatccag atgtaccaca aacacaaact caggaaaatc agctagtcgt
    70021 acagtaatag gctagctgag aataatcctg aaatattagt agaaaggaga gtgaagataa
    70081 ttcaagcaat cgtacaaaaa tttgtcaaca aaatgagtgt cggtttcttg cttttgttgc
    70141 tgacgctagt cttttttgtt tggacagcag gggtaggttt tccagcaact ttgtttatat
    70201 ataacaatag ttcattactt atcgctttta gtgtggctat tttgctcatt ttaaatgttt
    70261 ataaagcaaa aggcgtcgat tttctttatc taggtgttgc gcttgccctt ttcattttct
    70321 tttcttactt tcgggaatta agaaatgagt ctttatttgg agacttactg tttcctttgg
    70381 ttattttatt ttgtttagta attaaatgga tcgagtggac aaaatgggat cgcggtattt
    70441 atctgattgt ctttacggtc ttcctcggaa tcacgttata tcgtgtattt acagagatcc
    70501 aagtaccaga aggacaaagc atctgggctc caggcaattc gctttctgat atttggatca
    70561 atacgaatac aatcggaagt tctttgatga ctcttgcttt attgatcagt ggatttgcca
    70621 gttcttttga acgatggtat gttcgaatca tcggtgggat agcagtcgtt gcagcctttt
    70681 taggtatttg ggtctgccaa tctagaggtg cattagtcgc gttgattgtt tttgcgatat
    70741 tggatctttt acctaaatcg ttgatgcgtg tcattcgtgc gccatttatt gcctacacgg
    70801 ttacgattct tttagctttg ccgatttctt atctggcggc tgtctctgaa aaagtcaatt
    70861 tgtttaccga tcgagaagat atttggcata aattctatca gacgctagga gaaaaatctg
    70921 aacaagtttt actaggaatg aaaacattta tcttccagcg aggaaaccaa tttttaggaa
    70981 accacaatag ttataattct attttgaata tctatggatt gataggattt gggattgctg
    71041 ctttgctgct tatccttttt attggtcgct taacgttgaa agcagattta tccaacgggc
    71101 agatgacttt tatctgggcc ttttttgcgg tgatgatgca gtcatttatg gaggatacat
    71161 tgacttcata cccatgggtt ccgattattt atatcctgtt ggctatggcc gttcatcgat
    71221 atgatgaacc agaagttgcg gaggaattct atgttatgga agaggcatat gacgatatga
    71281 cttatgatga tacatcttat gaggaacaac cagatagcgc tcattcttcc aggatccaac
    71341 gctatcattg ataggcacaa aatacgagag aggctgtgac aaccgtcgca gccttttttg
    71401 gtttattgca attgtctagc atcttctgta aaatagaaac gttagagaaa atcttgtatc
    71461 atagaaaatt aagaaagaaa taagaacgca gctcggtacc aaaaatatgg taaaatggca
    71521 cttgactgta attcactgaa aatcgcttgg aggaacacaa atgtttaaag atttgtattt
    71581 atttatgaag gacatttatc agaacagaaa attattgcta cagttttcga tcaatgactt
    71641 caaatcaaga tatgcaggat cgtttttagg aatcttatgg gcgtttatca atccgctatt
    71701 tactgtattg atttattggc tggtatttgg tgttggactg aaagcacaaa tgatcgatgc
    71761 aaaataccca tttatcgtat atttagttac cgggatgatt ccctggttct tcttctcaga
    71821 tgcatttgca tcaactacgg ttgtttttcg ggaatatact tatttagtaa agaaagtcgt
    71881 attcaatatt cgtatcttgc ccacgacgaa aatcctatcc aatctgtaca cccatttatt
    71941 cttcattctg attggcatag ctgtcgcgct tataaatggt atatatcctt cagcaatgtc
    72001 tttacagctg atttattatt tattttgcat gtgtgctttc ttgacaggat tgacttggct
    72061 gacggcatcg atccaaccat ttttacctga tatcatgcaa ttaattacga tcgtgctgca
    72121 gttgatcatg tggacattac cgatattatg gagccctaat cagtttgatt caggtcttgt
    72181 aagcatattg aaattaaatc cgctgtacta tgttgttcaa ggttatcgtg agtcattttt
    72241 aagtgaagct tggttctggg agcatggaac atatactttg tatttctggg ttttcacgtt
    72301 agtaatgttc gtaatcggct ctgcagtgtt ccgtagattg aaaccacatt tttcagatgt
    72361 gctgtaagga gaatagctat gacagaatat gcaattagac taaaagatat cacaaaatct
    72421 tataatatgt acgcaaaacc ttcggatcga tttcgtgaag ccctcaatcc ttttaagaaa
    72481 tcctatcatg atgtatttta tgcgttgaaa aacgtgaatg tagaaataaa aaaaggtgag
    72541 atgatcggtt tcgtgggaga aaacggatcg ggaaaatcta caatgttgaa aatcattacg
    72601 ggagtactga caccaacatc cggcacgatg gaaatcgaag gaaaaatctc agctttgcta
    72661 gaactaggat caggattcaa tcctgagtat tctggctatg aaaatatcta tttaaatgga
    72721 atggttctag gattttcaag agaagaagtc gatgaaatgg tcgatgatat cattgaattt
    72781 gctgacatcg gagaccatat ttatcaacct gtaaaaactt attcaagcgg gatgttcgtt
    72841 cgtctagctt ttgctgtagc aatcaatgtg aatcccgata ttttgattgt ggatgaagca
    72901 ttggcagtag gcgatcttga atttcaacta aaatgtatgg aaaaatttac agaaatcaag
    72961 aactcgggaa agacgatttt attcgtttct catgatatca gttcgattcg tcgtttttgt
    73021 gatcgttcct attggttgaa aaacggagaa gtcgtcgaat atggcgatac gatggaagta
    73081 acgacgaatt atgaaaactt tttaaagaaa aaatcagtta aaacagtgga tcggaataaa
    73141 aaccaagtag aacggttcgc cgctccagat atcgtcgacg tgacgaaagc ggaattgttg
    73201 gataaagaca aaaatccggt ggatatgctg gaacaaggag aaaaactata cgttaaagta
    73261 acttatgaag taaaagatga tacaatcaaa aatccggttt taggaattgc gctacgaaca
    73321 gtcgacaatt tttatgtatg cggacttaat actctactag atggtatcaa gattccttgg
    73381 aaaaaaggag agaatatctt ttatctggag tatgaaaagt taggtttgtt agctggtgaa
    73441 tattattttg acgttgcggt ctttgaagaa aatgcaacgg ttcctttggt atataagaca
    73501 aagtacatga acttgtttgt cagcggttct tatatcggag aaggcatcgt ggtcttagac
    73561 cataaatggg aagaagggac tcatagcaat gaaatatgat tttgaaatgg acctagatga
    73621 acaaagcagt gtgggaaaaa ttgccgcaca gatcaaacca ggaagtaaag ttttagagtt
    73681 tggtcccgga aacggccgat taacgaaaca tttaatcgga gcaaaacaat gtgaagtgag
    73741 cattgttgag ttagataagg aattgttcga ttttgtcagc gaatttgctc aagatggatt
    73801 ttatggtgat atcgaaagtt ttgagtgggc aaactattat gctggacaga catttgatta
    73861 cgtcttgttt gcggatgttt tagaacattt ggtagatcct ggaaaaacat tgaagaaagt
    73921 cagagaattt ttgaatgaag aaggagagat cctgattaca ttccctaact tggcgcataa
    73981 ttctgtcatg atcgatttgt tcaataacca attgccgtgg gcttcttatg gtctcctaga
    74041 tgaaacccat aattcttttt atactcatga tggcttccaa aaagtttttg aaaaagcggg
    74101 attattcatt aatattgaag attatttgta cttagctgta ggtgatacag aattaaaatc
    74161 aacttatgaa gaattgccgg aagctgtacg ctatgatttc aaaatgcgac catttggtga
    74221 agtctaccaa tactttttct cattgatgaa acatccagta gcacaaagtt ccattgcaga
    74281 gccacaaaat tctaattatg taaaagtgtt agaagtaacc cagcaaacga aacaagatga
    74341 aacaatccaa caaataccat tcaataattt cacaggggaa aatgagacac taagtttccc
    74401 cgtatcagaa acaacagaaa gaatggtgtt tcgcttcgcg caacaaccta gctttatcga
    74461 attcagcgca gaagctgccg gagaaaaact aacatttatc gattcgaatg ctgtagtcaa
    74521 aacagtaaat gattgttatt tgtttgatgg gaaagaatta ccagagttcg ttctaacgga
    74581 tatttctggg aaagaagtga ctatccattg tcactatcgc tttatcgggg aattgacacc
    74641 aacaatgaaa gaattgttgg aaacgattcg tcccatggct gaagtcacaa agcagctttc
    74701 tgagaaaaat gacgaattag aaagagaaaa tcatcatctt cttgaggaaa atacaagact
    74761 agaccatacg ttaaaaacaa ccacaaatcg ctattgcaca ttgttggaaa gtgatgaatg
    74821 gacaataaaa catagactag gacgacgtaa gaaagaaact tcgaaaaaaa tccaagaaaa
    74881 agaactttct atatgtattg acgagaaaat ctgggatgca gaaacaaaaa tattaaaaat
    74941 catcggatgg ggaatcgcta attcggatcg ccagcccttg tcctataagt tatcagctga
    75001 ccaatcgcca tttttcgaag caattcctgt ttcacgagaa gaagtcaatc aagcagaaca
    75061 gctcgcaaaa ggaacagaag cgggatttga gttacgaatc ctttgcgagc aagaacgttc
    75121 attcttagtg gaagcagtag ctcaaaatgg ccaatcatgg atcattgaaa tgtaaataat
    75181 gattaactta taaagataag gagtaaacga atcgtgcaaa aaaaagaaat tgccgtattt
    75241 atcgatcaaa tcactcgtga acgggccact ggcgatttgc ttattgtggg ctgggcaatt
    75301 gatgaagtga ccaaagaaat ccctacgata aaggtagaaa aagaaaatgt gattgctgaa
    75361 gcaacacatg tcgtaagatt ggatatcaat catttatata atcttgatgt taagacccaa
    75421 agcggtttca aggtacgttt atcagggaaa atgagaggaa aagccatctt agacttccaa
    75481 acagcgagac atcaaaatgg catagctgtg aaattaaacg gtaaataccc ttatgatgac
    75541 ggcattgaat cttcatggga aagaaaaaaa agattactga aaaaagggat aaattatgca
    75601 aggactcatg gcgtaaaaaa agcgatccgt cgggtcaaat tggaattgaa gccaggatcg
    75661 atcgactatg cagagtggat cagtcgtcat gagaaaccag atttgaaaga gcaaaggaaa
    75721 gaagcacaag gatttgcgta ccgtccattg atttccatac ttatgcctgt ctataacgtt
    75781 gaaatcaaat ggctcgaaaa atgcattgat tcggtactgg atcaaacgta tgatcattgg
    75841 gaactatgta tcagtgatga tgcttctact gatccagcta tccgaaagtg tctggaatcc
    75901 tatcaagcaa aagatgatag aatcaaagtc gttttccgaa aagaaaatgg acacatcagt
    75961 ctggcaacta attctgcatt ggaaatagca gaaggagaat ttatagcact cttagataat
    76021 gatgatgaat taccgccatt tgcattatat gaagtggcaa aagtgctaaa cgtacatcca
    76081 gaactggatg tgatctacag tgacgaagat aaaatcgatg cagatggcaa tcgctttgat
    76141 ccgcatttca aagcagactg gtcgcctgat acattgatgg gaaataatta tatctctcac
    76201 ttaggcgtat atcgaacaag cattgttaaa gaacttggcg gtttccgtaa aggctatgaa
    76261 ggttctcaag attatgattt ggtccttcgt gtgacagaac aaatccctgc agatcatatt
    76321 taccacattg accgagtctt gtatcattgg cggacgatcc caggatcaac tgcaagtaat
    76381 ggcgaagcca aaagttatat ctatgattcc ggagtgaaag cattgacaga tgccctgtct
    76441 agaagaaaca tcaaaggaag cgtacgtcct ggcagaattt ctggatttta tgagattgct
    76501 tatgacgtct tgcaagagga tttggtcagt gtgatcattc caacgaagaa tggctatgaa
    76561 gatctaaaaa cttgtgtgga ttcgatcatc gaaaaaacgt cttattcaaa ttatgaaatc
    76621 attattgcgg ataacggaag tacagatcca aaaatgcaag aattatttgc agaatacaag
    76681 catcagctga aagaccgctt tatcgtagaa ttgattgaca ttccattcaa ttattctcga
    76741 attaacaatt tagcagcaga aaaagcaaac ggcaaatact ttttattctt gaataacgat
    76801 acagaagtga ttgagccaga ttggatgacg gtgatggttt cttatgcaca gtttgatcga
    76861 atcggctgtg taggagcaaa actgttttat ccagatgata caacgcagca tgcaggagta
    76921 ttattaggaa tcggtggtgt tgcagggcat gcattgaata actatgatcg aacacattgt
    76981 ggttatttcg gccgattggt cattgacgtc aattacttag cagtcactgc agcttgtatg
    77041 atggtcaagg cagctgactt caatgctgtt aacgggtttg atgaaacatt agaagtagca
    77101 ttcaacgatg ttgacttgtg cttgaaagtt tatgaactgg gtcgttacaa tgtctatgca
    77161 catcaagcag aattgtacca tttcgaatct aaatcaagag gttatgaaga tactccagaa
    77221 aaacaaagac gttttgctgg tgaaatcaaa aaaatgcaag ataaatggcc agcatatatt
    77281 gcgcatgatc cattttacaa tgataattta acaaaagacg gaatcggcga cttttcgttg
    77341 agaccagatt aaactataat aaaaagctgt tatgttgtga ttcgctcgca cataacggct
    77401 ttttatagtt ttttgtcaaa aaaaggcaga aggtgccgac tcaaaaaaat aagtcgaggt
    77461 tgggacagaa gtgtttaact ccgagaaata agaagaaatt cacgaaaatt gcttttcaaa
    77521 tttttgtgaa tttcggctta tttaagaaga agttgcttct gctcccaccg tttattcgtt
    77581 tttaaagaat gagacaaaag tgtttcttac ttttgtccca cgctcttatt catgctcaga
    77641 gcgccaatag atacaaaatc atagccaaag tttcacgaac cctagctgtc aaagaatcga
    77701 tattttattt taatataggc tattttaaaa taaatggttt aaggtattcc tctacaaaag
    77761 ccactctatc aattatcatg ggtttaaaat aagaagtgat ggagataacc agattgttct
    77821 tagcgtcaat ataaagaaca tttccactat taccaattgc tgaatatatt tcgttctcag
    77881 aatctaatac ccaccataaa tagccatatc tcatattttg taagtcttct aaagcactgg
    77941 ttctaggttt gaacatctcg tttatccaca tgggtggtac gatttgtttt cctttataca
    78001 tccctttatt taggcataat acaccaattt ttgccagatc acttgccgat aaacacaaac
    78061 catatcctgc cgtcccgata tttagtggat cacaaaacca gacattggtt ttaggagact
    78121 tatccattgt aaactgctta tgttcttcag ctgtttcagc ataataattg acatggtttg
    78181 gtatccctat aggatcaaat agatacttat tggcaaaatc aacagttgtt agcttacttg
    78241 tctgatacaa caagccagtt aatatgtgta aacagacagt ttgatagttg aattcgccgg
    78301 ttataccttt tcttcctccc aataaatcta gtgatgtttt tgtccaatct tcacttgaac
    78361 agacttttgt ccatggatcg cctttacatt tgtagggtgc tttcattgtt agcaagtgct
    78421 tgattgtaat agcgtaaata gttttctccc cgcgtttcac tttatactct ggaaaataat
    78481 ctaatacttt atcatcgata cttttgatta atccttgctc taaagcaatg ccaatcagca
    78541 aagatacaat acttttcgtt gctgacatga tatgacaagt atcgtctttt ttataaccat
    78601 tccattcatc tgtataaact tctaccccat ccttatatgc gacaatttga catatatttg
    78661 gttgtcgaaa accaataaaa tcatgcaact cttgtttatt cataagatat tacctcactt
    78721 tcttctataa gcataatgtc attaaaaatc gatttcaatt ttttcgattc ttcacgtaat
    78781 acaggtattc ttatggacta gacagcgctt tgggaagatg gattctctga aaaatataaa
    78841 ggttcacttt atgagtggat aaaaaaagca gcaaatactt cgacttcttt aatttaccaa
    78901 cattagtgaa aatcgattca tctggagatt tgtaatttag agtccttgtc ttgattagag
    78961 gtgtataaag ctttttatta gacattttta aattccatta caatttgctt ataaactagg
    79021 tttttaaagg tgactatggt aagataatag agatattatt ttgtgattag gagtgtatat
    79081 atgaataaaa atggagaatg gagtgctgca cgacggatat tcattatatt aatggatgtt
    79141 attgtataca atgcagcaat atatagtagt ttcttactga agtttcgtgg agagatccca
    79201 ttacgaaatt tagaaacttt ccaacactca gcaattatta tttcagtgat ttttattgcg
    79261 ttgaacattt tgttgggtgc atatgttttt tataatcgaa tgatcagtga catcgtgttt
    79321 gtcacagtca tcggtcaggt attgatgaca ctagggatca tggtagtgac ttttgcgggc
    79381 cgctggtttg cttttccacg tcttgttatc ttactttcat ttgttctcgg tacgcttttc
    79441 ttaagtatct atcgtattct cgtatataaa ctttatttac gcgtaagtcg agataaaaag
    79501 gtcgtcatta ttgggttaga aaaggatgtc gcaccagcaa tccaaaattt tggttcaaag
    79561 aaaaacaaca aacataaagt cgaagcagtc attcttgata actattatgc aaacatcaaa
    79621 aatatgatta aagacatcga tattgtctat cttgcttctc atatcgaaga aactgaaaaa
    79681 atcaaaatct atcaattagt tactaaaaat gaaaaaaaat tgtttttgaa tacaacattt
    79741 gaaaacttga cattgatcaa tccaaatatt atgaatttcg aagatgaaag tattattgaa
    79801 gcatctggat ttagaatcca acctgaatat gaactattca aacgtttgtt tgatatcatc
    79861 gtttcacttg ttttattagt tattgcttca ccatttatgt tattcacagc aattttagtc
    79921 aaagtgacat caccgggacc aatcatctat aaacaagtgc ggatcacaaa aaatcaaaag
    79981 gaattttcaa tctataaatt ccgctcgatg accgccacag ccgaagcaaa atctggacca
    80041 gttttagcta agagcaatga tgcacgagta acgcctgtag gtaaatttat tcgagctgtc
    80101 cgctttgatg aattaccaca aatcttcaat gtgctgaaag gcgatatgtc gatcgtagga
    80161 ccacgtcctg aacgttcttt cttcgtcgat cagttcaatg cagaaaaccc ttattactac
    80221 ttacggcata atgttcgtgc tggtatcact gggtatgcac aagtctatgg aaaatatgtc
    80281 tctgattaca acagtaaatt acgttttgat ttactttata tcaaaaaata ttcattgatg
    80341 cttgatttaa agatcttgct gcaaaccatc aagatcctat ttgataaaat gtcctctcaa
    80401 ggacttgaag aagacgaaaa tgtgatctca ttggcagaaa ttgcttttcc gaatgaaaaa
    80461 atctttcgct agttgggagg cgtaaaaatg aacaatccga aattcagcat aatcgtacca
    80521 gtatacaatg cagaagcaac tattggtcga acgattgata ttctaaaaga attggattat
    80581 gaagagtacg aagtcgtttt agtaaatgat ggatcaacag atcaaacaca ggaaatcatt
    80641 caagaactga ttgaaaatga tgaacgtttt gaattgatta ctacgaccaa tcaagggccc
    80701 ggattagcaa gaaacgaagg aataagacag tcaaaaggag aatatctctt attcttcgat
    80761 gcagatgaca cgccagtaaa aacgatcttg tcagattatg atcatttgct agaaaagagt
    80821 ccaaaagcgg atcttgttgt tagttcgttt gtttttcgta caatggacga gggaagaatc
    80881 gtttccgaaa aagaaaactt agttacagaa tgtcactatc taactcacga agagtttata
    80941 aaagacatgt atcagttaat gaatgaacag ttgatgtatg ttgtatggaa caaatgctat
    81001 cgtagcgata ttctgaaaaa taaccaaatt ctatttaaaa cttataatag ttgtgaggat
    81061 cgaatattta acttacatta ttatgaatac tgtcatcaag tgatcatgaa cccaaaagta
    81121 gaatatattt atgaatttga aggtgggaag gggatcacta atcaataccg acctaacaag
    81181 ttcgaaacat ttaaagaatt ctattcatta gcaaatactg taacagacga tattaataaa
    81241 gacggtatgg cggcgcttct tttaaaaggg acaacttcag ttattttttc tatttatagt
    81301 acctctgcaa gaacttcaaa agagaagaaa gacgaagcaa agcagatatt gagtgatcca
    81361 gtgatccgag aagccaagca aattgctaag acggattctt cagcaaaaaa aataacaaag
    81421 ttgctttaca atatgccagc tccactattt ttatctgctg taaaagttgg aagcttcgtt
    81481 gaagtgaaaa tgccagggtt aatggctgct ttgaagcgag tatattagtt aatggaggaa
    81541 aatataatga aaagaatttt gcattttcag ggacgaatgg gtttaggtgg agcagagtct
    81601 ttcatgatga atttatatcg taaaattgat cgtacaaact atcaatttga ttttttgatt
    81661 tatgaagatt atgcagatgt tcaagactat cattcagaga ttgaaagatt aggaggccgt
    81721 atttttgttg tacctaatcc taagaaaaat atttttaaat atttaataga agtgaataag
    81781 ttactaaaaa aagaatcttt ttctatagtt cacaatcagg tttattttgg cgggggaatt
    81841 aatttatggt tagccaaaaa gaatggaatc agacagagaa ttgctcatag ccatgcaact
    81901 gaagatggaa aatctcaaaa tattgtaatg aatgtattgc gtaaattttt aaccaaattg
    81961 ttgttacaaa atgcaacaga ctaccttgcc gttagtcaag aagctggtga aagtctgttt
    82021 caaaaccatc cctttgagat agttcataat ggtattgatt tagagcttta tagtaaaaat
    82081 tcagaagcta aggttaataa gagaaaagaa ttggatatct caatgagtac ttttgttgtg
    82141 ggaaacattg gacgattaga gaaacaaaaa aatcaatctt atttgttaga gattttttta
    82201 gaaatactaa aaatgaattc tgattcttac ttgattttag taggtgaagg gtcacttaga
    82261 aataaactga aagccaaagc agtagaattg gatatagaga aaagagtaaa atttttagga
    82321 gaaagaaacg atattcccga gttattatct gtgatggatg tttttgtaat gccttctctt
    82381 tatgaaggtc tgccaatggt tggattagaa gcacaagcag caaaattaaa attagtctta
    82441 gcagatacta tttccaaaga tacaaagtta acaaataatg tggattttgt atcattagat
    82501 gaaacaccac aagaatgggc aaaaaaagta ataaaagagc cgtatgctaa taaaataaca
    82561 caagaactaa agagttatga tgtttcgtac actactgaac aaatggagag tatatatagt
    82621 agagatcatt aaaaatgttc aagatatttt aggagtgatt atttttgaaa atatgttatt
    82681 tgatattggc atacaatgat attggcaact taaagagatt ggtcaatcga ttgaacgaaa
    82741 atgcagtttt tattattcat atagataaaa aaactgatat agaaccattt attgaagcat
    82801 ttaaatcata tgataatgtt gaatttttat gtaataaaaa ccgtgttaaa atttattggg
    82861 gtggtttttc cattgtacaa gc
//
LOCUS       DANJLF010000006        79058 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000006 DANJLF010000000
VERSION     DANJLF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..226
                     /locus_tag="PWT25_000473"
     CDS             <1..226
                     /locus_tag="PWT25_000473"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="broad specificity; family IV; in Corynebacterium
                     glutamicum this protein can use glutamate,
                     2-aminobutyrate, and aspartate as amino donors and
                     pyruvate as the acceptor; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL1020583.1"
                     /translation="FYIFPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPD
                     HFRIVYLPKMDDLKQTAKKMREFLATYRQK"
     gene            410..1246
                     /locus_tag="PWT25_000474"
     CDS             410..1246
                     /locus_tag="PWT25_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1020584.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            1322..2470
                     /gene="nagA"
                     /locus_tag="PWT25_000475"
     CDS             1322..2470
                     /gene="nagA"
                     /locus_tag="PWT25_000475"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL1020585.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            2690..3748
                     /locus_tag="PWT25_000476"
     CDS             2690..3748
                     /locus_tag="PWT25_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL1020586.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            3752..4786
                     /locus_tag="PWT25_000477"
     CDS             3752..4786
                     /locus_tag="PWT25_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL1020587.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(4888..5604)
                     /locus_tag="PWT25_000478"
     CDS             complement(4888..5604)
                     /locus_tag="PWT25_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1020588.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(5573..6358)
                     /locus_tag="PWT25_000479"
     CDS             complement(5573..6358)
                     /locus_tag="PWT25_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL1020589.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(6349..7098)
                     /locus_tag="PWT25_000480"
     CDS             complement(6349..7098)
                     /locus_tag="PWT25_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL1020590.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            7396..7920
                     /locus_tag="PWT25_000481"
     CDS             7396..7920
                     /locus_tag="PWT25_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL1020591.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            7936..9435
                     /locus_tag="PWT25_000482"
     CDS             7936..9435
                     /locus_tag="PWT25_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL1020592.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(9523..10125)
                     /locus_tag="PWT25_000483"
     CDS             complement(9523..10125)
                     /locus_tag="PWT25_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1020593.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            10229..11185
                     /locus_tag="PWT25_000484"
     CDS             10229..11185
                     /locus_tag="PWT25_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL1020594.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(11490..12446)
                     /locus_tag="PWT25_000485"
     CDS             complement(11490..12446)
                     /locus_tag="PWT25_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1020595.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            12620..13180
                     /locus_tag="PWT25_000486"
     CDS             12620..13180
                     /locus_tag="PWT25_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020596.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            13173..13997
                     /locus_tag="PWT25_000487"
     CDS             13173..13997
                     /locus_tag="PWT25_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL1020597.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            14007..14624
                     /locus_tag="PWT25_000488"
     CDS             14007..14624
                     /locus_tag="PWT25_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL1020598.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            14642..15325
                     /locus_tag="PWT25_000489"
     CDS             14642..15325
                     /locus_tag="PWT25_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL1020599.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            16008..17243
                     /locus_tag="PWT25_000490"
                     /pseudo
     CDS             16008..17243
                     /locus_tag="PWT25_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            17251..18138
                     /locus_tag="PWT25_000491"
     CDS             17251..18138
                     /locus_tag="PWT25_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1020600.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            18155..18991
                     /locus_tag="PWT25_000492"
     CDS             18155..18991
                     /locus_tag="PWT25_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1020601.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            18994..19617
                     /locus_tag="PWT25_000493"
     CDS             18994..19617
                     /locus_tag="PWT25_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020602.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            19614..20906
                     /locus_tag="PWT25_000494"
     CDS             19614..20906
                     /locus_tag="PWT25_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1020603.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      21265..21362
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            21478..22059
                     /locus_tag="PWT25_000495"
     CDS             21478..22059
                     /locus_tag="PWT25_000495"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL1020604.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            22062..23369
                     /locus_tag="PWT25_000496"
     CDS             22062..23369
                     /locus_tag="PWT25_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL1020605.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            23662..24150
                     /gene="purE"
                     /locus_tag="PWT25_000497"
     CDS             23662..24150
                     /gene="purE"
                     /locus_tag="PWT25_000497"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL1020606.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            24143..25267
                     /gene="purK"
                     /locus_tag="PWT25_000498"
     CDS             24143..25267
                     /gene="purK"
                     /locus_tag="PWT25_000498"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL1020607.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            25280..26575
                     /gene="purB"
                     /locus_tag="PWT25_000499"
     CDS             25280..26575
                     /gene="purB"
                     /locus_tag="PWT25_000499"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL1020608.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(26755..27132)
                     /locus_tag="PWT25_000500"
     CDS             complement(26755..27132)
                     /locus_tag="PWT25_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020609.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            27388..28116
                     /locus_tag="PWT25_000501"
     CDS             27388..28116
                     /locus_tag="PWT25_000501"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL1020610.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            28116..28370
                     /gene="purS"
                     /locus_tag="PWT25_000502"
     CDS             28116..28370
                     /gene="purS"
                     /locus_tag="PWT25_000502"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL1020611.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            28372..29043
                     /gene="purQ"
                     /locus_tag="PWT25_000503"
     CDS             28372..29043
                     /gene="purQ"
                     /locus_tag="PWT25_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL1020612.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            29044..31266
                     /gene="purL"
                     /locus_tag="PWT25_000504"
     CDS             29044..31266
                     /gene="purL"
                     /locus_tag="PWT25_000504"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL1020613.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            31251..32690
                     /locus_tag="PWT25_000505"
     CDS             31251..32690
                     /locus_tag="PWT25_000505"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL1020614.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32713..33765
                     /locus_tag="PWT25_000506"
     CDS             32713..33765
                     /locus_tag="PWT25_000506"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL1020615.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33762..34340
                     /gene="purN"
                     /locus_tag="PWT25_000507"
     CDS             33762..34340
                     /gene="purN"
                     /locus_tag="PWT25_000507"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL1020616.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            34337..35872
                     /gene="purH"
                     /locus_tag="PWT25_000508"
     CDS             34337..35872
                     /gene="purH"
                     /locus_tag="PWT25_000508"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL1020617.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35888..37162
                     /gene="purD"
                     /locus_tag="PWT25_000509"
     CDS             35888..37162
                     /gene="purD"
                     /locus_tag="PWT25_000509"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL1020618.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            37284..38198
                     /locus_tag="PWT25_000510"
     CDS             37284..38198
                     /locus_tag="PWT25_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1020619.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(38331..39896)
                     /locus_tag="PWT25_000511"
     CDS             complement(38331..39896)
                     /locus_tag="PWT25_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1020620.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39889..41340)
                     /locus_tag="PWT25_000512"
     CDS             complement(39889..41340)
                     /locus_tag="PWT25_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1020621.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(41354..42370)
                     /locus_tag="PWT25_000513"
     CDS             complement(41354..42370)
                     /locus_tag="PWT25_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1020622.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42590..43678
                     /locus_tag="PWT25_000514"
     CDS             42590..43678
                     /locus_tag="PWT25_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL1020623.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43732..45258
                     /locus_tag="PWT25_000515"
     CDS             43732..45258
                     /locus_tag="PWT25_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL1020624.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            45260..46465
                     /locus_tag="PWT25_000516"
     CDS             45260..46465
                     /locus_tag="PWT25_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1020625.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46722..47699
                     /gene="guaC"
                     /locus_tag="PWT25_000517"
     CDS             46722..47699
                     /gene="guaC"
                     /locus_tag="PWT25_000517"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL1020626.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47963..49387
                     /locus_tag="PWT25_000518"
     CDS             47963..49387
                     /locus_tag="PWT25_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL1020627.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            49848..50957
                     /gene="pdhA"
                     /locus_tag="PWT25_000519"
     CDS             49848..50957
                     /gene="pdhA"
                     /locus_tag="PWT25_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL1020628.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50960..51937
                     /locus_tag="PWT25_000520"
     CDS             50960..51937
                     /locus_tag="PWT25_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL1020629.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            51962..53605
                     /locus_tag="PWT25_000521"
     CDS             51962..53605
                     /locus_tag="PWT25_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL1020630.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            53613..55019
                     /gene="lpdA"
                     /locus_tag="PWT25_000522"
     CDS             53613..55019
                     /gene="lpdA"
                     /locus_tag="PWT25_000522"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL1020631.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            55181..55456
                     /locus_tag="PWT25_000523"
     CDS             55181..55456
                     /locus_tag="PWT25_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL1020632.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            55462..56229
                     /locus_tag="PWT25_000524"
     CDS             55462..56229
                     /locus_tag="PWT25_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL1020633.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            56397..58226
                     /gene="typA"
                     /locus_tag="PWT25_000525"
     CDS             56397..58226
                     /gene="typA"
                     /locus_tag="PWT25_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL1020634.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            58487..60259
                     /gene="recQ"
                     /locus_tag="PWT25_000526"
     CDS             58487..60259
                     /gene="recQ"
                     /locus_tag="PWT25_000526"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL1020635.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            60373..60675
                     /locus_tag="PWT25_000527"
     CDS             60373..60675
                     /locus_tag="PWT25_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL1020636.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            60678..61841
                     /locus_tag="PWT25_000528"
     CDS             60678..61841
                     /locus_tag="PWT25_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL1020637.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            61867..65295
                     /locus_tag="PWT25_000529"
     CDS             61867..65295
                     /locus_tag="PWT25_000529"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL1020638.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            65316..66440
                     /locus_tag="PWT25_000530"
     CDS             65316..66440
                     /locus_tag="PWT25_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020639.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            66430..66867
                     /locus_tag="PWT25_000531"
     CDS             66430..66867
                     /locus_tag="PWT25_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL1020640.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            66851..67189
                     /locus_tag="PWT25_000532"
     CDS             66851..67189
                     /locus_tag="PWT25_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL1020641.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            67308..67868
                     /gene="rsmD"
                     /locus_tag="PWT25_000533"
     CDS             67308..67868
                     /gene="rsmD"
                     /locus_tag="PWT25_000533"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL1020642.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            67865..68356
                     /gene="coaD"
                     /locus_tag="PWT25_000534"
     CDS             67865..68356
                     /gene="coaD"
                     /locus_tag="PWT25_000534"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL1020643.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            68346..69389
                     /locus_tag="PWT25_000535"
     CDS             68346..69389
                     /locus_tag="PWT25_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL1020644.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            69548..70276
                     /locus_tag="PWT25_000536"
     CDS             69548..70276
                     /locus_tag="PWT25_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL1020645.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            70392..70637
                     /locus_tag="PWT25_000537"
     CDS             70392..70637
                     /locus_tag="PWT25_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL1020646.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            70634..70852
                     /locus_tag="PWT25_000538"
     CDS             70634..70852
                     /locus_tag="PWT25_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL1020647.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            70988..71527
                     /locus_tag="PWT25_000539"
     CDS             70988..71527
                     /locus_tag="PWT25_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL1020648.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            71520..73793
                     /locus_tag="PWT25_000540"
     CDS             71520..73793
                     /locus_tag="PWT25_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL1020649.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            73831..74877
                     /gene="holA"
                     /locus_tag="PWT25_000541"
     CDS             73831..74877
                     /gene="holA"
                     /locus_tag="PWT25_000541"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL1020650.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(74966..75220)
                     /locus_tag="PWT25_000542"
     CDS             complement(74966..75220)
                     /locus_tag="PWT25_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL1020651.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(75620..75889)
                     /locus_tag="PWT25_000543"
     CDS             complement(75620..75889)
                     /locus_tag="PWT25_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020652.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(76359..76814)
                     /locus_tag="PWT25_000544"
     CDS             complement(76359..76814)
                     /locus_tag="PWT25_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL1020653.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(76952..78880)
                     /locus_tag="PWT25_000545"
     CDS             complement(76952..78880)
                     /locus_tag="PWT25_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL1020654.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        25677 a        13614 c        17128 g        22639 t
ORIGIN      
        1 tttttatata ttcccaaaaa ttgatacaaa gcgattcaat attttggatg atgaaaaatt
       61 cgttttggac tttttacatg aacatcatat tctacttgta catggtggtg gatttaattg
      121 gaaccagcca gatcattttc gaatcgttta tcttcctaaa atggatgatt tgaaacaaac
      181 agctaagaaa atgagagaat tcctagcaac ctatcgccaa aaatgatttt aaaaaccaaa
      241 tttttcagta ggagggccac agacaaacac actgaggctc tctttctagt ggagttttct
      301 taaataacta aagtatatta tttagttgca agtcttttct tgaagtttgc gacttcctaa
      361 agtaaaatac gaatagaatg cgaatacgca tattttgggg aggatactta tggaaacgtt
      421 aatcgagtcc tttggaacga tcattaaaga gattcgaaaa gaacaaaaaa tgacgcaaaa
      481 aatgctttcg catgatattt gttcacaaag tgtattaagt cggatcgaaa ataatgagga
      541 actgccgaat gtattggtga tgatgcagat ttgtcaacgg ctaggggtaa cgattgatca
      601 tgtgatgaat ctctctcgaa aaactactcg gacaaacgat aaattatttg aactgttgga
      661 tagttgtttc caaaaacgtg attatcggaa gttagaacaa gtactaaaat cttctgatat
      721 ttctgaaaat ctgtatcaag caactgactt tcaacgctat tattattatc tcggtgtttg
      781 cgaattcacg ttgcgtcaaa atatctctca gtccctttat tatttgaaag aagcattaag
      841 ctactctagc caaaaagaaa gaatccatgt ttctgacaca gaaatacaac tgattagttg
      901 tatcggaaaa atatatggtg tggcaggaaa aacggctgaa gcacgctact atttgagtcg
      961 aagcatccaa ctgctgcatc aatcagctga tgaacgatca aaaagtgaat tttcgcaaat
     1021 tttttataat tatggaaatt tcttattcca tcaaaatgaa atcgacgaag cgttagaaca
     1081 ggtgaatcaa ggaatctatt gggctcaaga gaagaatagc tactattacc tcaatgattt
     1141 atttgtgctt aaaagcttaa tatacaagcg taaagaagaa ccagaaagag cagcattcta
     1201 tgaaggctta gcacaagctg tgaaacaaat ttcaaaaaat ttgtaaatgg tgtagaccat
     1261 tcttgacttc ttctctaaat aagagtactt tagaggtaga aaaacaaagg gagagaatag
     1321 aatgagaact tttatctatg ctgacaaatt ctttttagcg tcaggtgtca aacatgcagg
     1381 ctatcttgaa attaccgatg gactttttgg tgaatacacc aaagaactac ctgagggaga
     1441 agtcaacatc attgaccaaa gcggcaaatg gattgctcct ggtttggtag atacacatat
     1501 tcacggctat atgaatcatg atgtaatgga taatgatgca gaaggcatca aagtgatgtc
     1561 tgaaggtctg ttatcttgtg gagtgacttc atttttacca acaacattga cgtcaagcaa
     1621 agaacgttta aaagatgtag ctgagacaat cggcaaaatg tatcaggaag caccaggagc
     1681 taaaatcaaa ggaatctatt ttgaaggtcc tttcttcaca gaagaacaca aaggggcaca
     1741 aaacccaagt tatttcgggg atcctgattt ggatactttc catgaatggc aagaagcttc
     1801 aggtggttta atcaaaaaaa ttgctttagc accggaacgt caaggtgtag aagagtttgt
     1861 caaacaagtg actgaagaag gagtagttgt ttctctagga cacagtaatg ctacactaga
     1921 agaagcgcaa aaagctgttg aagcaggcgc aagtgtattt gtccatgctt tcaatggtat
     1981 gcgtggattg aatcatcgcg aaccaggtat ggtgggtgcg ttattatctt tagaacatgt
     2041 cttttctgaa ttgatttgtg atggccatca tgtacatcca aatgctgcgg gaatcctaat
     2101 ggaaaaagca ggacatgatc atgtcgcatt aattacagac tgtatgatgg ccggtgggat
     2161 gcctgatgga aattacaatt taggcgaatt tccagtagta gtaaaagaag gaacagcacg
     2221 tctagaatct ggcaacttag ctggaagtat attgaaattg aaagaagcaa ttaaaaatgt
     2281 tgtagactgg ggattagcaa caccagaaca agcagtcatg atggctagtc tgattccagc
     2341 aatcagctgt aaaatcgatg atacatgtgg aaaaatcgct caaggacgcg atgcagactt
     2401 tatcgtattg aaccctgata tgacattgga agcaacttat ctagatggcg tggaacgttt
     2461 ccacgcataa tataatttaa gttaacctta aaactaaact tcttaagttt ggttttaagg
     2521 tttttttgtg ttctagtaaa aatcacttta cataagtttc aaaagtttct ttaatttcct
     2581 attgttcaat aagtaacaat gtaaagataa aattaaaaat gttaatacgt attagcaaaa
     2641 taaaagcttt ttttattgtg aataaatgaa cataattagg aggagaaaga tgaacttatt
     2701 tgacattttt cgatcgactt tgacagatat gtcgattgtc agtgcaatca cttcaacagt
     2761 ttttattata ttattagggt ttgtttgtag aaaaaagggg attttttctg aatctgttgg
     2821 aaaaattcta tcgaaagtcg tcttaacagt ggctttgcca gcactggcat ttcgagcttt
     2881 catgcaagat atcaatacag aattactgat gcaaggaatc aatgtcttga tttggggatt
     2941 agttatttat attattctta ttctagtttc aaaacctttt ttcttaagct ataaagggga
     3001 taagcaaaca acgttgcgtg tcctgacgat ctttggttct acgacctttt tcggaacgcc
     3061 tatcgtcagt gcgatttatg gtcctgtcgg cgtgatgtat tcctcaattt tcaatattgg
     3121 ctaccgaatc tttttgtatt cttatggata cataaaaatg agtggcttga agatggaagc
     3181 taaaaatatc aaagcgatgt tcgtcaatcc aatcattatc gctacttttg ccggcctttt
     3241 catttggctg ttccaagggt atctgccaca agtgtcagtt acaggtgcag aaggagagat
     3301 aagccaagta gcgtttttgc gaattgacca gactgcaccg tggctatttc agccgatgga
     3361 ttatttagct ggtttaagtt caccattggc gtggctagca atcggcgcaa cattaggtga
     3421 agtaagcttc aaaaaagctg catctgaaaa aacttcatgg tattacagtc tagtcaaagt
     3481 ggtatttgta cctgcattga atatcgtgat tctagcagtt ttgacggcaa cgaatattct
     3541 accagtcaat tttgaagcac tggcaacaat cgttatcatg atggctacac cagctgcaac
     3601 agtggcagca gcctatgcta tcagttttga tagggaggca cttttagcct ccaatgcatc
     3661 cttactgtca actatttttg ctgtggtact gacacctgta tggatcgttg tattacaaat
     3721 gattcatggc ataggtttat tttcataaga aatggaggaa gaaagtatga gaatcgcatg
     3781 ttacggtgtt cgtccgaacg agatagattt ttttgaaaaa ttgaatattt accattatga
     3841 tctcagtctt tatgaagaac tgttgacaca tgacaatatc gtgtcagcaa aggaccatga
     3901 tgctgtattg ttgaggggaa attgtatagc agatgagacc aatcttgcaa aaatgcagga
     3961 atatgggatt cgttatgttt ttacacggac tgtgggagtc aatcatatcg atctacaagc
     4021 ggcagctgat tttggtatga cagtagcccg tgtaccatcg tattcgccaa atgcgatagc
     4081 agagttatct ttgacttttg ccatgatgct tttgagaaat actgcgtata cgacgatccg
     4141 aacatctttc aaagattttc gagtggatga gcaaatgttt agtcgtgaaa tcagaaattg
     4201 cacagtaggg atcataggaa caggcaggat agggttgacg gaagctgttt tgtttaaggg
     4261 actaggagcc aaagtgatcg gctacgatat tcatcagaca gaagctgcta aaaaagtatt
     4321 gacgttccac tcgcttgaag agttattagc caaaagcgat attgtcagta ttcatgtacc
     4381 tcatatccca ggtgaaaacg atcaaatgat caatgaagga tttttagctc aaatgaaaaa
     4441 aggtagcatc ttgattaata cagcccgtgg agagcttcaa gataatgaag ctattttaag
     4501 agctttgaaa agcaatcatt tagctggttt tgcaaccgat gtttttgcaa atgaaacaga
     4561 ggtttttttc cgatccttca agccatggga gacgattcca gaccctgcta tccagcaatt
     4621 agtagaaatg taccctagag ttcttgtaac cccgcatgta ggttcaaata cagatgaggc
     4681 attatcaaat atgatttcga caagtttcga gaactttcgt gaaataatag aaactggaaa
     4741 aacgaaaaat gaggtatctc ttcctaaagc ccgtcaatta agataagaaa acatggatat
     4801 tcgtcaataa aatgtttaaa aatagtccaa gtaaacagtt gttacttctg tatacttgga
     4861 ctattttgta tgggataatt cgatgactca acagtaatct gtgagctttt tccgcccatt
     4921 ctttgtcaat tcataacagg ttagcggctg gccgatttcc aaatatttga ttctttcgtt
     4981 caatatatct tgttctacta aaaaattcaa atatttcttg acggatatac gtgacagtga
     5041 gcttttttga gccagttctt ttgttgaaaa aatagtatct tcttttagaa taaatgcact
     5101 tatttttttt aaagtcagtt ttgacagacc tttaggcaaa tcgatgaatg atggtatttc
     5161 gtgaatggga gtggtgtggt tgaatacttg atcgatcaat ttctgatcgg ctttttcagt
     5221 cgtttggatt gtctgagaga aagcaagata cttttcgatc gccagctgaa aacgttgcaa
     5281 agtgaacggt ttgatcaaat aatctaaggc accgtagcgg atcacattct ttatggtttc
     5341 gatatcatct gcagcagtga tcaaaatcac tgggatatca tttccttgct gtctaagcca
     5401 agataaaaaa tggataccac tttcgccagg catgtataca tcaagcagca gcagatctgc
     5461 gtttttttgt ctgaaatatt tttttccttc ttctacattt ctgaaacttt cgattacgtt
     5521 catattaggg atgctttcca caaaagtacg attcaattct gcaaccattg gatcatcttc
     5581 gattatcagg acatttgtca tcattttgat cttccttttt tggtaattct agatagacga
     5641 ttgtaccgcc atttgtttgt ttggaaaatt ctaaaaatcc accgtggcgt ttgacgattg
     5701 aatggatgag attcaatcca aacccacgat tgattccctt cgtcgaaaaa cctagcgtaa
     5761 atattttttc tgagatttct tctgatatcc cgacaccgcc atcgcagacg gtcacgatga
     5821 atacttcagc ttctgtgtca tagtttaaat gaatccggac ctgtccgttt ctttcatgtt
     5881 tttgtagcac ggcttctttt gcattatcaa gtaagttccc taacacttgc aaaagtgaat
     5941 ggatggagtc atccatctcc attttaggga taaaagaatc gctttctaac agcatttcaa
     6001 cagattgttc tttggcttcg tttattttgc ctaataaaaa accggcaatt gcaggtactt
     6061 tgatcagact tgagacttgg ccaatctctt ttttataatt ttgatttagc tgctggataa
     6121 aatgggtgac ttcttcatac ttcttcaaat caatcaaacc agaaatgaca tgaagtttat
     6181 tcatgaattc atgggtttga gctctcatgg agtttatata ttgtttcgtc cccccaagct
     6241 cctccattaa tttcattact tctgatcgat ttcgtaaagt tgcgactgct cctaaaaaac
     6301 ggtcattgct aaaaagaggt gtaacgctag aaatagtttc tatcccattt aaatacatgg
     6361 attgagtccg aacggtcgtt tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat
     6421 tatcactgat ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt
     6481 ttttgcaggt gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc
     6541 atctacttca ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc
     6601 aagtccaagc agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac
     6661 gctaagtcca ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc
     6721 aatcgtgttg tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat
     6781 agggacgaaa tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg
     6841 tcctccaatc acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg
     6901 gtctgcgtga gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga
     6961 tgcaatcatg atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag
     7021 ctttttttgg atgagtttat cattggcaac gattttagca atgctagaga ttttatctga
     7081 cacactagat cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa
     7141 agaaacgaat gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt
     7201 tttcttaaaa aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat
     7261 gaaaacaaaa atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac
     7321 tgagtcggta aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg
     7381 gaaggaggcg tgtatatgat agggggaatc gcacttgaga cgatttattg gtatacattg
     7441 ctgatttgtg caggattggc agtcctattg attatttttg gagatgtatt cgattttgat
     7501 ggcccgcttg atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt
     7561 ctaggtgagc gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata
     7621 tcgatagtgt tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa
     7681 tcaacgatgt cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg
     7741 atccctgtac gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt
     7801 ccagcttgtt tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc
     7861 attgagatca aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa
     7921 aggagcgtgt gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg
     7981 attttactga tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa
     8041 gcgttgatca tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat
     8101 aacaaaatca aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg
     8161 aatcggatca gtctattatc gagtaaattg gacgtctcca cccctgaagt gtataccgaa
     8221 caaggcgtac cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa
     8281 gagattgcta cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa
     8341 gcaagagaag tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa
     8401 atctatcaaa atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt
     8461 gcaaaaatgg ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt
     8521 tatcttgact ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca
     8581 gaagcggaag ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa
     8641 gcagctgaat tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa
     8701 ttagcaacct acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat
     8761 ttagaaagtg cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta
     8821 gaacgccaaa aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat
     8881 gattcggaag tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa
     8941 gctcaaaaag tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg
     9001 gcagaagcgg aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta
     9061 gcaaaaggaa aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat
     9121 ggcgaagcag ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca
     9181 gcacagccgc taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga
     9241 gaaaccagtg gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa
     9301 gaaacactaa aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca
     9361 cgtgggacta ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg
     9421 atgaaggaag aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt
     9481 aaagttcttc tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt
     9541 cttttgtttt tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt
     9601 cttttggagt tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg
     9661 aagaaagaaa ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg
     9721 gatagcatac aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc
     9781 tattgcggaa atgataccgg aattattgtt aataccatct gacacgacga tcacaataat
     9841 aactagaata ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac
     9901 aaaaaaataa atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc
     9961 aaaagaaacg gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat
    10021 catcgggatt cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc
    10081 acttttgatc gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt
    10141 tggtcttttt actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt
    10201 ataattaaaa taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt
    10261 gatcagtgtc ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc
    10321 tgcagggatc attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg
    10381 catgaatcga atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc
    10441 tgttgggtat actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc
    10501 tcgcttcaaa ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt
    10561 ggaggaaaac atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt
    10621 agtatttgct ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa
    10681 ccaaatacag atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt
    10741 atcagaaaat aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga
    10801 aagccaagaa gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta
    10861 cgaaacttac ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa
    10921 tctagagaga atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta
    10981 tactgcagaa acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga
    11041 agtttatgta ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg
    11101 agcagaattg ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt
    11161 ctcccaacaa aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag
    11221 ctgatttcac aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca
    11281 caatattcaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga
    11341 acggtaggct cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa
    11401 gtccagccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    11461 cagaacagct ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga
    11521 tccaagattt cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca
    11581 atcatgtgta ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg
    11641 atgaattcct ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca
    11701 gcaatcgtca cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga
    11761 aaagtcaagc tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt
    11821 tcgtccacgc ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc
    11881 aaaaagaaac gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct
    11941 aattctggat tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct
    12001 tggatcattt ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa
    12061 agtggcatgt tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag
    12121 cgcatagcgt cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt
    12181 ttgcctgctt ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg
    12241 tacacgatgt cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga
    12301 atattgtaac ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt
    12361 agttcgctcg tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca
    12421 acgattcccc attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc
    12481 ttgtctgtct attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat
    12541 taaaaaaaat gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag
    12601 ttaaaaggag gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt
    12661 agaacagttt gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca
    12721 atggcaaaaa tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa
    12781 aaatcttttc actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat
    12841 ttttcccgaa aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc
    12901 gcaaaaatgg cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca
    12961 gcaggctggt agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga
    13021 tgatattttg aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat
    13081 cgaacttctc gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg
    13141 ggaacgtgaa gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag
    13201 ttgtgattat gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc
    13261 aagccggcaa tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga
    13321 acagtcaaac aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac
    13381 aattgccaga tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat
    13441 tagaaaaaga aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg
    13501 acaaacgcat tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc
    13561 cattagtgat ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa
    13621 atgtccaaga tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta
    13681 aagaaacgat tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg
    13741 tcgatcaaaa ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc
    13801 agcctggagc taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc
    13861 caaaaggaca cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg
    13921 gtaaagaaaa cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg
    13981 aattaaatga aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca
    14041 agaatatccg ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt
    14101 tttccttgaa gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat
    14161 cccatcagca atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg
    14221 gagtgcttcc tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta
    14281 ccttggctag caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag
    14341 taagcctgga aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca
    14401 aagtgtatga ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag
    14461 tagattggaa tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg
    14521 cacaagcatt gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc
    14581 aggtcatcga gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct
    14641 gatggcagaa caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt
    14701 accggaaatc attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat
    14761 catctttact acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt
    14821 gtatcctttt acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc
    14881 agttttctcc ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat
    14941 gttcgcagaa gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac
    15001 agtaaaagaa gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata
    15061 tacagaaata gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa
    15121 agaaactgca gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc
    15181 gacaggtggt ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat
    15241 cacatatacg ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga
    15301 aactgagaaa aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag
    15361 tggctcttcc tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa
    15421 atttttttgt ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt
    15481 agcattctaa caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga
    15541 ataaaaacta aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa
    15601 gtcagcggga actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat
    15661 tgtacctaat ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca
    15721 cgaagaagca tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt
    15781 ttctttgcgt aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag
    15841 aatcagcact atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata
    15901 aggcaagtct tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata
    15961 aaaatacagt atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa
    16021 tgagaatcat cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag
    16081 atgacaaaaa aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa
    16141 cagtaaaagt aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat
    16201 tttatgatta tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct
    16261 gggacatttt ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag
    16321 atgaatatgt ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa
    16381 cgacgactta cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg
    16441 cttacaataa aaatatattt gaacaattaa atatccctga accagcttca aattggacat
    16501 gggatgaatt tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt
    16561 caagattcga agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat
    16621 tgaattttta taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc
    16681 taacagattt tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag
    16741 gtggtggagc tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg
    16801 caatggtgtt tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata
    16861 tggagattca gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt
    16921 tacgttcttc gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta
    16981 agtttattga tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg
    17041 gtgtaccaat catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc
    17101 aagcgacatt tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct
    17161 ttgatagtgc tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca
    17221 tttatgaaga agtaacagca gaaggaaggc atgaaaaaag aaaaactaaa acggttcttg
    17281 gatcaagaca atgtggtcgg ttacgtattt gcttctccat tcatcctagg atttctattg
    17341 ataacagtga taccgatgat cttgtcactt tattattcgt tcaccgatta tcgtttggga
    17401 aatccagtct catggatcgg cttggataat tatataaaga tgctgagtga tgcccgtttt
    17461 ttgaactcca tccgagtcac ttttctctat gtatttgcat ctgtacctgt gaaactgatc
    17521 tttggtctat tgattgctta tcttttaacg aggaatatca aaggaacgac tttctttcgt
    17581 tctttgtatt atgtgcctag tttgatcggt ggcagtattt ctatcgctct agtttggcgg
    17641 gagttgtttt ctaaaaacgg gttagtgaat attttcctgg aacgattagg cgcacctgcg
    17701 ctttcctggt ttagcgatca aaaactggtc atgatccctc ttgttttgat gtctgcttgg
    17761 caattcggtt cggcaatgat catctttgct gcaggtatca aagagatccc aggctcgtat
    17821 tatgaagcgg cagagatcga tggagcgaac aaatggcaga cgttttacaa gatcacgtta
    17881 cctcttcttt cgccgatcat tttgtataac ttggttatgc agacaatcgc ggcgtttatg
    17941 acttttacgc aggcatttat cattacccaa ggtggtccga atgatgccac gaacttttat
    18001 gctctttatg tgtataacca agctttttct tttaattcta tggggtatgc tagtgctatt
    18061 tcgtggatca tgctgttgat tatgggagct gtcacctttc tgattttcaa atcatcaaaa
    18121 aaatgggtcg ttgtgtgaat gagaggagga agaaatgaat aatacagtaa cgaaatggtt
    18181 tcaatacggt tttattttgc tattaggtct agtcatgatt tatccggtct tatggatgat
    18241 cgcaggttct tttaaaacgg atcaagagat tatgagtggt tcgctgaatt tgattccaga
    18301 gacttttcaa tgggaaaatt acgcaaaagg atgggcgggg tttgctggaa tttcattttt
    18361 tactttcttt aggaattcgt ttgtcatctc tgcagtttcg acgataggga cagttctgag
    18421 ctcgacttgt attgcttacg cattatcgag aatcgatttc aaaggaaaaa gattctggtt
    18481 cgtcgtcatg ttagtaacga tgatgattcc tgcacaagtg attttgattc cgcagtttat
    18541 tatttacaat cggctgaatt tggttgggac gtatgtgccg ctgattttac cacatttttt
    18601 tggacaagca tttttcattt accagatcat gcaattcatg gtcagcatcc ccaaagagtt
    18661 agatgaatca gcgattattg acggatgcag caagtatagt gtctttacca aaattatttt
    18721 tcctttgctg aaaccttcga tcatcacgac gatcatcatc cagttttatt ggaaatggga
    18781 cgacttcatg ggaccattga tttatttaaa caagccgaga tcttacacgg tatcgattgc
    18841 catcaaatta tttgcagacg ctggatctag tacgacttac gcgagtatgt ttgcgatgtc
    18901 tacgttgtcg ctgctaccgg tttttctgat tttcttattc ttcaataaat atcttgtcca
    18961 ggggatcagt acaagcggtc tgaagggctg aatatgaaaa agccaataga agagatgttt
    19021 ctttacaagc tcaactacta tgtctgtcgg atttttttaa ccagtcttat cagtaatgct
    19081 cttttcgctt tagttctagg atgtttgttc actttaggat tagtgctgtc tgctcgtgtt
    19141 gtctatctga tttatttttt gatgatgtat ccttcttatt atctatctgt ccattacttg
    19201 gcagaaaaaa aggtatgttg gaggaaaggg aaaatcaaac ggatgtgttt catcattacg
    19261 ttgtttcagc tgtttgtcat tatttttgca gttgattggt atttattagt gggaaagatt
    19321 ggagcttggt atctgattcc ttttctattg tgtctgttgc tttttgtatc ccatagtttt
    19381 tttagtacgc taattgatat ctgcaaagaa gataaagcag atagtcttgt ttatttgatc
    19441 aaaaggaata tctggctgtt gtcaaaaaac gtcaaggggt ttctgtggta tttatgcctt
    19501 ttgtatggac tgttctggtg tatacagcct gattttttac tcactgcatt actgtatcca
    19561 gggctgccat ttgtcacagt attttgctta aaagaaaagg agaatgactt taaatgaaga
    19621 aaaaatatgt acaagtcgga cttggtggga gagcgcgttt tttttatgag gcaattgcct
    19681 ctacgtatca tcaaacttgt gaactagtcg gtttttgtga tgtatctcaa acaaggatgg
    19741 attatgcaaa tcggatactt gtgaacaagt atggacagaa aaaagtaaag acatgtatgc
    19801 catcacaatt tgagcaacta ctggatgacg tacggccggc tgaagtcatt attacgtcag
    19861 tagatcgaac gcatcatgat tatatcattc gtgcaatgaa aaaaggatat gatgttattt
    19921 gcgaaaaacc tctgactatc gatgaggaaa aagctcaaga aataataacg gtccaaaaac
    19981 agacgggaag aagcctgcga gtcactttta attatcgtta tgcaccgtat cacacgaaga
    20041 taaaggaact gatagaaagt gatgtgattg gtgatgtgtt ttctatccac ttcgaatggc
    20101 tgttaaatac gcaacacggg gcagattatt tcagaagatg gcatcgaaat aagcacaata
    20161 gcggcgggct tcttgttcat aaatctaccc atcattttga tctagtgaat ttttggttga
    20221 acagccgtcc ccaaaaggta tttgcattcg gtggattaaa cttttatggg atgcataatg
    20281 cggagaaacg cggagtagaa atattttaca gtcgatgcta tggaagcgaa ctagctaaag
    20341 ctgatccatt tgctatagac ctgtcagcca atgaggaatt gcgagaaatg tatttagaag
    20401 cagaaaaaga cagtggatat attcgtgacc gcagtgtatt ttccgatgat atctctattg
    20461 aagatacact aggcgtaacg gtcaaatatg aaactggtgc gattttgacg tattcgctaa
    20521 acgcgtatat gccgtgggaa ggattcaatg tttctttcaa tggcagcaaa ggacggatcg
    20581 aaatgaaagt gatcgaaaag tcttacatca atgcaggtgg agataaaaag gatgaaggag
    20641 cagccgttct tcagtccatc catgtctatc cgatgttcgg tgagccatat gaggtaatcg
    20701 tcgaacaaga agcgggcgga catggcggag gagatccgcg tttattagat gatctatttg
    20761 gtgaagaaaa agaagaagat ccgtggaatc gggcagcaac tcacgtagat ggtattttgt
    20821 cgattttgac aggaatcgca gcaaatcatt ctattgcttc aggaaaagca gtagtgattg
    20881 acgagcttgt cagcttaaaa gaataagaat agatttacgg aaattacttc ccgtagttta
    20941 taaaatcttg ccttattgcc gataggctgt cttttgaaca ctatttattc ggaaaaaggg
    21001 gctatgacat gctttttgtc atgccttctt tttatgtggg ctgctttttt agataggaga
    21061 aaataaaaat aactatttaa acccgaacaa aaaggaataa tgcattaaaa atcattagat
    21121 tttacaaaaa aataagtaaa ccacgttgtc agtttcagaa agtagtgtta gactgttttc
    21181 aatatttgaa acaacatttt ttattcaaat atggcaaact gccttcacgt gcaaaacagt
    21241 tgcaagaaaa tgaatttaca ctattattag ttttacatac ctacttatat atcgtcataa
    21301 tatggatgac agtttctagc cagtaccgta aatgctggac tataagtaaa agattggcta
    21361 tttcaccctt cttttcttat gataaagtgg agggtgtttt tctttaacag aaacatagta
    21421 ggtttttgca caaaagcggt tatttcggta ttaataaatg ataaaggagc aaataaagtg
    21481 aaagaattag ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg cgtattaaaa
    21541 gtcgacagct tcgtcactca ccaagtagac cctgtattga tggaacaaat gggaaaacgg
    21601 tttgcagaag tatttaaaga acaaggaatc acgaaagttg tgaccattga agcttctggg
    21661 atcgcaccag ctttatttgc tgcaagagaa ttagatgttc caatgatctt cgctagaaaa
    21721 gcaaaaagtt tgacgatgga tgaagaatta ttgacagctt ctgtttattc ttttacgaaa
    21781 caagtgacaa gtacgatttc tatttctcgt aaatttttat cttcagaaga caaagtattg
    21841 attatcgacg atttcttggc aaacggacaa gcggcaaaag gattgatcga attatgccag
    21901 caagctggag cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt ccaagatgga
    21961 cgtcagttac ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat tgcttccttg
    22021 acgaatggac aagttgagtt tcttgaggag gacgcataat cgtggaaaaa gaaacacaaa
    22081 atggaaaagc tgcggtttta gggctgcagc atctgctagc gatgtatgca ggagcagtag
    22141 cggtgcctct tttgatcggt acaggactag gctttaatca agaacagatg acttatttga
    22201 tttcaattga tatctttatg tgtggattag cgacattatt gcaattgaca gttaaccgat
    22261 ttttcggaat tggcttgcct gtcgttttag gatgtgcgat ccaagcagta gctccactga
    22321 tcttgatcgg aaccaatgaa ggagtaggag ctatctacgg atctattatc gcttctggga
    22381 tctttgttgt gttagtttct ggtgtctttt ctaaaattaa aaaactattc cctcctatag
    22441 taacaggaac ggttatcacc gttattggtt tgactttgat tcctgtagca atcgagaaaa
    22501 tgggtggagg taatgcaaca gcagctgatt ttggtgacaa aacaaatctg ctacttgcat
    22561 ttgtgacgat tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc attcgttcaa
    22621 tcgcggtatt gattggttta gtcggaggaa gcatcttagc tgctttcatg ggaatgattg
    22681 atctgagcgc gattggagat gcgccgttgt tccatgttcc acaacccttt tattttggta
    22741 aaccaacttt cgacgtatgg tcgattttac taatgatcat catttccatg gtcagcatgg
    22801 tagaatcaac aggtgtatat tttgcacttg gtgacattac tgggaaaaat gttggagaag
    22861 aagaattgaa aaagggatat cgtgcagaag gacttgccgt tatcttaggc ggtattttca
    22921 atacattccc ttatactggc ttttctcaaa atgtcggatt ggttcaactt tcgggaatca
    22981 aaacaagaaa acccatctat ttctctgcat tcttcttgat tgttttagga ttattcccta
    23041 aaatcggtgc gttagcacaa atcatccctg agccggtact tggtggagga atgttagtca
    23101 tgttcgggat ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat ttcagcaatg
    23161 ataaaaatct attgattgtt gctgtttctg ttggatttgg gctgggtttc aatgttacgc
    23221 cgacattatt cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat ggcatcgtaa
    23281 tgagtagcat tacggctatc atattgaatt tgatctttaa tggattgaaa aaagatgaag
    23341 aaatattgga agaagccttc aagaagtaat agagcaaaag aaaggttgtg aaatcagcgt
    23401 tttgacctga ttgttagaga aacaaacgga aaaatagttc ctcgtatttt gccgtttgtt
    23461 tgagctaata ccgagggtca gctgattgaa caccgtttat ccataaaaag gaatgtgacc
    23521 taacttttgt cacattcctt tttttcatct ttaataaaca cgaacaataa aatgtttttc
    23581 tttgttaaca ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta
    23641 gaaaaactgg gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca
    23701 gattgggaaa caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa
    23761 aaaaaggtcg tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct
    23821 cgaaaaaaag gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt
    23881 atggtcgcat caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta
    23941 aatggtttgg attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact
    24001 acagcaatcg gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc
    24061 atgtatgatt tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact
    24121 gttatagaaa gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat
    24181 cgtcggtggc ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa
    24241 agtaggtgtg ctggatcctg tgagcgattg tccagccgca caagttgcag attggcacat
    24301 tgaaagtgtc tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat
    24361 tacctatgaa ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt
    24421 tattcctcaa gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt
    24481 tttggaaacg aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat
    24541 ccaagatgcg attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta
    24601 tgatggcaag gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct
    24661 tttaagagaa ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat
    24721 catggtggca ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca
    24781 ccacaacaat attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga
    24841 ggaggctgag cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg
    24901 ggtagaaatg tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc
    24961 tcacaattct ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat
    25021 tcgaggtatt tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat
    25081 ggtcaatgtt ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga
    25141 ctggcaattc catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat
    25201 cactatcttg acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg
    25261 ggattagagg agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg
    25321 gacggaagag aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg
    25381 ggcagaactt ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt
    25441 tgatatcgat cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac
    25501 acgagcggtt tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag
    25561 tacggatgtc gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg
    25621 ggaagacttg aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac
    25681 agtgatgatg ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact
    25741 tgccacatgg tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg
    25801 agtagaagca ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat
    25861 tgaagagtat gtatgtaaac aattggggat ccgtccacaa gaaatctcta ctcaagttct
    25921 ccctcgggat ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga
    25981 acgttttgca acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt
    26041 ttttgcgaaa ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc
    26101 agaaaatatg gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa
    26161 tgtcacatta tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc
    26221 tgataccacg attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct
    26281 aacggtcttt cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag
    26341 ccagcgcgtt atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct
    26401 tgttcagcca aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga
    26461 agaagatcca accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta
    26521 tcatcatcat ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag
    26581 agtgagttgc aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc
    26641 cgttagcggt tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat
    26701 tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc
    26761 gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc
    26821 gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt
    26881 gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata
    26941 aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa
    27001 tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt
    27061 atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat
    27121 tgcatcttcc atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta
    27181 atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg
    27241 ataaaacacg aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat
    27301 aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt
    27361 taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa
    27421 gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa
    27481 gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat
    27541 caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt
    27601 gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg
    27661 gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa
    27721 gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt
    27781 atcaatgaag agcatatcta tttcttacaa cttgcaaaac cggaagaaat cagtgagctg
    27841 aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg
    27901 gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat
    27961 gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa
    28021 gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt
    28081 ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa
    28141 cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta
    28201 tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg
    28261 atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg
    28321 taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt
    28381 gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag
    28441 atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat
    28501 ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct
    28561 cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc
    28621 ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga
    28681 cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg
    28741 gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa
    28801 ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc
    28861 tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa
    28921 gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga
    28981 tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta
    29041 tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc
    29101 gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg
    29161 cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa
    29221 aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct
    29281 ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa
    29341 agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga
    29401 atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg
    29461 ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc
    29521 ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct
    29581 tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat
    29641 atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca
    29701 ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa
    29761 cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg
    29821 tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca
    29881 gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg
    29941 acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg
    30001 gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa
    30061 ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa
    30121 tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa
    30181 gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag
    30241 ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag
    30301 caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc
    30361 aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa
    30421 gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga
    30481 ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg
    30541 gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta
    30601 tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca
    30661 ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt
    30721 ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca
    30781 ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa
    30841 aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag
    30901 agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac
    30961 tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg
    31021 atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt
    31081 gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct
    31141 gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag
    31201 atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg
    31261 aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg
    31321 ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta
    31381 tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg
    31441 tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac
    31501 gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc
    31561 atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa
    31621 aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc
    31681 atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg
    31741 aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg
    31801 acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg
    31861 cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg
    31921 gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc
    31981 agctttctat ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag
    32041 ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag
    32101 taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg
    32161 cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa
    32221 catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg
    32281 tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta
    32341 cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga
    32401 ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta
    32461 aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt
    32521 ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc
    32581 cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact
    32641 atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca
    32701 agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa
    32761 gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt
    32821 atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa
    32881 gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa
    32941 gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta
    33001 gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg
    33061 ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg
    33121 ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta
    33181 gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac
    33241 ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt
    33301 gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg
    33361 aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt
    33421 ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta
    33481 gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg
    33541 ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg
    33601 tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa
    33661 gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc
    33721 gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg
    33781 cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg
    33841 aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa
    33901 ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt
    33961 atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg
    34021 gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata
    34081 tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag
    34141 ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac
    34201 caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag
    34261 aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa
    34321 atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt
    34381 agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa
    34441 gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc
    34501 tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg
    34561 aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga
    34621 ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat
    34681 agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa
    34741 aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga
    34801 attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt
    34861 tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga
    34921 aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga
    34981 aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc
    35041 agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc
    35101 tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa
    35161 tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc
    35221 agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac
    35281 agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga
    35341 agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca
    35401 ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga
    35461 tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac
    35521 tccagcagaa gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa
    35581 tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg
    35641 agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt
    35701 agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat
    35761 aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa
    35821 taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt
    35881 cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa
    35941 acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac
    36001 aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt
    36061 tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg
    36121 gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc
    36181 tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac
    36241 agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg
    36301 tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac
    36361 cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga
    36421 tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc
    36481 gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt
    36541 tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat
    36601 ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat
    36661 gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga
    36721 aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct
    36781 ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt
    36841 agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta
    36901 ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt
    36961 ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta
    37021 tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt
    37081 aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa
    37141 tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata
    37201 atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg
    37261 tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag
    37321 cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga
    37381 ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg
    37441 gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc
    37501 tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac
    37561 atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt
    37621 tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg
    37681 aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa
    37741 tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct
    37801 tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta
    37861 tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat
    37921 ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg
    37981 aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg
    38041 cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg
    38101 tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt
    38161 acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa
    38221 agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct
    38281 gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt
    38341 ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta
    38401 aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata
    38461 cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa
    38521 tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat
    38581 gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata
    38641 aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga
    38701 gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg
    38761 attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct
    38821 ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga
    38881 gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat
    38941 aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct
    39001 tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt
    39061 atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca
    39121 atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct
    39181 acctgataac tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg
    39241 aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa
    39301 taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat
    39361 acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca
    39421 ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa
    39481 acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt
    39541 tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt
    39601 acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga
    39661 gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt
    39721 tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca
    39781 ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt
    39841 tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt
    39901 gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg
    39961 gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt
    40021 gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc
    40081 tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa
    40141 ttggatcatc tcatcaattt tgcatgcggt aatacgaata aatcctttgt gacggacgta
    40201 cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga
    40261 aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat
    40321 ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc
    40381 ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc
    40441 ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa
    40501 atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt
    40561 caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt
    40621 aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga
    40681 aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa
    40741 gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt
    40801 taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac
    40861 acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat
    40921 gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg
    40981 ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag
    41041 tgtatttgta ttatttttga tctctcgaat atcattccta agttcttcta ggatattttc
    41101 ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac
    41161 tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg
    41221 ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa
    41281 tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat
    41341 gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga
    41401 cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat
    41461 aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg actacacaat
    41521 tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga
    41581 tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca
    41641 tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc
    41701 gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag
    41761 tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg
    41821 attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt
    41881 ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg
    41941 cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat
    42001 tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta
    42061 atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg
    42121 caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga
    42181 gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc
    42241 agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg
    42301 tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa
    42361 cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg
    42421 gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag
    42481 acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc
    42541 tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa
    42601 atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg
    42661 tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa
    42721 atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg
    42781 ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga
    42841 aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt
    42901 aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt
    42961 actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt
    43021 atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac
    43081 gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt
    43141 gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac
    43201 gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga
    43261 taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga
    43321 tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa
    43381 acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc
    43441 cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga
    43501 aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga
    43561 taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa
    43621 ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga
    43681 ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat
    43741 tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc
    43801 gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga
    43861 aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa
    43921 atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc
    43981 atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt
    44041 tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa
    44101 acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag
    44161 attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt
    44221 ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg
    44281 agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg
    44341 aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag
    44401 acactagaaa atgaaatgat cagcgaagag catattattc gcgggatggt gggcagagat
    44461 ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa
    44521 gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg
    44581 catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa
    44641 ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag
    44701 gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat
    44761 gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg
    44821 acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta
    44881 actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt
    44941 gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca
    45001 caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat
    45061 gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct
    45121 tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt
    45181 attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg
    45241 aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa
    45301 actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt
    45361 agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa
    45421 tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt
    45481 agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc
    45541 aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg
    45601 tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat
    45661 accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt
    45721 tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta
    45781 tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat
    45841 catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt
    45901 atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt
    45961 actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct
    46021 tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat
    46081 ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat
    46141 cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc
    46201 tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc
    46261 ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat
    46321 tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt
    46381 agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta
    46441 taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca
    46501 agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag
    46561 actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt
    46621 tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat
    46681 gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg
    46741 aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa
    46801 cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga
    46861 ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc
    46921 ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta
    46981 cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag
    47041 aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta
    47101 ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg
    47161 ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg
    47221 ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct
    47281 ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg
    47341 gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca
    47401 tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg
    47461 gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa
    47521 atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg
    47581 aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca
    47641 ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa
    47701 aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag
    47761 ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg cagatacaag
    47821 cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta
    47881 tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat
    47941 agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta
    48001 tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag
    48061 tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt
    48121 ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg
    48181 gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat
    48241 gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca
    48301 gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc
    48361 gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga
    48421 tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga
    48481 attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt
    48541 gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt
    48601 ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga
    48661 agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt
    48721 gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga
    48781 aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac
    48841 ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa
    48901 tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt
    48961 gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc
    49021 gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa
    49081 aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga
    49141 ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg
    49201 ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat
    49261 tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct
    49321 ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac
    49381 agaataattt cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag
    49441 ctgtttcttt tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt
    49501 tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc
    49561 aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga
    49621 tgaaaaataa aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat
    49681 tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc
    49741 atgataattt attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc
    49801 gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa
    49861 aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc
    49921 aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg
    49981 atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa
    50041 cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa
    50101 gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg
    50161 atgtacctca actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag
    50221 gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca
    50281 tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga
    50341 aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag
    50401 caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt
    50461 atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg
    50521 tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg
    50581 tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac
    50641 tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa
    50701 aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga
    50761 cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag
    50821 aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct
    50881 tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag
    50941 cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt
    51001 agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa
    51061 cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt
    51121 cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg
    51181 atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt
    51241 agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac
    51301 aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga
    51361 aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc
    51421 aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat
    51481 gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga
    51541 taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt
    51601 gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat
    51661 tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac
    51721 tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt
    51781 ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc
    51841 acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga
    51901 tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt
    51961 catggcttat caatttaaat tgccggatat cggtgaagga atcgcagaag gtgaaatcgt
    52021 caaatggttc gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca
    52081 aaatgacaaa tcagtagaag aaatcccgtc acctgttaca ggaacggtta aaaatgtgat
    52141 tgttccagaa ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg atgcaccagg
    52201 acacgaagac aatgaagggg acagtggtgt agccgcagaa tcccaaactc cagctaaacc
    52261 ggctgctgaa cccactgttg atacagaatc agctggatcg tcaagcgaag gagtcttcca
    52321 atttaagtta ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt
    52381 caaaccagga gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc
    52441 agtagaagaa attccgtcac ctgtcacagg gacagttaaa aacgtgattg ttccagaagg
    52501 cactgtagca aatgttgggg atgtattagt agaaatcgat gctcctggac acaatagtgc
    52561 accgtcaact agtgcgccat cagctgaagc accaaaagaa aaagtggaaa catcaggttc
    52621 agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta ttggctatgc catctgttcg
    52681 tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta actgcaactg gaaaaggtgg
    52741 cagagtaaca aaagaggaca tcgaaaactt cttggcaggt ggtccaagca gtgcaccagc
    52801 gaaatcagaa gcgccagaag cggcagcacc gaaagaagca gctcctgcag cagaaagcaa
    52861 accagctgca ccagcaaaac cattcaagtc caacttagga gacttggaag aacgtgtagc
    52921 aatgacgcca acacgtaaag ctatcgctaa agcaatggtc aacagtaagc atacagctcc
    52981 acacgtgaca ttgcatgatg aagtagaagt gtctaaacta tgggataatc ggaaacgatt
    53041 taaagaagtt gctgctgcaa atggtacgaa acttactttc ttgccatatg tcgtgaaagc
    53101 tttaacagcg actgttaaga aatacccagt attgaatgca tctatcgacg atgcaaacca
    53161 agaaatcgtt tataaacatt actacaatat cggtattgca actgatacgg atcacggact
    53221 atatgtacca aatgtgaaag atgcagaccg caaaggaatg tttgctattg cagacgaaat
    53281 caatgagaaa gcaaaattag ctcatgacgg caaactatct gcagaagata tgcgcaatgg
    53341 aacaattacg atcagcaata tcggatctgt aggtggcggt tggtttacac cagtcatcaa
    53401 ctatccagaa gtagctattt taggtgtagg aacgattgca caacaaccaa tcgtaaatgc
    53461 tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg atcaccgtat
    53521 cgtagatgga gcaactgcgc aacaagcaat gaataatatc aaacgtcttt tagctgatcc
    53581 agagctatta atgatggaag gatgataaac aaatggtagt tggagatttt gctgtagaat
    53641 tagatacagt tgtaattggt gcgggacctg gtggctatgt agctgccatc cgcgctgctg
    53701 aaatgggaca aaaagtagca atcattgaaa gagagtatat tggcggtgtt tgtttgaatg
    53761 tcggctgtat tccttcaaaa gcactgatag ctgcaggaca ccattatcaa gagtcactag
    53821 attctgaaat gtttggagta actagtgaaa acgtcaaact agattttgca aaaacacaag
    53881 aatggaaaga aaacaaagtt gtccatacgt tgacttctgg tgttggtttc ctattaaaaa
    53941 aacataaggt tgaaacaatc gaaggagaag cgttcttcgt agatgatcac acactacgcg
    54001 tcattcatcc agattcagct caaacttatt cgttcaataa cgcaatcatc gcaacaggtt
    54061 cccgtccaat cgagattcca ggatttaaat tcggaggacg tgtgttagat tctactggcg
    54121 gattagcatt gaaagaagta cctaagaaat ttgtgatcat cggtggcggt gtgattggtg
    54181 ccgaattggg cggagcgtac gcaaacttag gagcagaagt aacgattctt gaaggctcac
    54241 cacaaatttt accaacttac gaaaaagatc tagtaaaatt agtagaagat gatttcaaga
    54301 aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat aacggtgaca
    54361 gcgtgacagt gaaatatgca gtggacggga aagaagaatc cgttactgct gattatgtga
    54421 tggtcactgt aggtcgtcgt ccgaatacag atgatatggg cttagaacaa gcgggtgtcg
    54481 aagtaggaga acgaggattg atcacggtgg ataaacaagg acgtacgaac gtacctaaca
    54541 tctatgccat cggtgatatc gttccagggg cggcattagc acacaaagcc agttatgaag
    54601 caaaaatcgc tgctgaagca atctctggca aaaaagtagc tgtagattat aaagcaatgc
    54661 cggcagtagc cttcactgat cctgaattgg cttctgtagg tatgacgatc aaagaagcaa
    54721 aagatgctgg tatcgaagca aaagcatata aattcccatt ttcagggaac ggtcgggcat
    54781 tatcgcttgg taaaacagaa ggattcatcc gtctagttac gacgatcgaa gataacgtgc
    54841 ttattggcgc gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa ttagctttag
    54901 caatcgaatc aggaatgaat gctgaagata tcgccttgac gattcatcct catccatctc
    54961 ttggcgaaat cgtcatggat gcttcggaat tagcattagg tttaccaatc catatttgat
    55021 ttgaataata caggcgaaac gcaactgaaa aatcagttgc gttttgtttt ttagaattat
    55081 caataatatt attatgtaaa cttaaaaata tgacaacttc ttggtttatt accacttcct
    55141 atgctacact gaagaggaat atgaaatagg agagttgaag atgcaagaat atcagtatcc
    55201 acttgattta gattggacaa cagacgaaat ggtcattgta atgaagatgt gggaagatct
    55261 ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt ttgaagactt atcagcagtt
    55321 taaaactgtt gtcaaatcca ttggtgagga aaaacgattg ggacgggaat ttgaaaaagc
    55381 ttccggttat tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa acaaaacttt
    55441 gagaatgaac gggtgagata aatggaacag atgattgatc atatcaagtc ctggcttgaa
    55501 gaagctggtg agaaaatcaa atcttctcaa caaacattaa caatctcgca aaaatcaaat
    55561 cgaacggatc tagtaacaaa tgtcgatcga aacatccaga attttctaat cgaaaaaatc
    55621 cattcctttg atccagaggc gaaaatattg gcagaagaag aagggtgtaa tcaattatcg
    55681 gatctcgatg gaagagtctt tatcatcgat cctattgatg gcaccttgaa ttttgtgatt
    55741 gaaggggaaa atttttgtat tatgctcgct gtttatgaat ccggcgttgg aaagcttggt
    55801 ttcatttatg acgtgatgag aaatgaactt ttttggggtg gaaaaggaat aggcgtgtat
    55861 aaaaatgatg agccattgcc tagaccagca aataaatcct tatcggaagg cttacttggt
    55921 gtgaatagtt atcttttcgg tcataataaa tatggaatcc gagaaattgc ggaaagcagc
    55981 atggggacac gtatgtacgg atgcgctggt cttgaactag tggcaatctt gaaaggaaac
    56041 cacattgctt atatttcaaa ccttagccct tgggattatg cagcgggaaa tgtcttattg
    56101 gaagagtttg gtatggtaac aagcggtctt tttggtactc ctttgacatt tgaaaaaagg
    56161 gaatactatt tagcagggac ttcttgtgca tatgcagaaa tcgcagacct tttgaaaaaa
    56221 gaaggctgaa aaaaaggtag aaatcattca caaaaatgat aaaaacgagc aaaagattaa
    56281 ttttctcgaa aaaatgaaaa cattttacta tatttcatga aaaaagtctg ctataattag
    56341 cagtcgagta aaatttgccc tgtcataaag aaccaataaa aaaggagtta gttacattga
    56401 attatagaaa cgatatccgt aacgtcgcaa ttattgccca cgttgaccac ggaaaaacaa
    56461 cattagttga tgaactatta aaacaatcag atacattaga cgcacatacg caattgcaag
    56521 aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt ttagccaaaa
    56581 acacagctgt agactataaa ggtattcgcg taaacatcat ggatactcca ggacacgcgg
    56641 acttcggtgg cgaagtagaa cggatcatga aaatggttga cggcgttgtg ttggtcgtag
    56701 atgcgtatga aggaacaatg cctcaaacac gttttgtatt gaaaaaagca ctggaacaac
    56761 atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt cctgagcatg
    56821 tcgtagatga agttcttgaa ctattcatcg aattaggagc agatgatgat cagttagact
    56881 ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag cttgtcagat gatccagcag
    56941 atcaagaacc aaccatggca cctatttttg atacaattat tgaaaaaatc cctgctcctg
    57001 tcgataacag cgacgaacca ttacaattcc aagtttcatt acttgactac aacgattatg
    57061 taggtcgtat cggaatcgga cgtgtattta gaggaacaat caaagtagga gaccaagtag
    57121 cattaattaa attagacgga acagttaaga aattccgtgt aacgaaatta tttggtttct
    57181 ttggactaaa acgtctagag attcaagaag caaaagctgg agacttgatt gctgtctcag
    57241 gtatggaaga tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa gatgcgttac
    57301 ctatccttca cattgacgaa ccaacattgc aaatgacttt cttagttaat aattcaccat
    57361 ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt ttgatggcag
    57421 aacttcaaac agatgtatcc ttacgagttg aaccaacaaa ctctccagat gcatggacag
    57481 tttctggtcg tggagaattg catttgtcta tcttgattga aaacatgcgc cgtgagggat
    57541 acgaattgca agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt gtaaaatgcg
    57601 aaccgtttga acgcgtgcaa atcgatacac cagaagaata tatgggtagt gttatcgagt
    57661 ccttgagctt acgtaaaggt gaaatgcaag atatggtcca tacaggaaac ggacaaatcc
    57721 gtttgacatt cttaacacct gcccgcggtt tgattggtta ttcaactgaa ttcctttcaa
    57781 tgacacgtgg ttacggaatc atgaaccata cttttgacca atatctgcca atgcttcctg
    57841 gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat cgatacagga aaagcaacga
    57901 cttattctat catgagtatc gaagaacgcg gtacagtctt cgtagaacca ggtacagaag
    57961 tatatgaagg aatgattatc ggggaaaaca gccgtgataa tgatttgact gtcaacatca
    58021 cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa agaccaaaca tctgtcatca
    58081 aaaaaccaaa acaattgacg ttggaagaat ctttggaatt cttgaatgat gatgaatatt
    58141 gcgaagtaac accagaaagc attcgcttga gaaaacaaat tttagagaaa aatgctcgtg
    58201 aaaaagcatc taaaaagaaa aaataatctg taaaaggatg aagagagaaa ccagcttgtt
    58261 ataaaaaata gctggtttct ttttttgtcc aactcttttc tgtggagaag tcatctttgt
    58321 caatggacat aaattcagaa aatacgccga aagacgttta aaagactttt aaaaaactta
    58381 tttctagaga gtttttgttt cataataaga taatgtttca cgaaaggaga tcaacttgtc
    58441 cttcgaagaa aatcgaagta aaatggcaag agaaaggagc gggaagatga acgacttatt
    58501 aaaacaatac tttggttatg atgagtttag acctggtcaa aaagaaatta tccagaaagt
    58561 cattgatcag gaaaatgtat taggaatcat gcctaccgga agtgggaaat caatctgtta
    58621 ccagcttcca gctttactat tagatgggct tactgtggtt gtgtcccctt taatctcttt
    58681 gatgaaggat caggtggacg cggcgaatca attaggtata cccgccactt ttatcaatag
    58741 ctcgttagat ggttacgaaa cagctagacg cttccaagaa atcgatagac agcagtaccg
    58801 tttactttat atcgctcctg aacgttttat catgccggat tttatccaag caatgaagcg
    58861 atggaacgtc cgtatgatcg ctattgacga ggcacattgt atctctcagt gggggcatga
    58921 tttccgccca agttatttac agatggcgaa ccagctagat caactgccaa atcgtcctgt
    58981 aattgttgct ttgacagcta cagcgactgt gcaagtagct gctgacataa aaagactttt
    59041 aaaaattcca gaaaacaacc atattcagac tggttttgaa cgagaaaatc tacgatttca
    59101 agtaattaaa gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga tcaacaaaaa
    59161 tcaatctggt atcatttatg cagcaacgag aaaagaagta gaccgtctct atcatttgct
    59221 taaaaaattc gatttttccg tgggtagata tcatggtggt ctaaatgaga atgagcgaac
    59281 agaaatgcaa gaagcctttt tatacgatcg cttgcagctg attgtagcga caaatgcatt
    59341 tggtatgggt ataaataaaa gcaatgtccg ttttgttatc cattatcaaa ttcctggctc
    59401 tctagaagca tactatcagg aagctggtcg ggcgggaaga gatggtcttt ccagtgaagc
    59461 catcctgctt ttctctcctc aagatatcca agtccaaaag ttttttatcc agcagtccca
    59521 aagagaagaa gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga cagaatacgt
    59581 gtatattgag tcctgtcttc agcaatacat cctaaactat tttggcgaga caagcagtcc
    59641 gtgcaaccgt tgtggtaact gtttagatga cagagaaata gtagaagtaa cgacagaagc
    59701 acagatggtt ctttcttgct taaaacgtat gggtgagaac tatggtaaac aaatgctgat
    59761 gaaagtctta gcaggatcga aagaacaaaa gctgcaagct ttaggttttg gacatttatc
    59821 tacctatgga ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa ttgaatattt
    59881 gatatcgaat ggctatcttc tgaccatcaa tggtgagtat cctgttttga aagtaacaga
    59941 aagagggata caagtattga aaggacaaga atctgtttat cgaaaggaac ccaagaaagt
    60001 ccagcagcta tcggatgaag aaaccgatac actatttgaa gtgctgcggg aactgcgaac
    60061 ggatctagca tcagaagcag gcgttccccc gtatgtcgta ttttcagatt ccacattgaa
    60121 agaaatgagc cgaatacgtc catcaagcag attggagatg cttcagataa aaggagttgg
    60181 ccaaagcaag cttgataaat atggcgaagc ctttttatct cggataaaaa atgcggactc
    60241 taacgttctg gataagtaga aagaacgaat acgtaaaaat gaataaaaac aactggttaa
    60301 atctgttttt tttgctcctt atgaaaatca aatatgttat aattaaggaa ttagaatggg
    60361 aggtaagcac agatggattc tttgtcgaaa aaacttgcga tccaagtatt aaatgatgaa
    60421 gcagaacgga tcaaacgatt aatccgaaat caaaacaaca gtctctgtat ctcacaatgc
    60481 aaagccttcg aagaagtggt agatacacag atgtacggct tttcccggca agtcggtttt
    60541 gctgtccgta taggagtaat cggaaagaac gagggccagc tgatgttaag tgagctagaa
    60601 cgagaattga atcagttata tagtgaggtt taccaagaaa tttatgataa aaaagaagtt
    60661 ggcaaggagg aatagacttg cctaagaaag tgaaaaaaag gcatctatta gactatagca
    60721 tcttgattcc ctatttaatt ttatgtgtat tgggattgat catggtatat agttctacgt
    60781 cgtatctatt attagaaaac ggacaaaatc cgtccgcatc agtcattaac cagagtattt
    60841 tttgggtgct tagcttgatt gctatcgcac tactttataa aatgaaaaca gatgttttga
    60901 aaaaccagcg tttgatcatg gcagccatcg cagttttgac gattttgctg ctgattgttg
    60961 tgttttttgg taaggagatc aatggggcga aaggttggct gcaaattgca ggattctcga
    61021 tccaacctgc cgaataccta aaaatcattt ctatctggta cctgtctctg actttatcta
    61081 aaagacaaaa cagtgtacaa aaagattttc ttgggactgt gaaacgtcca cttgcaatgg
    61141 ttatcggact tactgcatta gtcgctattc ttcccgattt tggtaatgcg gcagttatct
    61201 ttttgattat tttggttctg cttttagcca gcggagtcaa ttatgtgtat actttgattg
    61261 taggagtagg aggcttttgt ctaagtactt ttacgatctg gctgatcaat atcacaaacg
    61321 gaaagatctt acccggacgt ttacagtata tctataatcg atttgctatt taccaaaatc
    61381 ctttctcaga cgaattaaat aaagggcatc aactagtcaa cggctactat gccatgttca
    61441 acggaggact atttggcagg ggattaggta acagtatcca gaaaaaagga tttttacaag
    61501 aagcacaaac cgattttatt tatgcaatcg tagtggaaga actgggagtg attatgggga
    61561 ttttgatatt agcgttgcta ttctttatga ttgtgcgcat tgtccttgta ggtatccgtt
    61621 caaaagatcc gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt ttgatccaag
    61681 tatttgtcaa tcttggagga attactggcg ttattccttt gacagggatc acgttcccat
    61741 ttttaagtca aggaggctcc agtttgctga tgctttcgat ttgtgtcggc tttgtcctca
    61801 acatcagtgc ggatgaaaaa cgcaagagct taggtttata ataaaaaaag atgtaggagt
    61861 ggttcgatga aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg tgtatttcgt
    61921 gcttgtacag aattaggaat taaaactgtt ggaatctatg cagaagaaga tgaatattcc
    61981 gtacaccgtt tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa accgattgat
    62041 gcgtatctag atattgaagg gatcatttct atcgcgaaag aatgcggagc tgacgctatt
    62101 catccgggtt acggtctttt gtcggaaaac ttgaattttg cgcaacggtg cgaagaagaa
    62161 ggcatcattt ttgttggacc caaactgcat catttagata tttttggaga taaaatcaaa
    62221 gcaaaagctg cagctatcga agcggggatt gcttcgattc caggcacaga tggaccaatc
    62281 gcttctatag acgatgcctt ggagtttgcg aaacaatacg gttatccgat tatgatcaag
    62341 gcagctctag gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa gagtgcaaga
    62401 gagggctacg aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga tgaagtgtat
    62461 gttgaaaaat acatcgctaa tcctaagcat atcgaagtac agattctcgg ggatacgcat
    62521 ggaaacgtca tccatctttt tgaaagagat tgttctgttc agcgccgtca ccaaaaagta
    62581 gtagaagtag cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat ctgccaggca
    62641 gctgtccaat tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga atttcttgtt
    62701 gaaggagatg atttttactt tatcgaagtc aaccctcgcg tccaagtgga acatacgatc
    62761 acagaaatga ttacagatat tgatatcgta acaacgcaac tgttgattgc tcaaggtttg
    62821 gatctgcata aagaaatcgg attgcctcag caagaaggaa ttaaattaaa tggttcagcc
    62881 attcaatgcc gaatcactac agaagacccg ttgaataact ttttaccaga cacagggaaa
    62941 atcgatacgt atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg aaatgcttac
    63001 gcgggctatg tagtgacacc gtattttgat tcattgctag taaaagtatg tacgcatggt
    63061 gctacctttg aaacagccat tcagaaaatg gaacgttgct tgaaagaatt tagaatccgt
    63121 ggagtgaaaa caaacattcc atttatgctc aatgtcatca cacatcctga attccaatca
    63181 ggaaatgcaa aaacaacctt cattgatagt acagcaacat tatttgaatt tcctcgacta
    63241 cgagaccgcg gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa tggttttcca
    63301 ggtatcgaaa gtggcgaaaa accattttat gaagaaccac gtatgcctaa agatctaatc
    63361 acaagaactg attacgtaac ggctaagaat gtattagatg cgaagggtgc cgatgctttg
    63421 gtcgaatgga tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt tagagacgcc
    63481 catcaaagtc tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat tgcacgtttg
    63541 actggagaag gtcttcctga actcttttca agtgagatgt gggggggagc aacatttgat
    63601 gtggcttacc gctttttgaa tgaagatcct tggcaacggt tgagaaaaat tcgttcttta
    63661 atgcctaata cgttgctgca aatgcttttc agaggttcaa atgccgttgg gtattctaat
    63721 tatcctgaca atgtattggt ggaatttgtc aaagaagcag cagcgcaagg aatcgatgta
    63781 tttcgaatct ttgatagttt gaactggact ccacaaatgg aaaaaagtat ccaagctgta
    63841 cgggatactg gtaaaatcgc agaggcagct atttgctata ctggcgatat caacgatcca
    63901 agtcgggcaa aatacaatgt tcagtattat aaagacatgg caaaggaact agaacaactg
    63961 ggtgcgcata tcattgcaat caaagatatg gctgggctgc ttaaaccgca ggcagcttat
    64021 cgtttgatta gtgaattgaa agaaacgaca gatctgccaa tccatctgca tactcatgat
    64081 accagcggga atggaatcat cacttattct gctgcttcaa aagcaggagt agacatcgtt
    64141 gacgtggcaa tgagtgcaat gagtgggaat actagtcagc ctagcatgag cagcttgtat
    64201 tatgcactgg tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc acagaaatta
    64261 aaccattatt gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg cttgaatgct
    64321 cctgaaacag aagtatatat gcatgaaatg ccaggaggac agtattcgaa tctacagcaa
    64381 caggcaaaag ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat gtatcatact
    64441 gtcaacttga tgtttggcga tatcgttaaa gtaactccat catctaaagt agttggagat
    64501 atggcactat ttatggtcca aaatgatctt acagaagaag atatttatga aaaaggcgaa
    64561 acactaagtt tccctgaatc tgttgtgact ttcttccaag gagaactagg gcaacccgtt
    64621 ggcggatttc ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc attgaatgaa
    64681 cgtccaggtc ttttggctga atcagtcgac ttcaacgaag taaaaaaaga attggcagaa
    64741 aaaattggct atgaaccaaa acaagacgaa gtattaagct atttgatgta tccgcaagtc
    64801 tttttggact atcaaaaagc atataatcaa tttggcgatg ttacattact ggacacacca
    64861 acatttttcc aaggtatacg tttgggtgaa acaatcaatg tacaaatcga acgcggaaaa
    64921 acgcttatca ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa tcgcgtactg
    64981 ttcttcaatt tgaatggaca aagacgtgaa atcactatca aagataattc gatcatcagt
    65041 acggtccaaa cccgattgaa agctgaaccg acaaaccgag aacaaattgg agcaacgatg
    65101 tctggttctg ttttggatgt attagtgaaa aaaggcgata acgttaaaaa aggtgatacg
    65161 ttgatgatta ctgaagcgat gaaaatggaa acggccatcg aagcacgttt tgacggagaa
    65221 gtcgcacatg tatatgtgtc ttcaggagac actatttcgt ctggtgacct tttgattgaa
    65281 gtgactgaga aataataagc gaaaggtggg gaaacatgaa aagactgggt ttgttttggc
    65341 tgattctgct attagttatt gtggcattct acatggaacc agtcttgttt ccgccaaaac
    65401 ttgaagtgaa ggaaaactct gatcaacaaa tatcaaacca aatgacaacg tggaaatacc
    65461 aggagcttaa agcaactggt ttttctactt atatcggaaa accgatcgat cagctggaaa
    65521 aggattttgg ccaagcctat gatcgattaa ctagcggatt tggttttgaa acaagatatt
    65581 atgaaaataa agctagtcat ctcacatttg aagcgaacat ccaagacagc agagtcacaa
    65641 ctgttaaagt actgcaaaca gggcctgctg atatcgcgcc gttttattta gggatgacga
    65701 tgcaagatct tacgaaaatc actactatct atactaattt cacttttgag taccatgata
    65761 cagaagttgg aatcgaacta atggaagagg atatgaatta tcgtccgcta attgcttttg
    65821 ataacgacac ttttgctatt ttattttttg atcaaacaac aaacggccta ttttcagttg
    65881 tgtatttaga taaagatagt cttttgaaac tattgccata tcaagtgttt ggtgaaggcc
    65941 ttccacatta tcaagcagat aaatcggctg attgggaatc aatcaatcgc gttaaagaac
    66001 gaaaaagtac gactttatta aatatgctta gaagagaaga cgaacttccc atatacaatc
    66061 aaacacttgc ctttcaaaac cacagtcagc tgctactaca tgattttttg acaaggcctg
    66121 aggaaatatt gtcagaggaa cgtatggctg attggcaaaa atcgctaggc agtgctcaaa
    66181 cgacagtgaa attctcactg acgaatgatg aattggagaa gttaatcagt aaacaaaaat
    66241 tagaaaatac tagtgggcta tttattcatc cggcgattga cgctactttt tcgtttttat
    66301 attggtacag cgatccttac aaccatgacc gtttcatgga cccgggaacg gattctgtag
    66361 gcattgcttt ttctaaagaa aatatgttag tcttattgca agaagaaaac ggagaaagta
    66421 gtgatagcag tgatcgttaa cgaagagctg tttcaactgg aggatcaatg cctccaagtc
    66481 gctgaaatga taaaaaagag taaaacgatg gcttcttatc ttgaacataa acagtcaatg
    66541 gaagaatctg caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa agaagcattt
    66601 gaaaatgtag cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa acgagctgtt
    66661 cgaaaagcaa aaagacagct tgatatggat gagcatgttg cagctttccg atatagtgaa
    66721 acacagctgc aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga agtttctaat
    66781 gaaatcaaag tagatgcagg tagcccgttt tttgaaacga aaaaacactt tggatgcgga
    66841 ggtaattgtc atgcaattag acaatagtga atttgaaata acaaaacgac gcgggctgat
    66901 cgtatgggtc tacagcttaa aacaattaaa gaatctgaaa aagttcggct tgttgcatta
    66961 tgtctcaaga agaatgaaat atgtcgtgct ttacttaaat gaagagacat tggaagaaac
    67021 agaagaacga atccgtaaat tacattttgt tcgaaacgta gaacgttctt accgaccgga
    67081 tatcgaaatg aattttgccg aaaaaatagg aaacaaaaca gtattaaaag acgaaggttt
    67141 cgaaattgaa gagctaaata cagaaatccg attagctgaa catctttaaa aatctcttat
    67201 gagcagtttt taaataaaaa ataacttgga taacgatcga tttttgatca ttgtccaagt
    67261 atttttttgg tatactagta ccagtaccat ttctaaggag tgagtagatg cggataatat
    67321 caggaaatta tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc agaccaacta
    67381 cagataaagt aaaagaatcc atattcaata tgattggacc atattttgat ggagagacag
    67441 tattggatct gtttgcagga agcggtggtt tagctatcga agcagtatct agaggctgca
    67501 gtcatgcggt atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa gaaaacatcg
    67561 aaattacgaa agaaccagag aaatttacca caataaaact tgatgcggac aaagcaatcg
    67621 aacggctagc agcggagaaa caacaattcg actacttgtt tcttgatccg ccatatgcaa
    67681 aacaaaaaat aatcgatcaa atccataaaa tggaagaact gggacttttt tctaaaaccg
    67741 cagttatcgt atgcgaaacg gataaaacag tcgaattgcc agaaactgtg gctggattcc
    67801 atcaaataag aaaacagatt tatggcataa cggccgtaac tatctataga aaagagggaa
    67861 atcaatgaaa cgtgcattat tcccaggaag ttttgatcct tttacaaaag gtcacttaga
    67921 tacggtggaa cgtgcggcta aattattcga tgaggtagta attggtgttt ttatcaatac
    67981 ttcaaagaaa agtttgtttc ccccagaaga aagaatgaca ttgatcacaa aagctgtttc
    68041 tcatcttcct aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag aaacagcaaa
    68101 agagattggt gcagatgcgt tgatcagagg catacgaagt atcaaagact ttgaatatga
    68161 acgagagatt gctcagatga atcatcatct atatgatgaa ttggaaactg tcttcttatt
    68221 agcaaaacca gaatatagtc acgtgagttc cagtatttta aaagaagtac tgcactttgg
    68281 cggagatgtg agcagctact taccgccagt gatcaacgaa gctttagctg gaaagcgtga
    68341 atcaaatgaa agctaaacaa tggttgaaaa cagtactgat tatcatattt ggaatgttga
    68401 tcattggatt tgtagtagtt cccttgccgt attatgtaga atcaccggga gccacgatcg
    68461 atctgaaaga actaattacg gtcaatgata aggaagataa aggttctggg tctttctctc
    68521 ttacctctgt tggtatcagg caagctacag gtttcttagc tttaaaagct aaaatgaccc
    68581 cttttgaaga attgatcagt gaagaagaat tgactggtgg ggcgacaaat gaagaatata
    68641 atcaaatgca acagtattat atggattctt cccaaaatgc agcaatcgaa caagcgttaa
    68701 aacttgcaga caagccttat aaaatggaat tcaaaggcgt atatgtcatg agtgtagaga
    68761 aaacatcgaa tttctttggc aagctttcag ttggagatac agtaacaaaa gtagacggga
    68821 aaagctttca aagtacagaa gcatttatgg attatgtaaa aagccaaaaa gttggacaaa
    68881 caatcgaaat cagttatttg cataatggtg aagaaaaaac agcttctggt gatctgatcg
    68941 agttaccaac agataaaaag gcaggaatag gtatcacgct tactgaccat accgagatcg
    69001 aatcagatac agatgttcgt attaattctg gctctatcgg tgggccatca gcgggattga
    69061 tgttcacttt agaaatctat gaacaagtca ctggtaaaaa cttgcgacat ggtaagcaaa
    69121 ttgcaggaac tggaacgatc aacagtgaag gagaagttgg ccgaatcgga ggaatcgata
    69181 aaaaagtggc aagtgcggat aaagctggcg tagagatttt ctttgcacca gatgatacga
    69241 tttcagacga tgttaagaaa gaatatcctg aaataaaaac caactacgaa gaagcaaaag
    69301 ctgccgctaa aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca gtacaagaag
    69361 cgcttgacta tctagagaaa atgaaatgaa actaaataat taaagagggg ttgtgacacg
    69421 agttctgtca caacccctct ttttcgtcaa aaatcttcac gataaaaata aaaacaaaag
    69481 aaaaaattga cgtaattaga tgttgaaggg acgattttgc cagagctcac tgtttcttca
    69541 attttaaatg aaaggagtaa tgaatgattt ttactggtca ttctttgaaa aaagaacaat
    69601 gatagaaaaa atcagcaaga aaatactagc ttcacccaaa ttgatcattt ggatgtctat
    69661 gatagttctt atcttttgta tcgcaagtgg ttttttatta tcaaatttgc tattttatag
    69721 agaaacagaa gagactgctg caaaagaaat actttcttcg tttagttcca gtggagaaga
    69781 tccatcagct gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca tgtacaccga
    69841 tatcaaagga agtgtgaagg agcctggtat ctattccttt tcttcagaag aacgggtcta
    69901 tgatgtacta aagcgtgcag gtggattatt agaagaagcg gatagcgatc gaatcaactt
    69961 ttctgcgaag atagaagatc agcaagtcct atatatccct gctgtaggtg aagagctgcc
    70021 agaacatctt gatcagttgg catcctcgga aggaaaacag cctacagcag attccgagcc
    70081 tcccaaaata aatatcaata cagccactcc atcagaactt cagcagattc ctgggatcgg
    70141 atcagtcaaa gcgcaagaaa tcattcgatt tagagaagaa aacggatcct tccagaaagt
    70201 ggaagacctg caggagattt ctggtatcgg tgaaaagaca gttgaaaaac taaaaaactt
    70261 cgttacaatt aaatagaatg attcaaaatc gactggcacg agcaataaat ggacgctaat
    70321 ggatactaag aataatccaa ttctaagagc ttggcaggac gaggaggtag tatttttgga
    70381 taggaaatag cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg aatgtctatc
    70441 tccaaaataa aatatctggg attcatatta tgaatacggc tgtcagcggc aataacagtc
    70501 aagctctacg agaacgcttt gccaaagatg tacttagcta tacagcagat aaagtattta
    70561 ttctcattgg gacgaatgat ctagcagagc ataaacaact ttctaaagaa acgtaccaaa
    70621 aaatctgcag tggatgatag ttcagctgaa aaaagactat agttctgatc atgctttctt
    70681 tatcacgcca ccagcagtcg atgagacaaa acaaaaatat cggaataaca agttgatcaa
    70741 agaatatatc tctgttctca cagaagtagc aaaacgccat gattgtgaag tatcgatttt
    70801 tatacggaat ttcttgaaca aatggattcg aagaattttt ataatgggct aatggatgac
    70861 ggacttcatt tcggcagtga agggtaggat cttttgtcag acaggatgat tcgttatatt
    70921 taaagaatat tttgtttaag caatgagtac tttttaatca acttgaaaaa acggaggaat
    70981 ataaaacatg ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa aagttactga
    71041 taagtcacat aatgaacgca ttccttggga ccagtacttt atggctcagg cagtactgtt
    71101 atctttacgt agtacgtgta cacgtttaga agtaggagcg acactcgttc gagaaaaaag
    71161 gatcattgct ggcggctata atggagcagt cagtggagat gtccactgca tagatgaagg
    71221 gtgttacgtc gtggatggac actgcttgcg aacgatccat gcagagatga atgcactcct
    71281 acagtgtgca aaacttggta tcccgacgga aggttcagaa atctatgtca cacattttcc
    71341 atgtttggcc tgtacaaagg ctttgttgca agctggtgtg aaagggatca attatctcca
    71401 tgactatcga aatgatcctt atgcccaagc gttgatcgaa caattaggtg tatctgtaca
    71461 ccaagttgct ttagactcac agcatttttc aaaacttcaa aatgaattaa caggaaaaca
    71521 tgtctagcgt tttagaaagg catgttcgaa atcggttgat ttttccggca attacgtcga
    71581 tgattatctg tttgttgtat tacactgaca attggggtta tcgtttgctt cttgccggaa
    71641 tttttttatg gttttcgcta aaagaaggac gttttatcgc gtacttgtgt attttgtgta
    71701 gcataacggt atccatttct tataggctac aagagtattc atttttagag gaaaaaacaa
    71761 catctgcgca aattactgta tttacagata cgattcgtgc aaatggtaat tttttgacaa
    71821 tggaaggaag gttatcagag aataaacaaa agctccagct ttcttatcaa gtaaacacag
    71881 aagcagaatt gaaaacatgg cttaacttcc gaggaaaggg aatagaattg actgctgaag
    71941 gcgagttcat ctctccagca aaaaagcgga atctttataa ttttgaccaa gaagcttact
    72001 tcaggaacca tagaatttct ggacggttcc aaattcaaaa gattgaacag atcaccatgc
    72061 aaaatcactg gcgaaactgg cttcagagaa aaagaggatc ttttatctcc tttgtccaat
    72121 ctgaatttcc gcaaaaaaca agtgtatata ttaattcttt attatggggc tataaagatg
    72181 caggattcag agaaccagaa gaaattttta gggagagtgg tttgctgcat ctgttcagtc
    72241 tatcgggtct acacatccaa ttttatttag ggtggctgta ttatttattt cgacgtatcg
    72301 gatggccact acacgtcagt attattccgt tgagtctact aactatctgt tatgtttcta
    72361 ttgcaggaag ttctatttct gttttgcgtg ctagtttgct gtttttattg cgattgctag
    72421 tcaaattgct gaataagcat tattccacca tggacttatt tgcagttgtc ttttggcttc
    72481 ttttactatt tgatcctcga tttctttttc atgcgggtgg acagcttagt tttttcatga
    72541 gttttttgtt tatcctgatt tcttttgatg gagattggtt gaaaaaaacg gcctctcacg
    72601 tcctgtttac tatgatcacg atgccgttga ttgtatggca tttttatgag tggcctattt
    72661 taggtagtct ttttacatta ctgattgcac cggtattcaa gtttcttttt cttcctggtg
    72721 tgttatttct cacactcttc aatcgctggc tgccggaaac tttacttttg ctgatagaag
    72781 agggattaat cttatttgag cgaataatcg atttttcttc agggtcgtct ctcattatag
    72841 gacgtttgcc ccttgtgcta tctgggatgt atattttcgg attactgatt tgtatggata
    72901 ggttgaaaga acaacctgtg aaacacttgt tttatgttgg tcttttttct tttatttttt
    72961 tgactttgcc atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc ggtcaaggag
    73021 acagtatctt tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat actggaggtc
    73081 agttacactt tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct gctgaaagaa
    73141 atatcgttcc atatctaaaa ggtcagggga taagtgtaat cgataaactc atcttgacac
    73201 acgacgatac ggatcatgtg ggagaattac ccacgttatc tcagcatttt actatcaaaa
    73261 aaatttatat cggttggggc gctggacgaa atgagccatt acaaaggcat ttatctgaag
    73321 ctcaaaaaaa tggtactgag atcattgaaa tcaaacatgg ggacaggatc agcggttatt
    73381 atgatcttta tgtattgact ccttttgaaa agggagaggg aagaaatgaa gattcgattg
    73441 gtctatggat ggaatataac aataggcgct ttttattttt aggagatttg aatcaagcaa
    73501 tggaaaaaca attgcttttg atttatccta atctgaaagc cgatgtagtc aaattaggtc
    73561 atcacggtag ccgtacgtca agtaacacag attttctttc ccatattgaa gcaaaacatg
    73621 ggattatatc ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa gtattggaga
    73681 cactagaaga aaatcagata aaaacatggc gaacggatca acaaggaatg atcacctatc
    73741 gctggcatcc agtttttcat cctcatgggc tagtggaaac aatgatagac tagtaattct
    73801 catctgctca tggtaggata atttttgaaa gtggtgagta atttgagtat acaagcagag
    73861 ttacaaaaaa tacgtaaaga aaaacttgca tcttgttact tgattttagg aacagaaaag
    73921 tttctccaag atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga gggcgaagat
    73981 gacttaaatt ttttaagttt tgacatggaa aatgcaagtt tagatgaggt agtagctgaa
    74041 gcagagacgt tgccgttttt cggagatcaa cggttagtat tcgtagagaa tccttatttt
    74101 ttaacaggtg aaaaagtaaa caatggaatc gaacaaaata ctgatttatt gacggcttat
    74161 ttgaaagaac cattagaatc gactgtatta gtattctttg cgccatatga aaagttggat
    74221 gaacggaaaa aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa tgtccaacag
    74281 ttgaatgaaa aagaagtacg tcaatatctg atgaatacct tagagaattc ctcaatcaaa
    74341 atggaacgaa gtgcaattga tttattcttg aggttgaccg acttagattt atccaaaatg
    74401 atgagagagt tacagaaatt ggtcctttat ggacaaaatc aacagcaaat cacggtaaga
    74461 gaagtagaag aattagttcc taaaacgtta gaacataata tttttgacat gacccaatat
    74521 attttaaaag ggaagacgga acaagctttg cgtttatacg aagatttagt tatgcaaggg
    74581 gaagaaacaa tcaaaattaa tgcgattttg ctttctcaat tacgtttatt tttacagaca
    74641 aaatttcttg taaagattgg ttatcagcag gcaaatattg ctgaaacatt gaaaatccat
    74701 ccatatcgtg taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca tttgctggta
    74761 cagttgtttg atcaattagt tgagatggat tatcaaatca aaacgggaca aatcgaaaaa
    74821 gaattaagtt ttcaactatt cgttttgcgc acagggcagt tgttaagaaa tcgataagaa
    74881 gaaagacgag ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt ttgacaaata
    74941 aaaaaatcgg ccgaagccga ttcttttatt tagcgatttt tttgcttaaa cgagatttgt
    75001 cgcggttagc tttgtttttg tggattagac cttttgattc agccatatca atagctttaa
    75061 cagcttcttt atataacgca tctacgttgt cagcacctga agctacagca tcttcaaatt
    75121 ttttgattgc agtacgcata gcgcttgttt gagatgaatt tttcacatta gcgttttcgc
    75181 ttgtacgaac acgtttgatt gcagattcaa tgtttggcat tagtttcacc tccgataact
    75241 taagttctta cgcgtgttac cgcataattc aacattcatt attataccta aaggcagcgt
    75301 gctttgcaat atgaacatga caagtttttt tccttaacag tcttttatta ggattttttt
    75361 tcagtaaaaa cggatagtac aagctgaaaa aaatcaaaaa gacgctattg tgggaggcaa
    75421 tagcgtcaaa ccaaactaga tttttattta tctatttaaa caaagtttga atgggaatgt
    75481 ttggtactaa gttattatat aacaaagaga accgtttgca aagttttttt tgaaaaggga
    75541 tacgattccc tgttgttctt tgtttttcta ttaagaaact gcagttattt tataaccagt
    75601 atcctttgat cagtaaaggc tattctggtg taccgattat tttaatgtat tcatcgatat
    75661 tttttatcaa catttttttt atgaccaact ggttccgacg gttccatttg ccacttactt
    75721 ccagcacata tttatttagg ggcaaacgta agatatattt tgccaaatag cgatctttaa
    75781 gcaggccatt gatcacacgg tcctcaagct gcaagttgat cccagtcagc atcattggct
    75841 gttcttgtaa aacgtacgta tcaattaaaa gaccttcaaa agtttccaca agcgtcttcc
    75901 tttcttttca aagtaacctg aaaacaggca ctaattcttc cattttgata ttcgttgaag
    75961 tggatgcgat cattctttct tttgtccact gtaatctagt ataacaaaaa agagcagaag
    76021 agccaaaaag aaaaaagagc atcgaattct ataggaagtc tcattggcac ggcctaaatt
    76081 attacaaaac attgattaaa aaaagcccct gcatcgcagg agcatcccct atatcgtttt
    76141 aataggtcaa tcaaaaagca gtatcaaaca cattttgtat caaatcagga aataatagat
    76201 aagctaagcc cagaatcaaa agtaccgcta gaagaaacca gccgattgtt cctatgaaat
    76261 actttaattt ttcgaacatt gtatcacctc gttgtcagta tatcacggat cagtaaattg
    76321 ggggtgttaa gaatctccta aaatagacaa aatttgttct attttcctaa agcaaatact
    76381 tgtaagatac agtaagtgat accagaagca atgaccggtc cggcggcgat tcctcttaag
    76441 aagacaacac ctaaaatcgt gccgaaaaca agtgcaacag tgatttgtgg atcagaagca
    76501 atcaatccta cacccttgga agataagaca gccactagcc caccgcaaaa aacagctacc
    76561 cagccaatag gggatttaaa ggcttctaat agatcacgta gaccgatttc tcctgttgcg
    76621 atagggatta gaatagccgc tgtgatgatc gtcaccccaa tattgattcc tttgccttga
    76681 agccaaggga aaaaggaacg ggaggctggt atgagcttca taatcaaaac aaaagctgcc
    76741 gctatcatca gactttgatt tttagctaaa attgctacaa gtaaaataat tcctaaaaaa
    76801 agccaacttt ccatgaaata tattcctcac tttcttcgaa acaatcataa catgttttta
    76861 gaaagttaca aaagtaggaa ttcatccttc aagacctatt cataagctgt ttttttcata
    76921 aaataaacaa aatcataaaa aaacactttg cttatttgac agaagacagc cgcgctttgt
    76981 atagtggaca agacttacat ttttttgaag aatcagaaag atcaggtaaa ggtcttagta
    77041 aacgcaagcc attcagaata accaatattg tgcttccttc gtgtcctaca actccaagag
    77101 gtagatttaa aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa
    77161 aaataatatt ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa
    77221 atttatttaa gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc
    77281 cttgccccat agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat
    77341 caccaaccat cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt
    77401 cttccggagt acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg
    77461 ctgtgccaga atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg
    77521 aaattacttg ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact
    77581 gctgatcttt agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt
    77641 ccgcaatctc tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc
    77701 attgcgcacc taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt
    77761 attggatttc ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat
    77821 gcgtcgtttt ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga
    77881 aaaagctgtc agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa
    77941 tcgcttttaa ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt
    78001 ttgcagaatt tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag
    78061 gagaagccac aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta
    78121 ggaaaaagtg ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat
    78181 tattttcgat tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta
    78241 ctaattggat aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga
    78301 tgggatttcc ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag
    78361 gcacagattc accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc
    78421 catctatcgg aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag
    78481 aaacatctac ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa
    78541 gattcatcaa agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac
    78601 cgcttaaaca aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg
    78661 caccaatagc agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt
    78721 ctttcaagcc ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa
    78781 gtggggtgat ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc
    78841 agccgattgt tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc
    78901 aaaacaagga tagcataata aaaatgtaat tgataataat aacagctaaa tgagaacgat
    78961 aatggttatc atttggtgat taagcttgat gttgttttta ttatttataa tgattattat
    79021 taaagaaaat aaaaaaccag aacatttctc aaataaga
//
LOCUS       DANJLF010000007        64981 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000007 DANJLF010000000
VERSION     DANJLF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            276..596
                     /locus_tag="PWT25_000546"
     CDS             276..596
                     /locus_tag="PWT25_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL1020655.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            750..1655
                     /locus_tag="PWT25_000547"
     CDS             750..1655
                     /locus_tag="PWT25_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1020656.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1652..2425
                     /locus_tag="PWT25_000548"
     CDS             1652..2425
                     /locus_tag="PWT25_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1020657.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2606..4030)
                     /locus_tag="PWT25_000549"
     CDS             complement(2606..4030)
                     /locus_tag="PWT25_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1020658.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4241..4687
                     /gene="nrdI"
                     /locus_tag="PWT25_000550"
     CDS             4241..4687
                     /gene="nrdI"
                     /locus_tag="PWT25_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL1020659.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4752..6734)
                     /locus_tag="PWT25_000551"
     CDS             complement(4752..6734)
                     /locus_tag="PWT25_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1020660.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6748..7644)
                     /gene="murQ"
                     /locus_tag="PWT25_000552"
     CDS             complement(6748..7644)
                     /gene="murQ"
                     /locus_tag="PWT25_000552"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL1020661.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7647..8708)
                     /locus_tag="PWT25_000553"
     CDS             complement(7647..8708)
                     /locus_tag="PWT25_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL1020662.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8919..9485)
                     /locus_tag="PWT25_000554"
     CDS             complement(8919..9485)
                     /locus_tag="PWT25_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1020663.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9513..11015)
                     /gene="eat(A)"
                     /locus_tag="PWT25_000555"
     CDS             complement(9513..11015)
                     /gene="eat(A)"
                     /locus_tag="PWT25_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL1020664.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11273..12394)
                     /locus_tag="PWT25_000556"
     CDS             complement(11273..12394)
                     /locus_tag="PWT25_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL1020665.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12520..13461
                     /locus_tag="PWT25_000557"
     CDS             12520..13461
                     /locus_tag="PWT25_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL1020666.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13611..14507)
                     /locus_tag="PWT25_000558"
     CDS             complement(13611..14507)
                     /locus_tag="PWT25_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL1020667.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14776..16116
                     /locus_tag="PWT25_000559"
     CDS             14776..16116
                     /locus_tag="PWT25_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1020668.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16334..16777
                     /locus_tag="PWT25_000560"
     CDS             16334..16777
                     /locus_tag="PWT25_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL1020669.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17281..18699)
                     /gene="nhaC"
                     /locus_tag="PWT25_000561"
     CDS             complement(17281..18699)
                     /gene="nhaC"
                     /locus_tag="PWT25_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL1020670.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19102..20325)
                     /locus_tag="PWT25_000562"
     CDS             complement(19102..20325)
                     /locus_tag="PWT25_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL1020671.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20356..21195)
                     /locus_tag="PWT25_000563"
     CDS             complement(20356..21195)
                     /locus_tag="PWT25_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL1020672.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21212..21892)
                     /locus_tag="PWT25_000564"
     CDS             complement(21212..21892)
                     /locus_tag="PWT25_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020673.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21894..22967)
                     /locus_tag="PWT25_000565"
     CDS             complement(21894..22967)
                     /locus_tag="PWT25_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1020674.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23023..23261)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23304..23537)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23614..24285)
                     /locus_tag="PWT25_000566"
     CDS             complement(23614..24285)
                     /locus_tag="PWT25_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1020675.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24372..27050)
                     /gene="mgtA"
                     /locus_tag="PWT25_000567"
     CDS             complement(24372..27050)
                     /gene="mgtA"
                     /locus_tag="PWT25_000567"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL1020676.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27709..28146
                     /gene="ndk"
                     /locus_tag="PWT25_000568"
     CDS             27709..28146
                     /gene="ndk"
                     /locus_tag="PWT25_000568"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL1020677.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28168..29034)
                     /locus_tag="PWT25_000569"
     CDS             complement(28168..29034)
                     /locus_tag="PWT25_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1020678.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29194..31287)
                     /locus_tag="PWT25_000570"
     CDS             complement(29194..31287)
                     /locus_tag="PWT25_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL1020679.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32036..32659)
                     /locus_tag="PWT25_000571"
     CDS             complement(32036..32659)
                     /locus_tag="PWT25_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020680.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32818..34215)
                     /locus_tag="PWT25_000572"
     CDS             complement(32818..34215)
                     /locus_tag="PWT25_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1020681.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34247..35218)
                     /locus_tag="PWT25_000573"
     CDS             complement(34247..35218)
                     /locus_tag="PWT25_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1020682.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35592..36017
                     /locus_tag="PWT25_000574"
     CDS             35592..36017
                     /locus_tag="PWT25_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020683.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36505..37206)
                     /locus_tag="PWT25_000575"
     CDS             complement(36505..37206)
                     /locus_tag="PWT25_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL1020684.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37222..37662)
                     /locus_tag="PWT25_000576"
     CDS             complement(37222..37662)
                     /locus_tag="PWT25_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL1020685.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37662..38237)
                     /locus_tag="PWT25_000577"
     CDS             complement(37662..38237)
                     /locus_tag="PWT25_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL1020686.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39085..39564)
                     /locus_tag="PWT25_000578"
                     /pseudo
     CDS             complement(39085..39564)
                     /locus_tag="PWT25_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39723..40424)
                     /locus_tag="PWT25_000579"
     CDS             complement(39723..40424)
                     /locus_tag="PWT25_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1020687.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40532..41941)
                     /locus_tag="PWT25_000580"
     CDS             complement(40532..41941)
                     /locus_tag="PWT25_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL1020688.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(41989..43854)
                     /locus_tag="PWT25_000581"
     CDS             complement(41989..43854)
                     /locus_tag="PWT25_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1020689.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(43975..44826)
                     /locus_tag="PWT25_000582"
     CDS             complement(43975..44826)
                     /locus_tag="PWT25_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1020690.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(45136..45849)
                     /locus_tag="PWT25_000583"
     CDS             complement(45136..45849)
                     /locus_tag="PWT25_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL1020691.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45917..46243)
                     /locus_tag="PWT25_000584"
     CDS             complement(45917..46243)
                     /locus_tag="PWT25_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL1020692.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46366..46593)
                     /locus_tag="PWT25_000585"
     CDS             complement(46366..46593)
                     /locus_tag="PWT25_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL1020693.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(46915..48168)
                     /locus_tag="PWT25_000586"
     CDS             complement(46915..48168)
                     /locus_tag="PWT25_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL1020694.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48229..48969
                     /locus_tag="PWT25_000587"
     CDS             48229..48969
                     /locus_tag="PWT25_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1020695.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49055..49813)
                     /locus_tag="PWT25_000588"
     CDS             complement(49055..49813)
                     /locus_tag="PWT25_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL1020696.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49810..50838)
                     /locus_tag="PWT25_000589"
                     /pseudo
     CDS             complement(49810..50838)
                     /locus_tag="PWT25_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(50858..54025)
                     /locus_tag="PWT25_000590"
     CDS             complement(50858..54025)
                     /locus_tag="PWT25_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020697.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54249..55133)
                     /locus_tag="PWT25_000591"
     CDS             complement(54249..55133)
                     /locus_tag="PWT25_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL1020698.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55228..55665)
                     /locus_tag="PWT25_000592"
     CDS             complement(55228..55665)
                     /locus_tag="PWT25_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020699.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(55882..56793)
                     /locus_tag="PWT25_000593"
     CDS             complement(55882..56793)
                     /locus_tag="PWT25_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL1020700.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(57250..57678)
                     /locus_tag="PWT25_000594"
     CDS             complement(57250..57678)
                     /locus_tag="PWT25_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020701.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(57706..58344)
                     /locus_tag="PWT25_000595"
     CDS             complement(57706..58344)
                     /locus_tag="PWT25_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020702.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(58348..60330)
                     /locus_tag="PWT25_000596"
     CDS             complement(58348..60330)
                     /locus_tag="PWT25_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL1020703.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            60510..61175
                     /locus_tag="PWT25_000597"
     CDS             60510..61175
                     /locus_tag="PWT25_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020704.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(63110..>64981)
                     /locus_tag="PWT25_000598"
     CDS             complement(63110..>64981)
                     /locus_tag="PWT25_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL1020705.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT        18900 a        13465 c        11010 g        21606 t
ORIGIN      
        1 actgtttttt ttaaatgatt aataaataac aacttctttc tatgaaattg caaagcattc
       61 atcttctttt ccgctaacga tatatccgtc agtgcttggt acactttact aaaaagtatg
      121 agattgccga tatctggata ggtgtgctat actacagaaa aagatataca atctgccacc
      181 gggtaggata aagaggatga tcattccatt aggtctttga agggatgatt ttcctctttt
      241 tgtgtatttt tttatagtta aggaggaatg taagtatggc atggatggaa ttaatcatag
      301 caggaatttt ggaagtattc tggtcaacga tgatgaaatg gagtgatggt ttttctaaaa
      361 tcaattattc tttttacaca gtgattggca tgattgctag tttttacttt ttgtcaaagg
      421 caatcaaaag tttgccgatg agtctctctt atcctatctg gacagggatt ggcgcagttg
      481 gttcggtcat aattggtgtg gtcttgtttc atgataagct gaatctttcc acttggttct
      541 ttgtggggat gctactcatc agtatcattg gcatcaagat tactagcggc cactagacac
      601 ttggaataag tactgatcag aaaaaataag cctttttatt taatattcaa tttttttatg
      661 aaaaaataag aaataagtcc aatgacctag cgacttctcc actcatcagg tgtatgcttg
      721 tactatctat agaaaaggag tgaatatttt tgaaaagtct ttcggtccaa gaattatcca
      781 agaactttgg tcacaaacaa gccctcagcg atgtcagttt tagcttatcc cctggcaaaa
      841 tcgttggatt agtaggtcct aatggcgctg gaaaaacaac gatcatgaaa gctattttag
      901 gactgatcca ttactcagat ggaacaatct gtattggtga aaatgagatt tctccctcta
      961 gtcataaagg gttggaacaa gtaggagcat tgatcgaata cccgggtatc tatccattct
     1021 taacaggcta tgatcatctg aagttattct ctgaaaccaa cgatgtatct gctatcgaca
     1081 ctatcgtgaa tcagctcaag atggaaaagt acattcacaa aaaagcgaaa tcctactcgt
     1141 tgggaatgaa acaaaaattg ggaattgctc ttgccttact gaaccatccg tctatcgtta
     1201 ttttagatga gccgatgaac ggtttggatc cacaagcaac aagagatgtg cgaaaaatca
     1261 ttaccgactt ggctactcag ggcacctctt tcttgatctc cagtcactta ctcagcgagc
     1321 ttgaaaaaat ggtcgatgag ctgatcctga tcaatcaagg aaagatcatc aaacaatgta
     1381 caatggctga attgaaccaa acagcgcaag atttcatcgt tatctctaca acaaatgatc
     1441 tagctgctcg aacgatcttg acagaagctg gatatacttt ggaagatcct gataaaatca
     1501 agcttttaaa agaaaacgaa gaattattag gaaccgtgat ccagctattg tctgcacaaa
     1561 atatccatgt gacagacgtc cagcatctga cgaatgattt ggaatcttct ctattggaaa
     1621 ttctggattc agtaaagggg gaagaagaaa aatgacattg cttgtaaaac aagaattatt
     1681 taaattgatc aagaaaaagt caactgctgt tttatctgtt ctattagttg ttttgctcat
     1741 aggaacagca cttctagcca aaaaatacac gacgatcatt gatccagttg aaatgactgc
     1801 tcagcttttt agtgccacat cttggattgt ctttatcatg attgcagctg caagcacgat
     1861 catttcgatg gaagcacaat atggtacatt gaaaaatctc ctttatcgaa agtattctcg
     1921 aggagaaata ttagtaagca aatggatcac attagttatt tattctgttt atttatacct
     1981 tttagctatt attgtcactg tgttgatgaa actgattctt ttcccttcga tttccttcac
     2041 ggagaaggtt tctactggtc aaacactgat ccaaagcttg ttcacttata ctttgggtag
     2101 ttatatcggt ttatggctga ttttaagttt agtgttgatg attgcttgct ttatcaatag
     2161 ttccggcgca tccatctcag caggaatcgt cttttatttt gcttcgtcaa tcatatctgg
     2221 tatccttatt gcactcatcc aaaaatggga gtggatcaaa tggaatccga tcagtatgtt
     2281 gaatctgcaa aaccaaattg gaaacgaaga gatcatgaag caattgactc atctttctac
     2341 taatcaaatg ctattcggta atttagctta catcgttttg ttcttagcat taggatatct
     2401 cgtatttaag cgaaaaaatg tatgatgact catcaaaaaa gaggctgtga aagagacgtt
     2461 cagccttatt gccgattggc tgtctcttaa acaccgttta ttctgaaaaa ggagctgtga
     2521 caacatttgt gtcacagctc ctttcctagt atgtttttga ataaacgaca ggctcacaac
     2581 ctctctttat acttctttta tacttctata cgtgaagcat cccagtggca aataaaacca
     2641 ttgcaatcac tccgaccatc gtcacaccta taagtaaaat cttatctaag tggatatgtt
     2701 gtacaggctc gttttgctcc ttacgtgctt tgaggtaaaa ataaatgcct ggcaagaaaa
     2761 gaacaagaac aagcattact tcattaaagg ctacgactag catagcccat acttgaaagg
     2821 cacttgctaa aagccctact aaaaattgga cccattcttt ccgctgataa gaaagtttca
     2881 tttggtacaa tcctaagaaa agccacgtga agaaaatagc tgctgtacca agtgtgtaag
     2941 caaagttata agcactgtca gtgaataata atgaaagtaa aaagacattc gtacataacg
     3001 tcgtcaatac gatagcataa gttggtgccc catttttgtt gagttttccc catgctttag
     3061 gcaaaagtcc gtcttcagcc atgtttccca ttgtttcaat cggtaagatc gtccatgaga
     3121 gccaaacacc aactaaagaa atgatcaaac cgccgttgat cacgactgct ccccatttac
     3181 caaccatcat ttcaaacaca tagcccatag caggctgcgc cttgatcgtt tgcagctgat
     3241 tcatcgtcat tgtcccatat ggaagcatgg aaaccaagac gtaaataata atcaagccga
     3301 atatccctag aatcgttgct ttcccaacat ctgattgttt ttttgctcgt gaacctacga
     3361 cagaggctcc ttcaatacca acaaataccc aaagcatcac catgatgcaa ctcaatactt
     3421 gactggaaaa cgggatcggc tgtccattcg cattcaatat aatgtcgcta gaaaagttat
     3481 caatgaattg ttggatcgaa aacgtgctga tagctactac aataaataaa aataatggca
     3541 ctaatttaca gaatgtgacg atagcattga cgaaactagc attttcgact ccccgattca
     3601 ctagccatgc atatccccat aaaaagactg tactcatcac gatcgatgcc agattttgcc
     3661 caccttcaaa tactgggaag aaatatccta atgcgctcat caacaatgtt gcaaaagcta
     3721 cgttgcctaa ccagcatgtg agccagtaac cccaaccgct gataaaacca gcgaaacggc
     3781 caaaaccttc ttctgcgtaa ctaaagatcc cgccttttaa atctggacgt tttttattga
     3841 tattattgat cgacaaggag agcatcaaca ttgctgttcc tacgagtacc caactgaaaa
     3901 tagctgggcc gccagccgat ccggagatgt ccgtcataat accaaatacg ccacctccaa
     3961 tcgctgaacc aattactaag gcagcaagtg gaattaaacc aattcctttt ttctgttgct
     4021 ctgtatccat gtaagcatcc tccaaaataa aaccgctttt atcaaaaagt ataacaaacc
     4081 ttttttattt ttacttaatt aataaaaact ttaatagaaa tataattcta ccggcttttt
     4141 tctcattata caagcaaagc gcctagtccc tttccaaatt acaccaccaa aaaacttaaa
     4201 aaagatgcta tactaaaagg aaagaattgg aggatgatcc atgaacattt tatatatatc
     4261 gatttcagga aatacgcgtg cctttgccaa acatctagca gaatatgctg aaaaaatgca
     4321 tgcagaagat cctgtcaatc ctgaagttac tttaaaagaa atccatgaaa atagtgattt
     4381 tacgaaagaa acagaaccat tttttacttt cgttcctact tatttagacg gcggaaatgg
     4441 tttagataac ggtgatacag aaatcttgac cgaaacgatg cgtgaatatc tggaacatga
     4501 agacaatcat aaactttgtc ttggtgtagt cggtagcgga aataaaaact tcaataatca
     4561 gtattgtcta acagccaaac aatatgcaca aacatttggc tttcctttct tggcagatta
     4621 tgaattacgg ggaacaccat ctgatgtgac acgaatttat cagattttag cagaaaacaa
     4681 taaataataa aaaagaagaa gctgaaaaat agatttcagt ttcttctttc gtttctattg
     4741 tttctatcat attaagcttt gacatccatc acaggtgctt ttgcttttgc tttacctgtt
     4801 ttttccaaga caaagttttt tacacgatcc atattggtta ccaagacgat catggatgtt
     4861 tctttgcctg ctgctttgat cgcttctaag tcagcttctg ctacaagcgt atcaggtgtc
     4921 acttgctgcc cttctgttac tttcacatca aatggcgtac cgcctaagtc aactgtgttg
     4981 attcccatat gaagcaagat ttctaaacca tttggcgttg tgatcccaat agcgtgtttc
     5041 gttgggaaga cagagctgac taccccagta actggagaat agatatgtcc gttttctggt
     5101 tctacaaagt agccttcacc catcatcttt tgtgcaaaaa ctggatcact tgctttatct
     5161 aaagaaacaa ctgttccgtc agcaactgat gagaggatag tcacactttc agctgaagtt
     5221 tgaggtacta ctttttcagg cacttcattt actgtggctg tttcagtcgc taattcttct
     5281 ttttctaaac ggtctttagg aataccgaag aagaatgtag caacgaagcc accagcataa
     5341 gctgccaata atccaaggat gtagcctaac catttaccgt cagagatcaa tgggatcaaa
     5401 gcggctcctg aaggtccaat agcgatagcc ccgacatttc cgatcattcc gatcactgcc
     5461 ccgccgatac ctccaccgat acaagcagta ataaatgggc gtcctagagg taatgttact
     5521 ccgtaaatca atggttcgcc gattcctagg atcccgactg gtaaagctcc tttgatcatt
     5581 tctgttaatt ctttatcttt tttgcagcga atccataatg ccaatgctgc acccacttgt
     5641 cctgcaccag ccatcgcaag aattggaagc aatagcgtac gaccagtttc atcaatcatt
     5701 tgcacatgga ttggagtcaa aatttgatgc agtccgaaca taaccatcgg caagaataat
     5761 gtgcctagaa cgaagccggc aaatgctcca ccaacgccaa gtacccagtt gatcccaccg
     5821 accatgccat cagaaatgaa acctgctaaa ggcatgatga agaaaatttc aatcagaccg
     5881 atggcaatca atgaaagagt tggtgtaacg atgatatcta ccgcatcagg tactacttta
     5941 tgcagttttt tctcaacgat tgacaatagc caaacagcga agatgacacc taggatccca
     6001 ccttgtccag ctgcaagagc ttcaccagta aataagtttt tcagtggtgc ttccggattc
     6061 atccctgtca gcatcacaac ggccccgata actccaccaa gagttggtgt tgcaccaaaa
     6121 acttgtgcag cattgatacc tgtatagata actaggtagc taaacatccc gtttttcaag
     6181 atgttcatca catcgatata ttgttgccat gttgctgcat caagtgttcc ggctgtgact
     6241 aagtttgcta aaactgcggc aatccctgcc acgatcccag taccgacaaa tgcaggaatc
     6301 attggcacaa agatattaga aatgtctttt aataccgatt tgattttaga tggtttttgt
     6361 ttcgattttt gtgccgcttt catttcttgt gcccgttcgt ttacacggtc ttttccagat
     6421 gctgatgaat ttgctgtagc tgtacttaca gaagaaagtt tttctcctaa tttcacgcct
     6481 gccaaatcaa ccatttgctg tgctactttg ttcactttcc caggtccgat gatgacttga
     6541 agctgttcat cttctactac accaagcact cctggaatcg cttttaattc agagacattt
     6601 acaagatcat cattgatgat cttcatacgc acacgagtca tgcagtggac aatgctttct
     6661 acattgccca tgccgcctgc ccctttataa atttgttttg ccagttcttc caaagtcagt
     6721 tcgttttttg ccatggtctt ttccccctca taaggtccct tttacaaatc cgtttgcttc
     6781 agctaatttt gcttgagctt cctctttatc actatttgtc aaaatcatga cgatagccag
     6841 cttaacattt tgatctgctt cttcaaagta cttttcagct gtttcgtaat cagcttctgt
     6901 cgcttgcatg atgattcgtt ttgagcgttc gattagtttt tcatttgttg gtttgacatc
     6961 gaccatcaag ttattgaata ctttacctat accaatcata ctgatagtag aaatcatatt
     7021 caagataagt ttttgtgctg ttcctgactt caatcgagta gagcctgtca aaaattctgg
     7081 acctgcatcg acttcgattg ggaaatccgc gtgtttactg atttcggctt gtttattgca
     7141 agaaatcgtt cctgtgacag caccgatcga ttttgcgtaa tctaatccac cgatcacata
     7201 tggtgtacga ccgctagctg cgataccaat aactatatca ttttttgtca aattcagctt
     7261 gattagatct tcttgcccta gctcataaga atcttcggcc ccttcgacag ctacagtcat
     7321 tgcttcttct ccaccagcaa ttagacctat gaccatttcc ggttctacac cgaatgtcgg
     7381 tacgcattca gcagcatcta acacacctag tcttccactt gttccggctc ccatataaat
     7441 cagacgtcca ccttttttaa aggactcaat cgtgttagca atcactggtt cgatacttgt
     7501 cagttctttt tcaaccgcag cggccacttt ctgatcttct ttgttcatta gttgaacggc
     7561 ttgagaaata ctcatctcat ctaatccaaa agtttcttgg tttctgcgtt ccgttgttag
     7621 gttctctaaa ttgattttgc tcatttttat cttccttctt ttcttataaa ttgatagttc
     7681 ataccagctt tgatttggtt gatcaatggt agctcttctt tgataacttt agctactaca
     7741 ttcacttttt catccgctgg caaatcatat ttggttaatt ggatctcacc catatagcgc
     7801 aaatactttt cgttatctaa agtaatagac cctttcatac gcgtagctgt atttcttgct
     7861 gggataggcg gaatctctcg aaaacgtgca tctacgcttc gtatgacatc tcttgcatcg
     7921 tcttgccgat tcgtatgctt ccctaatata tattcgtaaa attcttcatc tacaggttcc
     7981 gtatggagca agactgtctg ttctttttgg aaagaagcaa attgctcctg aacgttttct
     8041 gacagacctg catctccgat gtaaacaata tccgtgttac ttgctaatag atctagagcg
     8101 cctgcaagag gatggatccc tcgatgttct tccaaagttg gcaatccttg atatagtggg
     8161 cctctcaact ctgtatctcc tggtacaaaa ccttggacaa ggagaccttg aattttgagc
     8221 cattgatttt ttttctgata ccattctttg tccagccctg tttcgggacg tggataataa
     8281 ttatgccaag cttcaatctg tgagaaatca gcttctgctt ctttcaattc atcgatatct
     8341 ttaggcgtga tcgtgctagc attcaaactg attttcaatt gatgtgacca gtcggcaatc
     8401 tgctgattgg aaatatgata atccattctt aaacctgtta caccaattgc tttcaaaggc
     8461 gttagatctt ctaatgaaaa acccgctcgt tccaatgcct ctcctgaaat atcgaccatc
     8521 aattctagtt tattcttttt cgcttcgctt cctagttcga tcaatcgctt ttgatatgcc
     8581 gaaacatcat cttctggtat atgcatggaa gtgaaaatac cgacaaagcc attggatgcc
     8641 atttggcgga tatactgttt cttctcttct gacaaagaat cgttcataaa tattgaaaag
     8701 ccaaacatat tatccctctt tttctgtatc cgtcttcatt tctatgacta gagtatatat
     8761 ctaaagaaac aaaatttcaa tacttttatt aaaataatta aatttcattt ctaaaggttg
     8821 agtttatatt atcaaaagaa ttgtcataac ctaaaaaagg cactgctaag caaaagcagt
     8881 gccttgaaat attttattta aataacacaa aaacataatt actcataaga aaccaattct
     8941 gtattatcta ccatgaatgt aaagaacccc tcattaggca tcggatgaaa ataagcattg
     9001 atcactctta tcaaggcatt atggcaaact aacagataga cattttcttc gtcttcgatg
     9061 cattctttca ataaaggaac gatccgtgca tacacatcta agactgactc cccatttggg
     9121 aatcgtacag cgaacgctaa acgtgctttt tgaaagttct catctttgct tggcagtcca
     9181 tcataatccc caaaatccat ttctaccaat cgttcatcaa cggtcagcgg caagctgaga
     9241 cgatcagcaa ctgcttgtgc tgtatctctt gctcgttgaa gaggggaatg gatgattttc
     9301 gtgattggaa caggcaaatc tactaccttt tctgctagtt tctgtgcctg catctgtcct
     9361 ttttcagtca gcggcacatc tgcccgtccg caaactaatc cttgtgcgtt ccaagttgtt
     9421 tctccgtgtc tcgttacata aagcatgtga ttctctcctt cgttggcaaa gttcccctta
     9481 accatacctt gttgttcttg agattacaag ggttagattc tctcaagaga aataatctca
     9541 gtagatactt tatccaggaa ggtttgatca tgttccacta gtaacatggc aggtttcacg
     9601 ttcaagatca gttgttctaa ttgttcttga ttgaagacat ccaaataatt caatggttca
     9661 tcccatgtat atagttcagc tggctgtgat aaagatttag ccaattccac ttttttccgt
     9721 tggcccatac tcatctgctc gatcttgtta tgaaaaacat ctctttccat cccaagcttt
     9781 cggaggttgt tcaaaaatgc ttggtagtct acttggtttt tctctgcaaa ttccgccaac
     9841 gttcctcgat tgtcttcata attttggctt gcataactaa tgctcagatg ttttggctgg
     9901 tatttttctc ctataacctt gccgttgaat gcccccagaa gataatggat aatggatgac
     9961 tttcctgcac cgttaggacc tgaaatcgcc acccgctgat gaggctcgat tgtaaaatga
    10021 attggctcga ataacagatt ttcataccct aattgaagat cttctacgct taaaagtcgc
    10081 ttatggtgag acgcttggct attcatcgtc aaagaatcga tatattcgat atcttttaat
    10141 agtttttctt tctcgctgat ctgggtctcc atccgattca cgattgcttt ggatcgtttc
    10201 atcgtccgtg cagcatcagc acccactgct cctttattca ctcgtctaga ttcagtatcg
    10261 atgaatccga cttgtttctt tgttttatct ccttctcggg aacgagacca ttcggctttt
    10321 tcagctgctg ttttctttag cctactgact tcttttttca atttttcatt ttgagccatt
    10381 tcaaattcat cacgaagttt tttctgttct tcatagatag agaaattccc ttgataaagt
    10441 tccagttgac ttttttcgat tgctaaaaca tggtccacta cttcatcgat aaatccccgg
    10501 tcatggctga ccacgatgaa gccttgtttt ttctttttca aataagccgc tacttgtttt
    10561 ctaccagaga tatccaaatg attcgttggt tcatcgatta acgggaaatg agtgtcatcc
    10621 acaaataaaa gtgccagtag gacttttgtc ttctctcccc ccgatagtgt gctgaattct
    10681 ctccataaga tttcaggatc tgtttgcatc aattggagct ctctttcgat ttcccatatc
    10741 tcaaaatccg taatatcatt taacacgtaa taggttaaac gttctttatc ttttgtctgt
    10801 tgcgggaaat aggcaaattc ttgctgatgg attacttgcc cttgataagg tagtttgttt
    10861 tgtagaatat tcagtaaagt tgtctttcct cgaccgtttc gtccgataag tcctagtttc
    10921 cattgtgtgt caaaatttag atttgcttgt tcaaataata atgtgccttg gctgtcgtag
    10981 ccgaatgtca gattttttat ttcgatttta gacattttcc atcacctcgt aaaaaaagac
    11041 cagtcctatt ttcagaactg gctaaagagg atgcaagggt tggatacaca caaaaagacc
    11101 catacatcga aaaaaccaag ccaatcccga aaatctgcac actgcttcct tgtctccaca
    11161 aggtacagtc tgaatgcagc tttcatcaat cgacttaatt tttcaatgca cggatccctc
    11221 actttgtcct atatttgaaa ttacgatagc ataaagaaaa tgattttgtc aactacttcg
    11281 ggaggatggt gaaaaatctg ctaagtgtct gtcctaagat ttttgttttt tctaatgttt
    11341 gcctgtgttc cattgcttct tctaaaccta ttggacccaa atgaatactt aagacaattg
    11401 ggagaaaatc agaaagtcct tctagctctt ggtctctact tccgcaaata gcgattattg
    11461 gttttcgata tttttttgca agcactgcta caccgtatgg aacttttccg tggatcgttt
    11521 gctgatcgat ccgcccttct cctgtaatga tgacatctgc ttgttcgatc tttttttcca
    11581 atccagtgat ttggctgact aaatcgaaac cagaaaccat cttcgctccc aacaaaaaaa
    11641 gggctccacc aataccgcct gctgctccac ttcctggaat atcatctaaa tccacctccc
    11701 agaaatcttt tatttgctga gcaatctttc ctgcttgttt atctaagaaa gctagcgtcg
    11761 tatctattcc tcctttttgt cttccaaatt gccaagcagc tccttgttga cctgtatatg
    11821 gattggtaac atctgcagcg ataatcaatt ttactttaga aacaagaacg tttcttcgat
    11881 caaaatgaag ttttttagta gataataaag gatttccttc tgtgtaccct tctaaagaag
    11941 cacccaagga ttggagtaat cctagaccac catcgctaca cccgcttcct cctaaagtga
    12001 caataatttg ttctacttct tgaagcagtg catctttaat aagttctcct aaggcataac
    12061 tggaagcaag tcggatagtt tgatccgttg gtttatcaag tagatctaac cccaatgctt
    12121 gtgcagattc gatgactgcg gtgttttttc cttgccaagt tgtcaataaa tagctgattt
    12181 ttcgctgacg gaataagaga tccatcccat caatggtacg ccatgttccc ctgagcgcgt
    12241 aatagattgc ttctgcagta ttttctccgc catctgcaat cggcacattg atttttcttc
    12301 ctttacgatc ttcccagcca tttagaactg cctgatttag ttcaaatgaa gaagcacttc
    12361 ctttaaatga atcaattgcc gccaaaatat ccatctgtat ccctcacttt cttcttaagt
    12421 aagacacttt ttttcatttt tttcaaacaa aacaatgcta ttttcttcca tttttctata
    12481 tactaaaagg gaactcattg aataaaggag agaaaaaaca tgaaaatcgc aatagctggg
    12541 gccggagcaa tgggcagccg aatcggattg atgctacatc aaagcggaaa tgaggtcctc
    12601 ttgatcgatc gttggcctgc tcacattgaa gccattcgta caaatgggtt gattgctgac
    12661 tttaacggta aagaagtggt cgcaaaactg ccaatctatt caccagaaga aatcatcgaa
    12721 agcaacgaac acgtggactt gatcgtagca ttaacaaaag ccaatcaatt agatgatatg
    12781 ttttgttcga tccaatccat tattactgac aatacttatg ttttgtgttt actaaatgga
    12841 ttaggtcatg aagatgtact ggaaaaatat gtgccaaaga aaaatatctt atttgggatc
    12901 acgatgtgga cagctggtct agctggtccc ggaaaagtca cattgttagg tgatggagaa
    12961 atcgaactag agaacttgga accagaaggc gaagcattta caaaaaaagt tgtagaagtc
    13021 tttcaagaag caaatttaaa tccgatctat agccataacg ttcgttattc catttggcga
    13081 aaagcttgtg taaatggaac attgaatggt ttatgcacga ttttggattg taatattgct
    13141 gaactaggcg cgcaaaaagc ggcagagagc atggttcgaa caatcgtatc tgaatttgct
    13201 tctatcgcag caaaagaagg aatcatacta gatcaagaag aagtgtacca acatattgct
    13261 tctacttatg atcctgacaa catcgggttg cattacccat ctatgtacca agacctgatc
    13321 aaaaaccatc gtttgactga aatcgactac atcaatggtg ccatttggcg aaaaggacaa
    13381 aaatatgata ttgctacacc atattgtgcc tttttgaccc aattagtcca tgcaaaagaa
    13441 ggaatccttg gcgcagaata aaaggctctc tgatgagaat cagctgaatc agcagctggt
    13501 tctctttttt taagcccaat aaaagatcaa aaagaaaaca caactctcat gatgccataa
    13561 gaattgtgct tgtttgtttc agtacataat ctcttcaaaa tgtaggtttt ttatttgttt
    13621 tcaacagagg agaggtcata ttcgtattgc tcataaatag tggaatcgtt agtgataaat
    13681 cgttttgccc aattttctgc atcataaaat tccaatagct ctgataggat tggcttaaat
    13741 ggtgtatgca gaaattccgg taattcatct aaatcacgtt ctcgaagcaa aaaaatattt
    13801 tcaggacgat aaaaatgata atgagcaatc gcttgattat ccgtcactag tttcttttga
    13861 aaatacatgg attcaaatgt ccgcaaagtc actccttgct gaccctcccg caacagttcc
    13921 agaatcccgc gcgattcccc tgtttttttc aggtactctt cataagtaag atatccaggt
    13981 tgttcattcc ctcgatgtgg aagaatgaaa tagttggtcg taacatctct ttttataaat
    14041 tcctgcttat agctttctgc tcgttctttt ctcccattat tcgttgcacc aaaaaataga
    14101 tccgtctttg gttcgctaga agaaaaattc atatgtttcg aataaaatga actattatgt
    14161 ttcagcccaa gttcttttgc ttccaaaaaa tcaaaatgat aaatctcatc gattgccggt
    14221 tcatcttgtg cctgcttcag catcgcctcg tgttctgctt tgaagtgatt ccaaaaaaat
    14281 aacacaattt ttgcattggt aaatgggcgc acgcgtcgag ccatctccat atcaaatcca
    14341 taatctaaaa aaacgatcag ctgataattt gccaactgtt gtctagctga tttaatgaat
    14401 tccgactgta gtaaatcttt tccggaccaa acatctaaag agcggatgga aggcaaaaaa
    14461 taaagtgatt tattttttgc aactagcagt acgtcatttt tgttcatctt ttccgcctca
    14521 ctttctctgt cactttttcc tatgatagcc tgtttaaata gtaaaaagcc agtaaaacgt
    14581 ttcctatatt tgtatgaaaa tttaacattt gtatgaaaat atgatgaaat aaatcattct
    14641 tctattaaaa ttcgcctaaa agttttctga atagttgtaa ttgtctgaaa atttgttatc
    14701 attagtcatt gttaaatatt gctatttatt cttaatataa attttgattg tgaatttaaa
    14761 agaaagaggt catttatgga agataaaaaa ttgaaaaaac aacttagctc cagacatatc
    14821 accatgttag ctttaggtgg agcaatcggt gcaggtctat ttaaaggaag tggcgaagcg
    14881 attgggatcg ccggcccttc tgtattgatt gcttttttga ttggtgggct gattttatat
    14941 atcgtgatga aaggtctggg aaaaatcgtt ttaagcggtg gcgacacgca tcacggatta
    15001 tctggtttga tacgccctta tctaggtgct cattctgctg attttactga ttgggtctac
    15061 tggtctatgt ggattatcaa tatcatcgct gaagctgttg cagctgcttc ctttttgcag
    15121 ttgtggttcc cgcacattcc agcctgggta tttgttttta tgttggctgt tttaactacg
    15181 atcatcaatc tttattcggt acggttattc gcagaaacag aatattggct ggcttttgct
    15241 aaaatcagtg tcattatctt actgatcatt tttgctactt atcttgtcgg tcagcaaatg
    15301 ttgggcacag gcgttttccc tacccttcag caattgactg accatggtgg gtttactcct
    15361 catggtatga agggaatcat cagttctttg cttgtagtta tctattctta cggcggttct
    15421 gaactgattg ctatcacggt aagcgaagca gatgatccta aacgtgcgat tcctaaagca
    15481 atcaaaggtg tcatgggaag aatcatttcc ttttacatca ttcctttgtt tttgcttttg
    15541 attctttttc cttggaatac tttagcaggc acgaatgtca gtccttttgt gatggtattt
    15601 gaaaaaatga atatcccatt tgcagcggat atcgtcaatt ttgtgattgt ccttgctttg
    15661 ttctcatcta tcaattcggg tgtgtatgca tcttcaagaa ttttgttctt ccgtttgaag
    15721 gatcgaaaag gttcctctag aaaactagct gttttaaaca agcatcaagt accgcaacgt
    15781 gctgttttat tttgtaccag tgttctttat cttggtgtag ctctttctta ttttgtcggt
    15841 gacaaattat ttggctatct ggcaggttcc ctttcgtata cagtattatt gatctggatc
    15901 attatcagtg cggcatcctt tgtactggca ctaagaaaag gaacattatg ggataaagga
    15961 atcagctttt ttgcattagc tgtcttagga ttgattttcc ttggtatcct gttcacgaac
    16021 tccattgggg taactgtatt gactggcctg ctttacctgt tcatttattt cagttatcaa
    16081 aaaaagaatg atgcgttcgt attgacgaat gaatgaaatc gatagaaaat tttattttat
    16141 ttatgtggaa tagatggagc tattgaagaa aggagttgtg acaaaagtct tgttgcaact
    16201 cctttttccg aataaacagt ctcatgacct cttggtaaac ggtgttcaaa aaccctctgt
    16261 gcaaaataat tgattgttct ccatgctcat gacatgatag aagaaaggaa tgaaaaaaag
    16321 gagtttgttt taaatgaaaa agtattggaa gtttttgttg atgatcggtg tttcaacggt
    16381 agttatgttt ggtatgatgt acttgaatgt ctatgcgctc gatcatatct tcttcagtcg
    16441 tacacgagta ttcatggcgt tgatgatggg tgcagtcatg gcagtcatca tgctgttgtt
    16501 catgtggaaa atgtatgaaa acaaaaagat aaatgttatg atcttagccg ctagtcttct
    16561 tgtttttgcc ggttccttgt ttatggtacg aagccaaact actgtcaacg acacagcttg
    16621 gatgaaggcc atgattcctc atcattctat cgctatcctg gcaagtaaac gagctgattt
    16681 aaaagatcct gaagtaaaaa aattagcgga agaaatcatt caggcacaag aagaagagat
    16741 cgacaaaatg aaaaaaatga tcaaagaaat ggaatgacca ttatccgtcc aaataaaaaa
    16801 agaggatgtt ccagaagtta tcagcttcaa gaaataagaa gaagctactt ctggaacgcc
    16861 gtgaataagg ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa
    16921 atagcttctc ctatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca
    16981 ccgtttatta agaggttgta aaatcagcgt tctgacctga gtattagaga gacaaatgga
    17041 aaaatagttc ctcatatttt gccatttttt gagctaatac cgagggtcag ctgattgaac
    17101 accgtttatc gagaggttgt gaaaaagtcg cttagtttcg cgtattaaga aaaattttgt
    17161 gaaatggctt tccacatttt tgtataaatt tggcttaata ccgagaaact ggcttttgaa
    17221 taccgtttat tcgtaaaagg gattgtgaca aaagtcttgt cacaatccct ttttatgttt
    17281 ttattgttct aattgcgctg tcttgcttac tgcttcttgc ggtttcacaa atttttttga
    17341 gattccccaa cctgtgaagg cactgaccat cgaaaacaca ggagaaagca aactgaaaaa
    17401 tacgaatggc aagtaagtga gtgtaggaat accaaatgta cttgctacga aacttccggc
    17461 aattccccaa ggcactaagt agttgattac tgttccacca tcttctagcg ttctacctaa
    17521 tacagttgga tcaaatcctg ctttttcata agtttcttta aatgcattac caggcagaat
    17581 aacagatagg aattgttcac caataaaaat atttactccg atacttgtca tcaatgttgt
    17641 aaaaatcaaa tttggtgtgt tcgtcatctt tcttgatact cgttccatca cggttgagat
    17701 caagccaaat tcgatcaaca atccacctaa tgacaaagtt agaataatca atgacacagt
    17761 aggcatcata ctttcgattc ctccgcggct aagcagtgaa tcgatttgtt ggtttccagt
    17821 atttgaaaca aaccctgatt cgatgacccc agccaacttc gttaaggaag tttgtgggtt
    17881 ttgaataaaa atcattccta ctgctacagc gatattcaac aaaatcgtta aaattgccgg
    17941 cacctttttc catgcacaca gtaacattaa aaataaagga atcagtgacc agattgaaat
    18001 agtaaagttt gcttctagaa catcagtcac ttcacgaata gatgaaagac ttgcggcttt
    18061 acttgtgtgt cctaaaatca tgaataaaaa gaaagaaaca acaaatgccg ggacagttga
    18121 ccacatcata tgcttgatgt gtttgaacaa atcggcatca acaatagctg ctgctagatt
    18181 tgttgattcg gataatggag acatcttatc tccaaaaacg gcacctgaaa caatagctcc
    18241 aacaaccagc gctggattta agcctagtgt tgtcccaata ccgaaaaatg caatcccaat
    18301 cgttgacata acagtaaagg cactccctac agcgctacct acgattgcac acacaacaaa
    18361 tacagaaggg acaaaccact ggacactgat caatttgaac ccaatgacca ttagagttgg
    18421 aatgatccct gcttgtatcc atacagcaat caatgaaccg actaaaataa aaataaagat
    18481 tgggatgatt cctggtttga tgccattgat gattccttcg tgcagccaat ctgtcgggta
    18541 tctttttatc actgcataca acatgaccat tccgatcgct gctaagatcg tgatattagg
    18601 tgagaccttt aatccgacaa cgccaatcgc aatgcctaaa acaataacca ttaatacaag
    18661 tgttgcttct aaaaaacttg gtgttccttt ttttcccatg ataatctctc cttttttagt
    18721 tgttcttatg ttctaaaaaa gttaagatat cggattactc cgccgcaatt catccgcgat
    18781 tcctccttta taaaaataaa aagacctgtt ttaaactaag gacgaatgac tccgcggtac
    18841 caccttaatt aaaaacagat ctgtttttca ctttcaaact tttattcaaa tgtatggact
    18901 ttccatgtaa ttcgtcaatt aggcttgatc ggttcacagc gaccaccgac tttctgaaca
    18961 aaacacctaa ccgctactgc gtgaaaagtt gacttgtacg aagtttacct tccataagat
    19021 catttgtcaa tgaaaaagaa aatatttttt catttttctg aaaattattt ttataacttt
    19081 cacacacaag tttatttttt actagaaagt agcgccctct tgtttttttc ttgctactat
    19141 ttcttgatgt tcttgcttga ttctttctaa tagctttggc tctttcaaca gatcaagagc
    19201 tgtattggcg agtagttctg ctgcaatcgc aatagaagca aggcctttgt cacttttagc
    19261 agcagctttg aattcttctg tgtgtccggc aatatatgtc tcagagattg ctaccgtagg
    19321 ctggattgtt ggtatgactt ggctgacatt tcctacatca gaagaaccga gacttgttct
    19381 ttgttccgtt tctatttctt catctggaac acctgcttct tttgcatgct ggaagaataa
    19441 gtcatcaaaa gaaggagtaa cgatgacatc atcaactgca ttttggaaca atccaaactc
    19501 aaaagtcgtg cccgtactta tcgcagcacc tcgtacgata ttttccactt tttcatatac
    19561 tttatctaat gtttgccgat ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg
    19621 aatgacgttg gcagctactc ctccgtctgt aatgatccca tggatattga catcttttgg
    19681 caattgcaag cgtagagcgt tgattccatt aaacacttgg atcaatgctt ccaaagcatt
    19741 tactcctttt tcaggtgctg ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac
    19801 tggatcattc gctaaagatt ctgtcgtttt cccataacga aaagctggat ggacgcataa
    19861 cgctacatcg acatcctcaa aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt
    19921 ctcgccccct tcttcccctg gggtgccata tacacggatt tctccaccag tttcatcaat
    19981 gaattgcttc actatgctgg cggctaatac ggagtaagta ccaaacaaat tatgcccgca
    20041 tgcatgacca atacctggta aagcatcgaa ttctgccaaa aaggcaattg tgggaccggg
    20101 tttattggaa atataacgtg catcaaaggc tgttcggtgt ccagctactt ctttttctac
    20161 ccaaaacctt tctttttcta attgtttaat caacgctgct gaggaataaa actcatagtt
    20221 acttacttca ggatgttcgt ggatatctag agcaagtgct tgatattctg gtaaacgata
    20281 ttggattaga tccttcaatt ggtttgttga gattgttgtt gtcataaaaa attcctccta
    20341 tgctagtcat ttttcttatt ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt
    20401 tgatgttttc tcgattactt tgactgtgtc ttctgtttga taagcgtcta ctattttttg
    20461 atacgtttta ttgttttcat cttcttttct tgcaacgatg atattcacat aaggacgtgc
    20521 atggtcatcc actggttcta agaagatggc atcagaagca ggattaaatc cagcatctac
    20581 agctacaccg ctattgatca ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga
    20641 agcatctaat tcagaaatgt ctagattttt tctattctcg gtaatatcag aaacagtggg
    20701 cgcttgtcct tttgccttat ccactttgat caatccagca gtttgcaata acagaagagc
    20761 tctgccgcca tttgtaacat cattagggat tgctactttt gctccgtctt tgatatcagc
    20821 tacatccttc aaagtgttag aataaattcc cagcggtgca ttgacagtat ttccgatcga
    20881 tactaaatca gttccgtgtt ctttattgta attgtctaaa aagaattgat gttggaaaga
    20941 attcaaatca atcgatccat ctgctaaagc cacatttggt tgattgtaat ctgtaaattt
    21001 cacgtattcc aaattaatgt tctccttttt caaacggtct ttcacgttgt cccatacatc
    21061 tgtatcttct ccaataatcc ctagttttac gatagtttct tttttgtcag ttgtatctgc
    21121 ttttccttgg ctacaccctg ccaaccaaat aattgctaca aatgtaatga ctacaaaacg
    21181 aataattttt ttcatttttt ctcctccact ttcaatgtgt aattttttta atcagttgat
    21241 tactgataaa ttgacttaaa aaaactaaga ttagaatcaa taatgtagcc acaatcgtta
    21301 catctgtttg gaatcgatta taccctctcg atatagcaag attccctaat ccgcctgcac
    21361 ctactgctcc agccatggta gtcagtccga tcacgttgat gatagtcaac gccgagaccc
    21421 gaatgatacc aggcaaacct tctaacaaat acacacgtaa aatgataccg attggactgg
    21481 ttcccatcgc ttctgccgct tcgatcaccc caggatcaac ttctagcagt gcgttctcga
    21541 tttgacgagc aaaaaacgga acaactccaa aaatcaatgg cactaaagct gctttcgcac
    21601 caatcgtcgt tccaactaag aaacgagtag tcaatgctaa aagagaaagc aagataatga
    21661 aagggatgga acgaatgata ttgatcagct tatcaagtat ttcaaatagc agacggttag
    21721 ctagtaatcc attagggcgt gtcactacta acgtcacacc taagatgatt ccaatcatcc
    21781 cgccaatgaa tgctgtccaa aaagacatat atagtgtttg cttcgtcgct tcgacaaatt
    21841 catccggaat caaataaaca ttgggcaaat atgttttaaa gaaatcagtc attttatctt
    21901 cctccttcga tcactttgat acgactatct aaatcgtttt tttgtaaaat atttattttt
    21961 acaccagatt tgatcaaata atccaatgct tttttccgct gtttggtttc tccagctaaa
    22021 gtaacgatca aactccctaa cggcgtttct tgtaataact ctacattgcc atataaaata
    22081 ttcgctgtta cttgatattt gctgtacaga tgagcgatta gaggttcgtt cgtctgattt
    22141 ccaatataac ttaattccac cagccattta ttggcgatct gatcggcaaa agcagaatgg
    22201 gcaatgatcg tttctaatgc ttggtcaaga tgagtagctg ttcgaataaa atctttggtc
    22261 aattcttctt caggattgct gaagatctgg atgctgctac ctttttcaac gattcttcca
    22321 tcttccataa ccgctacctt gttgcagatt tctttgacaa cttgcatctc atgagtgatc
    22381 aggacgattg ttaaacctaa ctggcgattc aattttttta acaatgcaag gatctgaagg
    22441 gtcgttttcg gatcaagtgc actggttgct tcatcacata ataatacctt aggttcattc
    22501 gccaaagctc gtgcgattgc tactcgttgt ttttgtcccc ccgacagttg tttaggataa
    22561 gcagatgctt tttcttccaa accgacaagt tccagtagtt cattcacttt ttgacgacgt
    22621 tcctgtttcg attttccaga atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg
    22681 ctttccatta gattgaaatg ctgaaagatc ataccgatgg cctttctttc ttttcgcaat
    22741 tctttgggag agagctgact cagattcgtc tgattgacaa tgacttctcc tgctgttggt
    22801 ttctgcagca aattcatcac acgaactaac gtacttttcc ctgcacctga gtaaccgacg
    22861 attccataaa catcgccttt ttcaatcgtt aaatctacag aatctactgc ctgtatctct
    22921 ttttcttttt gtttgaaaca tacagaaaca tctcgcaatt caatcattaa aattcctcct
    22981 tatctaaaaa aagcgtcctc aagaaaatga aaatgtcatt ttcttgagga cgcttttttg
    23041 cgtgttacca cctcatgttc aagctgtgtt cacacacaac tcctcttcaa gtacgatcat
    23101 actctagcaa gatatcggtc gcagccgtca tcgccttaca tctatgctcg ggatgaattg
    23161 ctcgatggcc attttccaat agtcgttcct tgcttcttct cagctaccga agctctctgt
    23221 caaaggttct tctatcgtac ttaccatttc aatgcaaata atattcaaaa aaaaagtcct
    23281 tttactaaca taaactatgt cagtaaaagg acgacgtatc gcggtaccac cttttttcgg
    23341 aagtttcctt ccctcaagcc caacatctca aaaagagaga ttggcgttcg ttttaacggt
    23401 cgaacagttc cgaatttgcc tacttatcga caaattattc tccaaggcca ttttcagctt
    23461 ttcttacttg actcttctca ccagccagag cctctctgca aagtttccaa acttactttc
    23521 cttttcaacg aaatcgatat tttgttgttg ggattaatct acatgaggaa aactgaaaag
    23581 tcaagtacga aattcaaatt attttttcta tatttatttt tctgtttttt tatttaacat
    23641 ttcaataaca gttcgaagat cttttccttc cgcaagaagt ttcaattttt ccttttcttc
    23701 gacggtataa tttcggacaa aactggaatc aggtatcaaa acaacgccta atcctgctcg
    23761 tgaagcagct gttgctccta ctaaagaatc ttccactgca aaggctgaac tagcttctaa
    23821 tcctgttcgt tccaaagctg tcagataagg aatcggtgaa ggcttgttct ccgacacttc
    23881 gtctccgaat gtaaacgtgt ccaagtaatc agctaattta aagtgatcca agatatctgt
    23941 tgctcttttc gtaacagtgg tcgtagcaat gcctagtttc atatgacgtt cttttaagtc
    24001 tgccaatatt tcttttgcat atggttttaa gtggatggct ccgcttgtca gctgttgata
    24061 aaaatattct tcacgcttcg ctcggacttc ctgtactttt tcagggctac ctgctgcttt
    24121 cactaaaata tcaaaacttt gtttccagtt ttttccgccc caagcgtcaa cgatttcttt
    24181 tgggatatgg atatcaaatt gctgaaatcc ccaaagccag ccatcacgat acgttttttc
    24241 tgtgtcaact aacaaaccat ctaaatcaaa tattgcacct ttcatctata cattaatcct
    24301 ttccaaaaca atacacatag tttagcacga ctggtccaaa aagaacagaa gcaaagaacc
    24361 agccttctcg tttataagag ttcttggaat cgacggatat agattttctt cacagctgtc
    24421 acaagcgcca gataagcgat gactgtagca aataaccata accagtaatt gccaggaagt
    24481 ggcatcaagc ctaattcagc accaaaactt gtaaaaggaa ggacagaacc gacaataatt
    24541 cctaatgtag tgataacaaa catggcaaat gttgcgttgc tttggataaa tggtactttc
    24601 ggtgtacgca gtgcatggag cactaaggtt tgtgtccata gtgattcgac aaaccatcct
    24661 gcatggaata atgcgatgaa aatcactcgc tgtgaactat ccaatgtatg gaaacttccg
    24721 cccactacag caggacaaat aacaaaatac atcaacaagt aagtagtaat atcaaatatc
    24781 gaacttgttg gaccaaagta aaccatgaat tccccaatac tagatgcatt ccattttttt
    24841 ggctcgtcca aatattcttt atccatccga tcccatggca aggaaatgca ggaaacatca
    24901 tagatcagat tcaaaaacaa aagttgtaaa ggcagcattg gtaaaaatgg caggaacgta
    24961 cttgcaatca gcacggaaaa catattgcca aaattagaac ttgctgtagc cttgatatat
    25021 ttcatgatat tcccaaaagt ttctcgtcca gaaagaaggc ctctttccaa aatcgtcaaa
    25081 tctttttcta aaaggatcac gtctgcagat tctttcgcaa tatccacagc tgtatctaca
    25141 gaaataccga tatcagccac tttcattgca ggagcgtcat tgataccatc tcctaaaaat
    25201 ccgacagtat gaccattttt acgtaaagct gtcgtcaaac gtgctttttg ctgaggattc
    25261 aattttacga atacttcatg gtcttcagct acttgtgcta attcatgatc actcatttcc
    25321 gagattttct caccggtaac caattcattg atctctagac ctacttgacg gcacactgaa
    25381 cgtgtaacta attcgttgtc cccagtcaga acttttacag ctacaccatg ttctttcaga
    25441 gctttcaacg cttgtttcgt tgtttctttt ggtggatcaa ggaaagcaag ataaccaatc
    25501 aaaaccatgt ctgactcatc tttgactgaa aattctccaa cgacgcttgg attggtcttt
    25561 tgtgcgacac caatcactcg taagccatct tcattaagtg actgtacttt tgcaagtacg
    25621 gcttcttttc gttcttttgt taatggtgta acagtaccgc caagatcaat aaatttagaa
    25681 atgttcagca tttcttcaat cgcgcctttt gtgatcattt gtgttttacc agctggatca
    25741 gcaaccacaa cactcatacg acgacgttca aaatcaaaag gaatttcatc cactttttga
    25801 taattgatct tatctgtatc taaagtttct gttgcttcat caatgatagc cacatctaac
    25861 aaatttttca gccctgtttg atagtagcta tttagatacg catggcgcaa aacacgatca
    25921 tcttcatttc cttcacaatc catatgatat tcaagaatga ttttatcttg tgtcaatgtc
    25981 cccgttttat ccgtacataa cacatcgatt gcaccaaagt tctggatggc attcaaattt
    26041 ttgatcactg ttcctttttt ggccatcaca cttgctcctt tgaccaaatt cgtcgtaacg
    26101 atcattggaa gcatttcagg tgttagacca actgctacag atagtccaaa aagaaaagct
    26161 tctagccaat ccccttttgt aagcccattg atgacgataa ctgctggagc catcaatgac
    26221 ataaatttga tcagtagcat ggatgtttta tggatgccta attcaaagct tgattgaatt
    26281 ggtttttcag atagcgtttc tgctacatga ccaaatagtg tttcatttcc aacagaaaca
    26341 atgattcctt ccgcacttcc actgatgaca ttacttccca taaagacaag attttcgtaa
    26401 cttgtttcgc tttgctgatc gtcttcacat actttcgcat gcttttctac tggatagctt
    26461 tcccctgtca atgctgcttg ggaaatgaat aaatcctttg cggacaataa acgcatatcc
    26521 gctggtatca tatcccctgc tgaaagccga acaagatctc cacagacgat ttcttccata
    26581 ggaatctctg tgtactttcc attacgtttt acagctgccg ttactttcac aagtgatttc
    26641 agtttttcag ccgcttgatt ggaacgtacg gactgaatca acgtcattgt cccgctgacg
    26701 ataaccatag tgaagataat ggctacaccg aaaagatctt tatctccagg agctgctaat
    26761 acatattccg tgatgaatga aataattcct aatgcgatca acactaatgt aaatggcgtg
    26821 atgtatgctt tcaatatttc tacaatgaat ggtgtttttt tgccatatga aatcgcattt
    26881 tcaccgtaat tttctcttga acgttcgatt tgttcttctg tcaatccatt tgctgatgca
    26941 tggaatagtt caaataattc ttgtttcgtt tttccagcaa aagctgcata gattttttgt
    27001 gtatctttct ttttttccat ctcaatctct cctttgtgtc caaaagacag ctgttctcgc
    27061 tgcttttggt ctttagctat tcatcggatc gatagagaaa aaactgaaag gggctgagaa
    27121 cagatagata atagccctgg cctttcctct agatccttgt ttttcgaatg actgtcatcc
    27181 atttgttctc atctccttta ctaagtaatt ttaattcatg acttccaatc atttttcgaa
    27241 tagttatttt tccaattaga ccacctcctt tttatttttc accgatgggg atttgcggca
    27301 acaaaaaaac ctctcgccaa atactcagcg aaaggtttga aaaagcatat taaaaaaagc
    27361 ttctcagatc tcatcgttga gttttgacac tgtacaacgt aaaaagcaga tacctgcttt
    27421 tagctacctt atgaaaagcc ttatctcgac aattcctgtt ctacccattg gcgtctctcg
    27481 acgtttttgg gcagtagcct atgtttgtat aggagcctca cctaacaaat cttattcggt
    27541 tttccttcgt taatataaca actaaaaaag aggatgtcaa atcaattttc catgtttttc
    27601 atactttttt taaaatacac acgttatatt cctgtctcac gcggttttat ttgtctcatt
    27661 ttccacctcc cgctataata gaaatatagt aggaaacgag gtgctgttat ggaaagaaca
    27721 cttgttatta tcaagccaga cggcgtgaga cgccatttag tcggaagcat catccaacgt
    27781 tttgaagcaa agggactggc cattgctgaa atgaagtttg aaactatgac tcctgaatta
    27841 gcaaaagaac attatcagca cttgactgag cgcagttttt ttgatgaact gatcgattac
    27901 atgacttccg gtcctgtcgt ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc
    27961 cgtaagatgg ttggagcaac aaaagctgcc gatgctgttc caggtacgat ccgaggagac
    28021 tacgcgctgc cggggactga aaacatcatt cacgcttctg attcccgtga tgctgcggta
    28081 aaagagatcg cccgcttttt cccgccattc gatataaaca aaccgtctga aaaagcggcg
    28141 aagtgataca aattctaaac tgctggtcta gttgctgggt tcagcaatgg ccagcagttt
    28201 tttctctaag gacctccgtt gattctttcc taacatatat tcgagcactg tgaagaattc
    28261 ttggtagtct ttcgtctttt cttcccctaa agcagcagaa atcaatcttt gataataatc
    28321 tgccatcaat gtttcataga tcactgtact tctttcttta tgtgctaagc tccgtttcat
    28381 atcttcattc acactacgtg cctttttcaa ccgaccattt tctatataaa acgaagtcag
    28441 gttcattaaa aaagtaaaga gatactgctc atgattgttc aactgtgcta atcgtcgcat
    28501 tctttttttg attttcaaat aattgttctc aatgaaccga tcactgaata tccaaggaaa
    28561 atcagctaga atagatagtt caaattgacc atagtctggt atctgcatca agtattcttc
    28621 aatgattcgg cattcataac gtaaaaattc aaattctgtc tcagatactt ctgttttttc
    28681 gaccatgtga agattcaaaa ccaaataata cagatcttct gttttcttaa aatgcctacg
    28741 gttattgctg atctctttat aaggatcaaa gctgtcttct tgtacccgat ctagcattcg
    28801 tctttttttc tttaacttta ttggttcatc cagctggttt cgcatccatt catattctgc
    28861 aagtgtcatg ttcattcgct ctaaaaatga tctcaatgtt acaaatgaca tttttttcac
    28921 tgaatttgtc tcaatcccga ctagagaagt cttaaaacag attccttccg ctaattggga
    28981 ctgtgtcaac ttcttgttga ctcgaagttt cttgatcaat tcaccatcat acataatagt
    29041 tctccttttt aatttaaatt tttccacatt caataaatag atacgttaaa aaggaaaaat
    29101 acacgaaaaa aataaaaaaa tgtttttttc ataaaaaata ccaaccttca agcattagaa
    29161 tgttgtctaa ctactgaagg ttggtttgat tgtttattga ttattttttt gctgttcaac
    29221 gtctgttctt gttactggat ataagttggt tgttgtttta ttaccatttt ttgaaaagac
    29281 acttgtcgaa tgttcgcctt tttgcttttc gattgcttca ttacgttccg cttcattttt
    29341 atagtcatat tttttcggat caatcgtctc aaatccgttt ggcacataaa accgcaagag
    29401 attttcctga ttcagcttat ctgaaagact taacgcagtc ttgacttttt cctgatcttt
    29461 ggagatttcc tcatcgattc ctgctgcttt cgtatcgatg atttctcctg tctgattatt
    29521 gtaagctttt cctcctagga cggtgtattt gggtgtcaca aagttcccat ttcgaaaagc
    29581 aactacttga tcatgctgag aagataataa gtctgtacca aaatgaagat aatctttatc
    29641 atcgattccc aatagatgta gtaatgtcgg caaaacatcg atttctccac cgtattcatg
    29701 attgactgtc cctttagtat aaccaggcat atggatcatc aatggcactc gttgcatctg
    29761 tgcgttgtca aattcatccc atgtatctgg atctttacca agagcgcttg ctaaatcttt
    29821 attatctgta ttcgagatcc caaaatgatc cccgtaaatc acaaagattg aattttggta
    29881 aataccagat tttttcaaat acgtaaaaaa ttgttccaat gattgatcca gataatgtgc
    29941 cgttcgaaca taattatcta ctgtgctatt gccagtattc aaagatggaa agtcgaaatt
    30001 cttgtcatcc gtataataag gcgtgtggtt ggttacggaa agaaatttcg tgtaaaacgg
    30061 ctgttgtaga tgctcaagat attgggcaga ttctctgaat aaatctttat ctaatatccc
    30121 ataacctaac gtttcgtcgg attcatcaaa ataagatcga tcaaagaaat tgtcataacc
    30181 taagttttta tatacatgat ctctattcca aaaactagct acattcccat ggaaaacagc
    30241 gcttgtatag ccttgctttt gtccaaggat cgctggtgcg gcttgaaaga cattgtctga
    30301 cccaagttct gtgaataatg agccttgagg taaaccaaat gtgccagttt ccaacatatt
    30361 ttcagcatca cttgtctttc cctgtcctac ttcatgaaag aaattgtcaa agctgatcgt
    30421 tgcttgattc tggaaaatac tatttaaaaa cggtgtgact tcttgtccgt ctaccttcat
    30481 attaatcaaa aactgctgga aactttctaa atggagaacg atcacatttt tccctttagc
    30541 gatcccaaat gtttcgggat ttggctctgc ataatgtttc tttgtataat ctaaaacttc
    30601 atcaatccca ttactgcttg catgcgcgcg aacactgcta gtcattcctg ttttgattcc
    30661 gtcataaata gtatacgcat cgattcctag atatttgaca atatatgaac ggtcaaatgt
    30721 tcgctgcagc aattgtggac ggttggcttc gctcagcgct agatttcctg aaaaaataag
    30781 ccctgaaaga caggtaacag cgatagccat cggtttgccg acagggttgc ttttgatagg
    30841 tattttcttg accttcaacc aaaccagtag cccaatgaac acagcgatat ccagccagta
    30901 gatgacatca tggggcttca tcaaagaaaa ggagcttcct gacaatcctt gcgacacttt
    30961 cgaaaagcct aatacagatt tgactgtaat gaaatctgtg aattcccgat aaaaaataat
    31021 attcaagtac aacaaaagcg tatttaaaat atcaatgatc aaaagaatag ctaacgctgg
    31081 ctttggcttt ttaatataca aagaaaggcc aaaaaacaat aaggtagtcg caattgggtt
    31141 gatccacaag ataaagtact ggatcgtacc ttccacacct aatgaaaaat ctgcatagta
    31201 ggcaatgatc gtcttcagcc agaaaaagaa aacagctgtc aaaacgattg ctaaacgatt
    31261 ttggcacaat tttttaagtt gtgtcacgta tatcacttct ctctattcac attttttctt
    31321 attttgctcc tgaaaaatga aaatttccta aaaaagttgt tatcttaaga tttttttatt
    31381 ctttctaaag attattccgt ccacttttca cagcataaaa ccgctgcgat gtaagcgatt
    31441 aaactaatga agagataaaa gtcttgtggt gaccgcttca ctccacggta ttcaaaagcc
    31501 agtttctcgg tattcgtaca aaaaaagagg ctgtgacaaa attcttgtcg cagctcctta
    31561 tttcttgatg caggacagct ttttcacaac ttcttcctta aacggtgttc tcctgccaac
    31621 tcctcggcca caagtcacaa tatttataaa acatgagaag ctgttttttg aaattgttcc
    31681 ttgtggctca gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct
    31741 gaccctcggt attagattat gtcaggacgc taattttaca acctcttggt aaacggtgtt
    31801 caaaagcttg catctgcggt aataagccca accaaacgaa aaatataagg agctattttt
    31861 tgaaattgct ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt
    31921 gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    31981 gccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct gtttttcatt
    32041 gtccatagga ttgatattgt ccttgtgatg actcattcgt aataattgag gcttgatttt
    32101 cttcacttcc cgttatcgtt tgtccactgc cagaatctga aggacttgtt cctgtcgact
    32161 cgtttttttg agaagagaca gttgttttct tgacttgttc atttgtcttt ttccattccg
    32221 ctaaacgatc ggcgaccact tttttgtcta ctttttcaga ataagcggtg atcacttttc
    32281 catttttcaa acgggctact ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct
    32341 tgtctttaaa ttgatattgt ctgagagaaa tcgcactaag tacgtcatcg atattaataa
    32401 attgattttc ttcttttcct tttggcaaat aaatgtccaa ttcgcctttt tcgtaattgg
    32461 cagttatttt atactccgcc caccaataag ttgagaattc ttttttgaaa ttatctactg
    32521 attctttgct tgctacgatt ttttctgttt ctttctgagc aagatctgct gtcatttttc
    32581 ctgaaaatgc tgcggataaa ataagcagta agaaaaaacc gaataaccaa aagagaagaa
    32641 acggtctttt tttcctcatg ctttcctcca ctcctattta attttgacta ttctattata
    32701 tacaaaaaat gaaatagaaa gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct
    32761 ttttcacaaa atggtaagaa tggagaattt cacgcatttg ctctcaacta ttctttctta
    32821 agcctcaaat ccgtttttat ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt
    32881 tctttttcca tcttgtgcta agtctactga aataaagcca tatcgattct tatatgcatt
    32941 ggtccacgac cagttatcca tacatgtcca catatgatag cctaaacaat tggcaccttc
    33001 ttgaatggct tgatggacat atttcagatg agattggaca aattcgatcc gataatcatc
    33061 gtggatcatg ccatcttctt tgataaatcg tgcttcgttt tctacaccca taccgttttc
    33121 agaaataaag cagtcgatat ttccataatg atctcgtaca ttgatcaaaa tatcatagat
    33181 tccttgttca tagatctccc atccgcgata aggattcatt ttacgaccag gcatttcata
    33241 attgtcgaaa taatcctcag gcattggacc gtttttagta tcgattggtg tttcttttgc
    33301 ttttacccgg cgtggctggt aataattgat acctaaaaga tctactgtat tttctttgat
    33361 aaccgtcaaa tcctctgcat ggatcacagg catgagatca agttctttta ctatctcaat
    33421 caattcactt ggaaactcgc cttttacagc tggatcaagg aatgaacgat tgaagaacgc
    33481 atctgctatt tgcgctgctt taacatcttc agggtttgat tcgtctcttg gatagcttgg
    33541 tgtcagattc aagatgatac caataacacc accttgctcc atttcatggt aagatttgat
    33601 ggctaacgct gaagccaacg cttcatgata gccaacttgg atcgcttttt tcatatctga
    33661 ctcttctgga taatgccatc gatataaata acctgcttcg actggaacga ttggttcatt
    33721 atgagtaaac cattttttta ctttcgaacc aaacaattca aaacaacttt tggcatattt
    33781 ttcataggct tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca gatacatcgg
    33841 catatcgaaa tgatacagat tcacaaaggg ttcgataccg ttttcaatca actcatcaaa
    33901 ataacgatga tagaaacgaa cggcttcttc gttgattgtt ccagattcat caggtatcaa
    33961 acggctccat tggatagatg ttcgaaatga attatgccca gtctgtttca tcaactgtac
    34021 atcctcttga tacttcgtat aaacttgcga ggttttttca ggtcctactt gatcaaagaa
    34081 cttttgcggt tcttttttgt accaataatc aaaaatgttc tctccttttc catcgcctga
    34141 aatccttcct tctgtttgtg ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa
    34201 ttgatatttt ttcatcaaag cacctctttt tcttttttag ttcgttttag ttgattccat
    34261 gtatgatatt ttccatcaca atcacttttt cattttcttc ctcttctttt ttgattcgtt
    34321 ttaacagctg ttctgctgct cgttccccga ttttatctgt tggtggcgtg atcgtgattt
    34381 tcggaaggat actcagatcg tttttatcta aataatcata gtgactgaac gcaatatcct
    34441 tcccgattct gacaccttct tgattgaaac attcgatggc accaatcgac atattgtaat
    34501 tgctgacaaa aatagcagtt gccccttttt ctaataatgc ttttgccccg taatagccgc
    34561 tttttctaga atagtctcct gtaaagacct ctaattggat cgatggatca ttttggaccg
    34621 cttctttggc tcctgctaaa cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa
    34681 atccgatttt tttatgtcct tgttcaccat gatgagcgat cacttttgct acactttctc
    34741 gatcattcac taatattgta tcaatacctg ctggtccttc tggacaattc aatgcgataa
    34801 ctggaatact catttttgtg agcatttcaa atccagacca ctcttcttcg gataagaaga
    34861 caatcagccc atcgatcaca tgcttcatca ggtattcaat tttttgttca attttttctg
    34921 gctgatcggc aaacccgctt aataataagc tatacccatt ttcttcagct actctttcga
    34981 tccctgagac aacttccgac ccaaaacggc tggaaatccc gtttatcaaa agtccaatag
    35041 agaagcttcg attattcttc aaccctttag caatgatgtt ttctttatag tccaacgctg
    35101 aaatcgcttc tgctattttt ttcatgttct tttccttcag ttgctgacca ttcaagtaac
    35161 gggaaactgt tcccactgaa acaccagcca tcttagccac atcacggatc gatgccatac
    35221 attctcactc ctgccagtta catttgattt ctctttcttg ttgttcgatt atgatgaccg
    35281 cattctttgc atagtgttgc ttgtcatcaa tatccatata actaaagtct tcagtagagc
    35341 aacttcctct cactattaat tatacttaaa caagtatttg aaccggttca tttttaatat
    35401 agcatttttt taaaagattg caacactttt ttaaaacgct ttctgaaagt ggcggatttt
    35461 ttgccttttt aaaattttat tctaaaattt actctatttg tgggtgtttt gtgcgttttt
    35521 ctcttccatc cgtttgaagt tttacattcc aatggtataa tttctttatc ctatcaaatg
    35581 ggggaagtat tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat
    35641 tgactggctg tgatttgttt ggcggctctg accaatcctc ggacgaaaca accgattctt
    35701 ctgtcttaaa agcggaaaaa gcagatacca ccaacagtgt atatacgcta aaagtacgta
    35761 aagatatgga tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac
    35821 gcacgtatca gacacaagga actgattctt ctgaatcatc tgaagaaaaa acagtgctga
    35881 cagatatcaa gatcactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa
    35941 aagaaacaac agtcatattt acaaaaatcg gtaattaccg tttgaaggat gaggatggca
    36001 acatctacag tctatagtgc cgtctgttgg atcttctttt gtttctcccc ttcttttctg
    36061 ctatagtaaa gaagcaagat gattgtaaag gagacgaaat ggatgatcga tagtcctgat
    36121 tattgtacat gtaataacaa caaaaatatc catacagaat ttaatgacca aggaagcttg
    36181 agcatatgcg acaactgtca caaactagtc aaagattcat ttaaaacatt tgaatcttat
    36241 cctgttcaaa attatgtaaa aagtagaaga agataaacaa atccaagcaa aactcactat
    36301 ggctgatttt tatgaacttc aaactcagct tcttcctttg atcaagcttc atcagtaaaa
    36361 caaacagtgg atcatttcag tttacaaatc aaagagtaaa gatttattta tttcaaatat
    36421 ttgaatataa aataaccaaa cgatagaaag gcttattcta catcacgtag taatttttct
    36481 ttcccgtcgt ttggtttttt atttttagcc taattcttga ccttttgcat gctggttatc
    36541 cgtaaaatat tttccagcag gctttccttc caattcagct aatttttctt ggatctgacg
    36601 gatcaccttt tttgcatctt ctggattttc tacaaaatcg aatttatccc catccacttg
    36661 tattttagga catacatcaa aatcttcata ccattgatta tagcgggtgt tcaacgtttc
    36721 ataataggta tataacgtct catcgctttc tagctgttca tagtcacgcc cacgtttttt
    36781 gatacgttct agcatcgtgt ccaaagaaac tttgatatgg accatcaaat caggccgttt
    36841 ttttggagca acatcatcta actcgtttag catcgtatct aataagctat cgtactgctg
    36901 tacttcgata tccgttaccc gacctaaatc agcgtttaga tggaacaaca ggctatcttc
    36961 atagatggat cggtctagca cattgtcatc gttcactaaa gcatttttca ttgctaaaaa
    37021 tcgcttgttc aaaaagaaga tttgcaataa aaaagcgtac tgctcaggat ttgaataaaa
    37081 gagcggcaat acttcgttat cttcgacatt ttcataaaat ggtttagaat caatctcttt
    37141 tgcaagcata tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa tcaaaatcac
    37201 tgacatacaa cccttcctct tctataagta ctcgcctacg taagtttcaa caggcaatgt
    37261 ttcaaaatca tattcacgta cagcttggtc tgattttaaa tacgtatgga gactttgtgt
    37321 aatcatcaag ttcgtaggta ccgtctcttc ttgtacaatg atcatcggtt ttttcaacat
    37381 agttgccaca cctaattcgt aagccgttcc tggatcgatt gtttgatgct caaagtcaag
    37441 gatagcaaca acaaattctg catttgtcag ttcttccata tctttttcgt atacttcctg
    37501 cgcccatcca gcgctgaata gttcataatt gctttcttgg tgatgacgtg gagagtagaa
    37561 acttgtgact gttttgtttt cctccaacgc tttttcgata cgacttactc gatcgatttg
    37621 ctcctctgaa aaaaatggtc ctgctaaata tatttgactc attattttgc ctccttcaat
    37681 aataactgat ttttaataga tactgctcgt aattcattga ccagttccaa tggattgttg
    37741 gaaaagatta cttgatccaa ccatttttta tccataaaat cctcttttgc aaaagtttct
    37801 aataattgtt gcaagccgct gtaataaccg ccaatattca atattccgat tggcttgtcg
    37861 ggcgtcagac cgattgctat ccaagaaaga agttgtgaga attcctccaa tgtacctaga
    37921 cctccaggga aaacgaggac agcatctccc ttttcaaata ataatacctt tctttcatcg
    37981 atcgtttctg taggaataaa tgtagtaaca tcttttccag gcaattcagt ttcaaaaagc
    38041 ccattcggat agatacctgt gacttttccg ttattttcca gtactgtttt actaatcact
    38101 cccataatcc cggagtatga accaccgtaa accagttcga tttcttcttg tgccatgtat
    38161 tttcctaacg tttgcgcaat aaatttgtaa ctttctttat taccaaaacg ggaaccgcaa
    38221 aaaacagtaa gtttcataaa aaaacactcc ctaactcaaa agtctatctt cattctacac
    38281 gtgttttttt aaaataaaag actagatttt gtagtttttt agtggtatat aggatagacg
    38341 ccactatata tagtatttag aaaaaaatca tttttcagcc attttataga taaaacaaaa
    38401 ataaataacg tgttttcatc gatatgaaat gacttactta tagaagatac tgggatttcg
    38461 tcctatatgc caataacgtg tattatttta aacaacaaat acttgtttct tttattaacg
    38521 aaaaaatcat tttgttctcg tgttatgttt cactgataaa tgatgtttct ctaaataaaa
    38581 aagccgacga actaaacgat caaaatttga tcatttttgt tcatcagctt ctcctaaaaa
    38641 tctttttgtt aacgataaat agctggattt gtaaaaatat cacttgaatc gtcactgctg
    38701 gaagaaaatt cagtgataac cgcacctgtt gcgcctgcct ggaactagtg tttgatattg
    38761 ggctcgaacg tgtactgatc tctagcatga agaataattt ctttttttac tgtataataa
    38821 ctttctttcc tttttggaat agtacaggaa agtttttttg tttcctttcc ttcgacataa
    38881 ggtataccag accctttcga caacgaaagg ttctcatttt tccttcatcg tcatttcaag
    38941 gagggtgccg atgctctgga cagtttcgat cgctcctaaa ccaaaatctg tatattcgat
    39001 tctttctaac tctgattctt tcataccatt cctgtctcaa ggtacatcta ttgtactttt
    39061 ttcttgtaat ccattctgcc tctcctaact aattgtcaaa tcatcccaag atatgtcttc
    39121 ttcctctttt tcttcttgga tgatttctgc attcggatct actggttttt tcaataataa
    39181 aatggttatt cctgtgacaa aagaaccagc taacaatgca ccaatgaatg cccatggtct
    39241 ggtcatcgta gcgactgcaa acattcccga tgccggaata cggctatctg cgccccaaaa
    39301 catgcttaat ccgccaccta ctgtcccacc gactcccgtt gctatgacat tgcgtgccaa
    39361 gtcgttcaat acgataggga aacatccctc tgtgatctga cagatcccca taggaaaagc
    39421 ggtcttcaat gtatcgattt cttgacgatt gagtatattt ttacgaatga ttttccctaa
    39481 gaaataagcg atcgtcaagc cgaatggggt taccatcgaa gctaaaatca atacagtgat
    39541 tggttcatag attccttctg aaaatcgtgc cacaccccct tatccagaat aaatggtaat
    39601 ccagaaacag ccgtgtatct gcggtaataa gcctaaccaa acgaaaaata tgagaagcta
    39661 tttttgtttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctcttttttt
    39721 atttatgttt cataatcaat atggtaagga tgcagctccg tatggtagac cgcctccgaa
    39781 tattcaatca tttccccaat ttgcgtagac gatttccgta tccgtttgat gccgatctgt
    39841 gcgtctgttt tcagaattcc ttgttcttct tctgttaaaa atactgccga gaaatcatca
    39901 gaaaatgagt caatagtata tccttttttc gtcagcaaac gatacaaaga ttctttgcca
    39961 aattcttctc tgctgaattg catcatttct tgtggaagat aatgaacaaa ataaatataa
    40021 ggctgattgt tcaaataata taagcgctta aaacaaacag cttgctcacc gaaaaaggaa
    40081 aacaaaggat cttccttctt tatatggacc aattgcaaat ccagcgtttc tttccggact
    40141 tcatatcggg agttctccaa gatttgcgta aagcttgccg ctttggataa tcgattgtaa
    40201 ggacgattgc ttagaaccgt cgtcccacga ccacttttct ttgcgacata ttcatctgat
    40261 gccagcagtt cgattgcctt tcggacagtg attttgctga cttgaaacag ttcttctaat
    40321 tcactttctg tcggcaacat cgaaccgact ggatattttc ctgagaaaat atcttcttta
    40381 attttatctg cgacttccaa gtatagtaca cgtgtccttt tcataacgtc accctttgct
    40441 ttactattac taaaagaata gcacataaaa aataaaaaaa gaacacaaat cacatgtgag
    40501 aagtgtgttc tttttcattc agctgcttta attacaagtt tacgccattt gaggcaatta
    40561 cttctttata ccaatcaaaa gattttttct ttgatcgatt cagtgtccca tttccttcat
    40621 cgtctagatc cacatagata aagccgtaac gtttactcat ttctcctgtt gatgcactga
    40681 caagatcgat acatccccaa ggcgtatagc ccatcaaatc tacaccgtct gctactgcat
    40741 tagccatcgc ttcgatatga cgacgtaaat aatcgatccg ataatcatca ataatcgtgc
    40801 catcctcttc catatgatca atggctccaa gaccgttttc aacaatgaat aatggtttct
    40861 ggtaacgatt gtataattca ttcaatgcga ttctcagccc ttctggatcg atctgccagc
    40921 cccattcact tgcctcaaga aacggatttt ttactcctcc cagcaagttc ccttctgaag
    40981 ttgctgcttg tggatcagtc tcattgattg ccgtagacat ataataacta aatccgatgt
    41041 agtccactgg atattctttt agcaactgga agtctttttc cgtttgctct aatttttccg
    41101 gatcgactcc atatttttta tgggtacgcg ctgtgtatgc agggtattct ccacgtactt
    41161 ggacatccgt gcagaaaaaa ttgaattctt ggttttggat cattgttgct gcctgattga
    41221 ctggattggc atcgatgctg taagttgttg catagataat catgcagccg atttggatat
    41281 ccgaacgcaa ttcgtggccg atttttacgg ctttgctact tgcaacgaat tgattatgcc
    41341 atgcttggaa aatattttgg tactctcctg ctccattgct ttttactaat ccttgactta
    41401 aagctggaaa gtgaaaggcc gaattgattt cattgaaagt catccaatat ttgactttac
    41461 tgctgtatct ctctaatact gtctgagcaa atcgttcata gaagtcgatc agttttcgat
    41521 ttttccagcc accgtattct tttgccaaat gaagaggcat ttcgtaatga gaaatcgtga
    41581 tcacaggttc aatatcatat tttaagcact catcaatcag ctgatcataa aattctaatc
    41641 ccgcttcgtt aggagtacct tcgtctccat ttggaaaaat tcgtgtccag gcaatcgaaa
    41701 aacgataaca tttgaatccc atttccgcaa acaaggcaag atcttctttg aaacgatcgt
    41761 aatgatcgat tcctcgatga ttcgggtaaa tatatttttc ttgatcaatc gtccagtcaa
    41821 attcttcact gccgataatc gcaaagcgct gcttacctcc aggcatagca tctgcaacgg
    41881 atagtccttt tccgtctcga tcataagccc cttctaattg atttggcagc agttgctcca
    41941 ccccataaaa atttttctga aaaacgtgat tgcatcctga tttcctcctt attttacaat
    42001 tgccagaaca tcttcgtttg caataatctc tttttggtct aattcaagta tatcggcaaa
    42061 tgcatcagta ttcgtaatga caactggagt gattacgcta tagccagctg ctctaattgc
    42121 ttcgatgtca aaacgaatca ataaatctcc tgctttgacg agatcccctt gtttgactaa
    42181 gatctcaaag ccattcccat ttaattctac tgtatccatc ccgatatgaa tcaaaatctc
    42241 cgctccgtca gtcgttgtga tccctacagc atgtccagtt ggaaaaatag tcgtgatctc
    42301 tccgtctgca ggtgcaacta gttctcctac ttctggatca atagcgatac cttttcccat
    42361 cacgccagaa gaaaaaactt ggtctggtac ttcactcaat ggcagtactt tacccgttaa
    42421 aggacttttt aaggtatgcc gtgctgaagt aatcggtttt cctgcattcg catgtttgtt
    42481 ttctaattga ttgtcttgtg tttgttcgtc aaatcctaaa atcaatgtac ctacgaatgc
    42541 aagaacaaaa gcaattcctg tagctattac tgctactgtc acatttgact ctacaccatc
    42601 caccgtatta atgaaagtcg gaatcgtcaa taagctgacc aaactgctgg agaaagcttt
    42661 tactccggaa aatccgataa tagctccacc aatacctcca gaaatacaag cggcaatgaa
    42721 tggtttcttc aaaggtaatg tcacaccata cacagttggt tcagtgattc caaataacga
    42781 agtcactgta gaagaaatcc ctaatgctcg aagtttcgta tcttttgttc gcaatgctac
    42841 agctaaagca gccccacctt gtgccaagat ggctggaaga agcattggca tcaatacatc
    42901 aaacccatat tgttcgatat tcaaaaacat gatcggtacg aatccccaat gcatgccgaa
    42961 catcacgaat acttgccata aacttcccat gattagacct gctacgattg gactaaatcc
    43021 ataaatcgag ttgaagagac cgcctaaagc attaccggca accgtcccga ttggtcctaa
    43081 agcaagaaat gttaatggaa ccatcactac taaaacgaca agcggaacta aaatcatttg
    43141 cagaaattgc ggaatcactt tttttacgaa tggctctaat ttactttgaa cccatacggc
    43201 tagaatgatt ggtatgacag aagaagtata accagatggt gaaagaatca ctgggatccc
    43261 aaagaaactg atagcttctc ccgcaccagc aactgctgtg atagctggat acacaagtgc
    43321 catagcaatt gctactgcta aaaactggtt ggctttgaac tttttagctg ctgtaaaagc
    43381 aagcatgaca ggtaagaagg taaagacacc atctgctgca gcaaacaata tttgataagt
    43441 accgctatca gctgtcagcc agcccatcac cgttgccaat gataagaatc ctttcaatac
    43501 cccggcggct gccatcgccc ctagaaaagg tgtaaatata ctagagataa tatcgatcag
    43561 acgattcaaa atatttttgg actcttcttt tccatcctcc gaaactgcaa gaccttcctc
    43621 atccacgatc gattgataaa catctgctac attactgcca atcactactt gatactgacc
    43681 gccactttgt acgacttgga tcacgtccgg gtcttctttt aacttttgcg tatctgctaa
    43741 agattcatct tttaatctga atcgcaaccg cgtagcacaa tgtactaaac ttttgacatt
    43801 cttttgtcct cctaccgctt cccatacacg acgaccaact gcttgattat ccattgtttt
    43861 ttccttcttt cttttttatt taaaaaataa aaacccagat tgaaaaaaac agtcacaaac
    43921 attcgtgaac ctattttttc aatccaggtt ttgcctgcca tacagtaaca atccttatga
    43981 ttggatgctt tttttctcac ttactactct ttgaatatga atcgttaaat aaatctgctc
    44041 tgcgatcccc atttcctgtt tttgcgtttg tcgaagatac tcattgatcc gttcgacaca
    44101 atgaaacgct tttggatact ttccttgaac taatgcgtag agaaattcat cttgctcgtc
    44161 agatccttcc cctttgagat aacgctgggc aaaaaactgc agatgagtaa tgatacgctg
    44221 ataattcaaa gattcttcat cgaacacgat gccgaagaat ttactgatga tactcagtat
    44281 atctcttacc attgtggtag cggtcatcgt cacttgcatg tcttcgtttc cttgttggct
    44341 attcacaaaa tgaaaggcaa taaaaccggc ttcatcctca gaaagttcca cacccgtctt
    44401 ttgtttcacg aacaaaagag cctctttcgc tgcttgatat tctttcggat aaaatttttt
    44461 tgtttcaaat aataatggat taggcagttg gatattttct tttgctcgtg tgatcgcata
    44521 atgaagatga tcggtcaatg tcagataaat attaggcgaa aattcaactt ttagtttatc
    44581 ttcagccagt tcgactactt gtttgaccca ttcgatctcc tctgccggaa tatcagccat
    44641 cagctgctgg aattggtttt tagcaaaaga atctcttaaa acaaattctt tttggaccgc
    44701 ttctggcgaa atctcgtccc caatcttttt ttgaaaagct agtccttttc ccatacaaac
    44761 gacttcttcc ccgaatccat ttttcgaaat gacgacattg ttattcaaaa ttttatcaat
    44821 tttcattttg cttcgccctc tcaagaaaaa taaaaaaacc tagaattcgc ctactgtttc
    44881 ttagctgaat tctggttttg cctgccgtac agtaacaatc catttcttat tcaaaatcat
    44941 cataccatcg aatttttatt tatgcaagcc ttttcatata aaaaataaaa aaactggaac
    45001 acaagtctat ctagaaaaca accaaatcag agatgcacct ctttgcgttt ctcttttttt
    45061 cagcaaattg gtttttcctt atcgattcat atcctttggc tgtttttaat ctgtaacgac
    45121 ttatgtccca gtatcctaaa atgtttttgt ctctagattg aaagcagatc tcatatcatc
    45181 tgtcgcatct tttatcgatg taacactttc cagttctcct tgtacaacta gtcgcgtgac
    45241 ggcgtccatg tcgccacaag taattagtgt gacaatttct tttccctcga cttcattcag
    45301 taattgggta tctgtaggag caactttttc tttaaaaaat gttttgtatg tatacacatt
    45361 ttctaaatct gtcaaataga tcttggcacc catctctaaa ttttctagcg gtgtaaacag
    45421 caattcaggg ttcgttgcac gatggcttgc taaggcataa tttccttttc ccatttcttg
    45481 atctggagat aatgttcctg ctccgtaaag tagtgacgta ttatccaagc ctttgaaaac
    45541 cggtaaattg attgaaactg aagggactgc gatactagca atcactggta gatctgttcc
    45601 tctcatttgc gacctcatga ccccttctgt cgtcattggt tctaccgcat caaagtcaaa
    45661 actgacatct ttatcgttat tctttttcag atcttctttt gttacattcg ctatagcata
    45721 tttatctcct gtctctctga caaaataatc cttgatctgt tcattgaaaa tcaaagctag
    45781 tcctactaat aacaagagga acaagaggat attgactagc catctttttg cttttccttt
    45841 ttttttcatt ttctctccct ccagactcat tttactgttt ttcttttgct ctttccaaca
    45901 gccgaatgac acatttctac aactctgtgt atttttttcg tacacctgcg cttttttcta
    45961 caggatattt caaattattt ttgatcaatt tttcttcaat gatctcatct agatccaggt
    46021 gaaggttgtc tgccatcatg tatgaataga tcaatacatc tgccagttct tctttgattc
    46081 gttctaattg tgtttctttt acttcttccg gcgttttcca ttgaaaaagt tctaacaatt
    46141 cagatgcttc caaagaaata gagatagcca aatctttttc gttatgaaat tgtcgccaat
    46201 tacgttcatc tctaaactgg ttgacttttt ccatactatt catttgcttg ccttcttcca
    46261 atcacttctt acttgattaa tcataccatt tttttacata aaaaatagat gatgctttta
    46321 aaaaaactcc tgttttcttt tatttaaaat aaatgtcata gaaaatcaat aatttaggta
    46381 gccaccatgt gtatgcagta gcgtgtcttc ttcccttgag gctttttgtt tttcgatacg
    46441 ttcttcttcc caaacagaca acgctactag aatctctctt aatgattttc cttcttcggt
    46501 gagcgagtac tctactttag gcgggacttg gttataggct tttcgatgga tactttcatc
    46561 ttgttctaac tctcttaact gctgggtcag catttttttt gtgatcgtag ggatttgctt
    46621 gcttctccac gcctaaaata agcgatattg ctcttccctt tatctggtgt aagcaaagca
    46681 gttagcattt tgacgatcgt gggttccgct tttcacttct tatctactta gtatcatgaa
    46741 gtatactttc gtactaatac taagtagcgg aagtgtcaac tactttgttt aactacatag
    46801 caaaagaaac agaagggtga caacggtcat cacgcttctg tttcttttga atatgctgtg
    46861 ttgttgtctc tatctccgtg tagactttcc tcaaacttat cctttgacag taagtcagta
    46921 ggctgatttg ctctttctag atgccagata catttctaca aaaaaatagt gattctgttt
    46981 tgaaaattcg atttttagtt ctgcattttc gtaaacatcc aacatttttt tcgcactgta
    47041 tagacctaac ccgccatttt ttttagaggt ttttcctctt ttgaccatct ccgatagcgt
    47101 caaattcgtt ggatttgcct tgttcgctat actaaaacgt gatccctctt tttcttccag
    47161 cagattgatc gtaatcggac tctgatcggc tacacgatga tcgaatgcgt tagaaataaa
    47221 aatagataaa cagcgaataa aaacaaccag atcaatcggg attgttttca cttcgacagg
    47281 aataactact tggatcggga ttttctccga ttctgctttt tctagaaatt ttaaaaagac
    47341 acccctgaca gctggggata caatatttgt tagctgttgt atttgcggtt tttcaaaaat
    47401 agctgcagaa tgatcttcta atatttctaa aaattccaac gtttcttttt tattttcctg
    47461 aagagtaatt tttaatgaaa acagtagact tttataatca tgtttgaatt cctctagatg
    47521 atggtaatat tcttcattta tttgatacag ttgaagcgat tcctgatgtt gtctttcttt
    47581 tacttggaaa ctgatcaaca taatggaaaa aaacaaagaa aaaaaactga tgactatcga
    47641 caaaagagat agaagcagat aattagatga gcggaagtga acacttccca ataccaagta
    47701 aatcacgccc cagcttaata aaatacaaat acctccaaaa tagtgttcag ttggataagt
    47761 aaaaagcaag ttgagaaatc tcgtctgttt gtctatactt tttactaata gatagaaaat
    47821 aaatgcgata agtagtgcaa aaaaacttgc tgttccaaga tgtctctttc ctaaataatc
    47881 catcagatca aaaaccagaa aaaagctgag tccagtgata gcaaaaaaaa gattactgat
    47941 caaacagctg ataaacaggt ttttcgtagt aaaataaaaa agaaacatca ataataagcc
    48001 aactaggtaa ggaaagctca ctttagggat aggaagtaaa agtgtcacca ccatcaaagc
    48061 tatacaacca aagctgtttt ttactagcag ttgtttcgaa aagaataaat agatacctaa
    48121 atctaaaatc aaagtaaagg tcaaataaag tgtcactgtt gcgaccatct tattcctcca
    48181 ttgttttgtt tcaaaatgta cacatatttt tttaatagga gcctgcatat gaaaaagtat
    48241 tttatcatag aagataacca gtttcacagc gaatggatca tcagagaatt ggtcaatcat
    48301 catatcgaga atatcgtgca gctcacttct atctctcaaa tcaatcagtt gatccagttg
    48361 ggcactattg ataatcacga tgttttcttt atcgatatcg acttgaagca atcactcaca
    48421 ggtattgatg tcgccgaaca aatacgtaaa aaaaatgcac atagtaatat tgtttttttc
    48481 accgcttata cgaattttgc agtagagatc atcaatcgga aaatccttcc gattggatat
    48541 atcagaaaag acaggaattt atctgaacaa attcgtcaag tgctagaaca gatcaattgg
    48601 aaagaacagc agatcgtaaa tgtcgataaa atctttattg atcaaaaagg tggaagtctc
    48661 gtcctgtatc ctcgggaaat tttatatatt gaaagtatca aaggtttgaa aaaccaagtt
    48721 atggtcaaaa caactacaga agataaatta gtgaatactt ctttaaaaca attgaagaca
    48781 gagttagaaa agtatgctta ctttttactt ttaaaatctt actgtatcaa cttatcgcaa
    48841 ttgcagaaaa ttgattctgc tcattatgtg ttagaatttt tcaacggtga ctccttactc
    48901 gttggtcgaa agatctttga aaagacaaag gaacgtttcc atgacttcca aaaaactgct
    48961 tcgagttgat gaataagagc tgagacgtaa gcatagcaga tttcagggag tgggccttga
    49021 atcggctcca ctccctgaaa tcgttgatat gattttacgc ttcctcttct gtaggagcat
    49081 ctgttaacga ccatgtgaat gttgaaacgt agttgtctac agcataattg gatacaggaa
    49141 caaataattc cactgattgt ctcccttgtt ccacattctc tcctaatgaa taaatccatg
    49201 tgccaatccc ttcatttgct cctgcagtta ctagatctac tgcctgatct gctgggattt
    49261 cgattccttc agcgccagtt ccagaaacgg tagatggcat tccactgcta ttagttgcgc
    49321 tatttcgaat cacggcgttt ttgaatttca cctttgaacc agttagttta ttcgtatcag
    49381 atgttgtacc aataaattct gttcgtttga ctgataattt ccaaccggca taatttccgc
    49441 ttttatccgt tacttgtacg aaattctgta cttgtgtttg tgctccagta ctgtccgttc
    49501 cgctttgtaa tgcagcatat actgtttttg ctgtacctaa cttgattgtt ccaaaatcaa
    49561 gatctggagc ttggtcaatc gtcagtaatc cggatgttcc ctgatttggt tcgtcaggtg
    49621 taaaaggagt atcaggatca tttggatcaa caggaggaac tggaacaacg tcttcaggat
    49681 ttgcagcatc tggtttggtc acagaaatcg ttccttctgt agtggctgag ctcttattct
    49741 cagcaaaaac actttgtgta cataataata gacttccagc tagaacggta cttaatgcaa
    49801 ttgttttttt catttctttt tccttctttc ttttaaaata aataatccag caaataagcc
    49861 aataagaatg actccaacaa gtatcagcca tcgccagtca aatgtttctg gctgattttt
    49921 taaatcaata tccttttcat tcaattggtc cgctgcatca tcagttattt caaaatcggt
    49981 atggagattc cagcgttttt ctttccaagc acccttcaca accgcttgat aatgtcccgg
    50041 agctaatttt tttccttcta atgggacaag taaatcaaaa tttgtattgg gtgctacatc
    50101 tattttttct ttttttattg aaaggtaagg ctcttttgtt tcttttttgt agatttcaac
    50161 cgatgtagtc aagccgaaca tataagaagc atttggattt tgaagattcg ccttaatcac
    50221 atttcttcca tcgctttgat ccgcataagc tttttttaat ttcaaatctg gggaagactc
    50281 tttttcgcta tttcaaatca agattgcttt cacttgagaa aactgattga cgacaccaac
    50341 tgtacttttg ttagaaacgt tctctttttt ttcttgaaac gtgatccctc ctgcgaccac
    50401 accagccagc tctttttctg gcgctttcag tgaaagtgtc tcagtgaccg cactttgagg
    50461 aggaacagtt atttctccca ctgtttttgc taagtttttc atatcataac ttaatgtttc
    50521 atcaaattta ctttttgatt ctccatattc gatcacaccg tttgaattcg tcgtagcgga
    50581 gtggatcaat gtctcaactg ttactgattt atctgttcgg ttaaagaccg tgtaggggat
    50641 ttcctgtgtt ttcccaggct ccatccttac atttatatat gttttgttct tatcaatctg
    50701 attagccggt aattcaaaat ctaccgcaaa attgaattcg gaggcatgga caggtgtctg
    50761 ccaaaggatg aatccaagga gaaggatcat aagtaaagag cagttgtttc gatgactttt
    50821 attccatatt cgattcatca atcatcctcc ttgatcttta ttctgtcggc gcatctgcta
    50881 atgtccatgt tatctctgct gagtaattac cagctatctg attttctttg gttaagacaa
    50941 gtttaaacgc ccgaccgtta tttaaatatt ctgaataatc tgcttgcaca ttcgttgaat
    51001 tttgtcctct gacaatttcc agttcttctt cattcactaa ttgatactcc tctcctttct
    51061 tgaaatatag gacatcctgc atagaaaatc ctgcatcatt tgtgaaaggc tgagataatt
    51121 ttatcgttaa gatccacgga tttttcgttt ctcgctgatc ataaatccat aatttttcct
    51181 gtatatccat ttggccaaat gcttcttgtg tcaaaccagt tgctttgatc tgaccaaaat
    51241 cgagtttgga aggggcaaga aatgagagac caccttctac tacgatcttc gtagcaggtg
    51301 caaaaggcgg ggcatcatga taaacaactg gcgtatcact tggattgtga ttcccaattt
    51361 catcgttggt acttggcttg ttctgcacac ccttagcagt agcttctcct gcttggtcgt
    51421 ttaatacaat ttctaataca tctcctgcaa aaagttgatt ttctgggata gaaagaacaa
    51481 attcccctgt atcatcaata gttgtttctg acaaaacagc ttgatttttt ttcaatgaaa
    51541 ccaaggctcc tgcttcacct gtacctgtga tcgtgcgaga agctgcattg atcaatgtgg
    51601 tacttaaatt agcaggttca ggaggaatca catcgactgt cgttttggca gagtccagtt
    51661 ctgtcacttc ttggccacct cttacttttt tagcagcaac aacttgcact tgatcctccg
    51721 tttttaatct ctcatcattg ggtacttgaa cttcgaacaa gccgccttgt tcgccttctc
    51781 cccatgggtt atatcctgcc ccttcatttt catctgtttg tccttgcgtg agagcagtaa
    51841 tttctacaga tgatttcgtc gtatctgcgt aagtaatacg taaagtcact tctacttcgc
    51901 ctgtccaagc atctcttggc tttgcctcga caccttcggg aacaacgaca tgcccataga
    51961 tttttttatc tgcattcgtt ggcactctta aggattcaat catcgggagc tgattattgg
    52021 cactgattcg attataattt ttcattccgg cacctgtctg agcaaattca tttgttaaac
    52081 ctgcactagg gcttgatacg aaattgccta agtcggtccc attgatcgat aagtctgtca
    52141 tatcagaaaa ataatccggg tctttttcaa agttatcgaa tgttgcgcct gtttttcgcc
    52201 ataaagcgat ttctgaatgg gcgatcttta aagaagaatc tccatttcct tggaataccc
    52261 agccgcctat gccattggtc tcgcttccaa tccgtttgtt ttggaaatca aaataggtag
    52321 gagattctaa gtccacattc aacacatccc cactgatgat gccggtctta tcttcccctg
    52381 ttttcccgac catgactaaa taagtttttt cacctgccgt aaaatttaaa tcaatatctc
    52441 cagttgtatc tacagttgct ccggaatctg cgcgtatatc gatatttgat ttttctccag
    52501 tgactgtaaa atcagctgaa cctggacttt ctagaaaacc agaagcgtca taaaattgga
    52561 tcgcttgatt tccttgattg agtctgttca cgtaagattt gccatcacct gtatttctta
    52621 ccctcaatga gcctccattg gttactgtca gcttttgcgt accattctca aaacgaactg
    52681 cgctggaacg cccactctgc ttatcgatgt cgataactga tccggctcct tctacgatca
    52741 aagaaaggcc ttttcctaca aaacgcatcc cattgttccc gggttgatcg tcatctccag
    52801 ccattttgac cgagatttgt ccaccacttg atacatggat ttgactagaa tctccttgca
    52861 agcgaaatcc gcccgtagtt gctgtattga tggtgatttt cccaccattc gatacagtga
    52921 ctttcgatcc tgaacctctc atttggatga ttccgcgatt gttgtcattg gtaatattga
    52981 tctttgtatt taccgaaaat tcgcttcccg aaccactgac ttcaaacatg acattaccag
    53041 gtagcgtatc gactgcctct ccttcatcgc taacgccatc tgcccgatca taatcaagag
    53101 agacctttga accgcttaag atatggattc ctggcgctgt ttttccacta ccagtctcat
    53161 ctcctgaatc tagaaaaccg tagaatttcc ctagatacgg tccataaagt tgactaccat
    53221 tgatcacatt caattgatag cttctacaaa aataggcttt acccggagcg gcattcagtc
    53281 cttccttcac tggttggaca tccaacacac cggaaacacc atctaatgtg acagttgcac
    53341 gaggagcgta aatcagtcct aatttggacc catcatacaa atcaagtgtc ccggtaaatt
    53401 ttatttctcc cgtaaaatga tatctgcttc cccaaaaatc ggtcatataa gcaatgataa
    53461 tggcatttcc atcgatattt ggtgtcaatc cacctggatg tgcagctgtt cctttaaaat
    53521 tctggaactc gatcgactga ggtatctcat tgatataaat atggtgtgtg ccgaaattgt
    53581 gagcgaatcc accaaaatca ataacgatat ctcgtttgat ctctaattcc ctgtctaaag
    53641 tcaaaggctg aatcagtcgg atcttagtta ctgattcgtc tgcgagaagc tgcgccagtt
    53701 cttctgttgt ggcagcttcg ctgtacccat ccgctgaaac agctcttgcc agtgccgctt
    53761 cactgctatc agacgtttgt tcagctactt ctgaggtctc acttgtctta ttttcttctt
    53821 gccttttatt agtagaagcc tcactttgag attctgtagt ttcagaagta atcgattctt
    53881 ctgaaaaagt agtcttgtca aaactttgat tctctgattg cgttgattcg tttgttttct
    53941 ctacatcggc aaaaacggat gtcaccatca agagagggaa gacaaacaaa atcgacgcaa
    54001 tcagcttttt tacacattga ttcattctcc accatcctct ctgacgtttt gacaatttta
    54061 ttttatcgtt ttaatctatt tccaattgta ctttcatatt taaaatatgt atttttatgg
    54121 aaaaattttt ctattaggtg taaatttctt ttttgtacaa aaaaaaggct gtgacaaaag
    54181 tcttgtcaca gtcccttatc ccgaacaagc ggtgttctcc cgccaactcc tcggtcacaa
    54241 cctccatttt aacaataaac ctgtaaatgt gagggcaata ctttgattgt cacaggtaat
    54301 ttatcccctt catctccatc aacattcgtc gtcaattctt cctcatcagc taatgacaca
    54361 actaattgct cacaagtaag gtaggctaga tttttcgtag attcatctac tccttttaat
    54421 aggtctggca atgttttaag cgtatcgatc aaagaagaat cttttaaata aaccaaatgg
    54481 agcttaccat catccacttt tgcatttgga agtaatgttt caaaaccgcc gactgagtta
    54541 gttagtccta tcagcaaggt actgctctca atttcttcct tctttccatc aacttctacg
    54601 tgaaacgagt aggattgtgt ggaagctaac tgtttgattc ctgacatgaa ataagctagt
    54661 tttccaaatt tcgtcttctt ctccgcatct acatcattga ttgattcagg gatagagcca
    54721 attgcgacga cattcataaa atagtcgtca tttatttttc caatatccaa tggcttgact
    54781 gattcaatcg aaaaatgatt gatcgcttcc tcaggttcta gcggtatccc taatgctcga
    54841 gccaagtcat tcaccgtacc caatgggaaa aatccgaaat ttggccgatg ttcttgttca
    54901 gcgattccac tgattccttc gttgactgtt ccatctccgc ccatgacaaa cacactatga
    54961 tacccttccg tagccgcttc gcgagtaaag ttttttgcat cccctgcttt ttttgtatgc
    55021 aagacgacca cttcatcaaa caccgattcc aattttgcta tcgctaattg ctcaaattcc
    55081 tttgcttgtt caccgcctga actggggttt accactaata aaactttttt catcactttc
    55141 tttcccttct gattgatttc tttaatataa cgatagcata aagaaaaaaa ttaggctgtg
    55201 atgaagtact gttcatgaaa cattcattta ttttttgaca agataaaaca tccatactat
    55261 gaaactgagg aaaattaaaa caatgatata ggaaataaaa gaaaatgata ggcattgtat
    55321 acctaataaa gtaaaaatct taggtggcag ataaaaacac aaaaatagca agagaatgta
    55381 atagcgtttc ttccactttt tgttatcaga ccttatagcg atcagaggaa taccaaatgc
    55441 ataactgaaa aactcttgat gtactgttaa tatgtcacgg ccttgaataa taaatgcgac
    55501 aatccccgct ataattagtg tgcctaacac caaaacggaa aattgatgaa tatattttat
    55561 atacaaatga tccattgtac ttcctgcctt tttatttgtg agaaaaacaa atccgtatac
    55621 gtgaataact tctagaaata aaagaaattt gcaaaaccac tccatttatc tacttctacg
    55681 tgcatttttt gatattgttt tctttattta agattataca gaaaaatatc tagggtcgct
    55741 ggattctaag taaaaaaaat ttttaaaata aaaagaacta aatcaagtag taattaaact
    55801 actgttgatt tagttctaat aaaaagattt agatgaacag ttttattgat ttaaatagtt
    55861 catatttttt atttataccg cttatttact acttgctgta ctagaatctg aagtgaatgt
    55921 actcaaaaca ttttcaaagt ctttgtcttt gatttttaca ttagcgtctt tcaatacttc
    55981 gccaattact ttattctgga aggtactgtc acttaatttt gtgtctgttg caatttcttt
    56041 caattctttt ttgtatttat ccatgtcgtt gcctttgttt tggtttttca ccattttaac
    56101 cacataatat tccgtagtat aagttgaagc attggtagaa gtgatcacat cagaaacttg
    56161 accatctttc aacttaaagg ctgcttcttt gacttctgcc ggaaccgttg tagtagtgga
    56221 atcaaatttc acttttccgc catcttcttt tgtcgtagta tcagttgact tgtctttggc
    56281 taatttagag aaatcgtctc ccttatcagc agctttcttc acatcttttg catcatcttc
    56341 acttgctact tggataattt gagcttccac ttctggatgg aaagattccc aagccgtttt
    56401 taaatcttca tcggtcaatt tgatatgttt ctttagacct tcttgaaagg ctagactttg
    56461 tttgatactg tctttgaatg tttgttctgt gtaacctgca gatttcaatt gtgaatcaaa
    56521 tgaatcaccc aattgtttct ttgtttgatc atattgagaa tcgatttctt tggttgatac
    56581 gtcatctcca tatttatctt caaatacttg ataaacgatc aactgttgca cagcggaatc
    56641 tgctccttgc atttttttca agctatccat tacttcttgt tcgctgattg taccgccttt
    56701 ataactaacg gctgtttttt caccggcatt cgtacagcct gacaataagc cagtgcctag
    56761 tacgatggca agtgctaaaa atcttttttt catttatcaa ttctcctact tttcgttttt
    56821 tgcgaattgc taaaaacaaa ggtgttcaag atgaaaatca tcaagaacac ctttactaat
    56881 ctgctcgata cgtcctgaat agatataagg ctacacaatc cagaaagttc gctcagcaga
    56941 ataaatgtca agtagcacat cacaccttaa tatactactt cttttgttac cgtttttcaa
    57001 gtctatcttg cgttccgtat ttaacgtaac taattcttag aacaaatgtt atcgattttg
    57061 tttttcttct tccgctgctg ggatattcaa atcagacact tttatgttcg attttccaga
    57121 catttttttg ttgtttttat tatgatatat ttttatacaa ctaaaaatga atatattaaa
    57181 actaccaaaa ataactttta acgctgtttc attatatgtt ttttctattt tatataaact
    57241 tttggatact taataccaac cggttggatt gtaccaacat gagaatgtgt cagttctatt
    57301 tcctgtcgca cttgcatagg cccatactcc ttttgttgca tttgctgatc gaatagagtt
    57361 tccaactttc gcttttgcac cgtgacgatt atagacacga ctatagtggt tagaatgacc
    57421 tgtttttctc gtacaagttg tatcactata ataaacccat cttccagagc tatgattaat
    57481 tgctgttcta gaaatttccc ggtctactgg taaatttgac ttaataatat tacctttagc
    57541 atcaaaatga atctctcctg gctttaaaga tttgtaatag atttcaccgt gaactgaatt
    57601 tgcttccact tcagatgaca tagcgtttcc tccaacaaaa cctatcccta gtgcacaaat
    57661 aataactgct tttttcataa aacgacatcc tttccttttt gatttttaaa gcatgatctt
    57721 cttgtttcct attttggtca gatatggatc atgcgtgact acgataacta acgtaccttg
    57781 ggaagcttgt tgcgataaga tttcaactac tttatcctta tttcttttat ccaatgaccc
    57841 agtcggttca tcggctaaaa tgattttgct attcttcaat aagactctcg cacaggcgat
    57901 tctttgcttc tcaccaccag ataatgtgta gataggcgta cttagcttct tgtttatatt
    57961 tacttttttc aatgcctcat actttgctgt tcgcttatct gaaacattta aattcgaaaa
    58021 cttcaatcca atatctaaat taaaatcgat tgtttcatat tcaatcaacc caaaatcttg
    58081 aaaaagaaaa gatatggtat ttctttttat caagcgcttc tctctcccag ttaattgttt
    58141 cgtctcttga tcgaacaaat agaattgtcc tgaactgaac tgatcattca atcctaatat
    58201 attcaataac gtacttttac ctgctccact ttctccaaca acagtcacaa gatccccagc
    58261 atccgcagtg aaacgatcca aatgaagaat cgttttgttt cgaatgattt ttttcgcacc
    58321 ttttatcact atcaacggct ccaattgtta acctccattc atcagcaata actgactttt
    58381 cttttctgta gacaaaataa aaacaactga aagaataaaa tcaatgaagc taaagaaaaa
    58441 aatattaaaa agatatttag gctctctttc tataagaaaa aggaagatta cacttgtcgt
    58501 tccccatttg atgaggttca tcatgaagaa atctttatac gtttcaaaga gcgaataacc
    58561 acttgaacga aagatagcga ttttccgaca attgtagaca aaatatatgc ataacatgac
    58621 atagctcaac aagaagaaag aaaagaaagt gagtaaaaat atctgtatca agtaatgaaa
    58681 tgatctgctt aacactttga cagctatctc ttcagtctga gtatacggaa atgataactg
    58741 caagttatca cgtaactgac tttcttcaat cagctcaaat aacctttctt tcgacaaaga
    58801 gcctatcttg atctttaacg gtggatagat ttctccgctg agaatatttc tttccaaata
    58861 catactgttt ttcaatgtca gtatttcaac aaccggataa tgttcgatcc aggggataga
    58921 aggaataaat gtataaatgg gttgattgtt ttttataaat agaaatttcg gttctttttc
    58981 actgatttct tgataatatt ctgttatttg cgccaggtca ttttttaaat ctgtatgtct
    59041 atatcgttca gggatcaata taacagggtg cgtttctttt tcggttattt ggatcaacct
    59101 tccagagaca tcaatcaaag gaaatttctt aagataattc agatttattt ttatccctct
    59161 gccaaactgt tcattttctt cattcaagta atctgataca ttgactaata tacttccgtt
    59221 tttattcaaa gtttcatata attcctgatc tgctgctgat gcaaaacgat tggaataaat
    59281 aaaatccacg tggtttttcc cgatggttac tggataaaat acatgatagt cctcaccagc
    59341 gatttctttt ggtggttcat gggaaacagc taactgtgaa gaagaaaaaa cgatagttgc
    59401 taattcgaca ttggcaacgg tcaaaagaag agaaaacaac aatccaaatg taaaaagaat
    59461 gtattggata tagttgaacg cttctcttaa cgaagcgatt ttttcaacta ataaaagaga
    59521 aatcaaagag ataccatata ttccgctgaa caagaaaacg tacatctgga acaaataaag
    59581 aaattgctcc ttataataca taaaaataaa accaccaatg attcctgcaa ttatgaatgt
    59641 aataatgatt ttctttttcc ccaataaaca ccacaatact tgatgtaatg aaaagtcatt
    59701 attcaacaat aaactgattt ctttattcat caataagaaa aaggatgata acaaaaggaa
    59761 taaaagaatc aatccaatag ttaaatacac tcgataatta tatacgtctg tatttgaaat
    59821 aaccgaatac gaatagctat tttcaaagtc ttcaaaaata ttagtggcat aatcagtgtg
    59881 aaatgcttga ttgaatcgat cagtgaatag tgaaatgaac cgttcatagg cttctttttc
    59941 tctcgtcatc aagaaaaatt caccttcaaa atcttttatt ttgatcgatt cttcaatgga
    60001 cgttacgcga aaggaggtat tagaataggg taccttctcg tgagaagatt tgacatcccc
    60061 ttgataatac aatgtcattt caagctttgg ctgtatcttg aagttccaag atgtatctac
    60121 ttcattggat tcatttactg tttttttcat ataagaaagc tcagtttctg atgagacatt
    60181 ttcaaatacc atgattagct gctcatattc ttcttgccca ataaaatgtt gtggcaatcg
    60241 aatcggagta taagtatctg tttgatggaa aaattcattt gtccgataga gtacctgttt
    60301 atttatacta accaaggcaa aaccactcat gataccggtc actatgattg tgagtaaaac
    60361 tgtgaacttt tttttgatta tcaccactct cccttactta aatgattctt tgataaaatc
    60421 ataccgtttt tcatcaaaaa aacgtatcta tcaagtgtat aggtcatgta gaataaatct
    60481 atatactgta gaatcgaaag gagaagaaga tgaacaccgg agaaaaaata cgaacttacc
    60541 gcagaaattg taagcttacg caaaaagaat taggcgaaaa aattggtgtt tccgataaaa
    60601 cgatttctag ttgggaaaat tctcgtacaa tgccagacct tgaaatgctg tcacttttac
    60661 atcatacttt aggtctgccg attgattttc cttctattga atcagctacc gaatcaatag
    60721 aaccaacatt gccagatgaa cctcttgtta acaagaaatc ttattcatca aaacaaccct
    60781 tctcactcaa aaagacacta tctttctttc tgctcgtctt ttgtttggtc tatctttttc
    60841 ttatcaatta tcaatacaaa aatgaaacga tcgatcgatt caatcctttt ttgaaaccac
    60901 aaatcggcta taccacactc cctgtaggat acgtggacaa ttccatcgaa cgttgggtat
    60961 gtaatgatat ctttggccat ggtacatggc tgtcttttca cgctggagag gtagaaaaag
    61021 agaaaaacca tgctgtaatc aaacacaaag gaaaacacgt catgaacgtt cgcctgctca
    61081 cttgggaagc cattccctca gattaccaag aaatcctcag ccaagactat gacgacgcct
    61141 taaaaagaag ccctcaggag ccagataggc attaagtagg agatgaacgt agagatagaa
    61201 aaaatcaatg cacatgatct tatccaaaaa acaggggtac aaattaattg aaaaagcctt
    61261 gagatgaaag ttcctaaata aaaaagttca gaatgatagg tatttctggc ttatagtata
    61321 atatttacgc cacagcctcc caatgattct ttttggaatc ataatcaagg atggacattc
    61381 acaaaaatat tttgctcatt cgatccgaat agattaacga ccaatctagt aagtaatata
    61441 gattggtacg tagtcaagct gcacataatt tagatcaatt ttagttcttt tttatatttc
    61501 tatgatctgc tcatttactt ttatatgatg gcttacagta acataaataa atatgataat
    61561 cgctggtagt ttatccaaga gtactcaata aaatacaaaa tggattgttc aagcgcctat
    61621 cataatgata aaatgaaaca aataaacgat acaaggagga agagactttg agtaatggca
    61681 gaacaatttt ttcattgtaa aaattgttca gttgttaatc tatgggattt cggtagcatg
    61741 ggcatttttt tatagaggaa tcacaccaac ctggccgttt tatctcttgt tagtgggaat
    61801 attagtagga aattttgccc ctcttcacta tcgtacgaac ttttttccaa ctgattctgg
    61861 cctttttctt aaaacaaaga cgcgaaaatt agaaaacatt attgaaatga caattacctg
    61921 tataatcatt ttcttagtta tattcctaaa cgtcttctaa taagaatctc ttaaaattag
    61981 cttgttacag ttagttacct agtttatata acacagttat cttatcttcc ttatcaacat
    62041 cttttgccaa ctctttgtct taaattcgaa acatataagc agagctacga acaccaagac
    62101 tgtttcggct ctgttttttg catcaaattc actcttatat agctgataat ctcggctctt
    62161 taaaagaaat tattttcgct tcattttatt ataaaaaggc taggaacctt gatgttatca
    62221 ggttcctagc ctttatttaa gtattacccc acagaccctt ccatatagat ttttaaatta
    62281 cacactagtt taaactaaaa caaacaagga aaaacttacc atttaatcaa aaaattagct
    62341 catatgaata tattagaatg taaattagac tggtcaaaat ctggtcaaaa aagaggacga
    62401 atacaggtag tcctcttttt tgttattttg atattctaaa tcatttttta ttggattatt
    62461 cccttgttcg atatagaccc aaattgatct atcaataatt atcaaactga gtcatataga
    62521 gacaaaaatt tcaaaaatga cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg
    62581 acggagtcag acacggagtc aaaacaagcc cgctgagagt aaaaaatata cagtcccata
    62641 ggtaaagtgc cacaggactg tatatttttt tatttgagat tacctgaaat gattccattt
    62701 aatttttatc tcattttttc ctactagtta gtatcaaata ctctcctatt ttctcaataa
    62761 actattttgg gttacatggt atctgaaaca gctaaaacct tgtcattata gcaaaaaatc
    62821 actttatctc cgtttcattt cagatacatt tcacttaagc ggattccaaa aaaacaaaag
    62881 aaaaaggaac ccctagttag cctatgcttt ttattggttc ctctcaaaac acggtcatat
    62941 aacaacgggg tggggtaaat gaatgaaaaa ggtggaggaa acattattca atagctgtct
    63001 taaatacttc gttataaaat gtgaacaaat aaaagacatc tatccctatt ttctacactt
    63061 ccacttgact accatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
    63121 cattctcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
    63181 gtgggtattt aaatggcttc gcttctggat gtttggcttt atatccttcg taatccttac
    63241 tgttgataat tgctttcata ttaggaattg gatcttcacc tggtagatat tgcagattag
    63301 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaagacatga atcaaatcat
    63361 cactcgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
    63421 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
    63481 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
    63541 ttcctagtaa ttgatcaata taagttgaat caatatcata caacttagtg gtattttcac
    63601 tataaaattc gatcgttgaa aaatcttctc cttctttttc tggttcttcc tctattaatg
    63661 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
    63721 aagttggaga tttcaccatg tcatcattgt aatcgtcata agtcactaac gattcataag
    63781 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
    63841 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgt ttgaatcttt
    63901 ttttggattc ctgataactt ggataaatca aacctgattc ttcttttgct tttgaataca
    63961 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg cttacgaaac gtcacaatca
    64021 atccttttgt cttatcagga tacgtacgat tcgtacgtga aaaagcttgg atcaatcctg
    64081 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtta
    64141 atagacgatc cactacaatc gctaaatcga tttgtttccc aaattgtttg aattccgctc
    64201 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctaatgacc
    64261 aggaagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
    64321 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt ggaaaatcag
    64381 gatcctccat cgttcgtcct tcacgaatga gctgattggg aaactctttt gttaaccaat
    64441 caggctcttt cgtcatctct ttgatggccc gataatactt tttggccatg tcaatggaac
    64501 tcgtggttaa gattgcagat ttcgttggcc tgccatttcg aaaatcaaat tttgtatagg
    64561 cattatctgg acggaacatt ttatgaatga ctttttggat atgttcatct gcttcaaaaa
    64621 cagctgggtc caaataactt tcttttttga caggttccat ttgatcaatc atacgattga
    64681 tctgttctga tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
    64741 tctctagtga agtcggctcc acggtgtttt catgctctac ttgaaatcct aagacagagc
    64801 catcttctag cgcattctta atggtatagg tatgcaagac ctctccatac tgatcatggg
    64861 ttgtacgtgc caattgccct ttggcttgtt tccgattctc aggaaaaatc ggtgtacctg
    64921 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
    64981 t
//
LOCUS       DANJLF010000008        61757 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000008 DANJLF010000000
VERSION     DANJLF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="PWT25_000599"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="PWT25_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL1020706.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
     gene            complement(3004..3438)
                     /locus_tag="PWT25_000600"
     CDS             complement(3004..3438)
                     /locus_tag="PWT25_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020707.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3455..4660)
                     /locus_tag="PWT25_000601"
     CDS             complement(3455..4660)
                     /locus_tag="PWT25_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL1020708.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5354..5740
                     /locus_tag="PWT25_000602"
     CDS             5354..5740
                     /locus_tag="PWT25_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1020709.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5834..6025
                     /locus_tag="PWT25_000603"
     CDS             5834..6025
                     /locus_tag="PWT25_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020710.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6539..6871
                     /locus_tag="PWT25_000604"
     CDS             6539..6871
                     /locus_tag="PWT25_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1020711.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7167..7322
                     /locus_tag="PWT25_000605"
     CDS             7167..7322
                     /locus_tag="PWT25_000605"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL1020712.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7598..8938
                     /locus_tag="PWT25_000606"
                     /pseudo
     CDS             7598..8938
                     /locus_tag="PWT25_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9179..10795)
                     /locus_tag="PWT25_000607"
     CDS             complement(9179..10795)
                     /locus_tag="PWT25_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL1020713.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10913..11131
                     /locus_tag="PWT25_000608"
     CDS             10913..11131
                     /locus_tag="PWT25_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020714.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11326..11787
                     /locus_tag="PWT25_000609"
     CDS             11326..11787
                     /locus_tag="PWT25_000609"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1020715.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11980..12219)
                     /locus_tag="PWT25_000610"
     CDS             complement(11980..12219)
                     /locus_tag="PWT25_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020716.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12243..13766)
                     /locus_tag="PWT25_000611"
     CDS             complement(12243..13766)
                     /locus_tag="PWT25_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL1020717.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13784..13906)
                     /locus_tag="PWT25_000612"
     CDS             complement(13784..13906)
                     /locus_tag="PWT25_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL1020718.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13936..14919)
                     /locus_tag="PWT25_000613"
     CDS             complement(13936..14919)
                     /locus_tag="PWT25_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL1020719.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14943..15092)
                     /gene="rpmG"
                     /locus_tag="PWT25_000614"
     CDS             complement(14943..15092)
                     /gene="rpmG"
                     /locus_tag="PWT25_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL1020720.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15113..15490)
                     /locus_tag="PWT25_000615"
     CDS             complement(15113..15490)
                     /locus_tag="PWT25_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020721.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15522..15701)
                     /gene="rpmF"
                     /locus_tag="PWT25_000616"
     CDS             complement(15522..15701)
                     /gene="rpmF"
                     /locus_tag="PWT25_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL1020722.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15716..15985)
                     /gene="rpsN"
                     /locus_tag="PWT25_000617"
     CDS             complement(15716..15985)
                     /gene="rpsN"
                     /locus_tag="PWT25_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL1020723.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16508..18274)
                     /locus_tag="PWT25_000618"
     CDS             complement(16508..18274)
                     /locus_tag="PWT25_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020724.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18234..19987)
                     /locus_tag="PWT25_000619"
                     /pseudo
     CDS             complement(18234..19987)
                     /locus_tag="PWT25_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(20097..20666)
                     /locus_tag="PWT25_000620"
     CDS             complement(20097..20666)
                     /locus_tag="PWT25_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL1020725.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20684..22099)
                     /locus_tag="PWT25_000621"
     CDS             complement(20684..22099)
                     /locus_tag="PWT25_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1020726.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(22110..22778)
                     /locus_tag="PWT25_000622"
     CDS             complement(22110..22778)
                     /locus_tag="PWT25_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL1020727.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22908..23492)
                     /locus_tag="PWT25_000623"
     CDS             complement(22908..23492)
                     /locus_tag="PWT25_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1020728.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23823..24056)
                     /locus_tag="PWT25_000624"
     CDS             complement(23823..24056)
                     /locus_tag="PWT25_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL1020729.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24071..25021)
                     /locus_tag="PWT25_000625"
     CDS             complement(24071..25021)
                     /locus_tag="PWT25_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL1020730.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(25018..25860)
                     /locus_tag="PWT25_000626"
     CDS             complement(25018..25860)
                     /locus_tag="PWT25_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1020731.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25862..26581)
                     /locus_tag="PWT25_000627"
     CDS             complement(25862..26581)
                     /locus_tag="PWT25_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL1020732.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27273..27431
                     /locus_tag="PWT25_000628"
     CDS             27273..27431
                     /locus_tag="PWT25_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1020733.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27503..28729
                     /locus_tag="PWT25_000629"
     CDS             27503..28729
                     /locus_tag="PWT25_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1020734.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28817..29209)
                     /gene="rpsI"
                     /locus_tag="PWT25_000630"
     CDS             complement(28817..29209)
                     /gene="rpsI"
                     /locus_tag="PWT25_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL1020735.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29225..29668)
                     /gene="rplM"
                     /locus_tag="PWT25_000631"
     CDS             complement(29225..29668)
                     /gene="rplM"
                     /locus_tag="PWT25_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL1020736.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30187..30654)
                     /locus_tag="PWT25_000632"
     CDS             complement(30187..30654)
                     /locus_tag="PWT25_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL1020737.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30774..32312)
                     /locus_tag="PWT25_000633"
     CDS             complement(30774..32312)
                     /locus_tag="PWT25_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL1020738.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32392..33078
                     /locus_tag="PWT25_000634"
     CDS             32392..33078
                     /locus_tag="PWT25_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL1020739.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33279..33806)
                     /gene="msrA"
                     /locus_tag="PWT25_000635"
     CDS             complement(33279..33806)
                     /gene="msrA"
                     /locus_tag="PWT25_000635"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL1020740.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33890..34567)
                     /gene="pgmB"
                     /locus_tag="PWT25_000636"
     CDS             complement(33890..34567)
                     /gene="pgmB"
                     /locus_tag="PWT25_000636"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL1020741.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34564..35694)
                     /locus_tag="PWT25_000637"
     CDS             complement(34564..35694)
                     /locus_tag="PWT25_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL1020742.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35694..37877)
                     /locus_tag="PWT25_000638"
     CDS             complement(35694..37877)
                     /locus_tag="PWT25_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL1020743.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37874..38899)
                     /locus_tag="PWT25_000639"
     CDS             complement(37874..38899)
                     /locus_tag="PWT25_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1020744.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38944..39771)
                     /locus_tag="PWT25_000640"
     CDS             complement(38944..39771)
                     /locus_tag="PWT25_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL1020745.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39768..40835)
                     /locus_tag="PWT25_000641"
     CDS             complement(39768..40835)
                     /locus_tag="PWT25_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL1020746.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40861..41697)
                     /locus_tag="PWT25_000642"
     CDS             complement(40861..41697)
                     /locus_tag="PWT25_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1020747.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41694..42563)
                     /locus_tag="PWT25_000643"
     CDS             complement(41694..42563)
                     /locus_tag="PWT25_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1020748.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42633..43916)
                     /locus_tag="PWT25_000644"
     CDS             complement(42633..43916)
                     /locus_tag="PWT25_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL1020749.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43930..45615)
                     /locus_tag="PWT25_000645"
     CDS             complement(43930..45615)
                     /locus_tag="PWT25_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL1020750.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45890..46915
                     /locus_tag="PWT25_000646"
     CDS             45890..46915
                     /locus_tag="PWT25_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1020751.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47510..51184)
                     /gene="rpoC"
                     /locus_tag="PWT25_000647"
     CDS             complement(47510..51184)
                     /gene="rpoC"
                     /locus_tag="PWT25_000647"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL1020752.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(51242..54859)
                     /gene="rpoB"
                     /locus_tag="PWT25_000648"
     CDS             complement(51242..54859)
                     /gene="rpoB"
                     /locus_tag="PWT25_000648"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL1020753.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55587..56555
                     /locus_tag="PWT25_000649"
     CDS             55587..56555
                     /locus_tag="PWT25_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1020754.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56573..57478
                     /locus_tag="PWT25_000650"
     CDS             56573..57478
                     /locus_tag="PWT25_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020755.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57475..58281
                     /locus_tag="PWT25_000651"
     CDS             57475..58281
                     /locus_tag="PWT25_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1020756.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58417..59406
                     /locus_tag="PWT25_000652"
     CDS             58417..59406
                     /locus_tag="PWT25_000652"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL1020757.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59723..60247)
                     /locus_tag="PWT25_000653"
     CDS             complement(59723..60247)
                     /locus_tag="PWT25_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL1020758.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60534..>61757
                     /gene="scm"
                     /locus_tag="PWT25_000654"
     CDS             60534..>61757
                     /gene="scm"
                     /locus_tag="PWT25_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL1020759.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDS"
BASE COUNT        18739 a        12411 c        10340 g        20267 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2581 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2641 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2701 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2761 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2821 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2881 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2941 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     3001 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3061 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3121 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3181 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3241 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3301 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3361 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3421 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3481 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3541 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3601 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3661 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3721 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3781 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3841 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3901 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3961 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     4021 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4081 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4141 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4201 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4261 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4321 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4381 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4441 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4501 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4561 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4621 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4681 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4741 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4801 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4861 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4921 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4981 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     5041 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5101 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5161 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5221 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5281 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5341 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5401 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5461 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5521 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5581 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5641 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5701 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5761 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5821 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5881 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5941 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     6001 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6061 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6121 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6181 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6241 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6301 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6361 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6421 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6481 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6541 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6601 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6661 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6721 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6781 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6841 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6901 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6961 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     7021 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7081 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7141 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7201 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7261 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7321 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7381 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7441 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7501 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7561 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7621 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7681 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7741 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7801 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7861 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7921 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7981 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     8041 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8101 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8161 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8221 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8281 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8341 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8401 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8461 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8521 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8581 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8641 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8701 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8761 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8821 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8881 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8941 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     9001 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9061 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9181 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9421 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9481 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9601 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9781 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9841 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9901 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9961 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
    10021 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10081 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10141 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10201 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10261 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10321 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10381 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10441 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10501 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10561 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10621 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10681 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10741 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10801 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10861 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10921 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10981 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    11041 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11101 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11161 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11221 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11281 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11341 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11401 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11461 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11521 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11581 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11641 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11701 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11761 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11821 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11881 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11941 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    12001 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12061 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12121 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12181 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12241 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12301 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12361 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12421 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12481 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12541 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12601 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12661 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12721 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12781 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12841 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12901 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12961 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    13021 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13081 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13141 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13201 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13261 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13321 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13381 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13441 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13501 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13561 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13621 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13681 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13741 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13801 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13861 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13921 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13981 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    14041 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14101 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14161 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14221 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14281 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14341 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14401 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14461 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14521 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14581 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14641 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14701 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14761 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14821 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14881 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14941 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    15001 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15061 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15121 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15181 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15241 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15301 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15361 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15421 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15481 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15541 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15601 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15661 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15721 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15781 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15841 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15901 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15961 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    16021 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16081 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16141 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16201 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16261 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16321 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16381 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    16441 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    16501 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    16561 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    16621 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    16681 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    16741 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    16801 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    16861 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    16921 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    16981 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    17041 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    17101 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    17161 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    17221 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    17281 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
    17341 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    17401 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    17461 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    17521 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    17581 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    17641 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
    17701 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    17761 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    17821 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    17881 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    17941 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    18001 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    18061 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    18121 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    18181 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    18241 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    18301 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    18361 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    18421 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    18481 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    18541 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    18601 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    18661 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    18721 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    18781 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    18841 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    18901 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    18961 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    19021 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    19081 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    19141 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    19201 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    19261 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    19321 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    19381 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    19441 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    19501 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    19561 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    19621 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    19681 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    19741 aggatatgag aaaataagcc agaaaaaata taaaataaag ctctaaagtt aaccaagcaa
    19801 cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa
    19861 gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata
    19921 aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat tttttgtata
    19981 ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg
    20041 ctttccgtaa tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat
    20101 tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct
    20161 acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt
    20221 gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat
    20281 ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga
    20341 ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta
    20401 atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa
    20461 acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat
    20521 atgattccag ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca
    20581 atactactaa ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca
    20641 ataaaaatat aatcttttgt tttcatatat attctcctca attctatttc gttatgtcat
    20701 taaaaatctg ttctatcaaa gttgaatctt ctactctaag ttttatatct ttttttattc
    20761 caccattttt taaataaata attctattgc atgttttata aagaaattcc ggatcatgag
    20821 tagctataac tactgcaccc ttttctttta agtctttaat aaggtcagca atcctttcca
    20881 tattaatata atccaaccca cttgtaggtt catcaaatag aaataaatta gtttttgaca
    20941 atgaagctat agaaaccaat aatctttgtt tttctccacc ggataaatca aacggtaatt
    21001 gatctttcat tttataaagt tccatttttt ttaaaatttt ttcttgctga tctttactaa
    21061 ctatttcatt ccccagggct aactcgctcc tcactgattc tgtgaaaaat tggtaatcca
    21121 tttcttgcat tactaaaaac ggcgatgaca aatttttttt attttcgatt atctttcctt
    21181 tggtagggcg aataatcttc atgagtattc ttagtaaggt tgttttcccc acaccgtttt
    21241 tccctaccaa accaataaca tctccaactt tgacatcaaa atttatgtct tttagaatat
    21301 tttcataagt aatttcttca acttttaata catctgcact taccttttcg atatagggaa
    21361 cttcaatcga actattaaaa atgtcaaatt tccttaatgc gttattgttt tcagttttta
    21421 acaactgaat agttgtagtt tttacagttc tatttttaac aaatagaact tgatcaatta
    21481 attctttcaa atagtaaaaa cgatgttcag ctataacaat agtaaatcct ttatttttta
    21541 aatcacaaag taagtcagca agtttcttag tcgctttgaa gtccaaattt gaagacggtt
    21601 catccaatat aattaattta gagtttagcg ataaactaca tccaatagaa attttctgcc
    21661 gttcaccact tgatagcgaa aaaatgtttt tatctaatat atttgaaaga tctaataatt
    21721 cagctaattc attgattctt ttatccatat gctcttttgt aaatccaaag ttttccatcg
    21781 aaaatactaa ctcactcgtt gtatttgttg taaagaattg tcctctagga tcttgactta
    21841 cgtagccaat attttttgat atactttctt gtgttaagga ttgtagcgag ttattttcaa
    21901 ataaaatttc cccaaccaat tttccgtcat aaagtcttgg aataagtcca ttcattaact
    21961 ttaatagtgt ggatttccca ctcccagaag aaccagtaat caatgtaaga ctgccttttt
    22021 ttattgaaaa attaaggtca acaagcgctt gttccttaac atccttatat gaaaaagaaa
    22081 cattgttaaa ttcaaacaat tctttcacct caaatcaaaa tacttaagaa aaaaatagta
    22141 acggaaaaca ggatgatata gtctaataag ctcatgtttg ttttatagta agaacttctt
    22201 ttcacaggag attctatccc ctttgttatt gcattagcgg tcatttcttc agcaatcata
    22261 gttgcacgta taagaacggg aactaacaga aattccaaat attgcagagg ctgaaaaaaa
    22321 gacaagcgaa tgtttctcat ttttaaagat tctttaatca ttcttatttc agaacgataa
    22381 gtcggaaaga atctgatcac tactgtaata cttaacatta tttttttcgg taaatgtatt
    22441 ttttctaaag aggataataa ttcattgcta gtatagacac taaccaacaa ccatgagatc
    22501 attacgaccg gcaacattcg aaatacaata tataaggatg tcgcaaaaaa tatagtaaaa
    22561 aactctatct tgatagcatc aataattttc attactaaaa acagaactga ataaatcaag
    22621 atgaaataaa tacatgtctt tattttacga aacatcaata ctaaaactgt agcaataatg
    22681 aaacacccca cttgaaatat atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata
    22741 tttaaagtaa tcaaggttaa cggattgatt tttgacactc tttcacctcc aaaaaataag
    22801 atcgcactgt tatcttaagc tgataaaaat gcaccgttat tcagataaca gtgcgatctt
    22861 atcaaaaagc ataactgaga acaattctca atgcaagctt tttttaatta actcagtaat
    22921 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
    22981 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
    23041 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
    23101 tctagacaac agactgtctt tttcgtcaca aatgatgtaa ttttttcaac aatatcttga
    23161 tatttggttc cagctgattg gaataaaatc aaatcgaata tacgtttatt ctcaaaaata
    23221 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
    23281 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
    23341 atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa
    23401 gcacccgtag ttacgcctgc ggtcaggcac agttgcctta tcttagtttg ctgaaatcct
    23461 tctttttcaa aatggcttag tccactttct attaactttt ggtgagttat aatataatct
    23521 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctttattatc ttacacagga
    23581 cttgaagttt caatcgttaa cgtttattac tgaactcatt ccttagagct aaactaatga
    23641 gaaagagcag aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
    23701 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
    23761 gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagtc
    23821 ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga
    23881 aatcctatat ctaaatagtc ctcatatcta agtttagcca gcatctcact tacagatgct
    23941 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
    24001 aattcctgaa ttgctagtaa ataaattagt atagtagtcg ttttaatttg taccatacct
    24061 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
    24121 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
    24181 agataggaat attggtatct tttgatactg ttttcattgg tctatcatcc acactgcttt
    24241 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
    24301 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
    24361 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
    24421 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
    24481 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
    24541 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgagga tcttccttcc
    24601 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
    24661 ctttgttttc ctctttgttc gcatttttaa ctaattttgt aaaccaagca tttcctccag
    24721 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
    24781 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
    24841 ttttatcttt tgctatattt tcggtaatat ctgctaaaat cgaattagta actactactt
    24901 ttaatttttc gttcgaagcc actttatctt tgtctttact atttattgaa catgccccga
    24961 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
    25021 taacgatctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
    25081 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
    25141 aataaacaat ccaagcaatg atgaacttgc tccgactgtt gcagaaataa atatcatagt
    25201 tttcaagcta tttgcatata aatatgctgt agctgctggt gtaattaaca ttgcaacaat
    25261 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaaccatcaa
    25321 tagataatga taaaaactga cattcatccc tattgctttt gccattaatg gatcaaaaga
    25381 agtgattaat aattctttaa aaaatagaac tactataacc aacactaaaa ccgaaacgcc
    25441 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata aaatatgaaa
    25501 taaatccgtt gagctatttg caactcctat aaggataact cctagtgcta aaaaagaact
    25561 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
    25621 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
    25681 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
    25741 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
    25801 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
    25861 attattcccc ctctccaata aatatagtat ctccataggc tttttttaaa ttttcttcat
    25921 taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga ataatattat
    25981 caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactgtt tttccctgat
    26041 gtttcaattt ttttaacgta tccataatta ttcgttcact aactaagtct atccctacaa
    26101 atggttcatc taaaaatatg aaatccgcat tttgagcaag acatcttgct aaaagtacac
    26161 gttgaaattg tcctccagat aattctccta tttgatgatt agaatatttt cccatatcga
    26221 ccttttccaa agcctttgaa actctttgcc attctgcatt tttaattctt tgaaaaactt
    26281 tcattccggc atatgttccc atagatacac attcttttac ttttattgga aaagtaaaat
    26341 caatatcagc tttttgctct acatatgcaa tcttcaccag ttttttattg atatcttcat
    26401 tattaatgga gacctctccc tgatgaggga taatatttaa cattgcttta agcaatgtgg
    26461 attttccagc accatttgga ccaataatac ctgttaaagc accttcttgt atttttaaag
    26521 aaatctcgtt caatgctaaa aaatcgttat aacaaacatt aagctttttc acttctaaca
    26581 taactttctc cttctaaatg ataatgatta tcacgatcat atggtataca aaaattatat
    26641 tttagtcaac cctaaatttt atagtttaga tttttttctg ttgctgaaga ttagaatgac
    26701 aaaactattt gaaactacgc gtatagtaag tagctttcaa aatatataaa aaaggagcgt
    26761 tctcaaatga gaacatgctc ctaaaactac tttattggcg tatggaaaaa ttatacttga
    26821 agtggatagc gggttgaata agttgataag catcaaaaca tttataaaaa ttattcaaga
    26881 ctatgtctgt tccatataca aatatgaaaa tcagtaaact ccatttaact tttttcgtag
    26941 tgtgtctttt tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa ctatttttga
    27001 tcattgacaa ttaaatacaa tttttgaata acgatccctg ctattcgagc tagaaactca
    27061 agtacgccat gacctgatta gcgacaaata cttagagatt tctttttgtt ctgggaaaac
    27121 aaaatgccat gacaatagca gcaaataatg atacttccgt aaggctcgcc gtaaactggg
    27181 gagaggtgaa attcctatgt gcttacccaa agcaattcaa aaatttcata actactattt
    27241 taggagatga ttttatagaa tccagagtct acctgaataa aaaacaaacc tgtgcatttc
    27301 taggaatcga taataatact ctagataagt ggattcgtat atataatttc ccagtaataa
    27361 gagttggtgg tgtagttcgc tttgacgtag aagatattaa agactttatg aatacattta
    27421 aaataacttg aaattttctt ctcgccgcat atactaaaat tagtgaaata tactgcctta
    27481 ctttttttaa ggagtgagga ttttgcctac aaagctttca aatgggaaat ataaaactaa
    27541 tctgagatat ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa agtatcaaaa
    27601 aacatttcca aataggcaat tggcgattaa agccgaaaat gatatgaaga agaaaataga
    27661 aaaagttttg agagaggaga atgcaaactc attagaatta aaaggaaaaa taacgtttaa
    27721 aaaattttac gaatctaaat ggcttcctag atatgaatta ggtcaaacaa ttagatcaaa
    27781 caggcctcct agtgatataa ccattagtaa tacaaaagat atatttcggt tgcatatttt
    27841 acctatgttt ggtgaatacg caatgaatta tctaaatatc aatactgaga taatatcaga
    27901 tgaattaacc aaaaaatcga aagaatatgc aaatataaaa atcataaaag gatatgttcg
    27961 ttccatgttc gatattgcag aaatactaaa ttatatcgaa tttaatcgaa ctactaaaat
    28021 tattcaaagt atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa tacaagaagg
    28081 taagcaagca ctatcatcaa aagaacttac taattggata gaagcagtaa atgatgattt
    28141 taataatcat ttacttactt ttcatgatta cacacttttt atgattaccc tgtatcttgg
    28201 tgatagaaaa agtgagactt acgctttgca atggaaatat attgattttg aaaaacaaac
    28261 ggtccgtctt aaacatactt tagataaata tcaaagaaaa aagtttacaa aaggtcgaaa
    28321 agataccgtc attcaagtac cagaagttgt aatgacattg ctaagtgaat ggaaatcggt
    28381 acaagctgat caattgttga aattaaaaat taagcaaacg ttagatcagt atctctttac
    28441 ttatactaaa ccatctggag aagtaaattg tcctgttcat gccgattacc taaactatcg
    28501 aatcaattca attaaaagaa ggcatcctga cttagttcat ttatctccac acaaactccg
    28561 tcatacttat gcaactatag ctagacaagg tggagcagat atgaatcaaa tttcaaatgc
    28621 tttaacacac tcagatattt ctacaacaaa gatatatgtg aacacaccag atatagttga
    28681 taaggctgtt tttgaagctt ttcagagagg attgaataag tgtgattgat atgtgttctt
    28741 aaatgtgttc ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg ccagaaacgt
    28801 tgatatataa aggatattaa cgttttgaga attgtgaagc tttacgtgct ttcttaagac
    28861 ctggtttttt acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag
    28921 ctaaacggaa gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc
    28981 ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg
    29041 tttcagtaac agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt
    29101 cttcaacatc ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag
    29161 caactgcgtt tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct
    29221 cctattagat taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt
    29281 ctgctccacc gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa
    29341 gcataccttt tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag
    29401 cagtgattga tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg
    29461 cttttttacc tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat
    29521 ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg
    29581 aaagacgtcc caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc
    29641 ctggtttggc catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt
    29701 gacatacact tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta
    29761 cccgctaatc tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt
    29821 tacttctttt ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag
    29881 aggctgtgac aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca
    29941 actcctcggt cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt
    30001 tcttgtgact cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag
    30061 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt
    30121 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa
    30181 cagtttttac gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg
    30241 tttttctgtg gctgttttga attcgataaa gatatcttgt gttggatcgt cgccttcttc
    30301 tccagaaact tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag
    30361 tgtttccaaa cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt
    30421 gttatctgca aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc
    30481 ggatacttca tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa
    30541 tgtaaggaaa cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg
    30601 gcttagatct gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct
    30661 tgcctcctaa cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca
    30721 aaatcattta cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt
    30781 tcagaatcag ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc
    30841 ttttgtgtcg aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt
    30901 actccaactt gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt
    30961 ccgatgcttc tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg
    31021 aaaacaagcg ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca
    31081 aacccgcctg tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc
    31141 cgataaaggt tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt
    31201 tgccgctgta aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca
    31261 ccaagtaaat agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc
    31321 gaaagcagtg catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt
    31381 aaaaatgtat gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact
    31441 acccaatgtt tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc
    31501 tgagtagtaa taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca
    31561 ccaaaagcac ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat
    31621 atatcatttg gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag
    31681 gtggaaggaa agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct
    31741 tctgcccatt ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg
    31801 ctgatcagtt cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca
    31861 gtttttttga ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc
    31921 attggatgaa tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc
    31981 agtttttctg cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt
    32041 gttttctctt catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga
    32101 atgattgtgt ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga
    32161 aagatcaatt cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga
    32221 aaataagcgg atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg
    32281 gttccgatat ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac
    32341 aaaaatttag tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt
    32401 tattttatca ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg
    32461 ttaggtcact ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct
    32521 tggtatgaat tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc
    32581 aggtgtcggt tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc
    32641 tggttcttta ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct
    32701 ttgctatttt cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt
    32761 tcttgggcaa ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg
    32821 ctgttaacgt ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct
    32881 ggcgatactc tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag
    32941 atcttgtttc ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat
    33001 aaaaaaaagt tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc
    33061 cttcatctta ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat
    33121 agtgttctct taccaaatcc ttgtgacttg gagctaggcg acaggttcac aacctctcca
    33181 ctaaatggtg ttcaatcagc tgaccctcag tattagctca aaatgctggt tttacaacct
    33241 ctgatatacg gtgttccata agtagctttt tcggcttctt atttcttgac gccccaatgt
    33301 cgacgaagga aacctgcacg tccagaacct tctcgataaa gattgtaatg gagcttgttt
    33361 ttcttgtaat aatcctgatg gtaatcttct gcaggataaa attcgcttct tggttcgatt
    33421 tttgtcacga ttggcttatc gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct
    33481 gcaatatttc gttcttcttc atcattatag tagatcactg gacgataaga atcaccgcga
    33541 tcagcgaact gtcctaatgc gtcagtcgga tcggtttgct gccaatagat ttccaccaac
    33601 tgatcataag aaataacttc tggatcatat tcgatctcta ctgcttctgt atgccctgtt
    33661 gtgcctgttg ttacttgttc ataagttgga tttggtacat gcccgccagt ataaccggat
    33721 gtcacagaaa tgatccctgg ctgctcttca aatggtttga ccatacacca aaagcatccg
    33781 cctgcgaaaa ctgctttttt tgtcatataa aatccccctt ttttctcatc ataccttgat
    33841 ttccaaaagg aggataacgt tttgcttgtg acagaaaaaa cggaaactat tatatacgtg
    33901 atacggcttc tgataaatgc caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa
    33961 tcacatattc agcagaagga agatttatgt tttcacctat tcccaataca tgcatgttac
    34021 cagccaaagc tgcttgacaa ccagcttctg aatcttcaat gaccaagcat gcatttggcg
    34081 gtacattcaa ttgctgtgct cctttgagaa aaacttctgg atcaggtttt gcttttgata
    34141 cttgcgtgcc gtcaataagg acatcaaaat aacttgtcaa attcgttttt tctaaaatga
    34201 ggcgtgcatt tttgcttgct gatcccaatc cgatcttgat gtttgttttt ttcaagtact
    34261 caagaacagc tattgcacca ggcaaaacgg atgtttcatc cattttttga atcgcttgaa
    34321 gatatagatt gtttttttgc tcagcaaagg ctgatttctc tttttctgag aatttctgtt
    34381 ccatttttcc aaatgacagc aatcgctcta gtgactcggt tctggatatt cctttcagtt
    34441 gctcgttttc tttctcagtg aatggaatgg acagctggtt agctaaaacc agccacgctt
    34501 cgtaatgata acgagctgtg tctacaagaa cgccatctaa atcgaatagt gctgctttga
    34561 ttttcatcga ttacctcctt gaaaatgaac gacttgaagt aaagatgcga tgtttttagc
    34621 tgtgcgatga agattatcag tggcttgctg taaagcttct tgtaaaggca cagctgcagg
    34681 aaggatcgga aaaacggccg aaatgccaac atctgctgct ttttcgcttc ctttaccaac
    34741 acttccgcaa atagctatca caggaacgcc ctctggtact cttctagcaa tcccaactgg
    34801 cgcttttcct tttagacttt gtccatctag acgtccttcg cctacgatta cgaggtctgc
    34861 ttgctgtacc cgtttatcga actccagtaa atccaatacg tagtcgatgc cagagactat
    34921 tttccctgaa gcaaatgcta acagtcctgc acccattcca ccaccagcac tactgcccga
    34981 tgcctcgatt acttgagggg caaatctcat ataaaaatca tgcatggctt ggtcagccac
    35041 tgctaattca ttttctttca agcctttttg tggtccgaaa acaaaagaag cgccttcttt
    35101 tccgcataaa agattagtaa catcagtaat gacagtgatt ttggctttaa tcagctcaat
    35161 aggcacgttc gttttatcaa ctgtagcaat tttacctaga ttttctccca gtgcttctac
    35221 tttttgatta tcttgatcgt aaaaagcata acctagacct tctgccatgc cgatgccgcc
    35281 atcgttggta ctacttccgc caacgccgat gatgatgtgt cgaacacctt gttgcaacaa
    35341 ttgtcgtatc agttctccta ctcctcgcgt ggataaccta agtggctgtt gttggtttag
    35401 tgggacatct ttcaatccga cgatttctgc catttcaaaa atcgcagttt gaccgtctga
    35461 agcgtactgg atttctttgt ttttgccaaa tgcattagta acgactgctg tttgccgctt
    35521 caaccttaaa ctgtctgcta atgatgaaac cgttccttct cctccatcgc ctataggtag
    35581 cagatcatat tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc
    35641 ctctaaggct gtcagacttt ctttgaaaga atctggcgca atcagaatac gcattatttt
    35701 cccctccgtt ccaagatcag ttctttttcc agctggtatt ggtctccatt gacaaaaata
    35761 ggtaatgttt ctttcgtctc tttttttatt actatttgtt tttttgtcaa aacaaatagc
    35821 aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt cccatgctga aggaagtctt
    35881 ggagaaatag aaagagttcc attcgttact gtaatacctg aaaaaccaaa tataactgct
    35941 agccatagtg ctcctaatga agcagcatga attccgtcat ctgatgaatg cggatctggt
    36001 cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg cccattccat ttcttggata
    36061 cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat tgttttgttt
    36121 tcatagtaat gcaaattctt agaaacgatt ggttgagaaa aatactcagg taataagtat
    36181 aacagcatga caagatctgc ttgcttgctg acttggagtt catttacttc gctacgggca
    36241 taatcaagta aaatagactg gctgccagga cgttctttgt aagtggtcaa atcaataact
    36301 ggcttactga gatacgtgtc atcttgcgga atcagctgtt ctttgttagg cactggcaaa
    36361 tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat
    36421 ttaacagcta aggccacatt ataatgcgcc agatagttcg tatatgcatt attgtcgata
    36481 tattctgtgt attcatctgg accaatgact ctgtgtatct ctaaatgatc atcgactttt
    36541 acagctctgc tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc ctttgtttct
    36601 aaaaacgatt gatcctctgt tgcttggaca tactcttgaa cggcataagc aaggtctgca
    36661 ctgatatgat gttctgcggc agctgaagca acttcttgtc ttgttccggt tttgatattc
    36721 aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt
    36781 gcaccttgat agtgataatc tcttgctttt tgccgtgcgc cttctaatcg atcataacgg
    36841 tacagcagca aattctttgc tacttcagga aactgatata aaaagaaagg caggataaaa
    36901 atttccgtat cccagaaaac atgcccttta taaccttcgc cagttagtcc cttcgcccct
    36961 acactaacac ttggatcatt gcggggagtc ataatctgta aatggtaagc agcgaagtcc
    37021 atcgctaact gatcgaaaga agaatcgcct tggatcgtta tccgatgctg atgccaaaaa
    37081 tctgtccaat aagacatcga ctgctctaaa atttctgtat aactaacgct tttctgggct
    37141 tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag aggtagagag cacgatgatc
    37201 ttctccagcg taaaggacgt attttttctg atcttttctg aaaaggaagc ggtgatccgg
    37261 cgattttttg cttggaatac acctgcttta ttgaatttag ctgcaatccc aatatcaatt
    37321 ttactttctg ttgtctggta ggatgcactg atcaaatcgt caccaaatac tcgcagttct
    37381 ttttcaacca actgctgtgt accaaaattg gtttgctgtc catcgatacc cgttttcaca
    37441 tctacttggc agtcttcggt caacggcttg atgaccactt tcagggctgc ctgatggaga
    37501 ttattttgat cagcaacacg acaagtaatg acttccactt tttttccttg agcagtttca
    37561 atgagcgcat agcgtttaag ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga
    37621 actttcatcg tctcaagaga aaaatgttca ccgtcgattt ttatttccca tctgatgaca
    37681 tctgggagat tcactaactc ggctgattct gctcctaaag gtcggttata tacaccagct
    37741 gcaaacattc cccttacttg attccgatag tcttcctcct ggcttcctct gataccaaaa
    37801 aatccatttc caatggtcat ctgactcgca tacttattga tatactctaa atcaaactgt
    37861 ttttcttcta ctttcataag gtaataactt ctcctgtttc tgccgaacga tataaatttt
    37921 ccaccagatt ctggatgaca tatccttgcc agccatctgc aatcatctga gtctggtcac
    37981 cgagcgcccg attcacaaaa tgagccatac ttagttcatg tgggtcatta actggcgtgg
    38041 tcttttgtaa ggaaaccagc tttccatcta catcttcgaa tagttcgcct ggatacagat
    38101 tagcacctgc ctgatcgcca caaaaagcca cattcagttt gtcttctctg atattcaaag
    38161 caaacgaagt attcagttgg atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg
    38221 catcttctac tgtgaatttg cttggatccc atttcccgaa tttccctgaa ttttttttcg
    38281 taccaatttt ttggaagctg tacgcccgaa cgctttttac tttaggaaat ccagtaatat
    38341 acatcgctgt atcaagcatg tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt
    38401 ctttgtcgat aaacgttccc cagcctggaa caccagaccg acgaagcgca ttcgcttcta
    38461 cgtaataaat atccccgaga acatcaagat gattcttgat cattgttgct tcgtcagaaa
    38521 aacgatgctg gaatccataa gccaaaatct gcccgttttt atcggctgtc tctgccatct
    38581 ttcttgcttg atcagctgtc atagctggtg gtttctcgca catcactgaa gcattagctt
    38641 cgagggcagc caatacatgt tcgaagtgaa agcgattggg tgtacagaca ctcacgatat
    38701 cgggagaaat ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac
    38761 gtcggcaaaa gtcttctgct cgctggctga ctggatcaac cacagcggcc aattctaaga
    38821 catctgatct tttttgataa taagcagcat gtactttttc tgctacttgt ccagcgccta
    38881 tgattgctac tttcttcatt tatttctctc tcctttgctt atttacttat tgttattctt
    38941 tctctattct ttctctaaat catttaaatg acgcgctgtt tccagacagt ttttcaagta
    39001 ttgcaatgat tcctgataaa ttttcacttc attttctcct cgcaaacgac attcatatac
    39061 catataacct tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc
    39121 gccagatcct ggttggtagc gatgattatc tgctaaatgg atatgaccca catactcttt
    39181 ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata ttcatatgat aaaaatcagc
    39241 gatgatctgc acatgtttca attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa
    39301 attgatcata tggtcttggt atcggttcaa aggctccaaa taaataaccg tacctgtttc
    39361 ttcggctact ttatctaaat aacgaagtga ttcgctgact gctttatagt ctccttcttt
    39421 gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac attccccaag ctgctgggat
    39481 aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac
    39541 accattaaga cgccgttctt cgataaagtc accgatccac ccatcgtagc cgttacaagc
    39601 tgtcactact gggatccctg tttctttgat tgcctgtttg actgtgtcga tattttctat
    39661 taacaactta ccgtcgattt cgtattcttc aaatccacag tctttgataa atctaaattt
    39721 ttctaaaatg tctcttggga aaaatgcttg atcttgcgta ccgaatttca ttgttctcct
    39781 cctcggactt gaacgcccat tttgatactt tcttctgggt gctggtctac ataacgcatg
    39841 taagcttctg cgctttctgc aaatggcaca actggatcaa tgatttcttc acaatccaag
    39901 taaccgttca tcaataattc ccaacatgtg tcctcgattc gtttacggtt ccatcttggg
    39961 taatctgggt ttggttcact agatgctcga gcaaagacga tttttgcatt attgaaatgt
    40021 gcttcgcgac caagattaaa cccagctgga aaaggtttag caaaggcaac ataagaaatg
    40081 atcccgccat agccgatccc tattaaagaa gcttgtaatg cggcagcact gccactcgtt
    40141 tcaataactg aatctactcc acgtttatct gtcaattcct ttagacgaaa accgacatct
    40201 gctttcatag gatctagcgt gtaatcggca ccgtgttttt ctgctaattc tctacgctgc
    40261 gctatgggat cgatcccaac gatggtagta gcacctgctt ttttagctaa ttgaatcgca
    40321 atttgcccga tagcgcctaa accgaagatt gctacagcat cacctgctcg aacatgtgct
    40381 tcccggatac cacctaaagc gaattgagca ggatcgtaac atactgcatt tttccattga
    40441 tccaaccgat caagtttacg tagtttataa ttatcaacac cgtttgtaat cagcgtttca
    40501 cgaatgggtc cataagtgca gactaactct cctttttgat actctgtcac caaactgccc
    40561 gtttcttcga tcgtaccgac aatcatattt cctaattgaa attcgccaaa taccacaccg
    40621 cgttggcttc cttcttctct tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg
    40681 ataaatggac ttaatcctct aaagtcgaca atttctgttc ctggttttgg agtagcgaaa
    40741 attactcgga tacgtacttc gttcttttcg atctcacggt cttcgtaatc tttgaatccg
    40801 gctattcttg gttctaatgc aactaatttt ttcatctttt ctttcctctt tcttttcttt
    40861 ttatcctttt acgccgcctt cgactaaatc acctttaata aatcgttcag ataacgcata
    40921 cataatgatg actggtaaag cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta
    40981 gtctggtgta ctgaataatg tgttcaaacc gattggtaaa gtaaattttt cggaggtcga
    41041 caggaaaatc gaagcaaata aataatcatt ccacgaaacc ataaagcaat aaacgaagac
    41101 agaaatgatt ccagaaatag ccaatggtat aatgatccga ataatgatct ggatccggct
    41161 aagaccatcg atcattgctg cttcttcaat atcagtcgga atcgtgtcaa aataactttt
    41221 tagcatgaat atcgcagtag gaagtgtttg aacgaccatc gtgataatca gtgctgtatt
    41281 ggtatcatac aaacctagac cggaaatgat tttaaataat ggaacaacga tcaaaatacc
    41341 ggaaaacata taaacggtgt aaaagctagc attgattttc gagcgagctt tgaagcgtag
    41401 ttttgacaaa gcgtatgccc cgctgatccc aatcactact gcagttacgg aggctatcaa
    41461 ggagatcact aaactatttt tgaaatacat gatgaatgga aaaatctcag cattgaaaat
    41521 atcctgataa tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc
    41581 ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa aatggaaaca aattgacagc
    41641 taatagcaac aaaatcgtcg cgtaaaataa gattttcctg ctctttttat ttttcatttc
    41701 aagtccacct tctttcgtga gagcaagacg accgctgcaa taatgatgaa caatagaatc
    41761 gatatagctg ctgcttttcc aagatcattg aacgcaaagg ctgtctgata caaataaacg
    41821 cctaagatat tgactttatt cgtcagtaga tagacatctg taaacatata gaacatccag
    41881 atagatcgta aaacaatcac agttgctagt actggtttga ttgccggcag tgtcacgatc
    41941 ttgaacttct gccaagcatt tgcaccgtcc atttcagctg cttcataaag cgttttgtcg
    42001 actgtttgca aaatagccaa aaatgaaata aacgcatacg gatagtatct ccagatagca
    42061 aacaaaacga ccataaagaa gctagcaact ggctggtcga accacagcgg tgcttcgttg
    42121 aaaagctgca acctatctac taacagatca ttgacgattc cataactatt attgaacata
    42181 tatttccaag caaaaatcaa agaaatagat ggcgtaacat aacttaataa aatcagtgaa
    42241 cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa
    42301 gctgtactgc ctgctacaac taagaatgtg taagcaactg ttaaacctaa agaacgataa
    42361 aattcactat cacttaaaat ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag
    42421 ttaggattca gcggcatatc gaaaaaactg atctgaatat tggaaagcat tggaatcagc
    42481 actaaaacag ctaataaaag catgcttggt gctaaaagtc ccatggctaa ttttaagtca
    42541 gaatgtttcc ttggacgatt cattgaaacc tccccctgat aatctgccaa tagctatcta
    42601 cttttttcga acaccattgg cagaacttct tgttatttcg tatttttttg tgcgtcagct
    42661 aaagcttgat cgacatcttc gtttccgaca gttacttggt tcactgcttt tccgatcaca
    42721 cctgagcttg tgatatctcc cattttcgtg aaattctttt ccccgactag tccaaagact
    42781 tgtacttcat caaaagcact agcaatctca gatggcagct cgccaaaaga cttgatgact
    42841 tcattttctt tgtaaccatc cagttcgact acttgtttat tcactggttg agcaccacca
    42901 ggagacatca agacccactt ttccatgttt ttcggttgag ataagaaagc tgtgaatgct
    42961 tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg
    43021 ccatagacag cttgatcttt ttgtgttggg atagcaaaac cgatattttc tgagttcccc
    43081 gcttcataaa cagaaggcaa aatgtaagtt gaatagattg ccattggtgc tgttccattc
    43141 atgaaagcat ctttgatttc tgtcacatca ttagagcctg gcatcgtgta ttgggataag
    43201 ttttgataaa acgacaatgc ttctctcatc ttttccgtat cgatcgttac ctcgccttca
    43261 tcatccaaaa catttgctcc atttgacaaa gcaaattgag agaacgcttg ctcactcatt
    43321 gttccttcag cagtaggcat agcaattcca tattgtttat tctctttatc tgtgaaatgt
    43381 tgagcaattt ttaaaatatc atcccaattt tctggttctg aaaaaccagc ttctgataat
    43441 ttttctttgt cataccaaat accttgaacc cagccgctaa ttggtgcagc aatatagctt
    43501 tttccatctt ctgaacgaac aaggtttttg gctccgtcat aataattatc ttctccagct
    43561 tcctcgataa cgttttggac tgccgtctga tcaatcagtt catctttatc catcacttta
    43621 gcaaaatcct gactgacttc cattacttct ggtaatttac cggaacgagc taacgtaacg
    43681 atttttgtat tgaacgctga ttcttctacc ggcacttgct tgattttgat gtctggattt
    43741 tctttttcaa aatcagcaac cagttcattg atcacactta atcgttcctg ttcgacagaa
    43801 gagtgcatga attgaatcgt tgtctgttgt gattcgttag agctttgact ttcaccacaa
    43861 gcagtaagaa gaaatccagt agctagcaga caggtagtaa caataatttt tttcattttt
    43921 ttccacctct tattttatta ttttcagcca catatattca tacggctgga atatcaactt
    43981 tcgttcttga ctgcaatgga taatttgtcc agaaaacagg tctaggtaat ctccttttgg
    44041 taaaagataa cctgctgatt gattggaaaa attatggata actaagactt tctcttcctt
    44101 cgcttcgcga ataaaactga taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt
    44161 cggatggaag gctttttctt tttttcgaat agatagcatc tgggtcaatt caccatatac
    44221 tgctgttctt aatggatctg tctgcaactc tttctcaata tctgctaaca aaaatttttg
    44281 ccgattgatt tttcgattct ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc
    44341 cagcacactt tgaatataaa ttgccggcac gcctggtaac gttaccaaaa atgaatgagc
    44401 agctaaaaag cgtttgcttc tccattccgt cgtatctgtt ggagaactta acgcatctag
    44461 atacgtagcg tttatctcat atggactggt cgttccatct gggtttttct tgtagtttac
    44521 tcgggcaccc tctttcttca gctgttccac taattcaagg atcgtttttt ccggcagaat
    44581 acctcgtacc ggattcaatc cgataccgtc atgggaggct aaaaaattaa aaaaagttgt
    44641 ttttttcgat gcgaaatcga tggtttttaa ccattctttc aacttacttg tattacccga
    44701 acgaatcgct tgaagggtca aaggtggtag cgaaaactga tagaccatct gtgcttcatt
    44761 ctcgccattc ccaaaataac tgatattatc tttgtgtggc acatttgtct cagtgatcaa
    44821 taccgttcct ggagctgctt catcagcgat tgcacggaat aattggatga ttttatgcgt
    44881 ttctggcagg tgtatacaag aagttcctag ttctttccat aaaaatccca cagcgtccaa
    44941 gcgaataaat gaagcgcctt tttctaagta aaacaaaaga atatcgatca ttttcaataa
    45001 aacttctgga tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg
    45061 tttttctttc ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc ttggtctagt
    45121 cacgctgctt gtatctgtat ccagtggcaa atcaataaaa aaatctttgt acgtatcatt
    45181 caagtttaaa tattcctgaa accaagtact ttcggcagac atatgattgc agacaaagtc
    45241 gaacattaac cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc ccaaacggtc
    45301 gttgattgcc tgataattta caacggagaa accatcatca gatgtatatg ggaaaaatgg
    45361 caagaaatgt acaactctaa atgtatctgc caaatacttt tgataaaatt tcttaaatgt
    45421 acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca taagtaatca agaaaatatc
    45481 ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc tgctgataaa cactgacttt
    45541 ttgttcaatc atagaggcgg ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa
    45601 aagttttgta tacatcggat tcccctttct tttttgggag cgttcccaac gatttgatca
    45661 tattatggaa aacgcttact gtcaatagca tttttttaaa taattttgat atacacaaaa
    45721 atacaaataa gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa tatggtctct
    45781 catgatcatg caagctattc ctaaattttc aaaatcatag acaaaaaaga atgtctattg
    45841 tactatggaa ttattctaaa acgctcccaa aaaggaggtg catataaaat tgaaacctac
    45901 tatttatgac attgcgaaaa aagctaatgt ctcaaaatca actgtctcca gagtattaaa
    45961 taaccagcca aatatctctg ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt
    46021 gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga aatggttttg acgcgattct
    46081 agcattatct catcgtactt ctcatacgac ctatggcaat cctttttttt cagatgttat
    46141 tcaatccatt tctgccgttt ctgaacaaaa taacttcgac ttgattctac aaacggcaaa
    46201 aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg
    46261 aattattatt ttaagctcta cgatggatga aacacttctt tttgaattgg atcaatataa
    46321 tattcctatt gtggtaatcg gaaaagtcgg aaacgactac acgaacatct actcagtgga
    46381 cacggataat ttccaggata gttacgattt ggtggaaaca ctgatcaaga atggacaccg
    46441 tcatatcggc tgtctccatg ctccgacaga agtttatgta tccaatgatc gtctcaatgg
    46501 ttaccgtcaa tgcttatttg atcaccacct agatatccgt aatgaatgga ttatagacgg
    46561 cggctttcgc ttagaagata gcatggcagc aatgaatgat ttaatgaagc tacgagaaag
    46621 accaactgcg attttttcta ccgatgcatt aaaatcattg agtatttaca aagtggcaga
    46681 acaatctgga aagcagatac ctgatgatct ttcgatcgca ggattcagtg atcccttatt
    46741 gtcctcccta ttttcgccac agttgactcg gatagacatt ccgacaaatc agttaggaat
    46801 caaagccaca gaacttttat tccaactaat ccaccaccaa taccctgaag aaaaaagaat
    46861 cattctttca accgagatta ctctcacaga ttcgatccaa caacaagaaa tgtagaacag
    46921 aattataaaa gaacagaaac actgaggata agtcagttac tctgttcttt tttcaatcac
    46981 tcatccctcg ttaccccctc ccggtatttt agcgaatttt tcttattttc catctgtttt
    47041 agatagctgt ggataactat aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa
    47101 actattttta accacgttgt gtataagttt ttatagttcg tttttttaaa cacatttacc
    47161 cttatttcaa tccatgttat ttggggatac ttatctctaa aacataaata gaataacaga
    47221 catgtctact ggttttgttt tattcgagga attattcaat aatagctagc aaaatattaa
    47281 aaataaccgc tctatcttcc catcaattta ttttgcattc aaaaaatgat ctatctttac
    47341 ttttgttgat tgacttctct tggaagatga tcgttccgat accttaattg gatacgaacc
    47401 gatgttgtta agatcctttc ccacttttct tctagttaga caaattattt tagacaaacc
    47461 aaaaaagagg ttgtgacaat aatttgtcac aacctctttt ttaaaatatc tattttgttt
    47521 gattgttcat tgcgtcttct gcagccattt gcgcttcaat atctgaaata ctgtagacat
    47581 tttcgcttgc tacaccgact tcttttggtt ccatgtcacg ataacgttgc ataccagtac
    47641 cagctgggat gatcttacct ataataacat tttctttcaa accaagaaga ggatctttct
    47701 taccgcggat cgcagcatcc gtcaagacac gagttgtttc ttggaatgat gcagcagata
    47761 agaaactatt tgtttctaat gatgcttttg tgatacctag taagacaggt cgagaagttg
    47821 caggaacgcc tcctgagact agcgtattgt agttttgatc tttaaagtct gcgatgtcta
    47881 ataatgtacc tggtaagata tctgtatcac ctggatccat tacacgaact ttacgcagca
    47941 tttgacgaac cattacttcg atatgcttgt cgccgatttc taccccttgc atacggtaaa
    48001 cacgttgtac ttcacgaagc aagtagtttt caacagataa tacatcgcga acttgcaaca
    48061 attgttttgg atcgattgat ccttctgtca atggtgcacc acggtggatc atatcgcctt
    48121 ctgctacttt catacgtgct gtataaggaa cagtatacgt acgtgtatct gtttttcctt
    48181 tgattgttac ttctttggta cgtgttgcag gatcttctga gatatcgatg acatcccctg
    48241 ttacttctgt gatcactgct tgccctttcg gattacgtgc ttcaaagatt tcttggacac
    48301 gaggaagacc ttgagtgata tcgtctccgg caaccccacc tgtatggaat gtacgcatcg
    48361 tcaactgagt accaggttca ccgattgatt gagcggcgat cgtaccaact gcttctccga
    48421 cttcaacgtc agagccagtt gcaaggttgc gtccgtaaca atgtttgcag acaccatgtt
    48481 ttgtattaca tgtgaagaca gaacggattg tcactgtttc gacacctgca tcaacgattt
    48541 gttttgcgat gtcttcggaa atcaattggt ttcctgcaat gatcaattca tttgtttcag
    48601 gatgacgtac ttctttacgg gtgtaacgtc ccactaaacg atcttctaat gattcgatga
    48661 tttcatttcc ctcgcggatg gcttggattt caagacctcg gtcagtaccg caatcttctt
    48721 cacggatgat tacatcttgg gccacgtcaa ccagacgacg agtcaagtaa cctgaatcgg
    48781 cagtcttcaa ggctgtatcg gtcatccctt tacgagcccc gtgagttgag ataaacattt
    48841 ccaagacgga tagtccttca cggaagttcg aaatgattgg caattccatg attcgaccat
    48901 taggagcggc catcaaacca cgcattccgg caagctgtgt aaagttggaa atgttaccac
    48961 gagctccaga atcagacatc atgaagattg gatttttcgc ttccaaacct tccatcagtt
    49021 tttgttggat gctgtctttt gcagcattcc aaacagcaat gacacgttca taacgttcgt
    49081 catctgtgat cagaccacga cggaattgtt ttgtaatagt ttcaacttgt ttatgggcat
    49141 tttcaatgat ttcttgtttt tcattcagta cactgatgtc agcgatccct actgtgatac
    49201 cggcatgtgt cgaatgtttg taccctaagt ctttcatacg gtcaagcatc ttagaagttt
    49261 ctgttacttt gaatcgtttg aagacttctg cgatgatgtt tcctaagttt ttcttcttga
    49321 acggtaatac taattcttgc tctttgatat gtgcaggaat atcagtacca gcttcaacaa
    49381 agtatttgtc cggtgtttgg acggtcaagt tgaagtctgt tggttcattc agataaggga
    49441 attcaggagg catgatctcg ttgaagatga tcttaccaac tgttgtgatc atcgtacgtt
    49501 ctttttgcca ttcagtaaat ggtttttcac ctaatgagtt agggttcaca ccgatacgtg
    49561 aatgtaaatg aacatatccg ttgcgccatg cgataacggc ttcgttttga tctctgaaga
    49621 ccattccttc gccttcgcgt ccttcttctt ccattgtcaa gtagtagtta cctagaacca
    49681 tatcttgaga aggagtaaca actggtttac catctttagg gttcaagatg ttttgagcag
    49741 ctaacatcaa catacgtgct tctgcttgtg cttcttcatt caatggaacg tggacagcca
    49801 tttggtctcc atcgaaatcg gcattataag cttcacatac aagtggatgc aaacggattg
    49861 cgcgtccttg tactaaaact ggttcgaatg cttggatacc aagtcggtga agcgtaggtg
    49921 cccggttcag caatactggg tgttccttga ttacgtcttc taaaacatcc catacttcgt
    49981 cttcttgacg ttcgatttta cgtttcgcat ttttgatgtt tgaagctagt tcgcgttgaa
    50041 caagctcacg catcacgaat ggtttgaata attcaatggc catttctttt ggaagtccac
    50101 attggtacat cttcaagaaa ggtccaacaa cgataacgga acgacctgag tagtcgacac
    50161 gtttacctag caagttttga cggaaacgac cttgtttccc tttcaacata tgagaaagag
    50221 atttcaatgg gcggttacct ggtccagtaa ctggacgtcc gcgacgaccg ttatcgatca
    50281 atgcatctac tgcttcttgc agcatacgtt tttcattttg aacgatgatt ccaggtgcat
    50341 tcaagtctaa caaacgtttc aaacggttat tacggttgat tacacgacgg taaagatcat
    50401 tcaagtcact tgttgcaaaa cgtccacctt ctaattgaac cattgggcgt aaatctggtg
    50461 gaataacagg gataacgtcc ataaccatcc agcttggttg atttcctgaa gcgcggaatg
    50521 cttctaaaat atccaaacgg cggattgcac gtgtacgttt ttgtccttgt gcagatttca
    50581 attcttcttt caattcggct gcttctgcat ctagatcaac attatcaagt aattgtttaa
    50641 tcgcttctgc acccatagca gcttggaatt cttgtccata ttgttcacgt ttttcgcgat
    50701 attcgcgttc tgtcaacaat tgttttttct ctaaattcgt atttcctggt tcgatgacta
    50761 catatgaagc aaaataaatg atttcttcca aagcgcgtgg gctcatgtct aatacaagac
    50821 ccatacgtga aggaatccct ttaaagtacc agatatgtga aacaggagct gctaattcaa
    50881 tatgtcccat gcgttcacgg cgaactttcg aacgagtaac ttccactccg catcgatcac
    50941 aaacgattcc tttataacgg atacgtttgt attttccgca ggcacattcc cagtcttttg
    51001 taggaccaaa aatccgctca cagaacaacc cttcacgttc aggttttaag gtacgatagt
    51061 taatcgtctc aggttttttt acttcaccgt aagaccagct tctgattttt tcgggagaag
    51121 ccaaacctat ttgcatactt tcgaatttat ttacatcgat caaaaggggt tccctccatt
    51181 tcatttaatg gcaaaccgca agacggctga tcttcgaccg ccttgcagct catagaccgg
    51241 attagtctaa attatcttct gcggtttcaa agttttggat gacatcgtct ttttcatctt
    51301 ccacttgttc agcagctttc ttttctaatt ctttggcagt ttgctgttcg gcaaattttg
    51361 tcaaagcatc aacagtgatc aaatcgtcgt cttcatcgtc catgtcgcga agttcgatct
    51421 ctgtttcttc gatatccaag acacgcatgt ccagacctag tgattgcaat tctttcacca
    51481 atacgcggaa tgattccggt acacctggtt ttggaattgg ttcacccttg acgattgctt
    51541 cgtatgtttt cacacgtccg actacgtcat ctgatttgta tgtcaagatt tcttgtaaag
    51601 tataagccgc accgtaagct tccagtgccc atacttccat ttcaccaaaa cgttgtccac
    51661 caaattgagc tttacctccc aatggttgtt gtgtaaccaa tgagtaaggt ccgattgaac
    51721 gtgcatgcaa tttgtcatcg accatgtgag caagtttgat catgtacatc acaccgacag
    51781 acacacgtcc atcaaatggt tcacctgtac gtccgtcgta cagaacagtt ttcgcatcgc
    51841 tggccatacc ggcttcacga actgtttccc atacgtcttc atcacttgca ccatcaaata
    51901 ctggcgttgc tacgtggata cctaactgac gtgcagccat acccaagtgt aattccaata
    51961 cttgcccgat gttcatacgt gaaggcaccc ctaaaggatt cagcatgata tcgattggtg
    52021 ttccatcagg taggaatggc atatcttctt ccggcataat acgggataca acccctttat
    52081 ttccgtgacg accggccatt ttatcccctt catggatttt acgtttttga acgatataaa
    52141 cacgaactaa catgtttacg cctggtgaca attcatctcc agcttcacga gtaaagattt
    52201 tcacatcgtg aacgataccg ccgccgccat gtggtacacg taatgatgta tcgcggactt
    52261 cacgagcttt ttcgccaaag atcgcatgca atagacgttc ttctgcagat aattctgtta
    52321 ccccttttgg cgtgactttc cctacaagca aatcgccatc ttggacttct gcaccaatac
    52381 ggatgatacc catttcgtct aaatctttta atgcgtcttc cccgacatta gggatttcgc
    52441 gtgtgatttc ttcaggtcct aattttgtat cgcgggcttc tgattcatat tcttcaatat
    52501 ggatagaagt gtatacgtca tcttttacca aacgacggct catgatgatc gcatcttcat
    52561 agttgtaccc ttcccaagtc atgaaaccaa ctaaaacgtt ttggcctaga gccatttcgc
    52621 cttgttccat agatggtccg tctgccagag tatccccttt ttcgactttc tcacccaaat
    52681 gaacgattgg acgttggttg tagcttgttc cggagtttga acgacggaat tttgttacat
    52741 cgtatttatc taatgcaccg ttgtcgcggc gtacgcggat ttcagaagca tcaacgtatt
    52801 caacgacacc gtcatgttta cataacaaag cagctcctga gtcatgcgct gatttgtatt
    52861 ccataccagt accaacccat ggtgcttgag gattgatcaa cggcactgct tgacgctgca
    52921 tgttggcacc catcaaggca cggttggagt catcgttttc caagaaagga atacatgctg
    52981 tcgcaacggc aactacttgt ttaggtgata cgtccatgta gtctacttta tcaatagata
    53041 cttctaagtt ttcactttga gcacgagcca taacaacgtc ttgagcaaat gttccatctt
    53101 cattcaatgg gctgtttgct tgggctacga tgtaatggtc ttcgatatca gctgtcaagt
    53161 agtcgatttg gtcagtaaca cgacctgttt cacggtctac acgacgataa ggtgtttcga
    53221 taaatccgaa tttatttact ttcgcataac tggacaaact attgatcaac ccgatgtttg
    53281 ggccttcagg agtttcgatc ggacacatac ggccatagtg ggagtagtga acgtcacgaa
    53341 cttcataacc ggcgcggtca cgagtcaatc cgccaggtcc taaggctgaa agacgacgtt
    53401 tgtgtgtcaa ttcgccaaga gggtttgttt gatccatgaa ctgtgacaac tgagaagaac
    53461 caaagaattc tttgatactt gccacaactg gacggatatt gatcaattgt tgaggtgtca
    53521 atgtttcagt atcttggatc gacattcttt cacgaaccac acgttccata cgtgctagcc
    53581 cgatacggaa ttggttttgt aaaagttcac cgactgaacg gatacgacga tttcctaagt
    53641 ggtcgatatc atcgacattg ccgatacctt ccatcaagtt caagaaatag ctcattgaag
    53701 caatgatgtc ggctggacga accgttttta cagctgcttc tggatagccg tttccgatca
    53761 cgtttacttc acgttcaggg tctcttggtg aaaatacttt gatgacttgg acagtcattg
    53821 gatctgtgac cacaccgtct tctgatggat agtaagtcac tgaatttagg ccattatcaa
    53881 tgaaaggagc tagtgtttcc atgacttggt gagtcagaac tgttcctttt tcaacgatga
    53941 tctcgcctgt ttctggatca actaatgttt cagctaatgt cagattcaat agacgtgttt
    54001 tcaaatctaa ttttttgttt actttgtaac gaccaacgtt tgctaagtcg taacgttttg
    54061 gatcgaagaa acgagcattc agcaagttac gtgagctgtc tgctgttttt ggttcgcctg
    54121 gacgcaaacg ttcgtaaacg tctttcaaac cttcttctgt tcttgagtcg cttgcgtttt
    54181 tatgcaagtc tttttcgact gtattgcgta atgtttcgct gtcgccgaag atttcaaaga
    54241 tcgtatcatc ggaaccaaat cctaacgcac gaactaatac ggttaaagga atcttacgtg
    54301 tacggtcgat acgtacataa gagatgtctt ttgcatcagt ttccatttct agccaagctc
    54361 cacggttagg gataacagta gaaccgaatc cttcttttcc gtttttgtcg acttttccat
    54421 ggaaatagac acctggtgaa cggactaact gagaaacgat tactcgctct gccccgttga
    54481 tgatgaatgt tccttgttct gtcattaatg ggaaatcacc gaagaatact tcttgtgctt
    54541 tgatttcgcc agtctcgcgg tttgtcaaac gcaatgttac atgtaatggt gcagagtagt
    54601 ttgcatcatg cgcacgagct tctgctactg tatactttgg ttctttcaat tcataatcta
    54661 caaattccaa agatagattg ccattgaagt catcgattgg caaaatgtct tcaaacattt
    54721 ctctaagtcc ctcatctaag aaccattggt aagagtctgt ttgaatttcg atcaaatttg
    54781 gtaattccaa cacttcactg attcttgcga aacttctacg ttcgcgatgt tttccgtatt
    54841 ttactacgtg tccagccaag ctcttcaccc ctctaaaatg tcattcaaaa agactacccg
    54901 atcaattgtt acaataatat ttcaaatatt atttttaggt aacaacagac attttttgcg
    54961 gcttttaggc aaaaaaaaac caaaaataga acattctctt gaaaccttac ttcgtgagct
    55021 tctacttttg tttccttata ttaaagccga aacggacaat atttcgtttc tgccttttct
    55081 tcatcagata gtttttgcaa cttcatatac tacacttatt ttctataaaa gtcaacactt
    55141 cctggcgttt cttctgcttt ttcgcaacat tttcttaatg ttttcaattt tatgttttca
    55201 attatctgac aattcttaat tgacagacct ttttatctcg gttatacttt agaaaattca
    55261 ttttgtggag gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat
    55321 aacttttact ttagctactt tagataggtt tgtattcatc atgatggata caaacgttaa
    55381 acgacagttt gtatctatga tggctaaaga actttgtatt gcagcaaaaa gccacgtaga
    55441 caaaatgcta agtggctgag cagtgaaaat acaaagtaaa agtatcaagc attctacttt
    55501 ttcaatttat tttccgactt ttcaatccat ttggacattt tgtctgaatg gattttttgt
    55561 ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg
    55621 ccctgctact agctttagga ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg
    55681 gacaaaaaac aattggcgta ttgcaacttg tacaacataa ctcgttagat gctgcctata
    55741 aaggattcaa agaaggatta gcagaagaag ggtataaaga aggcgataat ctaacaatcg
    55801 attatcaaaa tgcccaaaac aatcaagaca acctgaaaag tatgagtgag aaactcgtca
    55861 aagaagatcc tgatttgcta cttggaatcg ccactccagc agcacaatct ttattaaatg
    55921 aaacaaccac tatcccgatg actgtcacag cggtcacaga cttggaacaa gcccatttag
    55981 tcacatccaa tgaaaaacct ggcggcaatg tgactggcac gacagatatg gtgccaatcg
    56041 aaaaacaaat cggcttactg ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt
    56101 ataacaacgg tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa
    56161 aagcgggcgt caaagtcaaa gtactgacag caaattccac aaatgatgtc caacaagtga
    56221 cgaaaagtct agctaaagat gtagatggaa tctatattcc tactgataac acgttcgcaa
    56281 gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg
    56341 cagttgatca agtcaaagac ggcggtctag caacagtagg aatcgactac gaaagcttgg
    56401 gcaaacaaac agggatcatg gcagcaaaaa tcttagacgg caagaatcca agtgaacttc
    56461 ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa agacatggcc aaagcattag
    56521 gcatcaatcc agacagcatc aaagaagctg aataaaacac aggaggattc tcatgacaaa
    56581 tttgctctct cttttcttat catctgcatc gcaaggggtc ttatgggccc tacttgcgat
    56641 tggtgtatac gtcactttcc gtatcttgga cattgccgac ttgactgctg aaggaagctt
    56701 tcctttaggt gcagggatcg cggcagtaag tattacaaat ggctatcatc cattgacagc
    56761 ttgtcttcta ggctttttag ggggtgcggc agcagggctt gtttccggac tgcttcacac
    56821 aaaattgaaa attcccgccc ttcttgcagg gatcatcacc atgactggtt tatattctgt
    56881 cacatcccgt gtgatgggtg cgccgaacat ctctttattg ggtcagccaa cgatctttca
    56941 ttgggtcgag tcattcggca taacaaaaga caacgctgta ctgatcgtcg gtctgctttt
    57001 tgctttgatc gttgtatgtt tacttgtttt gttcttacgt acagaaacag gacttgctat
    57061 tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac gggatcaaca cagataatat
    57121 gaagatcatc ggttatatga tttctaatgg atgcattgct ctttctggct ccttacttgc
    57181 gcaaaacaac ggctttgctg atttaaattc tggtattgga acgatcgtaa ttggacttgc
    57241 ttctattatc atcgcagaag ttttgttacg taaccagcca ctatttctcc gtctcttgac
    57301 tgtcgtttta ggtgcggtca tttatcgttt tattttagcg ttagtcttcg aactgaatgt
    57361 tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc
    57421 gcagatccag caaaaattcc atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat
    57481 gacgtcttaa cattggaaaa tctccatcat acctttgaaa aaggaacgat caacgaaaat
    57541 catgtcctga aaggcattaa tctttcctta gatcccggcg acttcgtgac gatcatcggt
    57601 ggaaatgggg ctggtaaatc tactttgctg aacagcatcg ccggtacatt ttctgtcgat
    57661 cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg
    57721 aaatccatca gtcgcgtatt ccaagatcct aaactaggga cagccgttcg tttaacggta
    57781 gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc
    57841 aaaccacaag accgaacatt tttcaaagac cagctggttc ggctaaattt ggggttggaa
    57901 aatcgtttat cctcagaaat cggtttgctg tctggaggac aacgtcaagc tattacgtta
    57961 ttgatggcca cattacagcg tccagaactg atcttattag atgaacacac tgcagcgctt
    58021 gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa agctgatcac tgagcagaat
    58081 ctcactgctt ttatggtcac tcatgacatg gaagacgcga tccgctatgg gagtcgactg
    58141 atcatgctgc atcaaggaag gatcgtcgta gatatccagc aagaggaaaa acagcagctc
    58201 acagtgcccc aattattagc gttgttcaaa caaaacagcg gcacggcatt aaaagacgat
    58261 caagtacttt tatcttcata atcagaaaga ggatgggaca aaaaatgaac gcccaccctc
    58321 ttcttttttc tctttccttc tttcgttttt gctttatgta tcaaccgttg tttcttcgaa
    58381 aactattttg ttctctcctt cttcctgata gaatggatgg gaaaggagct tgatgccatg
    58441 acaacgaaag acaaggtact tcaattattg aaaaagacac cagattttct ttccggagaa
    58501 aagttggcac agcaactgga agtttcccgg acaagtattt ggaaagccat caaagaattg
    58561 gaaaaggcag gctatcgttt tgagcacggg cctacagggt atcgttacct gccctctgac
    58621 gtactagatg caaaagaaat ccagcaagag ctacggtcgt ttattcctga tttgaaagta
    58681 caaacgatgt tgacttctga atctacgatg aaagatgcca aactggcagc agctcaagga
    58741 gattctgttt cgaagttatt tgttgctgat acacaagaac aagctcgtgg acgttttgga
    58801 cggccttttt tcgctgaacc tggacgagga atctacatga gtctcttgct ccatccagac
    58861 aaacggtttg atgaattgcc tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc
    58921 attgaatctg taaccggtcg ttctgcttcg atcaaatggg tcaatgatat ctacatggat
    58981 ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg atatggaaag cggcaggatc
    59041 agtcatatca tcattgggat cgggcttaat ttttctgttc ctcaaaatca ttttccagct
    59101 gatctccaag aaaaagccat ctctgtcttt cccgacggac aagctagtgt cacaaggaat
    59161 cagctaatca gcaaaatctg gcaagaattg ttcacttatc tttctcaccc agaaaaagcg
    59221 ttggacttct ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa
    59281 ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa
    59341 acaaatgaac agaagatgag tttgtcttct ggcgaaatca gtctcacatc tatccgtacg
    59401 caataattat cagatttcac aaaaggacac ctgatctcat acagtgtcct tttttctgaa
    59461 ttctcttcaa aagactctcc ttattctatc agctgacagt ttctctatcc tctagaaaaa
    59521 ggtattctaa aaatatctat aaagcatggt acactttctt taaatgaatc aatgcaaaaa
    59581 acaagagagt catcagttta cacgatcaaa agagatagca ggccgcttcc tttttcaatc
    59641 ttatagatag accatgggta acgataaaaa caaaaaagaa cttgcgactt attttgccgt
    59701 aagttctttt ttagctagtg ccttaatctc ttaatcccaa caaacgtctc gcatagatca
    59761 cctgaccaga gtgcatagca gcatcatcaa taatagaaac gagacgcatt cctcgtgtga
    59821 ctggtggtgt ccagttctcg tcaattactt catctagctc ggaaggttcc agccttctta
    59881 agtaagaaat agcaaaatca gcagctcctc gcaagtatcc tagcagatct tctatttgat
    59941 tagcttggat ctgctttgct tgttccagac tatgcttcca atcttgttcg ttatctgcta
    60001 tcgtcatcgg aaaccggctt tcccagtctt gactatgcca aattggagaa gttcctgcta
    60061 aatcagcaat ctggaaatct aattctcgag cggtatgcca agccagccaa gtcattgatt
    60121 tgatctgcgg ggctctatct tccactggaa aacgattggc ctcctcaata gagacgcctt
    60181 caaacaaacg ttcaaaccga tcgtatgccc gatccacact ctcgcatagc atatctaaat
    60241 aatccattta ttttcctcct ttttatttat cctacttact ttttcctaat tctaggatac
    60301 ctacttatat gttactacac acccttattt tttacacttt aacagtcacc atcatcaata
    60361 gtttggagat tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga agcaacaata
    60421 tgaacgtttc attaatattc tttaaataaa caatgatttt tcaaacgaac agtttaagac
    60481 taggagaaaa agagtataat aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa
    60541 aaagggcaag tttactagtc ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg
    60601 ccgtttcagc ggaaacatac attcctaaag accctgggaa tatttataat gatctaactg
    60661 gtaactatgg cttgttagga attgctagtc aattccacgt atttgctaaa aataagacaa
    60721 ctgtgaatgc acatacagac ggaaatatag caacacatga acttgatgca aaaaataatt
    60781 ttggtacaga gcttcatgaa ggtttattga accaagaaat aaattatata caaaaaactg
    60841 attctttcgc taattcttca ggcataccta caggtagcac aagaatgaat aaatttgtcg
    60901 ttggcagtaa cgtggatgta gcacttcaag atggtcatcc tacagtcaat ggatccaggc
    60961 ttgatcactt aactccagat gatttttata aagatcgtgc tgggcatgaa tatattgatt
    61021 tcgagcagga atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg atcactccag
    61081 ctaaaacata tactgctgca gattttccag acatgaacaa tcggacaatc gatttaactg
    61141 atttatcaga taccgggttt ttattggtaa atatcgatgc agaagtgtta acgatgaata
    61201 cgccacttca aatcattaat ccaaatgatc aagtagttgt atttaatgtc atcaacagtt
    61261 cttctgcatt gaacgtacag tcaccaatca aatacaatga tcgttcaaat catgaaacag
    61321 aagatttttc ggatgcgaat atctcttgga attttggaaa tgagatgact gatttgacaa
    61381 tcagtgctcc ttttcaagga acaatccttg cgcctaatgc tactatccgc attactcaaa
    61441 accaagacgg aacgattata ggaacaaatg tgattttaga tgcagctacc aatcgttggg
    61501 atcctaacga aatatttatc agcgacaatg gtacagatac gacagacaca agcgacagta
    61561 cagatacatc taccacaagt gatagctcag attcatcaac ttcaagcgac agtacagata
    61621 ctacctctac cacaagtgat agcacggata catcagcttc aagcgacagt acagatacta
    61681 cctctaccac aagtgatagc acggacacat cagcttcaag tgacagtaca gatactacct
    61741 ctaccacaag tgacagc
//
LOCUS       DANJLF010000009        60875 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000009 DANJLF010000000
VERSION     DANJLF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="PWT25_000655"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="PWT25_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL1020760.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="PWT25_000656"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="PWT25_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL1020761.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="PWT25_000657"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="PWT25_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL1020762.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="PWT25_000658"
     CDS             4735..5628
                     /locus_tag="PWT25_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020763.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="PWT25_000659"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="PWT25_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL1020764.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="PWT25_000660"
     CDS             7565..8422
                     /locus_tag="PWT25_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL1020765.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="PWT25_000661"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="PWT25_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL1020766.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="PWT25_000662"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="PWT25_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL1020767.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="PWT25_000663"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="PWT25_000663"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL1020768.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="PWT25_000664"
     CDS             11178..11936
                     /locus_tag="PWT25_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL1020769.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="PWT25_000665"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="PWT25_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL1020770.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="PWT25_000666"
     CDS             12864..13895
                     /locus_tag="PWT25_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL1020771.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="PWT25_000667"
     CDS             13892..14659
                     /locus_tag="PWT25_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL1020772.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="PWT25_000668"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="PWT25_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL1020773.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="PWT25_000669"
     CDS             16624..16944
                     /locus_tag="PWT25_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL1020774.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="PWT25_000670"
     CDS             16944..17294
                     /locus_tag="PWT25_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL1020775.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="PWT25_000671"
     CDS             17461..18399
                     /locus_tag="PWT25_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL1020776.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="PWT25_000672"
     CDS             18414..19244
                     /locus_tag="PWT25_000672"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL1020777.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="PWT25_000673"
     CDS             19288..20313
                     /locus_tag="PWT25_000673"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL1020778.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="PWT25_000674"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="PWT25_000674"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL1020779.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="PWT25_000675"
     CDS             21391..21831
                     /locus_tag="PWT25_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL1020780.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="PWT25_000676"
     CDS             21834..22436
                     /locus_tag="PWT25_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL1020781.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="PWT25_000677"
     CDS             complement(22495..23601)
                     /locus_tag="PWT25_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL1020782.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="PWT25_000678"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="PWT25_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL1020783.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="PWT25_000679"
     CDS             complement(25149..27494)
                     /locus_tag="PWT25_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL1020784.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="PWT25_000680"
     CDS             27773..28675
                     /locus_tag="PWT25_000680"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL1020785.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="PWT25_000681"
     CDS             28888..30663
                     /locus_tag="PWT25_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL1020786.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="PWT25_000682"
     CDS             complement(30729..32315)
                     /locus_tag="PWT25_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL1020787.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="PWT25_000683"
     CDS             32566..32790
                     /locus_tag="PWT25_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL1020788.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="PWT25_000684"
     CDS             32932..34683
                     /locus_tag="PWT25_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1020789.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="PWT25_000685"
     CDS             34683..36467
                     /locus_tag="PWT25_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020790.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="PWT25_000686"
     CDS             36793..37644
                     /locus_tag="PWT25_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1020791.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="PWT25_000687"
     CDS             37698..39449
                     /locus_tag="PWT25_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1020792.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="PWT25_000688"
     CDS             39453..39623
                     /locus_tag="PWT25_000688"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1020793.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="PWT25_000689"
     CDS             complement(39663..40565)
                     /locus_tag="PWT25_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1020794.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="PWT25_000690"
     CDS             40796..42199
                     /locus_tag="PWT25_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL1020795.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="PWT25_000691"
     CDS             42199..43632
                     /locus_tag="PWT25_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL1020796.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="PWT25_000692"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="PWT25_000692"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL1020797.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="PWT25_000693"
     CDS             44917..46644
                     /locus_tag="PWT25_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL1020798.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="PWT25_000694"
     CDS             46659..47009
                     /locus_tag="PWT25_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL1020799.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="PWT25_000695"
     CDS             complement(47101..47961)
                     /locus_tag="PWT25_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL1020800.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="PWT25_000696"
     CDS             48053..48481
                     /locus_tag="PWT25_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL1020801.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /locus_tag="PWT25_000697"
     CDS             48643..48819
                     /locus_tag="PWT25_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL1020802.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="PWT25_000698"
     CDS             48846..49292
                     /locus_tag="PWT25_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL1020803.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="PWT25_000699"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="PWT25_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL1020804.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="PWT25_000700"
     CDS             51531..52502
                     /locus_tag="PWT25_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL1020805.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="PWT25_000701"
     CDS             52526..54718
                     /locus_tag="PWT25_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL1020806.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="PWT25_000702"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="PWT25_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL1020807.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="PWT25_000703"
     CDS             55195..55590
                     /locus_tag="PWT25_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL1020808.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="PWT25_000704"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="PWT25_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL1020809.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="PWT25_000705"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="PWT25_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL1020810.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="PWT25_000706"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="PWT25_000706"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL1020811.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /locus_tag="PWT25_000707"
     CDS             58762..60837
                     /locus_tag="PWT25_000707"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL1020812.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DANJLF010000010        60496 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000010 DANJLF010000000
VERSION     DANJLF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(12..1535)
                     /locus_tag="PWT25_000708"
     CDS             complement(12..1535)
                     /locus_tag="PWT25_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL1020813.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="PWT25_000709"
     CDS             1787..2968
                     /locus_tag="PWT25_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1020814.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="PWT25_000710"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="PWT25_000710"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL1020815.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="PWT25_000711"
     CDS             complement(5053..7764)
                     /locus_tag="PWT25_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020816.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="PWT25_000712"
     CDS             complement(7774..8475)
                     /locus_tag="PWT25_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020817.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="PWT25_000713"
     CDS             8661..9260
                     /locus_tag="PWT25_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1020818.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="PWT25_000714"
     CDS             9341..10354
                     /locus_tag="PWT25_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1020819.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="PWT25_000715"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="PWT25_000715"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL1020820.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="PWT25_000716"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="PWT25_000716"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL1020821.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="PWT25_000717"
     CDS             11802..13175
                     /locus_tag="PWT25_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1020822.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="PWT25_000718"
     CDS             13532..15676
                     /locus_tag="PWT25_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1020823.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="PWT25_000719"
     CDS             complement(15728..16768)
                     /locus_tag="PWT25_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL1020824.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="PWT25_000720"
     CDS             complement(17045..18847)
                     /locus_tag="PWT25_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020825.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="PWT25_000721"
     CDS             complement(18861..19613)
                     /locus_tag="PWT25_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020826.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="PWT25_000722"
     CDS             complement(19622..19984)
                     /locus_tag="PWT25_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1020827.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="PWT25_000723"
     CDS             complement(20160..21032)
                     /locus_tag="PWT25_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL1020828.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="PWT25_000724"
     CDS             complement(21058..21570)
                     /locus_tag="PWT25_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL1020829.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="PWT25_000725"
     CDS             complement(21708..22499)
                     /locus_tag="PWT25_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL1020830.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="PWT25_000726"
     CDS             complement(22554..23162)
                     /locus_tag="PWT25_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL1020831.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="PWT25_000727"
     CDS             complement(23155..23472)
                     /locus_tag="PWT25_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1020832.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="PWT25_000728"
     CDS             complement(23628..24356)
                     /locus_tag="PWT25_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL1020833.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="PWT25_000729"
     CDS             24515..25396
                     /locus_tag="PWT25_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1020834.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="PWT25_000730"
     CDS             complement(25633..26676)
                     /locus_tag="PWT25_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1020835.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="PWT25_000731"
     CDS             complement(26679..27347)
                     /locus_tag="PWT25_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1020836.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="PWT25_000732"
     CDS             27489..29825
                     /locus_tag="PWT25_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1020837.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="PWT25_000733"
     CDS             complement(29940..30251)
                     /locus_tag="PWT25_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020838.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="PWT25_000734"
     CDS             30432..31907
                     /locus_tag="PWT25_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL1020839.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="PWT25_000735"
     CDS             31954..32538
                     /locus_tag="PWT25_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL1020840.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="PWT25_000736"
     CDS             32540..32959
                     /locus_tag="PWT25_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL1020841.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="PWT25_000737"
     CDS             complement(33240..33524)
                     /locus_tag="PWT25_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL1020842.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="PWT25_000738"
     CDS             complement(33876..34586)
                     /locus_tag="PWT25_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL1020843.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="PWT25_000739"
     CDS             complement(34583..36055)
                     /locus_tag="PWT25_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL1020844.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="PWT25_000740"
     CDS             36193..36666
                     /locus_tag="PWT25_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1020845.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="PWT25_000741"
     CDS             complement(36819..37370)
                     /locus_tag="PWT25_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1020846.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="PWT25_000742"
     CDS             37475..38005
                     /locus_tag="PWT25_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL1020847.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="PWT25_000743"
     CDS             complement(38488..38931)
                     /locus_tag="PWT25_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1020848.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="PWT25_000744"
     CDS             complement(38947..40107)
                     /locus_tag="PWT25_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1020849.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="PWT25_000745"
     CDS             40614..42314
                     /locus_tag="PWT25_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020850.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="PWT25_000746"
     CDS             complement(42416..43249)
                     /locus_tag="PWT25_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL1020851.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="PWT25_000747"
     CDS             complement(43553..45718)
                     /locus_tag="PWT25_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1020852.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="PWT25_000748"
     CDS             45978..48272
                     /locus_tag="PWT25_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL1020853.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="PWT25_000749"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="PWT25_000749"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL1020854.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="PWT25_000750"
     CDS             48923..49930
                     /locus_tag="PWT25_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL1020855.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="PWT25_000751"
     CDS             49952..50971
                     /locus_tag="PWT25_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1020856.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="PWT25_000752"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="PWT25_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL1020857.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="PWT25_000753"
     CDS             complement(52729..54150)
                     /locus_tag="PWT25_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1020858.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="PWT25_000754"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="PWT25_000754"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL1020859.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="PWT25_000755"
     CDS             complement(56561..57817)
                     /locus_tag="PWT25_000755"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL1020860.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="PWT25_000756"
     CDS             58397..59698
                     /locus_tag="PWT25_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL1020861.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="PWT25_000757"
     CDS             complement(59767..60366)
                     /locus_tag="PWT25_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1020862.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18253 a        12374 c        10839 g        19030 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaa
//
LOCUS       DANJLF010000011        57249 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000011 DANJLF010000000
VERSION     DANJLF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            352..1041
                     /locus_tag="PWT25_000758"
     CDS             352..1041
                     /locus_tag="PWT25_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1020863.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="PWT25_000759"
     CDS             1028..2236
                     /locus_tag="PWT25_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1020864.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="PWT25_000760"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="PWT25_000760"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL1020865.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="PWT25_000761"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="PWT25_000761"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL1020866.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="PWT25_000762"
     CDS             complement(6339..7034)
                     /locus_tag="PWT25_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL1020867.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="PWT25_000763"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="PWT25_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL1020868.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="PWT25_000764"
     CDS             complement(8233..8424)
                     /locus_tag="PWT25_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL1020869.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="PWT25_000765"
     CDS             complement(8445..9335)
                     /locus_tag="PWT25_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL1020870.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="PWT25_000766"
     CDS             complement(9322..10089)
                     /locus_tag="PWT25_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1020871.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="PWT25_000767"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="PWT25_000767"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL1020872.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="PWT25_000768"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="PWT25_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL1020873.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="PWT25_000769"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="PWT25_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL1020874.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="PWT25_000770"
     CDS             14660..15043
                     /locus_tag="PWT25_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020875.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="PWT25_000771"
     CDS             complement(15211..15969)
                     /locus_tag="PWT25_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL1020876.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="PWT25_000772"
     CDS             complement(15987..16805)
                     /locus_tag="PWT25_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL1020877.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="PWT25_000773"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="PWT25_000773"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL1020878.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="PWT25_000774"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="PWT25_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL1020879.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="PWT25_000775"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="PWT25_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL1020880.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="PWT25_000776"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="PWT25_000776"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL1020881.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="PWT25_000777"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="PWT25_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL1020882.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="PWT25_000778"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="PWT25_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL1020883.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="PWT25_000779"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="PWT25_000779"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL1020884.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="PWT25_000780"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="PWT25_000780"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL1020885.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /locus_tag="PWT25_000781"
     CDS             26847..27146
                     /locus_tag="PWT25_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL1020886.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="PWT25_000782"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="PWT25_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1020887.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /locus_tag="PWT25_000783"
     CDS             27734..27970
                     /locus_tag="PWT25_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL1020888.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="PWT25_000784"
     CDS             28113..30086
                     /locus_tag="PWT25_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL1020889.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="PWT25_000785"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="PWT25_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL1020890.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="PWT25_000786"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="PWT25_000786"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL1020891.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="PWT25_000787"
     CDS             32965..34257
                     /locus_tag="PWT25_000787"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL1020892.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            34993..35856
                     /locus_tag="PWT25_000788"
     CDS             34993..35856
                     /locus_tag="PWT25_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1020893.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(35985..37607)
                     /locus_tag="PWT25_000789"
     CDS             complement(35985..37607)
                     /locus_tag="PWT25_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1020894.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            37856..38377
                     /locus_tag="PWT25_000790"
     CDS             37856..38377
                     /locus_tag="PWT25_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL1020895.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38563..40293)
                     /gene="spxB"
                     /locus_tag="PWT25_000791"
     CDS             complement(38563..40293)
                     /gene="spxB"
                     /locus_tag="PWT25_000791"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL1020896.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40458..41276
                     /locus_tag="PWT25_000792"
     CDS             40458..41276
                     /locus_tag="PWT25_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL1020897.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41379..41576)
                     /locus_tag="PWT25_000793"
     CDS             complement(41379..41576)
                     /locus_tag="PWT25_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020898.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41745..42323
                     /locus_tag="PWT25_000794"
     CDS             41745..42323
                     /locus_tag="PWT25_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL1020899.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42418..42555
                     /locus_tag="PWT25_000795"
     CDS             42418..42555
                     /locus_tag="PWT25_000795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020900.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43368..43871)
                     /locus_tag="PWT25_000796"
     CDS             complement(43368..43871)
                     /locus_tag="PWT25_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL1020901.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44230..45255
                     /locus_tag="PWT25_000797"
     CDS             44230..45255
                     /locus_tag="PWT25_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1020902.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45578..46456
                     /locus_tag="PWT25_000798"
     CDS             45578..46456
                     /locus_tag="PWT25_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL1020903.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            46932..47048
                     /locus_tag="PWT25_000799"
     CDS             46932..47048
                     /locus_tag="PWT25_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL1020904.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            47976..48314
                     /locus_tag="PWT25_000800"
     CDS             47976..48314
                     /locus_tag="PWT25_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020905.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48408..48638
                     /locus_tag="PWT25_000801"
     CDS             48408..48638
                     /locus_tag="PWT25_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020906.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49038..49409
                     /locus_tag="PWT25_000802"
     CDS             49038..49409
                     /locus_tag="PWT25_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL1020907.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49683..50375)
                     /locus_tag="PWT25_000803"
     CDS             complement(49683..50375)
                     /locus_tag="PWT25_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1020908.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50378..51163)
                     /locus_tag="PWT25_000804"
     CDS             complement(50378..51163)
                     /locus_tag="PWT25_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1020909.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51400..52800
                     /gene="lacG"
                     /locus_tag="PWT25_000805"
     CDS             51400..52800
                     /gene="lacG"
                     /locus_tag="PWT25_000805"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL1020910.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            52825..53142
                     /locus_tag="PWT25_000806"
     CDS             52825..53142
                     /locus_tag="PWT25_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL1020911.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53155..54828
                     /locus_tag="PWT25_000807"
     CDS             53155..54828
                     /locus_tag="PWT25_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL1020912.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            54842..56473
                     /locus_tag="PWT25_000808"
     CDS             54842..56473
                     /locus_tag="PWT25_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL1020913.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT        17626 a        10543 c        11396 g        17684 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    34381 cagtcccttt ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat
    34441 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    34501 gctttttcac aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca
    34561 caatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa
    34621 acggcaggct cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
    34681 caaaaaacgg caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca
    34741 aaacgctgat ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa
    34801 gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
    34861 ctgaccactt ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa
    34921 agtgtgtcta gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag
    34981 gggttgaaaa atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta
    35041 ttagcaaaag aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta
    35101 gtagatgacc tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt
    35161 gcgatgccta cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat
    35221 gcggaaaaac agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac
    35281 agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat
    35341 gtttttgata caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga
    35401 ttgaaaaaag aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg
    35461 aaagtccatt catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt
    35521 tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat
    35581 aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca
    35641 atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct
    35701 tacgctgggg atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag
    35761 atgaaagatg tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc
    35821 tgtatcgcga ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa
    35881 agcccagaaa ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc
    35941 tcttagaggt cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg
    36001 ttcaattgtt tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga
    36061 tcgatcacac ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga
    36121 tggtcatgtg acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct
    36181 gtgattgatt ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc
    36241 gaaagcatca tttttgaaag catgcaacgg actttctctc caccagataa aacattcact
    36301 ggtttcaata cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg
    36361 ttatcatctt cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta
    36421 tcgaattctt tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt
    36481 actgttccag tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt
    36541 atatcatttt ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca
    36601 tctaaaattt tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt
    36661 ccaatctcac gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata
    36721 tcgtctaaag taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat
    36781 gcattcgcac taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg
    36841 gcatttgcgt ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa
    36901 tttcctacat aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg
    36961 ttcaaaaagt gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat
    37021 tcttccagcc aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga
    37081 acatcaggtt taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt
    37141 aattcactca tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca
    37201 gctgcttctg gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct
    37261 gctcgaatac catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc
    37321 agcacttcat acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca
    37381 tagtcgtaat ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa
    37441 cctgtggaag gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca
    37501 tttgcaccaa tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat
    37561 agtttgcggt ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac
    37621 ttttatcatt attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt
    37681 caaggcaatc gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa
    37741 agcggagcga aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt
    37801 tatttataag agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga
    37861 tgaaaaaaga accatcgact ggggctcatt gattttggga attttatttg tacttgtctc
    37921 attgttatca tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat
    37981 ttttgccttt attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac
    38041 aggatataaa ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt
    38101 tttcttattc aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt
    38161 tattgctgat tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg
    38221 atattattgg tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt
    38281 gtttaatcca atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt
    38341 atttggtatc acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag
    38401 ccggcttccg attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa
    38461 atatgcacga ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat
    38521 atacacaaat tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata
    38581 taattcattc aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc
    38641 ttcttcagaa tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt
    38701 attgatcttc acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa
    38761 tcgaagttgc gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt
    38821 accgaaatct gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc
    38881 tgcttcgatg aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata
    38941 tttgacttgt gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt
    39001 aaatacttgt ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc
    39061 catagttgcg aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg
    39121 aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat
    39181 acgattgatt tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa
    39241 agatttcaac caatttaccc atttttcttt attttcttga tttgctaggt accagctatc
    39301 agcagggcga gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag
    39361 gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat
    39421 aaattcagca gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa
    39481 gatcagatcg gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa
    39541 accaagaaag ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac
    39601 aggaactgaa aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg
    39661 accaacataa agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc
    39721 tttttcttct ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc
    39781 gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc
    39841 ataggcggct ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt
    39901 acgattgtat acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt
    39961 atagttcatg gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat
    40021 ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac
    40081 agcgccgact ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc
    40141 atgacgaact tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga
    40201 attgaaagat cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt
    40261 taccattgcg attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga
    40321 tacataaatt ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt
    40381 cttttcacaa aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt
    40441 caagagaggg gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac
    40501 ttgctgggac agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct
    40561 ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga
    40621 ttgctgcaag tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag
    40681 atattgcttg gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg
    40741 cagataaaac actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta
    40801 cccatttaaa acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag
    40861 gtttagccgt gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta
    40921 tattagccgc tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag
    40981 ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac
    41041 aagcttctgg aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag
    41101 tggagctgct gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg
    41161 tggaagaatt gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt
    41221 ttggcgtcat gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa
    41281 aaaaaatata tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt
    41341 agtaagttat atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt
    41401 gtatttcctt tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg
    41461 accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct
    41521 gcaattagca ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa
    41581 aaccctcctc ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca
    41641 cctccaactg aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc
    41701 tctggtttat catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc
    41761 ttgaagcatt tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc
    41821 atgaacctaa cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct
    41881 atattgtcag ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag
    41941 ttgtacataa gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt
    42001 cgctttttcc gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa
    42061 tgacttatgg ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac
    42121 ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga
    42181 aatcgtatct atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc
    42241 cagtagccgt gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta
    42301 ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga
    42361 aaaaataaat aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg
    42421 gatcaaaaaa gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg
    42481 gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca
    42541 gcaataaaaa aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg
    42601 tttacagcag tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag
    42661 aaaagtgtat cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata
    42721 aaaataaaaa attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca
    42781 gaaatgaata aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa
    42841 aaaaataaca tatctcattt tacgcttttg ttattttata cttaaaaaat aacgacacag
    42901 aaaataccgt gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa
    42961 atgccttcaa aaagtaggat caggcagaat atactggcag aatcaaggag aagggagcct
    43021 aaataaggag aaaaaccata gaaaatataa agtaattact tataaaacaa gctttttata
    43081 gaaaaataat atacctgagt agggtataaa ttaagacgga attcctttct agattgagat
    43141 attgactagg aagaatgtta agattttaag cctttaagct aaaaaaaaac cctttttgtt
    43201 aaataagggg aatatagcct gtcagtttca aactgcaagt cgtcctgctg caataatata
    43261 gtagattaga gatgaaaata gattttttta aaccaatgac tgaataaaaa gaccgtcata
    43321 gatacacatt ggaatgtgtc tattacggtc tttttttacg aacgatttta ttgaagacgg
    43381 acagttcggg tcaaaaataa aaaagctacg atcagaaata agaataaaat gtatcccaaa
    43441 ataatggcta gacggtctaa cttatttgtc gatagatgtt tgtttagaag ttgataggcc
    43501 cataaaccta tcagtcctcc tgttgtatta gttatcaaat cagtgatgtc cgctacgcca
    43561 ataccgaata ggaattgaat caattctgcc aagaagctta caaggaaaat aatccctaat
    43621 tttttagaaa aagaaagtct cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag
    43681 acaatcaagt tataagtggt ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa
    43741 aaagggacga gattgacatt gctgtaagaa taagtgagaa attgtggaag attaaatgat
    43801 agcttgaata aaatcaacca taatagaaag aataaataaa tccctaataa aaattttgat
    43861 acataccgca tagacatctc ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat
    43921 cataaaaaag tatgaagttt aaggaccaac gtagttatcg tacgaaaatt agaagctttt
    43981 ttttgttggg aatccggttt ctttaacaaa gctaagatag cgagttgctt ggaaaagttg
    44041 ctatcttttt tctgtcaaaa cgactcggaa tgttcgtttt ttgttctaat ttctccaagt
    44101 tgtttacttt ttgatactaa gtttatgaaa aatcaagaaa tagttgttat actaggtaat
    44161 gctgacaaac gggattggca tttcatcttg gttcagcttt ttttccaatt actaggaagg
    44221 agggatagat tgttcaagac ttattttcgt ttcaataaaa tacaagataa tccttttcgt
    44281 atcatgaatc tgatgctact tatctttatt gtattatggt taggttacat aaatcatgat
    44341 tttttgattt caagtttttc atcattaggg atctttactt ttttctatta tcaaaacata
    44401 ccgatgaaat atttgctgaa acgtatgttt ctgatcggtt gcggactgtt ggttgctttc
    44461 gttttgggga tcttatcgac ttatgtttta tggcttgaac catttgccgt agctttggtt
    44521 gctttttcca gcagattcgt gttgcgatta ttccatattt ctaaaccagg tggcttgttt
    44581 tttgcgatgc tttctgccat gggaacgagc atgcagctgc caatcgctca attaccgatc
    44641 gtctcacttt atttttttat gggtgtcgtt tttgccctga ttgcggcagt gattacaaaa
    44701 ttactggatt cacggcctga gcagacaatt gaaaaagcaa ctttaaaaga aagatttcac
    44761 gaagagcctt tagtgatcat tgattctgtt ttctacagcg ctgccttatt tctttccgtt
    44821 tatgtcagtc acggcttgaa tctgcataat ccttattggt tgaccttgtc atgtgcatcg
    44881 attttgctag cagaaaactt ggatgcgatg aagcatcgac aagtccaata tctgattgga
    44941 tcgatgggtg ggttatgtgt gtcggcattc ttgtcatttg tgccgtttac gcagttgcag
    45001 accatttttc tgatcacttt tctatatgga atcgcacaat ttctagttgc tagaaattat
    45061 gcagttgcta atatttttct taatccgatg gcgttgatgc tatctacact tattcgtgga
    45121 gcatacttga tttctttgat cgaatatcgt tttttaggga tcgtcatagg aagtttcatc
    45181 ggtttaggtg ttgcgtgggt aatgacggtt ggtttgcaac attatttaac ggttgttcgt
    45241 caaaaattag aatagaagga caaatatatg tgccaaaaga ggttatgaca agatttttgt
    45301 cacaacctct aattcaaggt gttcaaaagc cagattctca gcattaagtc aaattcacac
    45361 aaaaatgtgg aaaggaactt tcaaaaaaat tttatattta ttctatcaaa aaattttgtt
    45421 tatgatggta actgaataaa ctttcctgag atggtcaatg ctttccttga aagtcttaca
    45481 gatattcttt cttcatagaa agtttaagtt ttatagttag tgtacaataa aagtatgttt
    45541 ataccatatg gtgtccggat aatcagtcat ttaaacaatg aggttcaaag atatgaaaag
    45601 gggaaatttg atgaagaaaa taagcaatga aatccaaatt ctggcagaaa ctttaactct
    45661 tcccaaagtc gttatagagc aaatgattgt acaagcaaaa aacatccttg atgaggtgtt
    45721 tgagcaatat agtcatgaac taatggatcc taataaagca aaggcgactg caaagaaaat
    45781 agacaaagtc tatgaagagg aaccttttgc agcattagct atttatctat atgcagcttc
    45841 tcaaagttgg aaaaaaattt atcaaccatt gaagattcca agaactatct atttggcaac
    45901 aatgaatgct tttactagat ttattcatga acatttccaa gtaacaggaa gctatcgctt
    45961 tgaccgtggt ttttggattt ggcattacac tagtggcttg atctttcgga ttggtgaatt
    46021 ggagtacgaa atgacatatt ttccgaaagg gtacacggat ctaagattag aaggaaagca
    46081 gtgcctttct cttcatattc cttccgatgc agatttatct cagaataaga taagagatag
    46141 ttacgaaagt gccattcgtt tttttgaact ttattttcct gattaccatt accaagtgat
    46201 gtacacagat acttggttac tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa
    46261 aatctgtctt tttgcagccg attatcgatt attatcagtt gatgaacagg acgatagtgg
    46321 agtcccttgg atctttggac gagtagatgc ccaaatacat gattatccag aaagtacgag
    46381 tttacagcga caagcaaaag agcaattgct tgcaggcgag cacattggct ctggcttagg
    46441 tattcttact tcataagttt agtacttcga aatagtttaa aagagcgtga gacaaaagta
    46501 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct
    46561 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    46621 ttcttggagt taaacgcttc tgtcccagcc tcttttttag tgttctatcg aacacacagt
    46681 acgttcaata ggtaaactag ctataacaat agatattaaa gttaacggac aagagggcct
    46741 tttttaggta gctaaaacgt aataatgaaa atttatttcc agagatagga gtcaatcagc
    46801 tagtctgatc gactgtttta acaagttgac tttcatttta acttttgata ccatttaaaa
    46861 cacaaatcgt aattgtttcg atttgtgttt tttagcatga ctacaatcaa agaagaaagt
    46921 gggtcgtcca tatggaaaaa acagatatct caagtgcata ccgccgcttg aaaagcccaa
    46981 atatcaaaac acgaaaacgt gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga
    47041 aaaggtaaga agaaggaaac ttaattaaaa aaatagccga aaaacgtttt tcggctattt
    47101 tcaataatcg gtttttgtgt aaggtttccg aaagaactgc tgttgatttt ccatttttaa
    47161 catatgataa attggtagaa tctttctttt caaattgata ccattcccta attccagtta
    47221 tatagttaac tacagaacca caaagcacgt acgtttataa aagtattatc tgtagttatt
    47281 tttcaattcc ctttcaacct cttattttga ccagtagtat tgatcacttt accttttaac
    47341 acctttgttc gtttttgaaa catttttagt caagaatgga aacgtaacta cataatcaaa
    47401 ggttttgcga aatatttcat gaaatatttc atgtagaagc gagcaatacg gttgcaaata
    47461 accattatcg tttaataagc agttacgaaa gctcccaata tctctaacgt tcagctcact
    47521 ccatttcttt tcttcaaaaa aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt
    47581 aactttaaaa agtaacgccg aaaaaatatg caaaaaaata aaaaagcagt tactattgct
    47641 agaataacat caaggaataa taagttttgg ttttcagata atagtcgata agttgtgtat
    47701 tggaatttta gatacagtaa taaagtattt ttcatgaaat tttgtgagaa tcactattac
    47761 aagtatcttt aataaaatta atggttaagg aggaacttaa agtagaatgt aaatgaatag
    47821 ttgtagtcag tcgattgtga tactagactc aaagttgcag gaataagttt ttcaatttgt
    47881 gattggaaga taaaaaatga aaaagaaggg ctttttttta tactttgttg attgtagtgt
    47941 ttattttccc tctgtttagc caagtaacat tttctatgac tcaaccagtt tcaagtgagg
    48001 aacatgataa cctttttcgt gctacagaag agtttaagaa atatcaggaa aatattgttc
    48061 cggagactcc attagctagt gagccaatta ctgatattga tggaaaccaa ataggatggt
    48121 atatacagta cgaagttatg gtggtaaata atgactcaat agtaaaaaat acagtaggct
    48181 tagagctaac accgattgaa aaggtgagtt cattgttaac gtatatttat actgatgaaa
    48241 gtgacgagac tgagatcatt ttaacggatt actcaaaaat tgctactgat aaaaagtata
    48301 tattgtggat gtagacacta gtgaaatact ttatgatata gaaattgatg aaaatttact
    48361 agatgtagca caagaaatcg atcatcaaaa gcaggaagct attcgatatg ctggtagaaa
    48421 agggaaaaga aaatatttat tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt
    48481 ggaaagaatt caagagtcgc tactcaatta actcgactcc gacatttaca gtttacagag
    48541 aaggatcgat agaaaaaact gttttttgga ctaaagaaag tggaatgtct ttagctgaag
    48601 tagaagagtt tttggattat gttagtatgc aacagtaagg gagttcttat tatgaaaaat
    48661 tttgacacat attatgttat tgttttttcg atatctttgc tgttaatatt actaacttta
    48721 atgcagtata aagagtatcc agagtatttt attttaactt ttgtatattt agtaagtagc
    48781 tacacacttc tatataaatt cagaagttct tctagagttt ctaaagtttg tttcttaatt
    48841 agcttattga ttctataata tttttgattt atataggaaa ttaaaaatat tatagaatca
    48901 acactcagag aacgggttgg tttttaatag atttaataag tactgataat gaaatttctc
    48961 ctggttggga aaacgaagtt ggttaccctg tagaagaatt ttttcaagat tactttgcgg
    49021 agtatgccga taggataatg agagattacg taacagataa aactcaagaa gtcatcaatc
    49081 aatcccagtc attaaatgaa ttatctgata aggcgaaaga agaagcagtt gccattattt
    49141 cttcagggat tagtgactat attgatatga gtggtgaaat caattatggc gataaagaca
    49201 aaattgtacg catgataaaa gcttttatca aaacttttga tgtgaaggca gatcttaagt
    49261 ttaatgatac ttacctagta ggaaaattaa ttaatatcct tgggacagag aaagatacgg
    49321 aaacgtttat ttcctctttg tctatgttgt ttacagataa gcatttttca actcagcttg
    49381 gtggagagga actagtggaa atcttttaac cattacgccc agcattaata aaagtatatc
    49441 aaaaaagtac tcctgatgat ttttaagatt ggtttgagaa ataatgtgaa aacactattt
    49501 ttaatgttaa ttatagatag agatgaacgc gtcatatggt taaaagaatg aaaaagtcaa
    49561 agatacactg ctatatttca ctaaggatat agcagtgtat ctttcatgtc gtttcactgg
    49621 cagtttttta taaaaggagg tgtgcatttc agttgctttt ctttggtagt tagttgaatc
    49681 gtttaagaat taacggatgt ggcagacagg tgctctgtcc aaaaaccagt aaaatctttg
    49741 atggttccat ctttatttag ctgtgcctgt tcaaatactg ttttgttttt accgaatgga
    49801 tcaattgcaa taattgttcc gatccccgca cgtttggcag caagtaatcc tgagtaagca
    49861 tcttcgataa caagacaatc agccggagct aagctcagtt tgtctgccgc tttaagaaaa
    49921 atatccggtt gcggtttccc agggaaagag ccgtcgtcat agacaatctt gtcccagtca
    49981 aaccagcggg ctaaatcgaa aatatcaaaa taaaaagcaa cattctcttt gacagtagcc
    50041 gtggcaatcg ttaatggaat ctgttggtca gtcagctggt caagagtcgc ttcaagccct
    50101 tttgtcaact gaagctcgtt ttcattttgc aagcaaagca tacgataata gctttctttt
    50161 tcttctgaca atacggcgat ctcagaggcg gtcaaatgct ctgaaatgaa atgtttcaaa
    50221 atctccgtat tcgtccgacc atgaatattt tttaaaatat cctcgtctga taaaccatct
    50281 tttgtatgat catgtatcat atgcagccaa gcagcttcat gcagatgaga gtccaaaaac
    50341 attgttccgt tgaaatcaaa tataatccct ttcatagtta cgctcctata gtggtatttt
    50401 cggttgaatc agcaatttgc agatcgacat attgtgcata gtgctcttga acctcccgcg
    50461 agatctggcg atccgtaatg agcagatcga aatcatgcaa attataaaac acataaaaat
    50521 catctcgatt gaatttttta ttatcaacga gtagatattt cttacgggaa ttatttagag
    50581 cgatctgctg tgcttctcct tcttcggtac tgtaagtaga gatcgcttcg ttgtgaacgc
    50641 catttgaact gataaatgct tttgtaaact ttagtcttct taaattgtcg ttggtgatcg
    50701 gtccgacaaa tgcgccggta tttttccgga aatcaccgcc aatcagtaga atttctggtc
    50761 catctgcttg agcaagaata tcaaaaactg gataattatt tgtaatgatc cgaattttct
    50821 tgttcagtaa atgcgtcgcc aatagttcga ttgttgtacc tggtcctaag aaaatcgttt
    50881 ctccctcgtg aatcaaggag gctgctgtgg cagcaatttc ttttttttct tgaatctgca
    50941 gagtggcttt ttcagtgtga gaaagttctt gatctaaagt gtaggaaaga ctttgagccc
    51001 ctccatggac acgcagcagc ttgcccgatt tttctaattc atctaaatct ctgcgaacgg
    51061 tcatatcaga aacatctaat tgattgatga tttcattaac ggtaaggatt ccttttttat
    51121 tgaccatttc tacgatagta agcaatcttt cttttttcaa cattgtcttc acctctaatt
    51181 taaggataat ctatcatata aacggacaac agtcaatcta attcgaacaa aagtaaacat
    51241 ttagggtggt ttttatgttt gttacaatag attttattgc tgttttctgg tttttatgat
    51301 tatttgtgtt tgtttttgtt gacaaatgtt ttggatgcgt ttacaatgca atcagttaga
    51361 aagagcgaaa actgattata gatttgagga gagatgtaca tgcttaaatt accagaagat
    51421 tttatttttg gcggagccac agcagcctat caggttgaag gagccactaa ggaaggtggc
    51481 aaaggcgctg ttgcttggga tgacttttta gaggaacaag ggcgattcag tcccgatccg
    51541 gcaagtgatt tttatcatca atatgcaaag gacattgaat tgtgtgaacg ttttggtgta
    51601 aatggtctaa gattatccat tgcttggagc cgcatttttc ctgatggagc tgggaaacca
    51661 aatccagaag gaatcgcttt ttatcatcgt gtgttcgaag aatgtaagaa aagaaacgta
    51721 acgccgtttg tgacgttgca tcattttgat acccctaaac gtttgtttga ccatggagat
    51781 tttttgaatc gagaaacaat agaagcattt gtttcctatg ccattttttg ttttcatgaa
    51841 ttcaaagaag tcaaggtgtg gagtaccttt aatgaaatct atccagttgc aaccaatcaa
    51901 tatttgttag gtgttttccc accaggaatc aaatatgatt ttacaaagat cgtcgcctgt
    51961 cttcataata tgatggttgc tcatgcccgt gtagtgaact atttcaaaga aaacgaactt
    52021 ccgggtgaaa ttggtgtagt tcactcatta gagacgaaat atgcggcaac agacgcgccg
    52081 gaagataagc atgctgcctt tttagacgat gcattgtcga tccgtttctt attagatgct
    52141 acctatttag gctattattc aactgaaacc ttgactgctc ttgatgagat atgtgaagca
    52201 aatcaggcca gctatcattt tcctgaagaa gatttcgttg aattgaaaaa agcatccacg
    52261 cgtaatgact acttagggat caatcactac caatgccatt ttgtcaaagc ttacgatgga
    52321 gaaaatgcaa tccatcataa cggtacgggt gaaaaaggga cctctgttta taaagtgaag
    52381 ggaatcggag aacgtattta taaagaagga attcctcgaa ctgattggga ctggctgatt
    52441 tatccagaag gtctatatga cttgttgctg cgaattaaaa gcgattaccc acattacaac
    52501 aaaatctata tcactgagaa cgggatgggg tataaagatc agtttgaaga tggcattatt
    52561 atggatcagc cgagaatcga ctatttgaga gtctatttgg aatccttaag caaggcgata
    52621 acagccgggg taaatgtgaa ggggtatttt ttgtggtcgc ttatggatct tttctcatgg
    52681 acaaatgggt ataacaaaag gtatggcttg ttttatgttg attttgaaac ccagaagcgt
    52741 tacccaaaag aatcagctta ctggtacaag ttggtcagtg aaaccaaaac gattatttaa
    52801 taagtgataa tggaggaaga aaagatgaat aaagaggaat tacaaatgct tggttttgag
    52861 atcgttgcat attcaggcga cgctcgcagt acattattga aattattgaa agaagtaaga
    52921 caaggaaact ttgaacatgt tgattcggcg ttgaaagaag ccgacgaaaa tttaaccctt
    52981 gcacataatt cgcaaacaaa aattttagcg gaagaagcat ctgggaaaga gatggagatg
    53041 ggattcattt ttatccatgg gcaggatcat ttaatgacca ctttattgct gagagatctg
    53101 attcaagact ttattgtact gtacagacag aatcaaggat aggagaagaa cgctatggat
    53161 ggaatcatta aacaaatcga aaaagggaaa ccattttttg aaaaaatctc acgtaatagt
    53221 tatttaggag ccgttcgtga cggattttta acagcgatgc cagctatttt attttcaagc
    53281 attttcattt tgattgctgc ggttcctgag atttttggtt accaatggcc tgaatctgtc
    53341 tccacatggc tatggaaagt ctataattat tcgatgggtg ttgtagggtt actcgtttca
    53401 gcaaccactg ctcgctgctt ggctgattcg atgaatcgtc gcttaccgaa aaacaaagta
    53461 atcaacacca cctctgtgat gctggcctct gtggtaagtt tcctgttgtt aagcgtctct
    53521 ccgattgaag gtggattttc aacggaatat atggggacaa agggactact ggcttccttt
    53581 gtcgctgcat ttttaacagt taatttatat aagttttgtg tgctgaaaga tattaccatt
    53641 aaaatgccta aagaagttcc tggaacgatt tcgcaaacct tccgagatat ttttcctttc
    53701 tctttttcag tatttgcggc cgttttaatt gatttaggca ttcgacaaat cttccaaatc
    53761 tcatttgcag aagcaatcat tacggtgctg cagccgctat ttacagcagc tgacgggtac
    53821 ttaggaatcg ctattatctg gggagcgatg gcgctgtttt ggtttgtcgg tgttcatgga
    53881 ccgtcgatcg tggagccggc aattgcggca attatctatg ccaatgtgga aaccaatctg
    53941 caattgttca attccggtga acaagcttct aaagtactga cggtgggttt agggaatttt
    54001 gtaggaacta tgggaggaac tggggcaaca ttggttgttc cttttatctt cctactcttt
    54061 gctaagtcta agcagttgaa ggcagttggg aaagcttcct ttattccagt aagtttcgct
    54121 gtcaacgaac cattgctatt tgcggcaccc atgattttga acccttattt ttttattcca
    54181 tttttgttaa cacctgttgt aaacgtttgc ctgttcaagt tttttgtcga tgtattgggc
    54241 atgaacagct ttatgtacgt tttgccttgg gcaactccag caccgatcgg tttggttcta
    54301 ggaacgggca tgggtatctt agcttttgtt ctagcagcgg ttctaatcgt cgttgacttc
    54361 ctgatctatc taccattttg caaagcatac gatgctgtct tagtagagaa agaagcacaa
    54421 aaagcagagg tgatcgcaac cgaggaaggt gcttctgccg atgtacgtga ggaagtacct
    54481 gtggcagcga gtcaagttat cagcgatgca aataaagaaa tcaatgtgtt ggtactttgt
    54541 gctggcgcag ggacaagtgc gatgttagct aatgctttaa aaaaaggtgc atctgaatcg
    54601 aaagcaccga tcagtgcaat ggcgggtgct tatggttctc attacgaaat gctgcaggat
    54661 tttgatatgg tggtcttagc accgcaagtc aattcttact atgatgatat caaagaagac
    54721 accgatcgct taggaatcaa gctggttgcg acaaaaggag cagaatatat cggattaacg
    54781 agagatcctg aaaaagctgt tcagtttatt ctgaaccaat ttcaataaag gaggagccat
    54841 aatgaaaatg ggaatttggt tgcgtaaagc tgttttattt gcagctgtgt taggggcttt
    54901 gggggctgtt tcaaaaactg ttgaagcatc tgccagcgaa tttattcgcg gagcagatat
    54961 ttcgatcctg tcagatatgg aaaaatctgg tgcaaaatat tatgaagacg atatttcaaa
    55021 agatgcctta gagattttaa aaaataatgg tgtcaactat gttcgactgc gtttgtggca
    55081 ggatccttat aatagtaatg gagaaagtta tggagcaggg acaaatgatc tggaaacgac
    55141 gattgacctt gcaaaacgag caaaaaatct gggacttaag gtgctgctgg attttcacta
    55201 cagtgatttt tgggttgatc ctgggaaaca gaatcttcct aaagcatggc aaggtctgac
    55261 ctttgaagaa atgaatacgg cattgtacga ctatacaaag aacgttctgt cggaaatgaa
    55321 gcagctggac gtttatcctg atatggttca gatcggcaat gaactgaata gcggaatgct
    55381 ctggccttat ggcaagtctt ggggagaagg cgggggagaa tttgatcgat tggctgcttt
    55441 tttgaagtct ggtatccaag ctgttcgaga cactcaacct aaaacaactc cagtaatgct
    55501 ccacttagct gatggtggcg atacaggagc atttacttgg tggtttgatg aaatcacgag
    55561 tcgaggtgtg tcatttgatt taattggagt gtcgtattat ccgtattggc acggttcaat
    55621 gaatgaactt caaaccaata tgaatgcgat cagccaacgg tatggtaaag aagtgatcgt
    55681 agttgaaaca gcttatgggc atacaacagc gaatgccgat actatgccaa atgcttttgg
    55741 tgaagccgaa gcagctgcgg ggggatatga accaacaccg acaggacaag cagagtattt
    55801 gctggatctt gccgatcgga ttcaggcagt accgaataat cgcggggcgg gcttcttcta
    55861 ttgggaacct ctttggtaca acggaaatgt gagttgggca acacaagcag gtatgaatta
    55921 tctcagtgtt caatccacta tgggaaacga atgggacaac caagcgatgt ttgattttgc
    55981 tggaaacgcc ttgccaagct tgcgtgcgtt taaacaagca ggggcgcaaa taaatctagt
    56041 taaaaatgca agtttcgaaa gcgatggacc aacgaccagt ccttccaatt ggcaaacctg
    56101 gtttcaacaa ggaaccgcta agggtacgat caaaacggaa gcgggcactt tttttgggga
    56161 ctacaaacta actttttggc aggatcaagc atacgaggca tcagcttatc aaaatgttag
    56221 tggcataagc aaaggaactt acacattgag tgcctatgta atgtctgggg gagagtttga
    56281 tactaatcag ttatatatca aaaattatgg tggagcggaa ctaaatactg cggttgttaa
    56341 aacaagtgaa tggacaaaaa ttacaatcaa agacattcca attacaacag gtgtttgtga
    56401 aattggggtt tatagcaagg cttcaggaaa tgcttggacg aacgttgatt ttgtttcatt
    56461 ggtgaaacaa tagactgcgg atcgtttcga ttcaagatcg gaggttccct tcagccacat
    56521 cgttagcgta gaaggcagga atcgaacctg cacctcgcct ttgccaaaaa taaccagaaa
    56581 cgatttccag tcatttttac caaaacgagt aaagcaccag acttcatcta cactttgtat
    56641 tatgctaaag agagttcaac gatttcgtaa gaagcaatga aaaaattgca aaaagttgtg
    56701 cagataacat aaagtttgtt taagaaaggg taaccttaga attccgtcgt gaatatgatt
    56761 tgagcactta gatggataga aaaaagttct cggaaaacag ttcgtttttc gagaacttct
    56821 ttttatgata agaatgaaac aaattaactt atattatagt ctcttttggt agggaaccat
    56881 cttattttta atgaaaatca agcattttag ctattgaaga atgttgattt atagggattc
    56941 cccctccata ttatgttggc tctttattcc cactctgttt tcaacgtaaa gtgtataaaa
    57001 cagaatgcga tactatcaag ggagttaaaa caatatacag aacagtaaaa cactataaat
    57061 aaaaataaaa gtgcaataaa agtgcaattt ttgcacctaa tcatactctg tttatatatg
    57121 aaaactagat tctgaatcca atatttggtt aacgagaggc gttcgaattt gaacgtcttt
    57181 tatatattac tttcaaataa atatgaggta gaatactatc tattttcttt ttaacccaat
    57241 tgttataat
//
LOCUS       DANJLF010000012        51059 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000012 DANJLF010000000
VERSION     DANJLF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            330..1175
                     /locus_tag="PWT25_000809"
     CDS             330..1175
                     /locus_tag="PWT25_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1020914.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="PWT25_000810"
     CDS             1456..2676
                     /locus_tag="PWT25_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL1020915.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="PWT25_000811"
     CDS             2673..5393
                     /locus_tag="PWT25_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1020916.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="PWT25_000812"
     CDS             5377..6321
                     /locus_tag="PWT25_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1020917.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="PWT25_000813"
     CDS             complement(6390..7400)
                     /locus_tag="PWT25_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1020918.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="PWT25_000814"
     CDS             complement(7580..7924)
                     /locus_tag="PWT25_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL1020919.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="PWT25_000815"
     CDS             complement(7917..8351)
                     /locus_tag="PWT25_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL1020920.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="PWT25_000816"
     CDS             8489..9226
                     /locus_tag="PWT25_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020921.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="PWT25_000817"
     CDS             9219..10430
                     /locus_tag="PWT25_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020922.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="PWT25_000818"
     CDS             10634..11416
                     /locus_tag="PWT25_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL1020923.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="PWT25_000819"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="PWT25_000819"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL1020924.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="PWT25_000820"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="PWT25_000820"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL1020925.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="PWT25_000821"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="PWT25_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL1020926.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="PWT25_000822"
     CDS             15018..16067
                     /locus_tag="PWT25_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL1020927.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="PWT25_000823"
     CDS             16064..16342
                     /locus_tag="PWT25_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL1020928.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="PWT25_000824"
     CDS             16317..16787
                     /locus_tag="PWT25_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL1020929.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="PWT25_000825"
     CDS             16804..17106
                     /locus_tag="PWT25_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020930.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="PWT25_000826"
     CDS             17123..17494
                     /locus_tag="PWT25_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL1020931.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="PWT25_000827"
     CDS             17466..17825
                     /locus_tag="PWT25_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020932.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="PWT25_000828"
     CDS             17997..19004
                     /locus_tag="PWT25_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1020933.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="PWT25_000829"
     CDS             19029..20213
                     /locus_tag="PWT25_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL1020934.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="PWT25_000830"
     CDS             complement(20650..22233)
                     /locus_tag="PWT25_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1020935.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="PWT25_000831"
     CDS             complement(22811..22954)
                     /locus_tag="PWT25_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL1020936.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="PWT25_000832"
     CDS             complement(23176..23655)
                     /locus_tag="PWT25_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1020937.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="PWT25_000833"
     CDS             complement(23728..24012)
                     /locus_tag="PWT25_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020938.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="PWT25_000834"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWT25_000834"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="PWT25_000835"
     CDS             complement(24356..25642)
                     /locus_tag="PWT25_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL1020939.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="PWT25_000836"
     CDS             25780..26451
                     /locus_tag="PWT25_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL1020940.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="PWT25_000837"
     CDS             26417..26905
                     /locus_tag="PWT25_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL1020941.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="PWT25_000838"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="PWT25_000838"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL1020942.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="PWT25_000839"
     CDS             27866..28630
                     /locus_tag="PWT25_000839"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL1020943.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="PWT25_000840"
     CDS             28863..31076
                     /locus_tag="PWT25_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL1020944.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="PWT25_000841"
     CDS             31087..31533
                     /locus_tag="PWT25_000841"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL1020945.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="PWT25_000842"
     CDS             31704..32963
                     /locus_tag="PWT25_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL1020946.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="PWT25_000843"
     CDS             complement(33052..33999)
                     /locus_tag="PWT25_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1020947.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="PWT25_000844"
     CDS             34393..35685
                     /locus_tag="PWT25_000844"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL1020948.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="PWT25_000845"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="PWT25_000845"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL1020949.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="PWT25_000846"
     CDS             37578..38453
                     /locus_tag="PWT25_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1020950.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="PWT25_000847"
     CDS             38719..40602
                     /locus_tag="PWT25_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL1020951.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="PWT25_000848"
     CDS             complement(40677..40961)
                     /locus_tag="PWT25_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL1020952.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="PWT25_000849"
     CDS             complement(40951..41685)
                     /locus_tag="PWT25_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL1020953.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="PWT25_000850"
     CDS             41814..42437
                     /locus_tag="PWT25_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL1020954.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="PWT25_000851"
     CDS             42629..43750
                     /locus_tag="PWT25_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL1020955.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="PWT25_000852"
     CDS             43757..46885
                     /locus_tag="PWT25_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL1020956.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="PWT25_000853"
     CDS             46904..50098
                     /locus_tag="PWT25_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL1020957.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="PWT25_000854"
     CDS             complement(50244..50951)
                     /locus_tag="PWT25_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1020958.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16712 a         8924 c        10614 g        14809 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatat
//
LOCUS       DANJLF010000013        45086 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000013 DANJLF010000000
VERSION     DANJLF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            212..604
                     /locus_tag="PWT25_000855"
     CDS             212..604
                     /locus_tag="PWT25_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1020959.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="PWT25_000856"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="PWT25_000856"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL1020960.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="PWT25_000857"
     CDS             3165..4775
                     /locus_tag="PWT25_000857"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL1020961.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="PWT25_000858"
     CDS             5067..5936
                     /locus_tag="PWT25_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL1020962.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="PWT25_000859"
     CDS             6101..7372
                     /locus_tag="PWT25_000859"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL1020963.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="PWT25_000860"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="PWT25_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL1020964.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="PWT25_000861"
     CDS             8763..9026
                     /locus_tag="PWT25_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL1020965.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="PWT25_000862"
     CDS             9471..9920
                     /locus_tag="PWT25_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1020966.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="PWT25_000863"
     CDS             9977..11395
                     /locus_tag="PWT25_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1020967.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="PWT25_000864"
     CDS             11395..12255
                     /locus_tag="PWT25_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL1020968.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="PWT25_000865"
     CDS             12429..13202
                     /locus_tag="PWT25_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1020969.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="PWT25_000866"
     CDS             13349..13714
                     /locus_tag="PWT25_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL1020970.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="PWT25_000867"
     CDS             complement(13862..15070)
                     /locus_tag="PWT25_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1020971.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="PWT25_000868"
     CDS             complement(15089..15367)
                     /locus_tag="PWT25_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1020972.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="PWT25_000869"
     CDS             15521..16216
                     /locus_tag="PWT25_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL1020973.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="PWT25_000870"
     CDS             complement(16658..17455)
                     /locus_tag="PWT25_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL1020974.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="PWT25_000871"
     CDS             complement(17452..18636)
                     /locus_tag="PWT25_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL1020975.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="PWT25_000872"
     CDS             complement(18661..19908)
                     /locus_tag="PWT25_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL1020976.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="PWT25_000873"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="PWT25_000873"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL1020977.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="PWT25_000874"
     CDS             21888..22202
                     /locus_tag="PWT25_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL1020978.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="PWT25_000875"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="PWT25_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL1020979.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="PWT25_000876"
     CDS             22887..23531
                     /locus_tag="PWT25_000876"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL1020980.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="PWT25_000877"
     CDS             23546..23875
                     /locus_tag="PWT25_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL1020981.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="PWT25_000878"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="PWT25_000878"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL1020982.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="PWT25_000879"
     CDS             24863..25687
                     /locus_tag="PWT25_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL1020983.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="PWT25_000880"
     CDS             25680..26027
                     /locus_tag="PWT25_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL1020984.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="PWT25_000881"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="PWT25_000881"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL1020985.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="PWT25_000882"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="PWT25_000882"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL1020986.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWT25_000883"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWT25_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL1020987.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWT25_000884"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWT25_000884"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL1020988.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="PWT25_000885"
     CDS             31657..32451
                     /locus_tag="PWT25_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1020989.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="PWT25_000886"
     CDS             32441..34483
                     /locus_tag="PWT25_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1020990.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="PWT25_000887"
     CDS             34764..34913
                     /locus_tag="PWT25_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL1020991.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="PWT25_000888"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="PWT25_000888"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL1020992.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="PWT25_000889"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="PWT25_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL1020993.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="PWT25_000890"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="PWT25_000890"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL1020994.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="PWT25_000891"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="PWT25_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL1020995.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="PWT25_000892"
     CDS             39330..40412
                     /locus_tag="PWT25_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL1020996.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="PWT25_000893"
     CDS             40412..41215
                     /locus_tag="PWT25_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL1020997.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="PWT25_000894"
     CDS             41285..42025
                     /locus_tag="PWT25_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1020998.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="PWT25_000895"
     CDS             42036..43040
                     /locus_tag="PWT25_000895"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL1020999.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="PWT25_000896"
     CDS             complement(43085..44929)
                     /locus_tag="PWT25_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021000.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14522 a         7846 c         9369 g        13349 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaa
//
LOCUS       DANJLF010000014        44939 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000014 DANJLF010000000
VERSION     DANJLF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(38..1189)
                     /locus_tag="PWT25_000897"
     CDS             complement(38..1189)
                     /locus_tag="PWT25_000897"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL1021001.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="PWT25_000898"
     CDS             complement(1202..1639)
                     /locus_tag="PWT25_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1021002.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="PWT25_000899"
     CDS             complement(1654..3420)
                     /locus_tag="PWT25_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL1021003.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="PWT25_000900"
     CDS             3684..4361
                     /locus_tag="PWT25_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1021004.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="PWT25_000901"
     CDS             4364..5491
                     /locus_tag="PWT25_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021005.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="PWT25_000902"
     CDS             complement(5691..6131)
                     /locus_tag="PWT25_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1021006.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="PWT25_000903"
     CDS             complement(6142..8175)
                     /locus_tag="PWT25_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1021007.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="PWT25_000904"
     CDS             complement(8177..9880)
                     /locus_tag="PWT25_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1021008.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="PWT25_000905"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="PWT25_000905"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL1021009.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="PWT25_000906"
     CDS             complement(10963..11988)
                     /locus_tag="PWT25_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL1021010.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="PWT25_000907"
     CDS             complement(12197..13066)
                     /locus_tag="PWT25_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL1021011.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="PWT25_000908"
     CDS             complement(13525..15417)
                     /locus_tag="PWT25_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1021012.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="PWT25_000909"
     CDS             complement(15414..17141)
                     /locus_tag="PWT25_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1021013.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="PWT25_000910"
     CDS             complement(17157..17807)
                     /locus_tag="PWT25_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL1021014.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="PWT25_000911"
     CDS             18011..18307
                     /locus_tag="PWT25_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL1021015.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="PWT25_000912"
     CDS             18479..19099
                     /locus_tag="PWT25_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL1021016.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="PWT25_000913"
     CDS             complement(19191..20357)
                     /locus_tag="PWT25_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1021017.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="PWT25_000914"
     CDS             complement(20369..20722)
                     /locus_tag="PWT25_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL1021018.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="PWT25_000915"
     CDS             20850..21872
                     /locus_tag="PWT25_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL1021019.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="PWT25_000916"
     CDS             21891..22052
                     /locus_tag="PWT25_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021020.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="PWT25_000917"
     CDS             complement(22311..22745)
                     /locus_tag="PWT25_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021021.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="PWT25_000918"
     CDS             complement(22855..24714)
                     /locus_tag="PWT25_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL1021022.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="PWT25_000919"
     CDS             complement(24832..25992)
                     /locus_tag="PWT25_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL1021023.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="PWT25_000920"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="PWT25_000920"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL1021024.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="PWT25_000921"
     CDS             complement(27739..28437)
                     /locus_tag="PWT25_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL1021025.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="PWT25_000922"
     CDS             complement(28394..30055)
                     /locus_tag="PWT25_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL1021026.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="PWT25_000923"
     CDS             30273..31673
                     /locus_tag="PWT25_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL1021027.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="PWT25_000924"
     CDS             complement(31925..33004)
                     /locus_tag="PWT25_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1021028.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="PWT25_000925"
     CDS             complement(33090..34049)
                     /locus_tag="PWT25_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL1021029.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="PWT25_000926"
     CDS             complement(34061..35572)
                     /locus_tag="PWT25_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL1021030.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="PWT25_000927"
     CDS             complement(35594..36157)
                     /locus_tag="PWT25_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL1021031.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="PWT25_000928"
     CDS             complement(36163..37026)
                     /locus_tag="PWT25_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1021032.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="PWT25_000929"
     CDS             complement(37040..37936)
                     /locus_tag="PWT25_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1021033.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="PWT25_000930"
     CDS             complement(38111..39400)
                     /locus_tag="PWT25_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1021034.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="PWT25_000931"
     CDS             complement(39372..40889)
                     /locus_tag="PWT25_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL1021035.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="PWT25_000932"
     CDS             41087..41977
                     /locus_tag="PWT25_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL1021036.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="PWT25_000933"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="PWT25_000933"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL1021037.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="PWT25_000934"
     CDS             complement(42886..44358)
                     /locus_tag="PWT25_000934"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL1021038.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14780 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgttttt
//
LOCUS       DANJLF010000015        43607 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000015 DANJLF010000000
VERSION     DANJLF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            14..700
                     /locus_tag="PWT25_000935"
     CDS             14..700
                     /locus_tag="PWT25_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL1021039.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            702..1646
                     /locus_tag="PWT25_000936"
     CDS             702..1646
                     /locus_tag="PWT25_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL1021040.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1845..2489
                     /gene="pcp"
                     /locus_tag="PWT25_000937"
     CDS             1845..2489
                     /gene="pcp"
                     /locus_tag="PWT25_000937"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL1021041.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2568..3845
                     /locus_tag="PWT25_000938"
     CDS             2568..3845
                     /locus_tag="PWT25_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021042.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4029..4778)
                     /locus_tag="PWT25_000939"
     CDS             complement(4029..4778)
                     /locus_tag="PWT25_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL1021043.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            5108..5488
                     /locus_tag="PWT25_000940"
     CDS             5108..5488
                     /locus_tag="PWT25_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1021044.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            5584..6093
                     /locus_tag="PWT25_000941"
     CDS             5584..6093
                     /locus_tag="PWT25_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021045.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            6170..6349
                     /locus_tag="PWT25_000942"
     CDS             6170..6349
                     /locus_tag="PWT25_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021046.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            6374..6967
                     /locus_tag="PWT25_000943"
     CDS             6374..6967
                     /locus_tag="PWT25_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1021047.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(7014..7529)
                     /locus_tag="PWT25_000944"
     CDS             complement(7014..7529)
                     /locus_tag="PWT25_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021048.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(7555..8424)
                     /locus_tag="PWT25_000945"
     CDS             complement(7555..8424)
                     /locus_tag="PWT25_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1021049.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8550..9230
                     /locus_tag="PWT25_000946"
     CDS             8550..9230
                     /locus_tag="PWT25_000946"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL1021050.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9383..10363
                     /gene="pta"
                     /locus_tag="PWT25_000947"
     CDS             9383..10363
                     /gene="pta"
                     /locus_tag="PWT25_000947"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL1021051.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            10486..10959
                     /gene="tsaE"
                     /locus_tag="PWT25_000948"
     CDS             10486..10959
                     /gene="tsaE"
                     /locus_tag="PWT25_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL1021052.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            10952..11476
                     /locus_tag="PWT25_000949"
     CDS             10952..11476
                     /locus_tag="PWT25_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1021053.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(11966..12499)
                     /locus_tag="PWT25_000950"
     CDS             complement(11966..12499)
                     /locus_tag="PWT25_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL1021054.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(13363..14115)
                     /gene="xth"
                     /locus_tag="PWT25_000951"
     CDS             complement(13363..14115)
                     /gene="xth"
                     /locus_tag="PWT25_000951"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL1021055.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            14228..15148
                     /locus_tag="PWT25_000952"
     CDS             14228..15148
                     /locus_tag="PWT25_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1021056.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            15221..16156
                     /gene="murB"
                     /locus_tag="PWT25_000953"
     CDS             15221..16156
                     /gene="murB"
                     /locus_tag="PWT25_000953"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL1021057.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            16454..17932
                     /locus_tag="PWT25_000954"
     CDS             16454..17932
                     /locus_tag="PWT25_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021058.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            18209..20269
                     /locus_tag="PWT25_000955"
     CDS             18209..20269
                     /locus_tag="PWT25_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL1021059.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            20352..20921
                     /locus_tag="PWT25_000956"
     CDS             20352..20921
                     /locus_tag="PWT25_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1021060.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(21096..21902)
                     /locus_tag="PWT25_000957"
     CDS             complement(21096..21902)
                     /locus_tag="PWT25_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021061.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            22040..23554
                     /locus_tag="PWT25_000958"
     CDS             22040..23554
                     /locus_tag="PWT25_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL1021062.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(23957..24940)
                     /locus_tag="PWT25_000959"
     CDS             complement(23957..24940)
                     /locus_tag="PWT25_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL1021063.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(24945..25592)
                     /locus_tag="PWT25_000960"
     CDS             complement(24945..25592)
                     /locus_tag="PWT25_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1021064.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(25677..26309)
                     /locus_tag="PWT25_000961"
     CDS             complement(25677..26309)
                     /locus_tag="PWT25_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1021065.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(26325..26876)
                     /gene="coaC"
                     /locus_tag="PWT25_000962"
     CDS             complement(26325..26876)
                     /gene="coaC"
                     /locus_tag="PWT25_000962"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL1021066.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(26873..27610)
                     /locus_tag="PWT25_000963"
     CDS             complement(26873..27610)
                     /locus_tag="PWT25_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL1021067.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(27607..28596)
                     /locus_tag="PWT25_000964"
     CDS             complement(27607..28596)
                     /locus_tag="PWT25_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL1021068.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            28854..29396
                     /locus_tag="PWT25_000965"
     CDS             28854..29396
                     /locus_tag="PWT25_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1021069.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            29413..30498
                     /locus_tag="PWT25_000966"
     CDS             29413..30498
                     /locus_tag="PWT25_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1021070.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            30510..31316
                     /locus_tag="PWT25_000967"
     CDS             30510..31316
                     /locus_tag="PWT25_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021071.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            31317..32144
                     /locus_tag="PWT25_000968"
     CDS             31317..32144
                     /locus_tag="PWT25_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021072.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            32141..33214
                     /locus_tag="PWT25_000969"
     CDS             32141..33214
                     /locus_tag="PWT25_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1021073.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            33642..36338
                     /locus_tag="PWT25_000970"
     CDS             33642..36338
                     /locus_tag="PWT25_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1021074.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            36415..36903
                     /locus_tag="PWT25_000971"
     CDS             36415..36903
                     /locus_tag="PWT25_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1021075.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            36915..37409
                     /locus_tag="PWT25_000972"
     CDS             36915..37409
                     /locus_tag="PWT25_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1021076.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            37430..38239
                     /locus_tag="PWT25_000973"
     CDS             37430..38239
                     /locus_tag="PWT25_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1021077.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            38236..39057
                     /locus_tag="PWT25_000974"
     CDS             38236..39057
                     /locus_tag="PWT25_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1021078.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            39108..41207
                     /locus_tag="PWT25_000975"
     CDS             39108..41207
                     /locus_tag="PWT25_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL1021079.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            41204..42322
                     /locus_tag="PWT25_000976"
     CDS             41204..42322
                     /locus_tag="PWT25_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL1021080.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            42361..43239
                     /locus_tag="PWT25_000977"
     CDS             42361..43239
                     /locus_tag="PWT25_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1021081.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            43430..43582
                     /locus_tag="PWT25_000978"
     CDS             43430..43582
                     /locus_tag="PWT25_000978"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL1021082.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        14067 a         7573 c         9098 g        12869 t
ORIGIN      
        1 tggaggaaaa tatatggaaa attatcttag ccttttaggc gtcttgattg ttattctcgg
       61 gtttgcattt aaattagact cgatattgat cgtaatggtt gctttagttg ttacagcttt
      121 gactggcggt cttggcattc aaggaatgtt agaactttta ggtacaagtt ttgtaaacaa
      181 tcgcgggatg gcgattttta tcatcattat gttggcaaca ggaacgcttg aaagaaatgg
      241 tctgaaagaa tcagcggctt ccttgatcaa gcgattcaag aaagtatctg ctggtatgat
      301 tgtagatatt tatgggatct ttcgaatgat ctttgcggct tttaacgtga gttttggtgg
      361 agttgctgga tttgttcgtc caatcatttt accgatggca ttggggacca tcgaatcaaa
      421 agggttagaa gtccatccta agcatgaaga agaactaaaa ggtatggctt cagctatgga
      481 aaatgtctgc tggttctttg gccaagtact attcatcgga ggagcagggg cgttgctagt
      541 ccaatcaaca ctgaaagatc taggatatga cgtaacattg ggacagcttg ctttagtgga
      601 agttccagtg gctatcacgg ctttagttgt ggcaagtgtc tacttctatc ttaaagaaaa
      661 gaaattagcg aagaaatatt atccaggaaa ggggcaataa gatggacttt tttacaaatc
      721 ctgatatttt attaggcgat aaattactgg agatccttta tatcgtgatg ggactcgtat
      781 tgatttacac tggagtaaga aatttaacag ataaaaccaa tcctcatcgt tatgggtcag
      841 cctttttctg gacggtctta ggggtagtta ttgcaggagg aagatggctt ccggcaatcg
      901 tgaacggtgg cttgatcttt gcgatgacca taccagcaat tgctaagaaa gtcagcaaag
      961 gagaaagccg gcttccatca aaagaatata tggaaaaaat gtcagacaaa ctaggaatga
     1021 aaatttttat tccagctttg agtatcggtg tctttgcgat cttgtttgct ctatttacca
     1081 atttaggtgc tttagtcggc gtgggcgtcg gtgtttttgc agccatgcta atcttgatgt
     1141 tcttttctcg agataatcga ccaagtacat ttttagatga tgcagcagat atgctgggca
     1201 cagtagggcc gttaagcatg ttgccaatgc ttttagcttc tctaggtgcg gtatttacaa
     1261 gcgctggagt tgggacagtg atttccaatg cagtaggtac gatcgttcca gaaggaaatg
     1321 tgaacatcgg catcatcatt tacgctcttg gcatggtgat atttacggta atcatgggga
     1381 atgcatttgc ggccatcacc gtgatgacgg ttgggatcgg tgcaccattc gtatttagtc
     1441 atggagcgaa tccagcgtta ataggaatgg ttgcattgac ttgcggtttt tgtgggaccc
     1501 tgctgacacc gatggctgct aacttcaata tcgtgcctgt agcgatgctg gagatgaaag
     1561 acaaatacgg agtgatcaaa aaccagttgt ttattgcgtt atttatgcta gttttccaaa
     1621 ttgcttatat gattctattt aaataataga agagaggggt tgtgacagaa taattgtcgc
     1681 aacctcttct aacattgtag tggaacacta tttttttata agaatatatt aagtttgatc
     1741 tgttatgtta tttaaactat tgtttccttt atttacgctg ttcaaggaaa atggtatgat
     1801 aaaagataac catatgttaa atcaatcgga cggaggaaca acagatgaaa gtactagtaa
     1861 caggttttga tccatttggt ggggacaaag tgaatccagc ctatgaagca gtaaaaaaaa
     1921 tgccagatga aatcagcggt gctgaaatca tcaaagtgga agtaccgacc gtattcgaaa
     1981 aaagcagtca agtagtcaaa gaagccatcc aacagcacca accagatgtc gtgatctgtg
     2041 tcggacaagc aggtgggcgg tcagcagtct cgttcgaacg tgtcgcaatc aatctagcag
     2101 aagcaagaat ccctgataat gaaggaaatc agccattcga tacggcttta gaagaaaacg
     2161 gtccagccgc ttactttacc tcattgccaa tcaaagcaat gacaaaaaat gtacaagatc
     2221 atggtctacc agcgtacatc tcttatacag caggaacatt tgtctgcaat gatatcatgt
     2281 atcgtttgct tcatttgatc gaaacacaat tcccggcaat tcgaggtggg ttcatccacg
     2341 tgccattctc acccgatcaa gtgatcgatc gtccagtggg tacgccttct atgtctttag
     2401 aagacattgc ttcttctttg acttatgcag ttgaagcagc agtagaaaat aaagaagata
     2461 tttccggaaa tgccggaaca actcactaat ttagcaagaa acactggctg gttgctaacg
     2521 ttatgttagt agccagcttt ttgtaattag aaaggaaaga aacagccgtg gaagaatggc
     2581 aatccgtctt tgaagaatgg tttccaaaag aaatcagcaa aagctatccc atcaaaattt
     2641 ctaaacaata cacaagcagt caacgttggg agatctatgc aaaattaacg aaaaaacagc
     2701 gggaactagt cgataagcac cgccgatatc taatcagctc tcgttttatg gaagaacatt
     2761 atttagctgc aacagattgg gtgttttctg acttcaagat caatccgttt tttcgaacaa
     2821 aacgaagcca gcaaaaattg tattgtgagt gtggtagaga attaaaagtg caatacatcg
     2881 ttaaatcgcc aaaaaccgga aagatcctaa aactaggcat caaccacttt gccgatcacc
     2941 tgcatgtctc tccgacagtg gcagcttcga tccatcaggg aatgacaaaa gttgatctgg
     3001 ccttagacga attattatgg cttaagcaga agaatatcga ttttcctgaa ggactatggc
     3061 aaaaatactg ttttgtcttg taccaaaatc gtcggatgaa acagccatat ttaccggata
     3121 taaaattagc tcagcggctc gcagaatttc gccaagtaga aatgccgatt tacatagcgg
     3181 attatcaagc attggaaaat gaaatcaaga aaataagtga acacatcaat gggcagtcaa
     3241 aaaagcgaca gataaaaaaa gaactatttg acgactttgc agaagaatta gtaaaggatg
     3301 tagaagaatt cttgatcaac tatcgcgcat ttttacgtaa agattggcaa tccatcgtat
     3361 atgaagaagt gccagtacat cctaatgctt attttgaaac atttatttcc gtgttgagaa
     3421 agacaaaacg tcaacgaaca ccagaagtca ctgctcagat ggaatatttt gcgaaaaacc
     3481 aacgattcat ccaaccaaaa atctatctat ttatttggaa gcaatattgt cgctatggtt
     3541 ttacagaagg gttcttcgac agcattcctc ggatagtcag aaatggtttt ttaaaagtat
     3601 tgcgtaaaga acgagaagct atacaatctg ctgataagaa agaccgtaca gtctcaaaag
     3661 aaaaatggca actagtagtg aaagatatcc aatctggaaa tgtgcaagag acaatcgaca
     3721 aatggaaagg aaaacattat cgttttactg aagctcagaa acaagcgttg gaatattatc
     3781 agaagttaga agaatcactg cgtttcaatg atgaggcaag aaagtatctg aaggaattac
     3841 tataaaaaat agaggctggg acagaagcgt ttaactccaa gaaataagaa gaaattcacg
     3901 aaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgatt
     3961 ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct
     4021 cagttttttt atgcgtgttg ttgtgcgttg tagtattctt cttccatttt ttgcatacgt
     4081 tcacgttctt tttggttata ccaaggtgaa acggtaaatg caaggacatc attcagcaag
     4141 taaacagagc tgttcacgaa catcgccaaa cttgcagatc catgcgtaaa tgaaatatac
     4201 caaaggacca tttgagcaag ccctgaagct aaccaccagt aatattggtt gttgtaacgc
     4261 aagaaactga tgataccagc agtcaagcta atagaaaaag caatggcatc tacccacggc
     4321 cgaggatcat ctgtataacg accaatcagg aatcctgaaa caagatagac aaggaaagta
     4381 ctgatgattg cgataaacca gctttttcca gtgaattttc ggatttttga agccatatta
     4441 tgattccatt ctttacttaa caatacgggg atatccaatg tgatcatata agcaatctgt
     4501 tcaccgatac ttaaatagtt ctttgctgaa aacccaacga taataaaaca aatcgcagac
     4561 aaaataccta aaataccgtt gactgatttt gccgcattga tggataaaat acacaataca
     4621 ccaagcgtgg taccgataaa tgtaataacg gacagccaag tgatgggact attgacaaga
     4681 gtcatgacct ggcagccaag actgaaaaag aaaagataat aattttgttg cggccagcct
     4741 tttagctgat gagctaaaaa ctgaaaatat gacttcatgt atgaggactc cttaagacac
     4801 taaatgtagt gtgttctgta tttactaaca caatctattg tatgcttttc ttgaaaaaaa
     4861 actagtcttt caattataaa taatttgtgt tatgataatt ttctgtttgt tttcttgaaa
     4921 atataatttc ttactttcat ttattatatt gggaacagga agagtgtctt ggaggaccta
     4981 ctttctgttt tttttgcctt ttttcttata aaaaggaaaa acagctattt ttttcaatct
     5041 gacacattta caatggaact ggagattaat atagatccga gcttaaacga agaataggag
     5101 tgctcatatg aaccttacaa cgatccatca tatagccatc attgtttcag attatcagaa
     5161 atcacgtgat ttttatgtta ataaattagg atttgaagta atcagagaaa attaccgcaa
     5221 agaacgagat gactataaat tagatctgaa actaggaagt gctgaattgg agatttttgg
     5281 aattgctaat gcgccacaac gaccaaacta tccagaagct tgtggcttgc gtcatcttgc
     5341 tttcaaagta gacgatattg aagaaacaat tgcagaattg cagtccttag ggatcgaatc
     5401 agaaccgatt agagaagata cctttacagg aaaaaaaatg acgtttttct ttgatccaga
     5461 cggattgcca ttagaactgc acgaataaag ataacaaaat tgtcactttt ttttcgtttc
     5521 attcgatttt tcattcgttc atttcttgat attctttaaa aaaaagaaac aggagaatga
     5581 agaatgaaaa aaatgatgtt tggtgttgct gccctcatcg taatagggat taccggtatg
     5641 acgggactgc tcaatgccgc ttttcacatg gatgatatta gtgaccagga atatcggatc
     5701 tcattgaaaa aagcagcagc aatcgtaaaa aacgaaacaa aagaacataa catatctgct
     5761 attcattttg cagttgaaaa agatttggag aatcctacga atgcaacata tgaataccag
     5821 tttttttgtg aaggacaagt catagcagtc aatccatcca ctgggaaaac aaccgtacga
     5881 aatgacacgg aaaaggccgc agaacatctt tttgatatag aaaaaacgga agagttcaaa
     5941 caaccgcaag cagcgatgaa ggaagcagtc agctactacg ggaaaaaagc aaaagtgaaa
     6001 gagtggacac ttctagtcaa agatgaacgt ccttattata aagtccgatt aatagatgga
     6061 gaacaggacc gtcaggtatg gatatctgca tagaagaagg atattgcttc tcttttttac
     6121 aatataagag taagctaaga gagtaaagat gaaaggaagt tgagataaca tggaaaaaat
     6181 gaaatgccct aattgcggaa aaaaatttgc atacgaagaa gtcaataatg tagtcgaaca
     6241 taatgataaa gaaatgccag tcgtctgccc ttattgccga actgaagcgg caagaatcgt
     6301 tactcatggt tatttcatta ctgaaaagat tgaagatttt ttaaaataac gattttgcga
     6361 tttctttttc tgcatgttag aatggagaaa aggagaggaa actatgattc gttttgcgag
     6421 aaaagaagat gctccagaaa tcgccccatt gatcttagtg attctaaaag atatggaact
     6481 gccatttttg aagcagacag gggaacaaaa gactttagag attttagaag aagcaatcac
     6541 tgatccaaat tatcgttaca gttattcacg aggtatcgta gaagaaatag acggtaaagt
     6601 tgcaggagtt gcttacggtt atacagatga agaagaatca atcatcgatc agccactggt
     6661 acctatctta gagaaatatc agctagatgc gtctacacgt ttgttcattg ataaagaaac
     6721 gtttcccaac gaatggtatt tggactctat cagtgtatca gaagatttta gaggacaagg
     6781 catcggttcg cgattattag aagcattacc taaattggcg aagaaagcta atcgctccgt
     6841 catcggtcta agtgtggatg aaaaaaatcc aaaggctaaa aaattatacg aacgccatga
     6901 cttcaaagtt gtcggtcagc ggatgatcag tggtcatctt tatgatcata tgcaaaagaa
     6961 tatctaaaaa gatgtacctg aaatgcgtat ttccgcattt caggtacatc tttttaactt
     7021 aagttaagtc ggtaagaaac aggcttatcg cgacttttgt ctttacggta gagccagtaa
     7081 tctaaaaccg cagcaataca aatacaaact ggagcatctt catcatttga gacatccaat
     7141 acatagtaat ctcctgaaaa aagtgttgct ttatccattt tcatgatttg ctgattgaaa
     7201 tgatggattt gatattgatg attatagata tcaccttgga ctgtccagtg aagtctacga
     7261 atataataaa agtcaccggg ccaattgaat attttctgca tggttcccac cttttcgaac
     7321 tctttataca gctcaaaacg cgtaccgaat gtcaggctta tctgcttgat gtgagcaacg
     7381 agatcgccat tcatggcata aagagacaat gcatcgccct ttgttcccca gcgaccaacc
     7441 atcaaaaaca gggaatgtcc tttttcgttt ttgacaattg tccgagttgc acgtccaagc
     7501 tgcttttcct gaatgaaaaa ttctgacatc tatatccctc ttttctagat aaaactacaa
     7561 gtcttctgca atcgcgcgca atatggcttt tcctacgcca gattcaaggt ttgtgtccgt
     7621 tgtatatttg gcataatctt ttacttcgat ttctgcattt cccattgcga aactgactcc
     7681 tgctacttgc agcatgctga catcattgaa gttatcgcca atcgtcatga cttcttctaa
     7741 agaaatccca cgctcgtgag cgacatgggc aacagcaatt cctttttgag cgtttttatg
     7801 gttcacttcg atattgtttt gaccagagga agtaacggct agatcgccta aaagagaaat
     7861 ctgctttcca actggaccta atacagctgg cccttctgta tgaaaacaaa taatttttaa
     7921 aacttccaga ttcttttctt ccaaaatagc acgaatactg tccacgtatg tgatgtgcaa
     7981 taactccagc ttagctgagg ccatggcaat cgccatttta tacgtaaggt gaggtaaatg
     8041 ggtagccacc atggaagcaa aactttcaat tcgtttcgct tgattttcag aataaagacc
     8101 atcgttggtt gctacttcat agtaaatacc gttagcatct agaatatcta aaacagagat
     8161 tgcttgatta tttggaatcg acacggtaaa caatgaattt ccttctttat caaatacctg
     8221 tgccccgttc aaagtgatca tcgcacaatc gattcctacc tcatctaaag ctgcgcgagc
     8281 ttcttgatag tttcgaccag tagcgaccat gaactcaaca ccggcatcat ttgcaaaacg
     8341 tatcgcctcg gcattttcag gagaaacact catatgggca tctaataatg tgccgtccat
     8401 atctgatgca attaatttga tcataatgat tatcgttcct ttctgcttct tgttcctagt
     8461 ttaccatatt aatcaagtgc tgagggtgaa gattgtccct tgtgattgaa gaaaatgtga
     8521 gttattcttt ttattaggag gtggagtttt tgaaagaaat cattcataac agttggcaag
     8581 aagttttgtc ctcagaattt tcaaaagatt attatctgca tctaagagaa tttttaaaaa
     8641 aagaatatgc gtcccaaaag atccatccag atatgtatca tatctatgaa gcattggaat
     8701 tgactcctta tgaagaagtt aaagtggtga ttttaggaca agatccttat catggagaaa
     8761 atcaagctca tggcttatct ttttctgtgc aacctggcgt aaagatcccg ccatcgttaa
     8821 gaaatatcta taaagagctt tatgatgatc ttggaatcgc acctgttcaa catggaaatc
     8881 tagtgtcatg ggctaaacag ggagtccttt tactcaacac cgttcttacc gttcgggaag
     8941 ggcaagcgta ttctcatcgc gggaaaggtt gggaaagatt aacagacacg atcattgaaa
     9001 aattaaatga acgggaaaaa ccaatcgtgt ttatcttgtg gggaaaaccg gcacaagaaa
     9061 aaatcaaaat gatcgataag agtcgccata tcatcataac atctccacat cccagtccgt
     9121 tatctgcaag tcgtggcttt tttggttcca aacccttctc gaaaacgaat gatgcgctcc
     9181 ttgcgttagg agaagaaccg attgattggc aactgcctga aacagtgtaa taaaacagcg
     9241 ctttcctgtt atagtgcagt tcgctttgta tgtgaaaaac ttaatttttc cagcattttt
     9301 gactgataca gataaaataa actgttcttt ttttctggaa tagtgtatga taatacatat
     9361 aaaaacgtct ggaggtcatc ttgtggaatt gtttgatagt ttaagattta aaattgttcg
     9421 ccgtgggatc aaaatcgttt tcccggaagc tactgatgct cgtattttag gcgcagctgc
     9481 ccggttgaaa gcagaagaac ttgtggaacc cattttagta gggaacgaag aagaaataag
     9541 aaatgcagcg catgccagag gaatcaaaac atctagcttt acgatcatca gccctgataa
     9601 ttacgataag tgggatgaaa tggtggatac ttttgttgaa cgtagaaatg gaaaggcatc
     9661 caaagaacaa gcagaaaaga ttttaaaaga tgtgaattac tttggtacga tgcttacgta
     9721 tatggggatt gcagacggaa tggtctctgg agcgatccat tcgactggcg acactgttcg
     9781 cccagcactt cagatcatca aaacaaagcc tggcatcagt cggacaagcg gagcgatgat
     9841 catggtacgt ggaagagatc aagaaaaata tgtcttttcg gattgtgcga tcaacgtgaa
     9901 tccaactgcg caagaattgg ctgaaatcgc cgtagattct gcaaaaacag cagaattgtt
     9961 cgatatcgaa ccaaaagtag caatgatgag cttctcgaca aaaggctcag cgaaatcgcc
    10021 agaagtagac aaagtagttg aagcaactaa aatcgctaaa aaactagcgc cacaatacat
    10081 gatcgacggc gaattgcaat ttgatgcttc ttatgttcct tcagttgcac aactgaaagc
    10141 gccagattca gaagtagctg gtcaagcaac agtctttgta tttccagaat tgcagtcagg
    10201 aaatatcggc tataaaattg ctcagcgttt cggtaacttt gaagcaatcg gaccaattct
    10261 acaaggcttg aacaaaccgg tttctgattt gtctcgtgga gcaaatgaag aagatgtata
    10321 taaattatcg attatcacag cagcacaaac attgattgat taacaaatac acgcgaacta
    10381 gccaaaaaca gcagctgctg ttcttgacta gttcattttt tctttaaaaa aacttcatta
    10441 cgcgatatac tggcaaaaag aaagaagagc gggagaagat gaaaaatgat cgaactatca
    10501 ggattaacaa tgacagaaca attcgcagag gcaattggtc attccgcaat gccaggtgac
    10561 aatctgatat tgactggtga tctaggtgcg ggtaaaacaa cgttgacgaa aggaattgcc
    10621 caaggattag ggataacgca aatgatcaaa agtccgacat acacgattat tagagaatat
    10681 agtcaaggtc ggattccgtt gtatcatatg gatatttatc gggtagcagc tagcggagca
    10741 gatttagggt tagatgaata ttttgaaggt gatgggcttt ccgttgttga atggggaaat
    10801 ctattagaag aagcactgcc ggaggattat ttggaactga ttttagaaaa aagtgataca
    10861 gatttagaaa agcggtatgt gaaactaatc tcatacgggg agcaaagtga aaccttcaaa
    10921 cagcgaattc tagaaaaatg ggctgaaaat catgagtgaa gaagttggga tcatcttgcg
    10981 ggaagcagtg ccaggagatg caaaagacat tcttttgatg atgggacaag ttaataagga
    11041 gacagaattt ttagttttgg atgaagcaga actcctgtta ccaccagaaa ccttagaaga
    11101 agagttggat tatatatacg aaagcaataa taatttattg ctgcttgcaa tctatgaagg
    11161 gacgattatt ggaacagcat ctgtcaaggc agacagtcaa tttcgtttgt ctcacgtggg
    11221 agaagtcggt atcagtattc tgcaggaata ttggggaatg ggactaggca ctcttatgct
    11281 agaagaaatc atcagttggg cgaaggaaat gggtgtactt tttcgattgg agcttgacgt
    11341 tcaagttcgt aatgaaagag ccgtacacct ttaccgaaaa atgggcttcc aaatcgaagc
    11401 ggtcatgcct agaggggcaa gaacagatct aggtgaattt ttagatgtat ataaaatgag
    11461 ttatttgatt gaatagaaga aataggaaaa gagtgagaaa ctgtcatcct tttgagaagg
    11521 cagaaaaaca cacgaaggga ctgtgacaaa agtcgtgtcg cagtccattt tttttgaata
    11581 aacggtgttc aatcagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
    11641 tattttgccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttggt
    11701 aaacggtgtc caaaagcttg catctgcggt aataagtcca accaaatgaa aaatatgaga
    11761 agctattttt gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc
    11821 caataaacgg tgttctcctg ccagctcctc ggtcaccagt cacaatattt aataaacatg
    11881 agaagctatt ttttgaaatt gctccttgtg actcggagtg aaacggcagg ctcacgacct
    11941 ctttgtgtgt ttcataagta ttcttttact ttatagtcac aagttttttc aatggatctg
    12001 tcccgaattt tttttcttga taaagcagga tttccgcaca tgcacgacta tcttccaatg
    12061 catcatggtg atgatctaat tggatgttca aattttcgca cacagtgttc aatcgatgat
    12121 ttggaaattc aggaaacagg cgacggctac ttttcactgt acataaagaa agataattag
    12181 gctgttcaag atgataataa tctaagcatc ccgctaagac ctttgtatca aaagcagcat
    12241 tatgtgcaac gactaaacga tttttttgaa aatatggttg gatagtttgc cagacttctg
    12301 ggaatttcgg cgcattgcgt acgtcttctt gatggattcc gtgaatttgg acatttcgcc
    12361 agaaaaaagg agtctcaggc tgtatcaatg tatagaattc atcaacgatc tggctatttt
    12421 ttaccatgac tagcgcaaga gaacaagcac tataaggctg atgattggca gtttcaaaat
    12481 ccatggcaac aaaattcatt tctgtaaaac ctccttggga aattactgta acatactttt
    12541 gaaaaaagca aataaattta acaaaaaaca tagaattttt tatgtaatat ttgttgagaa
    12601 aagctagttt ttgaactcat tccttgtgat ttggagttag atggggatca tgacttctcg
    12661 gtattaagtc aaatttatgc aaagttgtga ggaaccattt tcaaaaaaat ttcttacgac
    12721 tcgaaagtag gcgacttttt cacaacttct ccactaaacg gtgttcaatc agctgaccct
    12781 cggtattagc tcaaaaaata gcaaaatatg aggaactatt ttgccatttg tttctctaat
    12841 actcaggtca ggacgctgat ttcacaactt ctcaataaac ggtgttcaaa agcttgcatc
    12901 tgcggtaata agtccaacca aacgaaaaat atgaggaact atttttgttt ggctgttctt
    12961 attacttgat gcagtgcagc tttttcacaa cctcttaata aacggtgttc aaaagcttgc
    13021 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
    13081 cttattactt gatgcagtgc agctttttca caacctctta ataaacggtg ttcaaaagct
    13141 tgtatctgcg gtaataagcc caaccaaacg aaaaatatga ggaactattt ttgtttggct
    13201 gttcttatta cttgatgcag gacagctttt tcacaatctc tcgataaaca gtgttcaaaa
    13261 gccagtttct cggtattatg ccaaatttat gcaaaatgtg gaaagccatt ttcacaaaat
    13321 ttttcttaat actcgaaact aaacgacttt ttcacaacct ctttatttca aatctaattc
    13381 tactggacaa tgatcactgc ccataatgtc cgtatggatc tttgcatcag ctagtcctgg
    13441 ctctaaatct ttgctcgtca aaaagtaatc gatccgccaa ccagcattgt ttttccttga
    13501 attgaatcgg tagttccacc aagagtagac accttcctgg gttggataaa aataacggaa
    13561 tgtatcgata aacccgttat ccaacagtcg agaaagagcc gctctttctt ctggagtgaa
    13621 tccagcattt ttttgattcg ttttccagtt tttcaaatca atcttttgat gagcgacatt
    13681 tagatcccca cacacgatga ctggtttttg tttttttaga ttttccaaat aattataaaa
    13741 ggcttcttcc cattctaatc ggtaatctaa acgttttaat tcgctttgag aatttggcgt
    13801 gtaacaggtt acaagaaaga aatcggaata ttccaaagta atcagacggc cttctgtatc
    13861 atggatatcg atacccattc cgtaagtggc attcaatgct ggttttttag caaaaatcgc
    13921 tgtgccggaa tagccttttc gttccgcgta attccaatat tgataataac ctggaagatc
    13981 taaatcaatt tgtcctgctt ggagtttcgt ttcctgcaga cagaaaaaat cggcgtctaa
    14041 ctcatgaaaa acttctaaaa aatttttatt tacgatggca cgaagcccgt ttacgttcca
    14101 agagataaat ttcaataaaa gcacactcct tcgaattaat ttccttacct ttgcgatact
    14161 attgtacctc attttagtaa aaaatggtgt aagatgaaag taactaaatg atccaggagg
    14221 aaagtagatg aagattggtg taatcggtgt agggaatatc gcagaaaaag catatttacc
    14281 aacttatgcg aaaaaacaag gaacacttga tttttatttt gcaacaagaa ataaagaaac
    14341 aaaagaaaga attcaaaaag agtacgggtt ccaacatcta tatgaaacga tcgatgagct
    14401 tttagaagaa aaaatcgaag catgtatgat ccacgcagca acaaaagtac attatgaatt
    14461 agccaaaaaa tgtctagaaa atggtatcca tgtttatatt gacaaaccac tgagtgtcca
    14521 tctaaatgaa gttcgtgaat tgcaagaatt agctgataag aaccaaataa ttttgatgat
    14581 cggtttcaac cggcgctttg ctcctatggt agaagaattg aaacgtatcc cagataagcg
    14641 gttgatccac ctgcaaaaaa atcgaatagc ggcaaaagaa acgacagagt tcgtccttta
    14701 tgatttgttt ttacatctta ttgatacagc tgtgtattta ttagatgaac ccattttgag
    14761 aacaaaatac cagataagag aaacaaagga atttttggaa tatgccattt tacaattaga
    14821 aacacaaaat caaacggcta ttttaacgat ggatttagca agtggcgcta atatagaaaa
    14881 atatcaagta acaagtaaaa atggtactta tgaagtaaac gatctgaccc agcttgtcat
    14941 tcaagaggct gatggaaaaa aagtgaaaga atttggcgac tggacaaaca cgctagtcaa
    15001 acgcgggttt gagccaatgg tagaggcctt cttttctcag atccgtttag gtaaatcgga
    15061 aacagaattg cttaaacaaa aagaagttgt aagaagtcat gaattatgtg aggaagtgct
    15121 taaagatcac taccgtcatc aactttgaac aagactggca agattgtatt aagcgtgata
    15181 gaataatgag gacgtttgag aaaagaaagg aaaatcctca gtgaataaag aagaaatagt
    15241 tgaaagctta gccacgatta ctatattgtt tgatgaacca ttgatgaact atacgtttac
    15301 gaaaacaggt ggaccagctg atgtccttgc attcccgaaa aaacaagaag aagtaaagca
    15361 aatcatcgat tattgccgaa tccatgatat accttggatg gtattaggaa atgcaagcaa
    15421 tctgatcgtt caagatggcg ggattcgcgg tgtagtcatc atgctgactg aaatgaaaca
    15481 gattcatgtc aaaggaacga tggtcattgc ggaagcaggt gcctcactga ttgatacgac
    15541 ttatgcagct ttggcagaat cattgactgg atttgaattt gcttgcggca tcccgggaag
    15601 tgtcggtgga gcggtttata tgaatgcagg tgcgtatggc ggagagatca aagatgtttt
    15661 tgctgaagtg gatcttttgc ttgaagatgg aacattgaaa actttgacaa aagacgaaat
    15721 ggtattttct tatcgtcaca gtaaagtcca agaattacgt gctatcgtat tggaagctcg
    15781 attcagtttg caaacagggg attatgaagc aatcaaagca agaatggatg agttgaccga
    15841 attgcgtcaa tcaaaacagc cattagaata cccttcatgc ggaagtgtct ttaagcgccc
    15901 agtaggacac tatacaggtc agctgatcca acaagcagga ttgcaaggcc tgaaatgggg
    15961 cggtgcacaa gtctctgaaa aacatgctgg attcatcgtc aatatcgatc atgcgacagc
    16021 gacagattat attgaattga ttgcgcacat ccaagaagta atcaaagaaa aatttgatgt
    16081 gtctttagaa acagaagtgc ggatcattgg agaaaaacaa gcaatatcag aaacaagcag
    16141 aagtttagcc aattaataag taatggaact taaaataagt aaaagagcag gacggtggcc
    16201 gtcctgctct tttttgtggt tgtttacgca aatggcgcaa atcttgtaca ttttaacaca
    16261 ataaaacaaa aaatatagaa tatttcctca taaatttttc cattttttgc aatattctca
    16321 aaaaaaagat aaaaaatgag aataactgat gaaaaaacaa gcataatcca tcataaaatc
    16381 agtataggtg aaaagatgaa ctttcatctg catatcaagt aattggtatg aggcattttt
    16441 tgaggtgtag gatatgaagt tggattacct tatagaagag gaagatagag aaaaactgct
    16501 gctagctaga tatttagaat ttgagaaaaa gaattatgta gtgctgccta agttatatga
    16561 aacattaggt ttgtctaaat ttaaaacaag aaattatatc atagaattaa ataaagaatt
    16621 tactgaattc aaaacaaagc cagagatcca aatttcagaa gaagcagaga tccatgtcga
    16681 acgtctgaat ttgacggata tcaaaaattt aagaagaatc tactttcaac gatcaaaaat
    16741 tgctcgactg ttaacggaga tcgtggattc aaaagtaacg atcgaaaaat ttgcagagaa
    16801 tcatttttta agcatatcaa gagcctatgt aaaaagaaga gaattagtct cttttcttga
    16861 acagcatcaa ataaagttaa agaaaaacat attagttggg gaagaaataa acatacggaa
    16921 cgtattattt ggggtatatt tcggggtctt taatggtttt tctatgccat ttagagaaag
    16981 cgtcatgaat gaagcaaaga gtatcgtgca atattttgaa tatctgttca atttaaaact
    17041 gtctgcaaca aaacgaatca aattcaattt gctaaccact attagtttgt tgagggccta
    17101 tgggaatcaa ccaatcggaa aacgtttctt ttcagatgaa tactttaaga gctcagaagg
    17161 aatgaagatc atcaaggatt tatcacatat ttcttttctt catatcccta aagagcagat
    17221 cattgatgag atttcttata tcatgttgtt tttgatcacg gaagaagaat ttgaattaga
    17281 tggcgcattt ggacttgcgg aagctcattt tcatgatatc cacatgatta gtcaaaaaat
    17341 gtcagtggaa ttgattgatc aattaccatt ttcgattgaa atctctgaag aagaaagact
    17401 atatatacga aaagaacttc gtatgggtat gatgcatgtc aatcgaaaaa attggatatt
    17461 cgactttgag atgactagct ttacgtctaa aaagcagcta cagtttttct tcgagacgta
    17521 tccaatattc agtacagcaa tccaaaaggt catacaagag aataaacgct atttcttggg
    17581 aaaagatgaa cgtttcatga ttcgcttatt ttatgattat ctctttttat tggtcggtat
    17641 ttgtccggta tcatattttg aagcaccgat tcatgtctgt attgattttt cgcaaggatc
    17701 tgcttatacc gattacatta tttctcaagt gaaaggattt aaaaattaca atattgtgat
    17761 tgagagtcga atcacttctc gtacgaatat ttatttatct gattgtttac ttgaaaaatt
    17821 taataaagaa cagataattt ggaaaaatcc accgactcca gatgactggg aatattttgg
    17881 aaataccatc atcagagaaa aaaatagaca tttaactgaa aaaatcagct agtatttatg
    17941 gatggcagct ttgtctcaca tatttattag ctgatttatg tttttttcaa agaaaaggta
    18001 actagatata actagatata actagatata ttacttaaca aaattaaaaa gtacataagt
    18061 tattattccg attttcttga aacaataaaa aatcaaaaaa aatactctcc taatagtcca
    18121 tcttcttttg attttattta tataatgaaa tcatcttatg aaaattacta cttcatgttt
    18181 agacaagagt agggaggact aagaataaat gtgttcaaaa agacgttcaa aacaactaaa
    18241 taaacacgct tgtcgtttag ataaggaacg gtcgtgttct gaaaaacact acgttaagtt
    18301 tatgatgcgc acgatgctga tcagtttatc tgtaggtgtc tatatgcatg tacccgttca
    18361 ggcaacgaca caaaccatca aggtcgtacg tacctctgtt tctgagacca ctgattcccc
    18421 tatcactatt actcatactt tcactgatgc aaatggaaaa cagctacttc ctgcattgac
    18481 ttcctataat aaagtcagtt atcctccaac gataaaccac tactggttaa ttcctaacaa
    18541 aacattatgg acatcggaaa ccggaagcag tgtgccgctt tctatctttt ccgatatgac
    18601 tgatcctaca aaaggtgatc agttaaaaca gatcactgat attttgaatc atgctttgac
    18661 agatataaag gacggtggaa gcgcacaagt ttcttatgta tatgaaaaag attattcaca
    18721 gtttgaaaga aagaaaatcg atgttctggc agggacaaaa gtttcgatat ctgatctggt
    18781 agaaaggctg aagaacgcag atggaacaca aggagatatg tccaaagtaa tttcaaacaa
    18841 tgggaatatc gtggatacga ctaatacagg aacatctact atttccttgt cctattttga
    18901 tccaattgct ttaaaaacaa tgactgcatc tgctgtgatc catgttcgtg ctgatcaaac
    18961 agctatcaaa ggaaaagatc ttcacttaag aacaggagag agttacgccc ctaaagatct
    19021 tgttgaatat gtaacaaaaa gcgatggaac ttcaggaaat aaagaagaaa tcaaaatgaa
    19081 cgggacagta gatactacaa ctccgggaga atatcccgtt gtattaactt atatagactc
    19141 gaaaacatca aatgtggcag aaacaacagc tgtagtttat gtagaagcat ctgctgcttc
    19201 atcgactgtc atcactcatt ttatagatag tcaaacaaat aaagaacttc aagcagaaac
    19261 acatatgagt agtgtaaatc gtattcctga tttgcgccta cctgctgaat tttctgatcg
    19321 agtgccaaat atagatttat cgacttatga acaaaatggg aaagtcactt ctatttctga
    19381 ttggatgcaa gcaaacggat tagacagaaa aagtcactgg tcggagatcc ttgaagtact
    19441 tcactcagaa ctgatgaata ataggaagca atcaggaggg gaaattacct ttacttatgt
    19501 ttatatacca gacaatcgtt cattagaagg tcatgatctt attgttcatc caaacgaaaa
    19561 aataaacaaa gaagagttga tccaatcagc aacaaattcc ttaggtaaac ccgtaccgtt
    19621 aaaggatgta tcggttacaa tcaatgaagt accattggag gatggttatt tgaccaaaca
    19681 aacaggtgat tttacgatta tgtatagtta tcatgacccg tattcactcc aagaactcca
    19741 tgctcagtca ctattacatg ttttaccatt aaacaaacag atagatcaaa aagaacaaaa
    19801 aagacgagaa gaaagcttgt cagaaaaaaa acaacaaatg gctcatgcag tttctccaac
    19861 agggaaagta aaacaaacaa gaagcctaga gttagcaaga atatctagaa aaatctcaga
    19921 atctaataaa acgataaggc aagaagctca tttggcccca caagtttctg gattggaaaa
    19981 tgaaatttta gagcctaaaa taaaacaaca atcagaaaaa gaatttgttg ctaaacagtc
    20041 ggaagatcag aaggaatcag atgaccagaa ggaaacgaag aaaaaacgct tgtcaactat
    20101 tgattccacc gatgagcatc tgcagccgca agtaccactt cctagtccgg gaacacccgc
    20161 acctggtgga ggtattcctt cttctgcttt aggagatttc atgcgaggta tggcaggaac
    20221 atgggtatac gctaatcgaa atgaatttga cgcagatgac tggttctgat ttatatctct
    20281 atctgttata gtcaaataag tagctgagtc atatgttatt tggcataatg gagacgagga
    20341 gggatagaaa aatgttagat aaaacgttac cttacgcaga aatctggatg acaagaccac
    20401 ttcaacaaaa tgttccaatc acgcctttag cggaaggttt tgaatttcga ttttatcaaa
    20461 aagaagatga atctgcttgg gcggagattg agacttccgt tggggaattt caaacgacag
    20521 atgaagcact tgagtatttt caacaaaact ttgctcctta tcctgaagaa cttgaaagaa
    20581 ggatgttttt tgtagagaca aaaaaaggag aaaaagtagc aacctgtacc gcttggcgaa
    20641 aaaaatcaag agagcaagtg tatccggttt ttcattggtt agcagtgaaa ccagattacc
    20701 aaggaaaagg gctcgcaaaa gcattgactg ccagagtctt gcaagaattt cctaccctcc
    20761 atacggacgg accaatctat cttcacacac agacatggag ccatcaggcg attgctcttt
    20821 ataaaaaaat gggttttact tttattgctg aaaatctaga cggcacacct aatccagatt
    20881 atgaaaaagt tatgcaggtt ttaagtaaat tagataaata acgaaatggt gaaataagtc
    20941 ctcagtattg ggataaaaaa cgaaatagtc attcctcata tcttactttt ttgagttaat
    21001 actatcggat aactaatggc atgccataca ttcaaaaaag aaggggcagt gacaaaagta
    21061 ttgtcactgc cccttctctt gttttttagc atgttttatc gtttgatttt tttaggaata
    21121 aaattcttga tgaacgtaaa tttttggtaa tcgtttcgac tatagaggta accaaataca
    21181 ctgaaaacaa ctgctccaat aaaattaaca aataaatcct tcatagtatc catgattccc
    21241 acgtctaagt aaccattttg gatgattgtt ttatctgttt taccatctgc attttgactt
    21301 tcaatgattg tccgagtgat atctggaaca tgatagacag tgtttccgtc tttacttaac
    21361 tccacactat tgatttgctg gatgatcgta tctttttgca tatcgaattg cagtaattta
    21421 tctgctgaaa attccacaaa ctcccacata acaccaactg tcatcgaaaa acagaaggaa
    21481 acaactgcta gtaataaagg agtcaagttc atggtttttg tatttttatt gagcaaataa
    21541 acaagggaaa agccgacacc cccagctaaa aatccgctga atgcatgtag aaaagtatcc
    21601 cacattgaaa aatagccata aaaatcactt aactctccta aaactgttga actaaaaata
    21661 aaagataaca aaatgatttc tagcatattt ggaatctgta tctttaatat cttttccaga
    21721 aaataaggga tttcaaataa aattaatgtc agtaataaaa gcattgtatt tccccagtgt
    21781 tggttccaga cctgatccac tagcataata acgacgatga tacgtaataa aatatagatt
    21841 ccgtttgtga ttctacctgc agtttttgta ctgttcgtaa aaaaggtttt caaatgattc
    21901 ataaaataac tccttcatgt atatccttaa ttatactaag aagcgtgtgt ttaccaaagc
    21961 tatatgactt aaagaaaaac agagttgata ttgtgaaaaa aatcacttaa tatagtataa
    22021 ggagaaaagg gggatacaaa tgaaaaaatt agttgctggt ttattaacag ccgttgcact
    22081 tcttgctatg agcgcatgcg gcgcagatca gaaaaataca gcgcagacaa gttctagtac
    22141 gaaggaatct gccgaagtat catcaggcgc ttctgaacaa gtatatacag atccaaaaga
    22201 aatgaaagaa tcttatgatg tcgtgattat tggagctggt ggtgcaggga tggcagcagc
    22261 tatcgaagca aaagatgctg ggttgaatcc agtaattttg gaaaagatgc ctgttgccgg
    22321 aggaaacaca tctaaatcat caagcggtat gaatgcttca gaaacaaaat ttcaaaaaga
    22381 agaaggaatc aaagactcga atgatgcctt ctatgaagaa acgctaaaag gtggtggagg
    22441 aacgaatgat aaagaaatgc ttcgatactt tgtcgatcac tctgctgatg cggtcgattg
    22501 gctagatcaa aacggtatca aactggacaa cttaacgatc actggaggga tgagtgaaaa
    22561 gaggacacat cgtccgtctg atggctcggc tattggtgga tacttagtgg aggggttatt
    22621 gaataacgtc catgagcggg aaatccctat ttttgtcaac gcagatgtga cagacattaa
    22681 gaaaaatggg gaaacggtca atgaagtgac tgtacatgtt caaggagaag aaccaaaaga
    22741 ggtcaccgga aaagcaatcg tagttaccac aggcggtttt ggtgccagca aagaatttat
    22801 tgaaaaatat cgtcccgatt tagctgatta tattacgaca aatcaagaag gatcaactgg
    22861 agatggtatc accatgattg aaaaattagg tggacaaacc gttgatatgg ataaaataca
    22921 aattcatcca actgtgcagc aggatgaggg ggtcttgatt ggtgaggttg ttcgcggaga
    22981 aggagcaatc ttggtcgatc aagaggggaa aagatttgtc aatgaaatgg atacaaggga
    23041 taaagtatct gcagcaatta cagcattgcc tgaaaaatca gcttatttga tttttgacca
    23101 aggcgtgcgt gatcgtgcca cagctattga attttatgac caaaaaggat atgtcacaac
    23161 tggaaagaca atggaagaat tagcagaaaa aatcgattta ccaaaagaaa cactagcaaa
    23221 aacagttgat ggatggaata cggatgttgc ggagaaaaaa gatgctcaat tcaaccgtgc
    23281 cacagccatg gaacatgact tatcgtctcc gaattattat gcgatcaaaa tcgcaccagg
    23341 aatccattac acgatgggcg gtgtgaaaat caatacgaac actgaagtat tagataaaga
    23401 aaaccagcca attagggggt tatatgctgc tggtgaagtc acaggtggct tacatggaaa
    23461 caaccggatc ggtggaaatt cagttgcgga gatcattatt tttgggcgac aagcgggcat
    23521 acaagcagct cgatttgctt cggaaaatga atgaaataga acgtaaataa aaagaggttg
    23581 tgaaatcagc gttttgagct aataccgagg gtcagctgat tgaacactgt ttagtgaaga
    23641 ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt
    23701 atgtttttaa cttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    23761 ggaaaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
    23821 aacggcggaa gcagaagaaa ttccttcgga aataagctga aattcacaaa aatttgaaaa
    23881 gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt
    23941 tttgatcagc atgattttat tcagttatct tcaaaatgat ttttccgatg tttttgtttg
    24001 cttccatata ttcatgcgct tgtttgactg cgctgaattg gaatgtacgg tcaatgattg
    24061 gccgaatctt gccattttct agaaaaggcc cagctttttg cataaaagtt tgtgcgagct
    24121 ctgctttata ctgatcactt ctaggtgtca ataatgtacc aagcagttgg acacgtttcc
    24181 caatcaatac accaaggtct atctcaggca cgatggtccc acctagcata ccaatcaaga
    24241 cccaacgtcc atcaacggct atagaagcta aattcttttc ccagtaagaa gcgccgatga
    24301 aatccaaaat aacatcgact ccccgacctt cagtgatttt tcgtacctct tcttgaaagt
    24361 cttgtttttt atagttgatc aattcatctg caccaagttc tttacaaaag gcgagttttt
    24421 cttctgatcc tgcagtaacg atgattttcg cgtgagaaag ctgtctagct aattggatcg
    24481 ctgctgttcc cacaccactt gctcctgcat ggatcaagac agtttcgcct tcttgtaatt
    24541 ttccaagcca gtaaagtgtt tgataagcag tcaaaaaaac ttctgagatt ccagcagcag
    24601 aaacatagtc tagcgtatca gggagaagga ttgcacgatc ggcaggcatc acagcatatt
    24661 cggcataacc acctagattg actaatccgt atacttttgt cccaggagta aaagtggaga
    24721 tatcactttt attctcaact acctctccag ctacttcgac tcccaaaata gggtagggag
    24781 gtttcaaaga gactgattct cttttcataa tatctgttcg attgacagct gctgtatgga
    24841 ctttgatcag taattcgcct tgttttggtt taggaacagg cacttcaatt tgttctaata
    24901 catctacaga accaggatgc ttgattgaaa tggcacgcat ggatctattc ctcctttgcc
    24961 cattcttgga atgctgtatt caactctttg ctaccttcag aaagtactag agtcgtacga
    25021 gaaatatccg tttcttccac tagagcggca agtacagcag caactgtaga gcgagtgatc
    25081 gtttttgatg ccttctcatt aggcttagtt aattgaatgg aagtaacttc ttcatcgtta
    25141 gtcaatgtga ctggttgaag aatgacaaaa tctaagttag ttcgtttcag ccattcatct
    25201 gcataatgtt tagtaatata ataatctgtc atggactcag gccattttgc tggttcatct
    25261 gcataaacag cactgaccat cagatagcga gagacatgcg cattttcagc tgcgatcact
    25321 gttttgattg cgccatccaa gtctacttgc agaagattct ttccttgaga gccggcagca
    25381 aaaatcaata catcgatgcc tttgaatgct tcggtcattt tttccacgga ccaagttaaa
    25441 tcaaaagaga catacgaaac attctctttt tttactaatg tttgggaagc aacatctctc
    25501 acccctgcaa aaatttcatg ggaacttgga gctaaatctt ttatcaggtg tcggccaatt
    25561 tgtccgtttg cacctacaac aaaaattttc atttggatca tcctttcgac gtcaaatctt
    25621 tccattcaat agtaacacca aaagagaaaa aaatcagaga tcggctacga tgactatcag
    25681 ttagtaagag aagggtgctt gaagtatctt aacaagacat tgcatactgc tgcagtcgca
    25741 agacctgcga cgattccttc aggtatacca cttgtaaaaa taaccgctag aatagctgaa
    25801 taaacagcat ttaaatttgt tccgatgact ttggcataat cttgctggaa taaaaagtag
    25861 atcatattca tcactaaaat cgtattggtc aatgaaccgg aaagtccagc taagaataaa
    25921 gaaacggaat ctgctttttg tttcattagt ttgcgcagac ctttataaac gaaataagga
    25981 accaccccaa tcaagatgcg aggaataaac gcgatcagca aggctttcca gcttccatgt
    26041 tctgttccga tgactgggat aaacggagaa aagacaaaag acaaaggtgt ttggataact
    26101 gtgctacgga tcatgctgat cagtccaaat gtaccgccaa gaaaagcacc tagtcttggt
    26161 cctaaaacga tcgaagcaat gattacagga atatgcatgg ttgttgcatt gattggaccg
    26221 atagggataa aacctaaagg cgtaacagct aataaaatca aaatagctaa aaacatcgca
    26281 gttaatgtaa agttttttgt atttttcata agtactcctt ttttctaagc atttctttca
    26341 ttaagagtca gcatgacctg ttcgatgatc gtctgaatat cagctaaggc accttttcca
    26401 aaatctccac aagctaaaag agattctctt ggttcaattt cctgatagcc gacagatctc
    26461 aatgtttcca gattacgctg agtgatcggg ttttgataca tattcgtgtt catggcaggg
    26521 gcaatcaatt ttggtgttcc cactggaagc gccattgcga ctgttgaaag aagatcgtct
    26581 gcaatgccgt ttgctagctt gccgataatg tttgctgtag caggcgcgat gagaaacaag
    26641 tcagcttgtt ttgctagttc gatatgattg attacctgcg gatttgattc ttgcatgacg
    26701 tctgtatgga ctgcgcgctt cgacagagat tgcaaggtta atggcgtgat aaaatgtgtg
    26761 ctgcttttag acataatcac atctacctgg gcacctaatt tgacgaattg acttgtaaga
    26821 tcagcgcttt tgtaagcaga aatacttcca gtaacgccta gtaagatctt tttcattttg
    26881 attcctccag tctaattttc tcagtaatca atgcagcaat ctcagattta ctttgagctt
    26941 cttccactgt tccatcttta gataagagat agccgtggtg tgtctcgcca tggatttccg
    27001 tcaaatcatt tgcgaaaata aaatcagttt tgttatttat cagactattt tttgcgactg
    27061 ccagaagaga ttcctttggt acatctacta atagtttgaa accaatgatt tttgcttttg
    27121 gctgccattc acgtagataa gaaatgattt ttggtgtttt cttcaaaacg agcacaagat
    27181 gctcggtgtt agaagagatc tttgaaacag tggtatctgt ctgttcattt tccgcaaacc
    27241 aattgtccag ttcttcttgc gttgaagaag tgggtaggtt ttttgccagc tgttcttctg
    27301 aaaaactaaa agcaggagta aaatcactga ctgccatact gtggataatc gcatcgtagt
    27361 gttctttttt cgttagttct tctaattgta aaaataagtc cagtgcagat tcgatcgtat
    27421 aaatcgttat attggagtgt cttttcggtt tcagtgcttg tttcgtagtc acgtaatcga
    27481 ttgtggtatc cggattggca gctaaataat cagctaatgc ttgccctaat cgtcctgtcg
    27541 aatgatttgt aatagcacgt acttgatcga ttttttcagt cgttcctcca gctgtgatta
    27601 gaatcttcat ttatggtcac ctacatttac tagtgttctt ccttggtgtg ttccagcaag
    27661 tagtttagat gctgtttcag gcaattgatc cagagtaatc tcttggacga ccagttcatc
    27721 tgcaatctgc aaatccgcca tctgttgcca taaagaaaga cgttgatcta ttggtacatt
    27781 gacagaatca attccaatca actggatgtt tcgcagaata aaaggcaaga cagtcgcgtt
    27841 tagtttgatt cccccagcat tgccacataa agctactgca ccgttataag agatcaatgg
    27901 aagaagactg cttagctgct cgccacctac tgtgtcgatg acatagtcga tttgctgttt
    27961 tcctaaagct tttgttgtct ctggcagaaa ttcatcaggt cgtacgactt gcaaagcacc
    28021 tagacttttt agccatgtga cagactcttc ttttctggaa aaggcaatga ttgactggaa
    28081 tcctagttta tgtaacaggg ctatcgctgt actgcctaca ccgccagaag caccagtaac
    28141 gacaactttt ttatcggagg tcataccttg acggattaaa gcgttcacac acaacatagc
    28201 agtaaaccct gctgtaccaa ggatcatcgc ttgacgcagc gtcatatttt ttggtagagg
    28261 aactaaccag tctcctggta ctttttgata ttggctataa ccgcctggat gggtgacgcc
    28321 aagcccaaaa ccagtagcaa gtacaagatc ttcttttgaa aaacgtggat tgttactttg
    28381 gacgacagtt ccggataaat caatccctgg agtcatcgga tatgttcgta tcacctgacc
    28441 agactcgctc attgccaatg catctttata attgacatca gaataggcaa tttttaccaa
    28501 gacttcattt tcagccaact cagtaaatgg cagatcactg attgctgtat ggaaaacagg
    28561 ttcctttatt acctgaaaag atcgaaaagc gttcattcgt gagacactcc ttttaaaatt
    28621 ttcttttatc ataccataaa gcctactctt ttattcttgt tcttccgtaa atttagacaa
    28681 aaattaggtg aaaaataaag aatcaaacaa ctagaattta ttccaaaaga taaagaaata
    28741 ttcagcaaaa aaaattttac aaagggttgc ctttttcaat aaatttcgta tactaatacg
    28801 agtggtagta aacaggaagt ttactattag aaaacttcga ttcggaggaa attatggaaa
    28861 ttggtgagaa gttaagaaat ttgcgcatcc aaaagaattt gacacaagaa gaactaggcg
    28921 aacgcacaga tttgtcaaaa gggtatattt cccagttgga acgagattta agttcaccat
    28981 caatggaaac attcttttct attcttgaag tcttaggtgt gactccagaa gaattttttc
    29041 atcaagaagc agcgaatctg caagtggttt attcaaaaga agatcatacg gtttatttcg
    29101 atgaagaaaa cgggtatgaa ttggaatggc tcgtggctga ttcgaatgaa aaagaaatgg
    29161 agcctgtctt gctgactttt gaaggtgcag gagaatataa gacgtttgaa ccttcattat
    29221 ctgaaacgtt tatttttgta ttagaagggg aattagagtt aagcttagga gagacggtct
    29281 atgtggcaaa agcaggacag tcgatctatt atcaagcgac aaaacaacat caattaagga
    29341 atcatctcaa aaaacgaaca aaagtattga ttgttgcaac gcaatcatat ttgtaatata
    29401 gggggtaaaa acgtggggaa accaatcatt tcatttgatc aagtagtgaa acgctatgat
    29461 gatgaaacag tattaaaaaa agtaagtttt gagatcgaac aaggaaaatt ttatacactg
    29521 cttggtcctt ccggctgtgg taaaacaacg atcctacgta ttatagctgg attcaccgaa
    29581 gctacagaag gggacatcta ttttgaaggg aaacggttga atgatttgcc agctaataag
    29641 cgacaagtaa atacagtctt ccaagattat gcactttttc ctcatatgaa tgtttttgat
    29701 aacgttgctt ttggattgaa gatcaagaaa atgtccaaat cagatatcga aaagaaggta
    29761 aaagatgctt tgcgtatggt ccagcttcct ggatacgaaa aacgggagat tagtgaaatg
    29821 tctggtggtc aaagacagcg tgtagcgatt gcacgagcaa ttgtaaatga accgaaagta
    29881 ttgctattag atgaaccgtt atcggcgctt gatttgaaac tacgtacaga tatgcagtat
    29941 gaactgcggg atctgcaaca acgattagga atcacgttta tttttgttac tcatgaccaa
    30001 gaagaagcgt tggcaatgag tgatgagatt tttgtcatga ataaagggaa aatcgtccaa
    30061 agtggtacac ccgtagatat ttatgatgag ccaatcaatc atttcgttgc tgattttgtt
    30121 ggggagagca atatcgtgga cggtgtaatg atcgaagata atctagttga gtttgttggt
    30181 aagcaatttg aatgtgtcga tggcgggatg cgtctaaatg aaccagttga agttgtttta
    30241 cgcccagagg atttgacgat cacgacacca gataaaggaa aactagtcgt aacggtcgat
    30301 actcagctgt ttcgtggtgt ccattatgaa atcatctgtt atgatgaaca gcaaaatgaa
    30361 tggatggtcc attcaacgaa aaaagctaaa gaaggaagca aagtaggatt ggcctttgag
    30421 ccagaagata tccatgtcat gcgtttcaac gagtctgaag aagaattcga tgcacgtctt
    30481 gacagctacg aagaataggg gggacaagaa tgaaaacaat gcgcaggatc tattccgtcc
    30541 cttatatttt gtggctcggt ttattcgtga tcgcgcctgt attaatgatc atttatcaat
    30601 ctttttttga tatgaatggg caattcacgc tgaataatta tctatcttat tttacgtcgg
    30661 ggacatcgct ttctatgacg atcaattctg tatggtatgc atttttgatc acgtttttca
    30721 cgctgatcat cagttacccg actgcttatt ttttaacaaa actgaagcac aagcaattat
    30781 ggctgatgct gattattttg cctacatggg tgaatctgct gcttaaagct tacgcattta
    30841 ttgggatttt cagtgtgcac ggcagcgtga atcatttctt tgattttatc gggatcgggc
    30901 cgaaacagat attattcaca gacttcagtt ttctgttcgt ggcaacgtat atcgagattc
    30961 catttatgat tatgccaatt ttcaatgcat tggaagagat gaatccttct ttgatcagtg
    31021 ctagtagaga tttaggcgcg aatagctttg agaccttccg acgagtgatt tttccgttat
    31081 cattaaatgg tgtgaaaagt ggtgtccaag cagtatttat tccttctctt tctttattca
    31141 tgctgactcg tctgattggc ggaaatcggg tgatcacttt aggaacggct atcgaacagc
    31201 atttcttggt tacacaaaac tgggggatgg gttctacaat cggtgtgatt ttaatcattg
    31261 ctatgttcat cgtgatgctc ctgacaggcg agaagaaaaa aggaggtcgc tcataaatga
    31321 aaaaatttaa atggtcgtat ctttatttag ggatcgtctt tttattctta tatgcaccga
    31381 tcttctatct gattttttat tccttcaatg aaggaggaac gatgaacagc tttacaggat
    31441 ttacttggga aaattatgaa gctgtttttg aagatacacg tttgatcatt atcgtattga
    31501 atacgttcat gctggctttc ttatctgcat tgatcgccac gatcatcggg acatttgggg
    31561 ctatgggaat ctattacacg aaaaaacgac gtgcacggac agcgttgttg agcatgaata
    31621 acatcttatt agtctctcct gatgttatta tcggtgctag tttcttgatt ttctttacac
    31681 tactaggatt cggattaggt tttcagagtg tattgctttc ccatattgct ttcagcattc
    31741 caatcgtcgt cttgatggtc ttgccaaaat tgcaggaaat gaacgattca atggtcgatg
    31801 cggctagaga ccttggtgct aataatttcc aagtagtcaa aaatatcatt ttgccttatc
    31861 tttctccagg aatcatagct ggatatttta tggcatttac ctattctctt gatgattttg
    31921 cggttacatt tttcgtcaca ggaaatggct ttacgacgct ctcagtagag atctattctc
    31981 gtgcaagaca aggaatcagt ttagaaatca atgcgctaag tgcgttagtt ttcttgttct
    32041 ccatgatatt ggtcattgga tactacttta ttagtaaaga aaaccgtcca aaacggctga
    32101 agaaaaacag aagagtacgc gagggggtgg cacggctgcg atgaaaaaat tacaatctct
    32161 gtttttaggt attctcatca ttattgcggt gctgctgctt ggtgttcgtc aacttgaaaa
    32221 aacaagtggc atgagtggag caaaggtact taccatctat aactggggag attatatcga
    32281 tcctagtttg atcacgaaat ttgaaaaaga atatggatac aaagtgaatt atgaaacatt
    32341 tgattctaat gaagcgatgt tcacaaaaat ccagcaaggt gggacaaact acgacatcgc
    32401 tataccaagt gaatatatga tccaaaaaat gatcaaagaa aaactagtac ttccattaga
    32461 tcattcgaag atcaaaggat taaataatat tgatccacga tttttagact tggattttga
    32521 tccagataat cagtattcga ttccatattt ttggggaact ttagggatcg tatataacga
    32581 taaattcttt gatgcaaatc aaattaagca ttgggatgat ttgtggaagc cggaattaaa
    32641 agacagtttg atgctgattg acggtgcacg agaagttatg gggctttctt tgaatagttt
    32701 aggctattct ttgaatagta agaatatggc acagttgaca gaagcagcaa ataaattgaa
    32761 tcatttaaca ccaaatgtca aagcaatcgt ggcagatgag atcaaaatgt atatgatcaa
    32821 tgaagaagcc tccgtggcag tgacattttc tggcgaagct gccgatatga tgtgggaaaa
    32881 tgaacattta cattatgtga tcccaccaga aggatccaat ctttggtttg ataatatcgt
    32941 tatgccaaaa accgtgaaaa ataaagaagg tgcctatgat ttcatcaact ttatgctaga
    33001 accggaaaat gcggcacaaa atgcagagta tattggttat tctacaccaa atgaagaagc
    33061 aaaagcgtta ttgccaaaag acatttcgtc ggacgaacaa ttctatccat ctgatgacac
    33121 gatctcccat cttgaagtct acaaagatct aggatcgaaa tacctaggca tctataatga
    33181 cctattctta gaattcaaga tgtataggag ataatggcaa aactcagtaa gaatggggac
    33241 ttatcaacgt tctaaagagc taaaaatcgt cctttttcta tctgagtttt ccaatcttct
    33301 accaaaagcg gatgaaacgc cgcttttttt gtgttatttt attttttcta ccacgttttt
    33361 ctaccagaga aaagtggaca aaaccattgt aaaatagggc ccgagacggg ctagtaaatc
    33421 taagattgaa aaagttacag aaggataaag taattggaaa ctatgcaaat atacttaacc
    33481 cttctgtaat ctattaataa aactaatata tgaatccagt tcaatatttg tagctatgaa
    33541 aaaacataaa aatcatatta gataaaaact aaattggtag ataaattatt ttgtcagata
    33601 tctgaggcaa atattcgact atttgtgttt caaagtaaaa attggaatac aaatatagaa
    33661 tagacatatt gcagccgata gtttattatg cttttagaca cattaaactg gaggaaaaaa
    33721 tgaaaaagag cgctaaagat cgagtttttg atgagctgat acgactaaca gggaatatgt
    33781 cttctgtcac tgccgagaaa atttcgaaga atctaggtat ctctcgtcag aatgccagcc
    33841 attatttgac gagattagtg gaagataaaa aggtcgagaa attaccggga aagcctgttt
    33901 tgtggaagcc tttagatgaa tatgcggttg tagataatac ggagcaaata aacgaagcct
    33961 ttcattctgt agttggtcat gacggaagtc tgagagaggt cattcaaaaa tgtatagccg
    34021 ctgtcaaata tccgcctaac ggactcagtg tattaataaa tggagccacg ggtgttggaa
    34081 aaagtttttt agccacaaaa atttttgaat acgctatcca tgagcaaatt attgaaaaag
    34141 atgcaccatt tgctatctta aactgtgccg attatgcgga caatcccgaa ctgctttcgg
    34201 caacactgtt tggatacaag aagggggcct ttacgggggc tgaaaaggat accgatggtt
    34261 tattggcaac ggccaacaac ggctatcttt ttctcgatga ggtccatcgc ctatcgaaag
    34321 aaaatcaaga aaagctcttt ctatttattg atactggaaa ctatcgtcca gtcggtgaaa
    34381 atgtaaattg gcattctgct aaggttcgtt ttattttcgc cacgacggaa aaaggtgaaa
    34441 attatttatt agacaccttt gatcggcgta ttcaaattag tgttatgttg ccaacttttg
    34501 aagatcgccc cattcgagaa cgattagagc tgattcaatt attttttcaa aatgaagcgg
    34561 atgttttaca aaaagatatt attgtttcaa gagaagcctt gtctttaatg ttatctcatc
    34621 cctttagtgg gaatattggt aagctgaaga atatcattaa aatcagttgt gcggatgtgt
    34681 atagtcgaac acaagaagaa ccattaacga ttgggaaaaa tgagatcttg attcagttaa
    34741 atatgctgga atccgaagta gaaagcttgc cgattgctag tttattgata aagaaaaatc
    34801 aaaagataaa aacggatcga atcaaaccgt ttgattatga ttcgattttg gaaaaaatta
    34861 ctcagctgat aaatgaaggt aatttcaatg aaaatttttc ttcgataaag aatgaagtac
    34921 agcatttggt gcatcacatt gataaggatt cttttttatc aatgagtaga aaattgatga
    34981 ttcagatgtt tagaaaggtc tgggtatccg ttttaggaaa aacgtacgga ttgacgacga
    35041 gtggacctgt tgcagaaatt attagcagat cctatgtcat ttccccaagc tataagacag
    35101 acttaaccgg gataaatcgt acattagctg caaatctacc tcggacgact tacttatgta
    35161 atcagttctt ggagcattta ccaccgctct cagaaaagga cgagaacttt ctaagactgc
    35221 ttattgtcgt gactttatct gattatgtag atgaatccat cgagctgaag ggcctgttgc
    35281 ttgctcacgg agatagtacg gctagcagca ttcaagcagt agtgaatcaa ttatgcggca
    35341 attatgtttt tgaagcgttg gatatgccag tagagagtaa tgtgtcagag attgtcgaat
    35401 cgacaaggaa tttcgtgaaa cggcaagtga atacagatag tctagtgctg atcgtagata
    35461 tgggctcttt aaatcaaatt tacacacaga tcaagaacga tttaaaaggg gagttacttc
    35521 ttctgaataa tttgacgact tccattgcac ttgatattgg cttgaagatg acgatgaatg
    35581 cgccatttaa acagattgcg gaggatgcag cagacaagta ttcaatcaat gttcaatact
    35641 ttgaaggctt ttctcaaagt agcaacatta tcatttcctg tatgtcgggt ttggggattt
    35701 cggacaaatt gaaagatgtt tttcgtcatc atttatcgga tgaatccatc gaagttttca
    35761 cgaaggacta cagagaacta cgtgaattaa tcgaccaaaa tgatgaaact tattttgaca
    35821 agacaaaatt ggtcatcacg acttcggatc ttccacgttc cttttcaatt cctaatatta
    35881 atgtttatga catcttagac ggtgatggag cagccaattt ggagcatatt ttgagtcctg
    35941 aaatgaagcc ggcagaattt aaccgcttga ttcaggagct agtaaaattc ttttcgttgg
    36001 aaggaattgc ggaccgtttg aactttctga acccaacgat cgtgatgagt gaagtggaaa
    36061 cagtcatttc taaatatgaa aattactatc atttcacttt aaatggccgt gtaaaactaa
    36121 atctctatat gcatacggca ttgatgatgg aacgtttatt cttggctcgg acagaaaaat
    36181 ctgaagaacc gaattatgat ttgaaaccag atgaagatga attttattat gtttctagaa
    36241 gtatttttca atcaatggag atgaaatata attttaaagt caatgcttat gagctttcgc
    36301 tgctctatga attattagga ccgtatattc aaaaataaag tgtctaaaaa agtgtctaat
    36361 ctgttttttt taggttagac acttttttta atgtgaatta acgtgattaa accaatgttt
    36421 ttaaaagttg gcacaggaat tgcttatata aaagtgaaca gaaagaaagg aattaaccat
    36481 atgaagaaaa ttctaatagc gactcacggt cacttagctt caggctttct tagcagtatt
    36541 caattactga cggggaaggt tcaagagatt acggtgatta atgcttatgt ggatgagtgt
    36601 gattttgaac aggagttgaa tgtatttgta aaccaggtga acgagcagga tcagattttt
    36661 gtttttaccg atttatttgg cggaagtgtc aatcagaaga ttaccaaaac ctttttggag
    36721 aaagagattg ctgtaactat tttgtctggc tttaatctgc caattctgct cgaaatagta
    36781 ttggcaacca aggaattaac aaaggaggat attcaggaac tggtaaataa atgccaacag
    36841 gaattaaaga tgaccattct tgatgaggta catgtagtaa atgaagagga agattttttt
    36901 taggaggaag aaaaatgatt aaagcagtgc gaattgatga acgattaatt catggacaag
    36961 tagcgatgac ttggacaaag agtctaaatt taaccgggtt agtagtagca agcgatgagg
    37021 cggctaccaa cgatattcaa aagatgacat tgaagatggc tgcaccatcg aatgtcaaag
    37081 tcattattaa aacagttgat gacgcgattc atttgttaaa agatccgcgg gcgaaggaca
    37141 tgcgcttgat ggttttagtc cctaatgtca aagatgcggt aaaagttgcg aaagctttcc
    37201 caacggaaat cgaattaatg aacgtgggga atgctgggaa aatgacaccg ccatcggaag
    37261 ataagaaaac cttatcaaag gaagtgatgt taacgacgtc agaaattcaa gacttaaaag
    37321 aattagttga actttatcca gataccgttt ttcagcctac accggcgatg gaaaaacgtt
    37381 cagctatctc tattttgagt gatttttaga aaaaggaagg agaaaaatta tgtatgatgc
    37441 atttgtagtc gctttggccg tttttattgg aattgctgga catgaaatgt tcgggatgtc
    37501 catgctgagt cgtccgattg ttgttgctcc gttagcagga gcgttgatgg gggatttgca
    37561 gacggggttg attgtcgggg cgtcactaga agccatcttt atgggtgtcg tcaatatcgg
    37621 aatctctagt acggcggagc cttctttggc tgctgggctg gctacagcgt tcgcaatcaa
    37681 aatgggagga aacgtggatg cgattattcc cattgtcttt ccactagcag tattaggatt
    37741 acagttaatg aatatgattt tttcttttgt atgtggaccg atggctgcta aatttttcaa
    37801 atttgcaaaa tctggtgatc aaaaaggaat catcaatctg cacttcttta tgtggtttgt
    37861 ccactatgcg ctttatgctc ttattccatt ctttgccgtt ttatttggag cagatgttgt
    37921 caaaagcgtc ttggataata ttccagaggt catcatgaac ggattaacag tcgctggaaa
    37981 tctgctgccg gcagttggga tggcgatgct gttacggatg ctttggagta aaaatattgc
    38041 tgtgtggttt ttctttgggg ccgttgttat ggcgtatctc gagttaccat tgattgcaat
    38101 tgccgcagtt ggaacgatta ttgcaattgt tatagcccaa agagatctac aactaaaaaa
    38161 tatatctgtt catgcagttc aaagcgatgc tgctgtaaat gcaacgcttg accaagaaga
    38221 ggaggatttc tttcaatgaa attagctgat ggaatttcta aagatgagaa aaaattggtt
    38281 cggaagatgt tttggcgctc gatgactatg tatgcttcgg tgaatccggt gacgatgggc
    38341 ggcggtggtt tcgcctattc tatgatgccc ttcattaatc atttctacaa aagtgaagaa
    38401 gatcgagctg cggcgctgga acgtcataca cagtattttt cgacgaccat tccttgcgcg
    38461 tcctttgtca tggggattgc ggcatcgatg gagaaggaaa attctgaaaa agctggtttt
    38521 gacgtggaaa gtatcaacgc gatcaaaacg agtttgatgg gaccattggc tggaattggc
    38581 gattcattgt tctggggagt ttggcgtgtc gttaccgcgg gactagcgat caacttagcc
    38641 aatgccggaa atattttggc accaattatc tttttagtta tgttcaatat acctaattac
    38701 ttgatgcgat actatggcgg cgttttaggc tattcattag gctctaaata tattgaaaag
    38761 ctgtatgcga acgggttaat tgaaattctg acaaaagctg ctagtatctt gggtctgatc
    38821 atggtaggtg caatgacgat cagtatggtc aaattcaata ccgttttgag cttttcaatg
    38881 aagggcgaag aactaatgaa tgtccaagaa atcctcgata gtatttttaa aggaattgta
    38941 ccgttattgg taacattgag ctgtttcaaa ttactagata aaaaagtcgg cgtcatcaaa
    39001 attatttttg gaatggtcgt cttgggaatt ttattgtcat tacttggaat cgcatagtag
    39061 gaagctgcag ctactaggct gcagtttttt tagcaaggag ggaaaagatg actattaatg
    39121 aagtaattta ttcacccaaa cacccatttt ttaatgctga gttaaacgac ggaaatgtga
    39181 ctacttcaaa cgaatacagc tactatcgga atattcctga tgtaaaggga ttgaccttcg
    39241 atgtaagtaa tcaaaagaca ttagcaaacc ttgatgtttg cgggacgatt caaacgttga
    39301 ccttttatcg agaaaattta ctaactgagg aaaaaccagg tgtttgggtc aataaacagc
    39361 tctctcaaac caattcgtta tccttaaagg ttgaagtaaa cgggattgtc taccatttgt
    39421 cagaatcgga tcatcgtatt gaagtcgatg tgattcaaga ctgtttgcct agatatattc
    39481 atcattattc gacgtttaag gtcattgtag tatcctttgc tcccatactg gctaataagc
    39541 gtttaagtat gcttgttcaa caggtttaca ttgtgaatga aacacacgaa agccttcaag
    39601 tttcagtgaa agatgtgcct tcgtatcaag agaagtattc ggatcagcaa aatgtgttga
    39661 tttctcaaaa aggaaataag gacacgatcg cacaacagga agtggcaagc ttcgccattg
    39721 cttttgtgga cccaaatgct tttgaagaag taatgacttt tcaggaatct gatatcgaaa
    39781 aatggctgaa ggaaacattg aactattttg ctcagctatt tgggcagctg tcaatgccaa
    39841 acgatagagt tgtccatctc tatcgtcgtg cactttatca aagcttttct agtttcggta
    39901 tgaatcgaca gggacaggtc gtcggcagca attggggcag ctatcctgct actaatcgaa
    39961 tttggaataa ggatatgtat tacgcatcat tgccattcac catgtttgaa ccggagttat
    40021 gtcaaaaaac ggttctatgg tttgatcaat atggcgttaa gtttcctggt tcaaaattta
    40081 caggaggtat caatcattct ctgagtaatt cgttatccag tgcgttactg gctagtcttt
    40141 attttgaaca tactggggat acaaaattct ttgagcggtg tccgcaggtt ctactgaatg
    40201 cttcaagaat tattgaggac atattagctc aacgtcggtc aggggaacca atgctttttc
    40261 catctgtttg gttatcagac gcctttgcac ttggaaagta tcataccggc agcaatcttt
    40321 gtctttggaa ggcctgtatt ggacttgcag aaatttttga agccctagaa caactgagtc
    40381 ttagcaagcg atacaaaaac attgcctgta aactaaagga atcaatttgt aaacaaatga
    40441 ctatcgatgg tccttttggt gagcaatttt tggagggaat cggtgatgag gagaaaacaa
    40501 cctattcagt gaagcactat caaaaaccga ttcttgagca agggttaatc tttttaagtg
    40561 atgtgattgt ggatggaaaa attaatttat tgatgcatga tggagaagaa tcagacacta
    40621 cgttgatgcc tttttatcaa tttttagata ataaagatca tttatatcag aacaccatgg
    40681 ccttttctgc tagttctttc aatccaacct atagcgagga aatcaaaggc atcacttggg
    40741 gattagaatc cggcgccact tttcctggct ttatcactgt tttaatgagc gagctgcaca
    40801 accctcaagc tttcgtaacc cgattagaag aactaatttg tttggcagat ttagacggtt
    40861 cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg agaagtcgtg cgagactttg
    40921 gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag cctaatgatc agtcaatatt
    40981 tcggattgaa attcaaaaaa ggagtattgc aaattgatcc tgttgataat ttaacgtttt
    41041 cttggaagaa tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg caatccgagt
    41101 tgagtatttc aaatttgtca gaaaagccat tgaaaataag tgatacgaaa aaaattcttc
    41161 acgtagaaaa gggaaaaacg attcacattc aaaggagaaa aagatgagta tactaaaaaa
    41221 tattacccaa tttaaaaaaa tccagagtcg agcaatcact cccgaaaatc caacaggcga
    41281 gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca agccgcaaag gtcgtggcgc
    41341 aattccgctg acttctggtg aagaaactgt gattgcggat attactggaa cgggaataat
    41401 tcgtcatatg tggatgacga ttcgtgatcg aacagaattt ggcagttttg ttttacgtga
    41461 tttaatctta agaatttatt gggatgggtc ggatagccct gctgttgaaa gtcctttagg
    41521 ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg aactcgttgc cgatagtggt
    41581 taatcctaca gggggcttta attcctattt cgaaatgcct ttccaaacat ccgctaaaat
    41641 tacgatcgag aatttacatc cgaaggatat gtcctcattt ttttatacga taaattacgc
    41701 tttggtggac gaattggaag aaaatagcct atattttcac gcaaattggc atcgggaacg
    41761 cattaccaaa ttacaggagg attataagtt aattgatcga ttaaagggtc aaggctatta
    41821 cgtgggtact taccttgcgc ttacctgcct agaacgttat tggtggggcg aaggcgagtt
    41881 taaattctac attgatgggg atagtgagta tccgaccgta acctcaacag gttcagaaga
    41941 ttattttggc ggggcgtggg cctttcatga gtacgatgag ttcggacgtc cacatgcgaa
    42001 aaactattca acagcctttt tagggtatcc ttaccaatca aatagagatg ccacaagaga
    42061 tcgttttgaa acgggaaaat tgaatgccgt tcatgccttt ggtgacgacg caatgccacg
    42121 acatggtctt tatcgttggc atcttcaaga ccccatctgt tttaccgaag atttatcggt
    42181 cactttacaa caaatcggca acgatgatgt tcgcttattt gagcggcaag acgatgttgc
    42241 tagtgtcgct tactggtatt caacagaagc ttcaggaaat actaaacgtc atttcactga
    42301 tcgagaacgc ttgccgcgtt aatgaagtgt ttttcacata ttcgaaggga ggaacctatg
    42361 atgatatatg gggcaattga agctggaggg actaaatttg tctgtgcagt cggtactgag
    42421 gaattaacga ttttggaaag agtcagtttt cccacaacaa ctccagttga aacaatgaag
    42481 caggtcattg atttttttaa accttttaag gaacaattgg caggcatcgg ggtcggctgc
    42541 tttggtccaa ttgatattca atgcaaatca acgagtttcg gtcatatcac atcaacacca
    42601 aaacttgctt ggcaaaattt caattttgta ggaacgctaa agcaatattt caatattccg
    42661 attgaatgga caacagatgt caatgcggct tgctatggtg aatatgttgc tggttctggc
    42721 aaagggttag caggtgttgc atacttcact gtgggaacag gtatcggagg tggtgccttg
    42781 ttcaatggga ctttcgtgga agggttcagt catccggaaa tggggcatat gttgatcaag
    42841 cgacatccaa aggacaattt ttcaggaaac tgtcccttcc accaagattg tttagagggg
    42901 atggcagcag gaccagcgat tgaaaaacgg ttgggtgtaa aaggccagaa tttattggct
    42961 gacgattcgt tttggcagat tgaagccttt taccttgccc aatgtgcata caatacaaca
    43021 ctgatgtttt cgccagatag aattattttt ggcggtggtg taatgaaaca agaacacatg
    43081 aagaaaaaag ttcaggataa atttgttgag cttataaatg gatatgttga aataccacca
    43141 attgatagct acattattac gcccgaatta ggtgataatg ccggaataat cggcggatta
    43201 gcattggcca gaaaagcggt aagaaataag caaccataat taatgaatgt ttcattgata
    43261 ctcatgtcaa aagtatttaa ccgagtaatc aaatgatcaa atgaagttgg ataaagcgaa
    43321 aatgaattca aataaaaagg ctctaagtca aatcgtgttg ttttcattaa atcactgaaa
    43381 ggaaatgctt ctgcgtttcc ttttttcttt ttgttgtgcg ctgtatacct tgaggatatt
    43441 cactggtggc gtacacttaa atagtaatat attgtgttac tttatgtctg tggtaatagt
    43501 tatagttgct taccaatgta tgttcatttt tcgaaatgtt gacttcataa tccttggctt
    43561 aatgtttgaa tttttttggt gatagatttt ttcagccgta taaaata
//
LOCUS       DANJLF010000016        43369 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000016 DANJLF010000000
VERSION     DANJLF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(8..2389)
                     /locus_tag="PWT25_000979"
     CDS             complement(8..2389)
                     /locus_tag="PWT25_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL1021083.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="PWT25_000980"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="PWT25_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL1021084.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="PWT25_000981"
     CDS             3090..3629
                     /locus_tag="PWT25_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL1021085.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="PWT25_000982"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="PWT25_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL1021086.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="PWT25_000983"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWT25_000983"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="PWT25_000984"
     CDS             4736..5902
                     /locus_tag="PWT25_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL1021087.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="PWT25_000985"
     CDS             5948..7444
                     /locus_tag="PWT25_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL1021088.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="PWT25_000986"
     CDS             7525..8208
                     /locus_tag="PWT25_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1021089.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="PWT25_000987"
     CDS             8853..10043
                     /locus_tag="PWT25_000987"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL1021090.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="PWT25_000988"
     CDS             10145..11098
                     /locus_tag="PWT25_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL1021091.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="PWT25_000989"
     CDS             11167..12516
                     /locus_tag="PWT25_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1021092.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="PWT25_000990"
     CDS             complement(12699..13022)
                     /locus_tag="PWT25_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021093.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="PWT25_000991"
     CDS             13151..14314
                     /locus_tag="PWT25_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL1021094.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="PWT25_000992"
     CDS             14384..15733
                     /locus_tag="PWT25_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1021095.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="PWT25_000993"
     CDS             complement(15778..16209)
                     /locus_tag="PWT25_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1021096.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="PWT25_000994"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="PWT25_000994"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL1021097.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="PWT25_000995"
     CDS             19439..20035
                     /locus_tag="PWT25_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1021098.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="PWT25_000996"
     CDS             20157..21305
                     /locus_tag="PWT25_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL1021099.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="PWT25_000997"
     CDS             complement(21343..21756)
                     /locus_tag="PWT25_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL1021100.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="PWT25_000998"
     CDS             21835..22266
                     /locus_tag="PWT25_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL1021101.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="PWT25_000999"
     CDS             complement(22365..22598)
                     /locus_tag="PWT25_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL1021102.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22781..24451
                     /locus_tag="PWT25_001000"
     CDS             22781..24451
                     /locus_tag="PWT25_001000"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL1021103.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24598..24984
                     /locus_tag="PWT25_001001"
     CDS             24598..24984
                     /locus_tag="PWT25_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021104.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24971..25669
                     /locus_tag="PWT25_001002"
     CDS             24971..25669
                     /locus_tag="PWT25_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1021105.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25674..26138
                     /locus_tag="PWT25_001003"
     CDS             25674..26138
                     /locus_tag="PWT25_001003"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL1021106.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26135..27046
                     /locus_tag="PWT25_001004"
     CDS             26135..27046
                     /locus_tag="PWT25_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL1021107.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27281..27820
                     /gene="pyrR"
                     /locus_tag="PWT25_001005"
     CDS             27281..27820
                     /gene="pyrR"
                     /locus_tag="PWT25_001005"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL1021108.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27834..29117
                     /locus_tag="PWT25_001006"
     CDS             27834..29117
                     /locus_tag="PWT25_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL1021109.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29130..30056
                     /locus_tag="PWT25_001007"
     CDS             29130..30056
                     /locus_tag="PWT25_001007"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL1021110.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30075..31355
                     /locus_tag="PWT25_001008"
     CDS             30075..31355
                     /locus_tag="PWT25_001008"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL1021111.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31360..32436
                     /gene="carA"
                     /locus_tag="PWT25_001009"
     CDS             31360..32436
                     /gene="carA"
                     /locus_tag="PWT25_001009"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL1021112.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32441..35626
                     /gene="carB"
                     /locus_tag="PWT25_001010"
     CDS             32441..35626
                     /gene="carB"
                     /locus_tag="PWT25_001010"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL1021113.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35680..36456
                     /locus_tag="PWT25_001011"
     CDS             35680..36456
                     /locus_tag="PWT25_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL1021114.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36453..37385
                     /locus_tag="PWT25_001012"
     CDS             36453..37385
                     /locus_tag="PWT25_001012"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL1021115.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37382..38098
                     /gene="pyrF"
                     /locus_tag="PWT25_001013"
     CDS             37382..38098
                     /gene="pyrF"
                     /locus_tag="PWT25_001013"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL1021116.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38105..38794
                     /locus_tag="PWT25_001014"
     CDS             38105..38794
                     /locus_tag="PWT25_001014"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL1021117.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38767..39036
                     /locus_tag="PWT25_001015"
     CDS             38767..39036
                     /locus_tag="PWT25_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021118.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39074..39715
                     /locus_tag="PWT25_001016"
     CDS             39074..39715
                     /locus_tag="PWT25_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1021119.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39758..40450
                     /locus_tag="PWT25_001017"
     CDS             39758..40450
                     /locus_tag="PWT25_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL1021120.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40447..41349
                     /locus_tag="PWT25_001018"
     CDS             40447..41349
                     /locus_tag="PWT25_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1021121.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="PWT25_001019"
     CDS             complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="PWT25_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL1021122.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13875 a         7663 c         9440 g        12391 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22741 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    22801 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    22861 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    22921 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    22981 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23041 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23101 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23161 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23221 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23281 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23341 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23401 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23461 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23521 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23581 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23641 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23701 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    23761 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    23821 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    23881 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    23941 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24001 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24061 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24121 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24181 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24241 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24301 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24361 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24421 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24481 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24541 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24601 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24661 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24721 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    24781 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    24841 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    24901 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    24961 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25021 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25081 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25141 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25201 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25261 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25321 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25381 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25441 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25501 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25561 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25621 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25681 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25741 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    25801 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    25861 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    25921 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    25981 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26041 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26101 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26161 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26221 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26281 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26341 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26401 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26461 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26521 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26581 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26641 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26701 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    26761 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    26821 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    26881 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    26941 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27001 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27061 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27121 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27181 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27241 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27301 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27361 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27421 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27481 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27541 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27601 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27661 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27721 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    27781 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    27841 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    27901 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    27961 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28021 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28081 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28141 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28201 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28261 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28321 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28381 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28441 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28501 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28561 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28621 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28681 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28741 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    28801 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    28861 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    28921 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    28981 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29041 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29101 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29161 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29221 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29281 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29341 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29401 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29461 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29521 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29581 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29641 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29701 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    29761 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    29821 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    29881 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    29941 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30001 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30061 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30121 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30181 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30241 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30301 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30361 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30421 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30481 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30541 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30601 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30661 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30721 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    30781 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    30841 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    30901 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    30961 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31021 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31081 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31141 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31201 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31261 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31321 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31381 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31441 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31501 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31561 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31621 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31681 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31741 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    31801 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    31861 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    31921 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    31981 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32041 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32101 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32161 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32221 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32281 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32341 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32401 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32461 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32521 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32581 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32641 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32701 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    32761 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    32821 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    32881 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    32941 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33001 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33061 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33121 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33181 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33241 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33301 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33361 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33421 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33481 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33541 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33601 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33661 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33721 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    33781 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    33841 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    33901 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    33961 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34021 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34081 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34141 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34201 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34261 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34321 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34381 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34441 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34501 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34561 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34621 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34681 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34741 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    34801 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    34861 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    34921 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    34981 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35041 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35101 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35161 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35221 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35281 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35341 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35401 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35461 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35521 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35581 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35641 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35701 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    35761 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    35821 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    35881 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    35941 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36001 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36061 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36121 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36181 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36241 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36301 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36361 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36421 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36481 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36541 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36601 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36661 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36721 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    36781 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    36841 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    36901 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    36961 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37021 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37081 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37141 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37201 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37261 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37321 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37381 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37441 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37501 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37561 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37621 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37681 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37741 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    37801 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    37861 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    37921 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    37981 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38041 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38101 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38161 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38221 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38281 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38341 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38401 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38461 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38521 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38581 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38641 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38701 atggaaaaaa gatcctgaaa attggcagat tgaagcgcgc ttgatgaaga tagagaaatc
    38761 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    38821 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    38881 aaattcattt ttctaagact ggttagcaga aagaagagaa taatctcttc tcttttttct
    38941 attatatata gattttctct attttttggt aagggagctg tttcaaaaat ggtaaagagc
    39001 ggaaatgttg cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag
    39061 ggaggtaact tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat
    39121 cacaagggca cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg
    39181 tgtagcatct attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga
    39241 ttggaaactg ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga
    39301 tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga
    39361 agaaaagatg acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta
    39421 tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc
    39481 agataaggtt gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa
    39541 agtttataag gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc
    39601 ttctgttcag tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg
    39661 aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg
    39721 atatgagatg cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc
    39781 tggagacaaa ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga
    39841 atttccattg cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga
    39901 cacgctttcc tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat
    39961 tcatttagta ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt
    40021 gacagatatt catttccact tgccttctga acatatcatc aacggaaaac aggaagatat
    40081 tgaatttcat tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta
    40141 ccagctaatg gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt
    40201 agaaaaacat gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt
    40261 ccattatatt ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat
    40321 ggatcatgta ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg
    40381 accgaacaac cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt
    40441 tgagcaatga atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc
    40501 agagaagcgg caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa
    40561 ttagaaaccg agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca
    40621 gaagaagggc aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta
    40681 gaaactcact atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat
    40741 gatttcttag gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa
    40801 tttcggatca tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt
    40861 gagatcggca ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa
    40921 caaggagagc taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa
    40981 ggacatccat tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa
    41041 gttcgtttta cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc
    41101 cctgatcaag aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt
    41161 gaaactgatg cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg
    41221 cggttaatcc ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa
    41281 cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag
    41341 cagagctaag aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg
    41401 ggttgtgaca gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg
    41461 ctgttgtggg aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt
    41521 tcatagctgt aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa
    41581 taacataacc aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa
    41641 ccggcacttg ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg
    41701 taattgtttc atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt
    41761 ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa
    41821 gattgttttt accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag
    41881 atggtttctt acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca
    41941 attcttcttt aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag
    42001 attcaagata ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag
    42061 cgttcttcag cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg
    42121 ccggatctag tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt
    42181 cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt
    42241 aattctcagc gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat
    42301 ttcgttttag ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat
    42361 ctttctctgc tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg
    42421 ttacagcaga tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat
    42481 tttccgtttc gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc
    42541 atacaaccat cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat
    42601 cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa
    42661 gccgctgaaa gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg
    42721 gagcaacata ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt
    42781 gtttgatcgc atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac
    42841 gtcccattaa ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag
    42901 caaagtggaa atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc
    42961 catccaaata ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat
    43021 cgatttgggt aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt
    43081 ttttgccgcg tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg
    43141 agatccggcc agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac
    43201 catcaaagga catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa
    43261 aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta
    43321 attacataaa aaacagtgtt tctatcgttg aaatccaaat ttttaggta
//
LOCUS       DANJLF010000017        40414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000017 DANJLF010000000
VERSION     DANJLF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="PWT25_001020"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="PWT25_001020"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL1021123.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="PWT25_001021"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="PWT25_001021"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL1021124.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="PWT25_001022"
     CDS             complement(2303..2965)
                     /locus_tag="PWT25_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1021125.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="PWT25_001023"
     CDS             3149..4795
                     /locus_tag="PWT25_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL1021126.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="PWT25_001024"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="PWT25_001024"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL1021127.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="PWT25_001025"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="PWT25_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="PWT25_001026"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="PWT25_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="PWT25_001027"
     CDS             complement(8944..10023)
                     /locus_tag="PWT25_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1021128.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="PWT25_001028"
     CDS             complement(10126..10566)
                     /locus_tag="PWT25_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1021129.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="PWT25_001029"
     CDS             complement(10623..11033)
                     /locus_tag="PWT25_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL1021130.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="PWT25_001030"
     CDS             complement(11213..11647)
                     /locus_tag="PWT25_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1021131.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="PWT25_001031"
     CDS             complement(11747..12595)
                     /locus_tag="PWT25_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1021132.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="PWT25_001032"
     CDS             12984..14750
                     /locus_tag="PWT25_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL1021133.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="PWT25_001033"
     CDS             14852..16111
                     /locus_tag="PWT25_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1021134.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="PWT25_001034"
     CDS             16258..17559
                     /locus_tag="PWT25_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1021135.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="PWT25_001035"
     CDS             17562..18413
                     /locus_tag="PWT25_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1021136.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="PWT25_001036"
     CDS             18426..19241
                     /locus_tag="PWT25_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL1021137.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="PWT25_001037"
     CDS             19392..20558
                     /locus_tag="PWT25_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1021138.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="PWT25_001038"
     CDS             20788..21435
                     /locus_tag="PWT25_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL1021139.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="PWT25_001039"
     CDS             21519..22463
                     /locus_tag="PWT25_001039"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL1021140.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="PWT25_001040"
     CDS             22598..24049
                     /locus_tag="PWT25_001040"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL1021141.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="PWT25_001041"
     CDS             24070..24906
                     /locus_tag="PWT25_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL1021142.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="PWT25_001042"
     CDS             24906..25565
                     /locus_tag="PWT25_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1021143.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="PWT25_001043"
     CDS             25691..26380
                     /locus_tag="PWT25_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021144.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="PWT25_001044"
     CDS             26386..27342
                     /locus_tag="PWT25_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL1021145.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="PWT25_001045"
     CDS             27386..27898
                     /locus_tag="PWT25_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1021146.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="PWT25_001046"
     CDS             complement(28154..28702)
                     /locus_tag="PWT25_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1021147.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="PWT25_001047"
     CDS             complement(28791..29972)
                     /locus_tag="PWT25_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL1021148.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="PWT25_001048"
     CDS             complement(30208..31716)
                     /locus_tag="PWT25_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL1021149.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="PWT25_001049"
     CDS             31875..32066
                     /locus_tag="PWT25_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021150.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="PWT25_001050"
     CDS             32269..33186
                     /locus_tag="PWT25_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL1021151.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="PWT25_001051"
     CDS             33179..34252
                     /locus_tag="PWT25_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021152.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="PWT25_001052"
     CDS             34400..34918
                     /locus_tag="PWT25_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021153.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="PWT25_001053"
     CDS             34899..35291
                     /locus_tag="PWT25_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL1021154.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="PWT25_001054"
     CDS             35367..36302
                     /locus_tag="PWT25_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL1021155.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="PWT25_001055"
     CDS             36435..37214
                     /locus_tag="PWT25_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL1021156.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="PWT25_001056"
     CDS             37207..37524
                     /locus_tag="PWT25_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL1021157.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="PWT25_001057"
     CDS             37768..39378
                     /locus_tag="PWT25_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1021158.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="PWT25_001058"
     CDS             39535..40383
                     /locus_tag="PWT25_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1021159.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DANJLF010000018        39635 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000018 DANJLF010000000
VERSION     DANJLF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..294)
                     /locus_tag="PWT25_001059"
     CDS             complement(<1..294)
                     /locus_tag="PWT25_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021160.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYG"
     gene            complement(468..1193)
                     /locus_tag="PWT25_001060"
     CDS             complement(468..1193)
                     /locus_tag="PWT25_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL1021161.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1246..1722
                     /locus_tag="PWT25_001061"
     CDS             1246..1722
                     /locus_tag="PWT25_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021162.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(1728..1910)
                     /locus_tag="PWT25_001062"
     CDS             complement(1728..1910)
                     /locus_tag="PWT25_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021163.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(1910..2209)
                     /locus_tag="PWT25_001063"
     CDS             complement(1910..2209)
                     /locus_tag="PWT25_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021164.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2314..2517)
                     /locus_tag="PWT25_001064"
     CDS             complement(2314..2517)
                     /locus_tag="PWT25_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021165.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2532..3278)
                     /locus_tag="PWT25_001065"
     CDS             complement(2532..3278)
                     /locus_tag="PWT25_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL1021166.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3356..3664
                     /locus_tag="PWT25_001066"
     CDS             3356..3664
                     /locus_tag="PWT25_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021167.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3635..3892)
                     /locus_tag="PWT25_001067"
     CDS             complement(3635..3892)
                     /locus_tag="PWT25_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HDL1021168.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4190..4330)
                     /locus_tag="PWT25_001068"
     CDS             complement(4190..4330)
                     /locus_tag="PWT25_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021169.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            4630..4950
                     /locus_tag="PWT25_001069"
     CDS             4630..4950
                     /locus_tag="PWT25_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1021170.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            4968..5399
                     /locus_tag="PWT25_001070"
     CDS             4968..5399
                     /locus_tag="PWT25_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL1021171.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            5526..6110
                     /locus_tag="PWT25_001071"
     CDS             5526..6110
                     /locus_tag="PWT25_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL1021172.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            6212..7360
                     /locus_tag="PWT25_001072"
     CDS             6212..7360
                     /locus_tag="PWT25_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1021173.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(7449..9074)
                     /gene="groL"
                     /locus_tag="PWT25_001073"
     CDS             complement(7449..9074)
                     /gene="groL"
                     /locus_tag="PWT25_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL1021174.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(9125..9409)
                     /gene="groES"
                     /locus_tag="PWT25_001074"
     CDS             complement(9125..9409)
                     /gene="groES"
                     /locus_tag="PWT25_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL1021175.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            9634..10296
                     /locus_tag="PWT25_001075"
     CDS             9634..10296
                     /locus_tag="PWT25_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL1021176.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(10372..11664)
                     /locus_tag="PWT25_001076"
     CDS             complement(10372..11664)
                     /locus_tag="PWT25_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1021177.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            11834..12463
                     /locus_tag="PWT25_001077"
     CDS             11834..12463
                     /locus_tag="PWT25_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL1021178.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(12566..13375)
                     /locus_tag="PWT25_001078"
     CDS             complement(12566..13375)
                     /locus_tag="PWT25_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1021179.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13430..14299)
                     /locus_tag="PWT25_001079"
     CDS             complement(13430..14299)
                     /locus_tag="PWT25_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL1021180.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(14300..15616)
                     /locus_tag="PWT25_001080"
     CDS             complement(14300..15616)
                     /locus_tag="PWT25_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021181.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(15613..17448)
                     /gene="walK"
                     /locus_tag="PWT25_001081"
     CDS             complement(15613..17448)
                     /gene="walK"
                     /locus_tag="PWT25_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL1021182.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(17453..18157)
                     /locus_tag="PWT25_001082"
     CDS             complement(17453..18157)
                     /locus_tag="PWT25_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1021183.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(18347..19207)
                     /locus_tag="PWT25_001083"
     CDS             complement(18347..19207)
                     /locus_tag="PWT25_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1021184.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(19194..19763)
                     /locus_tag="PWT25_001084"
     CDS             complement(19194..19763)
                     /locus_tag="PWT25_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL1021185.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            complement(20337..21506)
                     /locus_tag="PWT25_001085"
     CDS             complement(20337..21506)
                     /locus_tag="PWT25_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1021186.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            complement(21550..21780)
                     /locus_tag="PWT25_001086"
     CDS             complement(21550..21780)
                     /locus_tag="PWT25_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021187.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            21856..21928
                     /locus_tag="PWT25_001087"
     tRNA            21856..21928
                     /locus_tag="PWT25_001087"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21889..21891,aa:Lys,seq:ctt)
     gene            22090..22311
                     /locus_tag="PWT25_001088"
     CDS             22090..22311
                     /locus_tag="PWT25_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021188.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(22635..22880)
                     /locus_tag="PWT25_001089"
     CDS             complement(22635..22880)
                     /locus_tag="PWT25_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021189.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(22874..23275)
                     /locus_tag="PWT25_001090"
     CDS             complement(22874..23275)
                     /locus_tag="PWT25_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1021190.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(23447..25255)
                     /gene="uidA"
                     /locus_tag="PWT25_001091"
     CDS             complement(23447..25255)
                     /gene="uidA"
                     /locus_tag="PWT25_001091"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL1021191.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(25283..27049)
                     /locus_tag="PWT25_001092"
     CDS             complement(25283..27049)
                     /locus_tag="PWT25_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL1021192.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(27062..27856)
                     /locus_tag="PWT25_001093"
     CDS             complement(27062..27856)
                     /locus_tag="PWT25_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1021193.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(27867..29243)
                     /locus_tag="PWT25_001094"
     CDS             complement(27867..29243)
                     /locus_tag="PWT25_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1021194.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(29372..30019)
                     /locus_tag="PWT25_001095"
     CDS             complement(29372..30019)
                     /locus_tag="PWT25_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL1021195.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(30103..31530)
                     /gene="uxaC"
                     /locus_tag="PWT25_001096"
     CDS             complement(30103..31530)
                     /gene="uxaC"
                     /locus_tag="PWT25_001096"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL1021196.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(31604..32683)
                     /gene="uxuA"
                     /locus_tag="PWT25_001097"
     CDS             complement(31604..32683)
                     /gene="uxuA"
                     /locus_tag="PWT25_001097"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL1021197.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(32686..34317)
                     /locus_tag="PWT25_001098"
     CDS             complement(32686..34317)
                     /locus_tag="PWT25_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL1021198.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            34634..35305
                     /locus_tag="PWT25_001099"
     CDS             34634..35305
                     /locus_tag="PWT25_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1021199.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(36141..37097)
                     /locus_tag="PWT25_001100"
     CDS             complement(36141..37097)
                     /locus_tag="PWT25_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021200.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(37206..37451)
                     /locus_tag="PWT25_001101"
                     /pseudo
     CDS             complement(37206..37451)
                     /locus_tag="PWT25_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37596..38018)
                     /locus_tag="PWT25_001102"
     CDS             complement(37596..38018)
                     /locus_tag="PWT25_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021201.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(38198..38569)
                     /locus_tag="PWT25_001103"
     CDS             complement(38198..38569)
                     /locus_tag="PWT25_001103"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL1021202.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            complement(38903..39166)
                     /locus_tag="PWT25_001104"
     CDS             complement(38903..39166)
                     /locus_tag="PWT25_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021203.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT        12038 a         7870 c         6068 g        13659 t
ORIGIN      
        1 tccgtaataa gagatatcaa tcaagttatc tctgttgtac tcttctagcg catcaatcaa
       61 gccatcttcg atgacataga tgtattcagg tttatttgaa tgttttgata gatggataag
      121 gtaaacatga tcccaaatac tcacaaaatt tcccaagtca tcttgatcac atgatagttc
      181 ttcgttcgtc aaaagatttc gtctgatttt tcgattattt gtttccttga tattcgactt
      241 gccccattca gaatcagtca aatattgatc taaagtggaa agttctttat tcatatgcta
      301 aaattctcct tagatatgtt ttgtgactct atgcttgccg gctgagtcac ttttttattt
      361 gttgccatgc tttttgcttt tcgatatgtt gttggcttag gatgtttggt ttattgtgtc
      421 tccaccagcg attagcaatt accgtcccta ttcttagcgc ttcagctcta ttcattttca
      481 tcaccgaaaa gtctttgttg tctgttcagt tgatcgattt ccatgcggat cgcagtttct
      541 ggtaaccaca tttcaataaa tgaaacagca tcatcgaatc tcttacgagg taactcgcca
      601 tatcttggga ttgaaaaggt acgtttaaat tcagaccaaa attttgagaa tacttttttg
      661 ctgatttctt cataagctcg gctttctttt ccccctagaa cttccataac tttcatattt
      721 cctttttgct taatttcaaa ctcttgttgt ccgctaattc gcatagtatc tttaagcatg
      781 gaaacatctt ttttaacatc tttcatttct tctaattgat agatcatcat gtcttcaatt
      841 gtttgaggaa cagtattctt ccgaataaca tcttccattt cgttgaaagc ttcaataaat
      901 tttagtttga aatttattgc tttacttcca gtgaatccca tagctagcaa ggaaaatccg
      961 tctctattca tgaaataaac tctccgactt cttccgtatg agtctggctc gttcccttcc
     1021 acaaacatct gtccaaaatt ggacccatct tcaacattgg ccgaattttc gaccgatctt
     1081 tttattgatt caattgcttc tagtacatgc ttatgtttct ttttgaaact ttctgccact
     1141 tgtaagctcg tagttacagc ttctttattt ttcaaaatta ctaattcttg cattatttct
     1201 tctctccttt ttggtataat tttcgttaga aagtgaggtg attctatgag gaaaaagctc
     1261 aattacattc ttactgcgac tttaaccata tatataattc tgtttattac cttatctttc
     1321 ctgtcttcaa ttaaaaaaat tccttttatt acattgaata aaaatagtat tttgaaggag
     1381 ttcaataaca ctgactacat cgcatttcta aaactttttt cagtctgtgt gctaatatca
     1441 tgtgtaatta tttttattgt tgctaaaaca tgttggacaa tgttaaataa aaaccaacgc
     1501 gatgttgaaa atattgctat atttttaatt aagattactt cctcaatatc aattatcatt
     1561 ccgatgactg taactttttc aaaagaacag tttaatgtag cggctactta tctttcattt
     1621 atagcgctat tttcttttgt tcttcctaaa agaaataaga gcaactttga caacaaggag
     1681 ctggatgaag tacgcaatag cagcccaaag gaacaaaaat aaaaaaatca tattttagtc
     1741 agtccctccc gactggcttt tttcgctctg tgctcagctt catcaagccc cataaaaatc
     1801 caaaccatgt aaacgattgt ccctatcaac gcttgtctgc ttccccagag gcctaaagcg
     1861 tagatgatta gcggtgcgct gaatactagt gctctgttga atttgcccat taacttgcct
     1921 ccttcaaaac atctgaaggc gataaacttt ttacacgata tttgtttgcg tctttccact
     1981 ttaaaaacca aataaaagta tggtaatgaa taaatgtggt tgagtgacct ggacgaagta
     2041 ttccttctgc aaattcagga attgtttcca tttccttgca gtattcaact aaagtagttt
     2101 ttgacatgcc gtggaacttc tccaagatta aactttgtcg gtaccagtcg tctggtttta
     2161 ctgccttctc agcagcttct atcagctctg aaagtgttgg ttttttcact tattatccct
     2221 cctaaaaatt atcgttccat actcgatgtt taacatttct tgaagctctg attttgtaag
     2281 aatgatttta tcttccaacg actttacctc cctctacaat tcgtacatag tgataatcga
     2341 atctataatt ctgtttgctt ctgcagaagt ctttttaccg tttaaaatta aagataagta
     2401 gcttttgcta attccaaatc tttcagcaag catggtgtaa gttaagaact ttgaactttc
     2461 gacatattct ttgatttttt ctctatctcg ttgagtgatt tctgcaatgt cagtcatact
     2521 aaaactcctt tctaaccaat ttcctctaaa tccatttgag ggtaatagcc ctctgcgatt
     2581 aataagttgt aaataaacac tcgccctttc tgtgtccatt tggtattcat tacaacttta
     2641 gtgccgccat cggctttcgg tatctcactt gtgtgcgatt ttgtgtatcc ttggttcata
     2701 tgttttcggt aaagtatcca ttgtccgcta actttatgct gaacacctaa ctcgtttagc
     2761 aatttgttta gagcaattgc cgacattccg taatcagctg caatttgaga agtagctacc
     2821 gtatctgtcg atgaaagaat cgtatctaag tagctaatct ttggttcgta ctcggcaatt
     2881 tgttgttcga gtaattggtt cttttcttct aaatccgctg ctaaacgcaa tgcttgtgca
     2941 aaactttgag gtacgtttga atagctacct gtttttctaa ttgttggaag gacttcactc
     3001 gttacccaac gtttaaattt tttggcagag ggaagttttg attttaagat taaactgtaa
     3061 aggcctgact cgttaatcaa aacaactgct gttccattga cggtgaacga atcgttctgc
     3121 gtcttatcct ccaagtccac atgatctcga atagcttttt gcggattcga atatcctaga
     3181 acgtctgcta catcttttcc tacaaaatat ggttcatcat ttactaaaat agttcgaact
     3241 tcgttttgct cgaaattaaa aatttgtggt gtattcatat ttctcattcc tttctttggt
     3301 ataatttcct tatcagtgtg acaggctgaa ataattgtta aggaggtgaa aatttatgaa
     3361 accagaattt gtaattacta tgcttgatgg agaaaaaatc aaaatatttg ccgatacttt
     3421 ggtcgttgga tacgatgatg cgtcaaatat cgaagaaaaa gatggaatgt tctattcttc
     3481 tcaaatttac gtgaatacct tgcaaggtga tattggggta tctactcttg caacatcaag
     3541 accgcttgta ggtcttatcg gctttttgtc aaaagcaaca ttcttttctc tcggcgatga
     3601 tatagattcg gatgaaactg tcatctataa aacatcagct gttaaaagta tcggtttcaa
     3661 atagtaattc ttgctcgttg atagcggaag ccaacttttc ttccaacaca tcggcgagct
     3721 ttctttttgt tggattcatt tcaaagttga tagtcttgag aagcattttt gcatccttaa
     3781 ctttttgtcc ttgcaattcc ttgatgattt tatcggcttg gctatcaatt ccatgtgtta
     3841 gatcatctat cattttttgt accttgattg tttggctcat ttccttgttc attgtttagc
     3901 ctcctttaat aaatgatttt ttgtattttt taatttccat aaattcctca aattgctctt
     3961 tggtattatt accacgacca tacatattgt gaaattcttt atggcaactg acacaaagtg
     4021 taacgccgtt tgaaatcgct gcggatttag ttgttgcatg gctaaatcac gaatcacaag
     4081 tatctactaa aggcgaatca atttcgccaa aagaaattgt tcaagcttat ttagatattc
     4141 attatgctgt tatctatggt cagcttccag aagaccgtaa aaacgatgac taaacgtgag
     4201 ctaaaatctc tgctatggct gcaaccatgg cagagtcttc tttatctacc gcgtgttcca
     4261 tcgccacttt tgcttgtttc agtatgtcta atttcaaatc ttcgattttt gccgagactg
     4321 ttttttccat attcttctct cctttctttt atattcgtaa acaaatttaa caacttttta
     4381 aaaaactgcg ttgactttat tagattaata atctatacta aatgcatagt taaataagac
     4441 atataaactt tgatttaaaa agctttcttg gcggttggca tttattaatc aatagtattt
     4501 tttgttgtct ttttagttgt taaacttgtt tacaaaacat agtatagatt aataatgtat
     4561 ataagtcaac tatttttaca ttatttatct aaactttttt tgtaagcatt cagaaaggtt
     4621 gatttatcaa tgaatactta cgaaataata aaggagttag caaaagaaaa aggattatcc
     4681 attagacagc tcgaaatgaa tttcgggtat tcgaatggat atttaggaag ttggaaaaga
     4741 caaactccta attctattga gttagctcgg ttagctgatt actttggagt ctctgtagac
     4801 taccttctag gtagagaaga aagagaaacc cctaaacatg tggatttatc agaaaacgat
     4861 actgtttttt cttttgatgg aaaagaaata tctaaggaga caatgcgtaa agcgattgca
     4921 attgctaaag ctttagagga aaatgaatag ttggagtgat gggttgtatg tatttgaaat
     4981 tgaaagaaat gttgagcgaa tacaatttga aagtagttta catggaaatg aaagagcctg
     5041 gcttctatta tccgaagcct agaatcattt ttttgaatga aaatctatat ggcgaaactg
     5101 cagaagcctt tcatttatct catgaacttg cgcatttcac cgcttctcat tttgaatatt
     5161 cagttttgta cgatacttct acaacttttc actcaaaatt tgaaacagaa gcagataaaa
     5221 tagctattct aattttatta aatatctata ttgagaacga actgactgat gaatctcagt
     5281 ttaatctcga aaaatttatg gagtattatt ctattcagaa taagctcaga tacacttgtt
     5341 atgctgtctg ccaatgctat tttaagaaaa aatattctta cgcaaggcaa tatgtatgaa
     5401 tacatcaaga attattaaaa aaaccgattt cagcaaaaca ttaaaaaagc ccgtgctggc
     5461 acacggactc atacctcatt tctgagatcg caaatatatt ataacaagaa gtgaggaata
     5521 tttaaatggc aaaaaaagtt atgggtcaag acggaaaaat gtataaagtt agtaaacctt
     5581 tttacaaaaa agtatggttt tgggtattag cagtaatttt aattataatt attggttctg
     5641 ctttaaatgg tggatcagat agtaataaag caagtgataa tggtggcgaa aaagtaacta
     5701 aatcttcaac ctctgcttca tcttctaaag aagaaaaaag cgataccttc tataagattg
     5761 gtgacactgt aaaagttggt gatgctgaat atacactaaa tagtgtagaa ctaactgatg
     5821 aaagaaatca atttgaagaa aaccaacctg cacaagtagt aaaaattact tataccgtaa
     5881 aaaatgatgg cgattcagat atccctgtag gtacagatgt ggaagtatat ggaccagatg
     5941 acaaaaaatc agagacatat gccaacgaaa atacaatggg atctgttgct ccaggaaaac
     6001 aaatggatgt aactgctcat ttcacgttaa accaaaaggg agaaatagaa atccacttct
     6061 ctcctttagt atcatttgaa aaagcagcta tttttaaagc aactgtataa taaataaaaa
     6121 acacgcccca ccgtccagag taagagcgtg cttttagaaa acaaacctat ataggtctat
     6181 ttgttatgcc tattatagca aatgatagga gatgttaaaa gtgtgggttg aacagaccaa
     6241 agatggccaa ttcaagttta ttgaaagata tgtagatcct tatacagaaa aaacacgaaa
     6301 aaaatctaca acacttacaa gtaattcacc acaagcgtgg aaaaaagctc agaaaattct
     6361 agataaaaaa attaaagaag cactcgaaga ttacaataaa tcagatatca cttttggtga
     6421 gctgtataaa gaatggtatg aatattataa gcagcatgtt aaacgtacta gctatttgaa
     6481 ggttccaatg atgatgaaac atgtttctaa gcacataagc gatgatacga tcgttagaaa
     6541 cattgatgag acactcatta ataagataat tgaagatatg tatacgtttg gtgacctctc
     6601 actgaactac acaaaacaaa caaaaacaac tctatctgtt atgctaaact atgcaatcga
     6661 tagaaaatac attcaaagaa accctgcgct agcagttaaa atccatccta aaaaagtgga
     6721 agaagaaaaa agaaggcttt ctatgaataa aaaatatctg gagaaagaag aaattgatca
     6781 aatactgaaa cagctatact ccaatcctcg cagaaaacta cacggcataa tagctgagtt
     6841 tctatattta actggtttga gatatgggga attgctagct ttgcaaatga aggactatga
     6901 ggatgggaaa atttccatta atgggacctt agattacaca tctgtgaaaa tggataatgc
     6961 tataaaaaca actccaaaaa atacttattc gcaacgtgaa gtgcaattac ccaatcgtgc
     7021 aaaagaattg attgaaagtg tgatagctga caatattctt gcaggtagac ccacagatcc
     7081 cgatcaatat atatttatat ctacaagtgg cactccgctt gcgctgcact catttaacgc
     7141 tatactccat aaagtagagg aagagttgga attagaaaaa agtctatcct cacatatatt
     7201 cagacatagc cacgtttcac tattatctga attaggcgta cctcttaaag ccattatgga
     7261 gcgtgtaggg cattctgatg caaacacaac tctgtctatt tataaccatg taacaaaaag
     7321 agccaaacaa caagtaattg ataaactaaa tagcctttga tatttttgcc ccttctttgc
     7381 cccttttgct taaaataaaa caaaaaaaga agaaagaacc ctgttaaaat agggttcttt
     7441 cttaattact acatcatacc gcccatcatt gatggatcca ttgcaggagc tgctggcgct
     7501 gctggttctg gtttatctgc aacgactgct tctgtcgtta ataacaaggc agaaacggaa
     7561 gcggcatttt gcaaggcaga acgtgttact ttagttgggt ctacgatacc tgcttcaacc
     7621 atgtttaccc attctccagt agcagcattg aaaccagtgc ctagttccac gttcttcaat
     7681 ttatcaacaa tcactgatcc ttcataacca gcgttttcag cgatttgacg aattggttct
     7741 tctaaagcac gaacaacgat cttgatacct gttgccacgt ctccttctgc ttctacagca
     7801 gaaactttgc tgataacgtt cactagagct gtaccaccac ctgagaccat tccttcttct
     7861 actgcggcac gtgtagcatt caatgcatct tcaatgcgta attttaattc tttcaattct
     7921 gtttcagtag ctgcaccaac tttaacaaca gctacaccac ctgctaattt agctaagcgt
     7981 tcttgtaatt tttcgcggtc aaaatcagaa gttgtttcag caatttggtt tttgatcaat
     8041 tgcacacgag cttcgattgc ttctttttct ccagaacctt ctacgatcgt tgtattgtct
     8101 ttatctacaa caactttaga agcatttcct aagttttcga ttgttgcatc tttcaattct
     8161 aaacctagat cgtcagtaat tactgtacca cctgtcaaaa tagcgatatc ttcaagcatt
     8221 gctttacgac gatcgccgaa accaggagct tttacagcta ctacgttgaa tgttccacgg
     8281 attttgttca atacaagagt tggcaatgct tcaccatcta catcgtcagc aataatcaac
     8341 aatggacgtg attgttgcaa gatttgttct aacaatggca gaatatcttg gatgttagag
     8401 atttttttgt ctgtgatcaa gatatatggg ttttctagaa cagcttccat tttgtcgtta
     8461 tctgttacca tatattgtga taagtaacca cgatcaaact gcataccttc tacaacatct
     8521 agttctgttt cgatcccttt tgattcttcg attgtgatga caccgtcgtt tccaactttt
     8581 tccattgcat cagcaatcaa atgtccaact ttatcagaac ccgaagaaac agctgctact
     8641 tgtgcaatgg cttcttttga atcaacaaca gttgagatat tatgcaattc ttcgactgct
     8701 gcttttgttg ccaattcgat cccgcgacga atgcctagag gattagcacc tgcagttacg
     8761 ttttttaaac cttcgcgaac gattgcttgt gtcagaacag ttgctgtcgt tgtaccgtct
     8821 cctgcaatat catttgtttt tgatgctact tctgatacaa gttttgcacc catgttttca
     8881 aaatgatctt ctaattcgat ttcttttgcg atcgtcacac catcatttgt gatcaatgga
     8941 gagccgtaag atttttctag tacgacattt cggcctttag gtcctaatgt tactttcact
     9001 gtatctgcta atttatctac cccgcgtaac atagctgcgc gtgcgtcttc tgcaaatttc
     9061 aattcttttg ccatgattga ttcacctcat tgattatttt tgattatttt tttatcttcc
     9121 aaatttactc aacaatcgcc atgatatctt ttcctgcgac gatcagatat tcttttcctt
     9181 cgtattttac ttctgtaccg gcgtattttt cgaacataac ttggtcgcct gcttttacag
     9241 cggcaggaac tttttcaccg ttttctaaca aacggccttc accgactgca acaactgtcc
     9301 cagtttgggg tttctcttta gcagcagaag ctaaaacgat tcctccgaca gttttttctt
     9361 cttcttttgc gacttcgata atgacgcgat cacctaatgg ttttaacacg ataaatccct
     9421 ccgataactc tttttttaat ctgttagcac tcttaacctt cgagtgctaa cctacatttt
     9481 taataatact cattctccct cttttttgca agcttttaaa tcaaagaaaa gaaattttat
     9541 tctgacagct gcttattgaa attatgatat aattgaaatc aatcgatata agcttgcctt
     9601 gcatgcttgt tatcccttaa aaggaggaat tacatgtctt taaaaaaata tagcttcctc
     9661 agcatttttt gctatggtct aatttttatc tcacccttga tcctagcttc gataggactt
     9721 gtaaaatcta cgtctgaatt gatcacagca actgcagtca tgtatatact aggcgccgtt
     9781 gtcctagcta ttttttattt taagcaacgt gaacccttag cgatcgaatc agcggtaaaa
     9841 aaagcttctg ctccaaagat tgttatttat ggattggttg gaatctttgt cgcattgatt
     9901 ctgcaaagca tcgctgtcat gttagaatct gtattgtttg gcaaagcaac cccttccgag
     9961 aatacacaaa atatcatcca gatgatcatg gaagcacccg cttttatttt ggcgacaact
    10021 gttgctggac ccatcatgga agagttcgta ttccgccgca gtatactggg gatcatcagt
    10081 cgatattcaa atttttgggt cggagcagtg atcagctctt tgctgtttgc gtttgctcat
    10141 aatgatggtc atctgctaat ctactttttc ctaggtttct tcttcagcct agagtacaaa
    10201 gcaactggtc ggatctggac atcgatgatc acacacgtag gaatgaacac attagttgtt
    10261 cttgttcaat tagctgtcca aaaagggcta gtataaacat gactataaat actcctgtca
    10321 tcctagattg gtgattttcc caatctaaag cgacaggagt atttttgttt tttagtcgtt
    10381 catcttttct gctgctccat cattttttgt ctgtctttcc tttttgcagt ttgcagaatc
    10441 tatttttttc aaaaatggga aataaatgaa aaaactaatt gacaacacga taccttgtaa
    10501 aatcgctgca ggcactccgc ctactaagaa acccgagagg atcggcggtg ttgtccaagg
    10561 gacttggact gctgtaaata aaggaacgaa tccaaaatat aaagcggaat aggtaatcaa
    10621 tcctgaaacg accggtgcga aaataaaagg aacagccatc atcgggttca tcacgatcgg
    10681 tgtggaaaaa ataattgatt cattgatgtt gaaaaaggct ggtaaaagag caagatttcc
    10741 taattctctg tatttcttcg atttcgcaaa aaccgtcata aaaaatacag cccctaacgt
    10801 caatccagca cccgtaaccg tcatgaactg atcgagaaat tgctgagtaa cgatatgtcc
    10861 gccatttgcc accgtcaact cttgtcctga ggctaagatt tctgcatttg ctaatgaatt
    10921 cgcttgcaag acgggactaa ccaacccatt gaccatcgca gatccatgaa gaccaaataa
    10981 ccataaaaac ggaacaaaaa aacctaataa aagcacacca aacaacgtgt ctatgaacga
    11041 ttgaatggga agctggacat atgtccatac tccttgaaca acagtcattc tgaacaaatg
    11101 ttgaaaaacc gctgaaccga taagcgtaaa agtgactata acaatgattg gaatcaatga
    11161 agtaaaagac tgccaaacat tttcaggtat cttctctctt tgcttccacc ctttcgcaag
    11221 tcttactaaa cgtgcatagc cccatccgat gatgagccca gttgtaactg ctcctagtat
    11281 cccttcagca cctgtccaga cagtatctat ctgagtattt agtggctgaa taagcagtag
    11341 ataagcagac aaggctgtta tgccgactgg taagcgatcg atgtcttctt cttttgcata
    11401 agcgtatgca atggcaaaaa cgatccaaaa ggctatcaca tcaaatgtag cctggtatac
    11461 ttgtgtaaaa tacatagaaa atcctgtttt gtctatccac tgagcgatgt aatccacagg
    11521 aaaatgagca aataagaaaa acagcgagcc aacaataatt attgggatcg tgtaagtcat
    11581 tccattacgc aaagcagtta agggtctaag attcacaaca gatgttatca ttggtaaaaa
    11641 ggcacgatct aaccactctt tcatttctta tctccttcaa cttctatttt tatcaatcag
    11701 ttttcttaat ttaactatac ccaatgatca cgaaccatga aaggaatgcg cactgagctt
    11761 ttcaaaaatc ggattctttg acgaatattc agcaactgct tacaatggac aggaataaaa
    11821 aaggaggttt tttatgaaaa ttgcactcat tggagctact ggccatgcag gttcatttat
    11881 tttagacgaa gcattgtcca gagatttaga cgtgacggca atcgtgcgtc atccagaaaa
    11941 attgccatca gatgttccgt ttatacaaaa ggatttattt gagttgacaa ctactgatct
    12001 tgcatctttt gatgttgtcg ttgatgcttt caacgctccc aaaggaaaag aagaaatgca
    12061 tcaaacttct ttgtcccatt tgattgagat gcttgaagga accgatacac gcttaattgt
    12121 cgttgggggt gcctcttcgt tattcttaga tacagaaaag acgactcgta tgattgacca
    12181 agtacctaaa aatgcaccct tctatccaac agctttcaat atgtacgaag gattgttaaa
    12241 attgaaacaa tctaaaaatc tacattggac ttatatcagt ccagcttctt tatttgatcc
    12301 acatggcaaa cgtacaggta cttatttgtt aagtgatgat tatttacaaa gaaacgctgc
    12361 tagcgacagt acgatcagca tggcagatta cgcgattgca ttagtagatg agatactgga
    12421 tgagaaacat gaaaagcagc acatcagtgt agtcagtcga taatacggtt atttttttaa
    12481 tatgctaaaa aaataaaaat agaagataaa accaaccctg attgatttta tcttctgctt
    12541 tttatatttt tattgattga actgtttaaa tcgaaaatag ctcagttgct tcatctggat
    12601 ctgtatcata gatttcaaaa tcatgtccaa cgccaaaatc tttttctttc aaaacatttt
    12661 ccattgttaa atgagcaagt tctttcatat tgttttcctt gctaagatgg ccaagataga
    12721 ttcgtttggt ccgattaccg atcacatcag tcatgacaag tgcaccgtct tcgttggaaa
    12781 gatgtcctct atctcctaaa atacgctgtt ttaaattcca cggataaggt cccatgcgta
    12841 acatctcaag atcatgatta ctttccatca aataaccatc ggcattttca atgacacctc
    12901 gcatataatc actgcaatat ccggtgtccg tcaacatgac gaatgaccga ttttctttat
    12961 ggaaacgata aaattgcgga gccgctgcgt catgggagac gccgaaactc tcaatatcga
    13021 tatcgccgaa tgttctgact ttccccatct caaatagatg tttttgttcc actggtacat
    13081 ttccaatcag tggttccatc gcttcccaag tctgctgatt ggcaaaaaca tccaaatgat
    13141 acttacgagc caagacaccc acgccatgta tatgatctcg atgttcatgc gtcacaagta
    13201 ttccatctaa ttggtccggt gtccgatcaa tttgagccat caaggaagtg atttttttac
    13261 cgctaagtcc tgcatcgacc aaaagccgtt ttttttcact ttcgatataa agtgaattac
    13321 ccgaacttcc acttgctagg atactgattt taaaagctgg tttttcttca gccatttgac
    13381 tcctacttcc cttcttactt tgtaaacttt tttcattata ctcgcttttt tattgatctt
    13441 ccacttttgg tacagtattg tttgtcacga tcgtattgct gaaagcgttg acgctctcaa
    13501 tttggaccac tttatcgctt gtttcgatcg ctacatgcca aacaggaaca tacacatttt
    13561 tttcccggac tttcagaata caggaataag ctagctgacg ccataaaatt tttgatcctc
    13621 ttggtatttt gttattgatg tacaatgtat tgattgcttc ctcttccgta tacagttccg
    13681 tcttttcgcg cagctgctcg atatcagata aatgcgtctg catgtatttc gtgatgctat
    13741 aatcattatc gtttttttct aaagaaagaa cgatttttgc tgtatcatca ttgaatggga
    13801 tgccttcata ctcttggctt gcttggattt ctagtggttc ttcttctaaa tgagaaaagt
    13861 ttttcgtgta cacataatca ttaccgaata aaatattgtc tttgttagat aaaaaatctt
    13921 ccaaggaact actaatttta tctggatcga tcacgttctt ttcagcatct gaactggata
    13981 aagatttcgt caaaacatca ccgtctaaaa acacaccact tgccagcaag ccgcttttac
    14041 cagccttgcg ataattcgct aatgaagtgc taagattggt ctgctccgcg ctaagataat
    14101 agccttgctt gttctcagac gaaatcttcc ccgtgtaagt gatatcgtcg ctagccagac
    14161 gttgttggat tgattcagtc tgattagagc gaatgacatt gttttcttgg tataccgcgt
    14221 catgataact gctgaataaa aacacattca accctaaaaa agcgacgaaa aatatccatt
    14281 cgattttttt gaaatccaat taattcgcct ccaatccagg taatttttcc agaaggtcgt
    14341 tagctgaata ccagttatct ttatatttca cataccattc aggtaagaga tcaacgacac
    14401 gattcgtttc tttgacattt tgccatgtat agccaacgat cactgaacca atcttgttgc
    14461 tatctgctcc cttagctttt aactgctgga tgatttcatt cgtacttgca agttctactt
    14521 cttcgtctga aggtatcggc acttgaatcg tattcaaact ggtttggata ttcacttgca
    14581 cagaaccatt cgtttcaccg ccaacttcca caccaatctg tcctctgctg tctgtcccaa
    14641 aaaccgggaa gccttccacg aatatccgat aatcgatcgt attgtcactg cggtcaaaat
    14701 atctgaggtt ccctaaagaa ctgcctaatt tgctaatata ggggaaactt tcagaaaata
    14761 cactagcatt ttcttttttc tctggcagct ctccacgaaa atcaactact tgcgtttccg
    14821 aatggataga cattgtttca ttcccaccgt aaaaaatcaa ttcattgctt tcatcattat
    14881 ttttcacgct atctggtttc tggaaaaaag catttctaaa aagtgtatag ggttgctgtg
    14941 acaggatata actatacttc ttcagtttga tcgaatgttt cgtagcatat tgcgtgtcga
    15001 tcgtcgatgg ttctgttgtc atttctgtcc attttgtgtc ttttgctgtt aattctttat
    15061 tgatatctgt ccaattcaac gaaatatttg cttcatatac gagatggttc gaataattca
    15121 aaaatcggat ttttttattt ccttgatcga attgtattct agtaaaatac atattttcgc
    15181 tttcttttgc caagttcgac acatctaaat ctaaatcaaa tgcttccttg tattctctta
    15241 gcaaaaaagg cgcattatag gccaactcaa aaccagagtt cagatttttc acctgttcaa
    15301 actgtgattt gtctcctgaa actacttcag aaagctttcc aaatcttgct ttatcgatga
    15361 tcaattgcaa tcggtacacg agattttcac tgttggtttc ttggatcatt cccgaatggt
    15421 gccatgtaac atgaagcgga agaaagactt cggaagtttt tcgattgctt tgagactcga
    15481 tcatttgagt gtttgtctca tctacgttga ttgaactttt tcctgctggg ctcagccaga
    15541 ttgcataaga gaagtatatg cttaacgcaa ccatcaaaat gagtcctata cgaacgactt
    15601 tttcagagat tctcattccc accattcctc ctcataaggt tcatatggca gcgagatata
    15661 aaaagtcgac ccttttcctt ccttgctctc tgcccaaata gcgcctcgat gcgccttaat
    15721 cacttctcta gaaatagcta atccaagtcc tgtcccacct tgtgctcttg cacgtgcttt
    15781 atctactcga tagaaccgct cgaacactcg gttcagatct tttttaggaa tccccaatcc
    15841 ttgatcggtg atacttaaaa tcacgttatt atgcgtttct aataagcgac aagtaatcgt
    15901 tcccccatct ggtgaatact tgatcgcgtt attcatgata ttatcgacta cttggatgat
    15961 tttatctgta tctacttcga cccaaagatc tctttgcgta aattcacgac ggatcttata
    16021 atttttttcc tgatttccca ccatcatatc aaaccggtca agaacaaaat tcactaactc
    16081 gttgaaattg acatattcta attgaagctg ggtattacct gtatccatgc gagatagatt
    16141 taagaggtca ttgatcattc gaatcattcg gtctgtttca tctagcgtta ccttcaaaaa
    16201 atttggtgct acttcctcgt ctttccacgc accttcgctc aatgcttcga tataactacg
    16261 catacttgtc aacggtgtac gaagttcatg ggaaacatta gagacgaatt cacgacgttc
    16321 ccgttctgtt ttttcttgtt ctgtcacatc atgcaagaca gctactaatc cgctgataaa
    16381 acctgattcg cgacggatca tagaaaagtc tactcgcaag atcatctgat cactttccaa
    16441 tgtactggtt gaacggtcaa gtaccatttc gtttggatct tctaataatt tccgcaacgt
    16501 atattcttct tcgatatcta aaagtgtcaa aatcgattgt ccgattgctt cctcgttttt
    16561 gatatctagc aaggaaacag ccatatcatt gatcgtaatg actttacccc tgcgatctgt
    16621 cgcaatgacg ccgtctgtca tatgggatag cacgctatcc aaacgattgc gttcagcttc
    16681 cattgtatct tgagcttctt caatacgttc agctaactga ttgaaggtat cagccagctg
    16741 tccaagttca tcttgtccat gcactttgac tttccgactg tagtctcctc tagcgattcg
    16801 aatggcttgt tctctcattt caccgatcgg ctgtgtaatc gaacgagcaa ctaaaacaga
    16861 tacgatcatt gaaataaatg ccgcaattag tgaggcagta aagaaaatga ctgctgtgtt
    16921 attgatttcc gaatattttt gttcgatatt acttttgacg taaagagccc cgataacggc
    16981 atcgccggtc ggggacagga tcggttgaac attgatattc acccgtttat cgtcgttatc
    17041 taaagcaaca taacgtttag tcgtgaaatc attgatatca acataatcat ttttctttcc
    17101 cacagcgctt tgttcattga catcggttgt tcctcggatg atcccttttt cgtcaacgac
    17161 ccgtatttcg atgatatcgc tagtcgcact gttttcaata aatcttttga tattcgcatt
    17221 tacctcttca tcatctgcat tatcttttcg attcaattcc gggctgattg ttgttgctaa
    17281 tgattccacc gtttgattca tatctttaat aaatgtattg atcgtggaac gttctaaacc
    17341 acgtataaag taggcaccaa taatttcgat cgagatcaat aaaatcagaa taaaggtaat
    17401 agcgatctta aaattgactg atcgaaaaaa gcggactttt cttttcatcc aattactcct
    17461 gttctgggtt acgcaaatag taaccgacac cacgacgtgt cactaaataa tttggatggc
    17521 ttggattatc ttcgatcttt tcacgcaaac gacgtacagt tacatccact gttcgtacat
    17581 ctccaaaata atcataaccc caaaccgttt gtaataaatg ttctcttgtc atcacttgtc
    17641 caaggtgctt cgccaagtaa aagagtagtt cgaattcacg atgtgttaac tcgattgttt
    17701 ctcctcgttt cgtcaccatg taagcttctg gatggatcgt caaatcacca atcgtcaatt
    17761 ccgcaggtgc tgcctcttct ggttccttcg cagccgtggc accgcgacga agatttgctt
    17821 tgacacgtgc aactaattct cggttggaaa atggcttggt cacgtaatca tcggcgccta
    17881 actctaagcc taggacttta tcaatttcag aatctttggc tgttaccata atgatcggca
    17941 tatcataagt tttgcgtacc tcacgagcaa cttctaagcc gtccatttta ggtagcatca
    18001 aatcaagtaa aatcaaatca ggttctacct cagcaacttt ttccaaggct tcttccccat
    18061 catatgcagt ataaacgtca tagccttctt tggctaaatt gaatttgacg atatccgaaa
    18121 tcggcttctc atcatcgaca actaaaactt ttttcataaa gagccacctc ttatatatta
    18181 ttgtcatacc ttctcattat accgtatttc tgcttttgtt tcatctttgc ctataaaaca
    18241 aaagagaaaa ttaaaatgaa agtgcaaaat actttattat tttttgttct aacttcaata
    18301 aaaaaggaaa acactgattt tcacctcagt gtttcctttt tttgatttat ccttctaaaa
    18361 cttgtacacc gacacccaat aatcccggac cagtgtggac tactaatgcc ggagaaatct
    18421 ggccaaagta gatttggttt gcttgtggga actctctttt aagtctcgct tccatttcaa
    18481 ttgcttcttc tttcgcatcc ccatgggcaa cagctaagtt gaagaccttt gcattaccga
    18541 ttttttcttt gacgtaagca accgttttat ccaaactttt tttacgtccc ctagcttttc
    18601 ctactgtgta ataaatccct tcttcattac atgagatgat tgggttaagc tttaaagcgg
    18661 ttcctacaat agaagcaact aaaccgatcc gtccaccttt ttgaagatat tccaatgtcg
    18721 caacattaaa gaaaactttt gttctttcga ctaactcatt cagattactg atcagttgat
    18781 cccattctac tccttcttcc agccattttg ctgcttggat cgcagaaaaa cctgcaccaa
    18841 taccaatatt tttcgtatct agtacatatg cttccaattc ttcttgctgt tgtgcaatta
    18901 aacgaaccac attatatgtc ccgctcaatc cactggaaat tgttaccgca agtacctttt
    18961 cataaccgtc ttttttgatt tgctcaaaga tttctgtgat cgactcacca tccggcaaag
    19021 atgtaccagg gatttcctct ggcaaacgtt gatagatctc ttcaggggta atgtcaattt
    19081 tatctgtata tacctgatct ttataaataa ttttcaatgg gatcatatac atctcgtatt
    19141 tttttatcaa ttcttctgga acatccgtac ctgaatctac taaaattgcg attttattgt
    19201 ttttcatttg ttttctcctc ttttaaatat tcggtttcta gaacaatcgt tttttccgca
    19261 agaagtttca ttgcaaacga taaagttgcc gctcttacag caatatattc tacaggggtt
    19321 gcctggtcga atttttgcgg atgagattta ccttgtgcta attggctgac catccacaca
    19381 gctgcttctt gttcatcgca aaatagatta taggcttcac gaattcctac tgtttttcct
    19441 tggaacaata tcccttcttt gatttcagga atagtcaata cttgtttcaa cgtagtgatt
    19501 gcaagtaaaa atgctacgtg ttctttgtta tatctttttt taactggtgc tggtatcaaa
    19561 cctagtttaa cgtaattgtt taccatagaa gaggtaagca atggatgctt ttctccttgt
    19621 atcacaggtg aaagataacg atcaactaac gttctgactt ggtccatata aagttctaag
    19681 tcagggagtt cttcccagtg aggcagacgg aatgtttcta gttccttccc ccactccatt
    19741 aattgctgac gcattttttc catcataacc gctccttttt tcatttcata aacatgatat
    19801 cagacttatg ataaacattc aatctagttg ctttaactag gtgatttgat tagcgggatt
    19861 ccatgaaaac aaaaaacaag aaggctaagc cttcttgtct tttatcgtta agaacaggag
    19921 acccgccggg gagacggcgg gaaaggagta aaaaatgaaa aaagtgttag ggtttgttct
    19981 cttgcaagaa ctttgtttgt tggtatgctt atataatagc gagcaaatgt gaataatcta
    20041 tgaatgattt cctataagga agtaaagatt tttctgattt tctttaaatg tagtcttgca
    20101 ttttaaaatt cttagtgcta ctattattaa gtactttttg tacgggccgt tagctcagtt
    20161 ggtagagcag ctgactctta accaaatgct ctatttgtga aaaaagtttt ataaagcttt
    20221 ataaataggt ttttagtttg tgaatttttt cattgccaac atactgccaa caaattgcca
    20281 acaaaaaaat tctaaaaaca agaagcgata ccccaaagat attgtttttt gtttttttat
    20341 tctttaaacc caaatgcgtt ttctaatgct tctgctgcac gatcttttac tcctgcggtt
    20401 aaatgcgtgt ataaattagc agttgtcgaa tacgatgcat gtcccagcca ttcttgaatg
    20461 gatttcatat tctggccact atctaacatt attgaagcat tagaatgacg caagtcatga
    20521 aagcgaattt ttttcaatcc atttttctta atcaacttac tcaaacgatg cgttacatat
    20581 tgaggcttca tcaaccgtcc atcttccata gtaaatacgt actcattatc ttctaaaata
    20641 taatcatctc ctaaaagaat acgattatct tgttgccatt tagcttgatt tactaatgct
    20701 cgttcaatcg tttcaaccaa aggtaatgta cgatagctgg aagagttctt tgtttgatct
    20761 ttctttatta actgtgacat attctctgga ttttctatga ttacatggtt tatcatgatt
    20821 tttttgttta aaaaatcaat agcagaccat tttaatccta aaacttcact tcttcgcaat
    20881 ccataaaatg ctgttaaaag aactaccaaa tagagttttt cttctgacaa aatgtcaagt
    20941 aatttcaaga tctcttgttg attataaact tgagaaacaa attgtttctt ttttggcttt
    21001 cttactttat ctgctacatt tgtacttatc aattctgtaa taacagcctc ttgaagagct
    21061 ttgcgtataa atgcgtgata atgaataacc gtatttgctg tacagttatc atttaacata
    21121 tattgataaa actcttgtaa atgataaggc tttaacctat ttagtttaat ttttttagga
    21181 taaaaaaact tgtaaattcg attatggacg taatgcttat acccaacata tgtgttaaat
    21241 gctacttgtt tttttgatac ttccaaataa ttaagcaaat agtcataaaa caataaattt
    21301 ggattagcac attcacattc catagctaat tgactttcca attctagtaa aaattcatct
    21361 ctaatttttt ctgctcgttt tttatttcct ttctttttta attttgtatc tttccacttt
    21421 tgtctaattt ttccgttaga gtctttataa gatacaacta cttgtaatgt tcctcttttt
    21481 tcaattatgt ttgctcttaa tttcaattca atctctcctc ttaaactgaa atatcggcaa
    21541 aaattttgac tattttatta tagcatgatt tttaccttca tcttgtattt tcaatatata
    21601 ctcaatgaca gcttttttag gtattttgta ccttgctcca atttttatat tttctattga
    21661 tctatctttc agcaaattat aagctttatt ttttccaata tttaacattt tagttaaatc
    21721 agtaattttc aatatcgatg gataactttc aaatattttt tcataatctt gaaaatccat
    21781 aaaattcact ctcaattctg ataataagta ttgcactttt ttattttagg tgttagaata
    21841 acaaagtatt aatttgggct gttagctcag ttggtagagc agatgactct taatcatcgg
    21901 gtcgcgggtt cgagcccctc acagcccatt gggtgccaaa cccttaaata cagtgtaaac
    21961 gttggtgctt agcatttaga aaacgtatat actgtatttt tttgttttca aaagagactg
    22021 tagtacccct ttttttcact aaaagcccac tgtagtcatt gatatttttg ataagaaagg
    22081 aacataatta tgcgtaatga aattgctaat ataacaataa tccgagaaac tttacaaaac
    22141 catactgcaa atgagatctc taaacataca ggattaaatt tgagtactat aaaaaaacta
    22201 aaaagtggag aacgttcaat agaaaaacta aatttacatg atgctatttg cctcactgaa
    22261 tttggtctta aaaataatag aaaaaatgtt gaaatcaaca tatggaaata attcttaatc
    22321 agctgctttt gtcaaaagca cataggaatt tcacctctct ccaagtttac ggcgagcctt
    22381 acggaagtat cattatccca acttcaaatc atcgcttttt attgactata aaaattattt
    22441 tagaatgttt atcgctcctt ctttacagta agaaatcatg gcgcaactaa aatatgctcc
    22501 ttgaagtgtt aacgtactga tagaatgtat ttaaatttca atgatcaata ggttttctgt
    22561 ttaaaagaat ctactaatcc tttaactatt agaagattct tttaaacaga ataagtggaa
    22621 aggggacttt ttagttagtt aattttaaat tttaagatcg cttctatcaa tttaatttct
    22681 agctgtctta gcatatactc atctacatag gtaataaatt gccccgcctc attaaatcca
    22741 tttgtcaaac ataacttagc tatataacta tgataatgtt tgagtatcat cttgattgaa
    22801 attggatctt cttctagagc agattggata atggatagag gaactaatgg atactgccgt
    22861 tttttggggt tgtctaccat aagtcatacc ccatttttaa ttgttcaact gccttctttc
    22921 gttgatacca gataccactt gtactcatat tgaggatttt accgatttca gtatcattaa
    22981 aacctgcaaa ataataaagt aggataacct ttcttcttat ttccgataaa tgatcaatgg
    23041 ctgccgataa cttttgatct gaaattaata tttccattcc aaataagaaa tagacatctt
    23101 gttcatcaaa atgagtttgg gagtacttac cttcatttga attcgtcaat ggttcctcat
    23161 tatctgttat gttaatgttt cgttttcgaa tagacttagt ttcgtttcgt aatacttttt
    23221 tacagtaggc atcaaattga tgttgtatag atttctcatt gtaattttgt tgcatgataa
    23281 atatgctcct tttttcccct ttccactaat acaacaatca gaaagcgcct ttttttcgaa
    23341 aaaactagaa aaaaatatcc ttctttaaac agaaaatctt tttataaaaa aaaggagttg
    23401 gcaattaagc agccactcct tttttatatt ccttataaaa ggtaaattat tttttataac
    23461 tgaaatttgg tattgatgac catcgtttct ttagatagtg agcaatcatt ttaggctttc
    23521 tatctctagt aaagattcct ttcttatttc cttgcactct taaaatccct tgacttgttt
    23581 caaaatctgc aaaattccac acttgttcac caacaaaatt gtcacattta tctattactt
    23641 cgtgatttgc ttcgtaataa cccacttgat actcttctgt aaacattgtt ggtactgtat
    23701 catgaagtcc agctactgta tctgctccat attctgtaaa cattattggc gtatcgggac
    23761 atctttccat ccatccatct agctcttttt tcaacagata ttttgcatgt tttaggtctc
    23821 cacctgaaac ataccaacca taatagcgat ttaaacataa aacatcgaca atatctccta
    23881 ctttacaaat atctggtgtt gactttaagt aagtaacgat tgttacaggt ctcttctgag
    23941 ggtcaagttc tttagtcaat tcaactaaag gttcaaaata ttctttagcg ccttcagaat
    24001 ccgaatctgg ttcatttgca atagaccaca ttacgacact cggatggttc ttgtcgcgtt
    24061 ctatcaattc cttaatcgcc attttgtgag cattttctgt ccgtatttct ttccaagtat
    24121 catgtttttc tacgttgtca tctaataaag tagccatgaa attcaaatgt aatcctactg
    24181 caggtacttc atcaataact acaatacctt ctcgatctgc taagcgcata atttcttctg
    24241 aataaggata atgtgaagtc ctaaatgaat tagcaccgat ccatttcatt aaattaaaat
    24301 ctttgatatt tactgcttca ttcaaaccac gaccattaat atacgtgtcc tcatgcttcc
    24361 caaatccctt aaagtaaaaa ggtttattat taatcaaaaa cttcccattc gaaacctgaa
    24421 tactacgaat gccaaattct tctgtgtacg tatcaatcac tttattttcg ttaattaaac
    24481 taactttcat actatataag taagcattta gaggctccca aagtgttggg ttcttaagtt
    24541 gaatcactcc ttctttcccc ttagcttctg ctataagttg agcttcttcg tcatagattg
    24601 ctattagaat ttctccggtc cattctgtat cgatattaaa ttctacattt gcatctgaat
    24661 caattatttt tgtattaata gtgacatcct ttatatagtc tttcggagtt gtatacaatt
    24721 taacgggtct attaatgcct gcataattga aaaagtcaaa attaaccaaa ttttcttttt
    24781 ttatgtctcc atcttcctgt atcgtttctt tcaaaatccc tacaggtaaa gtgctatcgt
    24841 caataacatt gtttacagcc actgtaattc tgttacttcc atcttttata aactgattaa
    24901 tttctgattc aaatggcgta aaccctcctt tgtgttgatt aatcaatttt ccattcaaaa
    24961 atacttttgc ttcatgtgtt gcagatccaa aacgaagaac taatctttct tctaataatt
    25021 ttttaggaag aatcacttct ttttcatacc acacccatcc aacatgattt ctaatgttct
    25081 ctgtttcaaa taaatcatta taagaagaag gaactgaaat tggataactc tctttttcaa
    25141 ccattggttc taaagcaagt tcttcttgta agtcatcatt tgtatctaac ttaaatctcc
    25201 aaagaccgct taaatcaatt acttgcctcg tctctgtcat aatgggatat aacatgtaaa
    25261 tcactcctat ataatttaaa tattaaataa atgtatcctt taacccacaa taagcatcta
    25321 ttggacttat tcccttaaat tcagaaaggc ccattagttt gtccactaat atttctaaag
    25381 tttcttgttt tccatcataa gtattgatat aagtttttac ttgaggcaca tcagctaaat
    25441 gaaacggaca ctgtacagat acaaaaattg ttggaatttc atgaatataa aatggaatat
    25501 ccggagtacc tttacttgct ggccaaacaa ttctttgaac tgtacctggg ttaacattag
    25561 ctatattaat aatcaagtca tatttttcag ttaaatcttt gattggtctc ttagcagcat
    25621 atattccagc tatattttta attttttctt cttgcgataa tcctttcaat tcttcttcag
    25681 gtgaaatgta tttttcaact tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa
    25741 tggcctgagg tctatttcca gcaactactt caccaaaccc accttttacc ccatgtacat
    25801 ctaccagtaa aatcctttta tatttttctg gtacaatagg ccaaatatct tgtttatctt
    25861 taactagtgt aattgcttga tcagctacct ctttaaaaat tgatttattt tcaattaaac
    25921 caattttttc catagcttct tgtttcggta acaaaatttt attcttttct tttttatgca
    25981 aacctagttt agcctttaag cctaaaattc ttcttaatgc atcacgtaaa cgctcttctg
    26041 aaataattcc tttgtaataa ccttccatca tataattaaa gtcttcatct ggatcattaa
    26101 aaaataggaa tagatcacat ccagccgcaa tagaagtggg caataattct gaccgtttca
    26161 ttgccccagt cattcctaac atatgactag catcagtaac tactaatcca ttaaacccga
    26221 gttttcctct aagtaagtca gtgattaatt cttttgataa agttgcagga agtaaatctt
    26281 catctttcag atcaagatta aaatgttttg aataagctgg catatggata tgacctgcca
    26341 taattgaagg taatccagct tctattaagc ctgagtatac ttttccaaaa gaattatccc
    26401 attcttctgt acttaaacta ttaatactaa aagatagatg ttgatctcgt tcgtcaatac
    26461 cgtcaccagg gaaatgttta gctgcaggca aaattccact ctcttgaata cctttcatat
    26521 aagctaaact aagttctaaa actttatcag ggctttgtcc aaaagatcga ctagaaatga
    26581 taggatttct ccaatttcta ttaatatcta caataggagc aaaactccag ttacatccaa
    26641 tagccgatgc ttcaattcca gaaactctcc ctaattcata agcatatttc gcatcatttg
    26701 ttgcaccaat ttttacttct acaccaactt cggtaccatc tgtacaagct ccgttaccac
    26761 ctgcttctgt attagcagca attaataaag gaattttgct ttctgtttgt aggatatagt
    26821 tttgatcata tacctcatca gcagttcctg gattatatcg tacagctcca atatggtact
    26881 gattgaccat actacttaag taatttttat cacgactaga tcccatatta acaaataatt
    26941 gacctatttt ttcttctaaa gtcatagcat caatcgtctc ttcaatccaa gaaatagcat
    27001 cttcatctaa atgatatggt ttttttcgta agtctacttt tatagtcatt ttctttctcc
    27061 tctacttatt agtaagattt ttccaaaaag tatctatata tttttgttca atatctatat
    27121 actctcccga taaacattta aataaaatat cagttcttaa gttttcccat gattcttttt
    27181 ctttattaac caaaatagga taaaagaaaa ctttattctt atttgcagct tctagatcac
    27241 caggagaatc accaatcatt aatatcttat ccaaatcaca accattttca attacacttc
    27301 gaataatgtc ttcttttttt cctttatctt gacaacacag ttcatcaact aaggataata
    27361 attcatgtct ttcccattct tcttgaatag cttccttatt agctgagctt acaataatta
    27421 tcttcccata ttttttcaga gcttttatag tatctctagc acttaaaaag ggtttatcta
    27481 atcctttagc ttgactaatc tgtcgattga cttcttccga ccattttaaa gcttttaata
    27541 aatcctctga ctgagagtat tctatttccc tcttcaaaga tggattagac aattcttttg
    27601 ttacagatac ccagtgcttt aatcgattaa tcgatggtac atcatttttt tctaatgttt
    27661 taactaaacc taaaaatctg tttattccac gagtctctga atataaatta atattattcc
    27721 aatcctgtag gaatttttcc ctatgatgaa catcaaaaat ctctgcagca attggcccga
    27781 aaaagagatt gtgtttataa gtcattgtat ccatgacaca accatctgaa tcaatacaaa
    27841 atatccaact gttcatgttt tttctcctag ttatgttttt gatttagcaa attataattt
    27901 tctaatactt tttctttaga taatggatat acgaaaaata aaatcaatcc tacaacaaag
    27961 aaacaaactg ctggaatcaa cgttgaagag ttatacagct tttgtaaaac ttctccactt
    28021 tgtacttttg ctaacgaatc ataccctatt atagaaagag tccaacctcc gacaccgcca
    28081 gctaaagctt gtccaatttt tcgggcaaaa gaatatactg cataaatagt tccatcttct
    28141 ctttggccag atttcacttc ttgatcatcg attacatcag taatattcgc ccaaatgact
    28201 gtattaaaaa tacctaaccc catatatcca acagaagtaa gaactaagaa tacatacata
    28261 ttactaggct ttatcaaaaa taaaagtaaa tatacaatag cagagaagaa catccctact
    28321 gacgccaatt cttttttacc gtatttttta ctgaaaatta aacttacagg tgaaacaagt
    28381 accaacatca aaataggatt gataaagttc attaaaacta ttccctgtgc attattataa
    28441 taatctggaa atacataatt gttcatactc attgttaaaa gctgagcaag caacaaaaaa
    28501 atagctgcag ctattataga aagcaacgct ctatttttag ctaatccttt taaagatttc
    28561 aacaatgaaa cttttaactg cgattcatca cttttaaccc gttctacagt taatttataa
    28621 catattacgt aacaaataac tgcacacact gaaaatactc ctgccacaat tgtaaaagtt
    28681 gatccaccac gtacaatttg agctccagaa ctatctcttg tataaataaa aattggtgtt
    28741 aacacgccaa tgaacatacc tgctaaggta gctcctatac tacgaaatgt tgataacgct
    28801 gtcctttctt ctgcaacaga agtgatagct gatgccattg aaccgtaagg aatattaata
    28861 gatgtataaa aaatacttcc ccatagtata tatgttacat acatatatat aactttaata
    28921 ctcatagaag ctgatgccat tgatgtttga tacattaaaa aacttgctaa agcgactgga
    28981 ccactcatcc gtaaaatcca cggtttaaat ttgccagttt tagatggttt tgatccatcc
    29041 acaatacgcc ccattgttat gtctgtaact gcatcaataa atcttgctac taaaaataat
    29101 gttcctacgg tagctccgct tatccccaat acttttgtat aaaaaatcat taaaaaagaa
    29161 ctagcaaaaa tgaaagtgaa atcattccca aaatctccaa acatatatcc tattttgtct
    29221 cttagaccaa attttctttc catataattt gcgctccttc ctattgttaa gcgttttcat
    29281 tctgttgaac tgccaactta cgtactatac tctgaagtat aaagagttag tatttatttt
    29341 ttacatttta tagtattatt tattttttct cctaataatt ctttatctca taaaacttat
    29401 ctatatattt actagctatt cgagttactt cttcaaaatc acctttttca acaggagcca
    29461 acaaattccc acctacacca actgcaacaa cgcctgcttc aaaccactct tctaagttat
    29521 ctagacttac tccgccagta ggcattatat ttacatatgg taatggagct tgatacgttt
    29581 ttacaacaga tggaccaaaa gcagaacctg gaaatagttt aatgatttct actccagcgg
    29641 taagtgcctc ctttatctca gtaattgtca tacaacctgg taaataagga atttgatata
    29701 aattacaaat ctctgctgtt tgtcgatcaa aagatggact tacgatatat tctgctccag
    29761 ccataattgc aatacgagca gtaaacgcat ctaaaactgt accggctcca attattacat
    29821 ctttttgatc agcatatttt ttagcaattt ctttgattat ttcttcagca ttcggaactg
    29881 taaaagttag ttcaatccct ttaattccac ccgcagtaat tgcatttatt gctttaatgg
    29941 cctcatcttt attttttcca cgtacaaccg caatgactcc agccgcttct aacttactta
    30001 aaatttctac ttttttcata tctatctcct tcttttatgg atacatactc tagctagttg
    30061 tactaactta caataaataa atttaagctt ttattttttg tattattttc tactttcgat
    30121 ttcaaaaaaa ttaaaataat tatacgcatt acgataacaa atattctcca ctatctgact
    30181 aagatacatt tcatcatctg gaattcgacc tgctttaacc cactcgctta gtaattgaca
    30241 taatactcga cggaaatact catggcgagg atagcttaga aaacttcgag aatcagtcaa
    30301 catacctgta aaattcgaca acaaactttg ttgcgccatc gtagtcaact gttgacgcat
    30361 tccttcagct gtatcattga accaccaagc acatcccaat tggatttttt gaatcatacc
    30421 cccttggaag ctttgcattg ctgttgctag tggaagccaa tcggttggat ttaatgaata
    30481 taaaattgtt tttggtaagc acccactctt ttcagcagtg tcgaacaatg ctacaacatt
    30541 ttgtgtcaaa ctagcttgat cccccatcga atcaccaccg acatctactc caatttgttc
    30601 ctttaatata cttgaattat ttcttaaagc atttaaatgc atttgcatcg tccaaccata
    30661 tcttgtatac tcagacatca aatgtaattg aatacctgta acaaattgtg caatttctga
    30721 taaatggaaa tccgtttttc ctgctaaagc ttttattaac agattgtcga gtattttttc
    30781 atctgctatt tcataaaaat aagtattcaa accgtgatct gctaatcgac agccaatttg
    30841 atgaaaataa tccactcgtt ggctcaatgc ctgcttcaac ccattaaagt ttttgattgg
    30901 tatacccgct gcttgactaa gttttaaaat atagttcgaa aatcctgatg atttgatact
    30961 ccagacctta tctggacgga aagtaggtaa taccttgaaa gaattatcct gtttagaaag
    31021 ctcagtatga tatcgcaagt catctgtagg atcatccgtt gtacaaatgg ctttcacatc
    31081 catcatcttc agcaattctt ttgggcgata ggaatccgtt tggatttttt cgttggcacg
    31141 tcgccaaatt tctggtgcat tcttctcaga aattgtcaaa tcaatatcaa agtaacgttg
    31201 taattccaaa tgagaccatt caaaaatagg attacctggc gccttctcca ctgtttttac
    31261 aaaagctaaa aatttttcat aatcattacc tgcaccactg atcaactctt cttctatccc
    31321 attagctcgc atcaatcgcc atttataatg atctccagct cctttatcat ttaaccatac
    31381 ctgagtcaaa ttcttgtatt gcttattttc tagcaaacat gctgggtcta aatgacaatg
    31441 ataatcgatg attggttggt gttctgcaaa tttatgaaaa agtcttttag ctatttcatt
    31501 ctctaataaa aagttcttat ctaaaaacat atttaagcac tcctttcctt cccttagttt
    31561 ttagaagaga aaatttttct tctaaataga ctccattata taattattta ctcgccccct
    31621 tttcaagcgc ttcccaaata ccatttaaat aaactgcacc tagagctcgg tcatataacc
    31681 cataaccagg tctaccggtt tctccccaaa tcatccgacc atgatctgga cgaatatagc
    31741 cattaaatcc attatcgtac attgttttta atatcttata catatctaga gaaccatatt
    31801 ctgatttatg cggcgcttca taaaaatcga gtccattatc acttaaatat ttgatattac
    31861 gaacatgtgc aaaaggaatc cgatcccgtt gacaaaattc agatagaatt tcatagacat
    31921 cattatctgg tcgttctgca atacttccag tacataacgt aataccatta gaaggagaat
    31981 ccactacatt acacaaccat tctaaatcgt cacgattttt aacaatacga ggtaatccga
    32041 aaatagaata tggtggatca tctggatgaa ctgccatttt aatcccaact tcttcacatg
    32101 tcgggataat tgctttgaga aaatatgcaa agttctctcg caatttcgct tcatcaactt
    32161 ctttataggc ttctaataat tgcttgattt gtgataaccg atgaggttcc caacccggca
    32221 attcaaaatt attagtagaa ttttctactt ttctaataat ttcctctgga tcagtaggaa
    32281 tattttttga aatataggct aacgtagaag acccatcatc taaatgataa tccaaatctg
    32341 attttaccca atcaaagact ggcataaaat tataacagat aacctcaatc ccaaattctg
    32401 ccaaattgcg aatcgtctgt ttataattct cgatgtattg atctcgtgtt ggcaaaccaa
    32461 ttttgatatc atcatgaaca ttaacactct caataacttt caaggtaagg ccattggctt
    32521 cgacttcatc ttttaaagct ttaattcgtt ctctcggcca gacctctccg gctggtatat
    32581 ccattaatgt tccaacaatt cctttcattg ctgggatctg acgaatttct tgtagtgaga
    32641 ttttttcttc atttgcccca taccatctaa aagtcatttc catagttact ttcctccaac
    32701 ttctgttata tatttattga ttgttttacg aactgcttga ggacctacta gcatttcgca
    32761 aaagattttt tcaattttct ttcctatctc aacttgatat aaatctaaac caaatatttc
    32821 cttattttct aagatttcct gtaccgcaca atgaatcttt gcaggatctt gttcacctaa
    32881 tttcaatact gagaattttt cctgcaactc taccaataaa ggatcaggac ttggcacaaa
    32941 attttttcct tgatcatcga ctgccaataa ataacgtaac caagcagcaa tagcgatagg
    33001 aataaatgtt agattttctg catttccgtc tttttcgaca tactttttaa ttgtttcacc
    33061 atagcgaatc gctacttttt gagaagtatc tgttgcaatt cgttgcggtg tatctggaat
    33121 atagggatta ggcaaacgtt tattgattac ttcatcaata aattgttttg gatcaataac
    33181 gcctggactt tcaactactg gtaagccttc tacataacct atccctttaa ttaagccaac
    33241 caaatcatca tcttgcattt ctgcctgaat tgatttatac cccaacaaac aacctgttat
    33301 tgctaatgcc gtatggagcg gatttaaaca tgtcgtaacc ttcatgatat ctgctttatc
    33361 aactgtttct ctatctgtca taatcacacc agccgcttcc agtttaggtc gaccatttgg
    33421 aaaactatct tcaatgacta gataatggac tttttcagta ttggtaaaag cagcgatatt
    33481 ggtatttttc gcagtatgaa ttatatctaa gtcttcgaaa cccgccttca ataaatccat
    33541 tttgacactt tccgaaggat ttggcgtaat tctatcaatc atactccaag gaaaagtcac
    33601 tttttcttta tcctttaaat aagcaataaa tttttcagaa acgtatcctt ttttttgcca
    33661 agcttctgca atcataaaaa tgctatcttg aaaacgttga ccattctgtg agaaattatc
    33721 agtagaaacc atcgcaatcg gtaactctcc agcattaaac ctgctataca acaaggatgt
    33781 cacaatactc atcgtatgtt gaggattggt aggtccagat tttatatctg cttccactac
    33841 cgaaaaaaat tgtcctttag aatcttttaa actgtatcct ttttcagtaa tagtaaacgt
    33901 tgtaaactgt aatgtaggat cttcaaaata tttaaccact ctttgatagt ccgttatatt
    33961 ctttggattg caatataaac tatcagctgt tactgctaaa agtctttttt tgagttcacc
    34021 atcttcaccc ataacaatct ccaaaatatc attgttgtag tcatgataag ctttttctac
    34081 tacttcttca tcaaatgtct cacacacaac tactccggat tctaattcat tttgatctgc
    34141 taagcattga gcaatttctg catgaaatcc tcgataaaga tttcctccac caaaatgaag
    34201 ccacactgga tgtttctgac cttcttgttt gaccgcttcg acattaaaat acggtagttg
    34261 aacttgcatt ttgttcaatc cttcgataga atcttgccag tctttaatca aaaccatatt
    34321 tacccctcca tactgatatg ttagtttccg ttacaattgt agtataaaat cgaatgtaat
    34381 cgctgtcaat aagaaaaaac taaaaatatt cttttttcta ccatgtcaga attattgtgt
    34441 ctttttttag taaaagcaca ccaatatata ttttttagaa ctaacttaaa ttaaagctta
    34501 atttctcttt gatcttattt gagtattttt tgttttttta atagctagta tgttactttg
    34561 aaatttgata ttatataacg agcatagaac taaaatatca aattcaaaaa tgaaattttg
    34621 agagggatta aatatgacaa ttaaaactag tctccaaaat caagcttatc aatctattcg
    34681 tcagcaaatt atctacgctg acttagaacc tggaaaaaaa gtatcagaaa aaaaattgga
    34741 agaaacgctt tctatcggtc gtactccaat acgtgaagca ttgattcaac tccgtcatca
    34801 aggacttgtt gatacaattc ctcaaagtgg aaattatatc tcattaatcg acttagattt
    34861 agcaaaaaat gctcgttttg tcagagaaca tttagaacga caaattatgc tagagtgttg
    34921 tgccaagatg aataatcaaa agaaacaagt tttagaaact ataattgcag aacaagaaaa
    34981 agctgttagt caaaagaatg ctcgtagttt ttttcataat gataatttat ttcataaagc
    35041 ttgttttgaa attgcagaac gggaagaagt atggcaatgg ttggaagata ataatacgca
    35101 cttggaacga tttcgttggt tacgagtaaa aacggaagga ttaaaatggg aaaaaattat
    35161 ggaacaacat catcaactat ttaaagcctt gaccatgcaa gaccctgaag aagctaactt
    35221 cttaacatcc ttgcatcttc atttaatgct tgatgaacaa aatgttgtat tgactactca
    35281 cccaaaatat tttaaaaata tataactgct aaatttcttt tttttaattt atttacattt
    35341 ctgattacta cagaaaacta actacttttt ttatgtaaag ttctgtcaaa tatagtcact
    35401 ttatattact ttctcttgag taaaaaaaca tgtgaggtcg taaacaaaga ttgaactatc
    35461 tacattcata ctctataatc tgtttatttg cagactgata atagcaaaag aaaacttata
    35521 atcaaataat atcaacattt gatcacataa tttgagattg aatgctgatt tacctatgtt
    35581 ttacgtaatg gtctttagtt atttttgctc ataaaatgca taaaacacgc gtttttatgt
    35641 gtgcttgaaa tttcttggct ctataatttc taagactgat aaaaatattc tatcggttct
    35701 ttttgcaaat aaaaaaacat atagaagctc cattagattg aatcaccaca acacaatcaa
    35761 aggagcattc cgtatgcttg attttcattc tataaaacta cttttaggaa tcaaagaaaa
    35821 tcacaacggt tcaaactaaa agcaagaata tccgaagtac cattgtttca tcgatcatga
    35881 gaaaagcatt cacctctgtc tgcagatgtt cttatatcgc tctgtcagtc ctattaataa
    35941 agtactgttt tgttttcaaa aaaactatta cgagtacttt ttaagagaat gccttgcaaa
    36001 tattcatcac acaccgttat ataagtcaaa tagtttcttt cagtcgtcaa gaaaagattc
    36061 tcctgtcctt ggcattatca gttaagtagc aacttaattc tcggacttgt tctactctag
    36121 caaaatttat tttcatcaac ttacgtccaa atgttttttt gaataattac ttcatcgcgg
    36181 gtctggaaga cttgcctcat tcgataagtc actagctttt ggcgtccttc aggatttcta
    36241 gtatacacca actgacattt gggcagcgac gattgattta attcgtttag aaaaaggtta
    36301 gcgatttgct tatttcggcc aaagcgttct ggtaccgtca gaactatctt tttcccttcg
    36361 attaaatacc gcggattctc cagcggtgct aaaagctccc tcatagcttc agtaaaaagc
    36421 tgtccttctc gatagccagc attctcaaga tagcatctaa attcctgctg atcagccgct
    36481 acgattactt ttctatcact aacttcacca aggcgctgca acgtcgtttg aacgcatatg
    36541 gccaagcttt ttttgcgggc acattccttt tcccatagct tttgtcgcct acgaatttgg
    36601 aaatattcca gcaagttacc tttaaatata gctgaccaga agcttagagg ctctatattt
    36661 tttactacta tcggcaatga agtttcttgt ttttgcttta cctgatcgac tttcctaacg
    36721 acgattggca tagataggtg ccagccatca gctaaagcag tactccattg ttctttcaac
    36781 cgacttcgtt cacaagaaaa atagaacatc gactgagtat aagtttccat cgtggtggtc
    36841 gtatattcca gtggtaaagc aaagcgatcg ccaccactct caatttttgg tgaagattca
    36901 aaggtcagac ctgaaatatt gcccaaacgc cgtagcattc ttttcaaatc aggctgttca
    36961 gacagaggaa ctcccggtaa tccaatcgct aaatgccaaa tgttcgttac tttattttgt
    37021 gcacctaggc gtaagccccg ccctcgtagt tgttgtgttt gcacaaaagt ggaagcttga
    37081 ttagccaaga tcaacagatt aattgcagga caatcccagc cttctcctag caaactaacg
    37141 gttcctacca aaatattcaa cttcttttcg gttaataggc gagtaactgc ttgcatcaat
    37201 gcttggcggt ttttgtcatc caccgtcaaa taaaaataac ccgacatata aggatgttca
    37261 ttcatacccg tcgctagttc tgccagctca acagttacta gcattttcgg taacagtaag
    37321 aactctccac agatggcacc gatttgctga gtgttaccta atagacgaac aacttcatca
    37381 aacaaaggaa ccacgccata agctaaggta ccatcactgc ctgctaatac ctccttgcaa
    37441 atacgatcca tcaaaatgac cgctgtcagt tcttgcttca gctgaacttc ttcttttaac
    37501 aaaatttgac aaactgcttg ctttttctca ggaatcgcct gactaccaac agtgtctgga
    37561 tactctggaa acaaatgcag cgactcatca aacaatcatc cagctttttt caattgataa
    37621 aaaataggca attccggttg atcttcaaac atctgctgat aaatagtgat cagctctttt
    37681 ttacctaaag atggaactga agctttttta ccttgctgct tagaactgcc taatagctgc
    37741 caatgttcgg gtatcaattc atagttatga tgattcaagt aaattaatgc actagtgaac
    37801 agctcaaaat tttcataaat ttccttcgaa tgctcaagcg gatgctgaac aaattctaaa
    37861 gaaagcaaat aatccaccgt ctcttggctt tccattaacg cttccaccag ctcattttgt
    37921 ttctctaaaa aggtaaaata atgctgagta gtttcctgat tagccggaat caaatataga
    37981 aaatcctgat gaggacatag aacctcctcc tttaccaact cgggaatgct aatttcttcg
    38041 tcaatcaggc cattttaact caatatattg cttccactcc gtctgagagc tatcaatcgg
    38101 aggcgtggct gtcaaggaaa tcgtcaacag attcggttgt tttttcttca agtcaatcaa
    38161 caattcaccc cagctttttt ttaaatgatg agcttcatca aaaaactaac agctccagct
    38221 gatcaccata agttggctcc tccttcagtc ggttgtaaat ggtctgatac gtttccactg
    38281 taatatgact aggatgttca atcgccccag aaagatagca actatctgct ggatttacta
    38341 agaaatattc ctcgattgtt gttaaccact gtttcttcaa tattagggaa ggaaccaaaa
    38401 ttaaggtttt acaatccaaa cgtcggatca tctcaattcc taaaaaggtc ttgccagccc
    38461 ctggtggagc cactagatgg actgaattgt tatttaaata agtagcaaag ttagtcaata
    38521 tacgcttctg atagctacgc cactgaattt tttcatcaga tagccacatc ccaggtcccc
    38581 ctctcacgta ttttctctat cataatctaa atgaatacag caaaaaaaag atttcttcaa
    38641 gaatatgtgt ttttatagta atattttact tactctaaac tcaaatgaaa aggtaaaaaa
    38701 actggtagta caatctttta aattctcatt gttttgtacg ttctttacta tcaaaaaaac
    38761 aaaaaaaacg cgtttctacg cgtgaccaaa atttttagct ctataatttc taagactgat
    38821 agaaaatcat tctgtttttg aagtcttttt tcataaatac tacttgattt attgtgtata
    38881 ttgtataaaa gattgttcat aattattttt tataattaaa atttggtatt gatgaccagc
    38941 gattccgtat ataatgcgca atcaaattaa gctttctgtc tctggtaaat attcctgttc
    39001 tgttttcttg tactcgtaag atttcttcac ttatcgcaaa attcataaaa ttccacactt
    39061 tttcataaac aaaatgtaca catttatcta ttacttcata atagtctact tgatattctt
    39121 ctgtaaacat ggttgctact gtatctgcac catatttgat aaacatttgg gctactccct
    39181 taaattgaga aaagcccatt agtttatcta ctaatacttc taaaatttct tgctttcttc
    39241 tttaacatcg tcccgtaagg aatcatgtag tatgctggag gaagttcatt ttctaaataa
    39301 ttgtatcgct tcaattgctc aaaggctatt ctctacaatt aattacacca attactttat
    39361 cttcacttat ataagatttc taaacatttt tcgtcaaatg aagagaaaat atttttttaa
    39421 atgatcttgc tcaagatttg tcaacgaatc tttgaattcg ttttgaagtt ctgaggaagg
    39481 tatcatttaa aaactcaatc tgactttcat catttcgttt actcccattt tgatcacctt
    39541 ctaccaacaa tttattaaag tgattatact aggtttttat ttttttaatt cacaattgtt
    39601 tattcacttc atgaacaatt aaacacaaaa tcagt
//
LOCUS       DANJLF010000019        38904 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000019 DANJLF010000000
VERSION     DANJLF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(59..187)
                     /locus_tag="PWT25_001105"
     CDS             complement(59..187)
                     /locus_tag="PWT25_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL1021204.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="PWT25_001106"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="PWT25_001106"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL1021205.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="PWT25_001107"
     CDS             complement(1674..2729)
                     /locus_tag="PWT25_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL1021206.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="PWT25_001108"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="PWT25_001108"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL1021207.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="PWT25_001109"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="PWT25_001109"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL1021208.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="PWT25_001110"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="PWT25_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL1021209.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="PWT25_001111"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="PWT25_001111"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL1021210.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="PWT25_001112"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="PWT25_001112"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL1021211.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="PWT25_001113"
     CDS             10491..12050
                     /locus_tag="PWT25_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL1021212.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="PWT25_001114"
     CDS             complement(12161..14092)
                     /locus_tag="PWT25_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1021213.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="PWT25_001115"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="PWT25_001115"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL1021214.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="PWT25_001116"
     CDS             complement(15021..15773)
                     /locus_tag="PWT25_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1021215.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="PWT25_001117"
     CDS             complement(15932..16141)
                     /locus_tag="PWT25_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021216.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="PWT25_001118"
     CDS             complement(16152..17480)
                     /locus_tag="PWT25_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021217.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="PWT25_001119"
     CDS             17616..17771
                     /locus_tag="PWT25_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021218.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="PWT25_001120"
     CDS             complement(17845..18432)
                     /locus_tag="PWT25_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL1021219.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="PWT25_001121"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="PWT25_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL1021220.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="PWT25_001122"
     CDS             complement(20012..21301)
                     /locus_tag="PWT25_001122"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL1021221.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="PWT25_001123"
     CDS             complement(21455..22387)
                     /locus_tag="PWT25_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021222.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="PWT25_001124"
     CDS             complement(22544..23203)
                     /locus_tag="PWT25_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL1021223.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="PWT25_001125"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="PWT25_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL1021224.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="PWT25_001126"
     CDS             complement(24244..24534)
                     /locus_tag="PWT25_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021225.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="PWT25_001127"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="PWT25_001127"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL1021226.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="PWT25_001128"
     CDS             complement(26820..28175)
                     /locus_tag="PWT25_001128"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL1021227.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="PWT25_001129"
     CDS             complement(28199..29368)
                     /locus_tag="PWT25_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021228.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="PWT25_001130"
     CDS             complement(29365..30252)
                     /locus_tag="PWT25_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL1021229.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="PWT25_001131"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="PWT25_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL1021230.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="PWT25_001132"
     CDS             34332..34853
                     /locus_tag="PWT25_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL1021231.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35552..36109)
                     /locus_tag="PWT25_001133"
     CDS             complement(35552..36109)
                     /locus_tag="PWT25_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021232.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(36288..36899)
                     /locus_tag="PWT25_001134"
     CDS             complement(36288..36899)
                     /locus_tag="PWT25_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021233.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(37012..37269)
                     /locus_tag="PWT25_001135"
     CDS             complement(37012..37269)
                     /locus_tag="PWT25_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021234.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(37866..38144)
                     /locus_tag="PWT25_001136"
     CDS             complement(37866..38144)
                     /locus_tag="PWT25_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021235.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(38178..38429)
                     /locus_tag="PWT25_001137"
     CDS             complement(38178..38429)
                     /locus_tag="PWT25_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021236.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        10628 a         8397 c         6824 g        13055 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    34921 cggaagcaga agcaactcct tcttatttct tggagttaaa cgcttctgtc ccagcctcct
    34981 tattccgaat aaatggtgtc caaaagcttg catctgccag attatctcat ttcttatgac
    35041 aagctaagca aattatctcc accaaataat ttattcatat ttttgacttg cgcatactaa
    35101 ctaattctta aattttcata caaaatttct caatttccct taacgagtaa tatttatgta
    35161 cggtgattct cactagtatc ccaatcactt tttaagttat cccgatttca attctacttc
    35221 aaaaatagtt gcttttatgt atgctgttag ttgattagat ggtcatttgt tgagctaggt
    35281 cgtttatttt aaacaagttg tttctttagc ttatacctat ctcgtctacc aaacgcaaga
    35341 tggcaaacac aaatgcatac gcatctgtat ttgccacttt gattcgtata aagctaacaa
    35401 ctattaactc ctctcagcta atagttgctc tcttttttat tcagattcat ttttgctgat
    35461 cacagcctag tatttcaatg gttttgaaca ctaaaccaaa ttgttccatt acaacattca
    35521 ctggctgatg aaatggatta gtagatttcg attattcaaa gcgattttct attggtggat
    35581 cgtgttccca actaataaga cggattccaa acctatcgta cagatcttct aaaccactta
    35641 taaattttgc acctatttca ggtgttttat tgcgtggata ttctattgtt acgccattaa
    35701 atggatacca attaggatta ggctctcttt ttttctgtac atctcccata gaatagttag
    35761 cactttctgg gacataatat attcttcgac catcctctaa ttcatctaat aagatatttc
    35821 gtgatcgtct atcacttgct aaatgtagac gccacgtgcg ataccatgcc atgctacctg
    35881 catctgcaga aaatccatca aataatgcaa caattgtatc ttctatcaca atcctttccc
    35941 catctacttc atattctaat ctaaaatcaa actcccctcg ggtaatttct ggtcttggtg
    36001 gcttaggacc caatattatt ctacttaaaa ccgcattcgt tcccagatga aacacagcta
    36061 taacaacaat aatgattaat attttgtttc tatttgataa atttctcatt attcgcacat
    36121 cacctctaca ttctcacata ttagttcgta atactccttt aatatcaaaa tatctactgg
    36181 tcgtaataat attcgctatg aattcctact actcgtataa gaaaggccaa atcatcagct
    36241 tttacttgct gatgatttgg cttgtttcta tttatcgtaa ttgttttcta atcaccaaaa
    36301 ttgttaacaa tcggttcaga tatttcccag ttgattaagg taattccgta ttgtgtcaaa
    36361 acttcttgcg cttctccgaa attaagtaca gttcttatgt atccactttc tcctaatata
    36421 aagtctacct ctcttttaaa accttcctcg agatttctaa gggctatatg tggttttaat
    36481 gacattgatt gtacattttc ccccatataa tattgcgcac ttcctcgact aaaatagatt
    36541 cctagatcgt ccccaaaagg tagatcttct ctaccactag ctaatctact tctccatctt
    36601 cgttctcttt tcatagaacc tgcgctgaac tcacacccgt taaactcagc tatgacagta
    36661 tcttcaatta tgttaatctg tccatctagt tcgtattcaa tcctaaatgg aaattctcca
    36721 taggtgattt ccggttcggg tattttaggt gagattaatg caaagaaagg aaatattaag
    36781 cttggcaaca tcaaaaaaac tcctcctatg atcacaattc ccgatgtaat ccaaaatata
    36841 atttgtacaa ttctcattga tccattgtat tccattttat tccccctatt actattcata
    36901 tccgcctatc tatatcgatc ttccaatgca gagataattg cttccatacc ttgatttttt
    36961 actgagttac ctatgcgaga tatcattctc cactaaggtg catcatattg tctatctaac
    37021 atgattaatt ctccaatagg tgtatactcc tccaaaaaca acatttaatg catctccctc
    37081 gacaacataa gctaccatgt ttgctgtata tgtgtttgag tctaattcat aagcagataa
    37141 atttactgtc gctggattaa atataatcgc attcgtattt atagctactg aatttgctgc
    37201 ttcagcacca ccttttgaat gtccgatcat tgtgatttca atagtaggat ttacgtggac
    37261 agccctcacc gtttaccctg accattatag aaatcgctta cttttttgaa ctacgcaaca
    37321 aaggatacaa tgctcctaac ttcactgtct ccatttttgt ccactaagga atataaaaaa
    37381 taggcattaa ttcatttaat tcggtcacaa cacgttacct tttaaatcat tatactgtat
    37441 ctcatacatt attatcaata aactattata aaaatgcaaa aaatgcttta tcaatctatt
    37501 ttgtaattgg tcataatatc attgaaatca taccctgtat cgatttttcc tatgaatctc
    37561 gtctatttgt gattgacgaa aaagaggcaa gacagacact ggaatggtaa ttttacccac
    37621 tattttgctc caactatttg taatcgtacc cacttttgaa ataccgagat gatcatttta
    37681 attgatatgc tgattcccaa gcaaaacccc aagaaattaa atgctctatc gcatctagct
    37741 tcttgggttt tgcttatata aattccaaca tttaaatttt ttatgctgtt cggtctcaga
    37801 aatgccgttt ttgcttatat cacaatatca agatgtcaaa ttattcgtgt tgccgatgac
    37861 tttgattaat ttttttcaaa ttgtttatta agactaatgc tgtctttttg cagataccaa
    37921 tgttcgaact ttttaacatt atcatatgat cctgcaagtt ttaacttctt accattagat
    37981 gttatcaaag ttataattaa ccttgccttt ttcccattag gatcttttat tttatgaatt
    38041 ttctgcaggt aatctctcct aatggcagct atttttacct catcagtgct gataaaccat
    38101 tgatcatcca catacatact gcctgttatg ttgtgttttt ccatttcttc cgagaacgaa
    38161 aagtttaagg ctcgttctta cttccaaagt tgaatgagtc catggattgg acttagtgtg
    38221 agaattgaga acaacaataa gaatccatat cctcccgcaa acatccctaa ggctatactg
    38281 tctctccatc cataagtagg attagaagag aaaagagtca ccgtttgtaa taatatgaaa
    38341 aggaataatc cagcacaaac accaaagata acaactccaa acattgaatt tttatacaat
    38401 gaccatttat ttataggctt ttctttcatt ttacttcccc ctactgttct gttcacacgg
    38461 aatgaattca aagttcataa gccctcctct ttgaacatat gaatacgact tgctaactat
    38521 actttaaaaa agcttgtttt aagcggttat aggcgagttg taatctctat tcatctcatt
    38581 agcaaataac ttgccgaaaa gtctactgaa attaaagcag atagtggaag aattatttag
    38641 ccaggataga cactatttgt gaaacacatg tctttacttt tgaaattcta tgatgaaaag
    38701 ccttggaata cagtagctct taattggtaa tactttttcc attataaata acctcttttc
    38761 attttatatt tatcccctct aaaaataata tctaatctaa tattttaaac ttatatttcc
    38821 ctcgctgtgc taatagagca ttactaaata aatagaaact agaaaaaagc attacgcctg
    38881 ccagaataaa ccaattattt gtat
//
LOCUS       DANJLF010000020        37504 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000020 DANJLF010000000
VERSION     DANJLF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(160..324)
                     /locus_tag="PWT25_001138"
     CDS             complement(160..324)
                     /locus_tag="PWT25_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021237.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            619..1947
                     /locus_tag="PWT25_001139"
     CDS             619..1947
                     /locus_tag="PWT25_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL1021238.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2149..2982
                     /locus_tag="PWT25_001140"
     CDS             2149..2982
                     /locus_tag="PWT25_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL1021239.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2998..3735
                     /locus_tag="PWT25_001141"
     CDS             2998..3735
                     /locus_tag="PWT25_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL1021240.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3890..4858)
                     /locus_tag="PWT25_001142"
     CDS             complement(3890..4858)
                     /locus_tag="PWT25_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL1021241.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5090..5923
                     /locus_tag="PWT25_001143"
     CDS             5090..5923
                     /locus_tag="PWT25_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL1021242.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5957..6796
                     /locus_tag="PWT25_001144"
     CDS             5957..6796
                     /locus_tag="PWT25_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1021243.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6822..7382
                     /locus_tag="PWT25_001145"
     CDS             6822..7382
                     /locus_tag="PWT25_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1021244.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7581..9302
                     /locus_tag="PWT25_001146"
     CDS             7581..9302
                     /locus_tag="PWT25_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL1021245.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9467..10609
                     /locus_tag="PWT25_001147"
     CDS             9467..10609
                     /locus_tag="PWT25_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL1021246.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10625..11836
                     /gene="thiI"
                     /locus_tag="PWT25_001148"
     CDS             10625..11836
                     /gene="thiI"
                     /locus_tag="PWT25_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL1021247.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12409..13056
                     /locus_tag="PWT25_001149"
     CDS             12409..13056
                     /locus_tag="PWT25_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL1021248.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13185..13401
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13449..16094
                     /locus_tag="PWT25_001150"
     CDS             13449..16094
                     /locus_tag="PWT25_001150"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL1021249.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16404..17717
                     /locus_tag="PWT25_001151"
     CDS             16404..17717
                     /locus_tag="PWT25_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL1021250.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17707..18360
                     /locus_tag="PWT25_001152"
     CDS             17707..18360
                     /locus_tag="PWT25_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1021251.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18415..18966
                     /gene="radC"
                     /locus_tag="PWT25_001153"
                     /pseudo
     CDS             18415..18966
                     /gene="radC"
                     /locus_tag="PWT25_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19097..20182
                     /locus_tag="PWT25_001154"
     CDS             19097..20182
                     /locus_tag="PWT25_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1021252.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20179..22071
                     /locus_tag="PWT25_001155"
     CDS             20179..22071
                     /locus_tag="PWT25_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1021253.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22078..22455
                     /locus_tag="PWT25_001156"
     CDS             22078..22455
                     /locus_tag="PWT25_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1021254.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            22609..23391
                     /locus_tag="PWT25_001157"
     CDS             22609..23391
                     /locus_tag="PWT25_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDL1021255.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(23517..24248)
                     /gene="erm(A)"
                     /locus_tag="PWT25_001158"
     CDS             complement(23517..24248)
                     /gene="erm(A)"
                     /locus_tag="PWT25_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDL1021256.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(24306..24365)
                     /locus_tag="PWT25_001159"
     CDS             complement(24306..24365)
                     /locus_tag="PWT25_001159"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDL1021257.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            24757..25419
                     /locus_tag="PWT25_001160"
     CDS             24757..25419
                     /locus_tag="PWT25_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1021258.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            25654..25797
                     /locus_tag="PWT25_001161"
                     /pseudo
     CDS             25654..25797
                     /locus_tag="PWT25_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            25947..26147
                     /locus_tag="PWT25_001162"
     CDS             25947..26147
                     /locus_tag="PWT25_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1021259.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(26401..28080)
                     /locus_tag="PWT25_001163"
     CDS             complement(26401..28080)
                     /locus_tag="PWT25_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL1021260.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(28082..28294)
                     /locus_tag="PWT25_001164"
     CDS             complement(28082..28294)
                     /locus_tag="PWT25_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL1021261.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            28687..30417
                     /gene="efrA"
                     /locus_tag="PWT25_001165"
     CDS             28687..30417
                     /gene="efrA"
                     /locus_tag="PWT25_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL1021262.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            30414..32174
                     /gene="efrB"
                     /locus_tag="PWT25_001166"
     CDS             30414..32174
                     /gene="efrB"
                     /locus_tag="PWT25_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL1021263.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            32269..32466
                     /locus_tag="PWT25_001167"
     CDS             32269..32466
                     /locus_tag="PWT25_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021264.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            32619..33104
                     /locus_tag="PWT25_001168"
     CDS             32619..33104
                     /locus_tag="PWT25_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL1021265.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(33208..33801)
                     /gene="clpP"
                     /locus_tag="PWT25_001169"
     CDS             complement(33208..33801)
                     /gene="clpP"
                     /locus_tag="PWT25_001169"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL1021266.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            33981..34508
                     /locus_tag="PWT25_001170"
     CDS             33981..34508
                     /locus_tag="PWT25_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021267.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            34599..35336
                     /locus_tag="PWT25_001171"
     CDS             34599..35336
                     /locus_tag="PWT25_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL1021268.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            35340..36257
                     /gene="miaA"
                     /locus_tag="PWT25_001172"
     CDS             35340..36257
                     /gene="miaA"
                     /locus_tag="PWT25_001172"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL1021269.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            36259..37488
                     /gene="hflX"
                     /locus_tag="PWT25_001173"
     CDS             36259..37488
                     /gene="hflX"
                     /locus_tag="PWT25_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL1021270.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        12581 a         6175 c         7851 g        10897 t
ORIGIN      
        1 ggttcgagtc ctactgccgg agtaaataaa gcttatgcaa agaaaaagaa atccaatcac
       61 ttgggtttct ttttctttta ttattatgat gaatcaagct ttctgttttt gtcacgtatg
      121 ttgtaatctt tacgaaagag tagagttggc tactcataat caaccatact tcccatcatc
      181 gctaaatacg aaataaatca gcattcctac accaatcaaa gcacaagctg caccaagaaa
      241 agggatgaca ttcccaaagt tatctgtatt cataagtatg ccaccaccca caaacaaaaa
      301 gccgccagca atcaggtttt tcattttatt catctccttt cttacgttat agtatttcct
      361 tctttcaatg agtattttga aagctatctt attgtatgat gttcttctag gcctcttctt
      421 tattatacag gccattttcg ttatctcata gaaatattcc tttttatctg aaaactattg
      481 tataccgtat gacatttttc ttcatgaagt tgtaagtata ttctcattca aatgaaaatt
      541 tcatcataga agagattgta tttctaaatt cttaagagta aaatacgaag tataacttgg
      601 atttggaagg atgattggat gaaagctatt acgcctgata tgacaacaaa atttaacaaa
      661 cagtttcaag caaatatcaa acagcaggca ttgcagcggg cagttgtgaa gaacggaatt
      721 actgcttctg ctgaaaatgt ttcagcacat gtggagaata caccggtatt ttctatagat
      781 ttggctacgg ggaaagtggc taatcaaatg caatcggggc gttgctggat gtttgctgca
      841 ctgaacacat tccgtcataa gatgctgaat caattccatt taaaagagtt tgaattatca
      901 caaaattaca cttttttctg ggataaatat gaaaaggcaa attatttcta tgaaaacatc
      961 attgcaacag gaaaagaggc aatcgatagc cggaaagtag ctttcttact cgctactcct
     1021 caacaagatg gcggccaatg ggatatgatc gtttcattat tccaaaaata tggcgttgtg
     1081 cctaaatcgg tgatgccgga aagtgcgaat agttcaaatt ctcgagattt gaataactac
     1141 ttgaacaaat tactgcgtaa agatgcagta gtgttacgga aaatggttgc tcaaggagaa
     1201 acattagacg ctattgaaga aaagaaagaa gaaatgctag aaagcattta taacttctta
     1261 gctatctcat taggaacacc tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag
     1321 aactatcatc ttgaccgagg actgacacca caaatttttt atgataaata catcggtgta
     1381 aaattagatg attatgttag cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt
     1441 tcttatacag tagagatgct aggaaatgtc gttggcggga aagaagtcaa atatctgaat
     1501 gttgatatgg aaactttcaa aaaattagcg attgctcagc ttgaagaagg agaatctgtt
     1561 tggtttggtt gtgatgtagg tcaatcttct actagaacta gtggcatcat ggccttagat
     1621 gcttattcaa tggatgatct atttgatacg gattttacaa tgactaaagc tgaacgttta
     1681 gattttggag aaagtctgat gacccatgcg atggtattga ccggtgttga cttagtagac
     1741 gggcaatcaa caaaatggaa agtcgaaaat agctggggcg aaaaagttgg aaagaatggc
     1801 ttctttgtta tgagtgatgc ctggatggac gaatatacgt accaaatcgt tgtaagaaaa
     1861 gatttattga cgaaagagca attaaatgcg ttcaatgaag aaccaattgt tctatctccg
     1921 tgggatccaa tgggtgcttt agcatagacg agtatttcaa tgaagagtat gtagtaaaga
     1981 taaaaagagc tggcttaagc cagctctttt tatctttaaa gtccattccc tgttttttat
     2041 ggtccctgtg ctataattct aaataaagga taaggattgg aaataatcct tttatcatta
     2101 aaaatatgat gacgaagtag aataaaattg ggagtagggg ggaaagtcat ggatatccaa
     2161 actgttttat ctgatctgaa attaaatggt aaagtgattc cagttgttgg tggcgatgtt
     2221 aatcagacct atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat
     2281 gtaaaaaaag gattttttga agcagaagta gacggattaa aagaactgtc agcattcgta
     2341 cgtgtaccgg atacgtatat gctaggtgaa acaagtgaag gcgcttattt gttaatggaa
     2401 tggatcgagc cgggaaaagg ggatcaacga gatcttgctg cagctttagc taatctccat
     2461 cagcaaacag cgccacaatt tggttttcgt aaagataact atttgggtac acttgttcag
     2521 aaaaattctt ttgaagaaga ttggtggact tttttcttta aagatcggct tgaatcccag
     2581 atttctttgg cagaagaaac gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga
     2641 tttaaagaac gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg cctcttgcac
     2701 ggagacctgt ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac
     2761 ccagctgttt cttatgggaa tcgggaacaa gatattgcaa tgagccagtt attcggtggt
     2821 ttccgaccgg aatttttaga tgcttatcaa actatatttc cattagaaaa gggatggaaa
     2881 gaccgactgc cgatttacca gttatattat cttttggcac atttgaatat gtttggagaa
     2941 agttacggtt cccaagtaga tcaattacta gaaaattttt aagaggaggg cgtgtttttg
     3001 gcaaaaaaat ataccaaaaa gcatgaagtt tcatattatg agtgtgacat caaccaaacg
     3061 atgacatttc cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca aagcgaccag
     3121 ttgggaagag gcagtgaatt cgtcaatagc tttggactta cttgggtcat cacgaattat
     3181 tccatggaga ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc
     3241 atggagtaca ataagttttt ctgttatcgg aacttctgga tccatgatga agatgggaaa
     3301 gaattagtca aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc
     3361 agtgttaatg aagagatcat tgctcctttt gagagtgaga agatcaagaa aatcaagcgt
     3421 caggaaaaaa ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt tcgtttttat
     3481 gatatcgata gtaatcagca tgttaataat gcgatgtatt ttaattggat cattgacgta
     3541 ttaggatatg actttttgac tacgcatgtt ccagaaaaag taattattcg atttgataaa
     3601 gaagtagagt acgggaatga gatcgaaagc cattttgaac aaatccctat cgaaaatggt
     3661 gtcaaaacaa aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc aaatattata
     3721 tggaaaaata aataaaaaag aggttgtgac agaagggggc aacttcaaga aataagaaga
     3781 aatttacgaa aattgtttct caaatttttg taaattttcg acttagtttt cgaagaagct
     3841 atttttgtca cagcctctct ttttaagttt agctattaga taatgatgtt tagttttgga
     3901 taaacgaaat caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac
     3961 gtgctttttg actattaata ctgttacaga aaataatccc cattttttct agttcttttc
     4021 ccccgccttc ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta
     4081 ctacagggat tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat
     4141 tccccgcacc taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca
     4201 ataagtcccc ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcgg
     4261 aaatgtccag tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta
     4321 ctaagccgat gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct
     4381 ttgtcacgta gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta
     4441 gcgcttcttc acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc
     4501 caagttcatt acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg
     4561 ttgtatcaag aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc
     4621 cggatatacc tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag
     4681 gcatgatgtg tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg
     4741 ggattgtgat ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga
     4801 gcatgttttc tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt
     4861 tacagacttc cttacgaata attttgcata gccgttaaat cttattgtat actatataaa
     4921 agagttttgc tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct
     4981 tattaaaatg gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt
     5041 tcttttacaa gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat
     5101 cggattaaca tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta
     5161 tgaatatgca gggtttctac ccttagtgga attagatact gcctattatg gaattccccg
     5221 aaagtcctca gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa
     5281 agtctatcaa ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga
     5341 gatgattgcg cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg
     5401 ctttttagtc caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg
     5461 caaacttaga aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg
     5521 gtacagcgat gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt
     5581 agccatcatc gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac
     5641 caatccgaac tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa
     5701 tgataaagag tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga
     5761 actagcacat gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa
     5821 taataattct ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat
     5881 caagccagat ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa
     5941 gaaaggggcg aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac
     6001 caatcgttca aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg
     6061 cattttatgc ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga
     6121 attggaatta gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac
     6181 gattatcgac cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga
     6241 gaaccatcga caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct
     6301 attaggacaa tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt
     6361 acgtctgatg aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga
     6421 aacgttagag atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga
     6481 acagaaaaag ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac
     6541 tgtagatatc atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc
     6601 tgtcgaaatt gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat
     6661 gctgaaaggt gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc
     6721 tgcagacttt gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt
     6781 cggtctgatt tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt
     6841 ttcaaaaagt agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg
     6901 cttttacaga aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat
     6961 ttgttgcagc tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg
     7021 aagcattaga taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc
     7081 tggttgaaca agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat
     7141 tactaggaat agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat
     7201 tatttccaga tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa
     7261 actggcagat ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc
     7321 aagaagcgat tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt
     7381 gatcagagtt tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt
     7441 aagttagtga atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag
     7501 agacgtatta tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac
     7561 tgaaaacgga ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca
     7621 ttatagtcgc cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc
     7681 gattggatgc cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag
     7741 tcgatgatgt taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga
     7801 gtcaaaaatg gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg
     7861 aagtggaaaa cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag
     7921 cagaagctac aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg
     7981 aattgcggga aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg
     8041 aagaactgaa agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat
     8101 tgcaaaaaca gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt
     8161 ctggagaccc tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag
     8221 tggaagctct gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg
     8281 aacaacttga agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc
     8341 cagaagaaga tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag
     8401 ccaatcttga aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt
     8461 tgattgattc gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa
     8521 ctaaccaatc aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat
     8581 tgattgagct ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg
     8641 tacgaggttt ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac
     8701 agatccgtca acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca
     8761 aagtcttaga agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga
     8821 gaaaaggcga aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt
     8881 tgaaacgttt tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct
     8941 tttttgttgc tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa
     9001 acatggaaga ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata
     9061 agaagacaca tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa
     9121 atcgttatcg tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat
     9181 tcaataaaga atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag
     9241 tagaacctgg tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat
     9301 aaagcaaaaa agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt
     9361 tttttgtata tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg
     9421 gacaaccgtt ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga
     9481 taacagtgca acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca
     9541 gcggattatt gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt
     9601 gcagcaagca agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt
     9661 tacttcaggc ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag
     9721 agaatatggg aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc
     9781 agaacaatta aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt
     9841 tgtacgtgtc gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat
     9901 ggctgtgaat aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga
     9961 tgattttcct aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt
    10021 ctcgcaatgg ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg
    10081 cccacgggga actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac
    10141 aggtggaggg caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc
    10201 tatggttaaa gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca
    10261 acgcttaaaa gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc
    10321 agaaggaact gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga
    10381 agttctcgtc catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc
    10441 ctcacgtaaa catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc
    10501 tacatctgct gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt
    10561 tatgattatt ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt
    10621 tcttgtgaaa tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa
    10681 tcgaaaaagc tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc
    10741 agaaatcaaa atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg
    10801 gcagctagtt attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat
    10861 tcgtgtagaa aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat
    10921 ttatactggg aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt
    10981 gacaagtaat gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat
    11041 ccaagttcaa atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc
    11101 ttatctatcg tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa
    11161 aggaatgctt atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa
    11221 gcggggagtg gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc
    11281 actgcaaaaa gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt
    11341 tatcgaagtg ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata
    11401 ttggatgaca attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg
    11461 tcatggttta gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca
    11521 aagtatggtg gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat
    11581 ggataaactt gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca
    11641 gccttttgaa gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact
    11701 ggaaaaggtc catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact
    11761 agctggcttg aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt
    11821 ttcagaattg ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa
    11881 aacgaaaaaa tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat
    11941 tgaataccat ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata
    12001 aaattttatt tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat
    12061 gaaatagcga ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa
    12121 attgcttttc aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac
    12181 accgtgtatc agaggttgcg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    12241 aaaatagttc ctcatatttt acgtttggtt gagcttatta ccgcagatgc aagcttttga
    12301 acaccgtttt atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta
    12361 gaaacatgtt acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa
    12421 acagatacac tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag
    12481 atgctggatg actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa
    12541 attccttctg caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga
    12601 tatggctatg atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa
    12661 gaaaaacgaa tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat
    12721 tttagaagaa atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc
    12781 aatcgagtaa ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa
    12841 gcaaaaaatg tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt
    12901 gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact
    12961 gtcccagctt atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt
    13021 tactttgatg agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat
    13081 cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt
    13141 tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca
    13201 agaggagtaa ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat
    13261 gacaaccagt gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc
    13321 gtgatcgtta actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg
    13381 cgtaaatcat acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg
    13441 aaaacagcat gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag
    13501 ctggacgtta tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag
    13561 caaaacctta ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc
    13621 atgcttggga tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg
    13681 atactttatg gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag
    13741 cgaagcttag agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc
    13801 aagtgtggga atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg
    13861 gcttatcttt agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag
    13921 tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca
    13981 tcaactggga tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata
    14041 tcgaaggcgc cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa
    14101 ttgcgacaac ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag
    14161 acgagcgtta tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa
    14221 tcccaatcat tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca
    14281 ctccagcaca tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa
    14341 atgttatgga tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac
    14401 gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa
    14461 tcgatacgat gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc
    14521 ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc
    14581 aagaaacaga tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt
    14641 ggatggaaaa tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc
    14701 ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata
    14761 gtgaaaactg ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc
    14821 ctttctcaac aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta
    14881 ctagcacttt agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc
    14941 aaagcttgga atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc
    15001 gtgacgaaca agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag
    15061 taatcgaaaa gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag
    15121 gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa
    15181 tctggaatgc ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg
    15241 atttatctgg agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga
    15301 tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt
    15361 ataactttat ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt
    15421 atggagatga tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc
    15481 agatcctacg tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata
    15541 ttcctcatca gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg
    15601 atgatgaaac agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa
    15661 atattcgttc agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa
    15721 acgatgctaa aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta
    15781 atcccgaaga gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg
    15841 ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa
    15901 tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa
    15961 aactttctaa tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg
    16021 caaaagaaaa agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc
    16081 gtactattca ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct
    16141 agctgattga acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa
    16201 ggagatgtga cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga
    16261 aggagggagg tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc
    16321 tctgtttctt ttctctttgt catactttta tttttcttat gtgttctatt tgttataata
    16381 aaagagaaat gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc
    16441 gattaccatt tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag
    16501 ggcatccaga gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta
    16561 ctgtagctta tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt
    16621 caccttatat cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca
    16681 atgatttgat ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg
    16741 aagcagtagc tggtattact gaattcgaga cattaacagc aatggcattt gattattttt
    16801 tgatgaagaa agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta
    16861 ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa
    16921 tacttggaga tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg
    16981 ttccagtagt aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta
    17041 aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc
    17101 ctgatgagat gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat
    17161 taaccactcc gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt
    17221 attttgttta ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat
    17281 tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt
    17341 tagatggtgc ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat
    17401 tcaaaggata tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa
    17461 tgctagaacg attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc
    17521 caaaagccat cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt
    17581 cattgtggca attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag
    17641 ttgtgaccgg ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag
    17701 gagatcatga agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag
    17761 ttagtgtatt acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa
    17821 gacctgtatt taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg
    17881 attttctcgg aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa
    17941 acgcttgaac gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc
    18001 tcttttttag aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc
    18061 attgaacttt tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca
    18121 gaaaatgttc aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta
    18181 ggtacagcga agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct
    18241 tctgcggctg agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg
    18301 atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa
    18361 aaatccatct cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga
    18421 aaacgattga tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat
    18481 ggcgcggaag ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac
    18541 ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct
    18601 ttacgacagg ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca
    18661 atcgaaatca aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg
    18721 gctccaccgg ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat
    18781 cacaaacaag aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa
    18841 aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat
    18901 tatgctgtac gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat
    18961 gttttaacca tagtataata aataatagta gttaaagtag tttaagagga gatattaaag
    19021 ggatatttta ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt
    19081 aaagaggtgg gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt
    19141 gtatttatta ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata
    19201 cttagataat actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt
    19261 gctgtacgag ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt
    19321 agcagacttc gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc
    19381 aaaaaaagcc ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt
    19441 tcttgattat ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa
    19501 gggaagaaat ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt
    19561 cttaaagtta agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca
    19621 aattattgat gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg
    19681 tggtttaaga atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa
    19741 tcaaatccat ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa
    19801 ggaaagaaca atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata
    19861 tgagtatagt gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt
    19921 tgggaaacca ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac
    19981 tggaatagaa tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga
    20041 aggatgggat gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt
    20101 aaatacctat gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga
    20161 gagaaaggag cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag
    20221 attagcaaaa aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt
    20281 tgggatataa gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta
    20341 aggaatcgtt gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa
    20401 tatttttatt tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga
    20461 aaaggaacag taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact
    20521 tcaattactg aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa
    20581 cggggtgtta gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct
    20641 gtaaaagcta attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac
    20701 ttttattttg atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg
    20761 ccagatgata aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg
    20821 aatacatatt ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc
    20881 ttttcctatg taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg
    20941 aaatttaaac aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta
    21001 agtgaactaa acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg
    21061 gaaggactat ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta
    21121 atttattctg aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc
    21181 gtcctagagc aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact
    21241 atgattgttc aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt
    21301 ctattagagg acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag
    21361 gagcatatag ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa
    21421 gtttcagagg aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta
    21481 aaacaagaaa catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat
    21541 aaatcaggtg agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga
    21601 atgattaaca acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca
    21661 gaaatgacaa gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa
    21721 tttcaggaaa aactggttac caataatgga gatgtgcttg atctagatga agataatgaa
    21781 gtcgatgatg tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat
    21841 ggttattgta gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat
    21901 tgcactcact tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt
    21961 acagaagagt tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat
    22021 agccgtgtta aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg
    22081 gataaacaag ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag
    22141 acaagggaaa aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat
    22201 tttaattcaa tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat
    22261 attaggcaaa gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa
    22321 cccaagaaaa gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg
    22381 gaattagaaa aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg
    22441 tataataaag aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta
    22501 acggtcagct ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga
    22561 gtatcaatat aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg
    22621 attaacggaa aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt
    22681 ggaagttcaa tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt
    22741 aacagcgatg tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa
    22801 aaactaacag aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga
    22861 ccacttgaag ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa
    22921 agagaattta tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa
    22981 ccaagctatg atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct
    23041 ctatttggtc ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga
    23101 gcaattaagg attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta
    23161 attttaaccc tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat
    23221 gtcgctgcag aatgggctat acctctttta cctaaagagc atgtaacttt actggatata
    23281 gctagaaaag gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg
    23341 aaagcactcg ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt
    23401 attgcaataa caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt
    23461 tttgggaaga ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag
    23521 tgaaacaatt tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata
    23581 ttagtgacat ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga
    23641 tattcacggt ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa
    23701 atcaatggtt gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga
    23761 aaatatagtg gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa
    23821 cccaaagctc gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag
    23881 ctatatttag cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa
    23941 ggaatattac catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc
    24001 gtttgaatca cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag
    24061 cctccatcaa tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa
    24121 tgtccttttc ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc
    24181 aatatttctt ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc
    24241 tggttcatta taaccttctc cttactggtt ataatgaact gacattaagc gttcattata
    24301 accaattaat tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta
    24361 cccatacttt tatctccttt ccattttttt cattatgaat gagataaagt aatttctact
    24421 actgcgatac tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat
    24481 cttttcattt tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt
    24541 tttcttcaaa ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag
    24601 taaaggctgc gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga
    24661 taaaattgtc taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat
    24721 gaaagaatgt cgataaagta ggaatggggg atctttttga atacaataga atcacagttt
    24781 gataaggttg cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt
    24841 gtgtctaata tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg
    24901 ttagtggaga aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa
    24961 atgctcgatc ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat
    25021 gcagaacaac ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat
    25081 catttggatg atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga
    25141 agaatagtta ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa
    25201 ttaggggcaa tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt
    25261 agaatttata atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac
    25321 ctatctgaac aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat
    25381 aaaatgggtt gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa
    25441 agttgaattt gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata
    25501 aaaaatcagc ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt
    25561 ttgaaataag taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat
    25621 gattcttttg taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt
    25681 accaaaagga tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc
    25741 atcggacgca aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt
    25801 gctttcttgc aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata
    25861 gaagttgaaa catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt
    25921 gcttttggca cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg
    25981 aaaaaggatt tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag
    26041 ctatccaagg tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg
    26101 aagaatcaga ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa
    26161 tcaaattgat ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg
    26221 ccacaagaga caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt
    26281 aagaggctat gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc
    26341 atgtttttca tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat
    26401 ttattcttca tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc
    26461 gaataagaaa ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc
    26521 aggtccattc aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat
    26581 ttctcctgat tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat
    26641 ttcttttttc ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag
    26701 gtgacggtca cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac
    26761 atctccagcc acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc
    26821 gccattttcc atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc
    26881 atgtactttc aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa
    26941 acgaagcatt aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat
    27001 ttttccgtga atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact
    27061 tgttgtatta ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg
    27121 gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga
    27181 acctgtacat aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac
    27241 aaatgtgccg tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc
    27301 catgcttcgt ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg
    27361 ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga
    27421 aaagatcttc aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc
    27481 agcatttgta ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt
    27541 atgcaagttt gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac
    27601 gatatttcca gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt
    27661 tctgaaaaag ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg
    27721 gagctctgca tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc
    27781 gagaggacca gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat
    27841 gtggtcttcg tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat
    27901 gtagctgtag tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat
    27961 cccggcatca atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat
    28021 ttcacctaat ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat
    28081 attaaaactc cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt
    28141 ctagaagttg aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt
    28201 ctacatcgct ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta
    28261 cttttgtttc ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa
    28321 gcagtgattt agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg
    28381 tttttcatat gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg
    28441 cttatctgtt cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa
    28501 ttgttattct agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa
    28561 tcaatgttta tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta
    28621 ataggctcgt tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat
    28681 gagaaagtga aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat
    28741 ttcatatgcg tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt
    28801 atgatcgatg taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt
    28861 atgatcgtta ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg
    28921 aggattacga ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt
    28981 tattcgcatc aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac
    29041 gatgcttatc agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca
    29101 atgatgttca ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat
    29161 gtagtaggtg cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa
    29221 ccgttatcta aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat
    29281 ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt
    29341 gctgtagtca atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt
    29401 gcacaaccgg gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg
    29461 gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat
    29521 atattccatg ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga
    29581 gcagctgttt cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa
    29641 gatccaaatg gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca
    29701 tttgcttatc caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct
    29761 ccaggagaga ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa
    29821 ttgatccctc gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg
    29881 agagattatc ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg
    29941 ctatttactg gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa
    30001 gaaatggaac gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat
    30061 ggatttaatg aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga
    30121 ttggcaatag ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt
    30181 tctgcattag attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca
    30241 gaatctactg tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc
    30301 atcgtattaa atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa
    30361 tgcccgattt attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa
    30421 aaaccttttc ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc
    30481 ttttagtgat cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta
    30541 caggacttcc tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt
    30601 cttatatatt caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc
    30661 aattactttc tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa
    30721 gagacataga agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag
    30781 gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag
    30841 cttttattgg tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt
    30901 atatccagcc gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct
    30961 caaaacgagt gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg
    31021 agctaaacgg ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac
    31081 gcgaaaaaga aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt
    31141 ttcgtgcagc attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta
    31201 cttatattgg tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg
    31261 gtcaactaca agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga
    31321 cacaattgtc ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt
    31381 tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag
    31441 gagatgtgac attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag
    31501 atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg
    31561 gcaagacgac attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa
    31621 ggattgatgg aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta
    31681 tggtcttgca agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa
    31741 agcttgatgc gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt
    31801 ttatccgaac gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt
    31861 ctttaggaca aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct
    31921 tgattttaga tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag
    31981 caatggatcg cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa
    32041 tccgtgatgc agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc
    32101 atgagagttt attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg
    32161 aggaagcaga ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat
    32221 tcttgataat ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt
    32281 ttaaatgaaa aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag
    32341 acgaagcctt ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca
    32401 gcacaaatag aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta
    32461 caataaaaac aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca
    32521 ggttttacct gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag
    32581 gattagtttc tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa
    32641 atgccacagt gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg
    32701 ttttacgcgg ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta
    32761 caatcatcca tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac
    32821 atcagtgcat gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca
    32881 cgatattgaa tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa
    32941 cagaaggaaa agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc
    33001 gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga
    33061 taggtcggat gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa
    33121 aataaaaaag gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga
    33181 gcttaaaaaa gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc
    33241 atcgatcaat ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc
    33301 tttttcaatc acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc
    33361 gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc
    33421 gccaagaggt tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt
    33481 accagctgtc agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac
    33541 atctgctttg acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc
    33601 aggagaattg atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa
    33661 ttgggcgatg atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg
    33721 gtctttcaat aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat
    33781 gactgtagga attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac
    33841 cataatttag tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat
    33901 aaaaaactta ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca
    33961 aaacaaggag gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag
    34021 gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac
    34081 agcgtagacg tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata
    34141 aaaagatcaa cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg
    34201 atgcatttga tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag
    34261 cgcaaaatag tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc
    34321 atcgaaaagt gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag
    34381 gcggacgcgt gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa
    34441 aagctcagtt gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt
    34501 ggagctgata ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt
    34561 tcttaatgat acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc
    34621 gcggaagcag cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag
    34681 taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta
    34741 tgcacgacga agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat
    34801 tggaagaact caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta
    34861 aagttcctga attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc
    34921 taatgattga aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg
    34981 aaacattaaa aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt
    35041 ctttggaaaa aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt
    35101 ctgataaaaa gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa
    35161 atacttggtt gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat
    35221 ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa
    35281 taactgattt tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata
    35341 tggaaaaagt attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg
    35401 aactcgcaca actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa
    35461 atctggatat cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt
    35521 tgattgatgt gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc
    35581 gacaattgat taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg
    35641 gtctctatat ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc
    35701 ctgagattag aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga
    35761 atcagttgaa agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca
    35821 aagtgataag agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag
    35881 aagagccaaa gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc
    35941 tttatgaacg aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag
    36001 cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat
    36061 ttttccctta ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac
    36121 attcgagaag atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt
    36181 ggtttgatct tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat
    36241 ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca
    36301 aaccagcgtt acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca
    36361 ggagaagtcg tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc
    36421 ggtaaaggga aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc
    36481 atctttaatc atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt
    36541 ccggtaattg atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa
    36601 gaagggaagc tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg
    36661 caagggaaaa cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca
    36721 aagttagaaa cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg
    36781 aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt
    36841 cagattggac ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca
    36901 caagcggata cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga
    36961 tggcgttttg ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac
    37021 ctgcctacgc aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat
    37081 cttttgctac atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc
    37141 ttaaaattga tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag
    37201 gcagaccaga tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct
    37261 actcagacga aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa
    37321 ctaatgaccc cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg
    37381 agaagacaaa caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt
    37441 aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt
    37501 ttaa
//
LOCUS       DANJLF010000021        37245 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000021 DANJLF010000000
VERSION     DANJLF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..839
                     /locus_tag="PWT25_001174"
     CDS             <1..839
                     /locus_tag="PWT25_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1021271.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1263..1433)
                     /locus_tag="PWT25_001175"
     CDS             complement(1263..1433)
                     /locus_tag="PWT25_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1021272.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1627..1806)
                     /locus_tag="PWT25_001176"
     CDS             complement(1627..1806)
                     /locus_tag="PWT25_001176"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021273.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2193..3305
                     /locus_tag="PWT25_001177"
     CDS             2193..3305
                     /locus_tag="PWT25_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1021274.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="PWT25_001178"
     CDS             complement(3383..3676)
                     /locus_tag="PWT25_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL1021275.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="PWT25_001179"
     CDS             3844..4158
                     /locus_tag="PWT25_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL1021276.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="PWT25_001180"
     CDS             4179..6158
                     /locus_tag="PWT25_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1021277.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="PWT25_001181"
     CDS             6171..6497
                     /locus_tag="PWT25_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL1021278.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="PWT25_001182"
     CDS             6513..7031
                     /locus_tag="PWT25_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021279.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="PWT25_001183"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="PWT25_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL1021280.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="PWT25_001184"
     CDS             complement(8467..9972)
                     /locus_tag="PWT25_001184"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL1021281.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="PWT25_001185"
     CDS             complement(10064..10654)
                     /locus_tag="PWT25_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1021282.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="PWT25_001186"
     CDS             10833..12479
                     /locus_tag="PWT25_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1021283.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="PWT25_001187"
     CDS             12528..13247
                     /locus_tag="PWT25_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL1021284.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="PWT25_001188"
     CDS             13726..14673
                     /locus_tag="PWT25_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1021285.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="PWT25_001189"
     CDS             14685..15605
                     /locus_tag="PWT25_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1021286.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="PWT25_001190"
     CDS             15646..17103
                     /locus_tag="PWT25_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1021287.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="PWT25_001191"
     CDS             17448..18062
                     /locus_tag="PWT25_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL1021288.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="PWT25_001192"
     CDS             18055..19779
                     /locus_tag="PWT25_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1021289.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="PWT25_001193"
     CDS             19776..21227
                     /locus_tag="PWT25_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1021290.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21700..23853)
                     /locus_tag="PWT25_001194"
     CDS             complement(21700..23853)
                     /locus_tag="PWT25_001194"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL1021291.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23855..24901)
                     /locus_tag="PWT25_001195"
     CDS             complement(23855..24901)
                     /locus_tag="PWT25_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1021292.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25051..26340
                     /locus_tag="PWT25_001196"
     CDS             25051..26340
                     /locus_tag="PWT25_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL1021293.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26353..29055
                     /locus_tag="PWT25_001197"
     CDS             26353..29055
                     /locus_tag="PWT25_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL1021294.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29040..29897
                     /locus_tag="PWT25_001198"
     CDS             29040..29897
                     /locus_tag="PWT25_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1021295.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29968..30867)
                     /locus_tag="PWT25_001199"
     CDS             complement(29968..30867)
                     /locus_tag="PWT25_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL1021296.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31036..33207
                     /locus_tag="PWT25_001200"
     CDS             31036..33207
                     /locus_tag="PWT25_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL1021297.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33275..33616
                     /locus_tag="PWT25_001201"
     CDS             33275..33616
                     /locus_tag="PWT25_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL1021298.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33892..35646)
                     /locus_tag="PWT25_001202"
     CDS             complement(33892..35646)
                     /locus_tag="PWT25_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1021299.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35657..36493)
                     /locus_tag="PWT25_001203"
     CDS             complement(35657..36493)
                     /locus_tag="PWT25_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL1021300.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36495..37178)
                     /locus_tag="PWT25_001204"
     CDS             complement(36495..37178)
                     /locus_tag="PWT25_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL1021301.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12016 a         6551 c         7639 g        11039 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgagg
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    21241 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    21301 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
    21361 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
    21421 actcgaaact aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc
    21481 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    21541 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
    21601 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
    21661 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
    21721 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
    21781 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctctgtc
    21841 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
    21901 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
    21961 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
    22021 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
    22081 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
    22141 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
    22201 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
    22261 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
    22321 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
    22381 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
    22441 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
    22501 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
    22561 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
    22621 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
    22681 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
    22741 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
    22801 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
    22861 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
    22921 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
    22981 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
    23041 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
    23101 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
    23161 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
    23221 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
    23281 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
    23341 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
    23401 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
    23461 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
    23521 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
    23581 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
    23641 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
    23701 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
    23761 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
    23821 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
    23881 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
    23941 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
    24001 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
    24061 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    24121 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    24181 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    24241 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    24301 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    24361 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    24421 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    24481 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    24541 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    24601 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    24661 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    24721 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    24781 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    24841 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca
    24901 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    24961 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    25021 gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg
    25081 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    25141 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    25201 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    25261 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    25321 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    25381 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    25441 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    25501 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    25561 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    25621 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    25681 atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca
    25741 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    25801 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    25861 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    25921 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    25981 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    26041 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    26101 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    26161 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    26221 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    26281 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    26341 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    26401 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    26461 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    26521 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    26581 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    26641 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    26701 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    26761 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    26821 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc
    26881 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    26941 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    27001 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    27061 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    27121 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    27181 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    27241 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    27301 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    27361 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    27421 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    27481 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    27541 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    27601 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    27661 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    27721 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    27781 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    27841 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    27901 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    27961 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    28021 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    28081 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    28141 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    28201 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    28261 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    28321 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    28381 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    28441 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    28501 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    28561 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    28621 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    28681 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    28741 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    28801 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    28861 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    28921 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    28981 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    29041 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    29101 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    29161 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    29221 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    29281 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    29341 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    29401 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    29461 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    29521 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    29581 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    29641 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    29701 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    29761 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    29821 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    29881 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    29941 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    30001 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    30061 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    30121 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    30181 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    30241 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    30301 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    30361 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    30421 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    30481 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    30541 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    30601 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    30661 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    30721 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    30781 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    30841 agtgcaattg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    30901 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    30961 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    31021 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    31081 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    31141 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    31201 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    31261 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    31321 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    31381 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    31441 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    31501 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    31561 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    31621 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    31681 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    31741 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    31801 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    31861 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    31921 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    31981 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    32041 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    32101 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    32161 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    32221 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    32281 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    32341 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    32401 cctgctgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    32461 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    32521 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    32581 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    32641 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    32701 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    32761 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    32821 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    32881 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    32941 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    33001 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    33061 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    33121 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    33181 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    33241 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    33301 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    33361 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    33421 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    33481 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    33541 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    33601 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    33661 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    33721 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    33781 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    33841 attcagaaat aggagctacg acaaaagtct tgtcatagct cctatttttt gtcattcatt
    33901 tttcaagcgg tagaaatagc ctttttgagc taacagtttt tcgtaagtac ctatttcaac
    33961 gatttttcct tcatcgatca caacgatttt atctgcctct ttgatcgttg aaagccgatg
    34021 agccacaata aaagtagtgg gtgtttctgt caggtaattc agggcttgct ggattttttt
    34081 ctctgactgg ttatctaaag ctgaggttgc ttcatctagc aaaatgattt ttggctgacg
    34141 tatcaacgct cgtgcaatgg agatacattg cttttggcca ccagacagct tgttgccatg
    34201 ttcgcctact aaggtatcca gaccatcagg aagctggtca atcagtgaag aaagttgcgc
    34261 agcttcgatg acttcatcta aacgttcttt tgatacgttt ttcaagccat aagtgatgtt
    34321 ctctttgatc gaagcggaga acaacaaagt ggtttgagga acaacggcta aataatttcg
    34381 cacacttcgc atatccagtt catccaaagg ctgatcatct agaaaaattt ttcctgaagt
    34441 cggctggata aatccaatca ataagttaag gagtgtagat tttccagagc cagatggccc
    34501 aacaaaagca attgtttctc cagctttcac ctctagagaa aaatgatcga taatcggttt
    34561 gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga aaatttccat ccaaatgttg
    34621 gatttttttc ttaccgtgat aagtttctac atcgccagca atcaacagtt cgccaataga
    34681 agtgatagat tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc cagaaacgga
    34741 attgacgatc gtcgtgaaat acgtctgata catcacgaca tcgccgggtt ggatgcgccc
    34801 ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc tgaaatatct gaaaactagc
    34861 ccagctgact gatccgaaaa cagattgcag gatatctaag tggaatcctt tttcatagat
    34921 ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt tctaacccat gagctcgagc
    34981 aatcggtgct aattcgatca cttcgatcac ttttgcagaa gtctcttcca tttcttgcct
    35041 gaagtctgag ttggactgtc tgatcctttt acggaaaaag agagtaacga atgcggcgag
    35101 aggaacagtc aataagaaaa acatgaaaac aacaggactt ttataaagcg tgatacctaa
    35161 agctacgaat aaattgacag cgatatttaa tccgctgaca aatacttgag aggacaacgt
    35221 ttggatagct tctacatcac ggatgatctt tgactggatt cgtccagatt gcaattcttt
    35281 ttgataagga atagatagtt cttggatttt cttgataagg gtcgctctta gtccagcttc
    35341 tacttgacgg atcgaagtgc tatggtattt tacatgaaaa taattcgtca atatattttg
    35401 accaatcaga caagccatca aaaaagcgtt gaaataaatg gcctgcaact gagaagaatc
    35461 tttcgatgaa gctgcattga ttacattggc aatagcgatc ggcattaacc aagatggaag
    35521 atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc caataatttc cctgataaag
    35581 atgcagcaat gtctgaaaag catttgcatg atatttttga taacttttct gtattcgttc
    35641 ctccataatc ttctccttaa caatcttggt aaataaactg caagtgttct attgcttcag
    35701 gaacataaat ctcaggagta gcttcaagag tggcgtacat tagaggacgc tcatatttga
    35761 tataatgcag taccgggcgg aaatctaaac agcccttgcc aacaggtacc atgcggatct
    35821 ttccattttc catgatatag tctttgagat gaagcgctgt aatataacca tgtaaattgt
    35881 ccaaggcacc tcggatcact ttttcctgat cagcatgatt ttctttatgc atcaaattcg
    35941 cacaatctaa aatgattttt aaatttggtg aagctacttc atcgattaaa cgtttagcta
    36001 actgatagtt atagattgga tgattgatcc ctgcttcaac agcaacagtt acgccaaaat
    36061 tttctgcatg agcgactaat tcaatgatag aatttctggc gatctgataa gcttcttcag
    36121 taaaattttc ttttgtgtaa ccgacagaga cactaccggt ttccgtagca atcatcttgg
    36181 cttgataggc agagcacaga gcaatttgct gtttgaaagt tgccaatgct tgtgcacgaa
    36241 cttcttgatt tttgctggat aggttgatat agcatcctaa aatggaaagt tcgatattgt
    36301 ttttttggaa gtattcacca aaataggcag cactcccaag tgtcatgtca gttaatttaa
    36361 gttgttcagg aaatgctttt tgtggtgcaa attggatatg ggacaagtga agctgtgata
    36421 ctttttgaac gatttcttca cgatttcgaa catctaaatc gtgggcacgg atgcctaaat
    36481 ttaatggaaa catctcaaac tcctttttgt aaataattag caagaggcag acgggattct
    36541 tttatcaatt caacaattat tcgggcaact ttatcagcgc cttctggact aaagtgggta
    36601 ttgtctttta gtccttctgg gtaattttta caagtatcag gcataagatg caggtagaat
    36661 ttttttgtct cttcttttga gaaggtctgg aacaattctt gtgatcttga gaacacatct
    36721 aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg cttttggata gtctccaagt
    36781 atatctggat tcaatgttcc gttttctaag taatctcgtc gcgtcacaga tgtcagtaag
    36841 accatttgga cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt ttttaaatag
    36901 ctaccgaagg gaagcgtacc tctttcttct tggattttct ggtcgttatg tccaaattgt
    36961 acaaataaga aatcattcgg ctgaaactgt gaagctagac gggctaacct tccttcctgg
    37021 ataaatgatt tggtgctgcg tccattcttt gcttggttat tgatctggat ctcgggtgaa
    37081 aaataagcag agattttttc tccccaacct gtctcgggac gcttgttttc atctttttct
    37141 gccgcagtag aatcaccggc aatatggata gtaggcatgt tatctcttct ttcataaagg
    37201 tttataggca atgaagcaga acagcaagag aagaagacgg agcaa
//
LOCUS       DANJLF010000022        35398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000022 DANJLF010000000
VERSION     DANJLF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            8..649
                     /locus_tag="PWT25_001205"
     CDS             8..649
                     /locus_tag="PWT25_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021302.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="PWT25_001206"
     CDS             708..1148
                     /locus_tag="PWT25_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021303.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="PWT25_001207"
     CDS             1160..1828
                     /locus_tag="PWT25_001207"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL1021304.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="PWT25_001208"
     CDS             1892..2515
                     /locus_tag="PWT25_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL1021305.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="PWT25_001209"
     CDS             2571..2957
                     /locus_tag="PWT25_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL1021306.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="PWT25_001210"
     CDS             3170..4561
                     /locus_tag="PWT25_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL1021307.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="PWT25_001211"
     CDS             5172..5540
                     /locus_tag="PWT25_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL1021308.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="PWT25_001212"
     CDS             5720..6661
                     /locus_tag="PWT25_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL1021309.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="PWT25_001213"
     CDS             6730..7461
                     /locus_tag="PWT25_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL1021310.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="PWT25_001214"
     CDS             7605..9785
                     /locus_tag="PWT25_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL1021311.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="PWT25_001215"
     CDS             9805..10287
                     /locus_tag="PWT25_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL1021312.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="PWT25_001216"
     CDS             10303..11064
                     /locus_tag="PWT25_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL1021313.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="PWT25_001217"
     CDS             11195..12529
                     /locus_tag="PWT25_001217"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL1021314.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="PWT25_001218"
     CDS             12836..13321
                     /locus_tag="PWT25_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL1021315.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="PWT25_001219"
     CDS             13314..13997
                     /locus_tag="PWT25_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL1021316.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="PWT25_001220"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="PWT25_001220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL1021317.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="PWT25_001221"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="PWT25_001221"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL1021318.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="PWT25_001222"
     CDS             17613..18242
                     /locus_tag="PWT25_001222"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL1021319.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="PWT25_001223"
     CDS             18294..18626
                     /locus_tag="PWT25_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL1021320.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="PWT25_001224"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="PWT25_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL1021321.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="PWT25_001225"
     CDS             19452..20825
                     /locus_tag="PWT25_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL1021322.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="PWT25_001226"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="PWT25_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL1021323.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="PWT25_001227"
     CDS             21880..22320
                     /locus_tag="PWT25_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL1021324.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="PWT25_001228"
     CDS             22464..23165
                     /locus_tag="PWT25_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL1021325.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="PWT25_001229"
     CDS             23162..24283
                     /locus_tag="PWT25_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL1021326.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="PWT25_001230"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="PWT25_001230"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL1021327.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="PWT25_001231"
     CDS             25662..26570
                     /locus_tag="PWT25_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL1021328.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="PWT25_001232"
     CDS             26605..26877
                     /locus_tag="PWT25_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL1021329.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="PWT25_001233"
     CDS             26888..27934
                     /locus_tag="PWT25_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL1021330.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28185..30794
                     /gene="clpB"
                     /locus_tag="PWT25_001234"
     CDS             28185..30794
                     /gene="clpB"
                     /locus_tag="PWT25_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL1021331.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30945..31631)
                     /locus_tag="PWT25_001235"
     CDS             complement(30945..31631)
                     /locus_tag="PWT25_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021332.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31609..32103)
                     /locus_tag="PWT25_001236"
     CDS             complement(31609..32103)
                     /locus_tag="PWT25_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL1021333.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32123..33343)
                     /locus_tag="PWT25_001237"
     CDS             complement(32123..33343)
                     /locus_tag="PWT25_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1021334.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33490..35325
                     /gene="lepA"
                     /locus_tag="PWT25_001238"
     CDS             33490..35325
                     /gene="lepA"
                     /locus_tag="PWT25_001238"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL1021335.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        11847 a         6250 c         7612 g         9689 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
    28021 tatacataca atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa
    28081 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
    28141 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
    28201 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
    28261 atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc
    28321 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
    28381 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
    28441 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
    28501 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
    28561 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
    28621 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
    28681 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
    28741 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
    28801 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
    28861 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
    28921 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
    28981 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
    29041 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
    29101 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
    29161 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
    29221 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
    29281 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
    29341 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
    29401 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
    29461 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
    29521 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
    29581 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
    29641 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
    29701 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
    29761 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
    29821 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
    29881 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
    29941 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
    30001 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
    30061 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
    30121 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
    30181 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
    30241 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
    30301 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
    30361 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
    30421 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
    30481 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
    30541 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
    30601 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
    30661 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
    30721 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
    30781 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
    30841 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
    30901 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
    30961 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
    31021 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
    31081 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
    31141 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
    31201 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
    31261 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
    31321 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
    31381 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
    31441 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
    31501 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
    31561 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
    31621 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
    31681 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
    31741 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
    31801 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
    31861 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
    31921 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
    31981 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
    32041 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
    32101 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
    32161 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
    32221 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
    32281 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
    32341 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
    32401 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
    32461 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
    32521 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
    32581 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
    32641 cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
    32701 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
    32761 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
    32821 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
    32881 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
    32941 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
    33001 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
    33061 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
    33121 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
    33181 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
    33241 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
    33301 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
    33361 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
    33421 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
    33481 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
    33541 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
    33601 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
    33661 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
    33721 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
    33781 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
    33841 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
    33901 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
    33961 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
    34021 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
    34081 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
    34141 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
    34201 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
    34261 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
    34321 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
    34381 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
    34441 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
    34501 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
    34561 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
    34621 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
    34681 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
    34741 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
    34801 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
    34861 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
    34921 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
    34981 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
    35041 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
    35101 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
    35161 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
    35221 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
    35281 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
    35341 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtg
//
LOCUS       DANJLF010000023        34330 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000023 DANJLF010000000
VERSION     DANJLF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            202..1077
                     /locus_tag="PWT25_001239"
     CDS             202..1077
                     /locus_tag="PWT25_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021336.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="PWT25_001240"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="PWT25_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="PWT25_001241"
     CDS             complement(1642..2973)
                     /locus_tag="PWT25_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1021337.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="PWT25_001242"
     CDS             complement(3122..3835)
                     /locus_tag="PWT25_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1021338.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="PWT25_001243"
     CDS             4010..5434
                     /locus_tag="PWT25_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1021339.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="PWT25_001244"
     CDS             5733..6440
                     /locus_tag="PWT25_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL1021340.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="PWT25_001245"
     CDS             6427..6750
                     /locus_tag="PWT25_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL1021341.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="PWT25_001246"
     CDS             complement(6839..7876)
                     /locus_tag="PWT25_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1021342.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="PWT25_001247"
     CDS             complement(8197..9180)
                     /locus_tag="PWT25_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL1021343.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="PWT25_001248"
     CDS             complement(9595..10365)
                     /locus_tag="PWT25_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021344.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="PWT25_001249"
     CDS             complement(10358..11293)
                     /locus_tag="PWT25_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1021345.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="PWT25_001250"
     CDS             complement(11609..13258)
                     /locus_tag="PWT25_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL1021346.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="PWT25_001251"
     CDS             complement(13375..15978)
                     /locus_tag="PWT25_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL1021347.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="PWT25_001252"
     CDS             complement(15990..17039)
                     /locus_tag="PWT25_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL1021348.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="PWT25_001253"
     CDS             complement(17065..17379)
                     /locus_tag="PWT25_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL1021349.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="PWT25_001254"
     CDS             complement(17395..17841)
                     /locus_tag="PWT25_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1021350.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="PWT25_001255"
     CDS             complement(17842..19764)
                     /locus_tag="PWT25_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL1021351.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="PWT25_001256"
     CDS             complement(19976..20872)
                     /locus_tag="PWT25_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1021352.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="PWT25_001257"
     CDS             complement(20865..21545)
                     /locus_tag="PWT25_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1021353.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="PWT25_001258"
     CDS             complement(21546..22328)
                     /locus_tag="PWT25_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021354.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="PWT25_001259"
     CDS             complement(22321..23238)
                     /locus_tag="PWT25_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1021355.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="PWT25_001260"
     CDS             complement(23402..23725)
                     /locus_tag="PWT25_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021356.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="PWT25_001261"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="PWT25_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL1021357.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="PWT25_001262"
     CDS             27853..28167
                     /locus_tag="PWT25_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL1021358.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="PWT25_001263"
     CDS             28180..28554
                     /locus_tag="PWT25_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL1021359.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="PWT25_001264"
     CDS             28564..28899
                     /locus_tag="PWT25_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL1021360.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="PWT25_001265"
     CDS             28982..30322
                     /locus_tag="PWT25_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL1021361.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="PWT25_001266"
     CDS             30400..31074
                     /locus_tag="PWT25_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1021362.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="PWT25_001267"
     CDS             31067..31231
                     /locus_tag="PWT25_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021363.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="PWT25_001268"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="PWT25_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="PWT25_001269"
     CDS             32439..32729
                     /locus_tag="PWT25_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021364.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="PWT25_001270"
     CDS             32988..34172
                     /locus_tag="PWT25_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL1021365.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34169..34306
                     /locus_tag="PWT25_001271"
     CDS             34169..34306
                     /locus_tag="PWT25_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL1021366.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DANJLF010000024        33746 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000024 DANJLF010000000
VERSION     DANJLF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            119..2446
                     /locus_tag="PWT25_001272"
     CDS             119..2446
                     /locus_tag="PWT25_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1021367.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2572..2901
                     /locus_tag="PWT25_001273"
     CDS             2572..2901
                     /locus_tag="PWT25_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL1021368.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2941..3945
                     /locus_tag="PWT25_001274"
     CDS             2941..3945
                     /locus_tag="PWT25_001274"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL1021369.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3942..4388
                     /locus_tag="PWT25_001275"
     CDS             3942..4388
                     /locus_tag="PWT25_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL1021370.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4538..5353
                     /locus_tag="PWT25_001276"
     CDS             4538..5353
                     /locus_tag="PWT25_001276"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL1021371.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5559..6731
                     /locus_tag="PWT25_001277"
     CDS             5559..6731
                     /locus_tag="PWT25_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1021372.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6893..7387)
                     /locus_tag="PWT25_001278"
     CDS             complement(6893..7387)
                     /locus_tag="PWT25_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021373.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            7970..8392
                     /locus_tag="PWT25_001279"
     CDS             7970..8392
                     /locus_tag="PWT25_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021374.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8558..9751)
                     /locus_tag="PWT25_001280"
     CDS             complement(8558..9751)
                     /locus_tag="PWT25_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1021375.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9975..10352
                     /locus_tag="PWT25_001281"
     CDS             9975..10352
                     /locus_tag="PWT25_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1021376.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10340..10678
                     /locus_tag="PWT25_001282"
                     /pseudo
     CDS             10340..10678
                     /locus_tag="PWT25_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10812..11255
                     /locus_tag="PWT25_001283"
     CDS             10812..11255
                     /locus_tag="PWT25_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL1021377.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11516..12331
                     /locus_tag="PWT25_001284"
     CDS             11516..12331
                     /locus_tag="PWT25_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1021378.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12341..13003
                     /locus_tag="PWT25_001285"
     CDS             12341..13003
                     /locus_tag="PWT25_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL1021379.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13211..14875
                     /gene="alsS"
                     /locus_tag="PWT25_001286"
     CDS             13211..14875
                     /gene="alsS"
                     /locus_tag="PWT25_001286"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL1021380.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14888..15598
                     /gene="budA"
                     /locus_tag="PWT25_001287"
     CDS             14888..15598
                     /gene="budA"
                     /locus_tag="PWT25_001287"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL1021381.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15728..16225
                     /locus_tag="PWT25_001288"
     CDS             15728..16225
                     /locus_tag="PWT25_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL1021382.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16410..16670
                     /locus_tag="PWT25_001289"
     CDS             16410..16670
                     /locus_tag="PWT25_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021383.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(17032..17271)
                     /locus_tag="PWT25_001290"
     CDS             complement(17032..17271)
                     /locus_tag="PWT25_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021384.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            17557..17871
                     /locus_tag="PWT25_001291"
     CDS             17557..17871
                     /locus_tag="PWT25_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021385.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(18305..18671)
                     /gene="ssrA"
                     /locus_tag="PWT25_001292"
     tmRNA           complement(18305..18671)
                     /gene="ssrA"
                     /locus_tag="PWT25_001292"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(18842..19615)
                     /locus_tag="PWT25_001293"
     CDS             complement(18842..19615)
                     /locus_tag="PWT25_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1021386.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            19759..20109
                     /locus_tag="PWT25_001294"
     CDS             19759..20109
                     /locus_tag="PWT25_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL1021387.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            20090..20806
                     /locus_tag="PWT25_001295"
     CDS             20090..20806
                     /locus_tag="PWT25_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1021388.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            20806..22254
                     /locus_tag="PWT25_001296"
     CDS             20806..22254
                     /locus_tag="PWT25_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1021389.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(22324..22833)
                     /locus_tag="PWT25_001297"
     CDS             complement(22324..22833)
                     /locus_tag="PWT25_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021390.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(22823..23548)
                     /locus_tag="PWT25_001298"
     CDS             complement(22823..23548)
                     /locus_tag="PWT25_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021391.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(23771..25891)
                     /locus_tag="PWT25_001299"
     CDS             complement(23771..25891)
                     /locus_tag="PWT25_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL1021392.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            26121..27299
                     /locus_tag="PWT25_001300"
     CDS             26121..27299
                     /locus_tag="PWT25_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL1021393.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            27315..28073
                     /gene="aroD"
                     /locus_tag="PWT25_001301"
     CDS             27315..28073
                     /gene="aroD"
                     /locus_tag="PWT25_001301"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL1021394.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            28096..28581
                     /gene="ybaK"
                     /locus_tag="PWT25_001302"
     CDS             28096..28581
                     /gene="ybaK"
                     /locus_tag="PWT25_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL1021395.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(28652..29905)
                     /locus_tag="PWT25_001303"
     CDS             complement(28652..29905)
                     /locus_tag="PWT25_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1021396.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            30023..31372
                     /gene="gdhA"
                     /locus_tag="PWT25_001304"
     CDS             30023..31372
                     /gene="gdhA"
                     /locus_tag="PWT25_001304"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL1021397.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            31484..32830
                     /locus_tag="PWT25_001305"
     CDS             31484..32830
                     /locus_tag="PWT25_001305"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL1021398.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(33138..33524)
                     /locus_tag="PWT25_001306"
     CDS             complement(33138..33524)
                     /locus_tag="PWT25_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1021399.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(33573..>33746)
                     /locus_tag="PWT25_001307"
     CDS             complement(33573..>33746)
                     /locus_tag="PWT25_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL1021400.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT        11237 a         5933 c         6709 g         9867 t
ORIGIN      
        1 accttagaat aaaaagagaa aaaaagcccc aaactttcta tacatcaata aaataataaa
       61 aattaaattt agaataacgt gtaaaggatt atttgatgat tttttattga gaggagggat
      121 gaaaatgatt aagaaaactc aaaataaaat ccaacgtata tgtcaaaaac attttgtttc
      181 tagactcaaa gctgtatcta aaagaaaata cttacatccc attatgattt cgatcatctt
      241 gatttttact ttcttaggga gcttatcaag tatttatgta aacgtttata cagaaacgtc
      301 aaattttgct aaccaaacga cagaggagac aaatagtcca ccctattctg aaatacagtc
      361 catagaaagc accaatcagt ctagcaacaa cgagacaacc tcaaccacat cctcgactca
      421 actgattgat ccgacagaaa aagaaatcac agcagcaaaa gcagcagggg agaaagctta
      481 ttcagaaaca ggtagaagcc agacatttag tactgttgcg gccactgcga atttcaacgg
      541 acataccata ccagatggat atacagtgat atatgccaaa gatggagtca ttgttgcggt
      601 taacggatcg atgagtgcgg ataaaactgc gttacaaggc gtaaccaaag caatcgcttt
      661 tattgataac cgtccagata tccataatct gtttattggc tccacagtaa atggtactac
      721 tatcacattc ccaaaaagta tctatatggg ggtggctacc gctggaaata cgatcgattc
      781 agttacaccc aatgcggata aaagttattc ttttacaccc tcaacatggg gagggcttcc
      841 tgccggagga ggtatgagta ctttttcaat cggtatacag ttagcaaatg atattccatt
      901 cacggggtac tcgatcaaaa atgggcagat cgttgatggt aagggaaatg tttcaaattt
      961 tccaacctat caagcagcaa cggttcaggt caatgcgaat cggacaggaa ctttttctta
     1021 tacagtcaat ggtggagcag caaaaatcgg aacgtacggg actacattat ctggaatcca
     1081 tgtgggtgac aaagtggtta tcactccaac agcagtaacg ggctattctt ttgcacaaac
     1141 aaacggttct ttcacagccc tagaaagtaa cgtcgatacg gtgacctata caggcaaaca
     1201 aacaccaatc actattaagt atcaagacta ttttggacag atgattgctc cttctaaaat
     1261 aatgaatctt acttacggaa gtgctgcaca agacctgacg actaatacgc ctactatcaa
     1321 cggatatacc tttacagctg tttcagcgat agaaacaaag aaccaatcag caatgagtgt
     1381 tttagctagt ttagatacaa atggaaatgt aatcgtaaag gacgcaaatg gaaaacagat
     1441 ttctgaagtc atcctatatt ataagacaaa tgcaacagta tcaatcaatg caaatggcag
     1501 taagtactat gatggtcttt ctgttgtacc gatcgttaaa tatgccttta atgataaaac
     1561 agaatcaaca tcccttttaa acaatgatct cagtattgat caaatcacat ggaatccaac
     1621 tgatttcaaa gcgatgaatg aaaagggagt tgaagtaact gcgcctactg aaattggaac
     1681 ctatacgtct tggcaattga caaaaagcgg actagctaaa ctagctgcca gaacaaacta
     1741 tttatttact atcgtgcaga catctgatgt ctatacgatt aagcaaatca gcggtgcggt
     1801 agagctcagt ggcagtaaga catatgatgg aaaagcaggt gtcccgtcaa tccatgtcaa
     1861 gctagcagag ggtgtcacta gttcgcttac tccagtacaa gtggcactta gcagtatgga
     1921 ttacacagca gatgcacaat cagcaaaaaa tatggttaat gctggcagct atacaattaa
     1981 actaacgaat agcggaatag acaaagtcaa agcagcaaat cccaagttgt catttactga
     2041 tatagccaac acatcaggaa cttataccat ctataaagct aacgcagtga ttactgtcga
     2101 tgacgctgcc tttaattatg atgcccaaag tcactcgatt tcggttggaa atctccacgt
     2161 taccggtgtt gtcttaggcg aatcattgga ttatacccta acagttaaca gccggacaga
     2221 tattggatca caaatagtaa cgattagttt aggcgaagga agtgtaaaca ataattatac
     2281 gattatcgca acagataccg ctaagttgac gatcaatccg actctcttgc ttccttcgac
     2341 aggtgggata ggtctactgc cctttgtctt tttaggactg atctttattt tttctggctt
     2401 cttttatttt atccatcgta agaaaaaagc aggtgaacag cgatgaaaaa aacaaaaaat
     2461 gtacgtgtgg atatatttgc tgttctcttt tttatcctac tggtcttttt tataggttct
     2521 ccgactgtgt cagctagaga atcacctatt acatttactt tgcataatcg aatgttcgaa
     2581 agcgataaga tgccaagtat cagtcaaaac gacggaataa atatcccgga taactttcaa
     2641 ggagtaaaag ggttgaacgg cgttactttc gctatatatg atgtctcgga tgaattttac
     2701 aagctgcggt cagaaggaag tagtgtggaa gacgcacaaa gaaaactagc acaaaaaagt
     2761 gatagtgaaa aaatactggc agaaaatgtg acaaaaacag tagacgagga agagggcatt
     2821 gcttctttta gtgcatcgga caaagatgag caaggtcgtg acgctgttta ccgatttacg
     2881 gaaataaaga cttcagatta agtaaaagaa aaatcagcac catttgtcgt tgtgcttcct
     2941 gtgacagatt catctaatca taaattgacg accatccact tgtatccaaa gagcgaacaa
     3001 aaaatacctg ctgcactcac attaacaaaa acagtagaga ataagcagac agattttgca
     3061 gatggggata aagtccctta tctcatcacc gtaacaattc ctgagaacat caacgagata
     3121 ggaacctata cgatcaaaga tacagctgat cctcagttat ggctagaacc agaaacgatc
     3181 gattttttga ttggtggaga caagattcat acatttcaca cacaaaaaat aaaacatggt
     3241 tttgtcttat cagattctgg aaaagatctt agttcgtttg ctggaaaaaa attaacgatc
     3301 cgttaccaaa tgacattgaa agaaaacagt gaaaaaacga catttgataa cgatgtacgt
     3361 ttgacgactg acaatcaaac agtagaaaca catcttgcca tccaaacagg aggcaaacaa
     3421 tttgtcaaag tggatcggca aacgaaacaa aaactagcag atgctcaatt tctagtcaaa
     3481 aaaaatgacc aatacttagc gcaagaaaac gggataaatc attgggtagc caaagatgat
     3541 tcaagagcaa cgatttttac ttccagtaaa gatggaacat tttctgtcca tggattgtct
     3601 tttggaagtt acgaactagt ggagattaaa ccgcctaaag gatatatatt gagtcaatca
     3661 accattctct ttgaagttga aaaaggcagt tatgatccgc ttcatcctat accattggaa
     3721 atcatcaacg aaccaaaagc aaatccgacg aataatggaa aaacgccagt tcagatgcag
     3781 acaaatggtc catcaaatga aaaaaatgga ggaagctttc caaaaacaag cgaagagatg
     3841 cttggaggtt tctcaatttt agggttattg ctagtccttt caacagggac agcttggttt
     3901 tataaaaaac aacaaaaagg aaacaatagg agggaaataa aatgaaaaac atcaaaacaa
     3961 aaatatggac gtttttagga actgctatca tgctgcttcc tttcgttctc ggcctaggaa
     4021 ctgcagaggt ttctgcagcc gtatcaccta caccagaaaa tgttacggtt aatttgcata
     4081 aattgaaatt cacttcagca ccagaaaatc aaataaacaa cggaacggaa ttgacatttc
     4141 cgaatagcga accgttgaac ggcgttgaat tcaacgtata tgacatcact gctacgtact
     4201 atccaagtaa agacacagca gttccagcag acgctacacc cttcgcaagt gtaacgactt
     4261 ctggtgaagg attagctaac ctgactcttc caggtaaatc tgatgggaaa gatgctgtat
     4321 acgtctttgt agaaacacca aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct
     4381 taccctagac cctgacaaac ctttagacac gattcaccta tatgctaaaa atgtcatcaa
     4441 agaaagcaat atcaaaatcc agaaagctgg gaacttttca ggggtagata agttagcagg
     4501 ggcgcaattc cagatcaaaa attcagagaa tcaattcgtg accggttttg atgaaaacac
     4561 tggtgtcgct ctctatgatc ctgctgagtc agcgttgaca ttcacaactg atactgaagg
     4621 atccatcagt gtcaatggat taatagacgg caattatact ttagttgaaa caaaagcacc
     4681 agatggttat caattaacca atccacaaaa agaaactact tttatagtaa aaaacggaaa
     4741 attgcaacaa acaaccggta tcatccatgg cgcagatgaa gccaatcttg ggaatccaaa
     4801 cggtgtagtc aatcgggaaa atacaactac ggataacacg attactgtag aaaatgtcca
     4861 aaactacggt gatttccact tcatcaaaca agatgtgaac acgcaagaaa aattagccga
     4921 tgcagaattt tctattctaa gagcaaaaga tccaactgtt aaattaatga aaaaaatagc
     4981 aaaccttgcc tcaggagaat acccatatgc ttggtcagat gaagtcacag gttctgactg
     5041 ggaagaagtg aaattgattt ctgattcaga agggaaattt tcaatcgaag ggctgaaaca
     5101 aggggcatac tgccttcaag aaacaaaagc accagaaggc tacgcattac ctaagaatga
     5161 agctgcgtac actgaattta cagtagatga tgatccaaca acttctgatg aaacaacgat
     5221 cgataaccaa cctaaaggaa tcttgccttc aactggtggt atggggatca tcgtattcat
     5281 cttggtaggt actgctttag ttggaggagc agtgatctat ttcaaaaaac gccatcaaga
     5341 aacagaagct taaactattg gaaacaaaaa tcatacgata aataaacgat agtgattcaa
     5401 atggggagaa aggttgtgac aagtggtcgg tctcttggta ttcgctcaac aaacggcaaa
     5461 ataggaggaa ctattttgcc gtttgcttcg ctaatactca ggtcaaggcg ctgatttcac
     5521 aacctcttca tatacatatt agaaaatagg tggacatgat gaataaaagc agaataagaa
     5581 tactggacat cttcatggca atcattttag tcgttgggat cgggatcttt tcttatcctt
     5641 ttgtagaaga ttcactgaat gattttcttg cccagcaaat gatcatccat tatcaaaagc
     5701 aagcatcgaa taagaattca gaggaaataa aaaaacagca ggaaaaaatg acaaagaaaa
     5761 accaacagtt ggcagagcaa aacgtatcac cagggatagg cagtttcaac caagcagttg
     5821 atgcaaaagc attaaaagat ttacctagta atgccttttt tatggaacat atgcttggag
     5881 tcatcgaaat cccaaaaatc aatgtcagct tacctatatt cgatcagaca acggaaatat
     5941 ttttgcaaaa aggaacgtca ttattggaag gaagttctta tccgactggc gggaaaaaca
     6001 cccatgccgt tctttctggg catcgtggac tcccagaagc aaaattattt acagatttgc
     6061 ctaagttgaa aaaaggagac cagtttttca tccagatcaa tggaaagaca ttggcgtatc
     6121 aagtagaaaa aatccaagtg gtcttacctg acgaagtaga ttctttgggt atccaaaaag
     6181 gcagagatct cgtgactttg cttacctgca ctccttatat ggtcaataca catcgactgc
     6241 ttgtgacggg gcatcgtatt ccttatcaag caaaagaggc aaaaaaagcg atccaaggaa
     6301 ttgatcaatg gaaaaaatgg aaatttttta tttggtttat cggtatacta cttggatcaa
     6361 tcggcttagt ttggctactc atcgcttatt tagattcttt ggccatcgct aagcgaaact
     6421 atcctttatc tttttatgta aagaacacaa acggacgacc aatcgaagga atggtctttt
     6481 ctgtaaaaac gctcaacggc aaacactata ttactagaga aaaagttcca tttgtgaaag
     6541 cttctgatga atacggacta gtgagatttt ctgatttaaa aggcaggaac tatcgcttgc
     6601 aacatgagga gctcctgctt aaaatccatg taaaacataa acattcgaaa cagttttcaa
     6661 tgaaattgaa aaaaggaaga tataaactgc gaaaagaaaa agaggtgtac tatttgattg
     6721 agaaagaatg aatgaagatg tccaaaaaag cagagggaac agactaaatg aaaaaagaga
     6781 gaagctgacg aaatggacgt gtaaactgct atttttggtt tactatctgg aacgccagct
     6841 tctatttttt tatgtttcag aagaatttta ttattatagt ataacttttg aattagtttc
     6901 cataacattt ggagcaaggg gttaaaccac gagcttgagc ttcgcttaaa gtggcagggg
     6961 tatacgtacc atttccacat ttatgtgtat ggtattttga accggtcggt gtgacataga
     7021 cagtttgttc attattttgc tcaggagctg cggcttgtgc ctgctgagaa gcctgttcag
     7081 ctgctgcttg ctgtcgcgcc tgttctgctt gttgctgtgc gattgcttct tgcctcgcct
     7141 gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg ttctgcttgt tgtcgtgcaa
     7201 tttcttcttg ctttttccgt tcttcttctg cttcttttgc tttgattgtg gaatcaattg
     7261 cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag cgcttggata gctgctgaag
     7321 cagttgcata gttttctttt gtaggatttg cttcagcttg tgacaaagca gattccgctt
     7381 ctgtcacttt tttttctagt gcagcttttt ccgctttttg tttctcttct tcttttttct
     7441 tttcaatttg ttctttcgat gaatctgccg tactttcgga gatcgtggta ctgctgctgg
     7501 aagcctgagc tgaacttgtg ctttgagtag tagacgtagt agcaccacac ccactcaaca
     7561 aacttcctgc gaccaaaaac aacgcaacta attttttcac taaatttcct ccttaagtaa
     7621 aaaacaaccg ttttcaataa atgtcctttt caattgttta cttacttgtt ataataattg
     7681 tgttatgaat agtgattgag gaaacttaat ctaatcccgt gttgatacac gggatttttt
     7741 gctatagttc tgttattaag tatgcaataa taaaatatgt acgttatttt attattagat
     7801 tattgacatt tatataataa acgattaaat gattcatatc aatagaatac ttaggaaaaa
     7861 aagagaaata ataacaggtt tatgcactat tgacggtgtg acaatatgat atttgtatcc
     7921 gtttttttct tgtatgtagt atagtaaagt agatacagga ggagaaaata tgcagacttt
     7981 tttaacaaaa gtttctcaaa agaaaattaa aatgattcat ttactgcttg aaacagaacg
     8041 ttggtgttca atagaagaac tacaaaacga attgaaggta tcaagtaagt ctatcttgaa
     8101 ttatctaaca gatttagaag aattgttcca acaatatcct gataaggtca ttttgaagaa
     8161 tgagaataat cggcgctttt ctatggaaaa gcaggagaac ttcccaatct acattattta
     8221 tcttcatttc tacagaaaat catataatta tcatttgatt gaattcatgt atcaacatcc
     8281 tgagaaaaat ttggaagatt atgcagacgc tcaatatacg agtgtatcca cagtttttcg
     8341 ttatgctaag ttattagtaa agtactttga aatgacggac gtcaaagaat agtcgccgcc
     8401 agatagaagg aaattagcag ttgaaaaagc tgccctgcat caagtaatac gaccagccaa
     8461 acggaaacag tgtctcatgt ttttgatttg gctggtctta ttaccgcaga tgcaagcttt
     8521 ttccacacct ctttcttgtc atttacagac tcaactatca atgctgcaag ctttccctta
     8581 atgctgtaac accagtaaga gttgtcaaga ccagcagact gactaggtaa ataccgctca
     8641 aaaacaagat tacggcagtt aaactataat gatccatcaa gaaaccgatg acgatagaag
     8701 aaaaaccacc aacagctcgt ccaaggttca taacgaaatt attcgccgtc gcgcggattt
     8761 ctgttggata caaactacta atcactgcgc catatccacc atacatgcca ttgataaagt
     8821 agccgactat cacagcggct gctaccaaac tccactggct atgggctaac acaagtagat
     8881 agacagataa ggatgaacaa attaaaaaca aagaaaaggc tagtttaggc cccaatttat
     8941 ccatgattat gccaaaagtc atcatcccca aagacatgcc taaaatagta ctgaccatcc
     9001 acaatgaaga cccagaaacc gaaagcccta atcgattttg cataattgaa ggtagccaat
     9061 tcattaaccc aaaatagccg gcaatttgga cagttaccat gatactaagg ccacatgttt
     9121 gccaagcaag atgttttgat tcaaacagtc tgctaagttt ggttttcttt tcggcagatt
     9181 gtacagattt gaattccttt ggttcagtca agccgcgacg aatgaacaag accatcaaca
     9241 ccggcagtac acctacgaca tacaacatct tccaaccaaa cgttggaatg atcacagcag
     9301 ctaaaatcgc agccaaagcc gcaccgacct gcgcaccaat accagcaacc gaagaagctc
     9361 gtccaatttg tttttttgaa aaactttcgg aaatcaagga catacacgcc ccatattctc
     9421 ctcccgctcc tacacctgcg ataaagcgaa agagataaac caagtagatg tttgaagcaa
     9481 aatacatcaa taaagaagca atcgaaaaca gaaggaccgt ttgagaaaat actttcacac
     9541 gtccatattt atcagctatg atgccaaaaa aaatacctcc aatgagcatc cccaaattag
     9601 tgatcgttga aatgaaaccc gcttgcgtac cactaatatg aaaagaagta atcattgaag
     9661 ataatgaaaa agctaaaaac ataatatcca tattctctaa agcaattccc gccgatgtcg
     9721 atactaatac ctttcgctga tacgttgtca tttttatacc ccttttccta aaatttccct
     9781 aaattgttaa aagtatatcc aaaaaagaat aaaaaaacaa gactttttta tttgaaataa
     9841 tacacaaaca accgatatag ataatccagc ttagttttaa tagaaatgag tattgacttg
     9901 gagtacactt aaagttgtag aataaataaa atacataagt aaagtggcaa gaaattaaga
     9961 gaaagagtga tcagatgaat atcaaacaag tcagtgaaga aaagggcatt tcggccgata
    10021 cattgcgcta ttatgaacga atcgggttga ttccaccagt taatcggaca aaaggcggaa
    10081 tccgtgatta tacagaagaa gacctaaaat gggtcgactt tacgatctgt atgcgaaatg
    10141 ccggattatc gatcgagtct ttgattgaat atattcgcct atctagcgaa ggagaagcaa
    10201 ctgtatttga gcggcgtaag cttttgattg aagaaagtga acttttgacg aaaaaaattg
    10261 ctgaaatgca agaatgtttg gaacgtttac aaggaaaaat caagaaatat gatcgtgtgt
    10321 tatctagaaa taaaatggaa tgtatcaagt aaaaaaatca aggagcatcg ctatggagtt
    10381 acgcttacta aaatattttt ggacaattgc agaagaagga acaatctctc aagccgcaga
    10441 agttctacat cttacacaac caaccttaag ccgacaactg aaggagttag aagagtaact
    10501 aggaacagaa ctgtttgtac gcgaaaaccg caaaatgatc ttgacagaag caggttattt
    10561 tctaaaaaag ccgagcagaa gaaattttag atttaacgaa tcaaaccact caagaattcg
    10621 agaacagaaa aaaacaatta ttcagcggcc gatttacgat cggctgtgtg gaagctgata
    10681 gttcgaatac attggcacta ttactagaaa agttgatccg tgattatccc agcgttcaat
    10741 ttcatatcca cagcggaaca agtgatgata tcacggagtg actagaaaaa agcctgttag
    10801 atttagcgat tttgctagat ccagtagata cagagaaata cgaaacgatt ttgttaccaa
    10861 gaaaggaaaa atggggattg ctcattttag aaaattctgt tttagcaaaa aaagacgtga
    10921 taacaaaaga agactttgcg aatgtacctc ttttgatctc aaagcgtcca gaaatacagc
    10981 gaatgtttgc tgactggtta ggcataaaaa aggagcagtt gctgatcaca ggaacgtaca
    11041 atttgatttt caatgtattt gcgctaattg agaatcaggt aggtgctgct ttagtcatag
    11101 aaggtgctgt aagtcatcat tcaaagcgag tgacattcgt tccgcttgac ccaatgattg
    11161 aaacgaactg catgctagtc tggaaaaaga atcggatttt atcacctgta gtcaatgaat
    11221 tgattcaccg tttcaaagaa gcttttgaac agtaataagt atctctaatc gaagaaattt
    11281 caatacaaaa gcgagacgta tgacagtctt gcttttgtat tttttcattt ttaaatcaaa
    11341 aaaataaaga gatttacata acttgttaaa taagcgctta caaaaataag aagaacggaa
    11401 atgaatcagc tataataacc gttgttctgg attgtaatca ttacaaagaa atgaagatta
    11461 taactttgga ggtgttcgaa atgaaagaaa aaaagcaaaa attcgttcaa aaaacatgcc
    11521 ctttattcaa tggttgggta tgattctgct gattgtatta gctttattga cgatcattcc
    11581 ttttttatgg atggtttctg cttcattcaa agcaaataat gaagtgttca cgattccgat
    11641 tcaatggatc cctaaaactt ggcatttaga gaattatctg gttatttggt ctcgtattcc
    11701 tttagtgaca ttttttaaga atacagcttt tttaagcgtt gtgatcacgt tgattcagtt
    11761 gtttacttct agttttgctg cttacgggtt ttctaaacta aattttaaag ggcgcgatat
    11821 tttattttta gcatatatcg gcacgattgc tgtcccttgg cagtcttata tgatcccgca
    11881 gttcattatg atgagaaaag tgggtttaac agatacgttg tggtcgttgg tgctattaca
    11941 agcgttcaat gcttttggtg tgttcttgtt gaagcaatat tacagtagta tcccagattc
    12001 tttatgtgag agtgcgcgga tcgatggctt atcagagtgg ggaatctatc gcaagatcat
    12061 tttacctctt acaaaaccgg cgattgccag tttgacgatc atcacattcg ttaatacatg
    12121 gaacgattat atggggccat tcatttattt gtcttctacg gaaaataaaa caatccagtt
    12181 agggttgaaa atgtttattg ggctatttga tgcagagtac gcattaatca tggctgcctc
    12241 agttgtttct gttcttcctg ttgcttttat atttttagcg atgcaaaaat attttgtaga
    12301 agggatcgct tcatcaggaa tgaaggaata ggaggtgatt atgaaagagc agcgttttga
    12361 caaaaatgtt tatatggaat tcttttattg gttgtatcaa tcattggcaa gtagcctttg
    12421 tttttggtgt gtcaatatct tgtatatagc agctgcgttc cttttgtcga tagatggaag
    12481 gaatgtcttt tcctttattt tttctttact ttttattggt ccaggttgca tcgtgatgat
    12541 aggtatgaca gaagaattag caaaagaaaa tagtttttca ccagtacgtt tgttcataaa
    12601 gatgtttcgc caattttggc taaagggatt tttttattgg ctatttgctt ggatagttag
    12661 tgtgataatg atttttgatt gtttcttttt tatacgtttt tcttatggaa aatggttgat
    12721 tccgttgttt gttttacttg cttgtttatc agtttccttt agcaccaact gctggtattt
    12781 tcaggtgaga aatccagcgt cgaaaccaaa tcaagttcta cggatagcgt tctactatac
    12841 gttgaaaaaa tggtatgtga gtttactaga ttttttacta ttgacaagtt tgtttctctt
    12901 ctttttcgta aagccccaat ggtgtattct actaggtcct tctatcgtat ttggtttgat
    12961 ttacttcaac aatcgtaaat tgatgagaac tatggatctg tagactttat aaattgaaac
    13021 tttcaattaa gaatgacagt tttttttatg taaaaactgt cattcttttt cattttttga
    13081 aacatatggc taaacaaatg aaataaaacg tgctatactt ttgtatgaaa caggtttaga
    13141 aactgtatca cttatttgtg attatttcta actaataatt aaaaataaag cataaaaagg
    13201 acgatgttca atggctgaaa aaatgaaaca aggttcgaaa tctgtagtag aaagtttgat
    13261 taatcatcat gttgattatg tattcggtat accaggtgca aaaatcgatg gtgtatttaa
    13321 tgaattagag gatcaaggtc ctgaattaat cgtgactcgt cacgaacaaa atgctgcatt
    13381 tatggcacaa gcaatcggac gtattacagg agagccagga gtagttatcg caacaagtgg
    13441 tcctggtgct agtaacttag ctacaggttt ggtaacagcg acagcagaag gtgacccagt
    13501 tttagcaatt gccggacaag taaaacgaag cgatctattg aagttgaccc atcaaagcat
    13561 ggataatgca gcactgttcc agccaatcac aaaatacagt gcagaaatcc aagatccaga
    13621 aacaatttca gaagtgattg ccaatgctta tcgtatggct aagagttcaa aaaaaggcgc
    13681 gagctttatc agtatcccgc aagatgtagt ggatgcacct gtccaaggta atgtcatcaa
    13741 gccattgagc gatcctaaat taggttctgc ttctgcagat gatatccgtt acctagctga
    13801 acgcattaga gaagcaaaac taccggtact attagtaggt atgcgaggat caagtgaaaa
    13861 agaaacgtta gccatcaggc aattagttga aaagacagca ttgcctgttg tagagacatt
    13921 ccaagcagct ggagtgatct cacgtgaatt agaagcgcat ttctttggtc gtgtgggttt
    13981 atttagaaac caaccaggag atatgctttt gaaacgaagt gatctagtta ttgcaattgg
    14041 ctatgatcca atcgaatatg aagcaagaaa ctggaatgcg gaaaaagatg cgcggatcat
    14101 tgtgatcgat gaagcgccag ctgaaatcga tccttttatg cagcctgaaa gagaattgat
    14161 cggtgatatc tcagccactc ttgatttatt gacaggttca ttagagcctc aacaagtgtc
    14221 agaagatgca aaagaatatt tagcatctct tcaagcaaaa ttaacggagc gggacattgt
    14281 tcaatcaaaa ggagaagcag gtatcttgca tcctttagaa gttattaaca cgcttcagtc
    14341 aaaagtaaca gatgatatga ctgttaccgt agatgtaggt agtcattata tttggatggc
    14401 acgccacttt agaagttatg aacctcgtca tttgttattc agtaatggta tgcaaacgct
    14461 tggtgttgca ttgccatggg cgatttctgc tgcattggtg cgtccaaata cacaaatcgt
    14521 atcagtttcc ggcgatgggg gatttttatt ctctgcccag gatttagaaa cggcggtacg
    14581 taaaaaattg aatatcgttc atttgatttg gaatgatgga cactacaata tggtagagtt
    14641 ccaagaaaaa atgaaatatc agcgggcgtc aggtgtagac tttggtccag ttgattttgt
    14701 gaaatatgca gaagcttttg gcgctaaagg aatccgagct acaagtgtag aggaattaga
    14761 aaaagcttta gaagaaggat ttgcgacaga aggacccgta attattgata ttccgattga
    14821 ttaccgtgat aatgagaaat tgggagaaac aatcttacca gatcaattct attaaggaag
    14881 tgtcgacatg actgaaaaaa tcttatacca gcacgggaca ttaggagctt tgatggccgg
    14941 cttgatggat ggaacagaaa cgattgctca tatattagaa aaaggaactc tgggattggg
    15001 aacattgcat gggcttgatg gtgaagtcat ctttttagat ggcgtagctt atcaaggaag
    15061 atcagatggc tcagttgccc aattagatgg aagtgaactt accccctatg cagcaattac
    15121 agattttacg ccagatacta gtttttctgt ctcaaaaact gcagatggag aacaattgaa
    15181 aaaagacata ttagtaagag aagccgggga aaacctattt ttggcagtga agataactgg
    15241 attattcaaa aatatgcaca tccgtatcat gccgaagcaa gaaaaacctt acagaagact
    15301 agcaaaaatt tcagagtctc aaccagaatt ccaacaaagc aatatccaag gaacattagt
    15361 cgggttcttt acgcctgaat tattccaagg agttgctgca gcgggatttc acctgcattt
    15421 tatcgatgat acacatacat ttggcggaca tgtaatggat tttgaagtgg ctgaaggaaa
    15481 agttgagatt agccgcatct cgtcccttgt acagcatttt ccagttgatg atccgacttt
    15541 tgttcacgaa acgattgatt atgctgattt agcagcagaa atccaacaag cagaatagaa
    15601 aaagacagcg agatgcgttc aaggtgatta acctgacgta tctcgttttt ttgaggagga
    15661 aacctagcgt ttacaaaaaa actgagctat aatcttctcc atagatcaaa aaggagcggt
    15721 ttttatcatg tggacagaaa aacagaaaaa agatgcatac gaaattttat tattacagca
    15781 acaaggatta ttagaagcag aagataactg gttagcaaat ttagcaaatt catctgcatt
    15841 gttgaatgag actttgcctg agactgtctt tgcaggatat tatttattcg atggtaatga
    15901 attgatttta ggaccgttcc aagggcgagt ttcttgcaca cgtatcaaga tgggaaaagg
    15961 agtttgtgga gaatcagctg aaaaacaaga aacattgatc gttgatgatg tcaaaaccca
    16021 tgcaaattac atttcctgcg attcagccgc acaatcagaa atcgtagtgc caatggtaaa
    16081 agaaggtaat ttaatcggtg ttttagatat cgattgcgga gtaacgaaag ggtatgatga
    16141 aatcgatcaa gcatttctag aaaaatatgt agatctttta ttagcaaatt ttaatataaa
    16201 tgctttttca tttattaata actaaaatcg tctattgaaa caaaaaagaa gagaaatcaa
    16261 gaataaaaag agccggaaag acagtggtca tttctgtttt tcggtttttt tattttttta
    16321 taaaatcaca agtgttattg attttcacaa tcatatttta atggtattat cattttgaat
    16381 aatgaaaaaa cattaaggat ggatgaaata tgaataacga aactatcatt ttaggaaaaa
    16441 aatatatttg ccaacctatt ggcttgaaac aaaaagtggt tggagaagta atcagtaaat
    16501 tagaaaattg tgttgtgatc tgcgtagacg aatatcatga cgtcgatcgc aatgaaatat
    16561 tagaaaaatg cggaaaagta gtggcaaaat atactcacgt ttatgagttg gaaacaacag
    16621 aatatctata taactatagc aacaaatatg aacatacttt agtttcataa agatttaaat
    16681 aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc actttattta gttggcagtg
    16741 attgttcatg atttttgatt tattcaaaag gttcgcctag agaatctgtc tctgtaacga
    16801 ggcctggtgg agaaactcga taaaaaccta tactgccaga actgtcctat tttcggatag
    16861 acaaactagt agttttaaaa agatcatccg atccaatcat ttcaaaaaat gcaaaactga
    16921 tatcaggatc atttagctca ggcttgttat cgtaaagcat ttgtaaaggt tgcatttagt
    16981 taggagtaaa cgcgaaacta tcatcattat ttgctgaaga agatgaagaa gctaattcag
    17041 agtttgtttg aaataaagga gacgtgctga cagtttcttt accctgtttt ttgcagaggt
    17101 agaaactttt atatcggaag tttctaccgg agaatttttc tcatttgtag aaattgttga
    17161 atcgatacta ttcgaaattt gcgtatctga aggtatgttc tttgatgcag aagtcccaca
    17221 agcagtaagt aacaataaat ttgataaact gataaacaga attcttttca ttatgtacac
    17281 actccttata gtatcgttta gcatacacct atgctcagta ttaggcaaaa tcaatgcaaa
    17341 actatcaagt cttttatagt atctattagt cttagtaata catatatgga gagggtgata
    17401 tcccagttca aaccagatac attggttacc atctgtatta acgattatgc ctctaaacgc
    17461 tttttttatg aaaaaattta taagtgaaaa cgtaggtatg atattatcta gaggtaattt
    17521 ttttgggtat ttaaatagtt gctaggagga tagcttttgg atttttacag taattttatc
    17581 ttaataatag caatattatt acttctaaat atttggtttt ttgataagag tagaaatgca
    17641 gggataggtt ttagaaccaa acgaagtacg tcttctgaaa aaaagtgggt atattcacag
    17701 actatttttt acggaggggt tatttcaatc agtttacttt cttcaacact ttattctttt
    17761 aatgttatag acgtttccat gtcaaatttt atatctatta ttggaatcct tatttctgca
    17821 attattacac aactacttct tgtgtttgaa gagaagtcaa aaaataatta aagcaattat
    17881 gccttttagt acaggaatta ttacttctta caaatgtata gcgaatgtga agcctgctgt
    17941 tttaattgtt ctagaaaaac ttttgagtga acaattattt tatgttagga tgaattaatt
    18001 tctctagagt ttataattaa aaaacttatt tgaaggaaca tgcttaaacc ataattgtgt
    18061 ttttccttcc atcagcataa aagttcacct caaaaagagt gtacgaacaa aagttctttt
    18121 tgtaaacctt aaaatagatt ttcctgaata cgtattgggg atttctaaat gtttgaatgt
    18181 ttttttgaat gcaaaatcaa tgcaaaaaat tttgtatttc tctacttttc tctccttgac
    18241 caaaataaaa aaacgttgat ttaacaacgt ttttcataca tgtttatctg tctcaaccat
    18301 acatatatgg agacggcggg agtcgaaccc gcgtccaaac acatcgccac ttaaaaatct
    18361 acgttcatag tcaactcatt taaggattca ctttacagct tgccgagtga caggcattct
    18421 gtatcgctag tctgatgttc tctttttcaa cttacagacg gaaagttgaa acgtatccca
    18481 ctaaagttag gacccttact cgagcacatg ggcgatgccg agaggatcta cgctaactgt
    18541 ttttaggcag ctaaagcgta agagttgttt tcgtttttag cagttatatt taactgtaac
    18601 gtttttacgt agacgtaacc tacgaaacgc aattcaagct cgactgtgcc tgtcgaatcc
    18661 gtaacgtccc cgttagctaa caagggttac gtgtattttt catacaaaat tagtatagca
    18721 tatattggga taaattcaat aaaaaagcga atcatatccc attatttata taataactgc
    18781 ccctttctta agaaaaaata agcgattttc ttaagaaaag aggtagcttc aaaaaaagat
    18841 cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc gccttctgta tttttctttg
    18901 tgataaaggt agaaagttct tttaatccgt cataggcgtt gcccatagcg attttgttgt
    18961 cgcaagcttc caacaaggca aaatcattcg gaccatcgcc aaatccaaaa gtaggaactt
    19021 tttccagctg aagttctttt tgcagacgtt tgacacctgc cccttttgag attcctttct
    19081 tcactgtatc gatcgagtaa ggaccattcc gataaaaagt tagctcagga aaatgttcgt
    19141 gatagtattc gtcacctgat tgagataaga tcagcaacat atttactgtg ttttctttaa
    19201 agcctaaagg atcaatttcc ggaacatctg aatgaatgaa gtcgtaagca ttgatcattg
    19261 tttggctatg accggtacac cagattttct gatcgttata gtaagaaagt tcatgaccgt
    19321 tcaactgtac atgatcatac attttttgac attcttctga agaaaatgtt tgagaataga
    19381 tttcttcgcc gtctactcga atgaaagaac cattcatggt gaccgcagaa gtaatgcccg
    19441 catcttccat gatagagtgg atttctgctt ctgtacgtcc tgtagcgatg acggggagaa
    19501 cattgttttc ttttaatgcg ttcatagctt ttgccacttc gggtgtgatt tttgatttcg
    19561 catctagtaa tgttccatca agatcaaaga aagtgattgc acgataattt gacacaaata
    19621 aaactccttt tttattggaa taagtttatc ttaacaaaga atgaacaaaa gacaaacctt
    19681 tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt tagagtatac tataagaaga
    19741 gaatttagga gggaaagaat gatttttttc agtattggta tcttattctt aataattggc
    19801 agtctttttc ggattctgcc ctcaaaagga aatctgcctt tttatgggta tcattcgcct
    19861 cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa agacaagcgg aaactggttt
    19921 tttcttatgg gcttactgat ggcattgatc ggctattatt taaagacatc tgggcatacg
    19981 aattattttc tgattgaaat gcttctgcta gtatttccaa ttatgcctat ttttattatg
    20041 acagaaaaga aattacagaa atatgacctg gaaactggag ggaacgataa tgaatatttt
    20101 aatgattgaa gataatcaat ctgtttctga aatgatgcag atgtttttct taaatgaagg
    20161 ttgggaagca actttcaagt atgatggaaa agaagggctc gatgcctttt tagaaaaccc
    20221 acaacattgg gatatgatta cactagacct gaatttgccc acactggacg gtatgctagt
    20281 aggaagagag atccgcaaag tttcaagtac tgtgccgatt atcatgctga cagctagaga
    20341 ttctgaaagc gatcaggtga tcggcttaga gatgggggca gatgattatg tcacaaaacc
    20401 tttcagtcct ctgacgctta ttgctcgaat gaaagctttg catcgccgta gtgagttggc
    20461 agaagcaaga gaagaagcag cagtttcaga tgatcatttt gatatcgaga ctagtcattt
    20521 caagatgaat acgaaaacac gagaagcgta tctggacaac cggccaattg aaggactgac
    20581 gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa ccacgtcaag tattctctcg
    20641 ggagcagttg cttgaattag tctgggatta tcagtacttt ggtgatgaac ggacagtgga
    20701 cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc ggaccacaag tcatccaaac
    20761 tgtttgggga gtcggctaca aattcgatga ttctggtgtt gcctaatgcg ttatttgtat
    20821 cagcaactct tagctttttg cggaatggtc gcgttgatct tattgatcgt tggagtttct
    20881 tttacacaac tgacaaaaca aacgattgaa gaaaacaatt accagcaact gtttggatat
    20941 gctgaatcag ttgaaaagac aacgcaaacc tatgccgata cgctcccgca gtttagtcaa
    21001 gatcaggcgt ttcaaaatgc attgctgctg acagagcaag tactgacaga acaaaatgtg
    21061 aactttatat ttatcgataa aaacgaacgt gtcatttatc cgactaatac agctttactg
    21121 aattttacct taacagctga tcaatggaaa aatctgagga atggacgtca agaaaaattc
    21181 acatcgaata aaaatatttt aggacaagca caggcgacct cttacgcact tgtgccattt
    21241 aatttgaatc atgaattcta tggagcactt gtagttagtc aacctgcgcg aaatatcgat
    21301 aacagcgttc gtccaattac actgaacctg ttcaaaggat ttattttttc cagtatcgtt
    21361 gcagttattg ccagctatgc ttttgcagca ttccaagtta agcgtatcaa tcggttaagg
    21421 aatgcgacga aagaagtgac gaatggtaat tttgatgtac agctccccgt acatgacaaa
    21481 gatgaatttg atgagttagc agatgatttc aataaaatga cgaattcatt gaaagaatct
    21541 caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat ttatggcaga tgcttctcac
    21601 gagatgagaa cacctcttac gacaatcaac ggacttctgg aagggttaga atacaatgca
    21661 atccctgaaa atcagcgaga aaacgcgatt aaattgatga aaaatgaaac agagcgtctg
    21721 atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc gtacgaatca aatcagtatg
    21781 gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata ttgttgcaca gttagaagct
    21841 aaagcagaag cagcaggaga tacattgaca ttgaaagccg ttgatgatat tgatgtttac
    21901 gctgattatg accgatttgt ccaaatcatg gtcaatatta ttcaaaacgc gatacagttt
    21961 actgaaaacg gccaaatcac gattacttta gaaaaaggct atcttgaaac gatcatcacg
    22021 attgaagata cgggaattgg aatgtctgaa caacaaatga aaagtatctg ggatcgttat
    22081 tataaagtag atccttcaag aaaaaataca aaatatggtg aatcaggatt aggtttacca
    22141 atcgtgcagc aacttgtacg cttgcataaa ggaaaattag aagtagaaag cgaactagga
    22201 aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa tcaaaaaaga gtaaaaaaca
    22261 agcggtataa aagatcatga atcgatcttc tataccgctt gtttttacta taaaatgatt
    22321 gatttagccc gcgggctcta aacgatcaat ataaggactt tgagcaatag tagcatcatg
    22381 agtaaagagt aagatacctt gattttcttc atgagctaaa taagaaagca agtggagtag
    22441 ctcttggatc tccaatacac taagttcatc aaatgtatct tcaataacaa tattgtttcg
    22501 cttggaaagc aaggcatgaa gtaattgcag ctttactttt tcaaaagacg tcaactcagc
    22561 tgccgcacaa ttaagcaagg aaggagagat atcaagtttt tcttgatagg ccaaacgatc
    22621 ttgtttgatt tctttttccg gtacatccaa caggacatgg tctttaagat tcagataagg
    22681 aaacatcggc caattttttt ggatgatcgc aaaattatcc agttttgcaa caagaacctc
    22741 ttttttcaag tgctccaata ttttatgctg tttttcgaga ggacacaaaa taacggtcaa
    22801 gtgttcagca tgaatcaaat attcaggttt cattacgtcc aggcctccta ctttgcaaat
    22861 gatggataaa aaaagtgaaa gggaatagac taagaatgag acagcatcca ctgagcccaa
    22921 taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa aagatcattt tggaaatcga
    22981 tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc gcttaccgaa gtcaagatat
    23041 cacggatatc tggcagttca gtagtcttta agacaagaaa attgatcctt tgaagaagcg
    23101 cttcataaac tgactggaag cttacgatcc acaataaaac caagatacaa caaagaaaga
    23161 gaggaaagaa cagctgccag aaaataaaaa ggaataattt ggttttagaa agaccagtca
    23221 ggcgccaatt gatcaattcc tgcttgtttt gcctgactga atagtaaaag aaaaagataa
    23281 aaaggaaggc aaatagaaaa aataaactga atatgccttt tatataaaat cggctagcta
    23341 attctaaagc cggaagaagc tctgtagatg cagaagcgcc aataaatgaa agccgatctt
    23401 ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt caggcatgct atcaataaaa
    23461 aagcagaaaa gcttgaaaat aaacaataga gtattgtctg ctttttgtaa taaattaatc
    23521 gagcagtagc atagcggatt attttcatac ttttcacctc tataaaaagt atagaaaatg
    23581 aatatgaata aagtatgaaa ttcctttagt gaaatcacga attaaaacca gaaaatattt
    23641 tgtaaaaagc aagaggttgt gaagaagctg ccctattcta ttaccgtaga tgcaagcttt
    23701 tgaacactgt ttattcgaaa agaggagcag cgacaataat tttgtcgctg ctccttttaa
    23761 atttattttg tcattcggaa gaacttgtgg tcgttccgtg aagttcttgg tacgtttgcg
    23821 ttttataaag atcaacagtt gattgtccgt tgtgttcact aaagacacta gttgacttgt
    23881 caccaagaga tttttcagct tcttttaatt tctctaagcc attcttgtag tcataatcac
    23941 tggtatcaat tgggtctaat ccactattcg tatagaaccg aagaagatca ccattattga
    24001 tttggtcaga aacagctaac tgtttatcaa ctttttcttt cagtgaatca atttcattct
    24061 gtacttcctc agtaggttca gtaatagctt caccagtttc gttactatag atcgtaccac
    24121 cgtagtaagt atattttggt gtaacaaaat caccatcacg gaaagaaaca agctgttcat
    24181 gatctttcga gaatagatct tgtcctaact gaatataatt ttttgtatct acacctagta
    24241 agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta agtgtgattg attccgccct
    24301 gtgtttgtcc agggatatgg atcatataag ggacacgctg catttgtgca ttgtcgtagt
    24361 ttgtccaagt agaacttgtt tttccaacta attcagctag atttttattt cttgaattcg
    24421 agacaccgta atggtcaccg taaaggacga tgactgaatt atcatacaag ccgctttctt
    24481 tcaaataatt gaagaattct tgtacggcgg tatctaaata attggctgtc gcaaagtaac
    24541 cgttgatcgt ttcatcattt gtattggcta acgggaatcc tgcttcgtca ttggtgaatt
    24601 ttgaataagg ataatggtta gatacagcaa tgaattttga ataaaatggt tgttgcagac
    24661 gttccaagta ttgtacggat tgttggaaaa atggtttatc atgcaacccg tattggaacg
    24721 aattgtcatc attaacatca taataagaag aatcaaagaa ataatcatat cctaaattct
    24781 tgtatgtctc gtttcgattc cagaatgtac cagcgttccc atggaatacc gcactagtgt
    24841 aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa agtatttttt ccgcctaatt
    24901 gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt ttccatcagc gtttccgcat
    24961 cgcttgtttt tcctgctttt acttgatgga aaaagttgtc aaaactgaac gtgctgttgc
    25021 tgtggtaaag actattgatg aaaggtgtaa cttcatgctg gagaccattc tcatctgtca
    25081 atttataatc gatcaggaat tgctggaagc tttctaaatg gatataaatc acattttttc
    25141 ctttagcgag cccgaacaaa tcgtcatttg gtgctgcata atgttctttt acataatttt
    25201 ctacttcttt catatcattg ggacttgctt ctgcccgcac ttggtttgtc tggtaagttt
    25261 gaacaccgtc ataaaccatg aaagcattca agccaagata tttgaccaaa taatcacgag
    25321 agaatgttcg agtcagcaat tctggacgat cagcttccgc cataaaaaga tttcctgaga
    25381 aaatcataac tgctaatgta gagatcgcaa aagccattct tgctcggatc gggcgagggt
    25441 ccaacttgat tttttttgca atcagcagaa cagtcaagat aataaaatcg atccaataga
    25501 tcacatcata tgggcgaaac agtcgaatgg cactttctcc cagaccactc gctactttcc
    25561 cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa ttctcgataa taagtgacat
    25621 tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat catcgtgata taagcaactc
    25681 tagggcgtct gatataaaga gcaattccaa gtaataacaa tgtagttgca atagggttga
    25741 taaacaagat aaagtattga atgccacttt cgattccgag atggaaatca actaaatagg
    25801 caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga aaaaaatccg agcctcttat
    25861 tcaaaaaggc aggtgttttt atacatttca agataatctt cctttctgca cttcttaatc
    25921 acagaagtgt catgaaacca tgttacacag ctcacttatt ctcatttatt ttactagtat
    25981 gacaatatag tgtcaattga aaccagaagt ttatgctgtt tcattaaggt tttctttaag
    26041 cttcatgaaa aaatgagcag taagacaagt cctatgtttt ttgttatact tgggaagaga
    26101 aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa aagagcaagc aatcgattga
    26161 aaaaaggata tccactgatc caaaaagaag accttgctca tccccaaaaa gaaaccacgc
    26221 aatgggtgga attagttgat tcgcaaaacc aatttatcgc aaaaggatat ctaggaaagc
    26281 agaataaggg aatcggttgg gtattgagcc agcagaacat cccatttgat caatcttttt
    26341 ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt ctttttttca gatgattcta
    26401 ctaccgcttt tcgtatattc aatggagaag gagacggaat cggcggtctg attgttgatt
    26461 attatgcagg ttatgctgtt ttttcttggt ataacgaaac gttatatgca catcgtgaat
    26521 tattgattca agcttttcgt gaaatctacc cggaaatcgt aggaggctat gagaaaattc
    26581 gatttgaatc agtaaatctg cctgagagcc agttcattta cggaaaagag gcaccggaac
    26641 ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta tttaaatgaa ggattaatga
    26701 ctgggatctt tttagaccaa aaagaagtac ggggatcatt agcagaaggt ttcgcttctg
    26761 ggaaaacagt tttgaacatg tttagttata ccggcgcctt ttctgttgct gccgcaatgg
    26821 gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg cagtttaaaa aaaacacagg
    26881 aacagtttga agtgaatggt ttggatgtcg atcagcaaaa aatcattgtt atggacgtat
    26941 tcgagtattt caaatacgcc aaaagaaaac aatttttata tgacatgatt gtattggatc
    27001 caccaagttt tgccagaaat aagaaaaaag tctttcgcgt agcaaaagat tatggggaac
    27061 tggtcaagga tagtctggat attttagcag aagacgggat acttattgcg tctacaaatg
    27121 ctgcgaatgt ctcgatagaa caattccaaa caatgattga agaggaattc catgccaaaa
    27181 atgtttcttt tgaacaactt gctttatatc gtctgccaaa agatttccag acgacggata
    27241 cctttttaga aggcaattat ctgaaagtgt ttgtttatca aattaaaaat aaccattaga
    27301 aggagcgatg cagaatggtt tgggtaaagg gattgaatat aggaagcggt agaccaaaga
    27361 tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt acgtgaagca gttcttgcac
    27421 gtgaagcagg ggccgattta gtcgaatggc gagtcgatca ttatcgtgaa gttttttctt
    27481 ttgatgaagt agcagaaaca ttggttctta tccatgagat gcttgatgga ctgccgttat
    27541 tatttacgtt tcgtacctta gctgaaggcg gagaacaaga catatcgatc cgtcagtatc
    27601 gcgaccttta tgaacatgtg atacacacag gattagtaga tatggtcgat atcgagttgt
    27661 tcaaagtaga aagtctagga aaaagactga tagaggaaat caagtcttta aacattcctt
    27721 taattatcag tagtcatgat tttaaagaaa caccagcaga ccctgtcttg ctttaccgat
    27781 tgaatgttat ggaacatttt ggagccgata tcggaaaact ggcagtaact cccaataatg
    27841 aacgagatgt tttgcggttg atggagttga ctagacgggc aaatgctttt gtctccatgc
    27901 ctattattac gatgtccatg ggaaatcttg gtaaaatttc tcgtctggct ggcggaacga
    27961 cgggttccgt tatgacattt ggttccttaa gcagcgattc tacttctgct ccaggacaat
    28021 taccggtcaa agcattgaag caaattattc aattattaaa tgaaccgttg tagaacaagt
    28081 ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa cgaatgcaat ccgtatggtc
    28141 gaacaaaaaa agattcccta tacagagcac gaatatgagt gggacgaaag ccacctaagt
    28201 gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc gtatatttaa aacactggtt
    28261 gctgtgggca atgtgacggg gcctcttgtt gctgtgatac cgggagaagc cgaattgaat
    28321 ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag aaatgctcca tttaaaagat
    28381 ttggaagcaa cgaccggata catacgtggt ggctgctcgc ctataggtat gaaaaaacta
    28441 tttcctactt atttagatca aattgcagaa agctatgaac aaatcattgt ctcggctggc
    28501 agaagagggc tacagatgga gttagcacct caagatatct gtgcattaac gagcggacaa
    28561 tttgcagata taaaacaata aatcatacgt ataaaagaaa gaaggaggcc gggatgttgt
    28621 ttgtccagtc tccttctttc ttttttatac attatgctct ttgcccggca ttcattcctg
    28681 ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc gccagtataa ccattgacat
    28741 ccagtaattc tcccgcaaaa tacagaccat caatgatttt actctccaat gttttaggat
    28801 aaatttcctt tagagaaacc cctccgccag ttacaaatga tttctcaata ggaaaagttt
    28861 tgttgattga tagcttaaac tctttgcata gttgaacaaa atcttgaatt tgtttttgag
    28921 aggcttgttg tcctgtcaga tgatccattt caagccgctc aagaaagaaa actaacaatc
    28981 gctcaggcaa tataccatgc caagcattga ccaagttctt tttggttgct ttactttttt
    29041 ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg gaaacaatcc agagaaagtg
    29101 tcactggttc tcccgttttc tctaattctt tattgacaaa acttgaacaa cgcagtgcag
    29161 ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat cgtatgttca gtcaatacac
    29221 gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa agaaagccct tgaagtgttt
    29281 tctcctgaat atacggttca tccgatatca agggggactc tgtcgcataa agaggcgtca
    29341 ccgtgtgacc cactcgcttc acgatcttgt acccatctcc agtagaacca gtagatggat
    29401 aagtccgtcc accagttgtt aagataatgg caggagcatg gaactcttcg aattccgtgc
    29461 gtataccgta aattttatgt tctttgtgga tcaatttctc tactcgagta gagaatagaa
    29521 gcgtcacatc tagttctttc agacgattga gcaatgcttc gataatcgtt tttgatttat
    29581 ttgttacagg aaacatccgt ccatgatcct cttcttttaa atgtacgcct tgtgattcaa
    29641 agaagttcat gatatcgaaa ttattccatt gcgcaaacgt actatataaa aactttccat
    29701 ttccaggaat atgtgcaatc aaatcatcaa caggacgatt gttcgtaaca ttgcaccgac
    29761 caccacctgt catcagtagt ttttttcctg cttttttgtt tttttcgatc aagagtacgc
    29821 gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc gcttgttcca gcgccgacta
    29881 cgatcacatc atacagtttt tccattccct cacctcaagg aaagtgtagc atatctgaag
    29941 aaagaaaaca gaaaaaaaat gttttgagcc aaaagagaaa atgagctaaa atgagggaag
    30001 ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa tatgttcaag caattcaaga
    30061 aaagctacat caaaaggatc aagggcaaga agaattttta caagcaatcg atgaattcat
    30121 gcccacggtt atgtcttttt tggacactca tcccgaatat atcgaaaaga atatcttaga
    30181 acttttaacg gaaccagaaa gagttattca atttcgagtg ccttggcaag atgatgaagg
    30241 aaactggcga gtaaatagag ggtaccgtat ccaatacaac tcagctatcg gcccttataa
    30301 aggtggattg cgcttccatc ctagtgtcaa tctaagtatc ttgaaatttt tggcttttga
    30361 acagattttc aagaatagtt taaccggtct gccaatcggt ggaggaaaag gcggaagtga
    30421 cttcgatcct aaaggaaaat ctgataatga gatcatgcgt ttttgtcaaa gtttcatgct
    30481 ggaattgtca aaacatatcg gcccatcgat tgatgttcct gcgggagata tcggtgtagg
    30541 agctagagaa atcggctatt tgttcggcga atataaacga ttgaaaaaat atgatactgg
    30601 tgtgttgaca ggtaaacctt tggatttttg gggaagtaag atacgtacgg aagctactgg
    30661 ttatggatta gtgtattatg tcaaacatct attgaacgaa gaaaaagatt catttaatca
    30721 aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt tatgcaatcg aaaaagtgca
    30781 agagctaggc gggaaagttg ttacatgttc agattctagc gggtacatct atgatccaga
    30841 aggaatagat gtgaaactat tgaaagaagt caaagaaatc aatagacaac gtttgacgga
    30901 atacgcaaac caacgtcctt ctgctattta tcatgctgga gaatctgttt ggacattgaa
    30961 agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat gaaatagacg aaagcttggc
    31021 cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa ggcgctaata tgccatgtac
    31081 agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg tattgtccag gaaaagctgc
    31141 taatgctgga ggagtagcag tatctgcttt agaaatgagt cagaacgtcc agcgattgac
    31201 ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc atggaaacga tttaccatac
    31261 gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat ctgcttcttg gagcaaatgt
    31321 tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa ggtttagttt gaaaaatgaa
    31381 actttcttgg aaatattgtc agcattttgt ttatttgctt gaaaaataat aaaaaaaaac
    31441 gtaaagtatt acctgtagag taaacctata aggaggagaa caaatggcac acattcattt
    31501 tgactattca aaagtagcac cgtttgtcaa cgaacaagag ttagggtaca tgcaaagcca
    31561 agtaacagca gcccacaacg aattacggga aggtactggc gcaggaagcg acttccgcgg
    31621 ctggatcgat ttaccaacga actatgacaa agaagaattt gcacgtatca aagaagcagc
    31681 taaaaaaatc caatctgatt ctgaagtatt agttgttatc ggtatcggtg gttcttattt
    31741 aggcgctcgt gcagcgatcg acttcttgaa tcacacattc ttcaatttat tagatagcaa
    31801 cacaagaaaa gcaccacaaa tcttctttgc cggaaattca atcagctcaa catatattgc
    31861 tgacttgatc gaagtaatcg gtgatcgcga cttttcagta aacgttattt caaaatctgg
    31921 aacaactact gaaccagcga ttgcgttccg tgtattcaaa gagcttcttg ttaagaaata
    31981 tggacaagaa gaagcaaaca aacgtatcta cgctacaaca gataaagcaa aaggtgcagt
    32041 taaagtagaa gcagatgcag aaggttggga aactttcgtg attcctgatg acatcggcgg
    32101 acgtttctct gtattaactg ctgttggttt gttaccaatc gctgttagtg gtgcagatat
    32161 cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca tactcaagcg acaaactaga
    32221 agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt ctttaccgta aaggcaaagt
    32281 aactgagttg ttgatcaact atgaaccagg aatgcaatac ttctcagaat ggtggaaaca
    32341 attgtatggt gaatctgaag gaaaagacca aaaaggcatc tatccttcaa gcgcaaactt
    32401 ctcaactgat ttgcactctt taggacaatt catccaagaa ggacgtcgca atatttttga
    32461 aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca attcctgaac aagcagaaga
    32521 tttagacggt ctaggctact tgcaaggaaa agaagtggac ttcgtaaata caaaagcatt
    32581 tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct aacttgctag tcaaaattcc
    32641 agaaatggat gcttatacat tgggttatac aatgtacttc tttgaaattg ctgtaggtat
    32701 ttcaggttac ttgaatggaa tcaacccatt cgaccaacca ggtgtagaag cttacaagaa
    32761 aaacatgttc gcacttctag gcaaaccagg ctttgaagat cttgctaaag aattgaacga
    32821 acgcctgtaa gataagggtt tttcgctaga tttaaataat cagtgataga aacatgttcg
    32881 ctttaaaatg aaaactggta cccatttagg tactcaaaca tactgcatcg tttattcaga
    32941 ataaggggct gtgacaagac gtttgtcaca gtcccttgtt tgtatagaaa tcgattgatt
    33001 gccttgctct ttagtttgtt ttaggacttt aagaacagtg aggctgactt cgaaggttaa
    33061 ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc actcacttcg agtgcttttt
    33121 tgcgatatat tcttagctta agataggcct aatttttctt ttacagatga gggtacctta
    33181 tcgctggtaa ttactttttt ctctatgatc cgcttttcgc ttgctttcaa ctgcaagatc
    33241 gttccttcgg ggtaagcaga accattggca gagtcaccag aagtaatgac atcgaaatct
    33301 aactgtcttt tttcaccgtt ttcagcataa gcatctacgt tgtatttaaa acctcttcca
    33361 attacttccc cattatctgc tttgatatca gtttctgcag gcattggagc tttgactacc
    33421 gcataataat ctttacctac ataagtagac tgatagtagt cccaaccttt ccatcctata
    33481 aaagcaatca atatgataac gatcaaccct aaaatttttt tcatgaaaga tctccttttt
    33541 tatttttatt atacccgtgg tgctagttaa ttttaatatc ctaaagaata tctcaaagtt
    33601 ccttcaaaac gtcgttgtgc tcgacttaat agaacactgt aagccagtaa tatgctttca
    33661 aatctactct ctagcccttt aacagaacgt gagttgaaat tttgacaacc taatttttct
    33721 aaggaagaaa aaacagtctc aactgt
//
LOCUS       DANJLF010000025        32563 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000025 DANJLF010000000
VERSION     DANJLF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            188..2353
                     /gene="acm"
                     /locus_tag="PWT25_001308"
     CDS             188..2353
                     /gene="acm"
                     /locus_tag="PWT25_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL1021401.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2625..3110)
                     /locus_tag="PWT25_001309"
     CDS             complement(2625..3110)
                     /locus_tag="PWT25_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL1021402.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3200..3545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3590..4231
                     /locus_tag="PWT25_001310"
     CDS             3590..4231
                     /locus_tag="PWT25_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1021403.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4250..4885
                     /locus_tag="PWT25_001311"
     CDS             4250..4885
                     /locus_tag="PWT25_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1021404.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4903..5718
                     /locus_tag="PWT25_001312"
     CDS             4903..5718
                     /locus_tag="PWT25_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1021405.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6037..7473
                     /locus_tag="PWT25_001313"
     CDS             6037..7473
                     /locus_tag="PWT25_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1021406.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7662..10109
                     /locus_tag="PWT25_001314"
     CDS             7662..10109
                     /locus_tag="PWT25_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL1021407.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10339..11406
                     /gene="rlmN"
                     /locus_tag="PWT25_001315"
     CDS             10339..11406
                     /gene="rlmN"
                     /locus_tag="PWT25_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL1021408.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11599..12702)
                     /locus_tag="PWT25_001316"
     CDS             complement(11599..12702)
                     /locus_tag="PWT25_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1021409.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13255..13803
                     /gene="aac(6')"
                     /locus_tag="PWT25_001317"
     CDS             13255..13803
                     /gene="aac(6')"
                     /locus_tag="PWT25_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL1021410.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14349..16010
                     /locus_tag="PWT25_001318"
     CDS             14349..16010
                     /locus_tag="PWT25_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1021411.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16080..16427)
                     /locus_tag="PWT25_001319"
     CDS             complement(16080..16427)
                     /locus_tag="PWT25_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL1021412.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16511..17452
                     /locus_tag="PWT25_001320"
     CDS             16511..17452
                     /locus_tag="PWT25_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021413.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17453..18496
                     /locus_tag="PWT25_001321"
     CDS             17453..18496
                     /locus_tag="PWT25_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021414.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18502..19572
                     /locus_tag="PWT25_001322"
     CDS             18502..19572
                     /locus_tag="PWT25_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1021415.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19562..20515
                     /locus_tag="PWT25_001323"
     CDS             19562..20515
                     /locus_tag="PWT25_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1021416.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20527..21537
                     /locus_tag="PWT25_001324"
     CDS             20527..21537
                     /locus_tag="PWT25_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1021417.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21822..22643)
                     /locus_tag="PWT25_001325"
     CDS             complement(21822..22643)
                     /locus_tag="PWT25_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL1021418.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22740..23018)
                     /locus_tag="PWT25_001326"
     CDS             complement(22740..23018)
                     /locus_tag="PWT25_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021419.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23286..25295
                     /gene="metG"
                     /locus_tag="PWT25_001327"
     CDS             23286..25295
                     /gene="metG"
                     /locus_tag="PWT25_001327"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL1021420.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25774..26361)
                     /locus_tag="PWT25_001328"
     CDS             complement(25774..26361)
                     /locus_tag="PWT25_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021421.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26536..27858
                     /locus_tag="PWT25_001329"
     CDS             26536..27858
                     /locus_tag="PWT25_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1021422.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            28013..28789
                     /locus_tag="PWT25_001330"
     CDS             28013..28789
                     /locus_tag="PWT25_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL1021423.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28782..29351
                     /gene="rnmV"
                     /locus_tag="PWT25_001331"
     CDS             28782..29351
                     /gene="rnmV"
                     /locus_tag="PWT25_001331"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL1021424.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29344..30228
                     /gene="rsmA"
                     /locus_tag="PWT25_001332"
     CDS             29344..30228
                     /gene="rsmA"
                     /locus_tag="PWT25_001332"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL1021425.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30679..31308
                     /locus_tag="PWT25_001333"
     CDS             30679..31308
                     /locus_tag="PWT25_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021426.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31432..32541)
                     /gene="ugpC"
                     /locus_tag="PWT25_001334"
     CDS             complement(31432..32541)
                     /gene="ugpC"
                     /locus_tag="PWT25_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL1021427.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT        10861 a         5657 c         6743 g         9302 t
ORIGIN      
        1 atttacatta ggaaaaacct tgtaaaaaca gttttttggc atatttattt tttataaata
       61 tttgtaaaat aactatcaat tttttagtta aaagattgga aatgagtggg ggaggaattg
      121 aagaaaggat ttttgagaga tgatatagta ggttttattt ttagttaaaa taggggagat
      181 agaaaaaatg aaaaagtgta gaaatattat tttatcaatg ttatttatta ttactaatat
      241 aacatcactg attcctgttc atgtctatgc ggatgcaggc agagatatca gcagtaatgt
      301 cacttcgttg accgtggacc caacgaatat tacagatgga ggaaacataa aagtaaaatt
      361 ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat tatctttgga tcaattggcc
      421 aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa atcccattaa tgattgaaaa
      481 taaaaatgtt ggaactttaa cggtcagaaa agattctgcg caggttgtgt ttaatgaaaa
      541 tattaaaaac ctagactcag tagaaggttg ggggcaattt gagatacagg ccagaaacgt
      601 aaccgatacc ggtgaagaaa atacgggacc attcaccgtg acgagcggtg ataaaacagc
      661 tactgtaaat gtgacaaagc cagcttctgg ttcttcttct agcgtttttt attataaaac
      721 aggagacatg ctacctgaag atactaagca cattcgatgg tttttgaata ttaacaataa
      781 cggcacttat gttgaacaac cagttaaaat cagtgatgaa atccaaagtg gacaaagatt
      841 agatccaagt acgtttgaaa ttaatcaaat acacttaggt gaacagaagg tttatagagg
      901 agaagaggga atccaacaat ttttacaaga tttccccagc gctacattta atttttcagt
      961 aactgataat tacattgaaa taactatacc aaaaaacttt gtcaatctta ggaaaataat
     1021 ggtgagttat aagacgatca tagaaaaccc agaacaaata aattttgaga atcattcgga
     1081 agcttggttt aaagaattta ataaacctgc tgttgatgga gaaagtttca atcatacagt
     1141 taaaaatatt tcagcttctg gtggagtcaa cggtacagtt agaggcgaat tgaagatttt
     1201 caaatatatc aatgacacag agattggtat cccaaatgtg actttcgaat taagacgagc
     1261 agatgaacag cctattcaag gacagtctag tatcctgttg acttcgaatg aacagggaga
     1321 agctagtatc aaaggcctgc aagtaggaga ctatgttgta aaagaaaaag aagcacccaa
     1381 ttggattgat tttgatccac taagttcaaa tgaactcaaa ttctccataa acgaaaatga
     1441 tacagaggga gtcagtttac ctatttataa caagaaaaag gttacgaaca tcacagcaac
     1501 taaaatttgg aacggtggaa ccactccaag accgagtatt tattttaaat tattcagagc
     1561 aagtacaaac aattgggaac cagttcctga tgcagaaaca aaacgattag acaatggaat
     1621 aacttcagtg acttgggaag atatccaaca gtatgatgat agtggaaacg aatatacgtt
     1681 taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc ccttcaggtt atcaaaaaat
     1741 tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc atttcagtct caggacaaaa
     1801 aacatgggaa gacaatgaga accaagacgg caaacgaccg acgacgatca cagtaaatct
     1861 attagccgac ggtcaaccga tccaacacaa agaagtaagt gaaaaagatg attggcgcta
     1921 tcgtttcaca aatctgccaa aatacagaga cgggcaagaa atcatctata ctgtaacgga
     1981 agacaacgtg cccgaataca gtacgacgat tgacgggtat aacatcaaaa acagttatac
     2041 accggaaaaa acaaatatat ctataccagg tagccatata acaccgtgga aaaattttcc
     2101 agagaagaca gagagagaga aaattagaaa tctattctct caaaaattgc aaatatttgc
     2161 tcatacagga gaatccagcg aacaaagaag caattatatt agtaagagag ctgtacctaa
     2221 acagataaca aataaatcaa aaagcattct tcctaaaaca agtagtaaaa aatctagttt
     2281 tgctgtaatc attggtttat tgatagtaac tatctgtgct gggatattct tacaaaaata
     2341 taaaaaagtt tagttagaat ataatatttt ttgttaaata tgactgaaat caaaagaata
     2401 aaattccgat ttataatcga aaaaagaagc atttttatat atgcttcttt tttcgtagaa
     2461 tcaataaata tggatttggc taactatgga agaatactcg tattagtttt cagcgttcat
     2521 actagttgtt acattgtttg attggccaaa agaatttctg atatattttg tgctaaataa
     2581 agaaaaagac gcttgaattg ggaaataaga aaactactct atcattacaa attttcataa
     2641 actggcagat gttctaagta aacaagtatc ttttcaaagt cggaaaaatc cataatcaac
     2701 gtagtggtat tgatatttgg atgaaagcct acggtatctt gacgatcaat agcagcatcg
     2761 atcacgactt ggattttttt ctctttgtcg tgcatcaatg ctaatggagt cactgtacct
     2821 gctggaacgc ctaggaggtc aggaagctgt tcttcagaaa caaaagataa gcgcttttct
     2881 tttaattgtt cagctagttg ttttaaatcg gcgtgctttt catcaggcaa gatgacaaga
     2941 tagatttgtt ttccttttct tgatttcaat aatagatttt ttacttgcgg accaggtagg
     3001 gaaaaaggga catttttgac tgacgtaatc gcaggatgat cgactttttg aaaagagata
     3061 cccagttttt ttagtagttc ataagcttct tgttcggttg cacgactcat cgcgcatcac
     3121 cctccttttt ttattagtgt atcataaaaa agagttgaca aatgaaaaat aatcatgtaa
     3181 attacacgta aagttaaaac aaatgacatc gaaaagaaga gtagccgtta ggtgttttta
     3241 gagagtctct ggttggtgga aagaggcagc agttaaccgt gaaacacatc ttggagtcga
     3301 ttttctgaag cgtatagtag gaaaatccgg ggagcccgtt acagctgcgg tcgttgctag
     3361 atgaccagat aaggctggct tttccgtgag gagtcggtaa aaaaaggtgg aaccacgtga
     3421 ttaacgtcct ttgatcttcg gatcaaagga ctttttgttt tcatctgaac ggcaacgact
     3481 gtgtgaggtt ttttctgcaa ggaaaaaata aacagaggtg gaaccacgta taatacgtcc
     3541 tcttggcatt tgccaagagg atttttttat tttaaaggag gaaaagaaga tggagtattt
     3601 gattgaagtt atgccgcaat tattagtagg agcactcaca acattgaaat tatttttatt
     3661 taccttgatt ggctcattac cattagggat tgttttagca ttaggattgt ctttgcattt
     3721 taagccgcta cgctacttgc ttcaaacata tgtatgggtt atgcggggaa caccgctgtt
     3781 gttgcaactg atttttattt tttatgggtt gccgacgatt gggatcgttt ttgatcgttt
     3841 cgaagcagca ctcattgcct ttatcttaaa ctatgcagct tattttgcag agatctttcg
     3901 tggagggata caatcgattc ctcaaggtca atatgaagca gcgcaagttt tgcaattaag
     3961 tccctttcaa acggtgacga aaatcgtcat cccacaagtg acaaagattg ttttaccttc
     4021 tgtagggaat gaagttatca acttggtcaa agattcttcc ttgatctata tattaggttt
     4081 aggtgacgta atgcgtgctg ggaaaatcgc gatggaacgc gacgcgactt tactgccttt
     4141 gctaggcgtg gcaatcatct atcttacatt gactggtatt ctaacaatca taatgaaaca
     4201 agttgaagga aaattaaatt attaccgcta actgatagga ggaagaaata tgattgaatt
     4261 gaagaaagtg aacaaagcat ttggtgaaaa aaaaataatc aaagatttga atctaatcat
     4321 tccagatggg aaaacattag ctatcgtagg tccttccggt ggcggtaaaa ccacgctttt
     4381 acgtatcttg gctggcttag aaagttctga cagcggagaa ttttatttag acggtgtacc
     4441 gttcaaccca ttcgagctta aaatgaaaga acaagttgta ggagtcgtat tccaagactt
     4501 ccagctattt ccacatctga atatttttga aaacattacc ttagcaccaa aaatggtatt
     4561 gaaacagaca aaagaagaat acaccaaaaa agcagaagaa ttagcggctt cattagggat
     4621 tagtgattta ttgaatatct atccttatca gctttctggc ggacaaaaac agcgggtagc
     4681 gattgccaga gcattggcta tgaatccacg ggtattggct tatgatgaac caacaagtgc
     4741 tttagatcct gaattacgcc agcaagtcga gcgtttgatc ctatcattga aagaagatgg
     4801 ggtaacccaa attattgtca cccatgattt agtatttgca gaaaatgtcg gtgatcaatt
     4861 actaaaagtt tccccaattc attaagaaag gagaagatag agatgaaaaa aaccagtttt
     4921 ctattcgctt tgatcgcagg tcttcttctt tttgcaggct gttcgaacaa aaaaacttca
     4981 gcagataatt gggcgactat caacgaagag aaaaaaatcg ttataggttt agacgataca
     5041 tttgttccta tgggatttag agataaagac gggaagctca caggatttga tattgattta
     5101 gccaaagcag tctttgaaca atatgggatt acggtagact ttcagcccat tgactggtca
     5161 atgaaggaat ttgaactgaa caatggaacg atcgatttga tatggaacgg ctattccaaa
     5221 acgccggcaa gagaaaagaa agtgcaattt acaaaacctt atatggaaaa cgatcaagtg
     5281 ctgatcacac ctaaagaatc aggaatcaca aactttggac aaatgaataa taagagatta
     5341 ggtgcgcaaa atgggtcatc tggttatgat gtatttacga aacaaccaga agtattaaaa
     5401 gagatagtca aaaaccaaga tgctgttttg tatgatagtt ttaatgaagc attgatcgat
     5461 ctgaaaagca gacgggtcga tggtctgctg atggataaag tatacgcaga ctattatttg
     5521 aaacaaagaa acgaactaga agacttcaac ataacaaaag gttcttatga gagtgaggat
     5581 tttgcagttg gtgcacgcaa gtcagatagt acattagtga aaaaaatcaa tgaagcccta
     5641 gatcaactag agaaaacagg tgaattccaa aaaatatccg ataaatggtt tggcgaagat
     5701 gttacgccga gaaattaaca aataggggac tgtgacaaaa gacgtgtcac agtccctttt
     5761 tccgaataaa cagtgttctc ctgccaactc cttgtgactc ggagctgaat ggcaggctca
     5821 caacctcttg gtaaacggtg ttcaaaagcc agtttttcgg tattaagcca aatttatgca
     5881 aaaatgtaga aagccatttt cacaaaattt ttcttactac tcgaaactaa gcgacttttt
     5941 cacaacctcc tttacataat tttattttgt tgtggaaggg gtttcgtgtt attctaaaag
     6001 aggaacgatt gaaggttata ggaaaggaag agatgaatgg aacacaaaga acttacctca
     6061 tttccaaaag attttctgtg gggctctgct tctgcagcgt atcaagtaga aggagcttgg
     6121 caagaagatg ggaaaggaga atcagtctgg gatcgatttg tacggatacc tggaaagaca
     6181 tttaaaggaa cgaacggtga tttggcagta gaccactatc atcgttataa agaagatatt
     6241 gctttaatga aagaacaagg tctgaaagct taccgcttct ctgtagcttg gacacggatt
     6301 tttcctaatg gacgaggaga agtcaatcaa gctgggctcg ctttttatga gagattgatc
     6361 gacgaattga ttgaaaacga tattgaacct atgttgactt tatatcattg ggatcttcca
     6421 caagcattgc aagatgaata taatggttgg gaatcacgtc agatcattga agatttcact
     6481 cattatgcag aaacattatt tgaggcattc cgcgggaaag tccattactg gattagtttg
     6541 aatgaacaaa atatcttcac ttcattaggt tatttgttag cagcacatcc gccaggtgtg
     6601 acagacccga aacgaatgta tgaagttaac catattgcta atttagcaaa tgcatctgtc
     6661 atcaataaat ttcacgagtt agaaatgcct ggaaaaatcg gaccaagctt tgcgtatacg
     6721 ccaaattatc caattgacag taatcctgaa aatgttctgg ctgctgaaaa tgcagaagac
     6781 ctgatggcca attattggat ggatgtctat atgtggggaa aatatccgat tgctgccatg
     6841 aggttcttag aagaaaaagg atgggcacca acgattgaag ctggcgatgc agaattgcta
     6901 gaatctgcta aaccagattt tcttgggatc aattactatc aaacggcgac gaatgcattc
     6961 aatccattaa acggagttgg tgctggaaaa atgaatacga caggaaaaaa aggttcatca
     7021 gaagaaactg gagttcctgg aatgtataaa aaagttgaga atccatttgt cgaacgaaca
     7081 aattgggact gggagattga cccagaagga ttaaggatcg gactgcgtcg tatcacaagc
     7141 cgttaccgca tgccggtact gattacggaa aatggcttag gagagtatga caagttgaca
     7201 gaagataaac aaatccatga cgactatcga atcaattact tacaaagcca cgtcaaagcc
     7261 atcaaagaag caatttcgga tggagcagaa gtcctaggct attgcacctg gtcttataca
     7321 gatttgctta gctggctgaa cggttatcaa aagcgatatg gatttgtata tgttgatcaa
     7381 gacgaaacac aagaaggatc tttggaacga tataagaaag acagttatta ctggtaccaa
     7441 aaactgattg aagaaaatgg agagaacctt tagttcacat acttaaaaca agaaggtacg
     7501 gctggatatg gccgtgcctt ttcttgataa aatcatttag aaattaagaa aaactgtaaa
     7561 ataagctgaa aaatgaaaga aagcgtcagt tctttcaaac aaaactttag ttcccttact
     7621 tttttttgta taatgaaact tgatgaaatg agggataata aatggcacaa aagaaacggc
     7681 ctactaaaaa aaggaaaaag acgaaaaaaa cgaaaaagca aaaacagcag caggaacaaa
     7741 tgctgacgat tggtgttggg tttgccttga ttttatttgc tgtcttcggc tttttaaaat
     7801 taggtttttt aggaatattg attgcaaacg gctttcggat cattgcagga aatacttacc
     7861 aaatcctctg tcttttatta gccgtattag gattttggat tgtcattaag aatacagagt
     7921 tttcaattgg taaaaaccga cgctggtttg gcggcatact cttttatttc ggtatcttat
     7981 tattgttaca tgcccattta tttggcaaac tccatactgg ggaaccaaat atcatgggga
     8041 cgacatggga tttgttagcc agtgatataa aacaaagtca agtagacaat aacgttggtg
     8101 gtgggatgat tggagcaatc ctttatcatt ttacttactt cttgattgca cagcctggaa
     8161 gttatttagc agcagtgcta ttactggcag gaggagcatt cctgttcagt aatctggaag
     8221 gctatcagct gttaaatggt atccaatcaa ttggagaacg agtacaggaa ttattggaag
     8281 gtgatcctgc caagcaagct cgaaagcaag cggcaaagga agagagaatg aaacagcgcg
     8341 cagaagcgaa agaagcaagg cgcttagctg caaaagaagc tgcagaaaaa gaagctgcag
     8401 aatatgagaa gaaaaaagcg gtgagccagc aaagggaaaa tactgctgat gaatggcagg
     8461 aaccagaaaa tacagagccg gagcagctaa gttttgttcc aatcgacagt tttcaagaaa
     8521 atatccatcc tgcgaatcta gagaaaccag taccagatac accaaaacag accaatacag
     8581 cagaaggttt ttcagatgag ttgccagagg atgacggtac ctcattagaa tttgagattg
     8641 aagcagaaca ggaaaatcaa gactatgaat taccgacagt tgatttgctt gattctatac
     8701 caactgtgga tcaaagtgat gagtataaaa aaattgaaaa aaatatcggc gtattagaac
     8761 aaactttcca aagtttcggt gtcgatgcca aggtcgtcaa agccagctta ggaccatccg
     8821 tcacaaaatt cgaagtccag ccggctgtag gggtaaaagt aagtaaaatt gtgaacttaa
     8881 cagatgatat cgcgcttgct ttagcggcga aagatgtccg gatggaagca ccgattccag
     8941 gtaaatcact gattggtatt gaagtaccaa atggaaagat cagtatggtt tcctttagag
     9001 aaatcatcga agcacaacca aaccacccag ataaattact agaagtacct ttaggacgtg
     9061 atgtttctgg acgggtacaa acggctgatc tttcgaagat gcctcactta ctcgtggctg
     9121 gttcgactgg tagtgggaag tcagtagcaa ttaatgggat tatcacaagt atattaatgc
     9181 gtgctaagcc ccatgaagta aaactgatga tgatcgatcc gaaaatggtt gaattgaata
     9241 tgtacaacgg catacctcat ctgttgacgc cggtcgtgac gaaccctaga aaagcagcgc
     9301 aagccttaca aaaagtagta caagaaatgg aagaacgata tgaaaaattc gcggcaaccg
     9361 gagtaagaaa tatttctggt tacaatgaat ttgttcaaca gaaaaacttg gaaaacggaa
     9421 cgaaacatcc aaccttacca ttcatcgtag tgatcgtaga tgaattagct gatttgatga
     9481 tggtagccag caatgaagtt gaagatgcga ttattcgttt agcgcaaatg gctcgggcag
     9541 cggggattca tatgatccta gctactcaac gtccaagtgt ggatgttatc acaggaatca
     9601 tcaaagcaaa tgtaccttcc agaatggcat ttgcggtatc aagcggtaca gattctcgga
     9661 cgatcattga ttctaatggg gcagaaaagc tcttagggcg aggagatatg ctattcttgc
     9721 caatgggtga gaataaaccg atccgtgtgc aaggagcatt catttctgac cacgaagtag
     9781 aacgggtcgt tcaatttgtt acagatcagc aagaagctca ttatgaagag aaaatgatgc
     9841 cgacagatga agttgaaacg acaggtgctc cagaacagcc acaagatgag ctgtttgaag
     9901 aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat ttcccttctc caaagaagat
     9961 ttcggatcgg ttacaaccga gcagcacgtt tagtagatga actggaagct catggagttg
    10021 ttggcccgtc agaaggaagc aagcctcgga aagtattcat tgagcaggaa gaaacatcag
    10081 aaacgttgcc ggacgataat gacatgtaaa acttgtgtga tgaagggttg aaaaacgaat
    10141 aaccaataaa aagacaaggc atgatgatac caaaagaaaa aatggttttt ttccatcttt
    10201 tctaatacgg tatgatgtct tgtcttttta gtttttgtga gattaaagtt ctattcgatt
    10261 tatttgtgaa gtattgcatt aaggaaatga atcatgtata gtatgtagcg aaactttgag
    10321 aaaaagaggg aaaatacttt ggaaaaacag tccatttatg gcttgacgaa tgaagaatta
    10381 atcaattggt ttatagaaaa cggcgagaaa aaattccgag ctgcccaagt ttgggaatgg
    10441 ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga atctatcgaa acaattgatc
    10501 gaaaaattat cggagcattt tatcatcaat cctctaaaac agatggtcgt ccaagaagct
    10561 tcggatggta cagtaaaata tttgtttgaa ctccctgata aaaacatgat tgagactgta
    10621 ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga cacaagtagg atgtaatatc
    10681 ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc gtgatctgac tgctggagaa
    10741 attgttgcac agatcatgat ggtccaacat tattttgatg agcgcaatct tggcgaacgc
    10801 gtttcacatg tcgttgtaat ggggatcggt gagccatttg ataattacga caatgtaatg
    10861 gactttttgc atatcatcaa tgatgccaaa ggattggcaa ttggtgctcg gcacattaca
    10921 gtttccacta gtggattagc acacaaaatc aaggaattcg ctaataacgg cttgcaagtc
    10981 aatctggcta tttccttgca cgcgccgaat aatgaggtgc gaacatcgat catgcggatc
    11041 aaccgaagtt tcccaattga gaaattgatg gaagctgtgg atgagtattt agagaaaaca
    11101 aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag taaatgatag accagagcat
    11161 gcgcaacaat tagcagatct attaaaagat aagaaaaaat tagcatatgt caacttgatt
    11221 ccatataatc cagtcagtga acatgatcaa tattctcgca gtccaaaagc tgatgtattg
    11281 agattttatg atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa agaacatggt
    11341 acagatattg atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa aacagttgcc
    11401 aaataatcgt atcctactcg ttttttcaat taccataaaa ggagcagttt tcttaattaa
    11461 ggaactgttc aacttaaata agagaaaaga ttctatagcg aaagctataa agcttttttt
    11521 gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag cctgcttgat
    11581 gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt cttgtgtagc
    11641 atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga tttcataagg
    11701 cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta aatcggtcgt
    11761 gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca taaacacata
    11821 gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta caccataact
    11881 gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac cgtaatagaa
    11941 tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca atccggtagt
    12001 aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt cggcactatc
    12061 tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc cgatttggat
    12121 tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag attcgacttc
    12181 tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt cggcaataat
    12241 tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc cgtccattac
    12301 gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg aaccaatagt
    12361 gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag tatcgcaagc
    12421 acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat gctggatcaa
    12481 ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga caacctctat
    12541 ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag tcttccctcc
    12601 aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt catacgcatc
    12661 tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt ccttctttct
    12721 tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg tagatggagt
    12781 agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc ttgaaatcat
    12841 caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat aatgaatttt
    12901 tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct aaaaattcta
    12961 aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt ggtttttttt
    13021 aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc aattgatggc
    13081 tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttta ttttataagt
    13141 aagataaata atgaaagaat agagatactg attggtagct aaagattttt gagcgagaac
    13201 atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag acgtatgata
    13261 atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga tttactgaga
    13321 ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga aatgatgaat
    13381 ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt tattggtgca
    13441 atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga aagctcccga
    13501 cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt agcttccaga
    13561 ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac gttaagtcaa
    13621 acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct tcgtgaacat
    13681 ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc aaatgcaaat
    13741 ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc aaattctcaa
    13801 taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg aatttacgaa
    13861 aattacttct tgaatttttg tgaaattcca acttatttcc gaagaagcta cttttggaac
    13921 accgtgtacc agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg
    13981 aaaatggctt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    14041 caccgtttat tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat
    14101 tcttttttat ctttgtatgg caataataga caatataacg aaaattattt aatggctttt
    14161 ggaagaccat ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca
    14221 cgaaaaaatc attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt
    14281 cattaaactc ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg
    14341 tacgtaaaat gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg
    14401 ctggatgtta tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag
    14461 atggaggagg cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag
    14521 ttgcaacaga tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc
    14581 ttgatgaaga cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag
    14641 acggattaac ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg
    14701 ttacagcagc ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg
    14761 aatatgctta cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag
    14821 ataaatcaga actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga
    14881 aacaaacacc atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat
    14941 ccgtagtgga agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg
    15001 gaccattcac attagaagat tttgatggac caggaacaga tacagaatgg acttataaga
    15061 aaaatgatca ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag
    15121 taaaagaatc ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct
    15181 tgtctggtga gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag
    15241 cgcggacaag ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga
    15301 acctaagaaa agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag
    15361 gtgacggatc agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa
    15421 cgaacgaaga ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa
    15481 aagaatattg ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga
    15541 tggcttctga tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag
    15601 aaaacttgga tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc
    15661 gctcaacttc gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc
    15721 caagcagctt tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca
    15781 atgcagaata tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag
    15841 cccgttggga tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc
    15901 cggtgtacca aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc
    15961 atgcggcagg cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac
    16021 cgtgagcatg agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact
    16081 tatcctaaca aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg
    16141 atcagccaaa agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt
    16201 ttctttcggt tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc
    16261 gaagaaaaga cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga
    16321 tcattagata attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc
    16381 agaatcagta gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt
    16441 tatttttggt tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg
    16501 ggaaacatta gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat
    16561 cacgctttgg ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc
    16621 atatacgaat caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg
    16681 tttggataaa ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga
    16741 ttttggaatt tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat
    16801 cggaccatcc ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat
    16861 tttaggaacg atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt
    16921 agcgatatta ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt
    16981 tgcaatcaaa ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt
    17041 accaacaatt gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga
    17101 aatggtggaa gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg
    17161 ttgggaaatc gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt
    17221 aggaccgtta gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat
    17281 tcctggtatc ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat
    17341 ggctgtaacg atcctttact catttatgct gatttttgtt attttgatcg tggatctatt
    17401 gtacggtcta gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat
    17461 gataccaaag cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca
    17521 aaaaaatacc gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga
    17581 ttcatggcgc cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt
    17641 catcattttg atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa
    17701 cgtggcttat atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg
    17761 aaaagcaatg gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa
    17821 cgtaaatttc tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat
    17881 gggaatgaga atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg
    17941 tgtagcttac gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg
    18001 ttttttggaa atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt
    18061 ttttgaacca ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat
    18121 ggcacgtatc gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg
    18181 attgacatta ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc
    18241 tagtgtcatt attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt
    18301 tttaagcttt atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag
    18361 cgaagggtac aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt
    18421 atcggttatc atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc
    18481 taaaatgaaa gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct
    18541 tttgatacgt atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa
    18601 ggagaaacat tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc
    18661 atcatgcgct tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt
    18721 aacgatatcg ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg
    18781 atgattttcc aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc
    18841 gctgagtccc ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta
    18901 gaattgctta aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat
    18961 cagttctcag gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg
    19021 gaaatcctga ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt
    19081 ttggagctgc tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat
    19141 gaccttggtg tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc
    19201 gttgaagttg gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga
    19261 ctgcttggtt caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt
    19321 tctccaccgg atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc
    19381 gcgatgaaaa tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag
    19441 gcagcaactt ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa
    19501 cgtcgctggg ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct
    19561 gatgccaaat aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa
    19621 gaatgaagta catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg
    19681 tttagttgga gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa
    19741 tgaacctact gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa
    19801 aaaaggtttg acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc
    19861 gttgaatcct cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact
    19921 agctaaatca tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt
    19981 gaatccaagc cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat
    20041 tggtattgca cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc
    20101 tgcactagat gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga
    20161 acatcaattg acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga
    20221 ccgaatcggt gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta
    20281 taatcacggt gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc
    20341 agaccatgag agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt
    20401 acgtcaactt cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc
    20461 atattacgca aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg
    20521 acatagatgc taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa
    20581 ttgattcgta cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag
    20641 aaaagttggc aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa
    20701 gaatacccat tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg
    20761 gatcgggctt ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa
    20821 aaaagtatgt ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat
    20881 ggctacttaa agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt
    20941 gaaggatcga ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca
    21001 ctactttgtg atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc
    21061 tatcatccga agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt
    21121 caccagacag aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc
    21181 gctcaccata gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa
    21241 gtaatggatc tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct
    21301 gggaaattgg tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca
    21361 catgctgatt tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc
    21421 caatggtata ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc
    21481 gtaagaaaga gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa
    21541 atgaatgaaa ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt
    21601 ttgtttctag gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac
    21661 aatagcagaa aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt
    21721 ttacgaatac atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt
    21781 tttttcttga atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc
    21841 ttgagctgat agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg
    21901 aaatattgga gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct
    21961 aagaatttag caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt
    22021 agaacttgcg gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt
    22081 tgactatggt caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc
    22141 gagatagcca cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac
    22201 cagcgtactt ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag
    22261 tagctgactg tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt
    22321 tgctcaatat tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg
    22381 ctttctccag ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga
    22441 taaggatgcc cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag
    22501 cacatcagta cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt
    22561 tgataccagt taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct
    22621 tctttagaag caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc
    22681 ttattgtagc agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt
    22741 taaatatgaa aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc
    22801 aatactttat accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca
    22861 atgataaaac gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta
    22921 aaataaccat acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa
    22981 gcaatactaa gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa
    23041 gatagatagt ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag
    23101 atatgaaaca aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat
    23161 tgaacgtaga tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat
    23221 gcttttttta tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga
    23281 gccccgtggc agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca
    23341 aacttcatat cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac
    23401 ggttgatggg ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg
    23461 aaacaaaagc agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg
    23521 atgtcaagaa attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag
    23581 atgattacca taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata
    23641 tttacttagg tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa
    23701 cacaacttgc agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa
    23761 gtggacacga agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg
    23821 cagaccgtct attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta
    23881 aaaacgaaat gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa
    23941 caacttttac ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga
    24001 tcgatgcatt atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat
    24061 tcgaaaaata ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata
    24121 cgatctattg gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg
    24181 ggcatggctg gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt
    24241 atcctgaaat gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg
    24301 ctgttccgtt tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt
    24361 atgatctggc aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat
    24421 attgtgatgg actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt
    24481 ctacgacggc tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca
    24541 atacagcatt ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa
    24601 cagaaccatg ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga
    24661 tccatttagc tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa
    24721 cgccagagaa gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat
    24781 tacactttgg cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc
    24841 cacgtttaga tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc
    24901 aaacaaacga agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag
    24961 aaaaacaaat caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca
    25021 actgtcaaaa agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca
    25081 gccaagaccg tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga
    25141 tcgggaaaaa agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca
    25201 gtcaaggaat gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac
    25261 caaaatccat gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag
    25321 ttgtgtcata cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    25381 acttgaagcc aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa
    25441 gaagtacgag cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa
    25501 cagaaaaaaa gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct
    25561 tttttattgt ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta
    25621 tatctaacag tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt
    25681 tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc
    25741 gatgattgtt ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat
    25801 ttttgtcttc aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac
    25861 ataccagaaa atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg
    25921 taacaagatc aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat
    25981 gacttcacta agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt
    26041 catccgccag ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa
    26101 aataccgata ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat
    26161 cacaggacct ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg
    26221 tgataacgtt gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga
    26281 ctgctgttgt tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg
    26341 ataagaacgc ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata
    26401 cgaaaaataa aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa
    26461 aaaagaatgg taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga
    26521 agaaagagga tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta
    26581 gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact
    26641 gctagtactc agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg
    26701 atctgtgctg gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct
    26761 gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa
    26821 agcttgattt actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg
    26881 acgaacttat tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt
    26941 tctgtgacag ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg
    27001 ttgattgtta aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga
    27061 caaggaactg tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga
    27121 ttgagcagtg cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca
    27181 atggcagggg aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg
    27241 agttttgtaa cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt
    27301 gatcatcttg ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga
    27361 atcggtggaa agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt
    27421 tatacactaa ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc
    27481 agtaaacagc tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc
    27541 cagttagcag tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg
    27601 ttgatttttg tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga
    27661 aaaaaagcgc cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt
    27721 attgcgattc tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta
    27781 gcattgatcg gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa
    27841 aaacgctcag aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc
    27901 tagatcttcg tgctttttct atttcaataa aacaagttct tctattttga tttttgctac
    27961 ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt
    28021 tgattctcat acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca
    28081 gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat
    28141 tgaaaagtct ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca
    28201 tccgacagag gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac
    28261 tttaccaaag gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc
    28321 gaaagaagtc caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct
    28381 accaatcagt attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga
    28441 agatgttcga gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa
    28501 acgatttctt gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc
    28561 attagacgtc caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga
    28621 tgccccttat ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg
    28681 ttatacagta gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca
    28741 aacatggaca aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc
    28801 aagagattat agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg
    28861 cagatacgat agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac
    28921 atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa
    28981 taagaaaaat cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac
    29041 aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt
    29101 tagaagcatt gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca
    29161 ctagacaaga ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag
    29221 aaaaactagg tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt
    29281 tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac
    29341 aacatgagtg aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa
    29401 catggttttt catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta
    29461 aggaaaatcg tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca
    29521 ggaatcggtg cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag
    29581 atcgatgatc gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact
    29641 attgtacatc aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat
    29701 gaggagcttc cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg
    29761 atgcattttc ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa
    29821 gtagctgatc gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct
    29881 gttcaatatt atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct
    29941 cagccaaatg tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa
    30001 gtgacagacg aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa
    30061 acgctttgga ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta
    30121 gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta
    30181 caagaattcg ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg
    30241 tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata
    30301 gttcctcatg tttttcgttt gtttgggctt gttaccgcag atgcaagctt ttgaacaccg
    30361 tttaagaaag aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa
    30421 aacagcttct catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac
    30481 tattatgccg aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt
    30541 gatcaaaaaa ttcttatact tgatactcac actcattttg tatggattta gaaaagaata
    30601 tgtgtcttga cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca
    30661 tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta
    30721 ttgattggga tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct
    30781 tcctttactt tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt
    30841 gcagtggctg ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc
    30901 gcattgcgag cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag
    30961 catccgagta tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg
    31021 ttcaattttg tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt
    31081 tactttattg gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc
    31141 atcttgatgg ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt
    31201 atagcaggaa cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa
    31261 aatcggaaag agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc
    31321 aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc
    31381 ctaaaaagag ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag
    31441 tttgatgact tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag
    31501 gtcgactatt tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt
    31561 agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc
    31621 tgatttcact acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc
    31681 tggacgaata ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt
    31741 ttttccttca ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa
    31801 tgtcacatta aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac
    31861 gggtgtatca taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat
    31921 aacgattcgg tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt
    31981 tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc
    32041 atctaaattt gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg
    32101 tcccaatgcc acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta
    32161 ttctgtcaga cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc
    32221 atatttacgt aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa
    32281 tgcgtagttt tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac
    32341 ttttccgccg atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa
    32401 agtagttgac tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat
    32461 tgatagatta aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt
    32521 tttcagagcc atttctacca taatacgttc cacactttct ttt
//
LOCUS       DANJLF010000026        32178 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000026 DANJLF010000000
VERSION     DANJLF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(407..766)
                     /locus_tag="PWT25_001335"
     CDS             complement(407..766)
                     /locus_tag="PWT25_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL1021428.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="PWT25_001336"
     CDS             complement(796..1137)
                     /locus_tag="PWT25_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021429.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="PWT25_001337"
     CDS             complement(1134..1808)
                     /locus_tag="PWT25_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL1021430.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="PWT25_001338"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="PWT25_001338"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL1021431.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="PWT25_001339"
     CDS             complement(4322..5512)
                     /locus_tag="PWT25_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1021432.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="PWT25_001340"
     CDS             complement(5533..6060)
                     /locus_tag="PWT25_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL1021433.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="PWT25_001341"
     CDS             complement(6107..8896)
                     /locus_tag="PWT25_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL1021434.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="PWT25_001342"
     CDS             complement(9043..9243)
                     /locus_tag="PWT25_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1021435.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="PWT25_001343"
     CDS             complement(9696..10016)
                     /locus_tag="PWT25_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021436.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="PWT25_001344"
     CDS             complement(10519..11637)
                     /locus_tag="PWT25_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021437.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="PWT25_001345"
     CDS             12108..13415
                     /locus_tag="PWT25_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL1021438.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="PWT25_001346"
     CDS             13536..14735
                     /locus_tag="PWT25_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL1021439.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="PWT25_001347"
     CDS             complement(14762..16030)
                     /locus_tag="PWT25_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL1021440.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="PWT25_001348"
     CDS             complement(16200..16841)
                     /locus_tag="PWT25_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL1021441.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="PWT25_001349"
     CDS             complement(17250..17753)
                     /locus_tag="PWT25_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1021442.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="PWT25_001350"
     CDS             complement(17876..18640)
                     /locus_tag="PWT25_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL1021443.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="PWT25_001351"
     CDS             18748..19023
                     /locus_tag="PWT25_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL1021444.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="PWT25_001352"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="PWT25_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL1021445.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="PWT25_001353"
     CDS             complement(20579..20980)
                     /locus_tag="PWT25_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL1021446.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="PWT25_001354"
     CDS             complement(20994..21803)
                     /locus_tag="PWT25_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1021447.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="PWT25_001355"
     CDS             complement(21790..22680)
                     /locus_tag="PWT25_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1021448.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="PWT25_001356"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="PWT25_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="PWT25_001357"
     CDS             complement(23348..24289)
                     /locus_tag="PWT25_001357"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL1021449.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="PWT25_001358"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="PWT25_001358"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL1021450.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="PWT25_001359"
     CDS             complement(25274..26470)
                     /locus_tag="PWT25_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1021451.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="PWT25_001360"
     CDS             complement(26488..27216)
                     /locus_tag="PWT25_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1021452.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="PWT25_001361"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="PWT25_001361"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL1021453.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="PWT25_001362"
     CDS             complement(28628..29086)
                     /locus_tag="PWT25_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1021454.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="PWT25_001363"
     CDS             complement(29083..30546)
                     /locus_tag="PWT25_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1021455.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="PWT25_001364"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="PWT25_001364"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL1021456.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="PWT25_001365"
     CDS             31602..>32178
                     /locus_tag="PWT25_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1021457.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DANJLF010000027        31842 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000027 DANJLF010000000
VERSION     DANJLF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(151..2304)
                     /locus_tag="PWT25_001366"
     CDS             complement(151..2304)
                     /locus_tag="PWT25_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL1021458.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="PWT25_001367"
     CDS             complement(2598..3446)
                     /locus_tag="PWT25_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1021459.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="PWT25_001368"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="PWT25_001368"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL1021460.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="PWT25_001369"
     CDS             complement(5947..6126)
                     /locus_tag="PWT25_001369"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL1021461.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="PWT25_001370"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="PWT25_001370"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL1021462.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="PWT25_001371"
     CDS             8274..10553
                     /locus_tag="PWT25_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1021463.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="PWT25_001372"
     CDS             complement(10646..11293)
                     /locus_tag="PWT25_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021464.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="PWT25_001373"
     CDS             complement(11472..12191)
                     /locus_tag="PWT25_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1021465.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="PWT25_001374"
     CDS             complement(12322..13473)
                     /locus_tag="PWT25_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL1021466.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="PWT25_001375"
     CDS             complement(13690..14430)
                     /locus_tag="PWT25_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1021467.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="PWT25_001376"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="PWT25_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL1021468.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="PWT25_001377"
     CDS             complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="PWT25_001377"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL1021469.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16041)
                     /locus_tag="PWT25_001378"
     CDS             complement(15391..16041)
                     /locus_tag="PWT25_001378"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL1021470.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="PWT25_001379"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="PWT25_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL1021471.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="PWT25_001380"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="PWT25_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL1021472.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="PWT25_001381"
     CDS             complement(17484..18011)
                     /locus_tag="PWT25_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL1021473.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="PWT25_001382"
     CDS             complement(18191..18976)
                     /locus_tag="PWT25_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL1021474.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="PWT25_001383"
     CDS             complement(18969..19838)
                     /locus_tag="PWT25_001383"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL1021475.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="PWT25_001384"
     CDS             complement(19839..21218)
                     /locus_tag="PWT25_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL1021476.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="PWT25_001385"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="PWT25_001385"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL1021477.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="PWT25_001386"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="PWT25_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL1021478.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="PWT25_001387"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="PWT25_001387"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL1021479.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="PWT25_001388"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="PWT25_001388"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL1021480.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="PWT25_001389"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="PWT25_001389"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL1021481.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="PWT25_001390"
     CDS             complement(25176..25400)
                     /locus_tag="PWT25_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL1021482.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="PWT25_001391"
     CDS             complement(25453..26415)
                     /locus_tag="PWT25_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL1021483.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="PWT25_001392"
     CDS             complement(26425..26865)
                     /locus_tag="PWT25_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1021484.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="PWT25_001393"
     CDS             27171..28136
                     /locus_tag="PWT25_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL1021485.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="PWT25_001394"
     CDS             complement(28193..28423)
                     /locus_tag="PWT25_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL1021486.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="PWT25_001395"
     CDS             complement(28509..29396)
                     /locus_tag="PWT25_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL1021487.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="PWT25_001396"
     CDS             complement(29485..30708)
                     /locus_tag="PWT25_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL1021488.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31771)
                     /locus_tag="PWT25_001397"
     CDS             complement(30725..31771)
                     /locus_tag="PWT25_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL1021489.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8794 a         6970 c         5488 g        10590 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt tt
//
LOCUS       DANJLF010000028        30652 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000028 DANJLF010000000
VERSION     DANJLF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..290)
                     /locus_tag="PWT25_001398"
     CDS             complement(<1..290)
                     /locus_tag="PWT25_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL1021490.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRAD"
     gene            complement(292..489)
                     /locus_tag="PWT25_001399"
     CDS             complement(292..489)
                     /locus_tag="PWT25_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL1021491.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(883..2610)
                     /locus_tag="PWT25_001400"
     CDS             complement(883..2610)
                     /locus_tag="PWT25_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL1021492.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2603..3796)
                     /locus_tag="PWT25_001401"
     CDS             complement(2603..3796)
                     /locus_tag="PWT25_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1021493.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3983..4627)
                     /locus_tag="PWT25_001402"
     CDS             complement(3983..4627)
                     /locus_tag="PWT25_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1021494.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4721..4855)
                     /locus_tag="PWT25_001403"
     CDS             complement(4721..4855)
                     /locus_tag="PWT25_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL1021495.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4857..6989)
                     /gene="feoB"
                     /locus_tag="PWT25_001404"
     CDS             complement(4857..6989)
                     /gene="feoB"
                     /locus_tag="PWT25_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL1021496.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6986..7459)
                     /locus_tag="PWT25_001405"
     CDS             complement(6986..7459)
                     /locus_tag="PWT25_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL1021497.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7860..8084
                     /gene="nrdH"
                     /locus_tag="PWT25_001406"
     CDS             7860..8084
                     /gene="nrdH"
                     /locus_tag="PWT25_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL1021498.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8243..10402
                     /gene="nrdE"
                     /locus_tag="PWT25_001407"
     CDS             8243..10402
                     /gene="nrdE"
                     /locus_tag="PWT25_001407"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL1021499.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10452..11417
                     /gene="nrdF"
                     /locus_tag="PWT25_001408"
     CDS             10452..11417
                     /gene="nrdF"
                     /locus_tag="PWT25_001408"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL1021500.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11506..12645)
                     /locus_tag="PWT25_001409"
     CDS             complement(11506..12645)
                     /locus_tag="PWT25_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1021501.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12954..13667)
                     /locus_tag="PWT25_001410"
     CDS             complement(12954..13667)
                     /locus_tag="PWT25_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL1021502.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13921..14622)
                     /gene="nagB"
                     /locus_tag="PWT25_001411"
     CDS             complement(13921..14622)
                     /gene="nagB"
                     /locus_tag="PWT25_001411"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL1021503.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14856..16388
                     /locus_tag="PWT25_001412"
     CDS             14856..16388
                     /locus_tag="PWT25_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1021504.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16579..17781)
                     /locus_tag="PWT25_001413"
     CDS             complement(16579..17781)
                     /locus_tag="PWT25_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1021505.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17863..18552)
                     /locus_tag="PWT25_001414"
     CDS             complement(17863..18552)
                     /locus_tag="PWT25_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL1021506.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18658..19026)
                     /gene="rplL"
                     /locus_tag="PWT25_001415"
     CDS             complement(18658..19026)
                     /gene="rplL"
                     /locus_tag="PWT25_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL1021507.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19079..19582)
                     /locus_tag="PWT25_001416"
     CDS             complement(19079..19582)
                     /locus_tag="PWT25_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL1021508.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19786..19913)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19992..20681)
                     /gene="rplA"
                     /locus_tag="PWT25_001417"
     CDS             complement(19992..20681)
                     /gene="rplA"
                     /locus_tag="PWT25_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL1021509.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20785..21207)
                     /gene="rplK"
                     /locus_tag="PWT25_001418"
     CDS             complement(20785..21207)
                     /gene="rplK"
                     /locus_tag="PWT25_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL1021510.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21550..22218
                     /gene="sdaAB"
                     /locus_tag="PWT25_001419"
     CDS             21550..22218
                     /gene="sdaAB"
                     /locus_tag="PWT25_001419"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL1021511.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22231..23103
                     /gene="sdaAA"
                     /locus_tag="PWT25_001420"
     CDS             22231..23103
                     /gene="sdaAA"
                     /locus_tag="PWT25_001420"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL1021512.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23194..24213)
                     /locus_tag="PWT25_001421"
     CDS             complement(23194..24213)
                     /locus_tag="PWT25_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL1021513.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24386..25291)
                     /locus_tag="PWT25_001422"
     CDS             complement(24386..25291)
                     /locus_tag="PWT25_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1021514.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25350..25895)
                     /gene="nusG"
                     /locus_tag="PWT25_001423"
     CDS             complement(25350..25895)
                     /gene="nusG"
                     /locus_tag="PWT25_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL1021515.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25996..26166)
                     /gene="secE"
                     /locus_tag="PWT25_001424"
     CDS             complement(25996..26166)
                     /gene="secE"
                     /locus_tag="PWT25_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL1021516.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26190..26342)
                     /gene="rpmG"
                     /locus_tag="PWT25_001425"
     CDS             complement(26190..26342)
                     /gene="rpmG"
                     /locus_tag="PWT25_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL1021517.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26504..27148
                     /locus_tag="PWT25_001426"
     CDS             26504..27148
                     /locus_tag="PWT25_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1021518.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27204..27707)
                     /locus_tag="PWT25_001427"
     CDS             complement(27204..27707)
                     /locus_tag="PWT25_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL1021519.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27720..27766)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28304..29269
                     /locus_tag="PWT25_001428"
     CDS             28304..29269
                     /locus_tag="PWT25_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL1021520.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29368..30540)
                     /locus_tag="PWT25_001429"
     CDS             complement(29368..30540)
                     /locus_tag="PWT25_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021521.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30527..>30652)
                     /locus_tag="PWT25_001430"
     CDS             complement(30527..>30652)
                     /locus_tag="PWT25_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021522.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9354 a         6292 c         5417 g         9589 t
ORIGIN      
        1 taatctgctc gatataaacc atttattcca gcagttcttc caatttcact caaatttctt
       61 attaattcct cttgatattc agtgaatctt attttattag tataagcagt aaaatataga
      121 taattcacca atctatttat taaaggaccg ggatctaatt catatttttg tttttctaat
      181 tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc tttacttata
      241 gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat tttagcactt
      301 ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac aagtagttgc
      361 cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc cataatattt
      421 tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca aaatttttaa
      481 atgtttcata atctaatcac ccctaaatta atgtttttag actattattc taacattcat
      541 taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga ataagaattt
      601 aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga atgcacttcg
      661 tacataataa actttaatca cattagtctt tcatacctac gatcgatctc tttgactcgc
      721 acccataaat tcataatagt ttgtcggcgt ctttggacgt ttcgaagatg agctacgcat
      781 tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa tttggatatt
      841 tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt tccagctttc
      901 gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat cttttccatt
      961 gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat tgtctaatac
     1021 tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat ggatctgctc
     1081 ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt tggcaaacat
     1141 ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg cttgacctag
     1201 tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca agttgtcata
     1261 tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga ttcctatgat
     1321 tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt aggctggcac
     1381 tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt gatctttata
     1441 ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg catctgactc
     1501 gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg ttacatcata
     1561 gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg tatcccattc
     1621 aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc ctataccaat
     1681 cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt ttttactttt
     1741 ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct gtgtaaaacg
     1801 gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat tcccgacttg
     1861 tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga tcatagaggc
     1921 aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca tgatcgtact
     1981 tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc cgaatgaatc
     2041 agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa aacgaacggt
     2101 tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga taccgaaaaa
     2161 tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt caaggatcgg
     2221 tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg gtaccccaat
     2281 gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca aatcattcca
     2341 tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa gtcctggacg
     2401 tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag gagctggaat
     2461 cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt gtaaaaatga
     2521 aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc agtttgccac
     2581 tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt tctgctaatg
     2641 cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct acaactgcaa
     2701 gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg tctttcccaa
     2761 tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc ctagcttcta
     2821 acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca gccatcagca
     2881 tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca gattcgatat
     2941 tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa cgatcgactt
     3001 cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct tcacctgttc
     3061 cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc cgtaaagctt
     3121 cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc aggtccagta
     3181 attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct tcgtccatca
     3241 gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt tgcatcccac
     3301 cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt tctaatgctt
     3361 tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac ccgtattctg
     3421 tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc atattgattt
     3481 tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct tctccatcaa
     3541 tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc aatgtggatt
     3601 tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg tcaaaattca
     3661 cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc ggctggtgat
     3721 cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt aaatgtttta
     3781 cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc tttctgaaaa
     3841 aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa gaaatttgct
     3901 tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag ccaagttaat
     3961 atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa aaaaagtttg
     4021 tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct gccgtaaatt
     4081 tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg ataactgtgg
     4141 cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc attgcttttt
     4201 ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc ttcgtttgat
     4261 tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc ttctccacac
     4321 ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat tgcaatagga
     4381 aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt acttccatga
     4441 ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa gcattgtacg
     4501 tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg ttttcaacga
     4561 tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt tttttctttc
     4621 cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg ctctagcaaa
     4681 ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg atctgttcct
     4741 tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt aaacgagtat
     4801 agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc gccatcctat
     4861 tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg ctgacggaac
     4921 aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa cgctacctgt
     4981 ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata agctaaaccg
     5041 cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg atggatagcc
     5101 ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga ataagctgct
     5161 gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc ggatacttcc
     5221 gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac caatcctgta
     5281 atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac aactgccagg
     5341 attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc ataaaaatta
     5401 taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc tgctttttta
     5461 acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg aggaaaatga
     5521 tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg tgtttttttc
     5581 aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga aggagctacc
     5641 caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa ctttgcagaa
     5701 caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt ttcaatcgtg
     5761 cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg aatgaaggat
     5821 tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat cctcgccata
     5881 tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg cggtagaaat
     5941 cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg catccatccc
     6001 gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt accaaacaat
     6061 gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag ataatagatc
     6121 agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc aatctgatcg
     6181 attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag tgtacatagt
     6241 tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc gtccaatagt
     6301 ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc tatctcgtaa
     6361 tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg tttcttgccg
     6421 ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg tggatcatat
     6481 gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt ttcgattagt
     6541 ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc aagaccatag
     6601 gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat catattcaga
     6661 ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag atttcgttct
     6721 aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac caaataatct
     6781 cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg taggtcctga
     6841 atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt tacgcccggc
     6901 caattcccta catattgatt cgaaccagtc aagagattga acaagctagt tttcccgctg
     6961 tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc aagattagtt
     7021 cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg attggatctc
     7081 ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc atatccatta
     7141 accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc tctcctgctt
     7201 ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg acaaagattt
     7261 gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa atggcattgt
     7321 tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata ttttgcaaat
     7381 ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa ttctgctttg
     7441 cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt attaaaatca
     7501 ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc attctcacat
     7561 agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag cgaatcttta
     7621 gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca attttttctc
     7681 tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc aaaaacacaa
     7741 aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt gactttttta
     7801 ctaagagttt tatactatct attatagata ataagaatgt gttagaggag gcactcacca
     7861 tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc aaacggtttt
     7921 taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct gaagcagtag
     7981 actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat gcagaaacag
     8041 ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac taaaagaatc
     8101 aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc gcaaacttcc
     8161 aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat catcaaatac
     8221 gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca gttatttcaa
     8281 gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata aggataaaga
     8341 agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt ttctatctat
     8401 tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat tcattcaatt
     8461 gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac atttttcttt
     8521 caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa ccaacgacgg
     8581 ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt actttgcgaa
     8641 cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac gatatcagcc
     8701 agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg tgtcttgttt
     8761 cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact ctgctttgca
     8821 attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg aagccggcgc
     8881 tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga aattattcga
     8941 agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg tggtttattt
     9001 gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa atgcagatga
     9061 aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat tttatgaatt
     9121 gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa aagaatatgg
     9181 tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg caaatccaaa
     9241 cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa aattacaaca
     9301 agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga atccaatcga
     9361 tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa cgccttctgt
     9421 gattaacggc aaacaggaat atgaagtatt aggaacggat atctcatgta acttaggctc
     9481 gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc gtacgatgac
     9541 tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac cgatccaaaa
     9601 cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca cttatcttgc
     9661 aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata tttacttcat
     9721 gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa gaaaacaagt
     9781 tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa aatacgtaac
     9841 tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca tttttatccc
     9901 aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac tgtatcatca
     9961 aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata cgagtgcaag
    10021 tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg gtaaaattta
    10081 ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg cttatgatat
    10141 ggacatgcgt aaagtaatcg atgtttatgc aatggcacaa caacacgtcg atcaaggcat
    10201 gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat ggaagacaac
    10261 aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc acaaaggaat
    10321 caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg gaagcaatca
    10381 atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat tttaattaaa
    10441 ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa attgaagata
    10501 tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat acacgtatcc
    10561 cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa gatacagtag
    10621 gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt ggcatggatc
    10681 agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat attcaattca
    10741 tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat acgaaaaaag
    10801 aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa aaagctgagc
    10861 gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc agcgtctttt
    10921 tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta ggaaacaaca
    10981 aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct gtacatggaa
    11041 cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca gaacaagaaa
    11101 cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa gaacgttata
    11161 cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc ctacgctata
    11221 atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat actgccaatg
    11281 atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat gacttcttct
    11341 cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac gacgattatt
    11401 tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa taaaaagagt
    11461 ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt cacttgtttc
    11521 ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc tcaataaata
    11581 gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta tgcaaatcag
    11641 catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga tcgtcaaagg
    11701 atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat ctaattgttg
    11761 gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa acaaaccgac
    11821 aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat tcgtgattcc
    11881 gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg ccaaaaagct
    11941 cattacacca ataatcaaag catctaagat cgtactgctg atataggcag ataatgtttc
    12001 gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa agaagtgaga
    12061 caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa atggaacggt
    12121 aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac ttgcagataa
    12181 actgactaat atgatattga acaaattact aagagataaa tttgctgatg ctaattgttc
    12241 ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc atttgcttgt
    12301 ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca ttaactgagg
    12361 cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg tcaataaaaa
    12421 accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat cgaacatata
    12481 atagataaaa ccagcaacaa gcaatggtaa aaaaactgcc gaaagcattt tcaatattgg
    12541 actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga gtactagctc
    12601 cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc tcctccaagg
    12661 tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat tttcatttgc
    12721 ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt aaacaaaaac
    12781 acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata aacggtgttc
    12841 aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa ctatttttat
    12901 ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat catttattgc
    12961 gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc ctcgatcaca
    13021 tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta cactcggtag
    13081 tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt ctcttcaaaa
    13141 taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc taacttttta
    13201 gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat cccgataaaa
    13261 ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc agcaattgcc
    13321 cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat gatcgttccc
    13381 gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc atctgaacga
    13441 atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact agcgatctca
    13501 acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt acgcatacct
    13561 gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag catagcgatc
    13621 cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta aacaccctcc
    13681 agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt acctcttacg
    13741 aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac ggtgttcaat
    13801 cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt tttcctttgt
    13861 cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta ctattgaaaa
    13921 ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt gattttgcaa
    13981 tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga ttgcatcagc
    14041 tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg aaccgattcc
    14101 cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt tgtttgcatt
    14161 gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag caccaggttc
    14221 attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga ttggatgtgc
    14281 atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt ttccatctgg
    14341 tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga aatgacggta
    14401 gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag aagtcatttc
    14461 actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg tgcttcctgt
    14521 tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt caaatgcttt
    14581 ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg ctcctttata
    14641 ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta gtatagacca
    14701 aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt ttcataagat
    14761 ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact gtggacgctt
    14821 tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca ttaaacaaca
    14881 ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa ttaaatgctg
    14941 gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg attaaaatca
    15001 tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat attcacaaag
    15061 gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa tttctaaaaa
    15121 ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac caaacatata
    15181 gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg gaattagacc
    15241 aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg ttggggatcg
    15301 atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt tcaaaggtga
    15361 ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa ccaaccaatc
    15421 acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt gacggtgcgt
    15481 atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat atcgcagaca
    15541 tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg aaacaaaagg
    15601 aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa atcgaaaaga
    15661 ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca aaaagccgac
    15721 aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt aaagctgtct
    15781 ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact gatttagtca
    15841 tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt ggtgaaaata
    15901 ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca ctgatcggtg
    15961 agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc aattactttt
    16021 cacaagatct gtactgggaa aatccattag aaacaccact acaatattta agcagtcaat
    16081 ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg ttagttaatc
    16141 aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag gtgaaacttg
    16201 cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg aaccatatcg
    16261 atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga acagtaatca
    16321 tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt gagattgaac
    16381 caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag ttttgtcaca
    16441 actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag ctcaaaaaac
    16501 ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc agaacagctt
    16561 ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt taaaaacaac
    16621 tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta cacctaacca
    16681 gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc caataaagta
    16741 cataaacata aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg
    16801 tgtttgattg gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag
    16861 caaaatggcc aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac
    16921 aagtcccata tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg
    16981 tgcagcaact gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca
    17041 attgtaggcg ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc
    17101 aaacatgaaa aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg
    17161 cggcaaactt ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg
    17221 cggcattttc cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata
    17281 gatcatccgc caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat
    17341 ccccacgagt aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc
    17401 tagtaccgca ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa
    17461 agaagcgcat gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc
    17521 agataacgct gccattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac
    17581 taaaaacagt aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc
    17641 acttgtgcta acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat
    17701 attggcaaca acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg
    17761 ttttttttca ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg
    17821 aaaagattgg aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt
    17881 tccttgtttt ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac
    17941 acttgcaacc aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca
    18001 cgcatacaac acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag
    18061 aggcaatttc atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat
    18121 atagcctaac attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg
    18181 attcgtttcg gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat
    18241 gttaccaaga gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa
    18301 accagcaatg actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt
    18361 caatacgatc aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc
    18421 tgccttactc gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa
    18481 ccgatagccc actaccagta tcagaatgat tcccaacgag atcaatatca atcggattat
    18541 tttctttttc atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata
    18601 aaaacaaaaa agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta
    18661 tttaacagtt acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc
    18721 tttagatacg ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt
    18781 caagcctaaa ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc
    18841 tgcagttaat tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc
    18901 agctacagct acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag
    18961 gtcgttaagt tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa
    19021 tgccattata atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt
    19081 aagctgcatc tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga
    19141 ctggcgcttg tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg
    19201 ccaatgctgt gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt
    19261 ctaatgcttt tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt
    19321 cgttactgaa tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag
    19381 cttttttagc tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat
    19441 cacgaagttg tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga
    19501 ctactactga tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt
    19561 ttgcgataat tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt
    19621 tttgcactga aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca
    19681 cctattcact tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct
    19741 ttgctcaacc agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa
    19801 actctatgcc accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta
    19861 ggaaattaag gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact
    19921 agcttagcat gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt
    19981 ctaatattta attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg
    20041 atcgttacat ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca
    20101 tcatggattg ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt
    20161 ggtacatgga tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg
    20221 tttactgctt ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct
    20281 tttggtccta atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca
    20341 actacatcaa agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg
    20401 aaatctgcac cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca
    20461 gtttgtgttt taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct
    20521 tttttagggt ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg
    20581 tttgtttctt ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact
    20641 ttttttaatg catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg
    20701 tggttataac ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga
    20761 agctgctgtc ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc
    20821 ttcaaccatg cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc
    20881 agcgatttct cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc
    20941 tgaacctttt tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt
    21001 aacgaatgtg aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc
    21061 ttgatcagct gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg
    21121 acctagtgca ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt
    21181 aacgattttt tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta
    21241 attggagatg catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata
    21301 atgtgcaaaa caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt
    21361 tttctgctat ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa
    21421 tttttcgttc tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa
    21481 agcaaacaat aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag
    21541 gtggagaaaa tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg
    21601 attggtccaa gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt
    21661 atatttggcg aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc
    21721 tatcgggggc acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat
    21781 gatgaacgtt tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt
    21841 gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga
    21901 acacgaaagc tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa
    21961 ttgaatggat tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa
    22021 gatgttccag gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc
    22081 ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta
    22141 gatgatcctc aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc
    22201 gtaaattatt tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat
    22261 caagcaaaag cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt
    22321 ggccgttccc gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa
    22381 tcaatcaacg agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca
    22441 aaaaaattag atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa
    22501 gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct
    22561 acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg
    22621 cttgatttga cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta
    22681 gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt
    22741 tctgcaagtg caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa
    22801 gcagcacaag cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt
    22861 gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt
    22921 atctctgctg atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt
    22981 gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt
    23041 ggcttagctg ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg
    23101 tgataaagct ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa
    23161 gagaggacta attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac
    23221 ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg
    23281 ttcaaaaaca gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg
    23341 gccaatcgca actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg
    23401 gacaccagag tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa
    23461 tacttcaaag gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat
    23521 accatctgcc cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac
    23581 gataactgga agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg
    23641 ttttgcagct gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg
    23701 ttcgctatac gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc
    23761 ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc
    23821 ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg
    23881 gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata
    23941 agtgctgatg gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc
    24001 tttttcatgt gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc
    24061 aattccgaaa acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat
    24121 gattttcttt gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc
    24181 accgcgaata tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta
    24241 ggcaatgttt acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga
    24301 tccttctttc atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa
    24361 catttactct cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat
    24421 ctacttcttg gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt
    24481 cctctatttc atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg
    24541 gtactgttcc atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag
    24601 tggttatagg aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag
    24661 caggcccctt tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa
    24721 aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg
    24781 ttcctaaata gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat
    24841 ctatgccgta gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc
    24901 actcgttatt tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag
    24961 acaaatctcc tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta
    25021 ctgcttgctg atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc
    25081 tgtagccgtg gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg
    25141 ccgttttttc tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta
    25201 acgcaaacaa actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga
    25261 ttggttctgt catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa
    25321 tcatatgatc gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca
    25381 agttctgtac tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct
    25441 ccgtcaactt ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg
    25501 acttcgccaa cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag
    25561 atatgattga cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca
    25621 acgaatcctg tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc
    25681 atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt
    25741 ttgacttcag tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc
    25801 atactttgcg cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta
    25861 tgcaagacat accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa
    25921 ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac
    25981 gttgtggcta gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat
    26041 tacgtaaaac atcaatccga aaataataga agtttcgatg actactaacg tatctttacg
    26101 gagttgtttt ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa
    26161 tttcatgaga caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga
    26221 ttacaatatt tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact
    26281 gattttgtgt agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc
    26341 atttacttct gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact
    26401 ttttacttgt caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat
    26461 taatttgaaa ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact
    26521 tgtatataag aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt
    26581 aaaaatttta gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat
    26641 tttccgtgga aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat
    26701 gacgcttcct gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc
    26761 tttcttcaaa gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga
    26821 aaatcatttt tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg
    26881 gagcgtagaa caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt
    26941 ggctgctatt gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga
    27001 tatcgaaaaa gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc
    27061 gaaagtatgt gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat
    27121 tgaaaatttg caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg
    27181 ctttcttttt tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa
    27241 taatacatca atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca
    27301 taggttgtcc aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg
    27361 ctgatggtaa agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt
    27421 tgcaataaag ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc
    27481 atgataccaa aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc
    27541 aatttacggt taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc
    27601 cctgaggata caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca
    27661 tttacaacga ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt
    27721 ttctcctagt ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat
    27781 aattataaca gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag
    27841 cttaaacaat gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg
    27901 gtcataagaa ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga
    27961 aacttaaata aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct
    28021 agcaagcagc tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt
    28081 aatggatcat gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt
    28141 tcctagactt ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac
    28201 aagtttcttt cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag
    28261 gagtattaaa atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca
    28321 tatattgatc aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt
    28381 accggtatta aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca
    28441 cgatgctgag attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga
    28501 acagaaaaca atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca
    28561 tcaggtactt gatactttct atcagatgga agtagaattc ccagttgctt atattccagc
    28621 aggttctgga gatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact
    28681 tcagctgatt ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa
    28741 aataagtgaa aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat
    28801 cgtccatgcg acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc
    28861 attttcgtat ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct
    28921 ggtagatgca ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa
    28981 gcatcctttt tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt
    29041 cattgatttt gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt
    29101 acttatacag aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt
    29161 acgtatcgtt tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg
    29221 accttttgat atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt
    29281 ttgtcagaag ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt
    29341 tgtcacagtc ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata
    29401 gtttctttct ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa
    29461 gacacaacca ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact
    29521 gatcaagtta ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac
    29581 catttctggc aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat
    29641 aaaaatatct ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc
    29701 cgaaaagcta atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt
    29761 tatatcccac gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa
    29821 cagcagcgag taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat
    29881 gggcgcactc tggtttcgtt tactgatctc tttgcgatta aatgctagat tgatgacgct
    29941 gaaaccgcta aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt
    30001 caacagcttt ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga
    30061 gagtgccatt tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag
    30121 aatcagtgtt tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc
    30181 tgggcgactc tgtatcttga tcgtcggcat ctttcctact tcgacttgtt cggtaaagtc
    30241 tgcaggcaga tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt
    30301 ttgtgaggtt ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc
    30361 atcgactaca ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt
    30421 attttcaaca tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc
    30481 tatcagtaaa ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat
    30541 aacgcacccc tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca
    30601 accatagaag attggtataa aaactagcca aatcagtgta gaaataaagt tt
//
LOCUS       DANJLF010000029        29982 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000029 DANJLF010000000
VERSION     DANJLF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            138..476
                     /locus_tag="PWT25_001431"
     CDS             138..476
                     /locus_tag="PWT25_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021523.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(681..1256)
                     /locus_tag="PWT25_001432"
     CDS             complement(681..1256)
                     /locus_tag="PWT25_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL1021524.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1988..2569)
                     /locus_tag="PWT25_001433"
     CDS             complement(1988..2569)
                     /locus_tag="PWT25_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1021525.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2752..3378)
                     /locus_tag="PWT25_001434"
     CDS             complement(2752..3378)
                     /locus_tag="PWT25_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1021526.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3400..4731)
                     /locus_tag="PWT25_001435"
     CDS             complement(3400..4731)
                     /locus_tag="PWT25_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL1021527.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4886..5287)
                     /locus_tag="PWT25_001436"
     CDS             complement(4886..5287)
                     /locus_tag="PWT25_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL1021528.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5373..5579)
                     /locus_tag="PWT25_001437"
     CDS             complement(5373..5579)
                     /locus_tag="PWT25_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021529.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5674..7176)
                     /locus_tag="PWT25_001438"
     CDS             complement(5674..7176)
                     /locus_tag="PWT25_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL1021530.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7194..7649)
                     /gene="argR"
                     /locus_tag="PWT25_001439"
     CDS             complement(7194..7649)
                     /gene="argR"
                     /locus_tag="PWT25_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL1021531.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7747..8364)
                     /locus_tag="PWT25_001440"
     CDS             complement(7747..8364)
                     /locus_tag="PWT25_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL1021532.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8585..9958
                     /locus_tag="PWT25_001441"
     CDS             8585..9958
                     /locus_tag="PWT25_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1021533.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10033..11145
                     /locus_tag="PWT25_001442"
     CDS             10033..11145
                     /locus_tag="PWT25_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL1021534.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11217..12854)
                     /locus_tag="PWT25_001443"
     CDS             complement(11217..12854)
                     /locus_tag="PWT25_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL1021535.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13022..13630)
                     /locus_tag="PWT25_001444"
     CDS             complement(13022..13630)
                     /locus_tag="PWT25_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1021536.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14014..15165)
                     /gene="queG"
                     /locus_tag="PWT25_001445"
     CDS             complement(14014..15165)
                     /gene="queG"
                     /locus_tag="PWT25_001445"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL1021537.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15184..15936)
                     /locus_tag="PWT25_001446"
     CDS             complement(15184..15936)
                     /locus_tag="PWT25_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021538.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16077..17027)
                     /locus_tag="PWT25_001447"
     CDS             complement(16077..17027)
                     /locus_tag="PWT25_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL1021539.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17028..17867)
                     /locus_tag="PWT25_001448"
     CDS             complement(17028..17867)
                     /locus_tag="PWT25_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1021540.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17864..18610)
                     /locus_tag="PWT25_001449"
     CDS             complement(17864..18610)
                     /locus_tag="PWT25_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL1021541.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18848..20218
                     /locus_tag="PWT25_001450"
     CDS             18848..20218
                     /locus_tag="PWT25_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1021542.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20273..21169)
                     /locus_tag="PWT25_001451"
     CDS             complement(20273..21169)
                     /locus_tag="PWT25_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1021543.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21283..21453)
                     /locus_tag="PWT25_001452"
     CDS             complement(21283..21453)
                     /locus_tag="PWT25_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021544.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21450..22127)
                     /locus_tag="PWT25_001453"
     CDS             complement(21450..22127)
                     /locus_tag="PWT25_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1021545.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22218..23123)
                     /locus_tag="PWT25_001454"
     CDS             complement(22218..23123)
                     /locus_tag="PWT25_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL1021546.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIEVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23298..24752
                     /locus_tag="PWT25_001455"
     CDS             23298..24752
                     /locus_tag="PWT25_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1021547.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24768..25799
                     /locus_tag="PWT25_001456"
     CDS             24768..25799
                     /locus_tag="PWT25_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL1021548.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25806..26993
                     /locus_tag="PWT25_001457"
     CDS             25806..26993
                     /locus_tag="PWT25_001457"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL1021549.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27149..28531
                     /locus_tag="PWT25_001458"
     CDS             27149..28531
                     /locus_tag="PWT25_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL1021550.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28503..28889
                     /locus_tag="PWT25_001459"
     CDS             28503..28889
                     /locus_tag="PWT25_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021551.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29089..29757)
                     /locus_tag="PWT25_001460"
     CDS             complement(29089..29757)
                     /locus_tag="PWT25_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL1021552.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8403 a         6141 c         5483 g         9955 t
ORIGIN      
        1 ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc aaatactata
       61 gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa atttcaaaga
      121 caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc ttttgctctg
      181 acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca actgttcgga
      241 tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact agacaagact
      301 ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt ttacatgatc
      361 caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga gaatatacaa
      421 ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag tcataatgat
      481 aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca aatagaagct
      541 atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt atgagcattt
      601 cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt tgccgctctt
      661 tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat ctgctgagta
      721 atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc ttcagtcaag
      781 acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt agtcattatg
      841 attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat atacactatt
      901 ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcata aaagccactc
      961 attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact
     1021 gtctctgcac gggcattaat catcaattgt ccttttttaa atccggtaaa tccccacttc
     1081 atgatttccg gagtgaggat gttttcttcc tcttttctta acgtcacaac aagatttgac
     1141 ggaaaaactt ctccagctgc taattctgct gcctgcttcg gttgtttcgt ctgggcttct
     1201 cgataataat tctgaaggag ctctgactct agatcaaaaa aataacgacc acacatccta
     1261 atccctccca atcttttttt ctatcataca ctgcaacaag aagttcgcac caacaaaacg
     1321 ctttcaattt ttgtagaact aaaaaagttg tgacaagtga caaactctcc agtgaatggt
     1381 attctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgctt
     1441 tttgaaattg ttccttgtga ctcggagtga aacggcaggc ttacgacctc tgggtaaacg
     1501 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt agaaagccat
     1561 tttcacaaaa tttttcttaa tactcgaaac taagcgactt tttcacaacc tcttccttaa
     1621 acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagcag
     1681 ctatttttat ttggctgttc ttattacttg atgcagggca gctttttcac aacctctcca
     1741 ctaaacggtg ttcaatcagc taaccttcgg tattagctca aaaaacggca aaatatgagg
     1801 aactattttg ccgtttgttt ctctaatact caggtcaaag cgctgatttc acaacctcta
     1861 atacacggtg ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaatttg
     1921 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct
     1981 ctaattatta aagaaccttt tgcgaagtta acaatctttc tttgatcaat tgaaaattct
     2041 gcctaagtag tgatagatcc tgtgtgactt tcaacaataa gatacttccc tggatttcag
     2101 caatcaacgc tttggcttga cggtctgctt cttcatcgga cagtttttct tcagaaagtg
     2161 ccaggatatt ttgtttcgta tacttgaccc attgattgac taattctagt agcaacagac
     2221 ggtattcttc atccactgta ctcaactcaa taatcaaatt acctacagga cagccatgat
     2281 aagtgatctg tgtctcatga tcagcgaaaa tccagtccaa catttcttct aatgcttctt
     2341 ctggctgttt tccagcattt aagatgtctt cgaacaattc tgtctgccat acgtccatat
     2401 gcgcatggat caccgctaga caaacttctt tttttgaaga aaagtaataa taaaattgac
     2461 cttttcctgt atcagaagct gctagaatat ctcggattga cgtttcatga tagccttttt
     2521 tataaataag atctttggtc gcttccaaga tcttttcctt tgttttcaat gatattctcc
     2581 tttttcattt ctcatagtcc tatattatac agtaaattca tcattttttc ttcttaatcg
     2641 tttaaaaaag actgtgccaa attatctgac acagccctca ctataaaact gttctcatat
     2701 agctagatgt tcattgattc aagaactgcg aggacaaaag caattcctat cttatgatag
     2761 ttgctctaat acttcctctg ttgtcatcac ttgatctgca ataaagccaa agttcgtcaa
     2821 cgctgcatcc attgcttctt ggccaggagc accaattgca tctgtcgcta ccacaacatt
     2881 gaatccactc tctaccaatt cacgcatatg ggaatcgcta cacaagtttc cgttcattcc
     2941 accaagaatt actgtgtcga ttccattttt tctcaattgg agcgccaaat cattgctttc
     3001 tggtccaaag attttatgag gagagctgat gatcgtattt tcatccaaat aaggtttcaa
     3061 ttcttcgata aaatcggctc ctgaaccttc ttccactgat tcatattgac tttttctagc
     3121 aaacatctgc tgctcaagca tcatatgttc tcctgcaccg ccaaattgcc atttatggtc
     3181 atgtgggtaa aaataatgag gagaaataaa gagtttgtat cctttttctt ttgcttgttt
     3241 taccatttca atcatatttt cgatcgtatt gtatttttct aagatatctt ttgtcaaacc
     3301 aaagccaccg cctgctggtt tcaagaactc gacttgcggg tcagtgagaa ctactgctgt
     3361 atttttatta ttgaacatgg tggattctcc attcgttatt tattttaata catcatttag
     3421 agcttcactc attgtcggat gagaatagat ttgatctctc aataacgtat aaggaagttt
     3481 tccattcata gctagcgaaa tcaagttgat gacttcatga gaatcttccg catagatact
     3541 tgcccctaga atcaagtcag tttcagggtc gactaaaact ttgaatacac ctttaggatc
     3601 ttctaatact tgtgctttag ggacaccgct tgtcatatac ttgaataatt tgtaattgac
     3661 gccttgtgct ttggcttgtt tttctgttaa acctacattg gataaagtcg gagtgataaa
     3721 tacactgtaa ggcacagctt tccggttatt cgttgttcgt ttattctcgc ccttcaactg
     3781 atccataatg attcgataat catctagaga gatataagtg aactgcaatc cgcctttgac
     3841 gtctccaatc gcccaaatgt tgtctgcatc tgtacgaagg tattcatcca cagcgattgc
     3901 tccgcgatct gttagtttaa catctgtatt ttctaatcca agtgatgctg tacttggttt
     3961 tcttccagtt gcagccaaaa cttttgacgc ttggattgtt acttcttggc catcttctgt
     4021 aaaggtgacg gcagctgttg attcttggtc tgctacttga tctacagata cacctagatg
     4081 gaattcgatt cccgcatttg tcatgtcttc taaaatcatt tgcgcgatat cttcatcttc
     4141 acgtggaaga aattcagaat gggcatcaag aacgacaact ttagatccgt attcattgaa
     4201 cattgatgca aattccaagc caatatatcc agccccgatg atcactaatt tttccggtaa
     4261 ttgttcctga tccatcgctt gcgtagaatc aatcaagtag cgacttgtat tcaatccttt
     4321 gattggtaaa atgattggtt cagaaccagt attgatgaaa atccgttcgc cttcaacaat
     4381 cactttttca ccattattcg tcacttcgat ttgatggtta gaaagaaaac gtgctgtacc
     4441 gtccatcact gtcccagttg cctcatcgct gatcatatga taatttttgg cgcgaagcat
     4501 gcccgtcaat ttttctttcc gagcaacagc ctctgtaaag tcgattcctc tttctccatt
     4561 gaaaatcaat gatttagaag gaatacagcc gatgttgata catgttccgc catacatacg
     4621 tgttgacttt tcaacgacta aaaccgattc accttttgaa gctaacgcct tcgctaaagt
     4681 cttaccgccc tttccaaaac cgatcacaat attttgatat ttttccatca tttttccctc
     4741 gcttcttttt tacctaccga ttggtacaaa aatatgataa ccgataattg tctattctcc
     4801 aaatcatctg ctttatttgt caaaaaaaac cagacaaaaa gcggtccttt cacgttttta
     4861 gtctggttcg atttccttta ttcttctaaa caatcgttac ttctacatcg atatttcctc
     4921 tagttgcttt ggagtaaggg cagaattcat gcgcatcttc tgcaattttt tgggcttctt
     4981 cttcgctgat gccatcgata tgcacttcta atactacacc gatctgatag ccgccatcat
     5041 ctaaattata aagagagaca cgtgctttta ctgtggactc tgctgtcatt cctgcttctt
     5101 tgatcatatg ttgtaccgcc ccattgaaac aagaagcata acctgctgca aataattgtt
     5161 ctggatttgt tccttctgca tggattcctg gaggagtgat tttcatatgc atcgagccat
     5221 ttggtgcctc gacttctcca ttgcgcccgc catggttgat cattgctgtt tcgtatgctt
     5281 ttttcattgt actgctcctt tcgattgacc atttgctact tggctataat tatagtgtat
     5341 gacaagtaag gcgctttcta caaaaattct gcttattcga ataccagaat gccgtcttct
     5401 actatttttc caaaaaacaa aaaaatacta ctcgtgaaca acagcgtcat atttgttcca
     5461 aaataaatag caatcttttc tacataatct tttatatgat tgattttaag atacatatat
     5521 accagaaagt tgaccccata aaccaatact gttaaaaaaa taaccatatt cgttaccatt
     5581 ttttcctcct tttgctggtc tccctaccag cactcgattt aaccatacca ctttttttgc
     5641 cgtccaagca actagccttt caaaaagact ggtttattct gctgttaatg attcaagatc
     5701 agaaaaatgt ccgaataatt ttgctaattt tactaacgcc aacttgttct ttccgaattc
     5761 atcattttct atctgtaata ctagtaacgg ctcatgtaag cttaaacgga tcaaaaacca
     5821 gccgtttccg tatggctctt ctagatcata tcgaatacct tcttcatttt ccgggtcgaa
     5881 gttcatccct ttttcttctt ccaacagtcg cttcatttct tctaatatct tgtttccttg
     5941 ttcgcgtggt tcatcgctga acaattttaa tcggatttct tgtacttcta cgggctgttt
     6001 caactctttg atttgttcat ctaatgtgat accttttctt tttaattctg gcaaaagcat
     6061 caggatcttt gcgatgacat atgcaccatc atccagaaaa tagttttcct tgaatgcagc
     6121 gtgtccgctt gtttcgatgg ctagctgtgt atctttccca agacggttaa gttctaatgc
     6181 tttattgatt acatttcgat agccggaaat ataacgaact tggatgcctc ctttttcttc
     6241 aatgaatctt tgtagatgag aagaagtagg ggaattcgtc acgattcttg ttcctggatg
     6301 ctcttttaat acgattgtac ttaatactgc gatcaaatta tttcgattga tcactgttcc
     6361 agaagcactg acaactgcag aacgatccac atctgtatcg aaaatgatcc caagatctgc
     6421 ttcatttgct aaaacagcct gcttgatact ttccatcgct tctttattat caggatttgg
     6481 gatatgatta ggaaatcggc catctggttc caaaaattgg gaacctgttg aatccgcacc
     6541 taagctattc aatacttttt cagcaaagaa tccaccagca ccatttccgg catcaacaat
     6601 gattttccaa ccatttagtg gtttgctttc tagtgttcca atcccctttt taatttttgt
     6661 gactaaatct tgcgcataag gtgtcaaaag gtctgctttt tctacgcttc cagtttgttc
     6721 gctttttatt ttttgctgct gtgataggat aaaagcaata tcttcttttt ctgctcctcc
     6781 ttctgaggta aagaatttta ttccattata ataataggga agatggctag cagtcagcat
     6841 aaccgcagca tcgcacgaga actgttcaaa ttgagtggac ataaacatag caggcgtagt
     6901 agctaattcg aaatcgatga catgtatccc ttgttctttc agactttgga tcaacgcctc
     6961 tttcagtgct ggtccgctta tcctactgtc atgaccaatc ccaatcgtcc attttttatt
     7021 tggatcggtt gatttcttct ttttctttat ccagtttgct agtccccagc cgatcttttt
     7081 taccatgtct gtcgtcaagt ttacttcata tccttcaact ggtaatgcga ttcctcgaat
     7141 atccgatcca ttttgcagcg ctttcatatc tttcatataa aagtcctcct catttattcc
     7201 ctcatttttt ccagcttttc tgcaaatcgc tgtgcttctt cttctgagcg gcaaataata
     7261 atgaatgtat ctacaccggc catcgttccg acaacttcag ggtaagccac ttcatctagc
     7321 caattcgtta ccacatcggc accatttcgt tcagtcaata cgataaccat gaactgaaca
     7381 gactggatac gcaaaacctc gcgtctaatt gctgtacgca aacgttcttc atttaaagaa
     7441 acagatggct ggttaaataa tgtatagcgt actattttgt tttcattttg actttttact
     7501 aacttcattt ctcgaatgtc tcgagaaatc gttgcttgag tcacatcggc gcctgcttca
     7561 tgcagccgcg caagcagctc ttcctgtgtc ccaataggtt gttctgtaat gatctggcgg
     7621 atcagtgctt gtcgctgttt ctttttcata ttagacacct ctcaatgttg attatagcaa
     7681 aagataagta atatttcttc atattttggt attttttact tttatttttt gttttcattc
     7741 aggttgctaa cctttttcta ttttaacacg caaaatcgtc ttcatttttt caggtgctga
     7801 ttttcttgga tcgctgatgt aaatctcttt gtgttcttta gaagctcttt catagccttc
     7861 ttttgcgatg aatgcttcta atttctcaaa cgttgctggt tcttcggcat agggcccgac
     7921 gtgtaagatt tgggcacaaa gaccttcttc catctttttg aatactagtt gttctttcaa
     7981 gtcttccgga agtttgctag aacgctgaat ggcttcttgt gctatcgctt ctgtaacaaa
     8041 gtcaggctgt cggatcatca attgataaga aaaatgttct tttttcatca ctggttcatg
     8101 caaatacttt tcttgcagtc cccattgtcc ttccagtgga tagacagtat acatctgata
     8161 atcaggaatc agccaatttt ttttaggcga catgcgtatt gcatagctga ctgcatatag
     8221 acaggctact cttcgtttaa agtcttcctg attcgggtcg ccgatccctc tgatgctaaa
     8281 gaatggtttt ttaggaacct ctagtatttg cgggcttttt ttcatactat agctcttgtc
     8341 tttacgccat tcaatttttt ccatttttct tcctccgcga aattccattt agttatttat
     8401 tatcatacct ctttttgagt gacttggcta atcgtgttga cactattttt cagtttgtgc
     8461 tagaataagt gaagtaaata aatcgtacag ataacacgtt tcggcgtgtc gagagagagg
     8521 cggtttctat ttttataata gaaatcactc tcttttttta cgaaattttt tagaggtgat
     8581 gtaaatgaca aaacaaaaac agctgctttt agtacctgca gtaattgcaa cagggatgat
     8641 gtcttttgcg ggcgtattga tcgagacggc catgaatgta actttcccaa ctcttatcag
     8701 acagtttgat ttaacgactg cacaagtcca atgggtaaca acgatttatc ttttgatgat
     8761 ttcgatcatt gttccgctgt ctacttattt gaatcgcaat tttagtctca aaaaactctt
     8821 tttagcttca aatctgcttt tcttggcagg tataatgatc gatttcttct ctcctacctt
     8881 catggtccta ttgtttggtc gattgctcca aggtgcagcg acaggtatcg ctcttccgtt
     8941 gatgttccat attatcttga catttgcccc cctcaatcga cgaggcgcga tgatgggtgt
     9001 cggtactttg acaacggcca ttgctccagc aattggcccc acttatggag gcattatgac
     9061 ttcacatttt acatggaatc atatcttcct gtttcttgtg cccgttttga ttctttcttt
     9121 gattatcggc tggtttgcta ttcccgagat ggcagtacaa aaaagtggtt atttggattg
     9181 gataagcgtt ttaggaattt gtcttttgtt tagtggaagt ctaacatttt tgagtacgtt
     9241 gggaacagtt tccagctgga ttgctttaat catcggcggt attggctggt tcctttttta
     9301 ctatcgaagt aaaaaagtgg agcatccact gatccgactg tctattcttt caaataaaac
     9361 attccgcttg tttttgattg gcttcttagt ttatcagttt ttattacttg gtgtatcttt
     9421 tgtcttgccg aatttccttc agatcgttca aggaagtagt ccttttgtcg ctggattagc
     9481 gatgcttccc ggcgcagcgg ttggtgcttt gctttctcct ttttccggga aaatgctgga
     9541 tcaatatggt ccgaagaagc caatttttgc tgggctgatt ctttctttga ttggttggct
     9601 agcattattc ctactcatag gacacgcttc agtgggtgta ttgattgctt gtcatgtgtt
     9661 ttatatgatc ggtatcggat tatcttacag caatatgatg accaccggaa tgaatgcact
     9721 gaatgaagaa ctacaaggag acgggaacgc aatgttcaat accttgcagc agttttctgg
     9781 cgctgtggct acttcattag tagcggtcat tatcaattac gtccagcatc atacttccca
     9841 taactatgaa gtgtctacta cattaggatc aaaagcagca ttaggcgtct tgttgctgtt
     9901 gcttctcgta agctttgctc gattcgccta ttatctgttc tttgctaaaa aagcgtagat
     9961 tccatatacg gcaggtgttg catctgccgt ttttttgtgt gaaaatggtt gtttgtaaaa
    10021 ggaggctttt ttatggaaaa aattgccatt atcggtggcg ggatcattgg tatgactcta
    10081 gccagttact tagatacatc aaaattcaat gttgctttat ttgatgaagg gacaggacag
    10141 gcaactaaag ctagcgctgg gattatctcc ccttggttgt cgaaacgcag aaacaaacaa
    10201 tggtatcgtc tggctaaaga cggcgctgct tttttcccta aacttataaa agatttccaa
    10261 cttgatcaaa cgatttacgc acaatcaggg acacttttgc ttcgacctga aaaagattta
    10321 gctgaattgg ctgttcttgc cgagaaacga aaagaagaag cgccagaaat tggaaacatc
    10381 gaatggcttt cacctgaaca gacatctcaa gcacttccgt tattgaaacc agcagtttca
    10441 ctgcgtatca gtggtggtgg tcgtctggat ggaaaagcat atttagaaca tcttggtcag
    10501 cgtttagcac aaaaacaaat ggagcagatc caggcaaaag tcactttgca taagaagagc
    10561 gattcttggc tcatcaaggg accgtttgga gaatacacag ctgatcgtgt aattttgaca
    10621 cctggcccgg cacttaagcc tttattacaa gaattaggct atgctgcaga tatacgtccg
    10681 caaaaagggc agctcttagc atttcagaca tcttataaga tgagtcaaga ctggcctgtt
    10741 gctatgcttg acggagaagc tgatttcatt cctttcatgg atggaaagat ccttttaggt
    10801 gctacccatg aaaacgatca aggctgggat ttggctccta ctagcgaagc ttataaggga
    10861 ttgactaaga gtacagaaca gttcttgact gatccagatc agataaaaaa ctggccatac
    10921 gagttccgtg taggtacccg tgcttatacg tcagattttt ccccattttt cggcgtactg
    10981 ccagatgatc caagcttact ggctgctagc ggtctaggct cttctggctt gaccacaggt
    11041 ccttatatcg gctatctatt agccaacttc ttaaatactg gtcattggtc tacggaagat
    11101 tatcaaaagg acatctccac ctatattacg aaaaatccat catagaaaaa cagctttttc
    11161 aagtaaggat cccgagcgat attgcgcatg gattacttct tgaaaaagct gttattttag
    11221 ttcaatttct gaatcaaatt ctccaaactt tcttttgctt catctggcaa gagatctttt
    11281 cctatctgag cttcaatgaa tcgtaaacga atatcaagtc gttgtttcac cagattttta
    11341 taggacgtat cctcaaaaga aacaggaaaa tctggtatag gtaaatagtg gatgtcttca
    11401 aatggaccaa acgacttgaa ttctgccgtt tgttgcacga ttctttcaac gattgacaat
    11461 gtcgtttcag gtttgatttt tttatcatcg aaatatccag tattcaggac atagcaggct
    11521 gtttgctgtt tcaaaaacaa ctctcgaaaa gcttgatagt cttccctaag aggatagaca
    11581 cggaaaggat tagcaaatgg ttcaatgact agctcatcta gctgtctttt tgtagtgata
    11641 ttttccgcgt ctgatcgttt tgttgctaat gttgcaccaa aagacgctgc taaaactggg
    11701 tcattgatct tgataattgg cggaagcgaa tcatctttca tgatccaaaa aacagcatcg
    11761 atcttttcgc taagatgatc gactcggtcg ggtgtcgcat aacgtgattt gactgtccgc
    11821 ccatttccgt ttcgtatatc tcctgttact aggacttttt ctcctttact tgtacatgtc
    11881 acgcccacat tctgacaagt aaggaaagaa gaaatggccg gatcatcaga cggatagtct
    11941 tgtgttttat caaagtaaga aggttccaag gcagtagcaa aaccattttt tcgagaaata
    12001 acgaaagcat cgtcatgcaa gatcgaaaca gagtgacctt ccgtatgttt tgctaaggta
    12061 atcgttgatt ttccagaacc ggacaaccca aatacagcca tcgtataggt ctggtcatta
    12121 ctagcatatt gtttcatccc accgtggcag gcaataaagc cattgcgatt tcccaatgcc
    12181 caagcaagcg tcaaagtcgc tttttttaat tctccaaagt aacgtaatcc tagcacagcc
    12241 gctgtattat ggagagcatc aaacaaaatg agtccatttg gaaatgtttc atgtgtccat
    12301 tcgggatcac aaaagatata aagatctccc tcatcaaagg aaacagactt ttgataagtc
    12361 tctaagatct tcgaattcgc tggctgaaaa ttcagcaaat aagaatacaa attgttctca
    12421 aatccttctg gaatcagcaa atgactttgc acagaaaatt cttcagatag gccaacgatt
    12481 acttttgtat ggtaaaactg gcgcttgcga ctttgataca cagcttctct taaaattttc
    12541 tcgtatttgc tttgttcctt gtctaagtcc tcaaagatcc gccgagcact tgctgttctt
    12601 cccacaatgc ttccatcatt gaatactagt gtcctaacat cagctggtaa atctagttct
    12661 tcagcatgtg agattgttaa atctgtgatg atcgttcctg gactcccttt tgctagttga
    12721 taagctgtct tcctgtcaaa aacttcatga acattattac cgtaaaatgt cgtttctaca
    12781 atcgatcgaa tactggataa cagtggattt ccacttgtga tttcgtttaa ttcgagactc
    12841 tcttttgtac tcatggctcc tcctcattcc ctaaacaaaa aaacgttttc tttattatac
    12901 attattcgag tttttatttg tactgctgcg ctgtgggaag caaacgagtt atttttcaca
    12961 caaaaaaacc gagaacttga ataaacaagt ttctcggttt catcattttc cttttatcgg
    13021 cttagtacca gccgtttgct aaccagaatg ttttagcagc atcccatgaa ccataacgag
    13081 atgttacata ttgttctgct acacgttctt gattggcagc tgaatagtcg ccgtttaagt
    13141 atgaagcatc taattgataa cggccgatgt aacgtccgtt agtagctgtg tatgaaccgc
    13201 ctgattcttt ttgtgcgatc cattcttttg cagagcttga agttggcgct gttgcagcag
    13261 ctggggcagc aggagctact tcaactgttt caacaggtgc aacgtatgta tgttcttgcg
    13321 ttgctgtttc agttgtagat gctgtatttt ctgttgtttt gttttctgtt gttgctgaag
    13381 aatcatttga agttgggatt gttaattgtt caccaacata aatgcggttg atgtttgcga
    13441 tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc tcctgcaaat ttttgtgaga
    13501 tcttagataa tgaatcacct gattttactg tataaacttc atcagcgtga gcatttgtag
    13561 ttcctacaaa cattgcagta gcaccagcag caaaagttgt acctaaaaca attttcttaa
    13621 atgaattcat attaaactcc tttttttctc cgttttttat tctttcgacg ctattaactt
    13681 tatcatttag aaatatttca taggtaaaag aaacatgaca tttaatgaca aaataacgaa
    13741 ttatttaata tttgtaacat ttaaacaaat atttttttga tacaaaacca taaaagcctt
    13801 gttgtataag caaagtaatc gtttttctat gtttttttat ttttgaatga aaaacgcaga
    13861 ttctgtaaca ttcactgcaa taatccataa aaaaaagcaa aaaaattttt gatttttatc
    13921 aaattctctt aaaaaaatgt aaaaaataca aaaaaaccag taattgttac aacaattact
    13981 ggacaaaaac tttgaataat gttaactatt tttttatttt aactttctcc gcaataattt
    14041 ctgggcatgt tccaattcgt ttataacgac ttcttctgtt tctttagcta atatttcatc
    14101 taatacatca agccattttt ccggttcttt tgttcctaga cgtccaatag cccaagctgc
    14161 tgttcctctg atgacagggc ggacatctgt taaacattct gaaatctcag gcaatgccgt
    14221 tcgatcacct agattagcta aagcaatcaa tgcgtttctc tgcaaaggtt tcttcccacg
    14281 ccatgaacca gctaaatgcc caaattgctg tttgaagtct ttattagaca tcgaaagcat
    14341 tggctttaat tttggatata cttcgtctac ttttggttcc atctcttcat gaaaatgaaa
    14401 atcttttcct tgattgtaag ggcagactaa ctggcagata tcacatccat aaatgacatt
    14461 tcgcattttt ttacgatatt cttccggcat catccctttt gtctgagttt gataagacaa
    14521 acatcgctgg gcattcattc ttccgtcacc aagcaatgct tttgtcggac aagcagtcac
    14581 gcagcgcata cagtcaccgc aaccaaattc tcctggttta tcaggctcaa atacaatatt
    14641 agtaacgatc tcaccaagat agacaaatga accgaactct tcagtgatca acaatccgtt
    14701 tcgtccaata aatcctaaac cagctcgctg tgctacagcg acatcgatca gttctccagt
    14761 atctacttgc ggagctagac gccattcctc ttctttttgc catttttctg ctcttgtttg
    14821 gatgaattcg atcaatcggt tcaaacggtc tctgagtata tcatggtaat cgatccccca
    14881 agaagcacga gcaaactgcc cgcgtttttc atccctaggc acttcttcat ggatcctagt
    14941 aggatacgca agtgcaatgg aaatgatcga tttcggctga tcaaatgtca tttctggata
    15001 aagacgctca tcgatatttg gatgttcaaa gccagaagta tggccagctg ctttttgttc
    15061 ttccaacgat tcttttaatg aatcaaaagg ttccgccgtg gtgaacccga ttttgtcaat
    15121 tcctaatcgt ttgctttctt gtatgatttc ttcttttaat gtcatggtct tcctcgcttg
    15181 ctgttaacgg ctgtataacc cataatatag tttttctttt ttcagctgga caaactggat
    15241 ctgctgatcg gcaatcttct tttcttccat ccattttttt cttgcagcaa atacttccca
    15301 ataatttgtt gaaattaatt ttcttttgtt atctaatgca ttaaaaaaat tcaatgaaac
    15361 tggttttgta cttaacagtt catggagcct tacctcttcc catggcgaaa actcagacag
    15421 atcgcgggaa tacaatactt cttcatagag atttttttgc tgtgtgtacg tgaatgtttg
    15481 cgggattttc ccaggcctag caatttctac tagacgatta taaaacatat tcattaattt
    15541 gctgttggca ataaattgac ttttataata ggccgactgg atctttttga ttactccttc
    15601 tgctttgatg actgaaaata atgcaggcca tgctttgacg gttatttcgt cgttttgttt
    15661 aactaatacg ccaggtgtga agccgactgg agaaaggaac acaaaataac cgataccggt
    15721 attttcatct aaaggctgaa attcatcaaa caaccatttg cctatgtaca gctcttcatc
    15781 tggttctgtt gtctcatttt gatttgctat gaataattta gctaattctg cttcaagaaa
    15841 ttgaaggtct tgatccttgt cggtattttc ctgctggctg atcaatcgaa agacttcttc
    15901 tttaatcgta tatttatctt tacgtgtaaa attcatagcc acaccttcct tccttactct
    15961 aactataacg gaatttcttc aaaaaaggta tctgtacgac gaaaaacagc ataatttttt
    16021 ttcatatctc ttataaaaaa tgcaaggtct gctcctcaag ctagaccttg cacttttcag
    16081 ctttcttttg tttgtgacaa accttcatgg atcgtttcaa tattccattt catcatctga
    16141 tagtaagaat ctccttgttc tccttctttt gctatggaat cagtgaacag tttatcatag
    16201 attttcaaac ccgtttcttt tgctacccgt tccatgcttc ttgagtctac actggtttcc
    16261 acgaataaaa ccggtacttc ttttgctctt atctgatcaa tgatcgcttt catttgatca
    16321 ggcgtacctt gactttctgt attgatctcc catatataag ctgctggcaa atcatatgct
    16381 cgtgaaaaat acttgaatgc cccttcactt gttactaata attttttgtt ctcttcgata
    16441 gaagcaaaac tttccttcgc ttgtgtatct aattctttta gtttttccac atatgcttct
    16501 gcattttttt tatagagtgt cgcattttct gcatcttttt cagagaatat acgtgcgatt
    16561 tcctccacat attttattcc gttagatagg tctagccaag catggggatc tgcttttgta
    16621 tgttcttcac cgctagttaa ataaagaggt tctacatttt tgctaactgc aaaataatct
    16681 tttccttctt cttttttagc cgtttccatc aagttatcga accagctgtt acctgtttca
    16741 agattcaaac cgttgtataa aataacatct gcatcgcttg cctttttgat gtcttctggt
    16801 agtacttcat attcatgcgg atctgttccg actggtacga tactgtggat atctatttga
    16861 tctgttccga cctcttttgc catgtccgcc aagatcgaat tggtagccac gactgacaac
    16921 ttttctttac ttccttcatc cgcttggtca gaagcctgct ttccgcaacc ggctaatacc
    16981 aagaaactaa ctgccaagat taaaaagagt gatttttttg ttttcatcta tctgcttcct
    17041 ttctgaaata aaattccttt tgttggagaa aataaaaaag caaggataaa aaatgcggta
    17101 catgctaaca cgatcgtagc tcctgatggc caattgtaac tataactgaa aaacacaccg
    17161 acaactgcac ttagcatacc gattcctgca gctgtaatga tcattttcag taaatggttc
    17221 gttagcaagt aagccgtggc ggctggtgtg atcaacatgg caatcaccaa gattgttcct
    17281 actgtctgta aactgactac agccactaat gtcaaaaaga acatcaaaag ataatggatc
    17341 agcgatgtgt tcagcccata agcttttgcc attgtcggat cgaatgatgt gatcttcaat
    17401 tctttataaa agaaaaagac aaatatcaag acgattccac taacgagtgc tgtcagcaac
    17461 aaatcgcttt ccctaacagc taagacattc ccaaataaaa tatgatacaa atctgtcgca
    17521 ctctgggcat atgaaatcaa gataatccct aatgcaaaaa aggagctaaa gacaattcca
    17581 atcgctgtat catttttcaa tcggctccgc tgagtcacga aaccaatcac tgctgctgtt
    17641 agcatcccaa aagcagtggc tccaaagatg taactaaaac caaacatata agaaatggct
    17701 acacctggaa gaactgcgtg agagatagcg tctcccatca aagacatccc tcgtaaaata
    17761 ataaaagaac cgatgactcc agaagcaagt cctacaagca tggaagtgac caaagcgtat
    17821 tgcaggaatt gataattcat taatccatca ataaaggatt ggatcatttt ttctctcctc
    17881 cttttactaa atgtctgatg atctcaccat aggccaattg aaggttcttc gatgtaaaga
    17941 cttcttgtac tggaccggaa gcgatcagct gtttatttaa aagaataacc tcatcgaaat
    18001 attcttctac tttatgaaga tcatgatgga cgatcaaaat cgtttttcct gaattcttca
    18061 attcctgcaa gatgtcaaag atctttcgtt cacttaacgc atcaatccct acgaatggtt
    18121 catctaaaaa gatccattct gctccttggg ctagagctct tgcaataaaa actctctgaa
    18181 gctgtccacc cgatagttcg ctgatctgtc ttttggcata ttcttccatc cctacttttt
    18241 tcaaagcttg tcttgcacgt tctttttctt ttttcccagg tctttgtcca attcgtaaag
    18301 atggatatgt tcctaaaagt acaacgccta atacatcaat tggaaaggaa agatccaatt
    18361 cacttcgctg ttccacgtag gctatttttt tacggactga gtttaccggc tgtccagaaa
    18421 aggtgacttt accagtagta gctggtatca actctagcaa agatttcata aatgttgatt
    18481 ttcctgcacc gtttggtccg ataataccgg taattttttg tggatttatc gtcaagttga
    18541 tagttttcaa aacagttttc ccttgataag cggctgaaag gtcttgaatt acaatggccg
    18601 tacgttccaa atcttacact cctttttagg tttacctaac tttttattta aatatagttt
    18661 aactaaaatg gaaagcattt tcaagtagaa tgatgaaaaa gtttttttcg aattttcctc
    18721 gcattttatg aaaattttct gtagaatcac atcaagagtt caataaggct aacctaataa
    18781 tgaaaaacat gtgttgaaac gtttgattat tttttcttgt tgactcaaca acgaaaggag
    18841 tttttgtatg gaagaagaaa agttaactcg aagtcttagt gcccgtcata tccaaatgat
    18901 cgcactaggt ggaacgatcg gtgtcggtct gtttatgggt gcctcctcaa ccatacgttg
    18961 gacagggccc tcagtgatgg gagcctatgc gattgcagga ttctttttgt atttgattat
    19021 gcgtgcttta ggcgaaatgc tttacgttga tccttcgact ggttcttttg caaattacgc
    19081 gagcgagtac atccatcctg tggctggcta cttgaccgct tggagtaata tttttcaata
    19141 tattgtcgta ggaataagtg aagtgattgc tgtaggatct tatatgaatt attggtggcc
    19201 tgatttgccg gcaattattc ccggaatcat tgtcgtcttg tttcttatgc tggcaaatct
    19261 tatttctgtc aaggcgtttg gcgaattaga attttggttt tcgatcatca aagtcataac
    19321 gatcattttg atgatcattg caggtcttgg ggtaatctta tttggatttg gtaatcatgg
    19381 tcaggcagta gggatttcaa atctttggaa aaatggtgga ttcttcactg gaggcgtcaa
    19441 aggatttttc tttgcattat cgatcgttgt tgcttcctat caaggaatcg aactgatcgg
    19501 aatgactgct ggtgaagcag agaatcctaa aaagacgatc attgaagctg tccagtccac
    19561 tatcggtcga atcttgattt tttatattgg tgctattttt atcattgtga gtatctatcc
    19621 gtggaatcaa ttgagtgaga ttggttcacc ttttgtacaa actttttcta aagtcggcat
    19681 tacatttgct gcaggactga ttaattttgt cgtgattaca gcggcattat caggttgcaa
    19741 ttcagggatt ttcagcgcaa gtcgtatggt ctatacactt gccataaatg gaaagatgtc
    19801 taaaaaattt ctgaaactat ctcgtcacgg tgcgcctttt tatcctgtcg tagcaatctc
    19861 aggtggaatc ttaattggtc tgatcttaaa ttatcttcta ccaatgttca tcaaccagtc
    19921 agaaaatatt ttcgttttcg tatacagctc aagtattttg cctggtatgg ttccttgggt
    19981 agtgattttg atcagcgaga ttcgcttcaa aaagaagcac gcagataaaa tgcatgatca
    20041 tccttttaaa atgccactat ctccttatac gaattatgtg acacttgctt ttttagctat
    20101 cgtattgatt ttcatgttta tcaatcctga aacgagaatc tcattgatta ttggactcgt
    20161 tttcttgctt tacatgacga tccattactt tgtacgcgaa aaaagaaccg gaaaataaaa
    20221 aaagaacgaa gcttcacctt agtggaagct tcgttctttt ttattgcttt tatcaattca
    20281 attttagcgt ctcaccggga gtcaggaaat aattctccat gctcatccca ttcagcgaca
    20341 agatcgtttc tactggtacg ccagttcgtt cggctatttg atttggacct tctccttcca
    20401 aaactacggt tgtcgcagcc tgtgaagtcg cgctgttggt attagtttca gaggaatctg
    20461 ttgtgtcttg tgttgttcga gcttgcattt cagaagcttc taccgactct gctgtctcag
    20521 aatcagaaga tgttgtgtct tgtgtgcttg ttgactctgc ttgaagagag gcgctagtct
    20581 gcaaataaat atcggaaatc ttagcgacat gattcgtata attagccgat aattgattga
    20641 tcaaagtatc taagtctaca ccgtctttat tttctgctgc ctgatacgct gcacgtaatt
    20701 ttctgatccc ttcgtcggag attgcttgaa gctcacgtgc tttttggtta gcaactgctg
    20761 ataaaccagc tattccctct tgatttccct gaatttctgt ctggtattct tcaatcaatg
    20821 caggcgtctt ttcttcgatc tttttcgaat aatcatttac gattgcttga agttgctctg
    20881 gcaaaccatt tgctgttttt tcttcagaag tttgtgtctc ttttgtttta gttgatgact
    20941 cacttgtttg tttcgtctct gttttctttg tcgttgtttc cgttatggtc gtacttttcg
    21001 cttctgacga attttttcca tgaaaatata gacccgcaac tcctcctgct atgatcaaaa
    21061 acacggcaac tgctgcccca atcatgatca tccgcttacg agctgcctgt ttttgctgtc
    21121 ttcttcgggt catccgaaca ttttctcccc actcattttc atcatacatt tttttcacca
    21181 ccaattttta tgtataatag cgttatcaaa cgcttcatat tcttattgta gcaaataatt
    21241 gtcgctgtgc acatttttat ctttgttatt tataagaaaa tttcaccagt tgagatgatt
    21301 ccaagattgt ccgtcgattt ctccttgcag ctgcattgtt cgttcgtcta tctcagccat
    21361 cagtttcttt atttcatata agagcgcacg gtcttgatat tctgattttt cgcttaattc
    21421 atctaattgt tggatcagcc aatcatttgt cataatgaag ttcctttcat ttcttgcagt
    21481 gtaaaggtca agtcttcgat ctgctctttt gcctcagtca gtgcttctac gatatatgct
    21541 tgtttttcag attcttcttc ctgcattttc tgattttcct gtactctttg aataaaccag
    21601 tcaatccgct gagtcagcga tggtttgcct tccaatgaga gatactgctg ataagcaatt
    21661 agcttctccc aacgtttttc ctgggataaa tacataaatt gtgctaaaac gaatggcgga
    21721 agataggtca tatggaattt atttgcgatt cgctgatacg gcctcaaaaa ttcggaaatc
    21781 gtatattctt cttttcctcc agcttggtat tctttttccg ccacacctgt tgtcaatacg
    21841 attcctagtt ctttcccttt taatacatgt gctgctcgtt ccaagacatt atcttgccaa
    21901 atttttaaat gagcaggaga actataccag taaaacggaa actggaaaat gatccgatca
    21961 tgctgggcca agagctgtag ttcagtctct atatctattt ttccgtctgg ataacttgat
    22021 tccaaatgat gccaagtagc tggtaaacca gcagaagctt ctttgaaaaa ttgctgattt
    22081 aaagattctt cgatcgttgg atggctaatg atgataagtg tacgcatccc ttttcctcct
    22141 ttttagtctt cgctttacag tgtagcataa ccgccacaag tttcttttgc cgatgcttgt
    22201 cattttaacc agactaatca ggttgtccaa gaaactgcgg tttgtttttc caactgtttt
    22261 ttcaaggtat ccaaagcaat attcttaaaa ttttcgtttt tcatacgttt acgatagttc
    22321 gtagccgttt ctccggtact ctttttgaac attcggctaa atacgactag actattgaac
    22381 cctacttgat aggcaatact ttccacggat tcatccgtca gagaaagaag aaacgcccct
    22441 cgattgatcc gtacctcgat caaatattcc ttgatactgg tacccatatg atttttgaat
    22501 actcgcgaca aatggctccg attgaccgct aaatgctggg cgatttcctc aacagacaat
    22561 ttttcagcat aatgtgcctc gatgtatttg actgtttcaa cagctaaaga ggaatgaacc
    22621 ttttgtacac caccgctgac ctttccgcca tcttttaaaa ggactgataa aaaacggtag
    22681 gttaatgctg ttaactgtaa ttcattttcc aaggtttctt ccgtatcttt catgcattct
    22741 aaaataatgt cgatatattt ttgtatgtca gaagagacca tcgtgtattg gtcttctctg
    22801 aaaagcgact gccgaatgat ttcttctgca gcactcccac caaatgaaag ccagcaatac
    22861 atccacggat cttctccatc tgataagtaa aacgtcgagt ctcctggacg aatcaagaac
    22921 agatcacctt ttttcaattg gaattgttga tttttcacat gataagatcc tttgccttcc
    22981 atcacgacat gcaggatgta atcattgcga atagcaggac caaaagaatg aaacggcatc
    23041 gttttagaga aaccacaaaa atcaaaatac aattcttttt ctacttgttg atgttccttt
    23101 tttaaaaaaa tcgattgctc cacgtctatc cctccttact cctttagtat aatgcaacaa
    23161 aaagttaaat aaaagtcaca gaaatatatt aaaaaggaag cgatttcttg ttaagtttaa
    23221 gtcattaaaa gatctttgtt attcgttgta accggtttac ttagtattat atattgaaca
    23281 ggagaggaat acagattatg aaaagcaaaa tgatccaacg tgtgtcctac gctttaggcg
    23341 cttttggaca cgacacattc tatgttacat taagcactta ttttatggta ttcgtgacaa
    23401 gccagatgtt cgcaggaaca gataaggcaa cgaatgcaaa aatgattgct acagttacaa
    23461 gcttagttgt aggtatccgc ttagttgaaa ttatttttga tcctttgatt ggtggcatca
    23521 tcgataatac tcgtacaaaa tacggtaaat tcaaaccatg gttagttatc ggcggcgttg
    23581 ttagttccgt actcttggta gcaatcttta ccaatttctt tggcttagct actcataata
    23641 cgactttatt tatcatatta tttgcaatcg ttttcatctt attggatagt ttttattcat
    23701 ttaaggatat cgctttttgg tcaatgattc cagctttgag tacggaaaca aaggaacgtg
    23761 aaaaatttgc tacgtttgct cgtttcggct cttctctagg tgcaaatggt actactctag
    23821 tcgttgtccc tatcgttact ttctttacct atcttgttac tcataaacat attgaaggcg
    23881 cttcaggctg gttctggttc ggtgtgattg ctgctgttat ttcaggcggt actgcgatta
    23941 tcactgcctt gggcacaaaa gaagtcgata ctgcgatccg tcagcaaaat gaaaaagctg
    24001 ggatcaaaga tgtgttcaaa gcagttgctc aaaatgatca gttgatgtgg cttgcccttg
    24061 cttatatcgc ttatgcgatt gccaatgcag caacaactgc cgtattattc tacttcttca
    24121 aatatgtact tggccaacca actgaatttt ggatcgttgg tctagttgct gccttacttg
    24181 gtttagtagc tgttccacta ttcccaatgc ttacacgcgt catctctcgt cgctacgttt
    24241 accttggagg gatcgcaatg atgatttctt cttacttttt ctttacgatc gcaggttcaa
    24301 gtttgccaat cgttatcatc gggctagtat tgttctactt ccctcaacaa ttgattttct
    24361 tatccgcttt gatgacgatc acagactctg ttgaatatgg tcaatggaaa aatggtatcc
    24421 gtaacgaagc tgttactcta tcaattcgcc ctttactaga taaaatcgct ggtgctttgt
    24481 ctaacgggat cgtagggttt gtcgctatcg cttctgggat gactggtaat gctactgctg
    24541 ctgatatcac tgctcatggc gtaacgactt tcaaagctta tgcattctat gctcctgctg
    24601 ctttgatgat tttctccgct attatttttg cttggaagat tacattgaca gagaaaaaac
    24661 atgcacaaat cgttgaggaa ttggaaaaca aattggctcc agctgataca atggaagatg
    24721 aacttcacga tgcagtagtt ccttcccatt aagaaaggat cttgaaaatg aagattacag
    24781 aaaaagattt tggccaaggt tatcacttga taaccttgga gaacaaaaac aagctagcac
    24841 tttctatctc tgatttaggt gctagaatcg tcagtttaaa atcaaatgat cgagaattag
    24901 ttttgggatt tgacaccgct gaagaatata tcgaaaaaga tccgtatatc ggtgcttcta
    24961 tcggtcgcac cgcagggaga atcgaaaatg gccgcttttc tctcaatgga aaaacttacc
    25021 aattagccac agatccaaaa actggacata atctgcatgg tggtgctcct ggatttgaat
    25081 taaaaaaatg gtcttatgtc attttgaatg gcgaaaatga agcttctgtc atttttacta
    25141 ctacaagtcc cgacggagaa catggttttc caggtacaat ggatgtggaa atccgctata
    25201 ctttgacaaa agataatatc tggcgagtga cctctcgcgg caccagtgac caagatactt
    25261 tattcaatcc gacaaatcat gtttacttca acttgacggg agatgcttct cagtcaatcg
    25321 atcaacatga gctttggctc aatagcgaag cttatgctcc tttgcgaaca gatagtattc
    25381 ctataggggt aaaagaaaat gctgcaggaa gtgctttcga ttttcaaata cctaaaaaat
    25441 tagcgtccgt ttttgctagt gatttggatc aaaaaaatct tgttgacgga atcgatcatc
    25501 catttttctt aaaagaaacc ggcttgggta aagaagccgc tcgcttgact agccccgata
    25561 aaagaatcca agtaggtatt gctacagatg cttctagtgt ggttattttc actgcaaatt
    25621 tcggcacaga aacaccagaa atgcgcaacc gaaaactggc acatcacggt ggcatcacat
    25681 ttgagactca aacagctcca ggtgcagagc gcttctcagc atttggttcg attcatttaa
    25741 aagctggatc agtatttgaa acagtgacag aattcaaaat caaaacaaga aaggaataac
    25801 tcatcatgga aatcaaaaga agcttaaaca caaaatttgc cgaaacattc ggacctaaaa
    25861 aaactacaca atacttctca ccaggacgca tcaatttgat tggtgaacac acagattaca
    25921 atggaggcca tgtcttcccc gcttccatca cttatggaac gcaaggtgta gcagcacctc
    25981 gagacgataa taaaatatta gtttattcca caaactttga cgatatcggt gtcattagtt
    26041 ttactttaga tgagctagaa tatgataaaa aagcaaactg ggcaaattac gtaaaaggaa
    26101 tggtccttaa attaaaagaa gctggacact caatcgatca tgggtttgag ttgttagtgg
    26161 aaggaacaat ccctaatggt gcaggacttt ccagtagcgc ttcattagaa ttattagtag
    26221 gtgttgtatt agaagatctg tttgatttaa aaacagatcg cttggaacta gtccaaacag
    26281 gtaaaaaagt agaaaatgaa ttcatcggtg tcaattcagg tatcatggat cagtttgcca
    26341 tcggcttcgg tgagctagat catgcaatct tattagatac aaatacgctg aaatacgaaa
    26401 tggttccagt gaaactagat ggttatgcaa tcgtcatcat gaatacgaat aagcggcggg
    26461 aattggcaga ttcaaaatac aatgaacgcc gtagcgaatg cgaagaagcc ttggcacgtt
    26521 tgcaaaaaac tttgcctatc tcttctttag gagacttaga tgaagaagaa tttgaatcaa
    26581 ctattgacca aatcggcgat gatacattga tacgcagagc tcgccatgcc gtttctgaaa
    26641 atcagcggac tttgaaggca aaagctgaat tagaagctgg gaacttagaa gcatttggtc
    26701 aactgttaaa tgattctcac cactctcttc gctatgatta tgaagtaact gggatcgaat
    26761 tagatacact agtagatgct gctcaaaaac aagaaggtgt cttaggtgca cgaatgacag
    26821 gagctggatt tggtggctgt gcgattgctc ttgtcaaaga agaaaatata cctgccttca
    26881 aaaacaatgt ctatgatgaa tacatgaaag ttgtcggcta tgctccggaa ttttatgttg
    26941 cacacattgg aaatgggaca acaaagatcg atccagaagc tgttcatgtt tgataaaaaa
    27001 atcaacgatc tcagctaaaa acaaatagat aaaaataacc tctcagcaat ttccaaatgt
    27061 gagccttttg ctcgtatttg aaaattgctg ttttttattg acacggaaag agaacgtgtg
    27121 ttctaattgt gttgaagagg tgattgttat gtgggcgaat caaaatccgg tatttgttta
    27181 tgaaaaagaa ccttcaagag atattttatg tattgactgc aaatcttttt acgcctccgt
    27241 agagtgcgtt gaaaaggatc ttgatcccct tacaactaaa ttagtagtca tgtcctatcc
    27301 gtccgattcg acagaaacac ggggtagcgg attaatcttg gcttccagtc ctgcagcaaa
    27361 aaaagcttac ggcatcacca atatcagccg tgccagagat cttcccttcc cctatccaag
    27421 tgatttatat attgttgccc caaggatggc ctattatatg gaaaagaaca gagaaatcaa
    27481 ttctatctat aaaaaatatg cagacgaagc gaaccatcat gtctattccg tcgatgaaag
    27541 cttccttgat gtaacaaata gcatggcact ctttggtaaa acaaatgcgt acgacatggc
    27601 taaactgatc cataaagatg tgtttgagca gaccggaatc tacacgacta taggaattgg
    27661 cgataacccg ctacttgcta aactagcctt agacaatggc tcaaaagacg gaaagcaaat
    27721 gtttgctgaa tggcgttatg aagatgtacc agaaaagtta tggaaaatct cagatatcac
    27781 tgacttttgg ggaatcggca aacgaacagc aaaacgtctg aaacaaatgg gtatacattc
    27841 tgtttatgat ttagctcacg cggattatta tcacttgaaa agcaagctag gggtcatcgg
    27901 tactcagctt tatgcccatg cttggggcat cgatcgaagt tttttgggac aagaatatac
    27961 accaaaatca aaaagtatcg gaaatagtca aatcctagat agagattata caagacgaga
    28021 agaaatcgag atcgttatca aagaaatggc cgaccaagtc ggcactcgct tgagacgatc
    28081 tgcagcaaaa acacaagtag tcagcttatg ggtaggatac tctttaggct atcttgatcc
    28141 aaatggcaag accgggtttc accagcaaat gaaaatcgaa gcgacaaatg ctagcagaga
    28201 tctagcagat gctttattga ccattttcca ccagcattac cactaccagg atattcggcg
    28261 tgtaggcgtg aactgttccg acctcgtata cgcgacaggt ttacagctta acctttttga
    28321 agatcctgaa aagcagataa atgaagcgaa agttgatttt gttgtcgata cgattagaaa
    28381 aaaatacggc tttcaatcaa tcatccatgc gcatagtctc ttgcaaggag gacgcgccat
    28441 cgcacgtagt tctctcgttg gcgggcatgc aggtggtatg agtggattag aaggtggaaa
    28501 agatgagacg gacagcaaaa aaattcattg actataacga gtatcgagat cggccatttg
    28561 gattaaaatg ggggactgct tttgcgatgg aagaactagt caacggtatc aaaaaaaatg
    28621 aagaagaagc acgcaaacag cctcttccta aacaacaaat gacattgcaa gaaatcgata
    28681 gtatactgac agaagccttt ttatctcgta aagcagtaat cattcagctc aatttaaaag
    28741 atgaatttgg aagactcttg gatgaggtcg aagggcactt cattggtgaa gcctatgaag
    28801 attactttat gattgaccat cagccgatat tgtgggaaga cgtacggtat attgagttaa
    28861 aggaaacaaa aaaatggttt gtgacatgag aggttgtgac agaagtgatc aacttcaaaa
    28921 aataagaaag gatttttcga aaattgcttc ttaaattttt gtggaatttt ggcttatttt
    28981 tcgaagaagc aacttctgga acaacgtata ttagatatca gaggttgtga aaaagtcgct
    29041 tagttttgag taacaagcca aggcgctgat ttcacaacct ctgtctatct atccatctat
    29101 tcggtaagat tcatgcatga cgtcttcttt atttagtatg tagagccctt tttcttctga
    29161 ccattcaaca taaatcagct gttttacatc tgcatagcct tctttcttca agttctccat
    29221 aagccatgct tcagtcaatt gattggactg aagcacttta tgctgaattt gcccttcatc
    29281 tacaagcagg atcgataaag ctttgtcttc atctgatttt ttcaatacac tcatttgacc
    29341 agcattctct aggataacat gtttggcatt ttcaagagaa aagcattctt gctgtcttaa
    29401 gattgctcgc aactgctcca tctcgatatg gttatttgtc aactctgtca tgttgatgac
    29461 gccgtctttg attagaacag atggctcgcc tttgacccaa cgattgacct tttcattttt
    29521 ttggacgata cgttcgatac cataaatcat tactgcccaa agcgcaatgg caaataggac
    29581 atgccctaca tggacattgt catcataggt tgattcttct aagatcccac ctaaaacaag
    29641 cgtatagact aaatcaaacg gtgtgatttc tgacatggat ttcttcccaa gcaaacgtac
    29701 cactaataat aaaccgatca acccgaacac cagtttcatt gtaattgaaa aaatcatttt
    29761 ttctccccct aactatctgt taattaaatt atatatgctt tctcacgaac acgcatggaa
    29821 tatacaagga agaaatgtat ccttttacta aaaaaaggac tgtgacactg tagtgacccc
    29881 caaaagttag acttttttgg agtggagaaa tccactcctt ttttttatgc tctaaaatga
    29941 tatttttaca caaccgttac ggttgtttta cgaaattgga cg
//
LOCUS       DANJLF010000030        27869 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000030 DANJLF010000000
VERSION     DANJLF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="PWT25_001461"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="PWT25_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL1021553.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="PWT25_001462"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="PWT25_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL1021554.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="PWT25_001463"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="PWT25_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL1021555.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="PWT25_001464"
     CDS             1640..2272
                     /locus_tag="PWT25_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL1021556.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="PWT25_001465"
     CDS             complement(2274..3179)
                     /locus_tag="PWT25_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL1021557.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="PWT25_001466"
     CDS             complement(3264..3923)
                     /locus_tag="PWT25_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL1021558.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="PWT25_001467"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="PWT25_001467"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL1021559.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="PWT25_001468"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="PWT25_001468"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL1021560.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="PWT25_001469"
     CDS             6166..7251
                     /locus_tag="PWT25_001469"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL1021561.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /locus_tag="PWT25_001470"
     CDS             7248..8312
                     /locus_tag="PWT25_001470"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL1021562.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="PWT25_001471"
     CDS             complement(8561..9364)
                     /locus_tag="PWT25_001471"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL1021563.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="PWT25_001472"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="PWT25_001472"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL1021564.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="PWT25_001473"
     CDS             complement(10242..11249)
                     /locus_tag="PWT25_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL1021565.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="PWT25_001474"
     CDS             complement(11256..11900)
                     /locus_tag="PWT25_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL1021566.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="PWT25_001475"
     CDS             complement(12000..12758)
                     /locus_tag="PWT25_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL1021567.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="PWT25_001476"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="PWT25_001476"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL1021568.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="PWT25_001477"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="PWT25_001477"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL1021569.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="PWT25_001478"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="PWT25_001478"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL1021570.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="PWT25_001479"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="PWT25_001479"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL1021571.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="PWT25_001480"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="PWT25_001480"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL1021572.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="PWT25_001481"
     CDS             18156..19025
                     /locus_tag="PWT25_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL1021573.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="PWT25_001482"
     CDS             complement(19151..20050)
                     /locus_tag="PWT25_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021574.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="PWT25_001483"
     CDS             complement(20215..21039)
                     /locus_tag="PWT25_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL1021575.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="PWT25_001484"
     CDS             complement(21050..22345)
                     /locus_tag="PWT25_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL1021576.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="PWT25_001485"
     CDS             complement(22361..22840)
                     /locus_tag="PWT25_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL1021577.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="PWT25_001486"
     CDS             complement(22846..23874)
                     /locus_tag="PWT25_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL1021578.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="PWT25_001487"
     CDS             complement(23891..24997)
                     /locus_tag="PWT25_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL1021579.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="PWT25_001488"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="PWT25_001488"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL1021580.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7857 a         6124 c         5035 g         8853 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacat
//
LOCUS       DANJLF010000031        26859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000031 DANJLF010000000
VERSION     DANJLF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(362..1144)
                     /locus_tag="PWT25_001489"
     CDS             complement(362..1144)
                     /locus_tag="PWT25_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021581.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1209..2045)
                     /locus_tag="PWT25_001490"
     CDS             complement(1209..2045)
                     /locus_tag="PWT25_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1021582.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2063..3472)
                     /locus_tag="PWT25_001491"
     CDS             complement(2063..3472)
                     /locus_tag="PWT25_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1021583.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3486..5309)
                     /locus_tag="PWT25_001492"
     CDS             complement(3486..5309)
                     /locus_tag="PWT25_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1021584.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6152..6478)
                     /locus_tag="PWT25_001493"
     CDS             complement(6152..6478)
                     /locus_tag="PWT25_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021585.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6524..6886)
                     /locus_tag="PWT25_001494"
     CDS             complement(6524..6886)
                     /locus_tag="PWT25_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDL1021586.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6923..7138)
                     /locus_tag="PWT25_001495"
     CDS             complement(6923..7138)
                     /locus_tag="PWT25_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1021587.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7265..7600
                     /locus_tag="PWT25_001496"
     CDS             7265..7600
                     /locus_tag="PWT25_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1021588.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7676..9673)
                     /locus_tag="PWT25_001497"
     CDS             complement(7676..9673)
                     /locus_tag="PWT25_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021589.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9771..10046)
                     /locus_tag="PWT25_001498"
     CDS             complement(9771..10046)
                     /locus_tag="PWT25_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021590.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10278..11012
                     /locus_tag="PWT25_001499"
     CDS             10278..11012
                     /locus_tag="PWT25_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1021591.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11051..12193
                     /locus_tag="PWT25_001500"
     CDS             11051..12193
                     /locus_tag="PWT25_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1021592.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12288..13679)
                     /gene="sufB"
                     /locus_tag="PWT25_001501"
     CDS             complement(12288..13679)
                     /gene="sufB"
                     /locus_tag="PWT25_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL1021593.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13684..14154)
                     /locus_tag="PWT25_001502"
     CDS             complement(13684..14154)
                     /locus_tag="PWT25_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL1021594.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14141..15376)
                     /locus_tag="PWT25_001503"
     CDS             complement(14141..15376)
                     /locus_tag="PWT25_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL1021595.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15373..16659)
                     /gene="sufD"
                     /locus_tag="PWT25_001504"
     CDS             complement(15373..16659)
                     /gene="sufD"
                     /locus_tag="PWT25_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL1021596.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16672..17445)
                     /gene="sufC"
                     /locus_tag="PWT25_001505"
     CDS             complement(16672..17445)
                     /gene="sufC"
                     /locus_tag="PWT25_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL1021597.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17642..18490)
                     /locus_tag="PWT25_001506"
     CDS             complement(17642..18490)
                     /locus_tag="PWT25_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL1021598.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18514..19200)
                     /locus_tag="PWT25_001507"
     CDS             complement(18514..19200)
                     /locus_tag="PWT25_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021599.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19193..20227)
                     /locus_tag="PWT25_001508"
     CDS             complement(19193..20227)
                     /locus_tag="PWT25_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1021600.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20290..20546)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20622..20960)
                     /locus_tag="PWT25_001509"
     CDS             complement(20622..20960)
                     /locus_tag="PWT25_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL1021601.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20962..21318)
                     /locus_tag="PWT25_001510"
     CDS             complement(20962..21318)
                     /locus_tag="PWT25_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL1021602.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21324..22511)
                     /locus_tag="PWT25_001511"
     CDS             complement(21324..22511)
                     /locus_tag="PWT25_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL1021603.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22702..23547)
                     /locus_tag="PWT25_001512"
     CDS             complement(22702..23547)
                     /locus_tag="PWT25_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1021604.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23781..24746
                     /locus_tag="PWT25_001513"
     CDS             23781..24746
                     /locus_tag="PWT25_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL1021605.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25123..26166
                     /locus_tag="PWT25_001514"
     CDS             25123..26166
                     /locus_tag="PWT25_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL1021606.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26228..26692)
                     /locus_tag="PWT25_001515"
     CDS             complement(26228..26692)
                     /locus_tag="PWT25_001515"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL1021607.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
BASE COUNT         7816 a         5619 c         4600 g         8824 t
ORIGIN      
        1 tagactgatc gttaacttat tttttatatt tctacatagt tatctttaga accatttcag
       61 atacatatga aacaattaca tatgattttt tcataaaaaa acagtactgt ctagtagtat
      121 aaaatataca aatttcttaa aaattttttc actaaaatca ctacctgaaa cagtctaaaa
      181 cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac aaataaatat
      241 agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc tactggagat
      301 gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga tggaagaatc
      361 attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg ttcttaaata
      421 ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatgtct ttaccaactt
      481 gctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat aaagattgag
      541 catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca tttacaagct
      601 tccaagtagg tggtatagct gtgtatgttt cgatgaatga atcaattctt tcatctatcg
      661 tcttcccagt atctacttct gtctcattac tcttagatga atcgattctt tcatccatcg
      721 tctgctccgt aactgcttct gtctcattat tctcaccgga aactctcttc acacccaaac
      781 ttatatattg tccatcttta gtgtgcatag ttaaaattcc cacgctttta ctaataaatt
      841 cactataacc agttctttca atagagactt caatgtattt cacactatca tactcaaaag
      901 aaaaatagac atgtaaaagc attttgttgt ttacatctac ataataatcc tttgactcta
      961 cttgagacct tcctatacgg aatacagctt tactgtctga tatctctata ccagcatcct
     1021 tattattaat ctgccaatct ccaagtagct cttctggcaa gctagtagtt tcttgtgaac
     1081 actttaccac ttcactggta gaggtcgaaa cattcacctc cgcactgtta gaagaatttt
     1141 ccaaggaatc aggagcctta gcacaaccaa caagtcttta tatattgtgc agccgatatt
     1201 ttcttcattc agtacaccct tgaaatatgc agtgttagat aaagcagttc gtcctgattt
     1261 gctgaggcac ccatctcttc ttgaacgtac tccttgattt tcaaggccgc ccgatactct
     1321 ttcggaaaac gctgcttcac tgaagccagc aaaaaatcat ccttctccgg caactcttcc
     1381 ttcttaatca ttcgggcaat aaaaaactgt aaatgcgtca caaatcttaa gtaaagcatc
     1441 gaatctttat caaccttttt ttcgggaaac agatatccta gcatagccaa gatatccttt
     1501 agtgctctta ccgagagcat ggtttcttcc atatttgctt gttcactttg ggcatttaca
     1561 atgtgaaacg caatgtttcc agcctcctct tctggcaact gaacattcat ttttttgttg
     1621 atcaattgga tagcatagca gccgatctca aattcttttg ggtaatactt ttgaatttca
     1681 atcagcaggc gattttgaaa aacaatgcct ttttgactgc gttcaatggc atgcttcaag
     1741 tgatcaacca aggtaaaaaa tagactttta ttcagcttaa agtcaaactt ggcgttggca
     1801 taatcgataa ttcgcttgac gcaaataatt agatcatccg gagcagcctc aaacagttta
     1861 atgtaatcct tttttagagc atcagaaagt gaatctaatt taaatacttt ttggattttg
     1921 tcagccggaa ccaaatcgcc tttctttagt tgaaatccaa tccctttccc catcaaaatt
     1981 acttctttgc cttgcttatc taagcaaaga attaaactgt tgtttaacgt tttaattact
     2041 ctcattagat tcaccgttct ctctaagcta agccatttct tgcgattact ttttgatacc
     2101 aaaagaagct ttcttttttt attcgtttta attctttaag attctcatcg gttcggtcaa
     2161 catatataaa accataacgc ttattgaagc cttgatgggt actaacaaca tcaatcgctg
     2221 accaaggtaa ataaccaaaa acatctacac catctgttaa agctgcttga atagccagaa
     2281 tatgtttggc tagataatca attcgatcct ggtcattaat tcgtctatct tcctcaacta
     2341 tgtcgggtgc cccatagcca ttttcagtaa ttaaaatagg taaatcatag cgatcgtaca
     2401 atttcctcaa tgttaatcgt agtccagtag ggtcaactac ccagccatat ggcgttttat
     2461 ctacataagg attttcagct gcccggtaga caccttcttc tcccaacatg atctgttgat
     2521 cccccgctct agcggaaaca tctgatccgt catttttgct ggcagcgatg gtggcagttg
     2581 aataataatt aatcgcaata aaatcgggtt taccggagga taaaattgcc tgatcctctg
     2641 cctcaatttt aggatataat ccacgatcat tcaaatagtt ttcaaacaaa taattgtatt
     2701 ttccccgaac tgctacatct aagaaagacc agcctctcaa tgtttcccaa ttatgggctg
     2761 caattgcatc ttctggactg gaggtagctt gatacatagc cgttaaattg attgctggac
     2821 cgatttttcc tttaagattc aactgatgat acaagtgaat tactctagct tgagctaata
     2881 aaacatggtg attaatttga aagagttctt tttttgaagg cagctctcca cttggtggca
     2941 aaccaatcgc tccaggatga aggatcatcg tgttttgctc attaattgtc agccaatagt
     3001 tcaccttgtc accatactca gtaagcaata cctgtacata gtccacaaaa taatcgatag
     3061 ttgctcgatt taaccaaccg ccctgctcct ctagaaaata aggtaaatcg aaatggtaca
     3121 tggtgacaat cggttcgatt ccctgattca ggcattcatc aattacttga tgataaaaag
     3181 aaattccctt ttgattggtt ttattatttt caatatccat aattcgcgtc caagcgatgg
     3241 aaaagcgaaa agcttttaag cctaattctt taaacagctg tatatcttct ttatagtgat
     3301 ggtaaaaatc cgaggcaact tcaaaattag ctaccccttc tgtatgctga tacttgtcaa
     3361 taatagacgg ttgtttccca tcctcctgat aggcaccctc tgcctggtag gcactaatgg
     3421 aggcacccca taaaaacttc tctggaaatg ctgaaagctg tttgtattgc acaaaaatcc
     3481 tctccttaac taatttgcat caatggttcg tgttccttta agcttccgct tgcttcacta
     3541 attcctgcgt aattttcact gttggtcact accacaatca ctgtatcatc gtagccagcg
     3601 tttctaatgg cagcgaaatc ggcgttgatt aataattgtc ctgctgacac ctgatctcct
     3661 gcttttacaa atacttcaaa gggttcccct tttaaattca cggtgtcgat ccccacatgg
     3721 atcaataatt caataccttc tttagaacgt agaccaatcg catgtttaga ttctaaaata
     3781 gtaatgactt ctccctcaac aggtgaataa atgctgtttt cttctggatg aatggctacg
     3841 ccctttccca atagtccctt agaaaagaca gcatcgttca cttcagaaat aggacacacg
     3901 gttcctttta ccggattttc aacaatgact gcttcggcta ttgccgtttg ctctgattca
     3961 tcagaagtat cgtcaaatcc aattagataa gcaatgataa aggttgcaac aaaggcgatt
     4021 acacacgtaa taattgcgtg aacaatattc attggatttt ctccaataaa ggctggtaag
     4081 gctgctaagc caggggaaac aaaggcatat ctgactaaac cgctcaagcc agcatacaaa
     4141 ccagcaattg caccaccaat catggaagca actaatggtt ttttcagctt caaggttact
     4201 ccatagagtg aaggctcagt aattcccatt aaagcagtga tcccagtgga cacagctagc
     4261 tgcttcaact ctttgttttt agttttcgcg gctacagcaa aggtagcacc tgattgagca
     4321 atattagaag ccagcattcc tggcccattg atcatttcag aacccgtact gctcaattgc
     4381 atagtagcga ttggcgtcat tgcccaagct gtacccgtaa ccaccaaaaa tggctgcaaa
     4441 ccacccatca aaaatggaat taaccaagct gctttgccgt taatccattc agcccccatt
     4501 gctataagat cgttcagcaa cgttccaaac ggtccaatga caaccaacgc taaaggcccc
     4561 ataattaaaa ataccagcat cggcttagta aagaatttaa taaacgaagg cgaaattcga
     4621 tccgcaaagg gctctacata ggacatgatc caaacagtaa agataatcgg taaaactgat
     4681 cccgcataat ctgcagctaa taccggaatt cctagaaaag aaacggcatc gccagtagaa
     4741 aaaagctgtg aaatatttgg atgaagcaaa atgccagcaa cagtaatcgc caaaattggg
     4801 ttgacatcaa actttatggc tgccccatag gctaatagta ctggcataaa ataaaaggct
     4861 gcatcagcaa tagtattcaa aatgatgtaa gtttgactac tgtctgatag tacaccagtc
     4921 aaagtcaaga cggccaaaat ggcttttacc atcccggcac ctgttatagc tggaatcatc
     4981 ggtgtaaagg tggtagaaat gacactaatc aatcttgaaa taatactttc ttgattgtct
     5041 gaagaggggc cctttttaat tgaagttccc acttcctttt gaatttcctt gaaggctgtt
     5101 tggacttcat tgccaatcac cacttggtat tgtccaccgc tatccaccac actaattaca
     5161 ccaagtgttt tttctaaggc tttcgtatcg gctttccttt tatctttgag ttcaaaacgc
     5221 agtctggtgg cacaatgggt taagctgacc acattttctt ttcccccaac ctgttgcaat
     5281 acatttcttc ctacttctct gtaatccatt attctctctc cttaaagcag taaaaaagac
     5341 caagtcaaac cacgccccaa tcaacattga agtgttagtt tgtcttggtc ttgcctgcat
     5401 aatcagtaac atgccaataa cgttattttg ctgtaatcca ataatagcac attatgatag
     5461 cgcaatcaac agggaattta attttttatc ttttcgtttg accgtatagg gtaatataac
     5521 agttagatca catcaactag ggtatatctg cgtatactta ctgctaaaat aagtatatct
     5581 aatattaact ataccagtaa aaccattcct acataaccag cgggaaattc ccaaaatcgt
     5641 gaaagcatca aaaatgcata taacggatca cacaacttca acaattctgc tcttgggaaa
     5701 aattgatgaa tttggtgagt aaaggatgga ataaacagca ttccaacaat ataggcaaca
     5761 atagtgatac ttgttttttt ggtaaaaatc agtaagcaat agccaatttc aatcattccg
     5821 agatataaag gtatcgatag aatgatttgg gctagtattc ttaaaaatta actagcaaat
     5881 gtttaaactg atttatttct aatctacatg tagtaagtat ttcaattcca ccaattatca
     5941 ataaaaatac caaacatatc cataataatt ttttcttttt catttctttt atattgttat
     6001 aacagtgaat aacataaaaa ttcccgacaa tcattgtaat atttttttgt cacctgtatt
     6061 agtatttaat ttataggaaa ctataaaaac tccctagtga atttacacct ttgggagttc
     6121 ctgtttggac ggtatgcata atacatttaa atcaatttaa cattaacacc tcagcatatg
     6181 gtatctccac atatatacgg tctttctttg gatagtactc aatatttatg ttgatagcac
     6241 ttccattgtc taatatagct ttagcccggt atgtgttatc tccattttca tgagtaatcg
     6301 tcacatcatc acatgatcga tttaagttgt actgttcttg taaaatctgt gttaccaatg
     6361 gttttgatga cttctctaat ccagatgtac ctgtcatttt tgaaaaaata atacctataa
     6421 tagccaagac aacaatacct ataatgacaa acttcttgtt catatttact ttattcaact
     6481 ttttatctcc tatctccaaa ataatttgct cttatccctc ttatcagtac attgacatcc
     6541 ctaaaagtac accaaagatt gctccagcta cccctgctat catcaaaatc gtacccattt
     6601 tctcatcttt ttctcgatat aagtagccca taataacagc aacagcacca aatacaacag
     6661 gaataaatgc taacgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     6721 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     6781 ttactgtgtc tgttccggtt gtctcatctg tttcttgtag tgctccacaa aactggcaat
     6841 acttactttc atctgttatt tctttgccac attttctaca atacatgtct tctcctccta
     6901 aaatcattca attgcttatt agttaactgt ttactttatt aaatacatca gaaaacagct
     6961 cattcattgg tacacttaat atctcagaaa gcatcttcat ttgatagggt ttaggaatcg
     7021 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt tttgctaact
     7081 ccgtttgaga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     7141 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatataa
     7201 aaaacttcaa ataccattac aaatacaaca acttgttgta ctatactgag atgggggtgt
     7261 attaatgaaa cagaaacaat atgcaaaggt cattagagaa aagcgaaaag ctctcgggtg
     7321 gacacaaaaa gaattagctg aaaaaatctt ctctacacaa caggcagtag caagatggga
     7381 aaattctgta acagagccta acctcgatag cttaacagct ctttctagag ccctaggaac
     7441 ccctgtaagc cattttctag acaatgttgt cgtagattat gaggaagagt ttttggctct
     7501 ctatcgttct cttagcactg aagatgcagt tcgaacaatt gactatatga agctattaaa
     7561 aaggcaagaa aatgaacgca atcagctatt gaaagattga aaaataaata acccaccgaa
     7621 ctttaacggt gggtgtattg agatgaaata agattaagag ctatggataa cctgtctagt
     7681 ttaatacaat tgggtctttt ctcttcctta ctctccgtcc atcatctctt gttacacttg
     7741 taaattgata cagaggaact atcccagctc ctgataatat caattctttt attgtttcat
     7801 ctatccctgc ccctttcgct aaatatatta aaaaactact acttgctttc acattgtata
     7861 gtttcttaat atccttagaa gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc
     7921 tgttttttat taaagcattt ttcacgctgt tatccccaac attacgtagg cgttcacaaa
     7981 cagtgttaat aagttctttc tctgtcatta gatatgtccc ttgccacgat aagccctgta
     8041 aaacttcatg tgcttcatct aaaatcaatt gtttttcctt gctgagttct gatcgggaat
     8101 tttgaggaaa tactctttta gcctcttttt cgttaaaaca tatcttaacc cattcataag
     8161 aaaaatattg atatgtaaaa tgaatttttt ccagaagtgg aaggatatct tcattcaatt
     8221 tttgaatcac agaagttgca ttaaaaacgt ctaatcgata gacattgctt ctcttatgat
     8281 taaagtttcc taacgctttc tcttccttta acctatttag gtattcgagc atcttcaaag
     8341 gaacatcttc atcatctaat ttttttatca atcctataaa agtcataagt gatagtaact
     8401 tattacattt atctatgtca taagttttac catagtaaga actcattttt ttagctaatt
     8461 gttcccccga caaaatacaa gtcacacttt ccttactatc atcaaaaaaa ttaacatcag
     8521 aaagcaagac atcatagaca cgtcgcattt cctctagata attccttatt gtcctgaaaa
     8581 gtttgtcttt cattgattca agagtgtcaa aaagtacttt tgattcctct ttcaattttc
     8641 gaaaatgtgt aggatacaag tctaggttct ctaatagcca attaaatgtt tctacccgac
     8701 tgtctttctt taaaattagc atcacaactc caataaaatt ttcactgata tcacaattac
     8761 tggaatgaca ataaaaaatg gaatgcccag ttttactggt aaaaatacta gcagagggat
     8821 tatgatcgtt gtgaaacaga catcttaagt ttttacttga cttaagtaat ttattcatat
     8881 caatggttaa caatctttca taaatctcat taaaagacat gtcggaggaa tcaaacaggc
     8941 agtctttaaa ccactgtttc atttcttctt tgtcgtcatt ttttatcaac ttaacaaaat
     9001 cggtttcatt gactagttca tactctctcc ctgaatcact ttgacaattt ttagaacatg
     9061 tcatggtaaa atcaatcgga agctcagtaa tatctaacat gttgtcttca ttaattatta
     9121 tcactgcatc tgacttatat cctccaaaaa acattctatt cgaagcattt acattgtcat
     9181 cacacccggg gatgttcttt ttcaagtatt cgtaaacagc aaccatttta ccatagtcag
     9241 taattgcttc ttttaataga aagacaatct tgtatttttc tagattttca gagtaccgaa
     9301 tacttttttg tatcgcaaac gcagaattat gaactaaatt gatagataaa accttttcta
     9361 cgcttaagta tctactatca tctttactca aatctactac cttccccttc tcatctttaa
     9421 cagtgttatc gacatccaga ataattactc tggtttccac aaaactctcc ttgtcattgc
     9481 ctttattctt tgtcttgctt ggaataaatg attgtgatga atttagtatc tctacaaact
     9541 catctcttgt aacttctcta tagttatcta aacacctatc tcttacatca gttttttctt
     9601 tttttgtaac tggcttctct aataattttt cttgatctac ataaattgga atattaatca
     9661 ttgatgtttc catttgtgtt tcctccttat aaaattctgt taaagactaa aacatacaga
     9721 taataaaaac aaactcttta ttactatatg ttttagtctt atttgtttac tcaaccttta
     9781 taccgttcca tgaaactatc aatatcagat tttttaaatc taatgaccgt atcaattttt
     9841 ataacaggta acccgtattg agaaacatat tttctaatcg ttcctactga tacacctaga
     9901 tattcacttg cctgctgata gtccatatac tctagagtga ctccagaaat aatgtcttct
     9961 agcataggct ttagcactgt aattatctct ttcacttgtt gttcaacttg tatagaaaca
    10021 atgctatcaa tgatttgtag agacatattt ccccctcctt tcgtgattgc ttgaacaaat
    10081 tttctaagaa ttatcttcgt attttttgct caaaataaaa gtattttgtt attaaccgat
    10141 taatcaaaca aaaaatgatt accagttcat cgacatacac ataatacctc gataaaaaag
    10201 gggtgtcaac agctttgtaa aatttatttg caacacccct tttttgaggt atactaattt
    10261 taggaggtgt ggtcaatatg ataaaacgtc caccaataaa ttatttagaa cgaaaaaaga
    10321 tactcggaac aaaaataaaa gctataagaa agagtaaaaa attaacacaa ccagcatttg
    10381 gtcttatgat taacaacggt caattaattg acaaaaaaac tatctacgaa tgggagaagg
    10441 gaacctattt accaatacca gagcgattat cccgaattgc tgatttggga aatatgagta
    10501 tagaggagtt agtttgtggc aatgtagaag aatatatttt gggaattatt ctttatagag
    10561 atagcattgt actagatggt ataacatttc cagataaaaa cctgttccag catcttcgtc
    10621 aacagttccc accagtacac tctaatttag atacatggct ggatagatat tccaaattag
    10681 aaccagagat gcaagaattt attgctaaca agacttgtaa taaagttaaa aatgaaaaaa
    10741 ttagtctttt caatatctta aaaatagaag aattattcat caatgctatt gtagaagaat
    10801 ttgacaataa cattcttttc ctcactagca gtatagaaga actactagaa agaatggtag
    10861 atgaatggct acctattcaa ttgaaagaca tgtcttatcc cgaagaagct gtgagagaaa
    10921 ttactgataa tataaacaag ctagaacaaa cgatttcatc aattgggaaa aaatacacta
    10981 agaaaaaaat gaagggaggt gataccatct agtggtaagt actccttgta aactaagagg
    11041 agatgattta atggcaaaaa tatcaaagta caaaaagaaa aacggtcaag tggcgtggat
    11101 gtataaagga cacattgcca ccgatccacg aactggtgaa aaaataaata ctacgagaag
    11161 aggttttagt agcaaacaag aagctcaaaa agattatgac gagtacagac atcaaatatt
    11221 atacggtgtt aaaaagaaag cacctgatat gacatttgaa gacctgtata atgaatggat
    11281 aactcatcag cgtaccagtg ttaaagcttc aacaatcgca atctcagtaa gatacgcaaa
    11341 taatcaaata cttccagcat ttggtaagct taaactatcg aatatctctg tcccatattg
    11401 tcaaaaagtg gtagatgaat ggcacagtaa atatgaaagt tacgattata tgagaaaaca
    11461 gactgcacaa attttacgct acggggttgc aatgcaatat attgacaata atcctatgga
    11521 aaaaacactt cttccacgaa aaaaagagta tgagaaaaat cgtaaatttt actcaaaaga
    11581 ggagcttaat aatttactgg atgcttttaa agattttggt aacatgaaac aatacgcctt
    11641 cttcagatta ttggcataca caggtatgag aaagagtgaa gtactcgctt tacagtggaa
    11701 agacatagac acgtttaata aagaactaca tgtcaataaa actcttgctg tagatgaatt
    11761 tggtaaagtt ataattcaat ctccgaaaac aagagcttct agacgagtta tctctttaga
    11821 tactgagact ctttctatcc tcaacaactg gaaattacag caaaaagaag agtatttaaa
    11881 acttggatac aatactagct ctaaagagca acatgttttt acaacagtta aaaacactct
    11941 atacattcca aacactgtta atgattggtt aagatatatt ttgaaaaaat ataacttgcc
    12001 tcgtattaca cctcatgggt ttagacatac acatgctagt ttattattag aagcaggaga
    12061 atctgtcaag gtagttcaac aacgattagg gcatgaaaat agtaaagtga cattagacat
    12121 ctatgcacat attacaaata atgcacctaa gaaaacaggt caagactttg ctgatatgat
    12181 ggctcatcag taaaagagct ggtcaaaatc tggtcatttc tagtatacag gcaaaacaaa
    12241 aaagctagaa cccttatata tcaagggttc tagctcctat aacaggttta cccaacactg
    12301 ccttccatct cataactgat caatcgattg agttcgactg catattccat tggaagttct
    12361 ttggtgaatg gttcaacaaa gcccatgacg atcatttcag ttgcttcaga ttcggtcagg
    12421 cctcggctca tcagatagta gagttgttct tctgagattt ttgagacttt ggcttcgtgt
    12481 tctaaagaga cttggctgtt gtggatctca ttgaatggga tcgtatcgga tttcgaccat
    12541 tcgtccatga tgatcgtgtc acattcgata tgagagatcg aacccgcact gtttttggca
    12601 aaggttactt gtcctcgata gtttacttct ccgccgtctt ttgaaattga tttagagacg
    12661 atagagctag atgtatttgg tgcattgtgg atcatttttg cacccgtatc ttggatttgg
    12721 tttgctcctg caaaggcgat ggagagcatg gtgcctcgtg caccttttcc atctaagtaa
    12781 acacttgggt atttcatcgt tgtttttgca cctaggtttc cgtcgatcca ttcaacggtt
    12841 gcgccttcgt atgcttttgc tcgtttggtt actaagttgt acacattgtc tgaccagttt
    12901 tggatcgttg tgtaacggca atacgcatct tttcttgtaa agatttctac gatggcggca
    12961 tgcaggctgt tacttgaata agtaggtgcc gtacaacctt cgacatagtg gatgcttgct
    13021 ccttcgtcaa caatgatcag cgtacgttcg aattgccccg tgttttccgc attgatccgg
    13081 aagtacgttt gtaatggaac atcgactttg acgccttttg gcacgtaaat aaaggtccca
    13141 cctgaccata cggctgaatt cagcgctgct aatttgttgt cggttggcgg tacaagtttt
    13201 gaaaagtact ctttgaataa ttcagggtat tctttcaaag cagagtcagt gtcagtaaag
    13261 acgattccta acttttcgaa ttcatctttc atattatggt acacgacttc tgattcatat
    13321 tgtgcggaag caccggctag ataagctcgt tcagcttctg gtataccaat cttttcaaat
    13381 gtatctttga ttttttctgg tacgtcttcc cagtcacgag cgggtttgtc acttggtttt
    13441 tggtaatatt tgatggcatc gaaatcgatg tctgaaaggt cggggcccca tgtttgcatt
    13501 ggcattttgt taaatgtttc tagagatttc agacggaatt caagcatcca ttctggttcg
    13561 cctttgattc ttgacatctc acggatcact tcttctgtca agccttctcc tgtactgaaa
    13621 accggttcga catcatcgtg aaacccgaat ttatattctt ctaattctgg tacgcccatg
    13681 ttctcactcc tcttccttgt ggtaatgatg gaaagctgcg gtaccatcgt tttccattgc
    13741 ttttcctagt gctttccagg caagtgtcgc acatttgatc cgagcgggaa atttagctac
    13801 accaccaagc atagcagcat cgcctaattg gtcgggatct ttgacttctt ttccttggac
    13861 aagctgtaga aagtctacag ttaaatgttc tgcttcttct attgattttc cgatgacggc
    13921 gtctgtcatc atcgaggcac ttgccgtact gatggagcag ccactgccgt caaaggcgat
    13981 atttttgatg attccgtctt ttacatcgac ttgcaattcg atcacatctc cacatgtggg
    14041 gttgttcaat tcgatcttgc ttgtcgagtc agtcaagctt ccacgatgat gcggatggga
    14101 agaatgatcc aagatcactt gacggtataa attgtctaat ttagatagtc ccatgttgga
    14161 aaaactcctt tgttgccatg attgcttcaa ctaaacggtc ggcgtcttgc gttgtgttgt
    14221 agaaataaaa gctcgcgcga gcagtcgctg atacttcaag gtacttcaat agcggttgcg
    14281 cacaatgatg acctgctctg acggctaccc cttccatgtc taaagctgtc gcgacatcat
    14341 ggggatgtaa cccatccaaa ttgaatgcaa cgactcctgt ccgaaaattc gggtcttgtg
    14401 gaccataaac agtcaatcca tcaattgctt gtaattttgg caacacataa gcggctagtt
    14461 cttcttcata tcgatggata ttgtccatac cgagtttttg caaataatcg attgcatggc
    14521 ctaatgcgat agcgccggcg atatttggtg tccctgcttc gaatttccaa ggtagttctt
    14581 tccatgtact gtcgtataat tcgacaaaat cgatcatttc gccaccaaat tcaacaggtt
    14641 ccatttcttc taaccatttt cgtttaccat ataaaacacc aatacctgtt ggtccgcaca
    14701 ttttgtgtcc gctgaaagca aagaaatcac agtctagtgc ttggacatca atggccatgt
    14761 gcggggcaga ttgagcacca tctactacga tgaccccgcc gttttcatgg acaagttcag
    14821 ctaattcttg aacaggattg aagacaccta agacattgga tacatgagca atggagacga
    14881 ttttggtttt gtccgtaatc tttttcgctg catcctccat atccaggaag ccttcttctg
    14941 ttagttcgat atatttcaag acagcacctg ttcgttttgc caactgctgc cacgggataa
    15001 cattagaatg atgttccatg taagagagca ctatctcgtc tcctgctttg atatagcggt
    15061 cgccgaaggt tctggctacc caattcaagc ttgtcgtcgt accacgagta aacagcactt
    15121 ctgctgtttc ttttgcatgg atgaacgccc gtactttttc tcttgcttgt tcataatcat
    15181 gggtcgcgcg ttctgctaac gtatggacac cgcgatgaac attggcgtta tcgtgttcgt
    15241 aatagtacgc taatgcctgg agtacttgtt ttggcttttg ggtcgtcgct gcattatcca
    15301 gataaactaa aggttcatca ttgacgtctt gaaataagat aggaaaatca ttgcgtagct
    15361 ttttagcatc gatcatccat tcaacttccc ttcgattact tcgactaatt ctttccgaac
    15421 ttgttctaca gggattgctg ttaagacaga accgaggaat cctcgaatca ctaagcgctc
    15481 tgcttcttct ttatgcaatc ctctactcat cagatagtac atttcttctg gatcaactcg
    15541 tcctacactg gcagcatgtc cagcagttac ttcgttttca tcgatcaaaa gaataggatt
    15601 cgcatcacca cgtgctttat cagaaagcat caagacacga ctttcttgtt gggcatctgc
    15661 accttttgcc cctttcaaga tgtggccgat accattaaag gtcaatgttc ctttttctcg
    15721 gatcactccg tgttgcaaaa tatgcccgat cgtatgaggg gctttattgg ttactcgtgt
    15781 atcgattcct tggatttgtc tgcccgagct gattgccacg attttgactt ctgcgtgtgc
    15841 accttctcct gctaaatctg agtcaaagtc agcgatcaca tgaccgtcat tcatgacacc
    15901 aattgcccag tcgacagaag catctcgtaa aatgtgccca cggcgattca tataagaagt
    15961 aatattttct cctaattgat ctacggctga atatttgatt tttgaaccag ctttagcgat
    16021 gacttccacg atgatattac ctgatgattt tgctacttgt tctttggttg tctggaaacg
    16081 ttccagataa gaaaattcac tatgttcatc tgccacgatc aacacatgct tgaagaagtg
    16141 ctcatcggat gcgccatctt gaatgaataa ggactcgagt ggttcatcga tcacgacatt
    16201 tttaggcaca tacaggaaaa taccgctgtt cataaaagct acatggagtg cagtcaattg
    16261 gtcttcgttc atgtttactg cttttttcat ataatattct tggactagtt ctggatattc
    16321 gatcattgca gtgaacaagt cggtaaaaat gactcctttt tctgctaatt ccacaggcag
    16381 ttgttcaaaa atcgtccatg aaccttgttg gacaagcaca ggattgtctt tcattgcatc
    16441 aaatgccgga atcgtgcctg tttcactgat ggtttgtgtt tctttcacat caaataacga
    16501 ccagcgatgg aatttcaccc gatcgatatg cggcaatggc agttcatctg ctttcgctag
    16561 tgcgtcttga cggaaggttc tcatccaatc aggttcgcct ttacttaaag aaaaatcatt
    16621 taccgcgtca agataatcga gccagttttt ttctttcatt cttgctcccc cttacgcttc
    16681 ttcttcttta tagtcgattc ctaattcttt actgatacca gcgtagcctt ctgcttccag
    16741 acgtttagct aggtcagcat tccctgtcat gactactcgg ccttccatca tgatgtgaac
    16801 gacatcaggt gtgatatagt tcaataatcg ttgatagtgg gtgatgatca atgcaccgaa
    16861 attgtctccg cgcatttcat ttactccttt agcaacgact ttcaaagcat cgatatccag
    16921 acctgaatcg atttcgtcta aaattgcaaa tgtaggttct aacatcaaca attgcaggat
    16981 ctcattccgt ttcttttctc cgccagaaaa accttcgttc agataacgtt cagccatttc
    17041 ttctggcata tttagtaaag ccattttttc atctaacttt ttcaaaaatg ccataacaga
    17101 aattttgtcg ttttcatcac gtttcgcatt gatcgctgca cgcatgaatt ccgcattcgt
    17161 gatacctggt atctcacttg gatattgcat agctaaaaat aagccaagtc gcgcacgttc
    17221 atctacttcc aagtctaaaa catttttccc gtcaaacaaa atttcgcctt ctgttacttc
    17281 atagtttgga ttgcccatga tagcggcaga taaggtagat tttcctgtgc cgttaggtcc
    17341 catgatggca tggatttctc cggttttcat tgttaggttt acgcctttaa ggatttcttt
    17401 ttcctcgatc gacacatgta gattttttat ttctaagaca gacatgcatt atacccccaa
    17461 attctctttc gttctcactc attttagact aattgagaat gaagcgcaat gaccttcctt
    17521 actaaaatca aatatctttt atttattatt aaacaatttt gatttctaaa ttagaatagg
    17581 tataaaaaag atgagaaata agccattaca gacttatttc tcatcttttt tgttccatac
    17641 cttattttgc atcagccttt actggtttaa cagagccgcc ccatttgtct gtgatccatt
    17701 cttggacttc tttgcggtga agcacttcaa tcagtttttt gattttgtca ctgtcttcat
    17761 cacctttacg agtcgcaata atattcacgt aaggagagtt gtccttttca agcaatacgg
    17821 catcttctaa tggattcaac cctgcgccgt aagcgaagtt tgcgttgatt gcaactaggt
    17881 caccttcatc attttggtac gtagaagcta aaagtgatgg gtcgatcgta tggttgaact
    17941 tcaagttctt agggttttct tcaatatcat cgaatgttgc tgtttccaga tctactccat
    18001 ctttgacttt gactaagcca gcatcttgta gaattgtgat gatgcgtccc cagtctgatt
    18061 cagaatttga agtaatgatt gttgcaccat cttttaattc ggaaacatct ttgacttttt
    18121 tagagtacaa gcccattggt tcgatgtgga cagcaccggc attgacaaag tcatatcctt
    18181 tttctttgat ttctttgttc aaataaggaa tatgctggaa gtaattggca tcaatgtcgc
    18241 cttcttccaa agattggttc ggtaatacat agtcatcgaa tttgacgatt tcaagatcga
    18301 ttccttcatc tttcaataat ggtttcacat gttccaaaat ctctgcgtgc ggtgtcggtg
    18361 aagcgccgac tttcaatgtc gtgtcgtctg ctttatttgc tgaatctttg ctgtcgctgt
    18421 tgttaccgca tgctgctaat cctacagtca gcaaaagcgc tgctgcaaat ccaaaaattt
    18481 tctttttcat atcctacatc cccctatatc ctattatctt ttgtctgttt tcttagttaa
    18541 gaaatcaccg attccttgaa cgataaatac gattagtaaa ataattaaag tcgcaacgaa
    18601 tgtcactgtt agattaccac gctggtagcc ttcctgccaa gcaagacctc caagtccacc
    18661 tgcgccgatc gcgccggcca tcgcagtaaa tccgatcatc gagatcgttg taactgttaa
    18721 tccagaaatc aatgcgggaa tactttcagg tatcaagacc ttccagatga tttcccagta
    18781 acttgctccc attgcatctg ctgcttccag tacaccaggg ctgacttcac gaaaagcaat
    18841 ctctactaga cgggcataaa atggtgctgc ggataaaaca agagctggaa tggctgcttg
    18901 cgcacctagc atagtgccta cgattgcttt agtaaatgga atgatcaaaa ccatcaggat
    18961 cataaatggc gttgaccgga aaatattact gatgatcgat acgattccat aaatgattcg
    19021 agcatacggt ttgtttttgc gtccaataga gtaaaggatc aatcccaaaa ctaatccgat
    19081 gatcgtcact aagaccattg agattaatgt catatatagt gtatcaagga tagcctgatt
    19141 catcgtttcc gggttgattt gtttaaaatc tagatatgtt tctgaaaagc ttttatccac
    19201 gatggatcac ctcaattcct actccttgtt ctttgatgaa cacttctgct tgatctaatt
    19261 ctttggtttc accagtcatc aagacagtca atgaaccaaa ggaaccttct ttcacttgtt
    19321 tgatctttcc atacacaacg ttgacatcga tgttatagcg tcgaatcgct tgggaaatga
    19381 ctggctcgtt ggcatttctt tcgctaaagg tcaaagttgc caaacggccg cttggatttt
    19441 ctttgatgag ttcagctaat agttctgtcg aatcagcttc tggttcgatg tcttgttgta
    19501 caaagcgttt ggttacttct tttttcggtt gtctgaagac agtcaatacg tctccttctt
    19561 ctacgacttt ccccaactcc atcacagcga ctttgttgca gattttgcgg atcacattca
    19621 tttcatgtgt gatcaagacg atggtcaaat tcaatttctt attgatcgct aacaataagt
    19681 ctaatacttc ttctgttgtt tgcgggtcca atgcgctagt agcctcatca caaagcagga
    19741 tctcaggatc atttgccaat gctcgtgcga taccgacgcg ttgtttctgt ccgccggaaa
    19801 gttgtgaagg atatgcttgt ccgcgtcctt ctaacccgac taaacgcaac agttcttctg
    19861 cccgttcttt acgtttgctc tttggtacgc cagctaattc cagcggcagc tggatatttt
    19921 caagtacagt tcttgaccat aacaggttga aatgctggaa gatcataccg attttctttc
    19981 ggaatgtacg taattctttg ttttttaatt ttgtaatatc ttgatcgttt acgatcactt
    20041 cgccgctcgt gggaagctca agtccattca ggagacggac taatgtactt tttcctgcgc
    20101 cagaatagcc gacgattcca taaatatccc ctttttcaac gtttagtgaa acgtcattga
    20161 cggcttggat gtttccgttt ttcccattga atgtcttttt gacattcgtt agttggatta
    20221 atgacatgcc attcacatct ttctcttatg atttttcaaa caaaaaagcc ttcgccctct
    20281 tgccttaagc aaaaggacga aagcttgaac ttccgtgtta ccaccttgat ttaccgttac
    20341 ttcgcaataa cagcctcttc cagtacttgg acttagcttc tttccgtata ctgtcgcgct
    20401 gtaacgggca ctcccgtagt aagtttgctt acgctcgctt actctgctct aagaccatct
    20461 tccatctcat tttcgttacc cattttcagc aaacagggct ctcttcaaac gtccgtgaca
    20521 tgtactcttc ttttcaaagc tttctcgttt catataggat tgcttatgat tatagcaact
    20581 ggaagaatca gtgtcaactt ttttcaagtg attttctgat tttataattc gtcaaattct
    20641 tttggatcaa catctttgaa acttaagatc cacgcattca tttcatctga atcattcaag
    20701 atattgatat cttcaccgat tttatcatta acagaagaaa ctgtacccgt tagcgggctg
    20761 gcaaattcat tgaccgcttt ttccgcttca acttcgatca gaggttcatc tttttttagc
    20821 gattgtcctg cttttggcag attggcaaac gtgatgtctc caagttcttc ttgcgcttca
    20881 tttgtcaaac caacacagta ttcttttcca ttaaataaga tccaaaggtt gtcctttttc
    20941 ttcaaatgct tgcctgccat tttatatcag ttctccttca taaatttttt cattgaatcc
    21001 aattgcaaca agctggtcct ctcgtaagat cagtggtcgc ttgatcagca taccatctgt
    21061 cgataagact tggctcgctt ttgaagccgt aaactcgcct acttgatctt tcagtccaag
    21121 ttcccgatat ttcatcccgc ttgtgttgaa caggcgacgg attggtactt gactcgtttc
    21181 tagccaagtc tcaatcgttg aggccttcgg tacttctttg atcatatcaa cggtttgata
    21241 actaacgcca tgctggtcta accacgcttt ggcttttttg catgttgaac atttcgggta
    21301 ccagtaaaag gtgtacaatt ttcttacctc cttgaagatc ttgcaggtgc ttcattttgg
    21361 tatctcatcg ataatatcaa cccgataccg atcatgttcc ctaaaatcga ggatcctccc
    21421 tgactaatga aaggcaatgg gatccctgtt aaaggaagta atccgatatt tgctccaata
    21481 ttctcaaaga catggaacag cagcatcatg atcaaaccgc ttgcgatata ggcgtaaaat
    21541 tcattattcg tatcgaaaca cacccgaatc atacgataga ttaaaataaa atacaagaaa
    21601 atgacgaatg caccaccgat aaagccgaag ttttcaccga taacggtaaa aatcatatcg
    21661 gactcacgaa caggcacata gatattgctt acgttgaatc ctttaccaaa caagccaccg
    21721 gtaccaattg ccaaaagtcc ttgggccggc tgataactca ttcctgacgt atcatggaaa
    21781 gggtccagcc atgaatcaat acgtgcaaac tgataagatt tgaatccaac tttataaagc
    21841 aagtctcttc cgccttcagt cgtaaccaag aagatcgttc ctgctccaac gactactgcc
    21901 aagattacta caggtgcgat gatgcgccaa gagattccag acatcaggaa aatcccgcca
    21961 aagatcgcaa gaaagacaag catcgttcca aagtcatctt gggccatgat caatgctaaa
    22021 accggtattg tcacgatcaa gagcttccca atcagccaaa agtccgattt catcgtccgt
    22081 tcttttactt gcgtattatg tttagttacg atcagtgcca tcatcaagat gtacgcaatc
    22141 ttcatcaact ctgctggctg gaaagtaaac gcaccgaatc gaaaccagtt tttagaccct
    22201 gttgaagcag ctaaattctg atcatagaat ttgagcaaga gtcccattac cactaacccc
    22261 aaaccataaa ggtaaggagt cagtttccag atccatttgg aattcaaatg catgatgaca
    22321 acgattgctg ccgctccaac taaataccag acaacttgca tccctacgcc tttcaagact
    22381 gctggattgt tcggatcatg gctaagtgct acatataatg agagcagacc aatcaaacat
    22441 aacagaaaga ctggtaaaat cacgccataa tcaatacgat tatcgttcga agcttttaat
    22501 tttttttcca tgtttcatcc cttttcttaa aaaccatact gctgattatt atagcggtcc
    22561 agttagataa tggaaagtct ttccatagac atttgggaaa aattaacttt ccgtttgtgc
    22621 agttgcttga gcaaatgcag tacaaaagcc tactgcttat ctttagtgta tcatagaact
    22681 ttttttgata catgcaatta tctaccagct gagattctcc tcgctgtgag aagtttctgt
    22741 gctttcattg tatcgaagga tctcttcggc aatctctagc agctgatcag aaaccagatt
    22801 ggttcgttgg atgaaataaa aatatcgatg gaaattcaac ggagtcattg gcacattttc
    22861 tggaacagag cgcttagaca aaatcgagcg accgattcct tccttcagca acgcaacaat
    22921 tacatcgtta cttttcacga tcatcttctt atcttggata ttcatctctt caaaataacg
    22981 ctgcgtgtaa tgaaaaacac cagagtttgc ttctcttaca agccacaatt tgctttttgg
    23041 gtccccagcg atgactagct ggtcttccat caaagagtat ctttgtacgc cggaagtctc
    23101 taatggcttt tcgataaaac caaaatcgga ttcatgtttc gctacttgtt ctaatgattc
    23161 atgagaattc gtgatttcga tctcaaaatg cactttagga aaccttttga tcagatgttt
    23221 catcaactga ggcaaaagat agacagcaaa ggtgtgggaa gcaacgatcc gacatacttc
    23281 ttcttcattt tgttcattga gcaatttttg ctgagtttct tcccattctt ctaacaaaac
    23341 cagtgtccgt tcatataata agtcggcttg cttagttgga agcatctctt ttcttccgtt
    23401 acgttgaaaa agatcgatat tcaattcccg ctccaactgt ttgatttgat tcgagactgc
    23461 tggttgagat aaaaacagta cttctgctgc tctggaaaaa tttcttgttt catatactgc
    23521 tcgaaaagtc gttaataact taaacatggc accacttcca taacttttta tgataattac
    23581 tataaaaact atttgattta aatatacaaa aaaccatcta taatagaaat gtacaacaaa
    23641 acagttgctg atccaactag taacttagat gaagacaatc agctgtctgc ttgctcgtaa
    23701 tcatacattc ctattcattt tacaatgtat tcgttcatct tggtactaaa atctattaaa
    23761 taaaaaggag aatattatat atgaagaact ttttgaaaat tattccgatc ccgatctgcg
    23821 ggttgatcct tgggttagct tcccttggaa atctgtttaa agactatcac catgttgctt
    23881 taggcaatct aactggcggt atctctatga ttcttatgat attcattttt gttaaattgt
    23941 ttatcttatt cgaacacacg aaacaaacgt tgcatgatcc aatcatcgcc tccgtttcac
    24001 caacttttac gatgtcactc atggttattt gtacttattt tgtttcatat gaaagtattg
    24061 caccaattgt caaattcatt tggctgtgcg ctgttatctt ccaagtgatc cttgtctttt
    24121 atttcaatta tcaccatgtt atcaaagctg atctttcaat tgaagcaatt tatccaagct
    24181 ggtttatcat ttacgttgga tttggggtca tcactgtaac tgccgggaac ttcttcccac
    24241 ttattggaaa aattttcttt tggatttcat tgatctgcta tgcgttctta ttgccgatca
    24301 tcatcaaacg tgtctttttc gtaagaaata tggctcatcc gacacgtccg ctgattacga
    24361 tcgttgcagc acctggttct ctttgcctaa caggttatct taaaaacttt gctgaaccta
    24421 atttttatct tgtcgtagca ttgttcgctt tatctcaagt attgtatttc gttgtgctgt
    24481 ctatgcttcc acgtttgttc aagcttcgtt tttatccaag ctatgcagcc tttacattcc
    24541 cgcttgttat ctctgcaaca gctatattta ctactgttca ttacttcaac agtttaggta
    24601 tctatagtag cgtattggat ttcttaaaag ttgcggaatg tctgtttgca agtgtagttg
    24661 tcttctacgt tcttgctcat tacgtacgtt tcttaattaa agaacatagc aaacatggaa
    24721 aatttgtttc taaaaaaatc gcttaataat cgattcaata ggttgtgaaa aagctgttct
    24781 gcatcaagta ataagaacgg ccaaacaaaa acagctgttc atgtttttgt actggttggg
    24841 cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggtcgta aaagaagtgg
    24901 tcagcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt
    24961 cggcttattt ccgtagaagc tacttctgga acaccgttta ttcgaaacaa gaggtcgtac
    25021 aagacttttg tcacgacctc ttgttctttt gtcaaaatac tggtttagtg ctactatgtt
    25081 cgagtatgct tttagtattt attgacgaaa ggaaccgatc aaatgaaatc attgtcacat
    25141 ccaataaaat ctgtaaagga ttcagcggtc cgcgaaatct tacccggttt actgctttct
    25201 tttgccatcg ctgccttcag caggcttctt gctatttatc tgccacagtt aggtggagcg
    25261 actattgcta ttttattggg tattttatta ggaaatactt tttttaaaca gcctgttcta
    25321 gcaatcggca caaaatttgc tgaaagcaaa ctattagaat tctctgtttt tttactaggt
    25381 gcaactgtca cagctcaaac gattgctcaa attggtgtaa aaggaattgt ttttgtgtta
    25441 ctgcaaatgt cagtcactat catcggtgct tacttgattg gtaaaaaact gcaattcagc
    25501 gatacttctt ctttgatgat ggctggagga aacgctgttt gtggttcttc tgctattgct
    25561 tcaatcgccc cttccatcca tgcgaaagag gaagaaaaag gacagatcat cacactagtc
    25621 aatctactgg gtactgtgat gatgttgact ctcccttttc ttggattaca gttgtttggt
    25681 ggaaatctca ttgaaaaaag tgcacttttg ggtggtacac tccaatctgt tggtcaagta
    25741 gtcgctgggg caagcttact tgacaattct gttgtcgaat tcgccatgct atttaagatt
    25801 ctacgtatta tgatgctcgt tgttgttgtc tatctgtttg aaaaattcat gcacaagaaa
    25861 caaccagctc tgtttgaaac gccaactgat acgcagaaaa aacgaaaatt accgcttcct
    25921 tggtatgtat tagggtttgt gatcttttgt ttggtcaaca gcttgttttc tttacctgta
    25981 gttattgacc aatcggcaca ttttatcagc agctggtttg agatcactgc tttagcggca
    26041 attggtctgc gtttagactt tgcaaaattc atgaaagaag gtacacgctt cttatcttat
    26101 ggattagctg tcgggcttct ccaaacagtg atggctttac tatttatttg gctgatcaaa
    26161 gtttagaatg aacatgaaaa aacagttgtg attctcgcat ggaacaatgt ccgaatcaca
    26221 actgttttta attttgcaga tatccttcta acaacaacaa ttggcgtttg atttcaaccc
    26281 catctattgc gtttcccata tatcctgtca gttttccatt ttgcccgatc actcgatgac
    26341 aaggaataac gattggcaat ggattggttc gatttgcctg cccaatcgct cgcacagctt
    26401 tcggactatc aacagcaacg gctacttctt tatacgttcg gatttctcca taaggaatgg
    26461 tcgacaaagc tttccagaca ttttgctgaa aaggcgtacc tctctttata gataaaggaa
    26521 catcaaattc ttttcttttt cccgcaaaat attcttcaaa ctgttcttgt gtgcgcgtta
    26581 aaattcgctg attcgctgat tcgctttctg aaattggctg aaaagataca gcagtcaatc
    26641 cggattcatc tgcatctaac cataaaaagc cgataggtgt gtgtattttc attttgtttg
    26701 ctcctagtca atagactgga acttcctcct ttttctctat ccaaaactac ttgccaacta
    26761 ataaaaaatg ttcttaaaag tttgataaac cgaggaaaca tggtaaacta aatacgttag
    26821 tttacaaaaa gaatacctgt tcttttacgt aaaatcaaa
//
LOCUS       DANJLF010000032        26314 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000032 DANJLF010000000
VERSION     DANJLF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..304
                     /locus_tag="PWT25_001516"
     CDS             <1..304
                     /locus_tag="PWT25_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1021608.1"
                     /translation="RFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIE
                     GFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            777..1331
                     /locus_tag="PWT25_001517"
     CDS             777..1331
                     /locus_tag="PWT25_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1021609.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1504..1977)
                     /locus_tag="PWT25_001518"
     CDS             complement(1504..1977)
                     /locus_tag="PWT25_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1021610.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1989..2525
                     /locus_tag="PWT25_001519"
     CDS             1989..2525
                     /locus_tag="PWT25_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1021611.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2565..2936
                     /locus_tag="PWT25_001520"
     CDS             2565..2936
                     /locus_tag="PWT25_001520"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1021612.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            3220..4491
                     /locus_tag="PWT25_001521"
     CDS             3220..4491
                     /locus_tag="PWT25_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1021613.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4736..5761
                     /locus_tag="PWT25_001522"
     CDS             4736..5761
                     /locus_tag="PWT25_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1021614.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            6055..7164
                     /locus_tag="PWT25_001523"
     CDS             6055..7164
                     /locus_tag="PWT25_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL1021615.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            7247..8143
                     /locus_tag="PWT25_001524"
                     /pseudo
     CDS             7247..8143
                     /locus_tag="PWT25_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            8159..8983
                     /locus_tag="PWT25_001525"
     CDS             8159..8983
                     /locus_tag="PWT25_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1021616.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9015..10325
                     /locus_tag="PWT25_001526"
     CDS             9015..10325
                     /locus_tag="PWT25_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDL1021617.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10473..11732
                     /locus_tag="PWT25_001527"
     CDS             10473..11732
                     /locus_tag="PWT25_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1021618.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11742..12614
                     /locus_tag="PWT25_001528"
     CDS             11742..12614
                     /locus_tag="PWT25_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1021619.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12626..13456
                     /locus_tag="PWT25_001529"
     CDS             12626..13456
                     /locus_tag="PWT25_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1021620.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13496..15679
                     /locus_tag="PWT25_001530"
     CDS             13496..15679
                     /locus_tag="PWT25_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL1021621.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15740..16960
                     /locus_tag="PWT25_001531"
     CDS             15740..16960
                     /locus_tag="PWT25_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL1021622.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16957..18417
                     /gene="gtfA"
                     /locus_tag="PWT25_001532"
     CDS             16957..18417
                     /gene="gtfA"
                     /locus_tag="PWT25_001532"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL1021623.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18494..19600
                     /locus_tag="PWT25_001533"
                     /pseudo
     CDS             18494..19600
                     /locus_tag="PWT25_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19922..20131
                     /locus_tag="PWT25_001534"
     CDS             19922..20131
                     /locus_tag="PWT25_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL1021624.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20124..20273
                     /locus_tag="PWT25_001535"
     CDS             20124..20273
                     /locus_tag="PWT25_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL1021625.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20427..21092
                     /locus_tag="PWT25_001536"
     CDS             20427..21092
                     /locus_tag="PWT25_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1021626.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21376..22461)
                     /gene="xerS"
                     /locus_tag="PWT25_001537"
     CDS             complement(21376..22461)
                     /gene="xerS"
                     /locus_tag="PWT25_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL1021627.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22795..23028
                     /locus_tag="PWT25_001538"
     CDS             22795..23028
                     /locus_tag="PWT25_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL1021628.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23025..23432
                     /locus_tag="PWT25_001539"
     CDS             23025..23432
                     /locus_tag="PWT25_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL1021629.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24569..25087)
                     /locus_tag="PWT25_001540"
     CDS             complement(24569..25087)
                     /locus_tag="PWT25_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL1021630.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26205..>26314
                     /locus_tag="PWT25_001541"
     CDS             26205..>26314
                     /locus_tag="PWT25_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1021631.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         8994 a         4012 c         4879 g         8429 t
ORIGIN      
        1 aaggtttatg atagataaat ggaaaaagca gtatccacga gtggttaaat tgctcatgaa
       61 tcctgctata ttaacctttt ataatttccc tccgtcaatc agaagaacta tttattcaac
      121 taatttgatt gaaggtttta acaaacaatt aaagaaatat accaaaagaa aagagcaatt
      181 tcctaatgaa gaatctctgg agagatttct agtatctcaa ttcaacaact ataatcaaaa
      241 gtttctatgc cgagttcata aaggttttaa agaaatacat gatacattag aatcaatgtt
      301 ttaacttgat caacagaatg ggcttttcca tttacacaaa attattgacg ctcccgacaa
      361 aattatgatc ttgcgtaaag ttataaaata ttaataaaaa tattttatta atgaaaagct
      421 tggtagcacg gttattcata tgctaattta tttcacttta tagtaagggg agttcttagt
      481 atattgttca aacttaaatt tcatataatt taattatgta aattagctat tctaagaact
      541 tctacggata actgcccgtc taaaatttaa ataagagctt agaatcttca tataagtaag
      601 gttaattttt ttgtacaaaa aagtcggaat attctgagtt actgaaatga tataaaatca
      661 taaactaaat cccttgattt tattgacttt caaaagttta tatatataag ttaatgatta
      721 tggtaaaata tatacataat cattaactca gaatattttt aagaagggaa acaatagtga
      781 gctataatgt acaaccgtta aggacaaatg aagagataca agattttcag ttttggctaa
      841 gaagaacaag taatccggaa agagatagtt ttttattttt gtttggaatc aataatgggt
      901 tacgtatgtc agatattata ggattaaaag ttggagatat acgaggcaaa agtaagccta
      961 ttattgttga acataaaaca ggtaagcgaa aaccgatttt tatagataat ttgagagaag
     1021 aaattttatt atatactgaa ggtaaagaag aaaatgattg gttatttccg agtcggcaac
     1081 aaggtcgaca tataacaagg gatcgtgtct atcagatata tgcagatata gcagaaaaat
     1141 taggacgaga tgatatagga acacatacat taagaaaaac ttttgggtat cactactaca
     1201 agaaaactag agacattgct accttaatgt ttatttttaa tcatagctcg caagcaatta
     1261 caaaaaggta cattggtata actgaagatg aaataggagc atcacttcgt ggatttaaac
     1321 taggtgtata atttaaaaat ttttataaac agtaaagaca aagattaaaa gctattttta
     1381 tctagaacga ttttatttca taaagttcgc ggaataaaag ctggggttac tggtacttgg
     1441 tataggttcg tgttatataa actaagtaat taaataacat ttttaggttg gaaaaggatg
     1501 aaattaaaaa aagagaatca atacggtctc aaaaacgcaa acgctgcgga ataagaaaaa
     1561 tactgataac tcaacgttta gcagactatc taatagggat agtctgcttt tttcgttttt
     1621 gaccatactt ttgaccttct gcaaacttcc ttgcttttcc tctgtagaac tcatgaaatc
     1681 tgcaaaacga attgccgttt cctctgtctg ctttttcgcg atatgggtgt atatatcatc
     1741 catagtggtt ttaatatctg tatgtccaag tcgaacatac acttctttga cagacgcacc
     1801 agattcaaag agcagactac aatgggtatg acgaaaacca tgaagtgtga tatgttttaa
     1861 gttattctct ttaatgatga ttttcaaatt gtgatttaca tagtctaaat aaaggggctt
     1921 attttcttct gatgaaaaca aaagttgttc accatctttt ataggtttga ttctcatttt
     1981 tagatattat gcccgcacgt attcatcaca tttctattgt gaatcgcttt attcgaccga
     2041 catttgactt ttatcataat atactcggtt taaaattact aatgaaaaca attaatcaag
     2101 acgatcatac catgtatcac ctgttttttt cagataatca ccaccgtgtg ggaactgaac
     2161 tgactttttt tgaagtacaa gaagggaaca accagttttt tggaaccaat acgattgaac
     2221 gaacgatact aaaagttcct tccgaagcat cattgcattt ttgggaaata tattttgaaa
     2281 cacaaggcgt ttgccattat ggaatcgaat cattttctgg acgacctatc ttacgttttg
     2341 aagggccaga tgcaacacga ttagctttag ttcccttacg cgaatttgaa gatattgatg
     2401 attatttccc gtatgtcaca gaccagatcc ctacagagca tgccatttta ggaattgatg
     2461 ccattcaact acgcgtacaa tattccgatg cgactgaacg agagttattg tcggtaggct
     2521 ggtagcacaa agaatacgtc agtttctttt cgggtcatca acgagtgaca atcttagaaa
     2581 atcaagacac tcagttctat caagaagttc agattattca agatcgtgag aatccagttg
     2641 ccattgaagg gattggtagc gtccatcatg ttgcgtttgg cgtagaaaat aaatctgatt
     2701 tacaacgcat cgacaaacaa ttacaagagc gcaattttat aaattcagga ataaaagatc
     2761 gtgaattttt tgtatcactt tattatcgtg acccaaatca attattgatt gaaattgcta
     2821 caggggaagg aaacctagat gcaaaagcgt atgaaaatca aagtccacgc tttgaagaaa
     2881 ttcctttatt tttaccgcag tacttaaatt tcattcgtga acaagtggag caataattag
     2941 ctaatcaaat ccatgacgaa gagtaagagg ttgactcata agtcaaaaaa taaagaaaaa
     3001 cgcatcaaat ccattctaaa gaatgttgat ttgatgcggt tcctattttt ataaactatc
     3061 attttcgact aataaaacta cttatgagtc agcctctctt tttttcttga tttttatacc
     3121 tctttctagg taaactaaac atctgccaaa aataccgtgt ctcattgaaa cattctcaac
     3181 tatttcaaaa taaaggggag taccaaatga acgagtatat tgaaattatc cacgcaagag
     3241 aaacaactta aaaatgtcag tctcaaaatt cccaaaaata aaattagtat ttttaccagc
     3301 gtatctggtt caggaaaatc atcgattgtt tttgatacta ttacccaaga agctggtcgt
     3361 caactgaatg acacctatga tagttttacc cgtttatttt tacctaaata cagtcgtcca
     3421 gaggttgatg agattgataa tctttctacg gcgattgtgg tcaatcaaaa gaagattggt
     3481 ggcagtgcgc gctcaacgtt agggacgatt actaacatca atgcgttatt tcgtgtgttg
     3541 ttttcatgtt ttgcaacacc tagtttagga tatgcaaatg cttattcatt taatgatcct
     3601 agtggaatgt gttctgtctg tcagggaatc ggtaagacga ttgcattttt aataggattg
     3661 ctttattttt cggctattga taatgctgat tttgtatttt ctgatccatt taccgcagaa
     3721 gttttgtctg gaattgagga acaggcaaag aatcatggtt attttgtatt ggtgcataat
     3781 gttaattcag cagaagatgt taaaactatt cagagaaatt ggcgttttga ggggttcatt
     3841 gttgttggtg tacgtgtaaa agaatttttg gaattaaata acgtttttga tggtcctgtt
     3901 gtttatattg atacgcatct ttcaaaagag acattaagag aacttgaaca acaaatgaat
     3961 cgtttatttg taaatacaaa tgattaccaa gctagtacat tggcagtgga acatttgatt
     4021 cattatggtc atgagaaaat agcgtttctt tcttttgact acattgtagg tgaaccaagt
     4081 gttattcaag aacgacatca agcttatatc gatacattaa agaaatatca tttaaccttt
     4141 aaatcgcatt ggatatataa caactctgaa tttgagcgaa tttatcaaga attagctgaa
     4201 tttactgcaa ttgttgttac tggagattat ttagcggcta aattagtaaa atttttaaaa
     4261 aataagaata aacgagtgat ggaacaatta tcgattattg gatttgatga tattactttt
     4321 gcagaattaa tggatcctgc gttgacaaca atacgactaa atccaggtaa caagggggcg
     4381 attgcttttc accaactgat tggactggcc aaacaagaag aaataaaaaa tcaagtacta
     4441 ctattagagg gagaattgat ttctcgagac tctgttcaca aaatcaaata aaaaagctct
     4501 gtgaaataag tatagttaac aataaaatat ccgaacgata gagagaatga actgtgatct
     4561 agcagttcct cgtcgcaaat tcactagaat tagcattatt tactctaaat tgttcggata
     4621 ttttttcttg tttttaatta tgattacagt gcctattttt aacgaaattt gcttattttc
     4681 aaaatgtatg attatatgtt agtaaacact ttcaaatatg aaaagaggag ctattatggt
     4741 aggaattagg gatgttgcaa aacgggcggg tgtgtccatt gcaacagtat cgcgcgtgct
     4801 aaatgaggat ccgacacttt cagtaaccga tgaaaccaaa cgaaagattg cagaatcggt
     4861 taatttttat cattatgtca aaaaaagtcc cgttgcaaaa aatttaacaa atattgcatt
     4921 aataacgaca gtttctgaag tagatgagtt ggaagatccc tattttcgaa cgattcgcag
     4981 agggattcaa gtcgaagcag aacaaagcaa agtcaatttg aagaaaatta ttcgtttgtc
     5041 agaaacaccg ttggagcaag acgatttgct aaaatgtcaa ggtgtgttga ttattgggca
     5101 ggtgctatct tctgtgattg aagaagtatc agcaattaat tcgaatgtcg tagtgattga
     5161 tgaccctagt attgaagctg aagtagatgc agtttatact gatttaacaa aagcaacgaa
     5221 tcagcattta gatcgattgt ataaaaaagg acaccgaaac attgcgttta tcggaggagg
     5281 acgtgttcgt ttagatagcc aaggaaataa acacgtgtca gatgatgatt atcgaaaaat
     5341 agcctatgag acgtggatga aacaaaaagg attagaaaaa tatagtcaaa tttatttaga
     5401 aggctggacc acacttgacg gaatgcaggc agcagattta cttttgaaaa aacatgctaa
     5461 agataagttg ccgactgcta tcatggtagg aaatgatcct attgctgtgg gggtttatcg
     5521 ttcacttcaa aaaaatggtc tacatattcc agaagatatt tcgattgtga gttttgataa
     5581 tattgaagtt gctgaatttt taacacctgc attaaatacg gtgaatgtga atacagaaga
     5641 aattggaaaa ctagcagttc gcatggttaa tgaacgaata aaagaagtta gaaatgtttc
     5701 gatcaaagtg attgtttcta ataatattat tgttagagaa agtgaaaaaa atatcgatta
     5761 aaaaagtaaa catccaaaaa agggtgttta cttttttgtt tactaatatt tttagtaaaa
     5821 aatctcgaca aaacattaag taaacagtta tggtgttgtt aacaaaaaga aaacgcattc
     5881 aaaacaggag gaattaagat gaaacatttc aaaaaaatag ctttaacagg gatagctgtt
     5941 ttaactggga cattactcgc agcatgtggt gggggagata gtaattctaa agacgggcaa
     6001 gtaactttaa gctatgcgag ttgagatact gaccaagctc caggactaag aaaaatgtta
     6061 gatgaatttg aaaaacaaaa tcctgatatc aaagtagaaa tggagacaac gccttgggat
     6121 caatattggg ttaaattaga agctgcaaca acgggaggta atatgccaga tgtagtaacc
     6181 atgcactcta gcgaatccta taaatatatg tcacagaatg ctttgatggg acttgatgat
     6241 gttatcaaag aaaatgatat tgacatggga aactttactg aaggaattgc tgatttctat
     6301 acgcttgaag acgaactata cgcaatccct aaagatgctt ctgtagtggg gctatggtac
     6361 aacaaagaat tatttgataa tgcaggtgtc gattatccag atgaaacatg gacatgggaa
     6421 acactcagac aagctgcaat tgatttaaca gatgaatcaa aaggcatcta tggttttgca
     6481 tcacatattg gaacagagac tggctactgg ccatttattt atcaaaatgg gggagaagta
     6541 tttgccgatg acaatacaaa atcaggtttg aataccccag aagcacaaga agctttagct
     6601 ttttatcgag atattgcact tgttgatcat gcttcaccaa caattacaga attacaagat
     6661 cctgataagt taagtcgttt tcaatcaggt agattagcaa tgatgattga aggaaactgg
     6721 tttgcaccgg gatttttgaa aaatgactat ctagttgaaa atgttgcttc tgccgtgtta
     6781 cctaagggaa aagttaatgc aacaattaca aatggacttg gttgggctgc cagtgcgagt
     6841 acaaaatacc cagaagaagt gaaaaaacta atggcatttt tagctagtga ggacgccaat
     6901 aaaatccaag cggaaacggg ggcatcaatc ccagcactaa aaggctttga agacgagtgg
     6961 gctgcttctt cagatacaat tgatttgagt gcttttggaa aaatgttgga gtatggagtc
     7021 cctcgtccat acaataaaga tggattacga gctgaacaag cagaagttca aattattaac
     7081 cgtattctgt ctggtgaagt aactgttgaa gatggaaccg ctcaaattga aaaagaagtc
     7141 aatgcgatat tgagcggaaa ttaaattagt taattataca atatacaggc gaaaggattc
     7201 tatttcgtct gtatattaat ggatgaatac caaaaagggg gatattatga aaaatcgaat
     7261 ttttggaaaa tatcgtaaag atcaagtgat atggggctat atttttattt ttccaacggc
     7321 attaggatta attttattaa atatatggcc agcaattcaa acagcaattt taagttttca
     7381 aaaaacggta gggttcggtg atactgaatg ggtcggatta agaaattacc aaaaaatgtt
     7441 tgcagacggt gaagtttttc agtctttatt aaacacatta atttatgcag tggtttcgat
     7501 acctagtatt gttgtattat ccttgttggt ggcagtttta atgaatagaa aagttaaagg
     7561 attgtcgatt tatcggacaa tttattttct accagtcgtt gctgcaccat ccgcagtcgc
     7621 aatgatttgg cgctggatgt tcaataatga ttatggactg attaataaca tgttggctaa
     7681 aataggtctc gatggaccag catggttaac ggatccatcg attgcgattt attctattat
     7741 cattgtagga gtttggagtg ctattggtta taacatggtg ttattactag ccggcttata
     7801 agaaattcct aaagattatt atgaagcagc ggaaattgat ggtgctagtc caatccagca
     7861 gtttttcaac attacgttac cattagttac accaacatta ttttttgtag ttgtaacaac
     7921 agttatcaat gctttccaag tatttgatgt tatttttatg atgattactc ctaatagtac
     7981 cgccatgttt aaatcacaat cattagccta tttattctat aaacattcat ttattttaaa
     8041 tgacaaggga tatggttcgg caatcgttat gttcctttta gtgattatat taattattac
     8101 tgcaattcaa atgaagttgc agaaaaagtg ggtaaactat taaggaggct atcaaattat
     8161 gaaaaataaa tttttttcaa atgcgccaat ccatatcatg cttattttag gtggtattgc
     8221 aatggcatta ccttttattt ggatgatttt aacttcaggg aaaacgttgg ttgaatctac
     8281 ccaaattcca cctacaattt tccctgagaa atttcagcta agtaactatc aacaagtact
     8341 tgatttatta ccattcatgg ctttttatat caatacattt ttaatggttg ttggacgagt
     8401 gattggctcg gttttatttt cagcaatggc tgcatatgct tgtgcgcgtc taagttttcc
     8461 agggaaaaat atccttttca gcttagtact tattcaaatg atgattcccg gttccatttt
     8521 tattactcca cagtatttat tagcacaaaa attgggtatt ttaaatacaa ttccagcatt
     8581 aatattacca ggggtagtta gtgcgtttgg tacgttttta ttaagacaat ttttcttagg
     8641 tttacctgat gaattggagg aagcggccta tttagaaggt gccaatattt ggacgatctt
     8701 tttcaaaatt atgttgccgc ttgctaaatc aggattggtt gcacttggaa tattcacagc
     8761 gttgtttgca tttaaagatt taatgtggcc attgattgtc aatacttcaa ttgataagat
     8821 gcctttagct ccgggattag caaacttaca aggacaatat gccacgaact tcccacaatt
     8881 aatggcaggt tcgttattag caatttggcc aatagttgtg atattcatcg ttttccaaag
     8941 aaaattcatt gaaggaattg ctacaacagg tagtaaatta taaaactatt aattagtgat
     9001 tggagagtaa gtacatgaca aaaattacat ttattggtgc aggaagtact atttttgcaa
     9061 aaaacgtatt aggcgatgcg atgttaactc caagtctaca agatgcacat attgcattgt
     9121 acgatattga tgaaaaacga ctaaaagaat caaatttaat gcttaaaaat attaataaaa
     9181 attcgaatga aaatcgtgct gatattgttt cttattatga tcgcaaagaa gcgttaagag
     9241 aggctgattt tgttatcaat gctattcaag tgggtggtta taaaccttca acagtgattg
     9301 attttgatat tcctaaaaaa tatgggttgc gacaaactat tggtgacacc atggggattg
     9361 gtgggatttt tagaacccta cggacactac cagtaatgtt agactttgct aaagaaatgg
     9421 aagaagtttg tccaaatgct tggttattga actatacaaa tccgatggcg atgttaacca
     9481 tgggtgtttt aaaagcgacg agtattaaga ctgttggttt atgtcatagc gttcaagtat
     9541 gcgtaccaga attgtttgag catttaggaa tagccgatca atatgactta aatgactttc
     9601 aatggaaaat tgcaggcatt aatcatatgg cattcttgtt ggaaattaca aaaaatgggg
     9661 aagatttcta tccaacaatt cgtcagttag ctgcagaaaa accaaatcca catccagatt
     9721 ctgtacgttt tgaattgttg aaacgttttg gttactatat tacagaatca agtgaacaca
     9781 atgcagaata ccatccatac tttattaaat caaaataccc agaattgatt gacgaattag
     9841 ggattccgtt ggatgaatac ttacgtcgtt gtgaacaaca aattaatgat tgggaaagca
     9901 tgcgagagga cattgtaaat aatggggagt taacacatac gagaagccgt gaatatgctt
     9961 catatatcat ggattctatt acgacaggaa ctccgtcagt gattgcagga aatgtcttaa
    10021 ataaaggctt aatcacaaac ttaccgacag atgtttgtgt ggaagtacct tgtttagtcg
    10081 ataaaaatgg cgtgcaacca acctatgtcg gtgaattgcc aacacaatta gctgctttaa
    10141 atcggacgaa tatcaatgtt caagaactga cagtagaagc cgctttaaca ctagataaat
    10201 caaaaattta tcaagcagcg tatttagatc cacatctatc atctgaattg tctttaacag
    10261 atatcaaaaa tttagtggat gatttaatcg aagcacatgg ggactattta cctgaatata
    10321 attaaaaaga agctgaaaga gtaaggaatt ttcaattcct tactcttttc ttagttaaat
    10381 ttagtaaaaa tattgacaaa acacttagta aacagttaca ttagtgttaa caaaaagaaa
    10441 acgcattcaa attgtataag gaggagcata aaatgaagtg gtctaaaaaa attattttag
    10501 cttgtatagc actaggagca acgatctctt tgactgcttg tggaaaaaat caggaagatg
    10561 caagcggtaa ggtagaaata gagtttttta atcaaaaaaa agaaatgact caaacgattc
    10621 aggagattgc taaagatttt gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg
    10681 taccgaatgc tggtgaagta attaaaacac gtatgcttgc aggcgatgta ccagatgtca
    10741 ttaatcttta tcctcaaagt atagaattga aagaatgggc gaaagctggt tatttagagg
    10801 acttaaccga agaaccgtat ttagagaata tcaaaaatgg atatgcacaa cgttttgcta
    10861 ttgaggatcg tgtatatagc ataccattaa ccgctaatgt ctatggtttt tactataaca
    10921 aaactgcttt tgaggaaatg gggattcaag caccagaaac ttgggcagaa tttgaaaaaa
    10981 tagtagctga gattaaagat caaaacaaag taccgtttgc gattgcagga gcagaaggct
    11041 ggactttaaa tggttatcac caactagcta tagcgactgc tgctggtggt gaagaggaag
    11101 cgaacaatgt ttggcgtttc tcagaagtta atggaattaa tgctgaaagc aaagaaatgc
    11161 aaaaagattt taatcgatta gatttgttaa gagaaccaaa agctttgcaa aacaactggc
    11221 aaggtgccgg ttataacgat acagtagtta cttttacaaa aggtgatgcg ttgattatgc
    11281 cgaatggttc atgggcaatg ccgatgattc attcacaaaa tccagatttt gaagtaggaa
    11341 cttttccttt tccagcagat gaagcaggtc aaagtctaac gattggcgca ggagacctag
    11401 ccttatcagt ttctgcaact tctcaacata aagaagaagc caaacgtttt gtagaatata
    11461 tgacaacacc tgaagcgatg cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg
    11521 agggcgttat cgagaatact caggattcac cattaagtgg tttaactgaa cttgctttta
    11581 ctgatcgtca tttagtttgg ttagccaaag attggaatag tgaaaatgat ttttatacct
    11641 taacaacaaa ttatttacac aatggcaatc aaaaagccat gattaacgca ttaaatgctt
    11701 tctttaatcc aatgaaagct gacggaaggt aggaaaataa aatgaaaata aaagaaattt
    11761 taaatcgtta ctgggcaatc atttttgtag caattccaat tattttgcag atgatttttt
    11821 tctactttcc aatggttcaa ggtgcttttt acagtttaac taattggact ggtctgactt
    11881 ataattttga ttttgtgggt ttgaacaatt ataagattct tttttctgat ccgaagttta
    11941 tcaaatcgat tggcttcact ttaattgtga cgctttgcat gattgctgga caaattggtt
    12001 ttggtatttt gattgcgcgg gcgttgaacg caaatattaa agggaaaaca ttctttagag
    12061 catggttctt ttttccagcg gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa
    12121 tttttaacta tggtttacca aaaattggta gtatgttagg cattgaattt ttacaaacca
    12181 gtcttttagg aacaacatgg ggagcgattt ttgcagtaat ttttgtttta ctttggcaag
    12241 gaattgccat gccagtcatc attttcttag cgggttttca aagtattcca caagaaatta
    12301 ttgaagctgc tagaatggat ggggcgaatg ataagcaaat tttttggaat attgaaatca
    12361 cctacttatt accaagtatt tcaatggtat ttattatggc attaaaaggt ggcttaacag
    12421 catttgatca aatttttgct ttaactggtg gtggaccaag cgatgccaca acatctttgg
    12481 ggttacttgt ttataattat gcttttaaaa acaatcagtt tggttatgcg aatgctattg
    12541 ctttagtctt atttatctta attgggattg tttcactggc acaattaaaa ttatcgaaaa
    12601 aatttgaaat ctaggaggtt tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata
    12661 cttgctttta ttattagggg gcttcatgat tttagttccc ttagtttcaa cgctattcgg
    12721 ttcatttaaa acaacacccg atattatgaa taatttcttt cgttttccta atccagcgac
    12781 tttggaaaat ttccgaagat tgttagcgga tggaattggt cattactttt ggaattcggc
    12841 aatcatcaca atcattgcag tagttgctgt gacagcaatt attcctatgg ctgcttattc
    12901 actagcaaga aaaatgactc gtgggaaagc atacggattg atgtatttat ttttaatttt
    12961 aggaattttt gttcctttcc aagtaattat gattccgatt accgttatga tgagtaaaat
    13021 tggtttagca aatatgtggg gattaatctt actctatctc acttatgccg taccacagac
    13081 actattttta tatgttggtt ttattaaact aaatgtgcct atcagtctgg atgaggctgc
    13141 tgaaattgat ggggctagtc actttacaac ttatttcaaa attgttttcc caatgttaaa
    13201 accaatgcat gcgacgacta ttattcttaa tgctctatgg ttttggaatg atttcatgct
    13261 accattatta attttaaatc gtgattctaa tatgtggaca ctaccattgt ttcaatataa
    13321 ttatacggga caatatttta acgactatgg tccaagtttt gcttcctata cagtaggaat
    13381 tgtcacgatt acactagttt atcttgcttt ccaaagaagt attatagctg gaatgagcaa
    13441 tggagcagtg aaataaccaa taaattattc tcattataaa ggagttcata aacttatgat
    13501 tatatttgac aatcacacac aaacatttca tttacaaaat aaaaaaatta gctatctatt
    13561 aggaatcgaa gaaaataatt atttaacgca tctttatttt ggaaaaaaga ttacgcacta
    13621 ttctggtggg tatcgctacc cccgaacaga tcgttccttc tcacccaaca atgcaaatgc
    13681 gcaagatcgt ttattatctt tagacacatt accattagaa tttccaggat atggacatgg
    13741 tgattttaga gaaccggcgc ataatattaa attaggaaac ggtagtcgtg taaatgattt
    13801 tcgttatgat agctacgaaa ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt
    13861 atatacggaa gatgatgatg aagctgaaac gctgattatt caattgaaag acgcgattag
    13921 tggattgact ttacagttgt tctacacaat ttatcaaaat cacagtgtaa ttattcgttc
    13981 tacgcaatta gtaaataata gtgatgaaac agtggagatt aaccgcttag ctagtcaatc
    14041 aattgatttt ccaaatcgtc cactcgagtt aattcattta aatggtacct gggcaagaga
    14101 acgtcaaatg actagagaaa aaattcatac agggataaaa gtattagata gtaaacgcgg
    14161 atcaagcagc catcaacaaa atccgtttgt tgctttagta gatgccaaaa cagatgaatt
    14221 tcaaggagag gcatatggat ttaatttagt ttatagcggt aatcacgaaa cggtgattca
    14281 aaaagatcca tttgaccaaa cacgggtcat tatgggaatt aattcattta attttggttg
    14341 gcaactaaaa actggcgact catttcaaac gccagaagtc gtgatggttt atagtaatga
    14401 aggtctgaat acgttatcac agacgtatca tgaattgtat aatcaccgtt taattcgtgg
    14461 aaactatcgt ttaaaagaac gtcccacgtt aatcaacaac tgggaagcaa cgtattttga
    14521 ttttaacgaa gaaaaaatca tgaatattgt cgatgatgcg aagaacttgg gcattgaaat
    14581 gtttgtttta gatgatggtt ggtttggcgg tcgtgaaaat gacttcactt cattaggtga
    14641 ttgggttgaa acagaaggca aacttgcttg tggtttggaa aatttagcca agcaagtgca
    14701 tgataacggg atgaaatttg ggatttggtt tgaaccggaa atgatttctg aagatagtga
    14761 actatttcga gcacatccag attgggcatt gggtattcca ggtcgtgacc gctcaattag
    14821 tcgaaatcaa tatgttcttg atttttcaag acaagatgtt cgtgataata tctatcaaca
    14881 aatgacggcg atttttgatc gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa
    14941 tatgacagaa gtctattctg tcttgttgga ctcaacgatg caaggtgaag tggctcatcg
    15001 ttatatgtta gggttatatg aatttttaga aaagttaaca acgaattatc caactatttt
    15061 atttgaaagt tgttcgggtg gaggcggtcg ttttgatgca ggtttcttgt attacatgcc
    15121 acaaacttgg acgagcgaca ataccgatgc cgtggctcgt ctgaaaattc aatatggaac
    15181 cagcttagtg tatccaattt caagtatggg tgcgcatgtt tcagcagtcc caaatcatca
    15241 aacaggaaga gaaacgagtt taactattcg tggcaatgct gcgatggctg gtgtttttgg
    15301 ttatgagcta gatctaacgc aattaacttc tgaagaaaaa gcagaaatta aagaacaagt
    15361 tgccttttat aaaaagcatc gccaactttt gcaatatgga actttttatc gcttggaaag
    15421 tccgtttgaa ggcaatgata ctgcatggat gtttgtctca aaagaccaaa cagaagccat
    15481 ggtcttttat ttccgagtat tagcagaagc tgcgcatcca ttagtcacat tgaaattagc
    15541 aggattagca gaagaagcaa cttatcaaat ggaagatgta acatttgctg gcgatgaatt
    15601 gatgaatctc ggtttttata ttgactcaga actacatggt gattatgcga cacaacgctt
    15661 ttatttgaaa cagatatagt agcttgaatt tacaatataa gtgcaacacc aaaagagact
    15721 cattccaaac gaagaaaata tgttttattt acacgcattt actaaaaagc agcctgattt
    15781 gaattgggaa aatgaagaag tacgtgaaga aatctatgca atgattaact attggttaga
    15841 taaaggacta ggcggattta gaatcgatgc gatattaaac atcaaaaaaa aactagagta
    15901 tggcatcttt gaatctgatg gagaagatgg gttagccttc attgggccct ggattcttaa
    15961 tcagtcgggc attgaagtgt ggttaaaaga aatgagagag cggacattta aaccacataa
    16021 tagtatgacg gtagcagaag ccgatgttcc taatgaacgt ttagatgaat atattggtga
    16081 agatggctat tattcgatgg tttttgattt tagttatacg gatattgatg tacctgaaac
    16141 tggtgaatgg tttaaagata gccaatggac atggacggat atgcgtacca atatttttac
    16201 gaatcaatta gttacgcaag ataaaggttg gggcgcctta tatttagaaa accacgatca
    16261 accacgttca attaataaat atattccaga agaatggatc aatgattata gtaaaaaaat
    16321 gttggcgacg ctttttatgt tactaagagg tacccctttt atttatcaag gacaagaact
    16381 aggaatgacc aacatagaaa tggcatcatt agaagatttt gatgatgtag ctacgcattc
    16441 tcagtataaa cgggcattag aatatggatt ggcaccagag caagcattga aagctgtcaa
    16501 taaacgaagt cgggataatt ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag
    16561 attctcaact tctgataatg tatggttaaa agtaaatcct aattatccag aactcaatgc
    16621 agaagctcag ttaaccaatc aacagtcggt attcaattat tatcatcaat taattaaatt
    16681 aagaaaaaat agtgtataca aagacgtgct aatctacgga caatttctac cagtgaaaga
    16741 taaaaatgaa catattttgc tttatgaacg ccaattagaa gataaaatcg tattagttat
    16801 cttaaacgtt acagcaacag aacaagaata ttatgtagat gaacagtacc aacaagtatt
    16861 aatcaataat tatgaagatg ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt
    16921 tgaaagtatt gtattagcta attatggagg acaattatga aaattaaaaa cgaagcaatg
    16981 ctcatcacct atgcagatag tttaggaaaa aacatgggcg aattaaaaca agtcttagac
    17041 aaacatttac aaggtgttat tggtggtgta catctgttgc ctttttttcc atcgacaggt
    17101 gatcgaggct ttgcgccgag tgattatacg acagttgatc cagctcttgg aacatgggaa
    17161 gatgtggaag cattaggtga agattattat ttgatgtttg attttatgat taatcacatt
    17221 tctcgtgaat caaaattttt ccaagatttc aaaaaaaacc atgagcagtc accatacaaa
    17281 gaaatgttta tccgtattca tgaatttttc ccagaaaatc gaccaacaca agaagatatt
    17341 gatttaatct acaagcgtaa agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt
    17401 acgacagaag aggtttggaa tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa
    17461 gaagttgtga aagaatttat tcgagaaacc atcaaagata tggcgaacca tggctgttct
    17521 ttaattcgat tagatgcgtt tgcttatgcg attaaaaaat tagataccaa tgatttcttc
    17581 gtggaaccag atatttggga acttttagac gaagtgcgtc aagaagcagc gaaatatgac
    17641 gtcgaattat taccagaaat ccatgagcat tattccattc aaatgaaaat tgccaatcat
    17701 gattattata tctatgactt cgccttgcca atggtcactc tgtattcact ttatagtgga
    17761 aaatcagaac gtttagccaa ttggttgaaa atgagtccga tgaagcagtt tacaacattg
    17821 gatacgcatg atggaattgg tgttgtggac gctcgtgatt tattaacaga tgaagaattg
    17881 gattacacct ctgaggaatt gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt
    17941 gccaattaca ataatttaga tatttatcaa attaacagca cctattatag tgcattaggc
    18001 gataatgacc gtagctatct gttagcacgt gcgattcaag tatttgcgcc agggattcca
    18061 caaatttatt acgttgggtt attagcagga aaaaatgata ttgaactgtt ggaagcaacg
    18121 aaagaagggc gtaatattaa tcgtcattac tatgatttag ccgaaattga agaagaagtg
    18181 caacggccag tgattcaaca attatttgca cttttaaaat ttagaaatga atctgcggcg
    18241 tttgatttgg atggaacgat tgaagtagca acgccatcag aatcagaaat agtgattaca
    18301 cgtaaaaata aaaacggtag tgatagtgca acattaacgg ctaacttgga tacgaaagaa
    18361 tttatcattg agtctaaaga tataaaaatt ttcactagtg ttgcattaaa attttaacct
    18421 attccataga acaaaaaatc ctttatacta gcacttggat gacttatcca agaccaataa
    18481 aaaggactca tacatggata agtataaaac aaatacagca tttaataaat ggttttctgc
    18541 cataaaatta ggaaaacttt ctgtcaactc ccaaaaatgt atttttgatt acaattcgta
    18601 tcgtaaaaaa ctgtcttttg aagctgtgct caagctcttt ctctacgcag ttaatgaaga
    18661 aaaagaaagt ttgcgtgatt tgagtacagc tcttatcgac cgatcgctcc aactagaaac
    18721 cgatgtgtca tcgattagtc atacccaatt gtctcgagca tttaatgcac ttgaacctaa
    18781 agtgttagaa gaaatttttc aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac
    18841 caagcgaaat catctgtatc tgattgattc cagtacgttt tcgcttagtt taaaacgtca
    18901 taagtgggcg actttccgtc aaacaaaatc aggtgtcaag ttacacttaa actattgtta
    18961 tatggacgat actacgatgt atccaactga ttttacctta acaaatgccc atgaacatga
    19021 tctcaatcaa ctaccagtat tggtgaatca accagaggca acttacgtgt ttgatcgtgg
    19081 gtattaggac tttgaaaaga tggatcagat gcattgggat ggctatttct tcgtcactcg
    19141 gattaaaaaa aacaccaaag ttcacgtgat agatactctg gaaacatcgc cagaaacaga
    19201 aattctacga gacgagctgg ttcgtttagg atctaaaacc tatctaaccg ccaatttccg
    19261 actagtcact gttcaagata agaacggacg tgtgtttcaa tttatcacca atcgaatgga
    19321 cgtctcttca aaagaaatct ctgatatgta tcacgctcgt tggcaaatcg aattgttttt
    19381 caagcacatc aaacaacaca tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt
    19441 gcagaatcaa ttgattttaa cgatgatttc cgctctcctc acgtttctca tcaaattgga
    19501 gacgaaaacg gaaaaatcag tgttccaaat caaacgattc tttcgttatt tactgtttca
    19561 gccctttgaa tgttgtctag aaaaattaat tcccacatga gaaaaaagaa atttggctcg
    19621 ttacttttgg ttttcagttg aataacagat aaaaaatttt ctggaaaaag tcatcctctg
    19681 cgtcagcaca gttttttttg acttattttc agaggaaata gttttcagaa tattctaaaa
    19741 tgttttatgc aacactagtg atatttttta gaattagatt tctaatgtgc attttttgag
    19801 tattaaggag caagtataca aagatttata aagctgatta aatttgaata aaaatatgca
    19861 ggaacgctac tatttttagt agtgttcttt tttatatttg aataaaaaat gtggtggtta
    19921 aatggtggtt ataacaaaaa atgaattaat gaaattaggt tatgggaagt atcagtctga
    19981 gagaatcata cgacaggcaa aagcaattat ggtttcaaaa agctatacct attacgaaaa
    20041 tagaagatta ggactagtac ctgttgaatc tgtagaagaa gttttaggta tgaagataga
    20101 ggtagaggag gattttcaga atcatggtta attttaaaaa agataaaaat gggaaatatc
    20161 attttactgc tagtttggga ttcgacaaac gaacagggaa aagagtacaa aaaaacgtag
    20221 aggggttaaa acagttaaag aagaaaaaaa gcgtataccg aacttgtttc taattatgga
    20281 aaagaagcag aaacttctca aagtactatg ttgtttgagg agttttttta taaattattc
    20341 ctaccttatt atcaagggaa agtagaagaa agaacatata aaaatcggtt atctacaatc
    20401 aaaaaaactt ttcttatttc tttaaaatgg agttaagaga aatacgtcca attgacattc
    20461 aaaagtggca aaataattta ttgagcaaat ataaaaattc ttatgtaaga aacatgtatg
    20521 ggatttttca gatgtcatta gatcgagcag ttgttttggg actacttgaa aaaaatcctg
    20581 caaaagttat tggaaatgtg aagaaaagga aaactacagt tgatttttgg acaaaagaag
    20641 aatttacaaa agttatttca actttttata ttgatgattt gtaccaacat tttggtttta
    20701 tttgtatgtg gttacttttt atgaccgtta tgcgtttggg agaagccact gcattaactt
    20761 ggaaagatat tgattttgaa aataaaaact tgcgtgtgaa taaaacactc tattataaga
    20821 gagccaaaac ctatgagttt gtagaacccg aaacggcttc tagtattcgt acaatcgaac
    20881 tagacgacaa tacaattacc tttttaaaag cttggaagga gcgtcaagtt tcaataggag
    20941 gcatgtcgtt tgtactttct tataatggaa cacctactca gaaatataca atccgtcata
    21001 ttatgagaag acatgcaacg atcgttggtg ttcatataat ccgcatacac ggattaaggc
    21061 atagtcatgc ttctttgctt atatcacaat aaaaagatgc acttcgaatt aaagaaaggt
    21121 taaaacatgc tgatgtacaa acgactttag gaacttatgg tcatttgtat ctaaattcat
    21181 tgagagagat aacaaatgat cccacagatt atatccaaac taatttttct aaaatcaaac
    21241 cgacacctat ttacgataat aaaaaataaa tgataaacaa aagtaggatc acaccaaaag
    21301 aaacacacta gaaaaactat aatacaggct tttctagcgt gttgtttctt atttccgcct
    21361 atttatacat tatactcata aagcatttaa tgcatcgatc gtagccgcat tgtctatatg
    21421 ggtatataaa gttgttgtat tggtacttga atgtcccaat tgttgggcag ttaataccaa
    21481 tgaatttgtc tgttggtaga gtctagtagc taatgtatga cgcagtttgt gagggctgac
    21541 ttgaattcta aagccttttg aatattttgc taccatccgt tcgattgcaa taccggatat
    21601 acgcttggct tctccttggt aaattgctag aaataaagct ttttcgtttt ctgttacttt
    21661 atatcgttct ttccgaatag ctagataatt agctaaatat tccattgcga atgctgctat
    21721 attgacagca tctcttttat ttccttttct agttactacc acattattct tatctaaaga
    21781 taggtcatct aaattaatat ttataacctc ggagagtctc aatccagatc ctaaaatcag
    21841 tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttact
    21901 caaagtattt ttatattcat tggcaatata atctagataa ttttgtgtat ccttatctag
    21961 aaataatttt ttctcaatgg cattggcacg tgcagctaac gtagttttat ctgtgatggt
    22021 tgctacttga tccataggat tattgtctaa gtatggtttt cctagaggat tattggaact
    22081 tttcgaaaga tatttaaata gaacctttaa tgccgtgata tttctattga tagtagaagt
    22141 agatagacct aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt
    22201 ttcttttgtg cgacgtttca aatgagattt gtacaagttt aggtcatagg cagttaactg
    22261 tcttatcgag gtgatagaag tatcaggaac gatagactca ctcagaagcc attcataaaa
    22321 gagcttataa ttttttagat attctaaagt ggttcgaggt gattgatctt tatcctcacg
    22381 atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg
    22441 atatatttct gtatttttca tgtcattcat cctttcaata agtaggggag aagagtatct
    22501 ctataattgg actataccaa ttatagaatg ttttgtcaat tggtatgctc taaaatttat
    22561 taatttgttt caaaaaaagc atataaacag gtatacgttt aataatttaa aataaatggt
    22621 atacataaat agggaaggaa atagaaggga gcaatctaaa ttttttgaat tttcttaatt
    22681 aattatatag aaagaacaat ctttctatag agttagacat gagagggact atgcttgatt
    22741 taaaatcaac aagcgtatat actgtatata caaagaaaaa ggagggagaa gactatggca
    22801 attaaagaaa gaaaatttag ggaagtgggg aactcagtag tattaactat ttcaaaggag
    22861 tttcttaaag aaaatgggtt aaatcccgga gatacggttt ttttagatga agaaaaactt
    22921 cgagcggcaa ttacaaaaaa acaagcctta tctgatgaaa ttgatttagc aatatctcaa
    22981 gcattaactg aatatgatac tacttttaga gagcttgtcg ataaatgagt attcattacc
    23041 tagaagccaa agatattata aaaatgaatg taatccaaat aaaaaaatac tctcctaatg
    23101 aaccccttgg tgtaaaggat gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct
    23161 cagcttttgg tgaagaggtt tatccttcga tttatgaaaa agcagctatc ctacttatac
    23221 aattgataaa gaaacatcct tttcacaatg caaataaacg tactgctttc ttagctactt
    23281 ttgtcttttt gaaaatcaat ggacagcttt tagctattga tcaaaaagat gctgtaaatt
    23341 tagcggtata tattgctacg tatgataagg atttcgatca actaaaatat gaagtgatca
    23401 acactataaa gacacatacg cagcccattt aaggagttac tgtatgtgtc tttatagggt
    23461 tattagctgc tatagcgaaa taattgaagt aaattacaaa tagctataat tcaaatccaa
    23521 ctatatagaa aacgtaaatt ttctatatag ttggattaag ataacgacta tttattttat
    23581 catattttta ctatacccta tacttttact tggtatattt atgaataatc gcaaatttac
    23641 ccctatacta ctagtttgta agttagatta ataaaaatac taaagttatt tgttctttta
    23701 ttaaatacac ctacaactta taaattgatg gtatttagat aacacgaaag aataaaaata
    23761 acagctgata gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt tagtgcgtat
    23821 gaacttgctt ctgaaaactt atgaaatgaa agacctttta catcttgcag aaatcggtac
    23881 tgggattaaa ctaagcaata aaaactaatg ttcccctcat tctttgaact tctactattt
    23941 gatttattta ttctgataaa tgacgatttc accttcagga tcttcaaatc ccgtatagaa
    24001 tttatttaag atctcttcag tatcagtaat aggataggcg ataggccaca taccctttga
    24061 tggttagaat aaactgctca aaatctgtat cgtatgttgg ctttagcata ttaacaaagg
    24121 ctatctccaa attataaacc ataagcgttg aatcgttctt aacaatttca attcttccta
    24181 acccaaagtc agaagatata attaccggac ttgccttacc cttcatgtgg gttgttgaga
    24241 ttccatattt aaagggaatt gtcggtacta tcggcggtct gaaaaacatc ccaaaaataa
    24301 aaaaatcttt cccctgctag tctcctaagt ccctttgtag cggatatggc accataatct
    24361 cattttatcg aagtgagtca tggtttatct gttggccaac caaaattgat acaatctaac
    24421 gatgcaccct ctacggcatt ctaggggagt gcacttgcat tcacccaata aaaaccggca
    24481 gttagcaaag aacttatgta gtcgtatgtt cttactgtta acatacaaga ggtctccttt
    24541 ccacaattgc ttagcagtaa acgggaattt acaaagctaa tctcttccat tttcttatct
    24601 ttgttctaaa agtcccaaat ggagcaactg tatttacatg aataaattta tatacttccc
    24661 atacagccgt tttggttgcc tcgtctgccc attttctttt gtgtgcagtg aatagatctt
    24721 catcactcat tgaatcaatc atcatgttaa tatcatcaat attacgattg agaatttcct
    24781 ctaattcttt caatggtaga tgtgcatagg tatttgtaaa ccacttatat aactctccta
    24841 actgattcca tttaaattga tcagaaggcg ttttaacaga taatccattt ctttcatcat
    24901 tttcccattt tagaattaaa ttcgtccaac caacttgata tgctaaattt tcagcaggtg
    24961 ttctatccac ttcctcaact cttttatcct tcaattcttc aggaatagtt tcaaattcta
    25021 aaatatattt agtataagtc ctatttattt ctttttttaa ttcttcttta tttttatatg
    25081 ttctcattat attacctcca taaattccta attgtctcct ttaattcatc tataatttca
    25141 tccacgatat tttaatccgt tctacaatgc atatctgata tgcattgact gtctaactca
    25201 tatgtgaatg atttataaaa tatcccaacg tctcatctaa agaaatgttg atcgtcagca
    25261 aggagctaaa aaatacgaat atatttcttt tgtagtaaaa tcggactggt ttgcatatca
    25321 ttttcttcgt aaatatcaaa atcatgttaa ctactatcaa ttactaataa aaactaccac
    25381 tttaagcgtt aagaactgtt aagtttagtt aagtgaggat agttcacaaa atccattgtt
    25441 aaacttcacg aaagtggtaa aactcaaaat gctttatata ggattacagc atcgctcttt
    25501 cttcgattgc gcgttgggta aaaaatatta tgaagtaaaa attgacgatg acacgttcct
    25561 tacggctcat cagatgaaac agctacaaaa acgaaatgat caactcgaag aaaatttaat
    25621 tctaaaaaag cgattgccat attcacgcct cgctcgacga actattaaag gtcaagattt
    25681 acctgaagat catccttttt aggaaattga agctttttat gttgcacaat gcatttataa
    25741 tactacaatg atgttttcac cagatattct tctagggtgc ggtgtaatga aacagataca
    25801 cttgcaaaag aaaattcacc gcgaatttac taaaattatg aataattatg ttaatcttcc
    25861 gaaatttttt tccaaaatta agggatcaag ctggtgttat agattgttta aggttggcaa
    25921 aaaaataact tctttttaaa caattataga aaaccgatac tctgttaagt tggttgacac
    25981 ggtgtcgatt tttatgactt tcttattctg aaacttcact aacggacagt acctaaaaag
    26041 tctatagtat ggacgtttac gagtactatt ttatcaaaaa tgctgctcat ttcaacttat
    26101 atgttttagt gaactacccc cacttagctt ggataggcta caattaacta gacagaaaaa
    26161 ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct aagagaacac
    26221 gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta gctggaaagc
    26281 cacgtgtaga aatcattcga gaatatgaac taac
//
LOCUS       DANJLF010000033        26006 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000033 DANJLF010000000
VERSION     DANJLF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            117..929
                     /locus_tag="PWT25_001542"
     CDS             117..929
                     /locus_tag="PWT25_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL1021632.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            926..1726
                     /locus_tag="PWT25_001543"
     CDS             926..1726
                     /locus_tag="PWT25_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL1021633.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1887..3155
                     /gene="rseP"
                     /locus_tag="PWT25_001544"
     CDS             1887..3155
                     /gene="rseP"
                     /locus_tag="PWT25_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL1021634.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3223..4932
                     /locus_tag="PWT25_001545"
     CDS             3223..4932
                     /locus_tag="PWT25_001545"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL1021635.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5144..9502
                     /locus_tag="PWT25_001546"
     CDS             5144..9502
                     /locus_tag="PWT25_001546"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL1021636.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9647..10120
                     /gene="rimP"
                     /locus_tag="PWT25_001547"
     CDS             9647..10120
                     /gene="rimP"
                     /locus_tag="PWT25_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL1021637.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10143..11318
                     /gene="nusA"
                     /locus_tag="PWT25_001548"
     CDS             10143..11318
                     /gene="nusA"
                     /locus_tag="PWT25_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL1021638.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11340..11633
                     /locus_tag="PWT25_001549"
     CDS             11340..11633
                     /locus_tag="PWT25_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL1021639.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11630..11941
                     /locus_tag="PWT25_001550"
     CDS             11630..11941
                     /locus_tag="PWT25_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL1021640.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11954..14260
                     /gene="infB"
                     /locus_tag="PWT25_001551"
     CDS             11954..14260
                     /gene="infB"
                     /locus_tag="PWT25_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL1021641.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14287..14634
                     /gene="rbfA"
                     /locus_tag="PWT25_001552"
     CDS             14287..14634
                     /gene="rbfA"
                     /locus_tag="PWT25_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL1021642.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14791..14964)
                     /locus_tag="PWT25_001553"
     CDS             complement(14791..14964)
                     /locus_tag="PWT25_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1021643.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15412..17774
                     /locus_tag="PWT25_001554"
                     /pseudo
     CDS             15412..17774
                     /locus_tag="PWT25_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18142..19065
                     /gene="truB"
                     /locus_tag="PWT25_001555"
     CDS             18142..19065
                     /gene="truB"
                     /locus_tag="PWT25_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL1021644.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19069..20010
                     /gene="ribF"
                     /locus_tag="PWT25_001556"
     CDS             19069..20010
                     /gene="ribF"
                     /locus_tag="PWT25_001556"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL1021645.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20362..21147
                     /locus_tag="PWT25_001557"
     CDS             20362..21147
                     /locus_tag="PWT25_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1021646.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21160..22011
                     /locus_tag="PWT25_001558"
     CDS             21160..22011
                     /locus_tag="PWT25_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL1021647.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22135..22587)
                     /locus_tag="PWT25_001559"
     CDS             complement(22135..22587)
                     /locus_tag="PWT25_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021648.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(23052..23420)
                     /locus_tag="PWT25_001560"
     CDS             complement(23052..23420)
                     /locus_tag="PWT25_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL1021649.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23570..24052
                     /locus_tag="PWT25_001561"
     CDS             23570..24052
                     /locus_tag="PWT25_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL1021650.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24089..25099
                     /locus_tag="PWT25_001562"
     CDS             24089..25099
                     /locus_tag="PWT25_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL1021651.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25214..25927)
                     /locus_tag="PWT25_001563"
     CDS             complement(25214..25927)
                     /locus_tag="PWT25_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021652.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         8834 a         4483 c         5602 g         7087 t
ORIGIN      
        1 aaagaaaaaa ttattcagtt tttaaataaa aaaacaatga tttcatagga aaaacagtta
       61 gatattgcta taatacttaa gatagtggga atcttttaag tataggagga aaaagaatgt
      121 tacgtttttt tccgcaaaaa aacaaatata ttcaagaaaa aagcgaattt caatttaata
      181 ccgatggacc aattcctcag cacattgcca tcatcatgga cggcaatggt cgttgggcgc
      241 aaaaccgtcg tttgccacga gttgctggcc ataaagaagg catggaaaca gtaaagaaag
      301 tcacaaaaaa agcatcaagg cttggagtga aagtgttgac gctttatgcc ttttcgactg
      361 aaaactggaa gcgaccaaaa gatgaagtaa gttttctcat gcaactacct gtcgacttct
      421 ttgatacgtt cgtaccagaa ttgattaaag aaaatgtcaa agttcatgtc atgggttatg
      481 aaaacgtatt gccagaacat acgcaagatg ctgtaagacg tgcaattgag caaacaaaaa
      541 acaatactgg aatggtattg aattttgctt tgaattatgg aagcagagca gagatcgtga
      601 ctgctgtcca agaaattgcc gaagaagtag caaaaggaga aatacacgca gaagaaatcg
      661 atgatgaatt gattgctaag catttaatga caggtttctt gcctaaagag ttacaagatc
      721 ctgagctgat gatccgtaca agtggagaag agcgaattag taattttctg ttatggcaga
      781 tcgcatatag tgagctgtat tttacaaaag cgctttggcc tgattttgat ggtgcgcatc
      841 ttgaagaagc aatcgcctct tatcaaaatc gagaccgtcg attcggcggt gtaaaaaaaa
      901 cagaagatga gaaaggggat cagtcatgaa acaacgtgtg gtcacagcag ccgtggcgct
      961 agtcttgttt attccgatta tatggatcgg aggcatagcg gttgagctta cagcagcaat
     1021 cttagcagta ataggagttt acgagctgtt tcggatgaaa ggattgacac tcttaagttt
     1081 tgaaggagtt ttatccgccg ttggtgcagt gttacttgtt ttgccaaaag aaagatggtt
     1141 tttctttctt ccagagaatg taagtacatt tatgttattt tatcttatag ttatgattct
     1201 tttaggaaca tcagttattt ctaaaaatac gtatacgatt gatgaagcag gttttcctgt
     1261 gcttgtcagc ttgtatgtgg gaataggttt ccagaatttt gtcaatgcac gaacagaaag
     1321 cttggctgtt ttgatatttg gtctatttat tgtttgggca actgatatcg gagcatatat
     1381 ggttggtcga cgttatggtc agcataagtt aagtcctgat atttcgccca acaaaacgat
     1441 tgaaggtgcg ctcggtggta ttggtagtgc tgttgtagtc gcatttcttt atttattgct
     1501 atacccagca aaagaactat ttaatcatgg tatgtttgtc atgcttctga tgacgattat
     1561 tttttcagca gtcggacaat ttggtgattt ggtcgaatct gcaatcaaac gtcattatga
     1621 tgtgaaagac tcgggaaata tcttaccagg ccatggtgga atcttagacc gtttcgacag
     1681 tttgctgttt gtatttccta tcatgcattt atttggcatt atttagtaag tatacctgcg
     1741 tttctttgtg aatttcataa aagacgcagg tttttatttt tcattatatt gatactattg
     1801 gtgtctttgg tatactatgg taaaattata gtgttttatc gttgttttat aaaaaacaca
     1861 aaaaacaagt aaaggagaaa aaagctatga aaacgattct gacatttatc atcgtttttg
     1921 gtatattagt gattgttcat gagtttggtc atttcttctt tgcgaaacga tcaggaatcc
     1981 tcgttcgcga atttgctatc ggtatgggtc cgaaaattta tgggcatcaa gcaaaagacg
     2041 ggacaacata tacgcttcgc ctattaccta ttggcggtta tgttcgaatg gcaggcaatg
     2101 gggatgatga aacagaaatg gcaccaggaa tgcctctttc tcttttacta aattctgacg
     2161 gcatcgtaga gaagataaat ttaagtaaga aaatccaatt gacaaatgct atcccgatgg
     2221 aattgagccg ttatgattta gaagatgagc tgactatcac tggttatgtg aatggagacg
     2281 aaacagaagt agtaacctac cctgttgatc acgatgcgac gattattgaa aacgacggaa
     2341 cagaaatcag aattgcgcca aaagacgtac agttccaatc ggctaaattg tggcagcgta
     2401 tgctgaccaa ctttgctgga cccatgaata actttatttt ggcaatcctg ttattcatca
     2461 ttctggcatt tatgcaaggt ggtgttcaag tgacgaacac taatcgcgta ggagagatca
     2521 tgcctaatgg agctgcggca gaagcaggct taaaagaaaa tgatgaggta gtcagtgtgg
     2581 acggaaaaga aatccattca tggaatgatc tgacaacagt cattacgaaa aatccggata
     2641 aaacattaga ctttaagatc gaacgagaag gtcaggtgca atctgtcgac gtgacaccga
     2701 aatctgtaga gtcaaatggc gaaaaagttg gccaacttgg tataaaagcc ccaatgaata
     2761 caggttttat ggataagatc attggaggga cgcgtcaagc tttcagcggt tcattggaaa
     2821 ttttcaaagc cttaggatca ctatttacag gtttcagttt agataaatta ggcggacctg
     2881 tcatgatgta ccaattgtct tcagaagcag caaatcaagg tgtaacaact gttattagtt
     2941 tgatggcgct tctttcaatg aatctcggaa tcgtcaatct gcttccgatt cctgccttag
     3001 atggcgggaa attagtcttg aatatttttg aagggattcg cgggaaacca ttaagtcaag
     3061 aaaaagaagg aatattgaca ttagctggct ttggattttt aatgttgttg atggtgctag
     3121 ttacatggaa cgatattcaa cgattctttt tctagaaaaa gttgacaaat agggtataat
     3181 aacaaacgat aactattttg aggaaataaa ggagtatata gaatgagaca atcaaaaatg
     3241 ttgattccaa cattacgcga agttccaaat gatgcagaag tattaagcca tcaaatcttg
     3301 ctaagagctg gatatatccg ccaagtatca gcgggtattt attcatatct gccattagca
     3361 aatcgtgtat tggaaaaact gaaacgcatt atgcgggaag aatttgaaaa aatcggagcg
     3421 gtagaaatgc tgatgcctgc catcttgcca gcagaacttt gggaagaatc cgggcgttac
     3481 gaaacatatg gaccaaactt gtatcgtttc aaagaccgca atgatcgaaa aatgatcctt
     3541 ggaccaactc acgaagaaac ttttactgat ttgatgcgta atgaaatcaa ttcatataag
     3601 aaattacctt taaatcttta tcaaatccaa cctaaatatc gtgacgaaaa acgacctcgc
     3661 ttcggtttgc ttcgcggaag agagtttatc atgaaagatg cgtattcttt ccatgcaacg
     3721 gaagaaagtt tggacgagac atataaagat tacgaaaaag cctacaccga agtattcaag
     3781 cgttgtggct tagaatttcg ctcgattatc ggagacggtg gcgcaatggg cgggaaagac
     3841 tcaaaagaat tcatggcgat ctcagaaatc ggggaggata cgatctgtta ttcgactgaa
     3901 agtgattatg ctgcaaactt ggagatggct acaagctact atgtatcaaa aaaatcgcat
     3961 gaaacacagc ttgaattgga gaaagtagct acacctaacg taaaatcaat tgacgaagta
     4021 gcagatttct tttctgttga acctcaaaaa atcatcaagt ctgtattgtt cattgcggat
     4081 gaacaacctg tccttgtttt ggtacgcgga gaccatgaag tcaatgacgt aaaactgaaa
     4141 aactttttat ctgctgactt tttagaagaa gcaacagaag aagaagcaca aaaatatcta
     4201 ggtgctgagt ttggttctgt cggaccagtc ggtgtttcag atgacgtaaa agtatatgca
     4261 gaccgttatg tccaagattt agcgaatgcc attactggag cgaacgaaac aggattccac
     4321 tataaaaatg tgaatccaga acgcgatttc actgtacttt cttatgaaga tcttcgtttt
     4381 gtacaagaag gagatccttc accagacaac aatggtgttc ttgcctttac tcgaggaatc
     4441 gaaatcggac atatcttcaa aatcggcact cgttacagtg aatctatggg agcaactgta
     4501 ctagacgaaa atggacgaga aaaatttgtg attatgggct gttatggtat cggtgtaagt
     4561 cgtttattat cagcaatcgt agaacaacat tcagatgaac aaggaatcaa ctggccaaaa
     4621 ggaattgcgc catttgattt gcatatcgtc caaatgaatc taaaagacga ataccaaaca
     4681 aacctgacga atgaactaga agaagcgatg acaaaagctg gctatgaagt actggttgat
     4741 gacagaaatg aacgcgcagg agtgaaattc gcggattctg atttgattgg ctgtccaatc
     4801 agaatcactg tcggtaaaaa agcagtggat ggaatcgtag aagtgaaaat caagaaaact
     4861 ggcgaaatgg ttgaagtacg taaagaagaa ttggtgaata ctttaccaat cctcttgaac
     4921 caaggcgaat aataagcgtt aaaacacaga aaataaaaat agctgaatca gctgtttttg
     4981 acaatctgat gattcgcttt taaaattgga actgcgattt gaatcgcagt tccaattttt
     5041 taatgtaaaa ctcagaaaaa acgagtatac taaaaggtac tgaaaaagaa gaggataaga
     5101 tgcgatcaat tgcattgttt ctcatcagaa agggcgtata tctttgtcag aaaaacgaga
     5161 tttatttgaa aaactgttgg aacaaatcca actagaggaa gcagaaaaaa atcatccact
     5221 gcttactgct ggtgaaatgg atagtgtagt ggtccatcgt aaatcgcgtt tatgggaatt
     5281 tacgctgcat ttttccaaaa ttttgcctat tatgctatat cggtcattga ctcagcatct
     5341 gactatcgct tttaaagata ttgcacaagt caaactgaat atcttagcag atgaccagac
     5401 attcgatgaa cagctcttgc aagattactg ggcccaagct ttggaaaatc agcaatgcga
     5461 tacaccacta gcacagcaag tattgaaaac gcaggtacct gtgataaaag atcgtaaagt
     5521 catcttgcca atcgacagta caggagctat ttcttatttg aagcaacaat acttgccatt
     5581 agtagaagag ttgttcgtca gttacggatt cccaaaattt cgtatcgaac cagaagtgga
     5641 tgagcagcaa gcggaacgag tattgaaact ttttgaagaa cgcaaacaag aacaggcaga
     5701 agcctttatg aagcaagcag ctgaaagtct agttgtccac gaacaaaaga aaaaggaaag
     5761 aaaagagcaa tcgccagctc ttgaaggacc catccacatc cagctaggac gaaatattcc
     5821 agccgatgaa ccaacgacac caatgatcag tatcgttgaa gaagaacgaa gagtcacact
     5881 tgaggggtac gtattcgaca aagaagtcag agaactacgt tcaaaacgca agatcttgac
     5941 tttgaaaatc acagattata cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa
     6001 agatgaacaa gtatttgatg cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat
     6061 tcaagaagat acgtttgtcc gtgatttggt catgaatgcg caagatatca tagaagtcaa
     6121 acatacacct cgcaaagatt atgctcctga aggggaaaaa cgagtagagc ttcatgttca
     6181 tagcaatatg agtacgatgg atgcaacaaa cagcatctct gatcttgtgg cacaagcagg
     6241 gaaatgggga caccgagcta tcgccattac agaccacggt ggtgcgcaag cctttccgga
     6301 agcacatagt gctggaaaaa aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt
     6361 cgtagatgat ggtgtcccaa ttgcttataa tgatgcacat gaagcattga gtgaagccac
     6421 atatgtcgta tttgacgtgg aaacgacagg attgtctgca gtatatgaca cgatcatcga
     6481 gctagccgca gtcaaaatgt ataaaggaaa tgtgatcgaa agctttgatg aatttatcga
     6541 tccaggacat cctttgtcac gaacaacggt agatctgact ggtatcacgg atggaatggt
     6601 tcgaggatca aagtcggaag aagaagtatt gcggatgttc cttgaatttt caaaagatac
     6661 aattttggta gcccacaacg cggcttttga tatggggttc ttgaatacta gctacgcaag
     6721 gtatggtatt cctgaagcgg ccaatccagt cattgatacg ttggaattag ctagatatct
     6781 gtatcctcaa ttcaaacgtt tcggattagg tgtgttatct aaaaaatttg gtgtcagctt
     6841 ggaacagcat caccgagcaa tctatgatgc tgaagcaact ggtcacttag catggatctt
     6901 tgtgaaagag gcaatggaca atcataacat gctttatcat gatcagctaa atgagcatat
     6961 cggagaagga gattcttaca aaagggctcg acctttccat gtaacgattt tagcaaaaaa
     7021 tcaggcagga ctgaaagatt tgttcaaact gatttcaatg tcgaatgtcg aatacttcga
     7081 acgcgtccca cgaataccac gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg
     7141 ctcagcttgt gataagggag aaatattcga agccatgatg caaaaagggg tagaagaggc
     7201 aagaaaccga gcaaaattct acgattatat tgaagttatg ccaaaagcgg tctatgcgcc
     7261 tttgatcgag caagaattgg taaaaaatga acatgatttg gaagaaatta tccaaaattt
     7321 ggtagaaatc ggaaaaagtt tggataaaat cgttgttgca acaggtaatg tacattatct
     7381 gaacgaagaa gatgccattt accgaaaaat tttgatcaat tctatgggcg gagcgaatcc
     7441 gttgaatcgt catagtttgc cggatgtcca tttccgaaca accgatgaga tgctaactgc
     7501 atttcacttt ttaggggaag aaacagcgaa agagatcgta gtagaaaata cgaataaaat
     7561 cgcagatatc tgcgaagaag tgattccagt caaagatgaa ctttatacac cgaagattcc
     7621 aggttcagaa gatgaaatat cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga
     7681 tccgttacct gagatcattc aaaaacgatt aaaaaaagag ttgaattcta ttaatggtaa
     7741 tggcttctcg gttatttatc tgatcgccca aaaactagtg cataaaagta atgaagatgg
     7801 ctatctagta ggttcacgtg gctctgttgg ctctagcttt gttgcgacga tgacaggaat
     7861 cactgaagtt aatccgttag cccctcatta ttattgtcca gagtgccaat attccgagtt
     7921 ttttgaagat ggtacgtatg gttctggatt tgacatgcca gaaaaacaat gtccaaaatg
     7981 tggtgcacgc ttaaacaaag acggtcatga tattccattt gagacatttt taggattcca
     8041 tggagacaaa gtgcccgata ttgatttgaa cttctcaggt gattatcaag cagaagccca
     8101 taactatacg aaagtattgt ttggcgaaga ctatgtttac cgtgccggaa cgattggtac
     8161 agtcgccgac aaaacagcat atggctatgt aaaaggatac gaaagggaca ataatctgca
     8221 gtttcgaagt gcagaagtcg acagattagc aaaaggcgct actggagtca aacggacaac
     8281 cggccagcat ccagggggga tcatcgttat tcccgattat atggatgtct atgattttac
     8341 gccgatccaa tatccagcag acgatcaaaa ttcggaatgg aaaacgactc attttgattt
     8401 tcactcgatt cacgataatg tattgaaatt agatattcta gggcatgatg acccaactgt
     8461 tattcggatg cttcaagatt tatcagggat cgatccacaa acgatcccta cggatgaccc
     8521 ggaagtgatg cggatatttg ccggtcctga agtacttgga gtaagtcagg aacaaattta
     8581 ttccaaaaca gggacattag gtattccaga attcggcact cgttttgttc gagggatgct
     8641 agaagaaacg catcctacaa cttttgcaga actactgcaa atttccggac tatctcatgg
     8701 tacagacgta tggttaggaa atgcggaaga attgattcga cgaggagatg cgacactggc
     8761 ggaagtaatc ggctgtcgtg atgatattat ggtttatttg atccatgctg gattagatag
     8821 cgggatggca tttaaaatca tggaaactgt tcgtaaagga caatggaaca agatccctga
     8881 tgaattgcga gaaacctatc tatcggcgat gaaagaaaat aatgtgcccg actggtatat
     8941 cgattcttgt tcaaaaatca aatatatgtt tccgaaagcc catgctgcag cttacgtatt
     9001 gatggctctt cgtgtcgctt atttcaaggt ttatttccct attttgtatt actgtgcata
     9061 cttttctgta cgtgcagacg actttgatct agtctccatg tgcaaaggaa aagatgctgt
     9121 gaaacaagca atgaaagaaa tcacggacaa aggattagat gcttccgtaa aagaaaagaa
     9181 tcaactgaca gtgctggagt tagccaatga aatgctggaa cgaggattca aatttggaat
     9241 gattgatttg tataaatcgg atgccgttaa ctttgttatt gaaggtgata cgttgatcgc
     9301 accatttaga gctgtcccaa gtttgggaac caacgtggca aaacaaatcg ttgaagcaag
     9361 aaaagacggt ccatttttat ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac
     9421 attgatcgaa tatatgaacg acaatggcgt attgaaagat ctaccagatg aaaatcaatt
     9481 atcgttattc gatatgctat aagcatctgt tttcctgtct gcttgtaatt ttaagcagag
     9541 tatggtatag ttcttatagg taatgatact aactgtgagt gagcggattt ttcgctcact
     9601 ctttttaatg gaattacgca cttaattttt taaggaggcg aaacttttga gtagcgtcgt
     9661 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9721 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9781 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9841 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9901 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9961 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
    10021 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
    10081 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10141 aaatgagtaa agaaatgtta aatgcgttag acgctttaga agctgaaaaa gggatctcaa
    10201 aagaaatcgt gatcgatgct ttagaagcag cattggtttc tgcatacaaa cgtcactatg
    10261 gccaagcaca aaacgtcgaa gtggaatttg atcagaaaaa aggaaagatc catgtctatg
    10321 cggtaaaaga agtaacggaa gaagtgatgg attcacagtt ggaagtctca ttgaaagatg
    10381 cacttttaat caatccagca tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa
    10441 aagattttgg gcggatcgct gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg
    10501 aagcagaaag aacgattatt tataacgaat tcagtgctta tgaaaaagac atcatgcaag
    10561 gaatcgtcga acgccaggat aaacgttata tttatgtcaa tcttggtaaa attgaggcag
    10621 ttctttctaa acaagatcaa atgccaaatg aattttatca accacatgat cgaatcaaag
    10681 tttatgtttc ccgagtagaa aatacgtcta aaggtccgca agtatttgtt agccgcagcc
    10741 atccagacct attgcgccgc ttatttgagc aagaagtacc agaagtctat gatggaatcg
    10801 ttgaaattgt cagcatcgcc cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa
    10861 atgatccaaa tatcgatgca gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag
    10921 caatcgtcaa tgaattaaaa ggcgaaaaca tggatatcgt cgaatggaat gaagatccag
    10981 ctgtattcat tgcaaatgca ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga
    11041 caaatccaaa ggcctgcaca gttgtcgttc cagactacca attatcgcta gcaatcggta
    11101 aacgcggaca aaacgcacgt ttagctgcta aattgactaa ccataaaatc gatatcaaat
    11161 cagaatctga catgacggaa ttttatgaaa atcaagaaca acaaaaagaa acagaagaac
    11221 tgcatgatga agcaatcgtt cagtcagatt taacagacga tgaatatgaa acaattgcat
    11281 tcaacaatga aacggtcgaa gaaaaaccag aagcttaact ttagtaagga ggcaaagaga
    11341 tgaaacaaag gaaaattcct ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa
    11401 aagagatggt ccgaatcacc cgttcaaaag aaggggaagt atcgatcgat ccaactggaa
    11461 aaatgcctgg acgaggagcg tatgtctcac ttgagccgga agaagtacaa caagcttggg
    11521 ataaaaaaat tctcgatcga gtactcgaaa caacattaac agatgagttt tatcaagaac
    11581 ttcttgacta tgtcactcac caaaaagcac gtaaagagtt atttggaaaa tgaatacagt
    11641 taatcggcaa aaagccatga atctcatcgg tctagcgatg cgcgcgggaa aactaatcac
    11701 gggtgaagaa ctgaccattg ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc
    11761 aaccgatgca agcgagaata caagaaaaaa gataaaagat aagagttcat attatgaagt
    11821 tccttgcttt gagctatttt ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa
    11881 ggtattcgga ataatggatg ccggatttgc gaagaagacc aaggagctaa ttgaaggtta
    11941 ggaaggtgat tgcatgggca acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc
    12001 tagtaaagac gtcgtggaaa aagcacaaca acttggcatc gatgtgaaaa accatatggg
    12061 tgcgattact agcaatgatg agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa
    12121 acaacaagct gcacaacaag caagcagaaa accagccaat caacagccga acgatcaacg
    12181 taaaaaagaa acaacgaaca atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg
    12241 cggtcaaggg agcggtcaag tgaatcaagg aaaaaaacca acaacaaacc aaaataatac
    12301 gggaaatacc agcagccaaa atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac
    12361 taacaataac aacaaaccaa acaataacaa tcgcggcaaa tttaataata ataaccgtaa
    12421 tcgttataat aaaaaaggta aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc
    12481 accacgcaaa ttccgtgaat tgccagatgt attagagtat actgaaggta tgaacgtagc
    12541 agatattgcg aaaaaaattc atcgtgagcc agcagaaatc attaaaaaat tatttatgct
    12601 tggtgtcatg gtcaatcaaa accaagcact ggataaagat acgattgaac tgttagcgac
    12661 ggactatggc atggaaccac aagaaaaagt ccaagttgac attgctgata tcgataaatt
    12721 cttcgaggca gaagaagtcg atccagataa attagtctca cgtccgccag tcgtaacgat
    12781 tatgggacac gtagaccatg ggaaaacaac tttattggat acattacgcc attcacgtgt
    12841 aacaagcggc gaagccggcg gaatcacaca gcatatcggt gcctaccaga tcgatattga
    12901 tggtaaacca atcactttct tggatacacc aggacatgcg gcctttacaa gtatgcgtgc
    12961 acgtggagca agtatcacag atatcacgat cttagttgtt gctgcagatg atggcgtcat
    13021 gccacaaaca gttgaagcaa tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc
    13081 tgtaaataaa attgataaac caggtgcaaa ccctcaacat gttatgcaag aattaagtga
    13141 gtatgagtta attcctgaag catggggtgg agaaacgatc tttgttgaga tctcagcgaa
    13201 attcggccaa aacatcgaag aattgctgga aatgatcctg ttagtcgcag aagtagaaga
    13261 tttaaaagca gatccaacac aacgcgcaat cggaacagtg atcgaagcac gtcttgataa
    13321 aggtaaaggt cctgtgtcaa ccttacttgt ccaagaagga acacttcatg ttggtgaccc
    13381 aatcgttgtc ggtaatactt acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg
    13441 cgataaagaa gcaggacctg caacgccagt agaaatcact ggcttgaatg atgtacctca
    13501 agcaggtgac cgtttcgtag tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga
    13561 acgcgcgaaa cgtgcattat tagaaaaccg tgctgccaca agccgtgtga ctttggataa
    13621 cttatttgaa agcctgaaag aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga
    13681 tgtacaaggt tcagcagaag cactggctgc atcattgaag aagattgatg tggaaggtgt
    13741 ccgtgtaaaa atcgtccatt cagctgtcgg agcaatcaat gaaagtgatg taaccttagc
    13801 agcagccagc aacgctatta tcatcggttt caacgttcgt cctacaccgc aggcaaaagt
    13861 gcaagctgat actgaagaag tcgatattcg tcttcaccgt atcatctata aagccatcga
    13921 agaaatcgaa actgcgatga aagggatgct agaccctgaa tttgaagaaa aaatcaccgg
    13981 tcaaatgatc gttcgtgaaa ccttcaaagt ttctaaagtt ggtacgattg caggtgccta
    14041 tgtaacagaa ggatatattc gtagagatag cggtgtccgt gtaatccgtg acggaatcgt
    14101 aatctatgaa ggccaacttg caagtctgaa acgattcaaa gatgacgtga aagaagtcaa
    14161 aatgggttat gaatgtggtg caatgattga aaaattcaat gacatcaaag tagatgacgt
    14221 aattgaagga tttatcatgg aagaagtcaa aaatgactaa aaactgataa aaggaggcca
    14281 atcattatgg ctaactaccg tgaccgccga gtcggtcaag aaatcttaaa agaagtcaat
    14341 gatattttgc gcaaaaaggt ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg
    14401 aaagttacgg gggacttaca gcaagcaacg atctattaca gcattttatc tgacttagca
    14461 tcagataaga aaacagcaca agatggattg aataaagcat caggattgat tcgtcgagag
    14521 ttagggcaca atctaagtat ttataagaca cctgaattgt tctttgaact tgatgaatca
    14581 gttgtctatg gcaaccatat cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa
    14641 actcccgcaa atcctgtgaa atcaggggtt gcgggatttt tttgttttca ttaagacgta
    14701 ttacctaaga gttccatgac tttagtcttg cggcgtgtca gctaaccaaa aaagagaaaa
    14761 agaaaagtaa aaaattcgac tttctttttt ctagtagtat ataaattttt gcatatagat
    14821 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14881 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14941 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    15001 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    15061 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15121 ggaattgctc gatccagccc cagttttcca gcaatgtgtt aactggccca gcgtcattca
    15181 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15241 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15301 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15361 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15421 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15481 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15541 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15601 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15661 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15721 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15781 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15841 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15901 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15961 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    16021 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    16081 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16141 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16201 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16261 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16321 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16381 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16441 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16501 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16561 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16621 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16681 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16741 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16801 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16861 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16921 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16981 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    17041 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    17101 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17161 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17221 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17281 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17341 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17401 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17461 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17521 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17581 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17641 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17701 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17761 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17821 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17881 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17941 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    18001 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    18061 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18121 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18181 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18241 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18301 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18361 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18421 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18481 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18541 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18601 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18661 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18721 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18781 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18841 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18901 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18961 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    19021 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    19081 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19141 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19201 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19261 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19321 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19381 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19441 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19501 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19561 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19621 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19681 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19741 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19801 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19861 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19921 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19981 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    20041 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    20101 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20161 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20221 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20281 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20341 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20401 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20461 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20521 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20581 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20641 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20701 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20761 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20821 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20881 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20941 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    21001 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    21061 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21121 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21181 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21241 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21301 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21361 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21421 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21481 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21541 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21601 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21661 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21721 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21781 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21841 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21901 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt
    21961 atcggattat tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga
    22021 tggtaaaatt tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa
    22081 aaaatagcgc tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga
    22141 tacaagggaa atatctgtct ggatcaataa tttttcattg atccattgac taaagtttac
    22201 gacataattg aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta
    22261 ttgtaaaaga cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc
    22321 tcggaattga ttgattgctg taaaatcacg atattgaagg ttgaaacgca tcgtgattgg
    22381 tgcatttagc atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag
    22441 gaactcaaaa agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag
    22501 ttttccttca tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa
    22561 ttcatttact gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac
    22621 ttagttgata tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt
    22681 aaaaatcaag tcagaaaatt ctaactttta tttctctaaa gaaattcgtt ctctcctaaa
    22741 gtgatattat atttaaatat acctgaaaat aatgggcagt attatgaaag atcactgata
    22801 ttttctcgta ttttttcctt attcaaaagt tttgcaggaa aattgctgaa aaaataattt
    22861 tactatgtag aaaattcaaa gccattcaat cagtgatttt tttcagcaca agaaagactc
    22921 cttataaaaa tagcagatac tttgtcaatt aagtgtttaa ccagggatga taaaaaggat
    22981 aggattgaac aaaacctgta taaggtgatg aatagtttcc tcgccagtaa aaggtttatt
    23041 cgtataaagc tttatcgcca aaatttatca tgaccttttt tatcttcaat attaaatgaa
    23101 atcaattgtt caatcaattc ccagttgatt acacttttcc aaggaacttt gatgacgtta
    23161 tccgtatata cgtagcctgc ttcttggata tttttttcaa gggcacgaat cgctgctttt
    23221 tccggtgcga tagaaaaatg atttttagca aaactaaatg caataataaa ggtaccatgg
    23281 tcagtaaaca taggttggtt ccattttaca gttgtgtcta gctggggata cgtttctgtt
    23341 atcttagcaa aaaggtcttt gatcttttgt tgatgttctt tttctttgat ttgattgatg
    23401 atcggctgaa atttttccat tctaaaacgc ctccattaag tggtttgatt tattttacca
    23461 gaaaaacaat agttatgtat aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata
    23521 tttatatgat aagatgtctc aaataataaa atagaaaaga gacataaaaa tgattgactt
    23581 ggcaaagaaa ttcttacacg aaaatatata ttttgagttg tatcatcatc gagccattta
    23641 tacaaatgaa gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa
    23701 aagcttattc ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa
    23761 acaaacggat ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc
    23821 agaacgcatg gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga
    23881 aaaacagatt ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc
    23941 accaggtaga ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca
    24001 gctacttgag atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa
    24061 aatagtgcat acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc
    24121 aacgattggg gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga
    24181 atcagcttta aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg
    24241 gacgcaagat aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat
    24301 gatgcaagat gaaaatatcc aaatgatctt tccgacttgg ggaggagaat tattgattga
    24361 gattctcgat tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga
    24421 tattagtacg ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac
    24481 taaccttatt gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt
    24541 actgaaaaca agagaaggag aatcgattct acagcaatct tctgaaaagt atcaaaaagt
    24601 gtggccccat gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca
    24661 atcagtcagc ggagatgcag taaggatgaa gggacgatta ttaggtggtt gtattgatac
    24721 gatcaggcat ttaatcggta ctccttatgg agatgtccaa tcttttaaaa gaaagtataa
    24781 tgatggaaag ccgatcttgt ggtttctaga aaactgtgag ctatcgtcag ttgatttaag
    24841 aagatcactt gttcagatga aactggcagg ctggtttgat ggcagctcgg ggatcatgtt
    24901 cggtagaagt gcggcagagg atttgttaga ttataaaatg aaggatgtat acgaagatct
    24961 gtcaaaggaa ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca
    25021 actgacattg attaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt
    25081 tttgcaaacg tttatttaaa aaattttttc ctgccttgga gtctatcaaa gaccaataaa
    25141 tctaaataga aaagaaagtg aaagaagctg acaaaatgct gtcagcttct ttttgtataa
    25201 catattagaa agtctaatta tctataactg tcacatcgat taactgtttc acgtcattgg
    25261 gatttcctgt aggaatagct actacagatg cattatcaat gatttcttga gttgtttctc
    25321 taatgaacgc tggataagat gtttgatctg caggcatttg ccaatgtgaa ggaactggat
    25381 aactagtgat tgtcccgtca tggttacttg aatacactaa aactccctgg taactgtatt
    25441 tcccagtgag cataaccaca tctttcggat agactgcact ccctgcatca taaggggcga
    25501 taggtgttcc tgcaggataa tgaataacat gtaattcgaa atcacccgat gtatctctaa
    25561 aagtattacc tctaatagct agccaaacac gtgcgtactc aatttgtaga tcagaatatc
    25621 ctgctaacgg atctattgac tgagaagact gtgaggaggt tgtgtctatt gttgaagaag
    25681 aaatatttgt ctcaactgta ctactagatg tagaagaagt attgggctct gagactttac
    25741 tgcttgttga agatgtattc gtttcagtag aagctgattt ctgagtactt gatttttcag
    25801 ctgaactggt ttgtctgatt gaacttttgt ctgtggtcgc agatggatca ctattttctt
    25861 tactaggaga cgtacatcct acaaggaata aagagaccaa caataaaata aaacctaact
    25921 ttttcatata aaatacctca ttattttttt aataccagta tagcataatc ctgaattttt
    25981 gaatccgtaa aaccttgtct atgaat
//
LOCUS       DANJLF010000034        25919 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000034 DANJLF010000000
VERSION     DANJLF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="PWT25_001564"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="PWT25_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL1021653.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="PWT25_001565"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="PWT25_001565"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL1021654.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="PWT25_001566"
     CDS             1947..2645
                     /locus_tag="PWT25_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL1021655.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="PWT25_001567"
     CDS             complement(2813..5452)
                     /locus_tag="PWT25_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1021656.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6071..7102)
                     /gene="queA"
                     /locus_tag="PWT25_001568"
     CDS             complement(6071..7102)
                     /gene="queA"
                     /locus_tag="PWT25_001568"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL1021657.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7295..8470
                     /locus_tag="PWT25_001569"
     CDS             7295..8470
                     /locus_tag="PWT25_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1021658.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8474..9115
                     /locus_tag="PWT25_001570"
     CDS             8474..9115
                     /locus_tag="PWT25_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021659.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9112..10029
                     /locus_tag="PWT25_001571"
     CDS             9112..10029
                     /locus_tag="PWT25_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1021660.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10026..10697
                     /locus_tag="PWT25_001572"
     CDS             10026..10697
                     /locus_tag="PWT25_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021661.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11238..11726)
                     /locus_tag="PWT25_001573"
     CDS             complement(11238..11726)
                     /locus_tag="PWT25_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL1021662.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11927..12193)
                     /locus_tag="PWT25_001574"
     CDS             complement(11927..12193)
                     /locus_tag="PWT25_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL1021663.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12193..12456)
                     /locus_tag="PWT25_001575"
     CDS             complement(12193..12456)
                     /locus_tag="PWT25_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1021664.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12932..13117
                     /locus_tag="PWT25_001576"
     CDS             12932..13117
                     /locus_tag="PWT25_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1021665.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13259..15082)
                     /locus_tag="PWT25_001577"
     CDS             complement(13259..15082)
                     /locus_tag="PWT25_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL1021666.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15680..18385)
                     /locus_tag="PWT25_001578"
     CDS             complement(15680..18385)
                     /locus_tag="PWT25_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL1021667.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18691..19638)
                     /locus_tag="PWT25_001579"
     CDS             complement(18691..19638)
                     /locus_tag="PWT25_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL1021668.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19828..20097
                     /gene="rpsN"
                     /locus_tag="PWT25_001580"
     CDS             19828..20097
                     /gene="rpsN"
                     /locus_tag="PWT25_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL1021669.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20273..20635)
                     /locus_tag="PWT25_001581"
     CDS             complement(20273..20635)
                     /locus_tag="PWT25_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1021670.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20646..21767)
                     /locus_tag="PWT25_001582"
     CDS             complement(20646..21767)
                     /locus_tag="PWT25_001582"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL1021671.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21783..22133)
                     /locus_tag="PWT25_001583"
     CDS             complement(21783..22133)
                     /locus_tag="PWT25_001583"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL1021672.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22263..23774)
                     /locus_tag="PWT25_001584"
     CDS             complement(22263..23774)
                     /locus_tag="PWT25_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1021673.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24096..25448)
                     /locus_tag="PWT25_001585"
     CDS             complement(24096..25448)
                     /locus_tag="PWT25_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL1021674.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25601..>25919)
                     /locus_tag="PWT25_001586"
     CDS             complement(25601..>25919)
                     /locus_tag="PWT25_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL1021675.1"
                     /translation="YINNKIEMQIPAEVPEEVYQKAQEYAKLAYTMLGGSGLSRCDFF
                     LTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGDLIEELIQLGMNRYHQRQSFFE
                     KNE"
BASE COUNT         7185 a         5545 c         4853 g         8336 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct
     6061 tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt
     6121 caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca
     6181 tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt
     6241 catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa
     6301 atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc
     6361 cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt
     6421 cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc
     6481 ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt
     6541 ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga
     6601 cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt
     6661 ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc
     6721 cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc
     6781 ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg
     6841 tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac
     6901 gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa
     6961 tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac
     7021 gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat
     7081 caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta
     7141 tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct
     7201 aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata
     7261 gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa
     7321 aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga
     7381 gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa
     7441 ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat
     7501 tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc
     7561 tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga
     7621 agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt
     7681 agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc
     7741 ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat
     7801 cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat
     7861 cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat
     7921 gagtgaaggc cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa
     7981 cgatttcgta gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac
     8041 aagagtcggc gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca
     8101 agaagcaatc aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc
     8161 gaatgtatta aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc
     8221 cagtgtcggc gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg
     8281 tgataccttg caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca
     8341 gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat
     8401 ttggggagaa gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa
     8461 ggaggtctga cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg
     8521 ttttgaacat ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc
     8581 gcttggtgtc ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag
     8641 tgccttgcag acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt
     8701 aggtaaatta cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa
     8761 tacgtacatt gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg
     8821 gatgactgag ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat
     8881 ggctggtatt cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat
     8941 cggcgctggt ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct
     9001 cattttggca ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg
     9061 tctcttggaa caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt
     9121 atactaaaaa tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct
     9181 ggtcttgcca gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa
     9241 gcacagatct tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc
     9301 gcaatcatca acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat
     9361 gcttcaattt cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct
     9421 cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac
     9481 ggtcaaacgt ggttcccgtc ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa
     9541 gacaccgcac aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa
     9601 ttagtagctg gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc
     9661 caagaaactt atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat
     9721 gacgcagtcg aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc
     9781 gccagttatg atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct
     9841 gctccagttg tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat
     9901 aagttaggtc ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat
     9961 aatctagtgg aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa
    10021 gggaagtgaa gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg
    10081 aaaatggctc ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg
    10141 tcctctttgc agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat
    10201 tagcaaactg ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga
    10261 tctccatttt aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt
    10321 tgtattccct cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa
    10381 acatccttga tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag
    10441 agttaccact ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca
    10501 tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc
    10561 gaggaaccaa tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat
    10621 taatggcgat catcacggac tggcttttag gtattttgga aagacggcta gatccagcca
    10681 gccgtacatc acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc
    10741 gcaaaggctt ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc
    10801 ttatgggtaa agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata
    10861 ctgccaacgg gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat
    10921 tttgccaatc cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac
    10981 tcgtctgctt gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt
    11041 taagcctttt ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt
    11101 gactgccgta tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc
    11161 cttggctaaa aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta
    11221 aaaccggtac acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga
    11281 tccttcacca gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag
    11341 acattggcgt aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg
    11401 gccgaaagcg ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc
    11461 gctttggata cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct
    11521 tttccaaaag accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg
    11581 tccattgctt ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc
    11641 ttcgcctcac tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct
    11701 ccaattttac tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa
    11761 cattccattc ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa
    11821 agaaatcatg caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt
    11881 ggggagactc tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg
    11941 tccaggcaga ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat
    12001 gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag
    12061 actgcgtggg catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct
    12121 ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg
    12181 tatagttact catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc
    12241 gtctttttcg cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc
    12301 ttgaatcgct ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact
    12361 aataatctcg atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt
    12421 acgagcagtg ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat
    12481 aattatagcg tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca
    12541 aacttttcct cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac
    12601 gctcacttct tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg
    12661 tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca
    12721 cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac
    12781 gaaagatttg taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa
    12841 aaatgattgc ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta
    12901 gaccgttggg tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg
    12961 gtcacttgac gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt
    13021 aaaggaagaa gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta
    13081 tgatcaagac atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca
    13141 tacgctgttc ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa
    13201 cagaccctct agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt
    13261 atttcttcaa atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca
    13321 atcttaaccg caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa
    13381 attgaggaat caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat
    13441 ctacataacg gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac
    13501 ggacatctgg gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt
    13561 cgtctagcga aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg
    13621 caccgatatt tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat
    13681 catgtagttt tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat
    13741 ggatcttata aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc
    13801 ctaaccgata gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat
    13861 tggcaatcga tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg
    13921 tttgagataa agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg
    13981 ttgagccatg gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat
    14041 tggaatagcc tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg
    14101 ccagattgta tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa
    14161 tcagcgctgt agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac
    14221 caatttgaga caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa
    14281 atgtgatccc cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg
    14341 cattttttgc tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag
    14401 ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca
    14461 cagccgctcc tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata
    14521 aaccaacaac gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga
    14581 acgatgcaga ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca
    14641 cagaaatagc gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac
    14701 ctgctgagac agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa
    14761 tcaagccagc attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag
    14821 gataagcatg gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa
    14881 aagcggcaat gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa
    14941 cgatttgttc cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag
    15001 cagcaaatcg ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta
    15061 acaacctttt taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt
    15121 tatagtcgaa aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca
    15181 ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc
    15241 tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa
    15301 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
    15361 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
    15421 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa
    15481 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
    15541 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    15601 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
    15661 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
    15721 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
    15781 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
    15841 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
    15901 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
    15961 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
    16021 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
    16081 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
    16141 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
    16201 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
    16261 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
    16321 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
    16381 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
    16441 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
    16501 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
    16561 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
    16621 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
    16681 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
    16741 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
    16801 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
    16861 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
    16921 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
    16981 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
    17041 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
    17101 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
    17161 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
    17221 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
    17281 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
    17341 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
    17401 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
    17461 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
    17521 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
    17581 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
    17641 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
    17701 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
    17761 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
    17821 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
    17881 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
    17941 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
    18001 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
    18061 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
    18121 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
    18181 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
    18241 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
    18301 tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
    18361 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
    18421 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
    18481 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taaggattac
    18541 agcctttact ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta
    18601 ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc
    18661 agcttttttc gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc
    18721 agcaggagga tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga
    18781 cagattcatt gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc
    18841 aaaggcttct gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt
    18901 tttttgccgc aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt
    18961 catgatctgg atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc
    19021 tgcctgatca gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc
    19081 tcctaatttt tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc
    19141 cttttcatgg agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat
    19201 tacagtatct gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc
    19261 tggcgtgacc attccggctg cttcttccca actcatttta tctggaattt ttgctacttt
    19321 tgctgatggc aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg
    19381 atcacctaca tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc
    19441 attacctggt acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa
    19501 ggcaacatca taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc
    19561 gacttctctg ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt
    19621 aattgcaaat cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt
    19681 tattttgaca aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc
    19741 ttttttcgcg taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta
    19801 ataaaaaata attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa
    19861 aaacagcaag aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa
    19921 gattacgaag gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt
    19981 gatttaatcg atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa
    20041 ttccgtgaat tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc
    20101 aagtctggct gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa
    20161 gatacgttcg cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac
    20221 gcacagctgg tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag
    20281 taacaggagt cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca
    20341 taatgggtcg atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga
    20401 tctgctccaa caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa
    20461 tgtgagtcgg aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg
    20521 ggctaaaatg gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct
    20581 ccgatcccac gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat
    20641 cctccttatt cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag
    20701 tggatcgtac ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg
    20761 ccgataagcg tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga
    20821 tccatacata cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc
    20881 tgcatctttc gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct
    20941 tcaggagtga tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact
    21001 tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg
    21061 tgcccagagg gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt
    21121 tcatcatgcc ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga
    21181 agttttttca gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc
    21241 tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg
    21301 atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca
    21361 gtgtctgctt ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca
    21421 gctgctgctt gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct
    21481 acagtcagtg cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct
    21541 tctctcaact cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca
    21601 actgctgcct cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca
    21661 aacaattctt ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct
    21721 tttttatgta tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct
    21781 ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc
    21841 catacattgc cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa
    21901 atctcgacat cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc
    21961 tctttgcaag cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg
    22021 aatagtttca tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc
    22081 agccgagtga tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt
    22141 tctcttttca ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa
    22201 ataagtccac aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat
    22261 gtttaatcgt tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg
    22321 ccatcttttt ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa
    22381 ttaccgccgt cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat
    22441 ccttttttct gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct
    22501 ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct
    22561 aataatttat ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg
    22621 atttgttcaa ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt
    22681 aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt
    22741 ggtgtaacga aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg
    22801 tgaacgtagc tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa
    22861 atatctaatc cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta
    22921 aatgaacgca atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca
    22981 cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg
    23041 aagacgattg ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt
    23101 tcgtattctt ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct
    23161 ttgatcttca catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc
    23221 ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt
    23281 tcgatatctt ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt
    23341 tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta
    23401 cccacaacga tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg
    23461 ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt
    23521 gtttggatag ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct
    23581 ggatcgattt tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt
    23641 gcttgtccaa taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc
    23701 gttgcttctt caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt
    23761 tctttaaatt tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc
    23821 taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt
    23881 ttttatccat tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat
    23941 tagaaaagcc aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa
    24001 gttgtttcta tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta
    24061 acatagtttt tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct
    24121 accacttcaa ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca
    24181 agattttgtt tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt
    24241 agttgggggt atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg
    24301 aaaatcatgg cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa
    24361 gactctttcc ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc
    24421 agatttttaa aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg
    24481 ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa
    24541 acatgggcga gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta
    24601 tacgcaatca aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct
    24661 ccttctacta caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg
    24721 ccatttccaa taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc
    24781 aacggttcat ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc
    24841 attttggctt tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc
    24901 attgtgattg ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg
    24961 tgatccatgc ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg
    25021 taaggaagac caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca
    25081 gacaagacag acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca
    25141 acgacttctg cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa
    25201 ggatctgcta caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa
    25261 aatgaagcag atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta
    25321 ccagcaagag gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg
    25381 gtaatctttg tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt
    25441 agattcattg agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg
    25501 gttcgcatga ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa
    25561 cgcggttacg caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa
    25621 aagattgacg ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac
    25681 cgtatttcaa gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg
    25741 gcatagagtt taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc
    25801 ggctcaatcc gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt
    25861 gataaacttc ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatat
//
LOCUS       DANJLF010000035        23832 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000035 DANJLF010000000
VERSION     DANJLF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(56..790)
                     /locus_tag="PWT25_001587"
     CDS             complement(56..790)
                     /locus_tag="PWT25_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL1021676.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="PWT25_001588"
     CDS             1117..1527
                     /locus_tag="PWT25_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL1021677.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="PWT25_001589"
     CDS             1520..2221
                     /locus_tag="PWT25_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL1021678.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVELGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="PWT25_001590"
     CDS             2221..3807
                     /locus_tag="PWT25_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021679.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="PWT25_001591"
     CDS             3826..4047
                     /locus_tag="PWT25_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL1021680.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="PWT25_001592"
     CDS             4044..4805
                     /locus_tag="PWT25_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL1021681.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="PWT25_001593"
     CDS             5185..5694
                     /locus_tag="PWT25_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL1021682.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="PWT25_001594"
     CDS             complement(5769..6308)
                     /locus_tag="PWT25_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL1021683.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="PWT25_001595"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="PWT25_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL1021684.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="PWT25_001596"
     CDS             7321..8172
                     /locus_tag="PWT25_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL1021685.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="PWT25_001597"
     CDS             8200..8835
                     /locus_tag="PWT25_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL1021686.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="PWT25_001598"
     CDS             8855..9628
                     /locus_tag="PWT25_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1021687.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="PWT25_001599"
     CDS             10036..10833
                     /locus_tag="PWT25_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL1021688.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="PWT25_001600"
     CDS             10811..11224
                     /locus_tag="PWT25_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL1021689.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="PWT25_001601"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="PWT25_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL1021690.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="PWT25_001602"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="PWT25_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL1021691.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /gene="maf"
                     /locus_tag="PWT25_001603"
     CDS             16001..16561
                     /gene="maf"
                     /locus_tag="PWT25_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL1021692.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="PWT25_001604"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="PWT25_001604"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL1021693.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="PWT25_001605"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="PWT25_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL1021694.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="PWT25_001606"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="PWT25_001606"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL1021695.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="PWT25_001607"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="PWT25_001607"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL1021696.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="PWT25_001608"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="PWT25_001608"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL1021697.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="PWT25_001609"
     CDS             21908..22486
                     /locus_tag="PWT25_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1021698.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="PWT25_001610"
     CDS             22538..23020
                     /locus_tag="PWT25_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL1021699.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="PWT25_001611"
     CDS             23137..23511
                     /locus_tag="PWT25_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021700.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7785 a         4284 c         5115 g         6648 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggagtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagcggct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcacccg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatagaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa taatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcatagc atgacagcat
    23641 tcggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcattagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tt
//
LOCUS       DANJLF010000036        23261 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000036 DANJLF010000000
VERSION     DANJLF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWT25_001612"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWT25_001612"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL1021701.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="PWT25_001613"
     CDS             complement(1172..1867)
                     /locus_tag="PWT25_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL1021702.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="PWT25_001614"
     CDS             complement(1880..2596)
                     /locus_tag="PWT25_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL1021703.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="PWT25_001615"
     CDS             2926..3534
                     /locus_tag="PWT25_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL1021704.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="PWT25_001616"
     CDS             3859..4182
                     /locus_tag="PWT25_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL1021705.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="PWT25_001617"
     CDS             4364..5734
                     /locus_tag="PWT25_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL1021706.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="PWT25_001618"
     CDS             complement(5864..7165)
                     /locus_tag="PWT25_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL1021707.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="PWT25_001619"
     CDS             complement(7519..9477)
                     /locus_tag="PWT25_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL1021708.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="PWT25_001620"
     CDS             complement(9654..9920)
                     /locus_tag="PWT25_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1021709.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="PWT25_001621"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="PWT25_001621"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL1021710.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="PWT25_001622"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="PWT25_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL1021711.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="PWT25_001623"
     CDS             13264..13827
                     /locus_tag="PWT25_001623"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL1021712.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="PWT25_001624"
     CDS             complement(14080..14601)
                     /locus_tag="PWT25_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL1021713.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="PWT25_001625"
     CDS             complement(14604..15446)
                     /locus_tag="PWT25_001625"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL1021714.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="PWT25_001626"
     CDS             complement(15439..16164)
                     /locus_tag="PWT25_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL1021715.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="PWT25_001627"
     CDS             16340..16636
                     /locus_tag="PWT25_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021716.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="PWT25_001628"
     CDS             17204..18796
                     /locus_tag="PWT25_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1021717.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="PWT25_001629"
     CDS             18809..19549
                     /locus_tag="PWT25_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1021718.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="PWT25_001630"
     CDS             19717..21066
                     /locus_tag="PWT25_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1021719.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="PWT25_001631"
     CDS             complement(21194..21784)
                     /locus_tag="PWT25_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL1021720.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="PWT25_001632"
     CDS             22020..23099
                     /locus_tag="PWT25_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1021721.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         6661 a         4820 c         4527 g         7253 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat g
//
LOCUS       DANJLF010000037        23064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000037 DANJLF010000000
VERSION     DANJLF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(110..565)
                     /locus_tag="PWT25_001633"
     CDS             complement(110..565)
                     /locus_tag="PWT25_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021722.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="PWT25_001634"
     CDS             complement(719..1927)
                     /locus_tag="PWT25_001634"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL1021723.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="PWT25_001635"
     CDS             complement(2039..2371)
                     /locus_tag="PWT25_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL1021724.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="PWT25_001636"
     CDS             2519..3394
                     /locus_tag="PWT25_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1021725.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="PWT25_001637"
     CDS             complement(3476..4057)
                     /locus_tag="PWT25_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL1021726.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="PWT25_001638"
     CDS             complement(4063..5322)
                     /locus_tag="PWT25_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL1021727.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="PWT25_001639"
     CDS             complement(5324..6370)
                     /locus_tag="PWT25_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021728.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="PWT25_001640"
     CDS             complement(6605..6880)
                     /locus_tag="PWT25_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL1021729.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="PWT25_001641"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="PWT25_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL1021730.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="PWT25_001642"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="PWT25_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL1021731.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="PWT25_001643"
     CDS             complement(9979..10659)
                     /locus_tag="PWT25_001643"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL1021732.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="PWT25_001644"
     CDS             complement(10708..11343)
                     /locus_tag="PWT25_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1021733.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="PWT25_001645"
     CDS             complement(11409..12812)
                     /locus_tag="PWT25_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL1021734.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="PWT25_001646"
     CDS             complement(12799..13830)
                     /locus_tag="PWT25_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021735.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="PWT25_001647"
     CDS             13861..14082
                     /locus_tag="PWT25_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL1021736.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="PWT25_001648"
     CDS             complement(14247..14984)
                     /locus_tag="PWT25_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL1021737.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="PWT25_001649"
     CDS             complement(15137..16477)
                     /locus_tag="PWT25_001649"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL1021738.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="PWT25_001650"
     CDS             complement(16714..18237)
                     /locus_tag="PWT25_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL1021739.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="PWT25_001651"
     CDS             complement(18615..19226)
                     /locus_tag="PWT25_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1021740.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="PWT25_001652"
     CDS             complement(19556..20272)
                     /locus_tag="PWT25_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL1021741.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="PWT25_001653"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="PWT25_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL1021742.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21628)
                     /locus_tag="PWT25_001654"
     CDS             complement(20831..21628)
                     /locus_tag="PWT25_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL1021743.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="PWT25_001655"
     CDS             complement(21598..21969)
                     /locus_tag="PWT25_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL1021744.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="PWT25_001656"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="PWT25_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL1021745.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagc
    23041 cccattttca ttttaggtat taaa
//
LOCUS       DANJLF010000038        22457 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000038 DANJLF010000000
VERSION     DANJLF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..152
                     /locus_tag="PWT25_001657"
     CDS             <1..152
                     /locus_tag="PWT25_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1021746.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="PWT25_001658"
     CDS             complement(230..763)
                     /locus_tag="PWT25_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL1021747.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="PWT25_001659"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="PWT25_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL1021748.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="PWT25_001660"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="PWT25_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL1021749.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="PWT25_001661"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="PWT25_001661"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL1021750.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="PWT25_001662"
     CDS             complement(4488..5066)
                     /locus_tag="PWT25_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL1021751.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="PWT25_001663"
     CDS             5388..5858
                     /locus_tag="PWT25_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL1021752.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="PWT25_001664"
     CDS             complement(5966..7192)
                     /locus_tag="PWT25_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL1021753.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="PWT25_001665"
     CDS             complement(7946..8146)
                     /locus_tag="PWT25_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1021754.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="PWT25_001666"
     CDS             complement(8280..8888)
                     /locus_tag="PWT25_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL1021755.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="PWT25_001667"
     CDS             complement(8973..9443)
                     /locus_tag="PWT25_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL1021756.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="PWT25_001668"
     CDS             complement(9491..10405)
                     /locus_tag="PWT25_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL1021757.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="PWT25_001669"
     CDS             10551..11354
                     /locus_tag="PWT25_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL1021758.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="PWT25_001670"
     CDS             11532..12479
                     /locus_tag="PWT25_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL1021759.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="PWT25_001671"
     CDS             complement(12547..12840)
                     /locus_tag="PWT25_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL1021760.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="PWT25_001672"
     CDS             complement(12868..13533)
                     /locus_tag="PWT25_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL1021761.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="PWT25_001673"
     CDS             complement(13673..14305)
                     /locus_tag="PWT25_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1021762.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="PWT25_001674"
     CDS             complement(14312..15379)
                     /locus_tag="PWT25_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1021763.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="PWT25_001675"
     CDS             complement(15376..16107)
                     /locus_tag="PWT25_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL1021764.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="PWT25_001676"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="PWT25_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL1021765.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="PWT25_001677"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="PWT25_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL1021766.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="PWT25_001678"
     CDS             18270..19439
                     /locus_tag="PWT25_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL1021767.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..21340)
                     /locus_tag="PWT25_001679"
     CDS             complement(19706..21340)
                     /locus_tag="PWT25_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021768.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            21825..22457
                     /locus_tag="PWT25_001680"
                     /pseudo
     CDS             21825..22457
                     /locus_tag="PWT25_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         6971 a         4369 c         3556 g         7561 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt
    21301 ttacagaaaa aatttttttc aaattcatat accacctcat gtttttttat attacatata
    21361 gtatcattta tttttttaaa acactagatt actcactata taaaatgtga ttaattttat
    21421 tattaataaa atatttttta tattacaaaa gtagtgtttc aaagttagta gagaaaatgt
    21481 agttatatat ttaaattttt accagaaaga aatgaacacc ttcaattatc taaatctcga
    21541 aaatggttag atgtaatcat caggaaaaac aatcttaaac gaattactat tcatggtctt
    21601 agacacacac atgctagctt gcttttagca agctagtgca aatattaaag atgtgcaaga
    21661 gcgtttaggc cactcttcta ttcaaatcat tatggatcta tatattcata ttacagacaa
    21721 aagaaaagaa gaaaccgtag cgcaattcgc aaaatatagg ctctataatt tatgggactg
    21781 atgaatcaaa attgattctt cagtcccatt gttattttag gtatatgatt cgtacattga
    21841 aagagttgaa aaactatctt ccgaagccgt caaaaaggac cctgccaaaa gtattgatgg
    21901 tggatgaatt tcgttcgcac gcttcaattg tggataaaat gagctttatt tgtgcggatg
    21961 gcgagactgg aaaactcatt gatattttgc ctacacgaaa attgtctcga ttaacacact
    22021 attttagtcg ctgtgcaaat cctgaagcgg tggaatattt agtgacagat atgaacgccg
    22081 cctacttcca actgacaaaa cgtgtcttac caaatgcgaa agtcgtcatt gattgttttc
    22141 atatcatcaa acatttgaat caggcgttca atgattttcg tgtacaagaa atgaaaagat
    22201 tacaccagtt agggcaacga cgggaagccg agaaagtgaa aaagaactgg cgctttcttc
    22261 taaaaaaccg tcaaaaaatc aatcactatg attacaaaac atggcgcagt tttcgtgcac
    22321 ctaaataccc gctactcact gaagcaatga tgattgatcg attgctggca ttttcaacgc
    22381 ctttaaagga cgcctatact tttttccatg agctgaccga agcctttaga gacaaggaat
    22441 cagacttatt ttttacc
//
LOCUS       DANJLF010000039        22031 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000039 DANJLF010000000
VERSION     DANJLF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            367..555
                     /locus_tag="PWT25_001681"
     CDS             367..555
                     /locus_tag="PWT25_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021769.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="PWT25_001682"
     CDS             545..805
                     /locus_tag="PWT25_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021770.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="PWT25_001683"
     CDS             846..1073
                     /locus_tag="PWT25_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021771.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="PWT25_001684"
     CDS             1138..1710
                     /locus_tag="PWT25_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021772.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="PWT25_001685"
     CDS             1711..2022
                     /locus_tag="PWT25_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL1021773.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="PWT25_001686"
     CDS             2013..2273
                     /locus_tag="PWT25_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021774.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="PWT25_001687"
     CDS             2434..2793
                     /locus_tag="PWT25_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021775.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="PWT25_001688"
     CDS             2790..4433
                     /locus_tag="PWT25_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL1021776.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="PWT25_001689"
     CDS             4468..5550
                     /locus_tag="PWT25_001689"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021777.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="PWT25_001690"
     CDS             5594..6766
                     /locus_tag="PWT25_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL1021778.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="PWT25_001691"
     CDS             6753..7457
                     /locus_tag="PWT25_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL1021779.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="PWT25_001692"
     CDS             7462..8622
                     /locus_tag="PWT25_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL1021780.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="PWT25_001693"
     CDS             8698..8976
                     /locus_tag="PWT25_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL1021781.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="PWT25_001694"
     CDS             8957..9328
                     /locus_tag="PWT25_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL1021782.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="PWT25_001695"
     CDS             9344..9748
                     /locus_tag="PWT25_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021783.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="PWT25_001696"
     CDS             9745..10155
                     /locus_tag="PWT25_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021784.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="PWT25_001697"
     CDS             10216..10803
                     /locus_tag="PWT25_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1021785.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="PWT25_001698"
     CDS             10876..11343
                     /locus_tag="PWT25_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDL1021786.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="PWT25_001699"
     CDS             11389..11697
                     /locus_tag="PWT25_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021787.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="PWT25_001700"
     CDS             11892..18626
                     /locus_tag="PWT25_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL1021788.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="PWT25_001701"
     CDS             18630..19502
                     /locus_tag="PWT25_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL1021789.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="PWT25_001702"
     CDS             19499..20548
                     /locus_tag="PWT25_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021790.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="PWT25_001703"
     CDS             20552..21376
                     /locus_tag="PWT25_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021791.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="PWT25_001704"
     CDS             21394..>22031
                     /locus_tag="PWT25_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL1021792.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DANJLF010000040        22026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000040 DANJLF010000000
VERSION     DANJLF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(61..1371)
                     /locus_tag="PWT25_001705"
     CDS             complement(61..1371)
                     /locus_tag="PWT25_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL1021793.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="PWT25_001706"
     CDS             complement(1383..2801)
                     /locus_tag="PWT25_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1021794.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="PWT25_001707"
     CDS             complement(3041..4609)
                     /locus_tag="PWT25_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL1021795.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="PWT25_001708"
     CDS             complement(4613..4894)
                     /locus_tag="PWT25_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL1021796.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="PWT25_001709"
     CDS             complement(4891..5814)
                     /locus_tag="PWT25_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL1021797.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="PWT25_001710"
     CDS             complement(5811..6689)
                     /locus_tag="PWT25_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL1021798.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="PWT25_001711"
     CDS             complement(6676..8013)
                     /locus_tag="PWT25_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL1021799.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="PWT25_001712"
     CDS             complement(8016..9119)
                     /locus_tag="PWT25_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL1021800.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="PWT25_001713"
     CDS             complement(9193..10701)
                     /locus_tag="PWT25_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1021801.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="PWT25_001714"
     CDS             complement(10667..11359)
                     /locus_tag="PWT25_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL1021802.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="PWT25_001715"
     CDS             complement(11381..12355)
                     /locus_tag="PWT25_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1021803.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="PWT25_001716"
     CDS             12655..13527
                     /locus_tag="PWT25_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1021804.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="PWT25_001717"
     CDS             complement(14109..16307)
                     /locus_tag="PWT25_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021805.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="PWT25_001718"
     CDS             16539..17531
                     /locus_tag="PWT25_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1021806.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="PWT25_001719"
     CDS             17591..19012
                     /locus_tag="PWT25_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL1021807.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="PWT25_001720"
     CDS             19005..20504
                     /locus_tag="PWT25_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL1021808.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="PWT25_001721"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="PWT25_001721"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL1021809.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DANJLF010000041        21988 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000041 DANJLF010000000
VERSION     DANJLF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..395
                     /locus_tag="PWT25_001722"
     CDS             <1..395
                     /locus_tag="PWT25_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021810.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="PWT25_001723"
     CDS             398..1147
                     /locus_tag="PWT25_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021811.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="PWT25_001724"
     CDS             1159..1428
                     /locus_tag="PWT25_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021812.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="PWT25_001725"
     CDS             1433..1597
                     /locus_tag="PWT25_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021813.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="PWT25_001726"
     CDS             1590..1892
                     /locus_tag="PWT25_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021814.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="PWT25_001727"
     CDS             1889..2050
                     /locus_tag="PWT25_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL1021815.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="PWT25_001728"
     CDS             2047..2352
                     /locus_tag="PWT25_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021816.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="PWT25_001729"
     CDS             2352..2708
                     /locus_tag="PWT25_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL1021817.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="PWT25_001730"
     CDS             2695..2913
                     /locus_tag="PWT25_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021818.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="PWT25_001731"
     CDS             2910..3329
                     /locus_tag="PWT25_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL1021819.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="PWT25_001732"
     CDS             3326..3883
                     /locus_tag="PWT25_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL1021820.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="PWT25_001733"
     CDS             3880..4176
                     /locus_tag="PWT25_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021821.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="PWT25_001734"
     CDS             4253..4666
                     /locus_tag="PWT25_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL1021822.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="PWT25_001735"
     CDS             5012..5386
                     /locus_tag="PWT25_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021823.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="PWT25_001736"
     CDS             5423..5590
                     /locus_tag="PWT25_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021824.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="PWT25_001737"
     CDS             5616..5960
                     /locus_tag="PWT25_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL1021825.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="PWT25_001738"
     CDS             5965..6246
                     /locus_tag="PWT25_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021826.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="PWT25_001739"
     CDS             6349..6663
                     /locus_tag="PWT25_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL1021827.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="PWT25_001740"
     CDS             6641..8335
                     /locus_tag="PWT25_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL1021828.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="PWT25_001741"
     CDS             8355..9533
                     /locus_tag="PWT25_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL1021829.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="PWT25_001742"
     CDS             9496..10182
                     /locus_tag="PWT25_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL1021830.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="PWT25_001743"
     CDS             10182..11342
                     /locus_tag="PWT25_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL1021831.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="PWT25_001744"
     CDS             11352..12227
                     /locus_tag="PWT25_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021832.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="PWT25_001745"
     CDS             12224..12535
                     /locus_tag="PWT25_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021833.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="PWT25_001746"
     CDS             12525..12878
                     /locus_tag="PWT25_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL1021834.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="PWT25_001747"
     CDS             12868..13269
                     /locus_tag="PWT25_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021835.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="PWT25_001748"
     CDS             13262..13666
                     /locus_tag="PWT25_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021836.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="PWT25_001749"
     CDS             13678..14286
                     /locus_tag="PWT25_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1021837.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="PWT25_001750"
     CDS             14306..14668
                     /locus_tag="PWT25_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021838.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="PWT25_001751"
     CDS             14671..14853
                     /locus_tag="PWT25_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021839.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="PWT25_001752"
     CDS             14870..18289
                     /locus_tag="PWT25_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL1021840.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="PWT25_001753"
     CDS             18340..19077
                     /locus_tag="PWT25_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1021841.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="PWT25_001754"
     CDS             19087..21378
                     /locus_tag="PWT25_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1021842.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="PWT25_001755"
     CDS             21402..>21988
                     /locus_tag="PWT25_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021843.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DANJLF010000042        21773 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000042 DANJLF010000000
VERSION     DANJLF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(152..550)
                     /locus_tag="PWT25_001756"
     CDS             complement(152..550)
                     /locus_tag="PWT25_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL1021844.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="PWT25_001757"
     CDS             complement(547..1320)
                     /locus_tag="PWT25_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL1021845.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="PWT25_001758"
     CDS             complement(1317..2000)
                     /locus_tag="PWT25_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL1021846.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="PWT25_001759"
     CDS             complement(2167..3054)
                     /locus_tag="PWT25_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1021847.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="PWT25_001760"
     CDS             complement(3051..3920)
                     /locus_tag="PWT25_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1021848.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="PWT25_001761"
     CDS             complement(4057..5343)
                     /locus_tag="PWT25_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1021849.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="PWT25_001762"
     CDS             5616..6194
                     /locus_tag="PWT25_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1021850.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="PWT25_001763"
     CDS             6194..7123
                     /locus_tag="PWT25_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1021851.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="PWT25_001764"
     CDS             7120..8856
                     /locus_tag="PWT25_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL1021852.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="PWT25_001765"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="PWT25_001765"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL1021853.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="PWT25_001766"
     CDS             complement(9752..10261)
                     /locus_tag="PWT25_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL1021854.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="PWT25_001767"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="PWT25_001767"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL1021855.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="PWT25_001768"
     CDS             complement(11562..12653)
                     /locus_tag="PWT25_001768"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL1021856.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="PWT25_001769"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="PWT25_001769"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL1021857.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="PWT25_001770"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="PWT25_001770"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL1021858.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="PWT25_001771"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="PWT25_001771"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL1021859.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="PWT25_001772"
     CDS             complement(16616..17590)
                     /locus_tag="PWT25_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL1021860.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="PWT25_001773"
     CDS             complement(17571..19454)
                     /locus_tag="PWT25_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL1021861.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="PWT25_001774"
     CDS             complement(19472..21697)
                     /locus_tag="PWT25_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL1021862.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
BASE COUNT         6209 a         4795 c         3862 g         6907 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgt
//
LOCUS       DANJLF010000043        21699 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000043 DANJLF010000000
VERSION     DANJLF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..1055)
                     /locus_tag="PWT25_001775"
     CDS             complement(<1..1055)
                     /locus_tag="PWT25_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HDL1021863.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNL"
     gene            1312..4701
                     /locus_tag="PWT25_001776"
     CDS             1312..4701
                     /locus_tag="PWT25_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL1021864.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            4704..6125
                     /locus_tag="PWT25_001777"
     CDS             4704..6125
                     /locus_tag="PWT25_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL1021865.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6122..7999
                     /locus_tag="PWT25_001778"
     CDS             6122..7999
                     /locus_tag="PWT25_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL1021866.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8091..8933
                     /locus_tag="PWT25_001779"
     CDS             8091..8933
                     /locus_tag="PWT25_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1021867.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9085..9960)
                     /locus_tag="PWT25_001780"
     CDS             complement(9085..9960)
                     /locus_tag="PWT25_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1021868.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10167..11198
                     /locus_tag="PWT25_001781"
     CDS             10167..11198
                     /locus_tag="PWT25_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1021869.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11287..11589
                     /locus_tag="PWT25_001782"
     CDS             11287..11589
                     /locus_tag="PWT25_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL1021870.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            11768..13783
                     /locus_tag="PWT25_001783"
     CDS             11768..13783
                     /locus_tag="PWT25_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1021871.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            13785..14069
                     /locus_tag="PWT25_001784"
     CDS             13785..14069
                     /locus_tag="PWT25_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1021872.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14095..15468
                     /locus_tag="PWT25_001785"
     CDS             14095..15468
                     /locus_tag="PWT25_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL1021873.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15551..17566)
                     /locus_tag="PWT25_001786"
     CDS             complement(15551..17566)
                     /locus_tag="PWT25_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL1021874.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17588..18802)
                     /locus_tag="PWT25_001787"
     CDS             complement(17588..18802)
                     /locus_tag="PWT25_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1021875.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19023..19967
                     /locus_tag="PWT25_001788"
     CDS             19023..19967
                     /locus_tag="PWT25_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1021876.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20263..21207
                     /locus_tag="PWT25_001789"
     CDS             20263..21207
                     /locus_tag="PWT25_001789"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL1021877.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7393 a         3968 c         4079 g         6259 t
ORIGIN      
        1 agattctgat tcagtagagt ggaataaaat tgaagttctg cacgaaatcc agcatataga
       61 cgtatctggc ggtgttgcat ctgctgtgca aagtaagttg cacggatcgt caaatgagtc
      121 gtatcgtatg ggaaaatacg ggagaaaaaa taaatttgcg ttaataattg aacaacatat
      181 tcttcgtttt tttctttgca atcagcggat gaaaacgaac agtaggacat attttttcgt
      241 atctgctcac tgatttcatt tttttctttc tccgaaaatt ctgaaaaaat cgtattgaca
      301 aaatagttca ctacttttat atagctgctg tacttcaatt ttcctaagag atttttcaag
      361 attaagaagt cttttttatc gatatttatc aactgtacat aagagtctcc agctttccat
      421 tcaaatggag ttccttggga ttctctcatg agatgcacaa caaaatacat aataaagaaa
      481 gattgctcat atgggctttc ttcctgaaac aaattcatct gacaattatt cttgaccata
      541 atttctttga tcttcttttt ttcgattgga aatggcaacg tgcgtcttcc gtagtaactt
      601 aaaaagaaaa aggtaacaaa gatacgcaac tggagctcac ttttaaggct agttaactga
      661 aaaccttggt caacgatcac ttttaataaa tagctttcta aaatttgatt gcattgtttc
      721 aacttacgtg taaaagaaga ccggctgatg gaaatttttt cacaataata ctcaatcgtt
      781 ttacccggat tgacaataat ctcatttaat aaacgactgt ttgtagaact ttgcgacatt
      841 tccatcagaa catttaaata agctggtaca ttcaaatgtt caagacgata ccccaatttt
      901 tgtgaagatt gaatcgttac atccgttccc cagcggattt tgatctcttc gatatacctt
      961 acaaatgtag attctgaact accaattcgt ttggcaagtt cttttttagt catccacttg
     1021 tcagaaatgg acagaattcg aatgattttt tccatctttc tatagtttcc ttccaacaac
     1081 ctgatcatat gtttttctcc ctcaaccctt ttctttcaat gaaacgctca ttaatcgaag
     1141 gaatcaactt cataaatgaa accaattggt atgaaaattg gtgtgattcc ctttagcgcc
     1201 cttcgtataa taattatcat attaaaataa ataaaattat gtaacgcctg tttattttaa
     1261 taattttcac tattcacgtc aaaacagaaa aaaagaaaaa ggaggggaaa aatgacgacc
     1321 acagggaaga aactgaaagt tattttcatg ctgataatat tgagtttatc aaactttgtg
     1381 ccattatctg caatagcaga cactacagat gatccaacag ttttagaaac aatttcagct
     1441 gaagtcattt cggatcagtc tggaaaaaaa gcactgaaca tcaagctaaa tgcgaataac
     1501 accagtgctg aaaagataga aaaagaaatt ggtctagtcg aaaattactt aagtgatgtg
     1561 gaaagaaaag aaggagatgg ctatgcttat caggtaaata gcgggaaaat tacgttggaa
     1621 atctcatcaa acactaaaca aactatcgat ctgagttttc caatcgatcc agcactttac
     1681 cacagccagg caaacaagct gatcgtcgat aataaagaat atgacattat tgatgagaca
     1741 gaaaataaga aagatacaga tgtgtcagta ccaaagccag acgaaataga agaagaatca
     1801 tcaaaagaaa acgaaaattc tgtcagccca tttacattgc ctacattatc cttgccagct
     1861 gtgagtgtgc catctaatca aacgattcct acagaatata caacagatga tcagggcact
     1921 tatcctaaag ccagttggca acctacagga aatacaaatg ttcttgatca tcaaggcaat
     1981 aaaaacggaa caaatcaatg ggatggtata aattcttgga atggagatcc taatgatcgg
     2041 acccattcgt atatcgaata tggaggaacc ggtaatcaag cagactatgc gatacgaaag
     2101 tatgcaaagg aaacaagtac acccggattg tttgatgttt atttgaatgc tcgtggaaat
     2161 gtacaaaaag atatcacgcc tcttgatctc gtattggtcg tagactggtc aggaagtatg
     2221 aacgacaata atcggatcgg tgaagtaaag attggtgtcg atcgttttgt cgatacttta
     2281 gcagatagcg gtatcacaga caaaatcaat atgggatatg tcggctactc aagcgaagga
     2341 tatagctaca gtaacggtgc agtacagatg ggttcatttg attcagtgaa aaatcaagta
     2401 aaatccatta caccttcacg gacaaatggt ggtactttta cacaaaaagc actaagagat
     2461 gcaggaagca tgctatccgt tccaaatgga cataaaaaag tgatcgtttt gctgacggat
     2521 ggtgtaccaa cattttccta taaagtacag cgggtacacg cacaatcaag cagcaattat
     2581 tacggaactc agttttctaa tacgcaagat cggccgggaa atacttctct aatctcaaga
     2641 atctatgatg cacctgacca aaacaatcta tccagaagaa tcgacagtac gtttatcgca
     2701 accatcggag aagcgatggc actcaaagaa cgaggaatcg aaatacatgg tcttggcatc
     2761 caacttcaaa gcgatccggc agctggtctc tcaaaagcag aagtagagtc tcgtatgcga
     2821 caaatggttt catcagatga aaaaggcgat ctttactatg aatcagctga tcatgcaaca
     2881 gatatctctg aatacctagc caaaaaagct gtacagatct cagcaactgt aagcaatgga
     2941 caaataaatg atccaatcgc agaaccattc atttatcagc ctggtacact ttcagtcaag
     3001 agtgtgggga caagtcctac aacggtcact ccatctattt ccatagaagg aaataccatc
     3061 aagagcaatc agatctattt aggaaaagac caagaaatcc aaatccatta ccaagtgaga
     3121 atccaaacag aaaatgagga cttccatcca aatttctggt atcaaatgaa cggcaggaca
     3181 actttccagc caaacattga taccaatgaa ttagctgaat tcggtatacc atctgctaaa
     3241 gctcccggag tcagtcttca catcaaaaag ttatgggaag aatttgacaa caatctagct
     3301 gatcgtccag atcaagttac ttttgagatt caacgggaac atacgacaaa tgctgcagct
     3361 tggaaaaacg gatatattcg aatcattaaa ccagctaaag atacaacaaa tacgtgggaa
     3421 cgtgcagaca ttgacaaatt atctgcaaat agcggagaaa gttatcaaga gatattatca
     3481 ctacctcaat acaataatca aggtcaagca ttcagttacc aaacaatcaa agaattacct
     3541 gtaccaggat acgattctca acaaatagat gcaatgacat ggaaaaatac taaacaattc
     3601 acaccgttaa acttgaaaat aacgaaaaat tcctctacag gtgaaaagga tcttattggc
     3661 gctgttttca aattaacagg agattctatt gatactttac taacagatca tggcgacgga
     3721 acctattctc ttccagaaaa tgtcaaattg caaaaagaaa tgacctatac gctgacagaa
     3781 acaaaagctc cagaagggca tggattaagc aaaaagacta cttgggaaat caagatcgct
     3841 tctgatggta cggtaaccat tgatggaaaa acagtcacta cttccgatga tacgatccag
     3901 ttgactattg aaaatccttt tgttgaagtt cctgtagcag tacgtaagta tgcgatgcaa
     3961 gggacggaca aagagataaa tcttaaagga gcagcatttt ccctacagaa aaaagaagca
     4021 aatggtactt atcagccaat tgacagccaa acaacgaatg aaaaaggtct tgccagtttt
     4081 gattcactca cacctggtaa atatcgagtc gttgaaacag ctggtcctgc cggatatgat
     4141 acttcgccgg gaaattatga attccaaatc gataaatatg gaaaaatcat ttacacggga
     4201 aaaaataccg agatgacaaa taatgtatgg acgctcactc atcaaaatcg actaaaagcg
     4261 tttgatctaa cggtacacaa aaaagaagac aacggacaga cattaaaagg agcaaaattc
     4321 agactgcagg gaccagaaat ggacttagaa tcgccaaaag atggacaaga aacagatacc
     4381 tttctattcg aaaatttaaa acctggaact tatacgctga ccgaaacttt tacaccagaa
     4441 ggataccaag gtctaaaaga gccagttact atagttatac acgaagatgg gtcaattcaa
     4501 gtggatggac aagatcatga atctgttctg tcaccaggag ccaaaaacaa ccagatttct
     4561 ttagacatca cgaatcaggc aaaagtacca ttacctgaaa cgggaggaat tggccgttta
     4621 ggaatctatc tagtagggat gattggttgt gcgttttcta tttggtatct ttttttgaaa
     4681 aaagaaagag ggggcagcta aatatgaaaa aacttggttg gcttagtatg tgtctcttct
     4741 tgttactatt taaaccagct tttactcagg tagcaacaga aacagaaaca gaaatggttc
     4801 agattacttt acacaaattg cttttcccaa acgggcaact gccgaaaaat catccaaatg
     4861 acggacaaga aaaagcttta ttacaaacgt atcgaggatt aaatggtgtc acattccaag
     4921 tttatgatgt cacagattct ttttaccatc tacgggaaaa gggcaaaacg gtagaagaag
     4981 cacaagcaga gatcgcaaaa aacggtgcgt cttccggtat gtttaccgca gaagcaacaa
     5041 ctacaactct taacaacgaa gatggtatcg cttctttttc tctggccgct aaagatcaag
     5101 aaaaaagaga taaagcgtat cttttcattg aatccaaagt accagaagtc gtcaaagaaa
     5161 aggcagagaa tatggtagtt gttcttcctg tacatggaca aaacaatcaa aaactttcaa
     5221 ctatccattt gtatcctaaa aatgaagaaa acgactaccc tgatccacct tttgagaagg
     5281 tattagaaga gcctagaaat gattttacga ttggtgaaaa aatcacttat tccttgcata
     5341 cgacaattcc tgtaaatatc cttgactatc aaaagttcga attgtcagat agtgcggatg
     5401 aagcattaac gtttttacct aatagtttaa cgatttcatc gaatggagaa aagctgacag
     5461 aaggctttgt catacacaag aaacctcacg gatttgatgt tttattttcg atcccttcgt
     5521 tggaaaaata tgctggaaaa aaactgacca tttcttatca gatgcagcta agcagtacag
     5581 cacaggcgaa caaggaaatc aacaacaacg gaacactgga ttttggtttt ggtgtcagta
     5641 caaagaaagt ctctgtatat acagggagta agcaatttgt caaaatcgag acaaataaac
     5701 cagataaacg attagctggc gcagtattcc ttattaaaaa caaagcagga aattacctcc
     5761 agcaaacagc caacggatac aagtggacaa agaacgaatc agatgcgctt cacctgattt
     5821 ccgataaaaa tggcgctttt tcaatttccg ggttgaaaac aggaagttat cgattaaaag
     5881 agatcgaagc accttctggt tatattttaa gtgaaacaga aattccgttt accatttcaa
     5941 cttttctttc tgaggataaa gaggcggaca gtatattgaa agtagtcaat aaaaaagaaa
     6001 atagccgtcc atttcttcca aaaacaaacg aaacgaaaaa tacactttta ggcgttgttg
     6061 gtatggtatt cgcaagcttt gcaatctggt tgtttatcaa aaaaagaaca ggagtgaaaa
     6121 aatgaaaaat cataaaaaaa taaacgttat gttaggagtc cttttcctta ttttaccatt
     6181 actcacaaac agcttcggcg caaaaaaagt gtttgcagag gagacagcag ctcaagtcat
     6241 ccttcataaa aagaaaatga ctgatttacc cgatccttta atccaaaaca gcgggaaaga
     6301 aatgagcgaa ttcgatcaat accaaggatt agccgatatt tcattttcag tttataacgt
     6361 cactcaagaa ttttatgcgc aacgagataa aggagcgtcc gtggatgcag caaaacaagc
     6421 agtccagtct ttgactcctg gtacaccagt tgcttcagga acgacagatg ctgatggaaa
     6481 tgtcacttta tctttaccta aaaaacaaaa tgggaaagat gcagtctaca cgatcaaaga
     6541 agaaccaaaa gacggagtgt cagctgccgc aaacatggtt ttagctttcc ctgtatatga
     6601 gatgatcaaa caagcagatg gctcttataa atacgggaca gaagaactag atactatcca
     6661 tctctaccct aaaaatacag tcggtaatga tggaacgttg aaagttacaa aaatcggtac
     6721 tgccgaaaac gaagcactaa atggagcaga atttattatt tctaaagaag aaggaacacc
     6781 aagcgtcaaa aaatacatcc aaagtgtcac agatggattg tacacttgga caactgatca
     6841 aaccaaagcc aaacatttca ttactggtca ttcttatgac atcggcaaca atgactttgc
     6901 cgaggcatct attgaaaaag gccagttgat cgttaatcat ttagaagttg gaaaatataa
     6961 tttagaagaa gtaaaagctc ctgataatgc ggaaatgatt gaaaagcaaa caatcacgcc
     7021 ttttgagatc ctggcaaata gccaaacacc agtagaaaag accatcaaaa atgatacgtc
     7081 taaagttgat aaaacaacac ctcaattgaa tggaaaagat gtcgcaatcg gtgaaaaaat
     7141 tcaatatgag atttctgtca atatcccatt aggtatcgct gataaagaag gaacgcaaaa
     7201 caagtacaca acattcaaac ttatcgatac tcatgacgct gctttaacat ttgataatga
     7261 ttcttcagga acgtatgctt atgccttata tgatggaaat aaagaaatcg acccagtaaa
     7321 ttattctgtc actgagcaaa cagacggatt cacggtttca gttgatccga attatattcc
     7381 ttcattaact cctggcggta cattgaaatt cgtttactat atgcatttga acgaaaaagc
     7441 agatccaacc aaaggatttt ctaaccaagc aaatgtcgat aacgggcata caaatgatca
     7501 aacaccaccg tcagtcgatg tcgttactgg gggcaaacga tttgttaaag tagatggtga
     7561 cgttacatca gaccaaacac ttgctggagc agaattcgtc gttcgtgatc aagatagtga
     7621 cacagcgaaa tatttatcga tcgacccatc cacaaaagcc gtcagctggg tatcggcgaa
     7681 agaatcagca acggttttta caaccacaag taacggttta atcgatgtga caggtctaaa
     7741 atatggcacg tactatctgg aagaaacgaa agcgccagaa aaatatgttc cattaacaaa
     7801 ccgtgtagca tttactatcg atgaacaatc ttatgtaaca gcaggacagt tgatttctcc
     7861 tgaaaaaata ccaaataaac acaaaggtac acttccttca acaggcggta agggaatcta
     7921 tgtgtatatc ggtgcaggag tagtccttct actgattgct ggactgtact ttgctagacg
     7981 caagcacagt cagatttagt ctttttgcaa gaattggaaa aaggagcagc ggattacgca
     8041 aatgctcctt tttcttttat tatcaaacag tcatcacaag gagaacacaa atgaaaccaa
     8101 agaaaaggaa acttctagat ctgctgatgc ttttcttgct tctctcagga atagggatac
     8161 tagcttatcc atttgttagc gacgcgctga ataattatct ggatcagcag atcatctctc
     8221 attatcagca acaagcggtc aaagaaaatg aagaagtcat ggctaaaatc caagaaaaca
     8281 tgacaaagaa aaaccagcaa ttagcaaaag aaggtggaaa tccaggtgca gatcccttta
     8341 ctaagaaaaa agaacctgcg aaaaaggata gaacctattt tgagaaacat acgatcggta
     8401 ttttgacgat ccctgagatc aatgtccatt tgccaatttt tgatgaaacg aatactgtgc
     8461 tgctagaaaa aggtgcctca ttattggaag gaacgtctta tcctacgggg ggagaaaata
     8521 cacatgccgt tatttccagc catcgaggac tgcctcaagc aaagttgttc acagacttac
     8581 cgaaactgac tttaggtgat tctttctata tcgaaatcaa tggacggaca ctcgcttatc
     8641 aagtcgatca gatcaagacg gtagaaccga ctgatacaaa agatctgaga atcgaaaaag
     8701 ggcaggattt cgtgacactc ctaacttgta caccttatat ggtcaacagt catcgcttac
     8761 ttgtcagagg gcaccgggtt ccttatcatg caaaagaagt tcaaaaagaa gtacagaaag
     8821 tatccgagaa caaacgattt ttcctgtatg gattgttagc tgcatgtata ctgattttcc
     8881 tccttcttat atatcttttt cgtcaatcgt tcaaaataag aaacagaagt tagctattcc
     8941 tcatttctat aatatatcta ttcgaatcgt tccgtaaaca aacaggctga ggtttgaaaa
     9001 agaaaacttc agcctgtttt tatgatgata tcaaagagag cctatcaaac actatctttc
     9061 ttattactaa atgaggatct tttcttaagt tttccgatat tctttaagaa ggcctttttg
     9121 catcaattct tgtttgtttt tcaagttgcg gatattctct tcaaaatttt gagaatatat
     9181 cactgagaat aacgtattta atacgtaatc gaaggcaatt tgagaggaga aagtcgctac
     9241 tttagcaaaa tcatattcct cctgcgatat aacaagacac atatcagcct gtttggccat
     9301 ttctgaatgc tgatttccgg taatcagcaa ggtaggggcc tctacatgct tcaaataggt
     9361 cattattcgt tcataatgat ggatgcgacc gctgtagcta ataaaaatgg cgcaatcctc
     9421 gctagtcaaa ttggctgcat tccaagaaga atcactgtat tcttcagcca tgatcaaaaa
     9481 cttattcaac ttgaccattt tattctggaa tttccttgcc gtgatttgag aatcaccttt
     9541 tgcaaaaaga aagattcgct gtgccttgtt caatacttta ccgatttgat ctaaatcttc
     9601 atgttcaatc tgcaattgtg ctttttttat tgactggatc gataaatccg ccattttctt
     9661 tgcaatttgc tgagtgctat cttcacttga aaaaggaaaa ttaggatcaa tcgtttgatt
     9721 gaactgagca atcaattgat gaacttcccg ccccagctcg aaacggaatt ctttaaatcc
     9781 gctgaatccc atttttttgc ataaacgaat gatagctgaa tgggaagtat acgtagcttt
     9841 tgctaattct tcaatcgtca tgtagactgc agcttcgata ttcctacgga tgtaatcgac
     9901 aatccgtttc tctgtcgtgg taaacaactc ttgttttgtt agtttttctt caataagcat
     9961 gatctgacct ccaaattcta tcttactgat aaacacttgg ttccgctact atctttttga
    10021 tttttatcct tactttgaac attaatttca ccaaaccgaa tgataacaag tgtttttctg
    10081 aactttttta ccagaatagc tattataaat actttctgcg cttttctagg atatactgaa
    10141 aacatcaata aaaggaggca ccactcatgc ttacaatcgc ttatcttggc aatggaaaga
    10201 gtacaaatcg ctatcatttg cctttttcaa gaaaacttac gaacaaaatc aatatcaaaa
    10261 aaattttttc cccatctgaa ccagtgtgga aaacattcga agacatcgaa tatactgatc
    10321 agctgtctga tctttgggat gatcctgaga tccaattagt tgtcatcact acacctagtc
    10381 aatttcatta tgaatatgga aaaatggcac tcgaacatgg taagaatgta ctagtagaga
    10441 aaccttttgc agaaacgtca aaggaagcag aagagttatt tgcattagca aaagaaaaag
    10501 gtctatttat ccaatgttac caaaacagac ggttcgattc tgacttcttg acagtccaaa
    10561 aagtaatcga aagcggaaaa ttaggggata ttttagaagt agaaatgcat tatgattatt
    10621 tcagaccgga agtaccagaa aatacgcatg aattttctac tgcgaacagc tatttatatg
    10681 gacatgcttg tcatacagta gatcaagttc tttcttattt cggcgaacca gatgatatca
    10741 gctatgatgt acgtcaactt ttagggaaag gacgaatgaa tgattacttc gatctggatt
    10801 ttttctatgg aaatatgaaa atttcgataa aatccagtta tttccgcatc aaagaacgtc
    10861 caagtttcgt cgtttacgga aaaaaaggga tgttcgtgaa acaaacaaaa gatcgacaag
    10921 aagaacatct gaaaatgttt tacatgcctg atcatgaaga ctttggtatc gatttgcccg
    10981 aacattatgg tgtattgacc tatatagatg aacaagggag ttatcatgaa gaaaaagtaa
    11041 tttccgaggt tggtgattac agccgtgtct acgaagggat ttacgagaca ttagttaatg
    11101 gcgcagaaaa agtcatcaaa gatgaagaaa caatcttaca aatgaaaatt ttggaagaag
    11161 gaatcaaaag cattcaagaa tcagaaggaa aaatatgaca tatttgtaag aatcgggttt
    11221 gttttttgac gcataaactg tcttattgta cgatgaaaaa gtatagaact actaggagga
    11281 accaacatga caaattatga cggtgaaaaa cacaaacgat taagaggttc aatggcccct
    11341 aaaacagaag aagtggcttt agcaggaact aattctggtg aaacagaaga caaagaagaa
    11401 tcattagctg gcacaaactc tggtgaaaca gaaaataaag aaacagcttt atctggttca
    11461 aattctgtag aagaggaaac aaagcctaat gagcccaatc actttgaaaa agatatcgaa
    11521 aaagcggttg aagatgtcaa agaaaaattt gaagaactga cctcaaaaaa taaacatgat
    11581 catcattgat cggacgaaat caattcataa aagcaggaga catttggtca cctgcttttt
    11641 ttctgtatcc attcgataga aatcgttcgg tacagtcgta aaaaaaatcc aactgtaagt
    11701 tgtaccaaac acccctcgat ttaaaagcgc taccatacta gaatataagt aggaggtgaa
    11761 cagcgctgtg aactacaaaa ttagttatct cctgaaaaca ccaagagaaa tcagtctaga
    11821 aaaagcagca gagatcatcg aagtaaatac aagcgaggcg atcaaacaat taactccttt
    11881 tgccgaatat gtggatgata cccgtatccg tataaagaaa ttccgagcaa tcgactgggc
    11941 agaaaaattt ttggatatta ctcgatttga agctgtgtat accgaacaag agcgaagaga
    12001 tcttctttat ttgatggttt attcgactgc aacgtctgtt tctgtttttc attttcaaga
    12061 ttttcttcag gtttctaaag ggacagtcct gtcagatatc aaaaagataa gaaatgaatt
    12121 agaagtggaa gattgtcagc tgtcgtatac taggaaaact ggttttaggc tgctagggtc
    12181 agaagcaata ttaagaagag aagcaaaaaa tcgcactgct cgtttgatgc agtcacttgc
    12241 tggaagattt gggctcacgt atttaaccgc aaccctttcc attgattatt acgccaaagt
    12301 tagagatttg ctagataaaa cgatttcact atcagatctt cgattcgcac caaatcgaat
    12361 ggatgaatta gcttattatt tgagtttgac aaccattcga attggaaaaa catccatgga
    12421 actggtaaaa gatgctgaac tgttggcttc tcttgcagtc actgacatta gtcgacaagt
    12481 attgactggc cttggctatt ccgaacatca aaattcggag attatatttt tctctctttg
    12541 cttagcatcg atcgttgaag gagatgtcca ggagcctgca cttgattttt tgctggaatg
    12601 ttcttcagaa gtcatccatc gcatggaatt cctgatggcc gtagaatttg cttcgtttcg
    12661 tgaattattg atgaatgtct tttatcactt ggtccctgcc tattttcgta tttcctattc
    12721 tttttattta cctaatgtga tgatcgatca aattaagcat caatatgctt ccatctacga
    12781 aatgacaaga aaagcattac ttccgttaga aagacgaata gggaagagca tccctgaaga
    12841 agagattggt tttttcacta tactatttgg cggagagatc agaaaagcgg acgcagaaga
    12901 aagaaataga aaaataagag ccgtgatcgt ctgtccaagt ggcattagct cttcattgat
    12961 cttaaaaagt gaacttcagc aattattccc gatgatcttt tttacagaga ctaattcagc
    13021 tgaccgctta cacgaggttg acgaaacttc ttacgatatt attttttcta ctgtacctct
    13081 cacacttact gataagaaaa agtctctcta tgttctgcat ccaatcatga ctgacttgga
    13141 aaaaaatcag ctgatcaatc aagtacaaag agattggctg ataccaggat tttcaatgcc
    13201 ttctgcccaa gaattgttgt cggcgctact gccttatatc gaattaaaag aaggcgtaga
    13261 cgaagaaaag ctctatcaag tattaaatcg taaaatgaac aaacttttag aaaagaagga
    13321 ggatcaacgt cctatgctat ctgaattatt aacagaagaa atgatccaat tatcggatca
    13381 accaaaaaac tggcaagaag caatcacatt tgccgcacag ccgctattat cagaaaataa
    13441 aatagaagaa agttatatca aagctatgat cgagcgcgtg gagcagtatg gtgcgtttat
    13501 ccatattggc gatcacatcg cattacctca tgctcgtcca gaagatggcg taaacgaagt
    13561 agggatgtct ttgctgaaac ttcaacaacc tgtggatctg cttgataatc cagcccatcc
    13621 cattagtttg ttcatctgtt tagctgctat cgataatgag gcacatcttc gggctttggc
    13681 aaatttaaca aagctacttt ctaataaaca aaatcttcaa gatttattac acgcatcaac
    13741 aaaagaagaa atattagcga tcataagaaa aggagacgaa tgatatgaaa atttttgcag
    13801 tatgtcaaag tggattagga acaagtttta tggtagagat gaacatcaaa caagcacttg
    13861 cagatcttgg agcagatgtg gacgagtttg aagtaagtca tacggatgtt ggaagtgtat
    13921 ctagcgatat ggctgattat ttcttcattg agcattcttt gaaagatacg ctaacttcta
    13981 tacctgatga aaagctcgta cctttgcaat cgatcattga cagcgaagaa gtaaaagaaa
    14041 aagtaacgaa gatcttgtca aaagaataaa aatgattcaa gggaggaact atcaatggat
    14101 aaattgttga atttgtttgt gtcaatcgtc agtcagccct ccatcctcgt ttctttgatt
    14161 gcactgcttg gtctcgttct tcagaagaag aaattttcag aagtgatcca aggaactatc
    14221 aaaacatttg tcggattcct agtactgatc ggcggtgctg gtttaatcag taattcacta
    14281 accccttttg cttcgatgtt ccaaacagct ctgcatactc aaggagtagt tcctagcaat
    14341 gaagcagtag tggcgattgc tttgcaacaa tacggtacac ctactgcttt gatcatgcta
    14401 gtagggatga tcgtgaatat tttacttgct agattcacaa gatataaatt catttttctt
    14461 acaggtcaag caatgcttta tgtttcttgt ctaacagcag tcattttagt aagcgcgggg
    14521 tttactgcaa atttgtggat gatcttatta ggcggggtct tcgaaggaac actattaact
    14581 attacgcctg ctctttgtca accgtttatg aagaacatca ctggaaacaa tcaagttgcg
    14641 ttagggcata ccggaaatat cggttatgct tctagtggat ggatcggaaa gttttttgga
    14701 aacaaagacc gttctacaga ggacataaag attcctaaaa gtttcagctt tttgcgagat
    14761 tctactgtat cgatcatgct cttgatgtcg atcgtatatg tcgcacttgc tttgttagct
    14821 ggtcctggat ttgttgaaag agaactaagc aatggagaca acgctgtaat ctttgcactg
    14881 atccaagctg gaacattcac agctgggttt gttatcgtcc ttcaaggggt tcgaatggtg
    14941 ttggcagaaa tcgttccagc attccaaggg attgctaaaa aactcgtacc cgattctaaa
    15001 ccagcattag atgttcctat cgtttttcca tatgcaccta atgctgtatt aattggcttt
    15061 tttgttagct tctttgtagg aacactatcc atgtttgcta tgataggctt gcaaactaca
    15121 gttatcattc ctggggtagt aggacatttc ttctgcggag ctgcttctgg tgtcttcggc
    15181 aactcgactg gcggtagaag aggggcggta ttaggttctg ctttcaatag cttattgatt
    15241 agttggctgc cattatttat ccttccagta ttaggagatc ttaaaatcgc ttcttctaca
    15301 tttgccgata ctgattatct agtaccagga cttgtacttg ggaaactagg agaatacggt
    15361 caaatagcct tgacagttgg aatcatcgct ttctttttac ttgtcgtatt atctagcttt
    15421 ctttttgccg gaaaaagaac aaacattgtt tctaaagaac agaattaagc aaacttcaaa
    15481 ataaaaattg cttggacaat atcagaagaa gatttaaagt cttctgatat gtcgcaagca
    15541 gttttttgtt ttatgcattt atttttgaat agcttgttaa gacaagtggc tgttttgcta
    15601 gaagaggata acttttacca ttcattttca ctgttccgcc tgttctatca gaagtaatca
    15661 cgatcttatc ttttgttttt tcaagcgaaa tctttccatc tttgataggt agcgtacaat
    15721 tcagtgtttc aaatgaatct agattaggtt gtatcataaa ttcttcataa cctggctttg
    15781 ttggatacaa accgataaaa tagcgtccta aaagatagat tgggcttgct ccccatgcat
    15841 ggcataagct ttttccataa ggatctccat acatactgta cttttcattt cctgtttgag
    15901 tagggtcgta ttcttcccag aaagttaccg catcatgaga taacatccct ccccaataat
    15961 caagaatcgt ttggtagacg atattcgtct gtccgagttt gcataaagca tcttgttcat
    16021 agaatttgaa atatggagta gtaatctgtg tgatagtgtc attcaataag acattcttta
    16081 agatcgattg ctgttggtct ttgttagcaa aatcgaataa aacagcgaaa atattcgcat
    16141 gacgcgtcac atttcttctc ccagattcat agcaatcgat aaaagcgcca agttcttcat
    16201 cccaaaagta gtcaaatagg ttttgtttca atagttgata tttttcttga tagagatgag
    16261 tatgataatt ttcgttctct ccatatgttt cagctaagat atctccgcaa gtgatcatgg
    16321 aatgataggc tttcagtaat aagacttgtt ctgcagcaag cgttccttct ttatccattt
    16381 cagaccaatc aataaatatc caatcttttg ctctcccata aataaaccct aattcattcg
    16441 tttgctccat caagtatctc attaatgatt gcatcttagg gtagacgatg cgtaaaaatt
    16501 catagtcttt cgtcatcata tagtggtttt cgataccaat caaccacaag atcgaatagt
    16561 caacgatagt attcatatgc tgcttgattt cattttgtcc tcttaatgct aatatcgttc
    16621 gtttattgat ttcctcgtca aaaaaaagat attgattgat catgaatgat tgatacgcat
    16681 ctccagacca aatccagcga tctcgtttga taccgtcgat aaaaaacaaa tcactacata
    16741 acttgaaggc ttcttctgat actgaccaga tctgattcag taaagaagaa tcgcttgaaa
    16801 aagcagcttg tttcgaaaga gataaaaatt catgaaaaac ttgaagttga accattccag
    16861 tagaaacctc tggtataaac aaatagcgaa aagcgcgttt acgagctttc atttttcttg
    16921 tcattcctat ttgcttatag tagcaataat cgatatccat agcttctaca tcggactctc
    16981 cataacatag agtgatagat tcgatatggt catccagaga tattagttct acacagccat
    17041 tcactgctcg cccaaaatcg aataagattc ctccgccaat ctccgtttca gtgactggat
    17101 ggacgcattc tttttgataa tagatttgat ttggatcttt tgcgatatcg acataaagcg
    17161 gactccagcc agctggatat tcttcaataa aattacttgc tgtccagcca atatctgatt
    17221 ttacttcgtc tccctcgata aacatcgctg gtagccctga tgtatgtcct acaaagatac
    17281 gaatttttgc tttaccagct ggacaagtca gccattttcc acatggatgt ttttgtccat
    17341 taatttctac atacccaact ccttggatcg tcactttaaa catggaaggt tgatcaagaa
    17401 agtaatatct cttaaacttg acgttccgat ggcaatcatc catgtaccag tacgctggcc
    17461 agtcaaaacc tcgttcttcc cgttggaaat tttgtagcag tccatggcgg atctcaaagt
    17521 ctccaggata ccaaagccat ctatctttat gtttttcatt tgtcatttta cacgacctcc
    17581 atcaaattta ttttgttgtc aattgttctt ttatttcttc atctactctt atcactgtct
    17641 ttctattcgt cctgtgtact actagaaggc taataagcgc gctaatcaac agcaagatac
    17701 tgccattgac gaaaacagca agtgttttac tcccaagaga agcaaaagag agaaagagca
    17761 aaccatatgg tgcaatgaaa gtacctaaat tagcgccaac caataagatt gaagtattga
    17821 aactctccgc gtacttgggc gcttggatac ttacttggtt gaataagtaa ggaatgaata
    17881 acgaaaaaga aattcctgtt aggatcgcac caaacccaat cacatacagg tttatcacag
    17941 tggcaatcaa tacaaagccg attcccatca gtagtaaaga aagtgtaagc atatagcgat
    18001 gtgcttttgt ctgaagccag ccaaaacaaa ctccggcagc catacttgcc aattgcatga
    18061 ttgttaagat attcgctgca ttagtttcgt ttcctagaga ataagtcgtc aggactgtgg
    18121 ctaatttaat cgatacagca ttatatacca cgatgaccag caagagaaaa ataccataac
    18181 taatcatttt aggattcgtt ttgattactt gtcttgttgg ggctgtgcca tgcgattgaa
    18241 gttcagttgt ttcaggtgta ggaacaaaca aaacaaacaa gacaagaata ggtaagctga
    18301 tggcgtagac taaaaaggta tattgccaac cgaactgtac tagctgtcct gcaacaaatg
    18361 tcattaaagc tgcaccaaat ccttgaaatg cactttgaaa accaatcata gaggctcgtt
    18421 catctccgtg ataaaaggca gcaatcatgc tgactgccaa agaattgatc aatccaaagc
    18481 caatacctag tccagctctt gaagctaaga caatactgta actttgtgag aagaggggga
    18541 ggataccaca aatcgatgca atcactaatc cgatgatcac agtttgtttt tgtccgattt
    18601 tcctagcaat aaaactacta atcaaaacaa aaatagtgac cataaaagag ggagcagtcg
    18661 ttaataattc aacagcagat aaagaacgcg tggcataagt agctgacata ctgggaattg
    18721 cagacgtgac tgcaccagca gaagtcaaaa taagagagac tgacagcaga gaaaatttca
    18781 tcatcttttt attcataagc atgttccttt catttttgta agcgcttatc aaaaatatag
    18841 tatgaagtgg ttattctctc aatagcggaa tcacaggaag ttcatgtccc aaccatagaa
    18901 agtctttatg aaaccaaaaa tagctttgca tataatagca gtacagcgtg aactaataag
    18961 cgaaagaaac cgcttacaaa aaggagggag tcaattggaa ctagctgaat tagacatttt
    19021 ttttgcgtaa cgaagaatca atcgaaactc tgcagcgtga acaaggaatg aatatcaatg
    19081 atttaaactt agcttaccat cttcagacac cgccaaaaat ccaccgaggg gatctatttc
    19141 aacagggaaa tattcatatc agtaaacacc gtcgatatgc ggaagttccc gagcatacac
    19201 atgattttgt tgaaatcaat tatatgtata gcggtacatg tttacaaatt attaatggag
    19261 aaaggataga gatggaagaa agtgacgtgg tgatcattga taaagaagct actcaaagaa
    19321 tcgaatacac agggcaagag gatattttaa tcaacatctt attaaaagaa gaaaccttat
    19381 ctacagagat attgaatcat ttggcaagca caaataatct actttcttct tttttgataa
    19441 atgcatcaaa agaaaaaggg gaacatacac aattcattca ttttcgcggc ggaaaaacaa
    19501 gtcgtttgac gcatttgatt gaaaatatgc tgttgattta ttttgataca tacagtcata
    19561 agatccgttc gttaaatctc tatacatcac tattactgat cgaattgact catttattag
    19621 aacaacaaag cttttcatca gcagatggat gcgcagaaaa tgagatgctt agagtattag
    19681 attatatcga taagcatttt acttcttgta cgttaaaaga aacagcagct cattttggct
    19741 acaatccggt ctatcttagt aataaattga aaatgtattt tgatgcttcc tttcaagaac
    19801 tggttctaaa gaaaaaattc gactttgcgt tggaactgat tcaagaaacg agtttttcgt
    19861 tggaagaaat cgctaatcgt atcggatata aacaaacgac ttctttattc aaattattca
    19921 agaagtattc aacatgtact cccaatgaat ggagaaataa acattaagaa gaaaatcttt
    19981 cactaaagca tatgaaggaa aattgtttta ggtgcgattg ctgtcttatt atatcgttgc
    20041 agcgaatttt tttggattta ttttcagttc taaacttttg gttgtattat caggggatat
    20101 aggtgtaaac atttgtcatt tatctgttat gcgtattcag gaattcgttt ccatcaaaaa
    20161 ttataataac aactattata attaacatgt tgataaaatg aataaacaag actatacttt
    20221 tcatgaaagt aacaaatagt tatgaaaagg agctgtttat tcatgaaaaa aacaagtcgt
    20281 aaagtagtaa tcgtcggtac aggttttgtt ggaacaagta tcgcttatgc aatgatcaac
    20341 caaggaatct ctaatgaatt agtattgatc gatgtgaatc aagaaaaagc agaaggggaa
    20401 gcattggacc ttttagatgg tatggcttgg ggcgatgaaa atgtggctgt atggtcaggt
    20461 ggctatgaag aatgtaaaga tgctgatatc gttgttatca cagctggtat caaccaaaaa
    20521 cctggtcaat ctcgtttaga tttagttaaa acaaatgcgt caatcatgcg ccaaatcgta
    20581 aaagaaatca tgggatcagg attcgacggt attatcgttg ttgcttcaaa cccagtagat
    20641 attttgacat atatcgcttg gaacgaatct ggtctgccaa catcacgtgt tatcggtact
    20701 ggaacaacat tggatacaac tcgtttccgt aaagaaatcg cattgaaatt aaaagttgac
    20761 ccacgcagcg tccacggcta tatcttaggt gaacatggtg attcagaagt tgctgcatgg
    20821 tcacatacaa ctgtcggtgg taaaccagtg tttgaaattg tagaaaaaga ccatcgtatt
    20881 gcccaagatg aattagatgt gattgctgat aaagtccgta atgctgctta tgagatcatt
    20941 gatcgtaaaa aagcaactta ctatggtatt ggtatgagta ctgcacgtat cgtaaaagct
    21001 atcttgaata acgaacaagc tgtattgcct gtatctgctt atttaactgg tgaatacgac
    21061 gaaaaagata tctttacagg tgtgccatca atcgttgacg aaaatggtgt tcgtgaagtt
    21121 gttgaattat caatcaacga agaagaaaaa gcaatgttca gcaaatcaac aagtgcttta
    21181 agagaagtat tgaacactgt attataagaa acaatgaata tgaactaaaa atcctaaaac
    21241 tgcttaacag ctgaaatagc tgttaagcag ttttttattg atatctaata ggtaagcaaa
    21301 taaacagaat ggttaaaatt aatcggtccg aaaatgtatc cgtacatatg aacatatgaa
    21361 atttaatctg ttgattacaa taagcgaatc gttatcaatg tttatctgtg gttaattaaa
    21421 aaatccttcc gcaacggcaa atacaatgat tcatatctaa ttcgttcata tttgattaac
    21481 tgaataaaga aactggtttt tatcattcaa ttaaacgaca aaaaaagtaa gaaaaactgc
    21541 aaaatttttt tataattttg cataagcaga ttttgatgga aaaatgacga tttttgataa
    21601 aaaaccgttc gagagaggtt tatcattttt taaatctact ttgtataata tacattatgt
    21661 aaactaaaat atataaaaaa tcagcccact agttctaaa
//
LOCUS       DANJLF010000044        20095 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000044 DANJLF010000000
VERSION     DANJLF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(86..1102)
                     /gene="tsaD"
                     /locus_tag="PWT25_001790"
     CDS             complement(86..1102)
                     /gene="tsaD"
                     /locus_tag="PWT25_001790"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL1021878.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1099..1563)
                     /gene="rimI"
                     /locus_tag="PWT25_001791"
     CDS             complement(1099..1563)
                     /gene="rimI"
                     /locus_tag="PWT25_001791"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL1021879.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(1570..2112)
                     /gene="rimI"
                     /locus_tag="PWT25_001792"
     CDS             complement(1570..2112)
                     /gene="rimI"
                     /locus_tag="PWT25_001792"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL1021880.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2096..2818)
                     /gene="tsaB"
                     /locus_tag="PWT25_001793"
     CDS             complement(2096..2818)
                     /gene="tsaB"
                     /locus_tag="PWT25_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL1021881.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3009..3989)
                     /locus_tag="PWT25_001794"
     CDS             complement(3009..3989)
                     /locus_tag="PWT25_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1021882.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4013..5497)
                     /gene="galT"
                     /locus_tag="PWT25_001795"
     CDS             complement(4013..5497)
                     /gene="galT"
                     /locus_tag="PWT25_001795"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL1021883.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5509..6498)
                     /gene="galE"
                     /locus_tag="PWT25_001796"
     CDS             complement(5509..6498)
                     /gene="galE"
                     /locus_tag="PWT25_001796"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL1021884.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            6746..6913
                     /locus_tag="PWT25_001797"
     CDS             6746..6913
                     /locus_tag="PWT25_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL1021885.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(6975..8786)
                     /locus_tag="PWT25_001798"
     CDS             complement(6975..8786)
                     /locus_tag="PWT25_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL1021886.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(8783..9148)
                     /locus_tag="PWT25_001799"
     CDS             complement(8783..9148)
                     /locus_tag="PWT25_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL1021887.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9311..9706)
                     /locus_tag="PWT25_001800"
     CDS             complement(9311..9706)
                     /locus_tag="PWT25_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL1021888.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(9724..10686)
                     /locus_tag="PWT25_001801"
     CDS             complement(9724..10686)
                     /locus_tag="PWT25_001801"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL1021889.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(10686..10898)
                     /locus_tag="PWT25_001802"
     CDS             complement(10686..10898)
                     /locus_tag="PWT25_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL1021890.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(10919..11617)
                     /locus_tag="PWT25_001803"
     CDS             complement(10919..11617)
                     /locus_tag="PWT25_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL1021891.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(11637..12179)
                     /locus_tag="PWT25_001804"
     CDS             complement(11637..12179)
                     /locus_tag="PWT25_001804"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL1021892.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(12311..13315)
                     /locus_tag="PWT25_001805"
     CDS             complement(12311..13315)
                     /locus_tag="PWT25_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1021893.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(13312..14301)
                     /locus_tag="PWT25_001806"
     CDS             complement(13312..14301)
                     /locus_tag="PWT25_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1021894.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(14298..15104)
                     /locus_tag="PWT25_001807"
     CDS             complement(14298..15104)
                     /locus_tag="PWT25_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1021895.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            15270..16226
                     /locus_tag="PWT25_001808"
     CDS             15270..16226
                     /locus_tag="PWT25_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1021896.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(16303..16821)
                     /locus_tag="PWT25_001809"
     CDS             complement(16303..16821)
                     /locus_tag="PWT25_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL1021897.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(16909..17058)
                     /gene="rpmG"
                     /locus_tag="PWT25_001810"
     CDS             complement(16909..17058)
                     /gene="rpmG"
                     /locus_tag="PWT25_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL1021898.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            17286..17732
                     /locus_tag="PWT25_001811"
     CDS             17286..17732
                     /locus_tag="PWT25_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL1021899.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(17927..19822)
                     /gene="asnB"
                     /locus_tag="PWT25_001812"
     CDS             complement(17927..19822)
                     /gene="asnB"
                     /locus_tag="PWT25_001812"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL1021900.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         5977 a         4314 c         3463 g         6341 t
ORIGIN      
        1 agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt attacttgat
       61 gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga tcagcattca
      121 actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct ccaatcattg
      181 cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca gggagtttag
      241 ctgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca cctccagcta
      301 taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta atcaatacat
      361 cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct tctccacgct
      421 gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa ctgaaatcat
      481 aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc tgatgagcta
      541 gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca actttgtcat
      601 aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat ccatcttctt
      661 gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat ggaaactgga
      721 atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca ggaataagcg
      781 gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc aacgagccga
      841 caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac acaccagctt
      901 ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg ctagctactt
      961 caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg atgttagata
     1021 agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt gattcaatcg
     1081 ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc tcataatgat
     1141 cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac gaaacttttc
     1201 tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa aaacatgcag
     1261 cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc
     1321 ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta ctttcgagta
     1381 tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag agtgaggctg
     1441 ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat aagcattttc
     1501 gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt ttctgcaaat
     1561 caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt cttttgtttc
     1621 agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat aaagtctttg
     1681 tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat tcattatagc
     1741 aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat atgccggtcg
     1801 aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga taaaaccaat
     1861 cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact tcaattctga
     1921 tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt cgacatccag
     1981 cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat cgatttgttc
     2041 gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat acgctctaaa
     2101 ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt tcttctgctt
     2161 ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt tcaactaaag
     2221 cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact gtattaatca
     2281 tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca ctcccgacga
     2341 aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta
     2401 catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca gcgtatacat
     2461 tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg caattagccg
     2521 cgatggtttt cagactagat actcctacta gctctttatc taatgtataa gcaatcgttt
     2581 ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca gaaaccgcga
     2641 ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg attgcaggca
     2701 tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt
     2761 cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat gtaatcatat
     2821 atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt tttattctga
     2881 ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc tggcgggcaa
     2941 attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc ccacagcttt
     3001 tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat aatctttctt
     3061 gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag ctctcccatc
     3121 cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc tacactgaca
     3181 tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc ttgaatcgct
     3241 ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga
     3301 ttcgttttta aatattgctt cactgcttga tatcctgact cgacagaaaa atctgcttcg
     3361 ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc cataattctg
     3421 ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag catagcgata
     3481 tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga cttaaagtcg
     3541 acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt
     3601 tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc gatcgttcca
     3661 tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac gtttgcttct
     3721 tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc aagctcttcc
     3781 gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc atttttacga
     3841 tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt ggctaagcca
     3901 acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga aactcctgcc
     3961 aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac gtttataaaa
     4021 tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat actccagcgt
     4081 cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg gtgaccgtat
     4141 ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga tgatattcag
     4201 ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct ggtttcaaac
     4261 gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc tttttgatat
     4321 gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa acattgttgt
     4381 ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg
     4441 tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca tccgaatatg
     4501 ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc tctgttgatt
     4561 gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc ggataatctt
     4621 ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga cgtcctgcct
     4681 gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca ttcgaaccaa
     4741 caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga
     4801 ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa taagcataag
     4861 gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc cggtgattgg
     4921 tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa agcatgcagg
     4981 taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg
     5041 gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc ggaaatttga
     5101 tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa ttaaagaaat
     5161 actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca ttgacgactg
     5221 aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt tctgctggca
     5281 attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat aagtctacag
     5341 attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct accatcccta
     5401 aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct tcgatcgcaa
     5461 gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct attcttcata
     5521 accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt tgatatctgt
     5581 gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga ctaatcggct
     5641 aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg tcacttctct
     5701 agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat tgaagaagtt
     5761 gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa tcaaatcttc
     5821 tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt ctccataaac
     5881 agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa gatgcgtttc
     5941 tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat tgaagtagcg
     6001 caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt tttccatcat
     6061 caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg tgattggaga
     6121 ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat tcacgttgaa
     6181 ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga aatacatcaa
     6241 tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta ctccttcaat
     6301 cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc cttcataaaa
     6361 atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta ccacagctac
     6421 ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac cggctccgcc
     6481 tagtactaaa attgtcataa aaaacactcc tttgattagt aaaattgtgt ttgttgattc
     6541 tatataattt aaaaaacgaa gtttactttt atggtgaaaa caaacaaagt aaaatttcgt
     6601 aaattcatat ggtaaattta taataaaaca tttagtaaac attttcaagg gttttttgca
     6661 aaggtttaca aacaaacgta aaactagcct ttttttctct tttgttccat aatagatata
     6721 acaaataata aatgaggtgg attcaatgaa aaaatttgta tctggaatat tagtaggaag
     6781 tctggcaaca gccgctgccg ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga
     6841 tccaattgac gaaaaagaag caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg
     6901 cgtttctcgt taataattat ttaaaaatac gcatgaagac tttctaaaaa acagataagt
     6961 catcatgcgt atttttattt gtcagtcaat ataagttgtg ctattttatc tgcctgtctc
     7021 agattccatg gatctgtata aggtctagct actgctgcag ttagccaatc cacaccttca
     7081 acaggtattc catagcaata aaagtcagac tggatttccc ctgaagcatc aatcacgcga
     7141 ttcgtacctc gctcaacatc tatagcttct gaatgataac ttcctgtatt atctgaaaat
     7201 gaaaaagggc gaacaagttt gttttccttc aaatttttta tgacaggatt acttgaacga
     7261 gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc gactgctgat ttctttatct
     7321 tgtagagaaa taacatagcg gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa
     7381 tgaaaaatac ccgcatctat cagtgctgct aattctttcg tccgaatgat tggcggaccg
     7441 atcgttaaaa agctatttag atgagtaaac catccccata gtatttcttt gtattcctta
     7501 gcagaaaaaa cgtgccattc aatagcctgc cggattggat cacgccagtc ttttaatgca
     7561 tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct
     7621 tcaataaata ttcggctagc ttctgaaaag ggctcattct gataggcaag aggagtttct
     7681 aaatacgacc aatcccatct ataagggatt agtgctggat gctttccgag acactcctct
     7741 gaagagaggg agagaaaatc atgctcaaaa tttttctttg gaatcggcaa gtgatattct
     7801 tcgatgattt tcttgtaata aaacaactca acgtcttttt tcagatagtc aaaaaatgtt
     7861 tcgccagtca aaaatcccga ctgatgccat tgctccaggt tttcttttgt tagtaaccgt
     7921 ggccatgcca ttgctccgcc ttgcttttga tttcttcctc gagggtaata agggagcccc
     7981 cttcttgatc cactgtatac aatcggttcc ttgcctgatg gatgatagac cagttttccc
     8041 ttttcgttct cgaagcgccc tcctcttgat actgtaaaca atccgacata atcaaaaaaa
     8101 cttaagccta gacctcttaa aaagacattt tctttaggcg gtatcttcga aagatctaca
     8161 tctgcaggat tagaaggaag ctgatacagt aaaacatttt tctttgcgta gtctacccac
     8221 actctttctt cttctgtctc atcattttcc cagtgaccgc ttgctaaaac aagctgatcg
     8281 atagaatagc tttgctgatc tgtctgtaaa agataaccat cttttctttt gcttactttt
     8341 tttactaaag aataaacaaa ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa
     8401 aaccagcgtt gatagagtcc gtataaacat cgagtagatt gctgatcttt ttccagacga
     8461 ttgatttctt ctataattag ttgcttattt ttggttaccg tctgttcgat ataatctgta
     8521 gcaggtcctt tactccattg aaacaagttg ggtccctgtg ttaccaatcc tccactggac
     8581 aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag aattcattaa taactccggt
     8641 ggctgattca accgccaaac tcgacctcca ggaccattgg gatcgaaaag aaaaagttca
     8701 agagaatgtc tgtcctgatt attatggaga atcctttctg ctacacttaa tcctctaggt
     8761 ccggcaccaa tgatccctat tttcatttgc atgcctccat ttcgataaat tttttcaata
     8821 aaacagcatg gttctgatgt tcattttttg caccataaac aaaagtaatg actggatgtt
     8881 ctttcataag ttgactgagt ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt
     8941 cttgctggta tttctttgca aatgaagcaa atcgttctgg tacatggtca aaagttttgc
     9001 gtgcttcatt actaggtgca atttctttca accacagatc taactgcgct ttttcttttg
     9061 aaattcctcg tggccagaat ctatccacga gtattctata gccatcctct ttttcagcat
     9121 caaggtaaac acgttttaat ttgatcatag ttgtttcctt ctttctacct tttcgcttag
     9181 cttatatatg tagataatag cacaaaataa aaaaaatgaa cggcacaact atccattgcg
     9241 ccgttcatgt gatcttggtc aaaatctaat gattgattcg gtttttaaag ctgtcataca
     9301 atcatctgtt ttatactttt ttacttttga tttttccaga ccatccgtca tatccaccct
     9361 taagtaaata aatctcttga taacctgctt tacgtaattt atttgctgta cggatgctca
     9421 aacttttctt ttgatcgtaa atatagacag gctggtcttt tcgaatagat cctaatgaat
     9481 ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat atgcccagca tcaaacacat
     9541 ctttttcgcg aacatcgatt acttgtgctt ttctcattcc ttctcgaaat tcttcttctg
     9601 tcaaagtcgt tgctgaacgt ttggccatta ctcgtagata gacttcccaa aggatgattg
     9661 caagcacaat caatagcaaa atgatattaa ttacccataa aaccatgtaa tgcctccgtt
     9721 tttttaagca aatttcaagg ctaatgaggc agcaccaatg acaccagctt cattaccaag
     9781 ctctgctaat ttgatttttg ttgattctct tacttgcggg aaagtaaatt ctttaaaata
     9841 tttctctaca cggctacgta aaaattctcc tgctgccgat actcctccac ctaacacgat
     9901 acttgacgga tttaatgtat tccccaagtt tccgcaagct aaacctaagt agaagcatac
     9961 gcgatcaaca accatcaatg caaacggatc gtcttcttga gccatttcaa agatatcttt
    10021 acttgttact tcttctccgt tgtctagcat tgctttcagt tttgaatccc ctgcgtattc
    10081 ttctgccaag aaacgaccta ggcgtaccac acctgtagcg ctagatacag tttctaaaca
    10141 tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc acagtgatat gtccgatttc
    10201 tcctccagct ccgctcacgc cgtgaatcaa atttccgcca gcaacgatac ctccgccaac
    10261 acctgttcct agagtaacga atacaacgtc tggatcgttt tctccagcac ctttccatcg
    10321 ttcacctaaa gcagcaacat tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc
    10381 ctcgatcagt tttttcacaa attgaacttc tgtccagttt aagttatacg caccaataac
    10441 cgttccagct tcgctgtcca cgcttcctgg ggtacccatg ccgattccga taaagtcttc
    10501 ggctttcata ttatataaat tcaaacgatg attgattgat tcaatgattt caggaacgat
    10561 atgcttacca tcatccaaaa tattggtatc gatgctccat ttttgctgaa tctctccttc
    10621 tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa tcaatcccaa taatcttctt
    10681 gtccattaca aatttccttt ctttcggctt tcttcaattc ggtgctctct ttttaatatg
    10741 cgccacgctg tcatgtatgt atcatgatca atcaaacggc tatcatacag attacgcaat
    10801 tcgatagtca ttaattcaat gtcatagatg cgtgcaccca catagataaa gatgccaaat
    10861 tttttgaaca gctgctgtac atcatataag ctctccatct ttaagcactt ccttcatatt
    10921 atacaagtaa accatatttt tttaaaccca gtaaaataca tattacaaaa agaaatacaa
    10981 aaactagtgt agaaagaatc ctttggtgta tcgaatagct tgtctgttgt ttcggtagac
    11041 ccaaaacatt ccctaacact aatccgccaa ttagcccacc gacatgtccc caaacatcta
    11101 cagaggtatc cagaagacca aaaacaaatg tcaacacaat aaataaaaga aaatgtctga
    11161 ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc taagataaac aacgcaccga
    11221 acaaaccgaa tatcgaagta cttgcaccac cagacagaac attcgcttcg ttaaaagcaa
    11281 agcttgctgt atttcccatt atcccgctga acatataaat caagaaaaaa cgccaatggc
    11341 catagattgc ctcgatttgc tgccccataa aatacagtat cagtgagttc attacgaaat
    11401 gcatcagtcc aaaatggatg aagattggtg tcactaaacg ccaccattca ttgaagtgaa
    11461 caagataagg accatacatt ccggcataat aagggatctc aagtcgcgga aataattcca
    11521 tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata ggtccataac ggtcgtctta
    11581 accatgcacg caattttatt tgctgttgat aattcatgaa aacctcccat ttcattttag
    11641 tcaataaaca attgttggat cttctgatca aagctctccg gttgccattg ttcattcaat
    11701 tgttcacgaa aaacaaggct acatgttttt ttatgaaaat tatttaaata ccggtcgtaa
    11761 taaccaccgc caaaaccgat tcgataaccg gctctattgt agataatacc tggtacaatg
    11821 aggagatcga tcatctccgg caaatagagc ttctctgatt gtggctcaag aacaccaaaa
    11881 ctcgaacgta caagaacgga ctgttcatcc atttcataaa acgccatctg acgtttcggc
    11941 aaagttttag gtacaactat tttcttttgt tctttttctg ctcgttggat aaccggctgt
    12001 gtatccagtt caaattcatt cgataaagtc gtccctatca cttttgctga cttccagtac
    12061 tcgctggcga atagctgttt atagattcgc tgttcttttt cctttttctt gccacttttg
    12121 gccagatttt tcagcttttc gattcctttc cggcgtaatc ttactttcaa ttggtccaaa
    12181 aactcaccct tccttctctc ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac
    12241 tttcaaataa atgcataaat agaataaaaa gaagacctag aaatgattgg ccttcctttt
    12301 ttaatgaatt tcaactgttc agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat
    12361 atggcgcacc aaaaagggca atcaaaattc ctgtaggtac ttccccactt ggtagaatca
    12421 ctctagcaac catatctgca ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa
    12481 aggtccagtt attttcttta cgtacgacta gcttcgcagc atgaggagct agtagtccta
    12541 caaaagataa actacctgtc accgctacac taccgcttgc caaagcaaca gctaacaaca
    12601 aaaggatttt ttgactttta gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa
    12661 atgatagcaa tacaatcctc tgtgcataaa gtcctcctag cggaattaag ataaccgccc
    12721 atggaagcaa tgcccaaaca taaggccagc tcgcacccca gattgatccc gctaaccatg
    12781 cattcacaaa tccataattt tccttagata attggatcgt gcatagcaat gttaatgcag
    12841 aaaggccggc attaacggca ataccggaca ataagattcg attcataccc aaaaagccat
    12901 ttcgatcata ggccgcaaga tagacacagc aagcagctaa aacaccacct agaaaggcta
    12961 caactagcaa tagaaacggc agcgaatagt gcgaaaagaa acctaaataa agcatcacta
    13021 aaaatcctgc tccggcatta atgcccaata tactagaatc tgccaattca tttcttgtta
    13081 ctccttgcaa gatataacca gaaacgccaa gacaagctcc tgctaataaa ctgatcagta
    13141 atcgaggaac tcgaaattct ctaacaatca gcgccaaaga agatgattct ttccctatga
    13201 acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc aatcattaaa ctacctatag
    13261 acaggatgac aagtacaaaa agcaagaaat agaacgtcca atatattttt ttcaccgttg
    13321 ttccctcctt acttgtatca gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa
    13381 aggtgtttca aaagggggat tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc
    13441 accgccaact aaaaaagaga caggtagaac ataacgatag tccgttccaa ccattccgcg
    13501 aacaactggc ggaaccaaca gcccaataaa tgaaacaggt ccgactaacg aaacggcaat
    13561 ggctgctaac actgcaacta gacagaaaac aatcaaccgc gttcgtttag gatgacttcc
    13621 aagagctaat gcatgtgttt cactcaaatg aatcaaagat attgcttttc ctaatgaaaa
    13681 agaaaccaaa acagcaagta tatataaagg aaacaatact gctaattgtt tccatgtgat
    13741 attggcagct ccgcctacaa accaaaaagc aagatcctgc tgcagattaa agaattgggt
    13801 aagtgcttgg ctgatagcag taaaaaaaga actaatagct acgccagcta aaatcagctg
    13861 taaaggattc atgcccattt tcgaaaactg caaaaaacca aatacgagca aagttacagc
    13921 aaaagaacct gccaatgaca ttagaaacac agaaaaatca gaagctttag gccaaaaagc
    13981 aaaactaagg gccattgcca acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc
    14041 taaaggattt cttgtcaccc cttgcatcaa tgcaccgctc aatgcaaaac aactccctac
    14101 aagaaatgct cccgccacac gaggcaaacg aatatttcgt atgatttggt gactttgttt
    14161 tgatctatca aaatcaaaca acgcctgcca aaaatctgct ttttccacag tcgctgcgcc
    14221 atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct
    14281 atttttcttc atagtcctca taattgatcc tcctgctctt ttggcaacaa atcataggtt
    14341 agaaaaactg gtttatttgt tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt
    14401 tttttcatac actcttttgt gatcacttct tcaggtgtac cttctgtgat caacttaccg
    14461 tttttcattg ctaataaata atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac
    14521 atcaagatcg ttgtttgttt catttgattg atttcagtca ataactgtag gatctctaat
    14581 tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga tcaagatatc tgtttcctga
    14641 gcaagcgcca tagcgatcca gacacgctgg gtttgtccac cagacagcgt tgcgatatcc
    14701 cgcatacgaa atccagctag acctgtagca tccaacgccc actcaattgc ttcatgatct
    14761 tgcgtgtcca tgccactgaa cagtttttga taagggtatc gtccgtaaga tactacttct
    14821 tctactgtca tacctgtagg gtgttcacta gtttgtgcta ataatgcaat tttttttgca
    14881 agttcttttg aactgtatgt agagatatca gagttattga caatgatctt tcctgatttc
    14941 acaggtagta tacgggtaaa acttttcaat aaagtagatt ttccacttcc atttggccca
    15001 atcaaactag tgattttccc ttcaggaatc gtaacagaca tgttttcaat gatttttttg
    15061 ttttcgtagc cggctgcaat gtcagctgct cttaatctcg tcatttcacc catcctttcg
    15121 ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc gtcaagcgct actttcatat
    15181 ctatagccaa atgaagcggt tgacagcgtt cgaaatatca ggaataataa ccaatgagaa
    15241 agattctcaa ttatgttagg aggaaagaaa tgaaattaaa aacaaaagta ttcacattat
    15301 ctagcatatt gctgctggct ggagtactga gcgcttgctc cccctcttca gataaagaaa
    15361 cagctacaac tgccaattcg acgacttcat ccaatagtac tcatacagtc acagatacac
    15421 ttggacataa agtagagatt ccaaacgcgc ccaaacggat catcggcagt tacttagaag
    15481 actatctgat tgcactaggg gaaaaaccta tcgcccagtg gactgttggc agtggttcca
    15541 ttcaagatta tctgcaagat gatttaaagg atgtcccaac gatttcttat gaccttcctt
    15601 atgagaaagt gttaagcttt gagcctgatt tgctgttgat ttcttcttcc gctactgtcg
    15661 aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa
    15721 cagatgtcac atgggaagaa caactaaaag atgtcggcaa agctttggca aaagaaaaag
    15781 aggcagaaaa aatcatttcc gattatcaaa aatccatcaa gaaaactcga gaagaactag
    15841 cagataaaat cgagaacaag acagctgcag tgttgtgggt gaccaacaac tcagccttta
    15901 tggtcagcaa aaatcggtca agtggaagaa tcgtatacga cgatctggaa tttggtgttc
    15961 ctgatctagt ggaagaggtc tccaaaaaag ccacttctga ctggtctgct gtttcctctg
    16021 aaaagctagc tgaattagat gctgactaca tcatccttgt gaacagtgac aaagatgctg
    16081 ccatgttcaa tgaaccaagc tggaagaatc taaaagcagt gacagacaat catgtactgc
    16141 aattcggacc tgaatctagt tggctatata acggacctat tgccagtcag gaaatggtca
    16201 atgatatcaa agaaaaacta aattgatttt aactaaaaaa agcatcttca cgattagtcg
    16261 tgagatgctt tttttcttat taacaaacta gacgcataga ttttattctt cttttgaagc
    16321 aaccctattt tttactcttt cccatttttg aaaacgatgg gcatatagat tttcttgatc
    16381 cgttatcccc tcatactcct ctataagggc gaagtctgaa aaatcaatat tcccaatata
    16441 cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag atccgaccag tgtaaggaag
    16501 aagttcttga aaaatccgac taccacccga aacatagata tcttctgaga tcgtttgact
    16561 atacgacaaa agttcatcga tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt
    16621 tggttgcgtt gtcaacacga tgatatggcg ataaggaagt gaaagatccc ccattccttc
    16681 ataggtcttt cttcccatca ctaaaattcg atccattgta tgttcgcgaa agaaacgcat
    16741 atcatttggc aatcgccatg gtaacaaacc atttttgccg atcagaccat ttttatcttg
    16801 tgcccacatg gaaataaaca tattatccgc tccttatgat tttagatgtt ttgagataaa
    16861 aaaagtctca attctttgac cagcaaggaa ttgagaactt taaaaacatt attttgtttc
    16921 acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg
    16981 attgttacgt ttgtttttgc ttgttagata gttacgttct ttacaagaag tacactctaa
    17041 agtgatgttt acgcgcatgt ttttccctcc catttttaca agaatcctct aagatgactc
    17101 tcagacttga ttatcttacc atgatttttt ctagattgct agtgtttttc acgattttgt
    17161 taagtaaaac tacttgacga taaatacaac ggttttactt ctcaaacaaa atctttttct
    17221 ttttgtataa atacccttac acttgtttat atagattatg ttttttaata aagggggata
    17281 ctaatatgcc atggaacatg aaggactttc ctgcatcaat gaaaaactta gataaattga
    17341 caagaaaaaa agcaatcgac atcgcaaatg cattattaga tgaagggtac cctgataacc
    17401 gtgcgatccc aattgccatt gatcaagcaa aaaaatggta tgaagatgct agtgaatcag
    17461 aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac ggatgaacac gatacgaatc
    17521 ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga atatgaggaa gaacaatgga
    17581 tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt tgatacaaaa aaagaagcaa
    17641 tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac tttgttcatt tataaaaaag
    17701 atgggacaaa agaaaaagaa atcgattatt gaacatatgt aaaggggttg tgacgaaaat
    17761 cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct ctgccaactc cgaaccataa
    17821 gtcataatat ctataaaacg ggaagagctg ttttttgaaa ttgtttctta tcactcagtg
    17881 ttaaacggta ggagaaaacc tcttttttat tgatatttga ttttcatcaa acagtcacat
    17941 agtcgattgc ttctgcttgc ggaatcgttt catctacaaa gtagacttta taccatgtca
    18001 agaaactgaa gattgtccag atttttcttt gctcttcttt acgtccttcg tgatggtcat
    18061 ctagcaactt catgattttt tcttgatcaa aaaattcttt tgcaaaatct gcttcgaata
    18121 atgctttgac ttgttcgtag ccgtgttctt ctttcaacca agctttgatt ggcacaggga
    18181 atcctaattt cacacgattc gaccattctt ccggaagatg tttgttcgca gcttgtctga
    18241 aaatatcttt tgtgttgttt tgattcaata aaaacttact tggtatttgt tgagccaatt
    18301 tcatgacttc gatatccaat aaaggtactc ggacttccaa agaacttgcc atagaaagtt
    18361 tatctgcctt taacaagata tcttttggca tccattgatg aagatcgaca tattgcatct
    18421 tattgacttc atctttgatt ccttctgttt tctcgtaatg aacagtcatg atttcatcca
    18481 cagatggtgc tgtttgatat tttggtgtta acacttctga tgcttctgat tcttcgaaca
    18541 ctcgagcatg accgataaaa tattcttttg caggagcaag tgattcatat aaatggatcc
    18601 gtccatggaa gtttttcatt tttccgattt tacgtcctaa attatagcgt gttccttttg
    18661 gtaatttttt caatccttga gcaatcacac ggatgaattt tgaattcgta tggaatccat
    18721 acgtttggta tcccgcaaac agttcatccg ccccttctcc agattggact acccgcacac
    18781 tttgtgaagc aagatttgct aaaaagtaca aaggtacgca agaaggattt gaatcaggtt
    18841 catctaagtg atactggatc aaagggaatg ctttgaacga catgtctccg tcgatcactg
    18901 ctgctgtatt atctaagtcc aatagttcgg ttagttttct ggcttcaatc gcttcattat
    18961 aggttttatc atcaaacccg attgagaaag aatgatctgg tttcaataca gaggttacat
    19021 aactggaatc aaccccgctg gataagaaag aaccaacttc tacatcactg accgtatgtg
    19081 cttcaataga agcttgaacg gtttcgtcga ttttttcgat ccattcttgt cgggaatgcg
    19141 tttcttttgt ttcaaaatct gcatcccaat attgctgaat gtctattttt ccatctttat
    19201 acgtataata atggccttca ggcaaacgat agacatcttt gaagaatgtt tcgccgttta
    19261 atggagaata ttggaaagtc atataaggtt tcaacgcttc tttgttcaat tctttgttaa
    19321 aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa cataaatgtt ccgttcattt
    19381 ctgcataata atatggtttg atcccaaagt ggtctcttgc accgaataac tcattctttt
    19441 cattatccca aatagcaaaa gcaaacattc cgcggatctt ttgcaataat tctgttcccc
    19501 attcttcgta accatgcaaa aggacttctg tatctgcatg tgtttgaaaa acgtggccag
    19561 ctgcaatcaa ttcttctctc aaaggctggt aattatagat ttcgccatta aaaataatga
    19621 tctttgtccg atcttcattg taaataggct gagtaccgcc ctctaagtca ataatactta
    19681 atcttctgaa tcctaaagca acatgtttat cgatatattc tcctgaacta ttaggtccac
    19741 ggtgaacgat tcgatccatc attgtattaa taattgtttt tttgttgtcc ttgtcgttaa
    19801 caaaacctac aataccgcac atttaacttt cacatcctaa tataaaatta ctgtttattt
    19861 cattcatatg catcttatca tagaataaat agcggacgaa tattttctct cttacaattt
    19921 cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca gaaaataaag tcccaatatc
    19981 taatttacca tgttttcttt tttgaggcta ccttattctc ttcgtgttaa aaaaatatta
    20041 gtggatgcta tttccaaaaa aaccagcaaa aaaagacacg acctaaagat cgtgt
//
LOCUS       DANJLF010000045        19821 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000045 DANJLF010000000
VERSION     DANJLF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            6..305
                     /locus_tag="PWT25_001813"
     CDS             6..305
                     /locus_tag="PWT25_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1021901.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PWT25_001814"
     CDS             378..1739
                     /locus_tag="PWT25_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL1021902.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PWT25_001815"
     CDS             1761..2021
                     /locus_tag="PWT25_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021903.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PWT25_001816"
     CDS             2035..2787
                     /locus_tag="PWT25_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL1021904.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PWT25_001817"
     CDS             complement(2881..3486)
                     /locus_tag="PWT25_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1021905.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PWT25_001818"
     CDS             complement(3539..4513)
                     /locus_tag="PWT25_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL1021906.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PWT25_001819"
     CDS             complement(4891..5610)
                     /locus_tag="PWT25_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1021907.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWT25_001820"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWT25_001820"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL1021908.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PWT25_001821"
     CDS             7161..8558
                     /locus_tag="PWT25_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1021909.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PWT25_001822"
     CDS             8639..9079
                     /locus_tag="PWT25_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1021910.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PWT25_001823"
     CDS             9227..9553
                     /locus_tag="PWT25_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1021911.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PWT25_001824"
     CDS             9554..10303
                     /locus_tag="PWT25_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021912.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PWT25_001825"
     CDS             complement(10383..10838)
                     /locus_tag="PWT25_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL1021913.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="PWT25_001826"
     CDS             complement(11265..11540)
                     /locus_tag="PWT25_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021914.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="PWT25_001827"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="PWT25_001827"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL1021915.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PWT25_001828"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PWT25_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL1021916.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="PWT25_001829"
     CDS             14770..17436
                     /locus_tag="PWT25_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1021917.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="PWT25_001830"
     CDS             17800..18552
                     /locus_tag="PWT25_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1021918.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="PWT25_001831"
     CDS             complement(18574..19728)
                     /locus_tag="PWT25_001831"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL1021919.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6329 a         3472 c         4060 g         5960 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa atggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DANJLF010000046        19376 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000046 DANJLF010000000
VERSION     DANJLF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            278..2749
                     /locus_tag="PWT25_001832"
     CDS             278..2749
                     /locus_tag="PWT25_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1021920.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2852..4588)
                     /locus_tag="PWT25_001833"
     CDS             complement(2852..4588)
                     /locus_tag="PWT25_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL1021921.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4585..5289)
                     /locus_tag="PWT25_001834"
     CDS             complement(4585..5289)
                     /locus_tag="PWT25_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1021922.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5420..5923
                     /locus_tag="PWT25_001835"
     CDS             5420..5923
                     /locus_tag="PWT25_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL1021923.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5883..6221
                     /locus_tag="PWT25_001836"
     CDS             5883..6221
                     /locus_tag="PWT25_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL1021924.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6242..7660
                     /gene="ffh"
                     /locus_tag="PWT25_001837"
     CDS             6242..7660
                     /gene="ffh"
                     /locus_tag="PWT25_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL1021925.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7772..8350
                     /locus_tag="PWT25_001838"
     CDS             7772..8350
                     /locus_tag="PWT25_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL1021926.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8354..8875
                     /locus_tag="PWT25_001839"
     CDS             8354..8875
                     /locus_tag="PWT25_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL1021927.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8954..9508)
                     /locus_tag="PWT25_001840"
     CDS             complement(8954..9508)
                     /locus_tag="PWT25_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1021928.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9761..10036
                     /gene="rpsP"
                     /locus_tag="PWT25_001841"
     CDS             9761..10036
                     /gene="rpsP"
                     /locus_tag="PWT25_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL1021929.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10048..10299
                     /locus_tag="PWT25_001842"
     CDS             10048..10299
                     /locus_tag="PWT25_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL1021930.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10424..11215
                     /locus_tag="PWT25_001843"
     CDS             10424..11215
                     /locus_tag="PWT25_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021931.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11385..11906
                     /gene="rimM"
                     /locus_tag="PWT25_001844"
     CDS             11385..11906
                     /gene="rimM"
                     /locus_tag="PWT25_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL1021932.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11896..12657
                     /gene="trmD"
                     /locus_tag="PWT25_001845"
     CDS             11896..12657
                     /gene="trmD"
                     /locus_tag="PWT25_001845"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL1021933.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12793..13140
                     /gene="rplS"
                     /locus_tag="PWT25_001846"
     CDS             12793..13140
                     /gene="rplS"
                     /locus_tag="PWT25_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL1021934.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13272..14408)
                     /locus_tag="PWT25_001847"
     CDS             complement(13272..14408)
                     /locus_tag="PWT25_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1021935.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14524..15438)
                     /locus_tag="PWT25_001848"
     CDS             complement(14524..15438)
                     /locus_tag="PWT25_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL1021936.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15529..15957)
                     /locus_tag="PWT25_001849"
     CDS             complement(15529..15957)
                     /locus_tag="PWT25_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL1021937.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15962..16336)
                     /locus_tag="PWT25_001850"
     CDS             complement(15962..16336)
                     /locus_tag="PWT25_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1021938.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16622..16807
                     /locus_tag="PWT25_001851"
     CDS             16622..16807
                     /locus_tag="PWT25_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1021939.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16819..17067
                     /locus_tag="PWT25_001852"
     CDS             16819..17067
                     /locus_tag="PWT25_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021940.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17098..17277
                     /locus_tag="PWT25_001853"
     CDS             17098..17277
                     /locus_tag="PWT25_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021941.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17320..17790)
                     /locus_tag="PWT25_001854"
     CDS             complement(17320..17790)
                     /locus_tag="PWT25_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021942.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17877..18575
                     /locus_tag="PWT25_001855"
     CDS             17877..18575
                     /locus_tag="PWT25_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL1021943.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18753..19094
                     /locus_tag="PWT25_001856"
     CDS             18753..19094
                     /locus_tag="PWT25_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021944.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19087..>19376
                     /locus_tag="PWT25_001857"
     CDS             19087..>19376
                     /locus_tag="PWT25_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL1021945.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6378 a         3385 c         3816 g         5797 t
ORIGIN      
        1 tccattgaga gactcctttg gtaggatgac gttttggtcg actaaatttt accaaaagtg
       61 agtctctttt tgagttgaaa cgaagaaaaa gtgttagtag aaaatcgcct tcgattttcc
      121 accaacacaa aatattatag aacccttttt ttttgctata tgacaggaat gaaaggtgtt
      181 tgcaagctta atgtgaggaa cactttccat atctgttcta tactggatag gagaagtttg
      241 gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg aagaataaat ggataaatcg
      301 aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa gtcatccgtc tttttccatc
      361 gtataaagaa ggattaacgg cagatcaagt caaggaacga atagaaaaag gtgcagcaaa
      421 taattcagtc gatcccacat tcaaaacaaa tcagcaaatc gtattggaaa acatatttac
      481 gtatttcaat ttgatctttc ttatcttagc cattttgctt tgtttagtag aatcctataa
      541 gaacttgact tttttaccag ttatcatagc caatacagga atcgccattt ttcaggaaat
      601 tcgatccaaa aagatcttgg acgaactaaa tgttttaaat acagcaaaag tcaatgtcat
      661 acgagacggg atagaacaaa caatagatat agaagacctt gtaatagatg acattgttat
      721 tttagagact ggatatcaaa ttccagcaga tgcagaggtc gtggaaggga aactgcaagt
      781 taatgaggct ttattgacag gcgaagcaga tgaaataacc aaagaaatag gtgatccatt
      841 aatgtccggc agtttcattg tttccggaaa agcttacgcc cgattagaca aagttggagc
      901 agattcgtat atttcccagc tgacagtcaa agccaagaca atgggagaag gagaacagtc
      961 ggagatgatt gcttctctta atcaattgat caaatggatt gggatcatca ttattcctat
     1021 cggtatcctg cttttttctc aaagttactt ttttaatgga aacacgatca aagaaagtgt
     1081 cgttgcaatg gaagcagcat tgatcggtat gatacctgaa ggactgtact tgctgacgac
     1141 gattgcatta gcattaagtg cgactaaact agcaaagcaa agagttcttt tacacaatat
     1201 gaaaagcatt gagacattgg caagggtaaa tgtcctttgt gtagataaaa caggaacgat
     1261 cacagaaaat aaaatgtctg ttcaaaaaat gatcgtctct aaaaaacaag aaataatcag
     1321 tcagtcagat caattggaag catgcattgc tgactatgca aaagcgatgg caggagacaa
     1381 tgcaacaatg gaagctgtta aagcatattt tacaaaaacc actgatcaat cctatcacca
     1441 tgttattcct ttttcttctg tccaaaaatt cagcagtgta gcgttggcag acaaggtgta
     1501 tgttttagga gcgcccgaaa tggttttgcg cgatcaattg ccggaatatg cggaagagtt
     1561 tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt ggtgtgtacg atgggattct
     1621 tgaagaacca gtattgacag aagcagtcac accattaggc tatattctga ttgctaatcc
     1681 tatcagaaaa gaagcaagag ccacatttga ttacttcaaa aaacagcaag tagccatcaa
     1741 agtgatttct ggagataatc cgctcaccgt atccaatgta gctgtccaag cagggatttc
     1801 acatgctgat cagtacgttg atgcatctaa attatcagaa gaagaatatg aagcagctgt
     1861 cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa aagaaaatct tcgttcaatt
     1921 gataaagcgt aacaatacag tagctatgac aggtgatggc gtgaatgata ttctagccat
     1981 gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca tgcaggcctc
     2041 acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg cagaaggacg
     2101 gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga atattttctc
     2161 ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag aaccatcaca
     2221 aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg ctctagaaga
     2281 aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag ctgtgccagg
     2341 tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga ctttgaatct
     2401 aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag ggttcctcgt
     2461 tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc ttttctgtgc
     2521 tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca ccggtatctc
     2581 agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga tttttaggca
     2641 tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc aaaagccgtt
     2701 tagtcgcgta gctaaaaaat ttctatcgat atttaaacga gaaaattgac gaaaagtatc
     2761 tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga tgagtcatcc
     2821 aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta acggtgaaag
     2881 tcgaacctgt tccaagatga ctgtctacag taatcgtacc attaaggagt tgcacgtagt
     2941 cttttacaat cgctaaccct aatccggtcc cgcctgaatg tcggcttcgc gccttgtcta
     3001 cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct ataccaaagt
     3061 cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc tcgctgtcgc
     3121 tttttgaata ttggacagca ttttcaatta gatttttgat aattggataa aggatatctg
     3181 ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga
     3241 tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa attgtctgga
     3301 tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc agtttatcta
     3361 accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc gggtcatctt
     3421 ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga gtttttaatt
     3481 cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc actttcgtta
     3541 agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag tagcgcaaac
     3601 tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc agcggatgct
     3661 caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact tctgtgtacc
     3721 gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga ttaagcagta
     3781 gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg ttcagtaacg
     3841 tatataattg ctcttctgtg gtagtacacg cccgatacat cttactcatt tgttcgctca
     3901 acgcattaat cgtctgatac aattcttccc attgatcggg tgtttgcatg attacttccg
     3961 cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc aatggctggt
     4021 ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa gacagaaaga
     4081 agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt gtcggttctg
     4141 cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg acatataaaa
     4201 gatcctcatt caaagtagta cttttgcgca aagcgctacc taggttccca ccattcaata
     4261 cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa
     4321 ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga tggacgaatt
     4381 cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat tgccggatca
     4441 gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc tgttgaaaat
     4501 aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata agaaagaaga
     4561 gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat agccaaatcc
     4621 tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt ctcgcaaatg
     4681 actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc
     4741 tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa caagtaattc
     4801 aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat aattggtcaa
     4861 atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt ttgattgctt
     4921 acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc gaggactaaa
     4981 tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat ccacttggtc
     5041 atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca gccgctgagt
     5101 gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat caaatgattg
     5161 ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct tgtagccttc
     5221 tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa caactagtat
     5281 ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta gcatagaatc
     5341 agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca ctttgctgaa
     5401 tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg
     5461 ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa ctggagaagc
     5521 tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc ccgctgaaat
     5581 cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga cgacaagcgg
     5641 cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa aattgcagga
     5701 aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa cccattcgat
     5761 ttatgacgga agtttttccg gaataaaaaa aaatggtgcc ggagtggcta ctgcttattt
     5821 tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta gaggtgagca
     5881 gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat tcgaccttat
     5941 tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt tcattaggtg
     6001 agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc aagcggacaa
     6061 gcaagatctt ggaggattat gaaaaaaaac tccatctttt ttctaattat attgttcgag
     6121 aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa gataaaaaac
     6181 tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga atgaagaaaa
     6241 tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa taagaaaaaa
     6301 aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc ttgccttact
     6361 agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac gcgaacgtgc
     6421 ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa aaatcgttga
     6481 tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat caccaaaaat
     6541 accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct ttactggtaa
     6601 attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg caggggacgt
     6661 atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag acgttcctgt
     6721 atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga tggagttagc
     6781 aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc atatcgatga
     6841 aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg agattttgct
     6901 tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct tcaaccagca
     6961 attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag gcggggcagc
     7021 actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg gtgaaaaatt
     7081 gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg gtatggggga
     7141 tatgctgaca ttgattgaaa aagcgcagca agactacgac gagaaaaaag cggaagaact
     7201 tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac agcttgatca
     7261 agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa tgaatcaagt
     7321 ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa aagcgatggt
     7381 ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa gccgtcgtcg
     7441 ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga tcaaacaatt
     7501 caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca tcccaggtat
     7561 ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga tgaatcgaat
     7621 gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa acagcttcgg
     7681 tacagactga aaatgtctac tgccggagct tttctatcag caatttttat tctttactaa
     7741 tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg aacaaacaga
     7801 aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg accaaaagct
     7861 gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga cgattttaaa
     7921 taaaagtgaa gcattcgatg aagcatatga tcgattcgat atcaaaaaag tcgcaaatta
     7981 tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta accgaagaaa
     8041 aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag aaatcggcag
     8101 tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt cttatcattc
     8161 acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag atctaaagaa
     8221 aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag ctgtcgggat
     8281 catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta tcggcagaaa
     8341 gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag atggctattt
     8401 ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta agcaaactac
     8461 ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa cttatcagga
     8521 agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt tcgttttttc
     8581 cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt
     8641 tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt
     8701 gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga ttacccctgt
     8761 attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc gttttgaaat
     8821 gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa agtagaagta
     8881 aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc cttttttatt
     8941 accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca tctaaaatcg
     9001 tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt agattcgcaa
     9061 cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag acaagtgatt
     9121 ggcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca gtaacgacta
     9181 aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca tctaaagcat
     9241 ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg acaccgtcta
     9301 gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct ggagtttcga
     9361 gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg tacccttcag
     9421 gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt cttaaactac
     9481 caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact atctataata
     9541 atcttacaaa agataagtaa ctggagcaaa taaaaacact tactgacaaa agctacatag
     9601 aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa
     9661 atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca
     9721 ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta aaatccgttt
     9781 aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg attcacgttc
     9841 tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga aagaccctgc
     9901 agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg cacaaccttc
     9961 tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc acgaagctaa
    10021 attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt cttaacaatc
    10081 gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac
    10141 ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat tggaaaacaa
    10201 gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa
    10261 aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct gtactcgaat
    10321 tttcgagtgc agcttttttg ttttttttat taattgaaga aatgattatt tggcaaaaca
    10381 agcaagaaag cgtatcctta taggaggagg gagtgaacag ccaatgagaa agaaaatgtt
    10441 atttattcca tttgctctat tgttaagcgc ttgtagttcg gttactcaag aatcagacaa
    10501 tacacatcta tcaagcagta tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa
    10561 aacggcttcc accactgaac aaacaatggc tacctcaaat aatgagaaaa agactgcttt
    10621 agaccagttg aaagaacaac agccaaatgt tcccatgcct ctagatgtac cagtctcttc
    10681 aggctatctc aatatcgcag cgacacatac aaagcaaggt tattctattt tatattacag
    10741 aacagatcgt cccctcgggt tgaatgctga cgaattgaat caagaaacgc ctattgcaac
    10801 ctatctatat caatatgggt ttgcttctag tcaagaaaca atccaagttt tgcagccatt
    10861 tgagatcgac acaaatggtc agcaagtgga cttaggttct cgaatcacag ggtatcagca
    10921 aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga aactggcgta ttcgaatccg
    10981 tgggaataat atagaaggcc aagatccctt actattagct aaagaaatcg tagcttattt
    11041 ggaagaaaac agtctaccag cacctgaaca atttggtaaa attactgttg atatgggtga
    11101 tacgacgaat cgagccgtag aagtaagctg gcaagaaccg aaaaatgcct atacgatcac
    11161 tcatcaagat cctatgtctg cgttgaagat ggcagtttct atgaagaggt tgtgaaatca
    11221 gcgcgctgat ctgagcccat catttaactc caagtcacag gaagtaatca aaaatagcac
    11281 gccgcatttt tgcataattt tggcttaata ccttaaaaga aaactagaat tgcctcttca
    11341 cacatgctaa aataaaacag acaaaaaaga aatggggata taagttgact gaatacttga
    11401 atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga agtccgtgtt atctcaacaa
    11461 cggatttccc agaagaacgt tacaaaaaag gaacggtgct aacactattc caagacggaa
    11521 aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca taaaaatttt gacttattga
    11581 gttttgaagg acatccttct atcaatgatg tggaaaaata tcgggatggc atattaaaag
    11641 tgtcaaaaga caactctgta gatctagctg aaaatgaatt ctattaccat gagatcatcg
    11701 gtctgcatgt attagaagat ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag
    11761 gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc tgatatattg cttccatata
    11821 tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt ggtccgtgta gaaattccag
    11881 aaggactgat tgacgatgaa gattgatgta ttgactttat ttccaagaat gtttgaaggt
    11941 cctatggggg aatccatcat cggtaaggct gtagataaag gattattaga tatcaatatc
    12001 tctaacttcc gcgattattc agataacaaa catcagacag tagatgatta tccatacggc
    12061 ggtggagcag gtatgctgct caaagtccag cccatctatg ataacatcca agccattgaa
    12121 aaagctgcac cggatgtcaa aaaaagagta attcttcttg acccagctgg taaacgattc
    12181 gatcagaaaa tggcagaaga attttcacaa gaagaacact tagtattcat ttgtggtcat
    12241 tatgagggat atgatgaacg tatccgcaca ttagtgacag atgaagtttc cttaggagat
    12301 tatgtcctta ctgggggaga attaggtgcg atggtcatga tcgatgcaac agttcggctt
    12361 ttgccagatg tgttagggaa tcagacttca gctcaaacgg attcttattc aaccggttta
    12421 ttggaacatc cccaatatac tcgcccggca gaatataaag ggatgaaagt accagaagta
    12481 ttgacaaatg gaaatcataa attgattgaa gaatggcaat taaaagaatc actaagaagg
    12541 acctatcaac gtcggcctga tcttttggaa acacttgaat ggacacctca aatgacgaaa
    12601 tttttagaag aaatcaagaa agaagaacaa gaaaaaacag cagatgaagc caagtgactt
    12661 tttctaaaat cttctttaca gtaggttgat aatatgttag tattttatgg tgagtgcaaa
    12721 agcacttaac tattacgata ttccgctgtg agaaaagctc ataagaatat ttggaataag
    12781 gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa aagaacaatt acgttctgac
    12841 atcccagcgt tccgccctgg tgacactgtg cgtgttcacg caaaagttgt tgaaggtact
    12901 cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc
    12961 gaaacttata cagtacgtaa agtttctaac ggagtgggcg ttgaacgtac attcccattg
    13021 catacaccac gtgttgcaaa aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa
    13081 ttgtactacc tacgtgcatt acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa
    13141 tcatcgttat cagactgaga aagaaccctt ggtattcaag ggttcttttt ttgttctctg
    13201 tttttttata ttaacgattt ctatttggga catttttgat taataaaaag gggcaaaaaa
    13261 ggggcaacaa gttacagttt ttctatatct tctttcaatt tttcttttac tccctttgtc
    13321 acatgcaaat aaatcttttc agtgatatca ctattttcgt gcccaacgcg atcctgaata
    13381 gcatacagag gagttcctag ttccgctaat tttgaaatat gggtatgtct aaaaatatgt
    13441 gaactaagtt ttttatcaat cttcatgtca gctttatgat ttctcaaata ggtgttaatt
    13501 gctgttagtt ggaaaggagt tcctttcgtt gtttgaaata gaaattgacc attcggattt
    13561 aattctagaa gctcattgta aatagctatc gctttttttg gtaaatcaat ttctcgcatt
    13621 ccagcagcag tttttgtaga atcagatttt ttcatatcag ctattgaacg ttctctatac
    13681 atcatcgtcc catttataac tactgatgca ttattattgg taatgtgtac atcgtcttta
    13741 cttaatgcga ttgcttctcc aggcctcatt ccagtcaaat atagccactg gaaaaggaga
    13801 gaatatcttt tattgtgtga agttgtgaaa tttactagtc ttttatattc atcatcttct
    13861 aaaaacttat ctttaatttt tatcgttttt gactctcgtt tataatcaat aactacttcg
    13921 ttgataggat ttttttctac atagcctttt ttcatagcgt atgaaaaaag aagattcaat
    13981 ttggatttaa ttacactgac gtacttattt gacaagtcat ctttatatat catatcttca
    14041 aaaatattat ttaaatctac tgtattaata ccagaaacga tgtaagtctc aggtatcttt
    14101 ttctttatag tgtttaagat attattcgta ggataatatg tagactcctt aacttgtctt
    14161 ttgtaaataa ctaaccattc ttcgacaagc tcatgaaatg ttagatcagg cttctgaagt
    14221 gttttttcgt ttaattttat atcaattttt ttatttaact ctagcaatgc cactttctga
    14281 gtttctcgag atttattttt ataagtaatg ctcacttttt ttctttttct agtttttgga
    14341 tcaatatatc gctcattata tttatatact ttttttccgt ttttatcagt tttggtttca
    14401 atccacattg ttttcatctc ctatttgttg ctataatagg aatggataag taagccaatt
    14461 atagcaggtt tatttttcat cacgtccaca aactttggcg agggaggggg cgtgtttttt
    14521 tgtttagtag ctacctttcc atgaagtatc tgcgaagaac acttcaaatg ttgttccatc
    14581 atttttcact tcaaaatatg ctgttccttg tgcggattta cccggttgga tggtttccga
    14641 aaagtaatct ctactagcta aatcagattt aatatctgat gaatcataaa attctaaata
    14701 acttgaactt gcatcaaaag gtgtagtccc tgtgttttct acaagaaaat ctacctttgc
    14761 gtaaaatagg ccttctggtt tatagaaatc atcaccgtaa tcttttgata cagactggac
    14821 agttacgtta atagagttac cattttcaga agtaaaagga actgcttctc ctatactatg
    14881 tgcaactgat gccgtctggg gttctgaaga cgaagttgta gttgtagttg tagtagagct
    14941 tgaatctttg gattttccat ctttatctaa gaaagttcgt tgattataat atacaacctg
    15001 ttcagttttt ggactaaccc aaattaacat tgtttcatca tcagaatcag ttgaataagt
    15061 atattggagc atctctaatt tttttatatc ttttgcacta tttccatgtt caattaattc
    15121 agagtgctct ttataataat ctccttgtgc atcctttgaa atagatacaa ttaattcatc
    15181 ccgtgtgata gcattgtcta attctttgta aacaaaatca tttgtccatt tacgtaacgg
    15241 tgaaccaagt gtctgataaa cttgagttaa atcatcacca acttttattt tatgaacgtc
    15301 tgatgaagtt agtttttttg tttcatattc agatgttgat gaaacagtat tttctttagt
    15361 gctagcagaa ttaggactag tggagcatcc gcttaatacc aagcctaaaa aaagcaaccc
    15421 aaaaacaatt tttttcattt ttaattcctc acttctatga tatgattttt ttgtaggatc
    15481 ttagaaataa ggtttttagt ccgtgttgca gcacggactt tttttacatt atataagaaa
    15541 gcgataagcg ctttctggaa gtccgtaaag attctttaat tcctcaattc gtttaggata
    15601 ttgatcattg tcttctttgt atagagaaac aatgagatta gcagcaaagc aattagcttc
    15661 gctttcagat ttgcttctag atgttcttgt tgatacatag taactggata agccacgatg
    15721 aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat ctttcctcag agtctctcag
    15781 ttcatcattt aagaagatta ttgcacgacc tctaatttct tgaaactgtc ctttgggatt
    15841 ctcgataaaa ggaacgtatt gaattttaat gcccatcttt tcacaaatat aaaaaggatt
    15901 agcggactgg tatttccgct tcaactcctc gactaaatta atcgtatcca tctccataag
    15961 ctcacatctt tttgcctttt tctttgtctt ctttcacaat atcccagaag gttccaataa
    16021 ggatatcttt tacgcgttgg acctgttcag gcgtcagtgt ttctccgcca taagacatat
    16081 tgacatttga atctagtaat ttatcaagtt caactacttc atcttttgtt gcccagtcag
    16141 gaacttggtt tctaccgagt aaataatcag tagttacatt aaaataatct gctacaagtt
    16201 gtagtctttt agtactagga gttctatttt tccattgata aatcgtgttt ttaggtatat
    16261 ttaattcttc ttccaattga gttatactta tgcctctttt atgagctaat tcctttattc
    16321 tatctagtaa attcattttc gcacctcaca agctacgaaa acactaataa aaaaattagt
    16381 taaaggtgtt gacaactaat aaaagtgtta gtatactgtt ttcgtaagct aaaatattag
    16441 ctaaaaagac ttgataacta ataaacactt cacggtcggc aaactctgaa atgtaaatta
    16501 ctaggcattt ctgtgtctta tttagctatg tctatatact aataaaaata ttagttattg
    16561 tcaacgaata ttagctaatt ttttagctta cagattattt ttttagaaag gagctatttt
    16621 tatgtctgag aatttagact taaaaattcg agcggagatg agaaaaagaa gaatgacttt
    16681 caaagaacta gctgcgctta tcggtatttc aggagcttat ttatcagata ttctaaacgg
    16741 caatcgtgat ggaaagaaag cacaacagca tatcgaaaca gtgaaaaaaa tattggacat
    16801 ccgatagggg ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa aaaatacaat
    16861 tattctccat ctacttatca acgtcgaatg tcggaactaa aaaatacagc aattttctca
    16921 gcggcgtatg aacgggtcac aggacaagaa gtttggatca atacagaatt atacgataaa
    16981 tttttgtctt tcaaatccta taacaggtta cgcacaagaa aggtaacacc taaagaattt
    17041 atcgagaagc atttagttga tttataaaaa acagaacatt ttgagaggtg aaggttaatg
    17101 ggtaaattca acagagcatt ggtattcagc gcaccgctaa tcatctacgc tttaggactt
    17161 tggggaagca ggcaagcgtt gataggaacg attgtttaca tggtttggat ttttatgggg
    17221 cttgatgaag ctgaagctga gtacagagcg aaaaagccaa ccgagggggc tgactaaaaa
    17281 tgaatagaaa agagaaacta gaatgtatat tactattact cagtttaatt ctttcactaa
    17341 tttctctatt gggaagtttg tatttttgat atcaacaatt ttgtggattt gggttctatt
    17401 tagagtaaat atcatttttg tttggtgatt aaggagttta ttaaaattaa ttttccctac
    17461 ttcaaatgca aggtaacaag atatagcact tttaggagga atatttagcg gtacagcttc
    17521 agagtatatt ctattatcat cgactgatcc tcctttagca attaatttct taaatggagt
    17581 agctgaccat ctgcggttta agttagcgga tgaaagttct aaattaacta acatagctgg
    17641 ttctgttgaa tagttagaaa tgattacttt tgtataaatc atatctgcag cgaagtaaga
    17701 agcattaagt tcaacttcta gttgagggcg cattcctttt atttttatcc ctgttaaaaa
    17761 cgtactcaat ataaaactta ttattgacat ccattgaaaa attgttaagt gaaaaaaatt
    17821 caaaataaac accaccagtt tttaactaaa ttataccaaa aaggagagaa gaaataatgc
    17881 aagaattagt aattttgaaa aataaagaag ctgtgactac gagcttacaa gtggcagaaa
    17941 gcttcgagaa aaaacatcaa catgttttaa gagatattga tgcactaaaa aaagatgtgt
    18001 ccaattttgg acagatgttt gtagaaggta atgaaccaga ttcatatggc agaaatcgac
    18061 gagttttctt cattagtaga gatggatttt tcttgctggc tatggggttt acaggaaaga
    18121 aagctatcta cttcaaacaa aaatacattg aagcattcaa cgaaatggaa gatgttattc
    18181 gcaagaatac tgttcctcaa acaattgaag atatgatgat ctatcaatta gaagaaatga
    18241 aagatgttaa aaaagatgtt tccatgctta aagatactat gcgaattagc ggacaacaag
    18301 agtttgaaat taagcaaaaa ggaaatatga aagttatggg agttctaggg ggaaaagaaa
    18361 gccgagctta tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac
    18421 gtaccttttc aatcccaaga tatggcgagt tacctcgtaa gagattcgat gatgctgttt
    18481 catttattga aatgtggttg ccagaaactg cgatccgtat ggaaattgat caactgaaca
    18541 gacaacagag actttttggt gatgacaatg aatagagctg aagcgctaag aatagggacg
    18601 gcaattgcta attgctggtg gaaatactat aaaccaatca tcctaagcca acaacatatt
    18661 gacaagcaaa aagcatggca acaaataaaa aagtgactcc gccggcaagc aaagagtcac
    18721 aaagaaaaca catcataagg agattttagc atatggaaaa agaactttcc actctagatc
    18781 aatatttgat tgatcctgat tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa
    18841 tcagacgaaa tcttttgacg aatgaagaac tagcttggga tcaagatgat ttaggcaacc
    18901 atgtaactat ttgggatcat gtttatctta tccatctatc gaagcattcg aataaacctg
    18961 aatatattta cgtcatcgaa gatggcttga ttgatgcgct agaagagtac gacagagata
    19021 acttgattga tatctcttat tacggaccag gtaagaaata cattgctgaa atggaggcag
    19081 aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag tcgtaagtta
    19141 aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca cgcgtgggag
    19201 attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtacaa acactacagg
    19261 gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt tcttatggat
    19321 gaaactggga cttatgtatc tgtctcagta acggttaaag gacacacgga aaccga
//
LOCUS       DANJLF010000047        19200 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000047 DANJLF010000000
VERSION     DANJLF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            102..2543
                     /locus_tag="PWT25_001858"
     CDS             102..2543
                     /locus_tag="PWT25_001858"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL1021946.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="PWT25_001859"
     CDS             complement(2873..3577)
                     /locus_tag="PWT25_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021947.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="PWT25_001860"
     CDS             complement(3764..4009)
                     /locus_tag="PWT25_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021948.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="PWT25_001861"
     CDS             complement(4026..4877)
                     /locus_tag="PWT25_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1021949.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="PWT25_001862"
     CDS             5043..6047
                     /locus_tag="PWT25_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL1021950.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="PWT25_001863"
     CDS             complement(6288..6644)
                     /locus_tag="PWT25_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1021951.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="PWT25_001864"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="PWT25_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL1021952.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="PWT25_001865"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="PWT25_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL1021953.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11765)
                     /locus_tag="PWT25_001866"
     CDS             complement(11004..11765)
                     /locus_tag="PWT25_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1021954.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12043..12564
                     /locus_tag="PWT25_001867"
     CDS             12043..12564
                     /locus_tag="PWT25_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL1021955.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12623..13483
                     /locus_tag="PWT25_001868"
     CDS             12623..13483
                     /locus_tag="PWT25_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL1021956.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13668..15983
                     /locus_tag="PWT25_001869"
     CDS             13668..15983
                     /locus_tag="PWT25_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1021957.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /locus_tag="PWT25_001870"
     CDS             16165..18006
                     /locus_tag="PWT25_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL1021958.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="PWT25_001871"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="PWT25_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL1021959.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6226 a         3392 c         4028 g         5554 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
//
LOCUS       DANJLF010000048        19126 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000048 DANJLF010000000
VERSION     DANJLF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..703)
                     /locus_tag="PWT25_001872"
     CDS             complement(<1..703)
                     /locus_tag="PWT25_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1021960.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="PWT25_001873"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="PWT25_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="PWT25_001874"
     CDS             1758..2837
                     /locus_tag="PWT25_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL1021961.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="PWT25_001875"
     CDS             2869..3462
                     /locus_tag="PWT25_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1021962.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="PWT25_001876"
     CDS             complement(3572..3799)
                     /locus_tag="PWT25_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1021963.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="PWT25_001877"
     CDS             complement(3902..6490)
                     /locus_tag="PWT25_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL1021964.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="PWT25_001878"
     CDS             complement(6510..7169)
                     /locus_tag="PWT25_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1021965.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="PWT25_001879"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="PWT25_001879"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL1021966.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="PWT25_001880"
     CDS             complement(7942..8808)
                     /locus_tag="PWT25_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1021967.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="PWT25_001881"
     CDS             8917..9558
                     /locus_tag="PWT25_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL1021968.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="PWT25_001882"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="PWT25_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL1021969.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="PWT25_001883"
     CDS             complement(10999..11898)
                     /locus_tag="PWT25_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL1021970.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="PWT25_001884"
     CDS             complement(11900..12697)
                     /locus_tag="PWT25_001884"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL1021971.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="PWT25_001885"
     CDS             complement(12675..13379)
                     /locus_tag="PWT25_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL1021972.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="PWT25_001886"
     CDS             13510..14085
                     /locus_tag="PWT25_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL1021973.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="PWT25_001887"
     CDS             14203..14880
                     /locus_tag="PWT25_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL1021974.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="PWT25_001888"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="PWT25_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL1021975.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="PWT25_001889"
     CDS             complement(16943..17941)
                     /locus_tag="PWT25_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL1021976.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="PWT25_001890"
     CDS             complement(18167..18820)
                     /locus_tag="PWT25_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL1021977.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DANJLF010000049        18740 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000049 DANJLF010000000
VERSION     DANJLF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            256..531
                     /locus_tag="PWT25_001891"
     CDS             256..531
                     /locus_tag="PWT25_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1021978.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="PWT25_001892"
     CDS             544..2616
                     /locus_tag="PWT25_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL1021979.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="PWT25_001893"
     CDS             2781..4667
                     /locus_tag="PWT25_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1021980.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="PWT25_001894"
     CDS             4679..5626
                     /locus_tag="PWT25_001894"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL1021981.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="PWT25_001895"
     CDS             5646..6173
                     /locus_tag="PWT25_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL1021982.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="PWT25_001896"
     CDS             complement(6236..6889)
                     /locus_tag="PWT25_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL1021983.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="PWT25_001897"
     CDS             7021..7863
                     /locus_tag="PWT25_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1021984.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="PWT25_001898"
     CDS             8021..8917
                     /locus_tag="PWT25_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL1021985.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="PWT25_001899"
     CDS             8920..9633
                     /locus_tag="PWT25_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL1021986.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="PWT25_001900"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="PWT25_001900"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL1021987.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="PWT25_001901"
     CDS             10168..10395
                     /locus_tag="PWT25_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL1021988.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="PWT25_001902"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="PWT25_001902"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL1021989.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="PWT25_001903"
     CDS             complement(11181..11681)
                     /locus_tag="PWT25_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL1021990.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="PWT25_001904"
     CDS             complement(11960..12358)
                     /locus_tag="PWT25_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1021991.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="PWT25_001905"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="PWT25_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL1021992.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="PWT25_001906"
     CDS             13471..14238
                     /locus_tag="PWT25_001906"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL1021993.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="PWT25_001907"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="PWT25_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL1021994.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="PWT25_001908"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="PWT25_001908"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL1021995.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="PWT25_001909"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="PWT25_001909"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL1021996.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DANJLF010000050        17980 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000050 DANJLF010000000
VERSION     DANJLF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(29..727)
                     /locus_tag="PWT25_001910"
     CDS             complement(29..727)
                     /locus_tag="PWT25_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL1021997.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="PWT25_001911"
     CDS             complement(720..2072)
                     /locus_tag="PWT25_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL1021998.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="PWT25_001912"
     CDS             complement(2295..3695)
                     /locus_tag="PWT25_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL1021999.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="PWT25_001913"
     CDS             3856..4593
                     /locus_tag="PWT25_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL1022000.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="PWT25_001914"
     CDS             4718..5197
                     /locus_tag="PWT25_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL1022001.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="PWT25_001915"
     CDS             5194..5961
                     /locus_tag="PWT25_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022002.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="PWT25_001916"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="PWT25_001916"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL1022003.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="PWT25_001917"
     CDS             complement(6864..7466)
                     /locus_tag="PWT25_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1022004.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="PWT25_001918"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="PWT25_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="PWT25_001919"
     CDS             8147..8731
                     /locus_tag="PWT25_001919"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL1022005.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="PWT25_001920"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="PWT25_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL1022006.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="PWT25_001921"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="PWT25_001921"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL1022007.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="PWT25_001922"
     CDS             10632..10811
                     /locus_tag="PWT25_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022008.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="PWT25_001923"
     CDS             10833..11858
                     /locus_tag="PWT25_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL1022009.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="PWT25_001924"
     CDS             12033..13277
                     /locus_tag="PWT25_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL1022010.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="PWT25_001925"
     CDS             13430..14377
                     /locus_tag="PWT25_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1022011.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="PWT25_001926"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="PWT25_001926"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL1022012.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="PWT25_001927"
     CDS             complement(15279..15521)
                     /locus_tag="PWT25_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022013.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="PWT25_001928"
     tRNA            15642..15714
                     /locus_tag="PWT25_001928"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="PWT25_001929"
     CDS             complement(15833..16471)
                     /locus_tag="PWT25_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL1022014.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16594..17226)
                     /locus_tag="PWT25_001930"
     CDS             complement(16594..17226)
                     /locus_tag="PWT25_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL1022015.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17577..17876
                     /locus_tag="PWT25_001931"
     CDS             17577..17876
                     /locus_tag="PWT25_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL1022016.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6157 a         3150 c         3554 g         5119 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
    16681 tacttcaatc atttcaaagg agttgctact taaatcgata ttttttaaat cagtatcaaa
    16741 aaagctagca ttttctaaat cattagtaga aaacattatt ttagtgaggg aacagtctac
    16801 aaaatctgcg cttgataaga cagagtgctc aaaggtactt tcttttatgt ttgaaccaga
    16861 taaatttaca tagtccattt tacaattctc gaataacgtg ttcccaatat aactttccat
    16921 caatttagta cctgttaatt tacaattgac aaatttcacg cgatgcaaca tgctttttgt
    16981 caatatacag cctgaaaaat cacagttttc gaatatacag tcggtaatct ccattctttg
    17041 aaaatcattg gaagcaaaaa atgcatactt gaaaatagat ttgtaaataa ttggatggca
    17101 gattatttgg ttttcgatga cctctttttg tgaaaggata ttatacagtt caaaatcttc
    17161 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
    17221 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
    17281 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
    17341 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
    17401 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
    17461 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
    17521 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
    17581 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
    17641 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
    17701 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
    17761 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
    17821 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
    17881 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag gctaggttca
    17941 tttatttgaa cagagtctgt atttttttat tctaaataaa
//
LOCUS       DANJLF010000051        16715 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000051 DANJLF010000000
VERSION     DANJLF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..563)
                     /locus_tag="PWT25_001932"
     CDS             complement(<1..563)
                     /locus_tag="PWT25_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL1022017.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="PWT25_001933"
     CDS             complement(750..1400)
                     /locus_tag="PWT25_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022018.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="PWT25_001934"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="PWT25_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL1022019.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="PWT25_001935"
     CDS             complement(2912..3910)
                     /locus_tag="PWT25_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL1022020.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="PWT25_001936"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="PWT25_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL1022021.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="PWT25_001937"
     CDS             complement(4926..5657)
                     /locus_tag="PWT25_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL1022022.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="PWT25_001938"
     CDS             complement(5661..6926)
                     /locus_tag="PWT25_001938"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL1022023.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="PWT25_001939"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="PWT25_001939"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL1022024.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="PWT25_001940"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="PWT25_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL1022025.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="PWT25_001941"
     CDS             12268..13770
                     /locus_tag="PWT25_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL1022026.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="PWT25_001942"
     CDS             13772..14509
                     /locus_tag="PWT25_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1022027.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="PWT25_001943"
     CDS             complement(14554..14727)
                     /locus_tag="PWT25_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022028.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="PWT25_001944"
     CDS             14703..15368
                     /locus_tag="PWT25_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022029.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="PWT25_001945"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="PWT25_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL1022030.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         4863 a         3628 c         2823 g         5401 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacatttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaa
//
LOCUS       DANJLF010000052        16575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000052 DANJLF010000000
VERSION     DANJLF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            83..1081
                     /locus_tag="PWT25_001946"
     CDS             83..1081
                     /locus_tag="PWT25_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1022031.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="PWT25_001947"
     CDS             1105..1389
                     /locus_tag="PWT25_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022032.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="PWT25_001948"
     CDS             1470..1868
                     /locus_tag="PWT25_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1022033.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="PWT25_001949"
     CDS             1878..4421
                     /locus_tag="PWT25_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1022034.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="PWT25_001950"
     CDS             4443..6785
                     /locus_tag="PWT25_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022035.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="PWT25_001951"
     CDS             6801..8975
                     /locus_tag="PWT25_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HDL1022036.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="PWT25_001952"
     CDS             8965..9387
                     /locus_tag="PWT25_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL1022037.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10330..11241
                     /locus_tag="PWT25_001953"
     CDS             10330..11241
                     /locus_tag="PWT25_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HDL1022038.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11728..11994
                     /locus_tag="PWT25_001954"
     CDS             11728..11994
                     /locus_tag="PWT25_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022039.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12066..12407
                     /locus_tag="PWT25_001955"
     CDS             12066..12407
                     /locus_tag="PWT25_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022040.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12462..12881
                     /gene="ssb"
                     /locus_tag="PWT25_001956"
     CDS             12462..12881
                     /gene="ssb"
                     /locus_tag="PWT25_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1022041.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12958..13254
                     /locus_tag="PWT25_001957"
     CDS             12958..13254
                     /locus_tag="PWT25_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022042.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13269..13700
                     /locus_tag="PWT25_001958"
     CDS             13269..13700
                     /locus_tag="PWT25_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL1022043.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13882..14187
                     /locus_tag="PWT25_001959"
     CDS             13882..14187
                     /locus_tag="PWT25_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022044.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            14591..15079
                     /locus_tag="PWT25_001960"
     CDS             14591..15079
                     /locus_tag="PWT25_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL1022045.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            15066..16370
                     /locus_tag="PWT25_001961"
                     /pseudo
     CDS             15066..16370
                     /locus_tag="PWT25_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         5931 a         2446 c         3397 g         4801 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagcc ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacagaaact tgcgagagag ttggagtaaa acaagtgtag
    10201 aggaaacttg aagacttgtt cgaacacaaa cttacaagag aggggagtaa agcagttgca
    10261 gaggaatcag gaagatttgt tcgaacagaa acttgcaaga aagcattatt aaattgaagg
    10321 tgatgaaata tgaagtattt attgcatggc tattttattg attattctac tcgcaatagt
    10381 agtactttag cgattctatg tttgcttcat gaattgagag tggttactag aaaacagcta
    10441 tacaaactat tgaattttga cagatcaatt agtaaacaag gagtaacgaa tgctttagtg
    10501 agattatatg aaaaacagtt aatcgatcga aaaaaatatg gaacagaaat gtattattat
    10561 ttgacgaaag agggacatca aagcataggt gggtattata cgttgccgaa agttcctgaa
    10621 tataatttga atcaccattt acaaattaac aattatttga ttaaaggaat ggcattggca
    10681 aaagaccacc cacatttaaa acggattatt tcagaaagac gtaccgtcta tgaaacgaag
    10741 gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc cagattttat ttttcgtttt
    10801 cgagacaaag aaaatcatga gatcaattgg tcctttgaaa ttgagctaac gttgaaaaca
    10861 cgtcgtcgct accgtgaagc gatttttccg aaatatattc gtgagttgcg aacacgtcct
    10921 gaatcacatt taatttatgt gacacctagt ggcgcaattc aagaagaact tcatgcgttt
    10981 aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga gtcaacaaga attgtttgag
    11041 cggttgcata ttttttcagc tttagagttt gaagagggat taaaagcatt attagagaat
    11101 gatccagcag tcaattggcg gacagtagaa cgagaaaaaa tttatccgaa tggcgctgtt
    11161 caaagtcgtt tgaatttatt tgaagaatca actgaaaatg aggaaaaacc aaaagtgaag
    11221 tttacgcctg aagtattgta aaagagggtg ttcgcttcgc tctttttttg tgccagtttg
    11281 ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt ttaggaagga ctaacttgtt
    11341 tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc ctaatcgtat agcagtcatt
    11401 cggcgagtat aacctaaaac ataacctgcc aattgccaag ggtaaaatgc acgctgcgcg
    11461 cccttggcaa tcgtcagaac atgttttcga gccggttatg ccgaacgacc gctatacgaa
    11521 aagggcctct aaacagaagt ccactttgat ccttgacaac tgaaacccaa aatcaaacaa
    11581 gcaggccttc gtctaaaaac gagccgaatg aagaccttgg gggttgcacc cccaaacccc
    11641 cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg cgaaaaaatc aaataaaaca
    11701 ttctattcaa gaaaggaaga aacaaccatg acaactaaaa acgagcaact gaaagaacag
    11761 gcaagaaaag agttgcaaga aagaggtttg attattgagg ggagctttga aggagatttt
    11821 gaaacctata ttggttgtta tgcaagaccg ataaacaaac ctacagcctt agacccaatg
    11881 aacgaacaag aagtaaaaga acaagaaaaa catgcagtga atggttttcc gcaagatttc
    11941 actgaatggt atgagtggga aattaacaac gggaagcttg aaaacttctt ataaaagtca
    12001 gatgacgtgc aaagagaatg attctctttg tacggtaaaa atataaagaa agaaggaaaa
    12061 agaaaatggc aaaaaacaca ccttatcaag taggagacat tttttatagt agttggggct
    12121 acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt aacagaaaaa acagcatggt
    12181 ttcagcctat tcaaaagaaa acggtcgagc attattcgtc catgagtgga aaagttgttc
    12241 caatgaaaga caagtttata gagtatcctt tagcaaaaac taaagacagt attgtaaaaa
    12301 aacgaattga tccagaaacc tatcctgata tgttatgtgg ttatcgtagt tgggacacgt
    12361 ttcatagata taaaaatcaa gctgtttatg aaagtagtta ttattaaggg gatagtttcc
    12421 cctttcaaaa ataataaata gaagaatgga gagaaaaaat catgatgaat tcagtgaatt
    12481 tagtaggacg gttaacaaaa gaagtggatt taaaatacac accaaaaggc aatgcgacag
    12541 gcacatttat tttagctgtt aatcgaaatt atacgaatgc aaacggcgag cgtgaagcag
    12601 attatatccg ctgtgtcatt tggcggaaag ctgcggaaac tttagcaaaa tatactggaa
    12661 aaggaagttt gattggcatt aatggacgtc ttcaaacgag gagctatcag aatcagcaag
    12721 gacagaccat gtatgttacg gaagttctag tgacagattt ctatttgtta gagagtaaag
    12781 aaatcaatga gcaacggtca aaatcaatgg cgaataatca aaataattct tcaattccag
    12841 gaaatgagat tcaaatttca gatagttatt tgccctttta agtgaaaaat aattttgttc
    12901 tatacctgat ttcttcaggt atagaacaaa aaataaatag agatgaggcg attttttatg
    12961 aaaatcattg acagttacga tcaacgaacc atgcaatatt tgggctcgtt tgattataca
    13021 gatgaaaata ttattaatta tgttgcaagt ttacctcctt atcaaagcgt tcgcttggta
    13081 gattttgcaa gtgatgaatt ggtattaaca acgatgggca actttttaga ttatgtcgga
    13141 gacaaaaaat ggttggaaaa aattttgtct aaattgattg cgaaacaaac aggagaagaa
    13201 cccataaatg cgattcaatt gttcaatcga tatgaacaag aaaacaatga ttaaagagga
    13261 gagaaaaaat gaaaagatta aaacgaagta tttttattgt gctagcattg attgattttg
    13321 ttgggacagg ttgtgaagca attgacgttc aaaaagctcc cttatcggaa ataaaacaag
    13381 caataacaaa acaagatgaa gaacaaccgc taaatacaca agtagaaata gtgaatcaaa
    13441 ataaaccgag tttttctgaa gaagattttt cgttagaaaa tggaaccagg caattttttt
    13501 cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc acttttgcat aaaagcagga
    13561 tgccaacaga agaacgagac cgattataca ttaagccaac tggctggaaa caaaagaaaa
    13621 tgaagaatgg cgattatctt tataatcgtt gtcatttgat tggctatcaa cgtgcgacaa
    13681 gtttccccat aagtatgtaa tacgaaacgg gaggattaat cttaagatta atcacaacta
    13741 acctatttga cagttggaat gaaagctatc atgtctgctg aatcaactgg tctgatactc
    13801 ctacatgcaa taaagtctac taggaagtta ttgtatgaag ctaggtaaag gcggcagaac
    13861 aatttctaag tgtggaatca tatgaagttg gatatgccgg gacgctataa attactcagg
    13921 gtgagactgt ttttaattaa actgacgaac ttgcgaatgt acgggtctat aaccgactta
    13981 tattcgaaag atataagatt gcgaatgcaa agaacattat cgaaaagtaa gaatgttggt
    14041 catgaaattc gagatggaag actagcggtg tatctgtcaa gagttggtaa aacatgtttg
    14101 attgattcag atgagagtgg gaatgcgaca aaacgtttta cagaagaaat agagcaacta
    14161 gcagaactga atatacgata tttttaaata ctttagcgga gaataaaaaa atcctctaca
    14221 gaaaactacg gagggcaaca tgtgctattg tttcaacaat acatttttaa tttttaccat
    14281 aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat taaaaatgaa aaaatggaag
    14341 cggttactta ttcttttgaa tctaagataa aaattcaaaa gaataacata acccaaatat
    14401 gattatactt gagattctaa atatttatcg aaaaggaatt tgttttaagg aaaaatgatt
    14461 ctatggacgg ttattgattt atatttacca aatggctaat aaaatagtat ttcttatatt
    14521 gtatagtttt tgtttttatg tgaaaacatg agcaatagaa ttgtactagt aggataagga
    14581 tggatggaaa atgaaaaaaa ctatttttgt tttagcgatt tttatatgtt ttgggctact
    14641 tttggtaggc tacttgttgt ttaattctaa tgaaatgaga actatttcta cgaatgactt
    14701 gttaaaaaaa agtgaattaa aggataaaaa aaaagaatct cttgccttta taatgtttac
    14761 tcaagaagag tgtgagtatt gtatagcaat aaatccaatc atagatagtg taaataagga
    14821 aactttgaat aagataaaga aatatgatat atctgtagat tttcaatcta aacaaaagtg
    14881 taaaattaag tttgttccga ctattgttgc atatagtaag aataaagaag tggggagaat
    14941 tgacaatgaa ttagataatt tgtatcgaaa aaagcaagat ggttcaattg taatagacaa
    15001 gaaaaaaaca aagacattgc ttatagattg gatgaaaaaa atggataaac aaggagaata
    15061 tttgtatgga gaagattaaa cagacatttc cgttaattaa taaatattgg aataagaaaa
    15121 aaatttttat attttttatt acgtttctgc tgtcatcttt attttctcta attttcccgt
    15181 atctatttag taatgttatt gatttatatt tagagaatga gttatcacag aatattgtgc
    15241 gaatagtaac gattcttata tttcagttat ttttagtctt aattaaagaa gtgtatggat
    15301 atataaaaac tatttatgca tttgaagttg atttatcaat gaaaattgat tttcttaagg
    15361 ttttacaaaa aaaaaatctt tttttttcgc aaacgaagca ttcaggagaa attcaattta
    15421 ggatgttttc ggatatttct actatcgtaa atactatctt tattttttct gttgaattac
    15481 ctgtgatagg cgttttagtt cttgtagcgg tatttattat aagtaaagtg aatcttattt
    15541 ttctgatttt ttcactaata atgatgggag ctatggtgtt acagtataat ttatttcaat
    15601 taccgcttaa gaaaacaatc gagaatacaa aaagtgttaa tcaaagtatt acaggagaat
    15661 tgaatgcaca ttttgatcaa ttagaaatta taagcgcata taattttgag aatatctcta
    15721 ttttaaaatt cgattctaga tttaaagaat tatttaatgt tttcaaaaaa caaaataaat
    15781 tactaacact gggaagctgt ttggcacagg ttattttcca attatggatt ggaggaatat
    15841 ttcttattga tgttatttta ttagctaaag gaacaataag tattactaat tttgtaatag
    15901 tatttatgta cgctaacatg gtccctactt tatggactac tttaattagt attatttgga
    15961 aatggcaaga ctattcagtg tcattaaata gatattacga atattattca aataacaaca
    16021 aagtggctgg cacaaaaaaa ataaataaaa taaattctat tactggtcaa tcattaaatt
    16081 tttcatataa caaaagaaag gtattaagca atataagttt ttcaactgaa atgaataaac
    16141 tattcatgat atcaggagaa aatggaacag ggaaaacaac actgatgaaa ttatttacta
    16201 aacagttgcc ggttagtaat aatcaattat tcgtcaacgg gatagatatt aatatgattg
    16261 ataacgaatc attaagaagg ttaatttcaa tcgcaacaca aataccatat ataattaata
    16321 gctccgtgaa ggagaatatt ttaatgggga ctaaaaacta tgataaaaaa aaattcagta
    16381 aattaataga actattggga ataagtaaat ttatgacgaa attaggtatt actttggacg
    16441 acaatattta tgatgaaaaa atattattat ctggaggaga aaaacaaaaa ataagtttag
    16501 ctagggcgct tattaaatca gcttcagtta ttatattaga tgaaccattt agaaatttag
    16561 ataatgaaac tcgcg
//
LOCUS       DANJLF010000053        16373 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000053 DANJLF010000000
VERSION     DANJLF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="PWT25_001962"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="PWT25_001962"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL1022046.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="PWT25_001963"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="PWT25_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL1022047.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="PWT25_001964"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="PWT25_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL1022048.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="PWT25_001965"
     CDS             2857..3732
                     /locus_tag="PWT25_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL1022049.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="PWT25_001966"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="PWT25_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL1022050.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="PWT25_001967"
     CDS             4629..5063
                     /locus_tag="PWT25_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL1022051.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="PWT25_001968"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="PWT25_001968"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL1022052.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="PWT25_001969"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="PWT25_001969"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL1022053.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="PWT25_001970"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="PWT25_001970"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL1022054.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="PWT25_001971"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="PWT25_001971"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL1022055.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="PWT25_001972"
     CDS             13334..14260
                     /locus_tag="PWT25_001972"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL1022056.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="PWT25_001973"
     CDS             14442..15335
                     /locus_tag="PWT25_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL1022057.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="PWT25_001974"
     CDS             15469..15594
                     /locus_tag="PWT25_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1022058.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="PWT25_001975"
     CDS             complement(15728..16360)
                     /locus_tag="PWT25_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022059.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5815 a         2791 c         3492 g         4275 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aat
//
LOCUS       DANJLF010000054        16309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000054 DANJLF010000000
VERSION     DANJLF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..257)
                     /locus_tag="PWT25_001976"
     CDS             complement(<1..257)
                     /locus_tag="PWT25_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022060.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="PWT25_001977"
     CDS             complement(273..923)
                     /locus_tag="PWT25_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022061.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="PWT25_001978"
     CDS             complement(934..2106)
                     /locus_tag="PWT25_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL1022062.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="PWT25_001979"
     CDS             complement(2125..2997)
                     /locus_tag="PWT25_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1022063.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="PWT25_001980"
     CDS             complement(3019..3939)
                     /locus_tag="PWT25_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022064.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="PWT25_001981"
     CDS             complement(3939..4208)
                     /locus_tag="PWT25_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022065.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="PWT25_001982"
     CDS             complement(4205..4585)
                     /locus_tag="PWT25_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL1022066.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5052..6515)
                     /locus_tag="PWT25_001983"
     CDS             complement(5052..6515)
                     /locus_tag="PWT25_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL1022067.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6579..9596)
                     /locus_tag="PWT25_001984"
     CDS             complement(6579..9596)
                     /locus_tag="PWT25_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1022068.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9747..10586)
                     /locus_tag="PWT25_001985"
     CDS             complement(9747..10586)
                     /locus_tag="PWT25_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1022069.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10731..10976
                     /locus_tag="PWT25_001986"
     CDS             10731..10976
                     /locus_tag="PWT25_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022070.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11039..11461
                     /locus_tag="PWT25_001987"
     CDS             11039..11461
                     /locus_tag="PWT25_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022071.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11555..11872)
                     /locus_tag="PWT25_001988"
     CDS             complement(11555..11872)
                     /locus_tag="PWT25_001988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022072.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11899..12105)
                     /locus_tag="PWT25_001989"
     CDS             complement(11899..12105)
                     /locus_tag="PWT25_001989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022073.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12086..12268)
                     /locus_tag="PWT25_001990"
     CDS             complement(12086..12268)
                     /locus_tag="PWT25_001990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022074.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12282..12632)
                     /locus_tag="PWT25_001991"
     CDS             complement(12282..12632)
                     /locus_tag="PWT25_001991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022075.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12658..12960)
                     /locus_tag="PWT25_001992"
     CDS             complement(12658..12960)
                     /locus_tag="PWT25_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022076.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12963..13196)
                     /locus_tag="PWT25_001993"
     CDS             complement(12963..13196)
                     /locus_tag="PWT25_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022077.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14182..14504)
                     /locus_tag="PWT25_001994"
                     /pseudo
     CDS             complement(14182..14504)
                     /locus_tag="PWT25_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14627..15742)
                     /locus_tag="PWT25_001995"
     CDS             complement(14627..15742)
                     /locus_tag="PWT25_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL1022078.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15748..16146)
                     /locus_tag="PWT25_001996"
     CDS             complement(15748..16146)
                     /locus_tag="PWT25_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL1022079.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4312 a         3415 c         2555 g         6027 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct aatactatat
     4921 ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa aatgagagat
     4981 acaaatatat caaatattta aaaagtaacc acattttcta taccactcct tatcagaaac
     5041 agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt tgttaaacta
     5101 ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca
     5161 taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg
     5221 tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt tcctgacaaa
     5281 gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg ttctccttga
     5341 ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa ggaactagaa
     5401 attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg accagctact
     5461 tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt tttatcgtag
     5521 ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata ggtttttgcc
     5581 ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc ttttaacagt
     5641 ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc
     5701 gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta
     5761 tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg actttgtccg
     5821 cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc attttcttgt
     5881 ccaattttac gagcagattc accgatttta cggataaacg tttctacaga cgcgtctttt
     5941 tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc ttgtatcggt
     6001 gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg tgcctgcggt
     6061 acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg ttccgttttt
     6121 gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga gctttcatca
     6181 gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt gtcttctggt
     6241 ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg agcgttcatt
     6301 tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg attttcttct
     6361 ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt tgtcagttga
     6421 ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg acctactaat
     6481 aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt cttcaatcaa
     6541 tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg tttcatttta
     6601 cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc aattaacaca
     6661 tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt
     6721 ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt ttgtttttca
     6781 ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt atagacaata
     6841 tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc aaaggatact
     6901 ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc aacgactgta
     6961 tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt ttcccctacc
     7021 gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt gtctggatca
     7081 aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc tttcgcagtc
     7141 actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc ctcttctttg
     7201 tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg
     7261 tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt ttcttttacc
     7321 gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt cacccaatat
     7381 ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac taattcttgg
     7441 tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt agaaatttct
     7501 ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg ttgtacttct
     7561 ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc ataatgctca
     7621 gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt ttctggttct
     7681 tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc ttcaatcagg
     7741 aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa gtctacttca
     7801 cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg cgtcgtgtta
     7861 tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta gttgtctggc
     7921 acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt tttgtttgcc
     7981 ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt tttcacaagg
     8041 actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa ttcgatattt
     8101 tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc cgcattatag
     8161 acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt tgcagttaac
     8221 gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag aatataccca
     8281 gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc gtctttttca
     8341 tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt atattgtttt
     8401 gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt tttccctgtg
     8461 acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat agttagtcca
     8521 gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg atctacatag
     8581 gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga aattcgtccg
     8641 tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct tacttgaacg
     8701 ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt tgtatcctta
     8761 acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga aggatcttca
     8821 agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt agattgtcca
     8881 atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt ttgtgctaat
     8941 acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa agaaatcttt
     9001 tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata ttgaataccc
     9061 gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga
     9121 accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat attcacatct
     9181 ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt cttttccaag
     9241 attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct
     9301 ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac ctcttgagtc
     9361 attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt
     9421 tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc agtcttcatt
     9481 acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac agatcctaac
     9541 atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt aaacatattt
     9601 ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg gacttcgaca
     9661 ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat tctagtcttc
     9721 atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt ttgtgacccg
     9781 tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga atttggatcg
     9841 ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac ctttccacta
     9901 ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc accatttccg
     9961 ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc tccacctgtg
    10021 cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg ttgtgtaggt
    10081 gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc accagttgtt
    10141 cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt
    10201 gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc ttgaatcact
    10261 gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc attaaatact
    10321 aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc cgctaatttt
    10381 tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca gacaattgtg
    10441 tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt ccaaactttg
    10501 ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac tacgattcct
    10561 agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata aatttattta
    10621 caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag atttttttat
    10681 tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac atgcaactta
    10741 ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt aaacaagctg
    10801 ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga aataatatca
    10861 aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta tatctaagag
    10921 aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt caatgaggcg
    10981 ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg gaggttctat
    11041 gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg ctattattgc
    11101 tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat cgactactac
    11161 aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg
    11221 acaagattta attgatcttg gttcttctca aattactact gtcttatttg aaaagaaaaa
    11281 tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca aagtaacaaa
    11341 taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca agttaaagat
    11401 tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact cactgaaata
    11461 agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat cattttataa
    11521 aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc tcaatttctt
    11581 ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc tccccttctt
    11641 ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga taccaaccaa
    11701 caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat tcatttttcc
    11761 atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca caaatttcgt
    11821 catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc atcttaactt
    11881 ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt gtttactagt
    11941 taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag cgtcgttttt
    12001 ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt cctgcatttc
    12061 ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc attccctcat
    12121 tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc acttgtccta
    12181 taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg aaactaatgg
    12241 ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt tgttatttct
    12301 tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata
    12361 aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca ataaaattct
    12421 ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat ttttgaatca
    12481 aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg gagtgcctgt
    12541 tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc ttttatttca
    12601 ttgttctcaa atactttata actttttttc attgttagtt cctctttcat tcctctctca
    12661 attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa tccttaatgc
    12721 tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc ttttagcagt
    12781 tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat tcgaaccgat
    12841 tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag tgattgctcg
    12901 attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt tagacaacat
    12961 ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa tccacaaata
    13021 aatgaattca tattccaagt accattccct tcaactacaa cttttctcct gtcctctttc
    13081 ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa tttgtttgta
    13141 taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat tgtcatttcc
    13201 tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca acgaatatac
    13261 ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc ttttacttgt
    13321 tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt ttttcatcca
    13381 ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt ttcttgttca
    13441 tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt catcaatcat
    13501 caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc ccaaactcta
    13561 aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca tcatatgttt
    13621 ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac ccatccatac
    13681 gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag tccctgttag
    13741 acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga
    13801 aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc
    13861 tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat catcaaatgt
    13921 tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt cgatagcgat
    13981 tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa aactaacaaa
    14041 caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata gttgttagtt
    14101 taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca attggggcaa
    14161 acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg
    14221 ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt ttgttttcct
    14281 ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg aattaatagg
    14341 tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa
    14401 caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg agaaaaaaac
    14461 agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt
    14521 gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg ccgattaaac
    14581 aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat gtgtccaatt
    14641 cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa atctgcgttt
    14701 ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct gcttgatctg
    14761 atagttttgt tgccattgga tctagattca attctcttac acaacattcc caaaaattca
    14821 atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca tgacgagcaa
    14881 ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca aatgtttccc
    14941 ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac tttcgttgcc
    15001 gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc
    15061 attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac gtactttcgc
    15121 tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga ctagtcgtat
    15181 cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta aataattgtg
    15241 cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag
    15301 attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca ggacctaatg
    15361 aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct ttgtttcttt
    15421 ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat tctttcaatt
    15481 cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct aattcttgtt
    15541 ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga atggctaaat
    15601 ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat gagcgatatt
    15661 tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat ttacgagttg
    15721 cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata ttaagaaaga
    15781 attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt ttagataaat
    15841 tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga tttcttcaat
    15901 ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag aaatatagac
    15961 atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact cattcagtaa
    16021 gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt ttataaagaa
    16081 tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa atttgttttt
    16141 taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa tcgaattaca
    16201 cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa aactaaacac
    16261 ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaa
//
LOCUS       DANJLF010000055        16225 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000055 DANJLF010000000
VERSION     DANJLF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            138..1475
                     /locus_tag="PWT25_001997"
     CDS             138..1475
                     /locus_tag="PWT25_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL1022080.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="PWT25_001998"
     CDS             1523..3652
                     /locus_tag="PWT25_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL1022081.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="PWT25_001999"
     CDS             3627..4316
                     /locus_tag="PWT25_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022082.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="PWT25_002000"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="PWT25_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="PWT25_002001"
     CDS             complement(5546..5740)
                     /locus_tag="PWT25_002001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022083.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="PWT25_002002"
     CDS             complement(5790..6230)
                     /locus_tag="PWT25_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL1022084.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="PWT25_002003"
     CDS             complement(6234..7079)
                     /locus_tag="PWT25_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL1022085.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="PWT25_002004"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="PWT25_002004"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL1022086.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="PWT25_002005"
     CDS             complement(8703..9650)
                     /locus_tag="PWT25_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1022087.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="PWT25_002006"
     CDS             complement(9873..10223)
                     /locus_tag="PWT25_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL1022088.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="PWT25_002007"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="PWT25_002007"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL1022089.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="PWT25_002008"
     CDS             11646..11966
                     /locus_tag="PWT25_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL1022090.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="PWT25_002009"
     CDS             11988..12452
                     /locus_tag="PWT25_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1022091.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="PWT25_002010"
     CDS             12657..13262
                     /locus_tag="PWT25_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL1022092.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="PWT25_002011"
     CDS             complement(13301..14590)
                     /locus_tag="PWT25_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL1022093.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="PWT25_002012"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="PWT25_002012"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL1022094.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16225
                     /locus_tag="PWT25_002013"
                     /pseudo
     CDS             15700..16225
                     /locus_tag="PWT25_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5134 a         2971 c         3297 g         4823 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttga
//
LOCUS       DANJLF010000056        15952 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000056 DANJLF010000000
VERSION     DANJLF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(388..810)
                     /locus_tag="PWT25_002014"
     CDS             complement(388..810)
                     /locus_tag="PWT25_002014"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL1022095.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(824..2230)
                     /gene="atpD"
                     /locus_tag="PWT25_002015"
     CDS             complement(824..2230)
                     /gene="atpD"
                     /locus_tag="PWT25_002015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL1022096.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2253..3155)
                     /locus_tag="PWT25_002016"
     CDS             complement(2253..3155)
                     /locus_tag="PWT25_002016"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL1022097.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3169..4725)
                     /locus_tag="PWT25_002017"
     CDS             complement(3169..4725)
                     /locus_tag="PWT25_002017"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL1022098.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4748..5290)
                     /locus_tag="PWT25_002018"
     CDS             complement(4748..5290)
                     /locus_tag="PWT25_002018"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL1022099.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5277..5801)
                     /gene="atpF"
                     /locus_tag="PWT25_002019"
     CDS             complement(5277..5801)
                     /gene="atpF"
                     /locus_tag="PWT25_002019"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL1022100.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5892..6107)
                     /gene="atpE"
                     /locus_tag="PWT25_002020"
     CDS             complement(5892..6107)
                     /gene="atpE"
                     /locus_tag="PWT25_002020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL1022101.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6160..6879)
                     /gene="atpB"
                     /locus_tag="PWT25_002021"
     CDS             complement(6160..6879)
                     /gene="atpB"
                     /locus_tag="PWT25_002021"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL1022102.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7181..10024)
                     /gene="rnr"
                     /locus_tag="PWT25_002022"
                     /pseudo
     CDS             complement(7181..10024)
                     /gene="rnr"
                     /locus_tag="PWT25_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10027..10782)
                     /locus_tag="PWT25_002023"
     CDS             complement(10027..10782)
                     /locus_tag="PWT25_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1022103.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10885..11121)
                     /gene="secG"
                     /locus_tag="PWT25_002024"
     CDS             complement(10885..11121)
                     /gene="secG"
                     /locus_tag="PWT25_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL1022104.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11239..11517)
                     /locus_tag="PWT25_002025"
     CDS             complement(11239..11517)
                     /locus_tag="PWT25_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL1022105.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11630..12598)
                     /locus_tag="PWT25_002026"
     CDS             complement(11630..12598)
                     /locus_tag="PWT25_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1022106.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12813..12857
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12881..13855
                     /locus_tag="PWT25_002027"
     CDS             12881..13855
                     /locus_tag="PWT25_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL1022107.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13860..14435
                     /locus_tag="PWT25_002028"
     CDS             13860..14435
                     /locus_tag="PWT25_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1022108.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14502..15641)
                     /locus_tag="PWT25_002029"
     CDS             complement(14502..15641)
                     /locus_tag="PWT25_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL1022109.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5296 t
ORIGIN      
        1 tctgagccag aatgtggttc attctgactc agatttgatt ttgcacaact catcagtcct
       61 tattgacaaa gagcccaaaa gtcttgtcgc agtcccttat tccaaataaa cggtgttcaa
      121 tcatctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttccattt
      181 gtttctctaa tactcaggac aaaacgctga tttcacaacc tccgatatac ggtgttccag
      241 aagtagcttc ttcgaaaaat aagctgagat ttcacaaaaa tttgagaagc aattttcgta
      301 aattccttct tatgtcttga agctgaccac ttctgtcata acctcttttt actgttctta
      361 aacacggata aacatccgca tctcatttta tgagtgctta gatactttga tacgattgat
      421 tgctcggtgc aacgcaacag ttgcccgttt caattcgttc gtatcttgtt ttgcttttgc
      481 ttcttcgatt tggcgttctg cgcgttgttt tgcacgttcc gcacgagata catcgatatc
      541 acgttcacgt tccgcactat cagcaacgat tgaaacaaca ttatcacgaa cttccatgat
      601 tccgccatta acagcgatcc aatcgacgtg ggtatctgaa tctgttcgtt ttacacgaac
      661 ttcatcaatc gtcaatggaa cgatgattgg tgcatgttta ggcaaaatcc caagctcacc
      721 agctgtcgta cgtgcaacga cgatagctgc atggtgatca tagaccaaac cggcaggagt
      781 taccacattt actgttaaat attgatccat ggggcacccc tttctagtag cctagtgttt
      841 tcgctttttc tacgacgtct tcgattcggc caacacttcg gaaagcttct tcaggaagat
      901 catcgtattt gccttccaag atttcgcgga atcctttcac tgtttcttct acagggacat
      961 atgaacctgg aagaccagta aattgttcag ccacgttaaa gttttgtgac aagaagaatt
     1021 ggactctgcg ggcacgagaa actaacactt tttcttgatc ggataattca tccataccta
     1081 agatagcaat gatatcttgt aattcacgat atcgttgcaa tacgtgttgg acttctgttg
     1141 ctactttata atgttcttct cccacgattt caggtgctaa agcacttgaa gaagaagcca
     1201 atggatctac cgctggataa atcccttgtt ctgttaattt acgttccaag ttggttgtcg
     1261 catccaaatg ggcgaatgct gttgccggcg ccgggtcagt gtagtcatcg gctggcacat
     1321 agattgcttg gatcgatgtg atcgaacctt ttttcgtaga tgtgatacgt tcttgcaatt
     1381 gacccatttc tgttgccaat gttggttgat aaccaaccgc agaaggcatc cgtcctaaca
     1441 aggctgatac ttcagatcca gcttgagtga aacggaaaat gttgtcgata aacaacagta
     1501 catcttgacc ttctacatca cggaaatatt ccgcaatcgt caaaccagtc aaagcaacac
     1561 gcatacgtgc acctggtggt tcgttcattt gtccgaacac catggctgtt ttttcgataa
     1621 ctcctgaatc tttcatttca taatacaagt cattaccttc acgagtacgt tcacctacac
     1681 cggtaaatac agaaatacca ccatgttctt gggcgatatt atggatcagt tcttggatca
     1741 gtacggtttt accaacaccg gcaccaccga atagtccgac tttcccacct tttaaatatg
     1801 gggcaagcaa atcgataact ttgatccctg tttccaaaat ttctgtactt gtacttaatt
     1861 cgtcaaaggc tggtgctttt ttatgaattt cgcttctttc cgcatcttct gggaaaggtg
     1921 tttctaaatc gatcgtatct cctagtacgt tgaatacgcg acctaatgtt tctttaccta
     1981 caggaacaga gattgctttg cctgtatcaa tgacttccat tcctctttgt aaaccatctg
     2041 ttgattccat cgcaatggtc cggatcactc cgtcccctaa ttctaacgct gcttcaagaa
     2101 caacttttga tttattttcg tcatttttat aaacaactaa tgcgttgttg atatctggta
     2161 aggattggtc taaagaaaat tccacgtcga caacgggacc gattacttga acaatcttgc
     2221 ctgaactcat cttttttcct ccaatctcaa tactattcta gtgccgaagc tccggcaaca
     2281 atttcggtaa tttcttgggt aatagcccct tgtcttgcac ggttgtaaga aaccgtcagc
     2341 tcatcaatga tattagcagc attatcagtt gctgttttca ttgctgtcat accagcagca
     2401 tgttctgctg ttttggcgtc aacgatcgca ccgtagatca aactttcagc atactgaggc
     2461 aataactgag acaagatttc ttcttttgaa ggttcaaaaa tatattcttg ttcgaaagta
     2521 gttgcttctt ctggatctaa gtctgagatt ggcagcattt tttctacacg aaactgacta
     2581 gttagcgaat tgatatgatg gttataacat acgtataatt catcgaatac ttcgttttgg
     2641 tacatagttg ttgccatatt tacgattttg cggacttcat caaagcttgg ctgatcagat
     2701 agattgcgca gctcatatgc tagattgata ccgcgtgctt taaagaagtc tgcaccagtt
     2761 ccgccaattg cgatcatcac gtaatcatcg ggtgattggt ggtcttcttc taaaatagac
     2821 atcgtttgtt tcaatatcga gctgttatat ccaccgacta aaccgccatc tgcagtgatg
     2881 acgatataac ccgttttctt aacaggacgg ctgagcaaca tgctgttgta attgatatcc
     2941 cctctaggat tttctgaggc aatatcattt aactgagtag cagtcaaatg cgtaacgatt
     3001 tctcttactt ttgttgcata aacctgaaac ttctgtgaag aagcttcaga ttttgtcaac
     3061 ttagaagcag acaccatctg cattgcccgc gtgatttggc tcgttttttt cgttgaagcg
     3121 atacgtgtct tgatttcatt taatgaagca cccattcatc tcacctcact atgcattttg
     3181 aatggatttc aatttatctt ctgtagagga attatttcct tttgtagcta agaacatgtc
     3241 tttatattcc tgaatcgcac tgttcaacgc ttcttcttct ggaagatctt ttgttgtacg
     3301 gattgtttca aagatatctg aatgattggt atccagatat tcgaataatt catgttcgaa
     3361 gtctaaaata ctgtctacag ggatactatc taagaatcca tgtgtcaaag catacaaaat
     3421 cactacttgt ttttcaacag ctagcggtgc atgcaatttt tgtttcaaga tttctactgt
     3481 acggcgtcca cgatttagtt ttgcttgtgt tgctgcatcc aagtcagaac cgaattgtgt
     3541 aaatgcttct aattcacggt aacttgctaa gtctagacgt aaagtcccag caactttttt
     3601 cattgctttg atttgcgcag aaccccctac acgagataca gatagtccgg catcaaccgc
     3661 tggacgagta cctgcgtaga acaagtcgct ttctaagaag atttgtccat cagtgatcga
     3721 aataacgttt gttgggatat aagcagagat atctccagct tgtgtttcaa caaatggtaa
     3781 ggcagtcatt gaaccgccac ctaaatcatc acttaatttt gctgcacgtt ccaataagcg
     3841 tgagtgtaag tagaaaacat cccctggata agcttcacga cctggtggac ggcgaagcag
     3901 caaggaaagt tcacgataag caacagcttg ttttgaaaga tcatcaaaaa tgatcaagac
     3961 atgtttcccg ttgtacatga actcttcacc cattgcagta ccagcatatg gtgcgatata
     4021 aagcaacggc gctggttgag acgcaccggc attcacaacg attgtgtaat ccattgcgcc
     4081 atattttttc aatgtttcaa cttgtgtacg aactgtagaa tctttttgtc cgattgctac
     4141 atagatacag atcatatctt gacctttttg gttgatgatc gtatcgatcg caatggaagt
     4201 tttccctgtt ttacggtcac cgataactaa ttcacgttgg ccgcgtccga ttggtacaag
     4261 agcgtcgatt gcttttagac ctgtttgcat tggttcgtta acagatttac gttgcataac
     4321 gcctggtgcc attgcttcta ctggacgagc tttgtcagta acgatttcac ctagtccgtc
     4381 aattggctga cctagcggat taactacccg tccaatcaag gcctctccaa ctggaacttc
     4441 catgatcttt cctgttcgtt ttactttatc tccttcacga atggactcga aatcgccaag
     4501 gataatgata cctacatcat ttgattctaa gttttgcgcc attccgtatg agccatttga
     4561 aaattcaagc aactcaccac tcatcgcgtt ttctaaacca tgtgcacgag cgatcccgtc
     4621 acctacataa gttacggtac cgatttcttc gactgaaagc acgttttgat aattttcaat
     4681 ttgttcttta atcaaggcac taatttcttc tgctttgatg gccattcgat tcacctacct
     4741 ctttgtacta tctatttaat tgattacgca tattttctaa ttgcgtgcga atgcttccgt
     4801 cgattactcg atggttagct tcaacaatcg cgccaccaat aatagaagaa tcaacgattt
     4861 gttttaattc gactgattga tagttcattg tttttgcaac attcttttct aattgtattc
     4921 gttgttcgtc tgaaagtgga actgctgttg tgacacttcc caacagcaaa ccttgatgtt
     4981 catcatatcg acgttcatat tcttcgatca tgaacaaaag atcatccatt cggttatacg
     5041 cataaacaac ttctaaaaag tttttaacga ttccatcata gccactaaca agcttgtcca
     5101 tgatttctcg tttttcatga ggttctagac gaacatcact taacatatta cctaatccag
     5161 gtacttgttc atagatttga cgtaagctta cgagatcttg ataaatttct tctgctttat
     5221 ttgaatcgat cgccagttca aataaagctt tcccgtaacg tcttcctact gtatatttat
     5281 ctagtttcat ttgaagaacc tagaccttcg atatattgat tgattaatga ttcgtgcatt
     5341 tctggagata attctttgtt caagattttt tccgcgattt gaagagatag ttctgcaaca
     5401 tcgtctttta cagagttcag cgctgaatca cgttctacag tgatatcagc ttgggcttta
     5461 ctttttagac gagctgcttc ttcttgagca tccttcaaaa tattttggcg gcttaattct
     5521 ccactttctt tcgcattttt gatgatatca gctgcatcag aacgagaggc taacaattgt
     5581 tgttcgcgtt cttgttccat tttcgctgag ttgatgcgag attgttctgc agaatccaaa
     5641 tcattggcga tcttgtcttc acgttttttc aaaatatcgc tgattggtcc ccaagcaaaa
     5701 tgcttcaaga gagccagtag tatcaaaaat gagccgctga caacgatgac atttcctaac
     5761 atcgcattgc caacttctgc aattgccaat tgattcagca taggcgagcc ttcctttctt
     5821 tgaagtactt ctagttttca tgtaaaaaag cagtaacaaa ccaaattgtt tctgccttaa
     5881 tactgattca attaaacacc aaaaactaga atcaaggcga caactacccc taagatagga
     5941 acagcttcga ctagagctac cccgataaac attgttgtac ggaattgacc agacatttca
     6001 ggttggcgag ccattgattc gattgtttta gagataactt gaccgttacc ataaccggct
     6061 ccgattgctg ctcccatgat tgcgattgct gctgcgatat aattcataat tgttttcctc
     6121 ctaaattaga taaatagtag tttgttttta aaacgaatat tattcttcca cttcaatctt
     6181 atggctcata taaaccatag ataaagtaac gaagacaaat gcttggatgc ttccgataaa
     6241 caaggagaat gcgatccaga ccatctctaa tggaattgct aacggcaatg agaaccagcc
     6301 taagcttatt accatttttg caatcaaggt tagcaaaact tcccccgcat aaatattccc
     6361 ataaagacgt aaagcaagtg ttaataagtt tgtaaattct tcaatcagtt tcatcggaaa
     6421 cataaaacta tatggtttca agaacgaatt tacgaaatat cctttgaagc ctaacttttt
     6481 caccccaaat aagtgagtaa gtaagatcat tataaatgcc aaagttaaag taacaaacgg
     6541 gtctgctgtc gggcttttcc aaagtgttgt ttcgttaggt aaaacgattt ttgttactaa
     6601 accgatgttg tttgcaacaa aaacaaataa aaacaatgta aatgccaaca aatggaaatt
     6661 gctcaattct tttctaggca gattatctgt tacgattcct cgactaaaat caatgaccca
     6721 ttctatcgca ttttgtttgc ctgttggttt caacttaaga tttcttgtaa aaaagtaagc
     6781 taagaagaaa acaatcaaac aggtcagtaa caccattata caaactgtac catcaaacca
     6841 aattggtcca atgtggaacg ttagcgagcg ttcatccaat ccatttcacc tcttttcccc
     6901 gaaattcaca taaaatcaat tcatgtgcgt atctggtttt ttccaatcgg tttctaacca
     6961 cacggtatca taccaccatg aaacagaaaa ttcaaaacat tttcatgaaa attagacact
     7021 tttttatttc agtcaaaaac gaagcgaatt tttctttata cacggtgctc ctgatctaag
     7081 aaaaaaccta tgataatcat gttttatcag attaaatgct aacaaatttt atcattaagt
     7141 aaaagcaaaa gcaccgaata tgcgaaaaag gataatgaaa ttatctagag aaatttttta
     7201 atgttcgatc aatttgtcga tcgatatctt ttcgtttcaa atcttcccgt ttatcataag
     7261 attttttccc tttcgccaga ccaatcaata ccttggcata gccgttacga atatagactt
     7321 ttaaaggaac aacagtgatt ccagtatttt tcaattcatt ttcaagccgg atgatttgtt
     7381 ttttgtgtaa taataacttt ctcgtgcgca acggatcatg attaaaaata tttccttgtt
     7441 cataaggact gatatgaaca ttatgcaaga aagcttctcc gttgcggacg cgaataaatc
     7501 catcttttag attgatccgg ctgtttcgta tcgacttgat ctcggttcct tgcaagacca
     7561 tccctgcttc catcgtgtcg atgatcgaat aatcatggcg agctttcttg ttttgtgcaa
     7621 ttaattttcc ctcgcctttt ggcatagctg tttcctccaa ctcattttat tttttctttt
     7681 tcttcttttt aacacctttg taaaatggct tcttcccttt tttcttaccg aacgtttgct
     7741 tactatcgct atatcgtttg ttcttcttgc catttctttc tgggcgtttt cctgcttgtt
     7801 ttccttcgcg ttttcctgat tttccatttc gtgtttttgg ccgttccaac gctgtaacct
     7861 cttctgcgga aacaagttca aaatctactg ctcgttcatc tggatcagct ttgactactt
     7921 ttactcgcac tttctgtccg attttaaaag tcagaccagt tctttcacct acaagagcta
     7981 aatgattttc aacaaaatgg aagtagtcct gtttcaattc attgatatgg atcaatccct
     8041 ctactgtatt cggcagttcg ataaacaggc cgaatttcac aacagagctg atgatcccat
     8101 cgaattcttc ccccactttg tccatcatga attctgcttt cttcatggaa tctacttcac
     8161 gttctgtttc tactgcacga cgttccatct tagaactatg atctgcaatt tcaggaagtt
     8221 ccgtagccca cgaactttga ttgctttcac ttttatcttc actgtaagaa cggatcagac
     8281 gatggacgat tagatcggga tagcgtcgaa taggagatgt aaagtgtgtg tagtaatctg
     8341 ccgctaagcc ataatgccca aaattgtctt ctgaatagcg tgcttgttgc atgcttctta
     8401 gcaacatagt attgataact gcagcttctg gtttgtcttc tactccttcg atcacatgct
     8461 gtaaatcttt tggtgtgatc gttcctttcg ttcctcggac cagaatgccc aaagctgaag
     8521 caaaatcaaa gaagcgctgc attttttctt cttttggctg ttcatggatt cggtaaataa
     8581 acggcagatt taatttatta taatgttcag ctaccgtttc attggccgca agcatgaaag
     8641 attcgatcaa gcgttcacca attcctctgc ttcttagctg gatatcattt gggtgtcctt
     8701 cagcatcaac aaggattttt gcttcgcgat cctcaaaaga aatcgcgcca cgtttgattc
     8761 gcatcgtttc aaggattcga tgcaattcac ccatttcttg gaacataggt acgagctgag
     8821 catagcgttg catcgtttct tcgtcttgat cttctaagat ttgattgaca gctgtgtagg
     8881 tcatccgttc tgttgtttgg atcacacttg ggaaaatatc atgagagact acttgtccat
     8941 ttggatcaat ctccatttcg caactcatgg tcagccttgg aacatgggga ttcagtgaac
     9001 agataccatt cgataaacgt tgcgggatca tcggaatcac tcgatcagtc aaatatacac
     9061 ttgtccctct ttcatatgct tctctgtcca attgactgcc ttgtgtcaca tagtaagaaa
     9121 catctgcaat gtgtactcca agaaaataat taccgttatc taatttttta accgtaactg
     9181 catcatctag atcttttgca tcttctccat caatcgtaac aatgagctga tttcttaaat
     9241 ctctacgtcc agttatatcc ttttcgctga tttgatctgg aacttcatct gcttcagaca
     9301 atacatcatc aggaaatttc gtagggatac cattagccac gacaattgag aggatatcca
     9361 tgccagggtc attcttatga ccaatcactt gcttgattat cccttctaaa ctagtcgcat
     9421 accctttttc agggtaatgg gtcagttcga tcagcacaat acttccgtca actggacgaa
     9481 ctccctctgc tgctgcatat actttaaatt ggttgagctt tctatctttc gggataacaa
     9541 cgccgtaaag atccgtttct tgtatttctt cttctgaata agcaacgaat tcccctgcta
     9601 actgagtagt cgcccggtct cgaatctcga ctactttccc ctctgctccg cgatccatat
     9661 ctgtatttgc tcgcttcatg atatcgattg ctacggtgtc tccatccatt gcaaaaccag
     9721 tcgcttcttt tgggatatag atgtcatctt cttcgggatc aatcgttaca aatccaaatc
     9781 ctcgttcatt tgctcgaaat gttccttcga tcaagattgg ttttaaagga agtttcactt
     9841 ttcctttttt attgaaaact atactctgtt ctctttccat ttgagcaatc gtttgtacta
     9901 gtaatttaaa atcatcgctt ttttggaacc ctaaaccttc agcgatttct tccatagaaa
     9961 aactgcgttt gcgactgctt tccatgaaaa agagaatttt ctcttttatc gtttcttttg
    10021 tcatatttat tcctcattcc aaggtaattt ttctaaaaat tccaagacgt ctttttctaa
    10081 ttcgcgtctt gctgtaccaa ctgtaatcac atgaccactt tcaggatacc atcgaagtgt
    10141 cactcgctga cggctcaact tgcgtgcagt ttcaaatacg ccattagggt cgatcatttc
    10201 atctttccca gcttgagcta aaaatatcgg ctgttggata tctgacaacc tactttctac
    10261 gattgctgcc tgatcttgta tatccattaa ttgttgttca gctaacgagc ggtaagcaac
    10321 tgctttttca ttgatttctt tctctgattt acctgctgtc ttcagcactt gacgtacgta
    10381 tttttcaaag ttttcaggca cgtttgtttt tacaggagac aaaggcgaac agaaaaaacc
    10441 gccgccaatc actgattctt cttctagtgc tcgtacagca aagatgccgc ccattgataa
    10501 tcctaatacg gcaatatcag aaaactgctc agactgtaaa aaatggatgg cttttttagt
    10561 gtctgcccac cagctttctg ctttttcttg caaaatatca tatggctcca ttgttccgtg
    10621 tcctttaaaa agcggcgcat aaactgtata atctgctttt tccaaaaatc gtgcgagcat
    10681 tcgaacatcg tttggacttc ctgagtaagc gtgaagcagc aaaaccgctc gctttccatg
    10741 ttttgcataa aggggttttg gtaattgtct cactcgtctc acctctttcg cctcatttta
    10801 ccatagtttc tttcataact tcttcttaag cgttctcttt gcaataaaaa aagccgcccg
    10861 ccgttttgcg agaggcttat tcaactactt cgaagataaa tatgctagga caaataataa
    10921 gatcatccag atagctccca taactgcagt tgatcgctgc atgacagctt caaagccgcg
    10981 agctttttgt ttgccgaata gttgatccgc accgccggta aatgcactcg ctgcactatt
    11041 ttgcttgctt ggctgcatca taatcgcaat gaccataaca atcgataaaa cgatcacgat
    11101 tcccaaaatt aaattataca tatcgcagcc tccttcccaa aagatatcgt ctctttaact
    11161 ttatcataga taaaagtccg tgacaactct atataaatgg aataacagct gaaagttcaa
    11221 aactttcagc tgttattctt atgactgacg taagacgtct tgatattctg gtttcttttc
    11281 aaactctttt tttgcataag ggcataaagg aatgatcttt ttcccgtctc gtcttgctaa
    11341 ttctactcca gtatacacta gcttttcagc taacttttga ccacgatatg ctggatctac
    11401 aaatgtatgg tcaatgatca tgatgtctgg gcccgcatct gaccatgtca tttctccgat
    11461 ctcttgttgt tcatcattga ataaagcaag acgatttttt tcttctttga tttccatatg
    11521 ccgcactcct caaataactg atattttttt attcctacct tattttagca gtctcctgat
    11581 ctataaacaa ttatttatcc tcgaattact tacaaaaaag aagagaaaat taattcatcg
    11641 ttattctttg ttcaataaat gaaaaaatca ctgtccaata ggttttcgga tctttcatcg
    11701 atgcttccaa gtgttctgct tctggaacaa gcagcagtgc tttatcccct gccgtagcat
    11761 tttgaatcgt gtacgctgct tctgtaggga caaaatgatc tctttcacca tgaatgacca
    11821 ataaaggcag atggttgctg ccaagctgtc tcgtagctga tgcttgcttt aaggagtaac
    11881 cggcgtattt ctgggcataa tgattcgcat atctcatgac cattttttta ggaaagacag
    11941 tgattttact taacatatat cggaattctg cataaacaga aacatatccg ctgtctaaaa
    12001 taaatccttt gaccgctgat ggaagttttt caccacttgc catcatgatc gtagaagctc
    12061 ccattgatcc gccgtgtaaa ataatggacg cattcggctg ttcatctaag atgagttgga
    12121 tccaggcaat taaatctagc cgatctagcc atcccattcc gatgatttct ccttcacttt
    12181 ctccgtgcgc acgtaaatca ggtacaagaa cgttgaatcc tttctctgca tatcttttac
    12241 ctatatgaga catgtctctt ttccctgtgc ttcggtaatc atgtacgcaa atgacccatt
    12301 tattctgctt atttggatgg atgaacatct gaccagctag tttcaacccg tcataacttt
    12361 tgattgtttt gggtgttccc tgctcccaaa agctttcaca ctcttcttct aatttttgtc
    12421 gttttttttc tactgacgtg actgcagcag ggtccatcaa acgatgacca gtgtcttcat
    12481 accagtcata tcctctctgt actcccgctc ttactaattt ctttgcataa aaataagaaa
    12541 gaaaatggta actgatccct ataattatca gaataagaat gaaataaaaa ataaccattg
    12601 tacacccgcc gctttctgcc aaactataat ctaactatta tccaatgtac cgatccatag
    12661 gattctttat tttcggcaca cgtaaattcc atttcttttt cattgtatca tttacttaaa
    12721 gttctcctca atagatttac ttgtaagaga gcatttacat tcgcaataaa agccagtata
    12781 ctaatttcgt gcattataat caagacgctc gtcttggact ggttcggaaa cttcccagaa
    12841 taaaaaacta agtatctcta aagaaaggag attggaaata atgcgaaaag taattattga
    12901 ttgcgatcca gggatcgacg acactttagc tttatcatta gctgtcaaat cccctgatat
    12961 cgaagtagtt gctattaccg tagtttgtgg gaatgtccct gcagatatag gtacgcagaa
    13021 tgtgctgaaa tgcctggaac gctgtgatcg tttagacatt cctgtttatc aaggaatgga
    13081 aaaacctttg aagcaacctt tcatcagtgc acaagacact catggtatgg atggtttagg
    13141 ggaaacaaat ttcccgatga tcttgagaaa acaggcagaa cctctccatg ccgttgattt
    13201 tttggctgat tatttcaaag aaaagacaaa tacatcagtc attgctttgg gtcctttgac
    13261 caatatcgct tttgctttga aagtcaaccc aaatattggg aaacatatgg atcgttttgt
    13321 ctcgatgggt ggaacttaca aaagccatgg taactgttcg ccagttgctg aatacaatta
    13381 ctggtgtgat cctgaagcag cttcttatgt attcgagaat ctcaaacaaa cgatagaaat
    13441 ggttgggtta gatgtgacaa gagaaatcgt cttgacaccg actatcctcg aatactgctg
    13501 tcaaatgaat cctgaagagg gagagtatct aaaagcgatc actcgttttt attttgactt
    13561 ccactggaaa caagaaagaa ttttaggatg tgtcatcaat gatccgttag ctgttgctta
    13621 ttttatcgag ggggccatct gtgaaggttt caaaagcttc actgctgtgg aaactcaagg
    13681 aataagtcgt ggacaaacat tggttgatcg ttatgaattt tggcagaaac ctgctaacag
    13741 taaaattatg acaaaagtag atactcgtct ttttttccga aagtttttaa ccgttctttt
    13801 aaatgcacaa gaagaaacga ttatgaaaga tttggaaagg ttaaagatgg gctaacaaaa
    13861 tgaataaaaa caaaatggca actcgcgtac ttacagtgat ggctttgtgt attgggatca
    13921 attatattgg aggaacgatt gctttatggc ttcgtttgcc aatctatctc gattctatcg
    13981 gaacgatttt tgctggtgca ttgcttgggc ctgtacctgg aatgctgaca ggattgtcca
    14041 gcggtatttt aagtggagtg actacagata ttttttctct gtattattcg ccaatccaaa
    14101 ttctcacagg cttgcttgct ggattgattt tgccaagata tctcggcaaa gataaaaaca
    14161 gcttgaaact gttattactc gctttgattt tgtcggtacc tggaacgatc gtttcttcga
    14221 tcattaccgt tcagttattt ggcgggatca cctcctcagg atccagtatg atcgttcagc
    14281 tattacatgg gttaggttta agtcagacaa caagtgtgat cttggtacag gcaggtacag
    14341 actaccttga tcgtttgttg tctatcgtcg ttgtttcatt agtcatcact atgttgccac
    14401 aacgcttgat atattcaaaa agaaaaatag gctgaataaa cagagctgga ttagcaaaat
    14461 aatgctaatc tggctctgtt ttttcattta ttaaaaaaat cttacgctaa gtaggataaa
    14521 ataattcctt gatatttttc aatcatatcc aaataattat cgatttcaac atactcatct
    14581 acttgatgag gcaaagtaac gacaccaggc ccaaagacga caaagtcgaa ggaatggtta
    14641 gctttcgtaa attcggctgc atccgttgtt gctgccgctc caactaaagg taatggctgg
    14701 ctgaattgtt gctgaatgca atgaatcaaa ggagaatctg gatcagcttt gacaggtatt
    14761 ttgttgtaat cgatcatcaa ttccaaatga tagtctgtct cttgattcaa ttcattgacg
    14821 attgattgca gcaatgcaat gattttatca tttgggtatt ctggaatcga tcgaatgttc
    14881 ccttggagct gcgcatggct tgggatgctg ttgacttgat tcccgccact gatcaatgta
    14941 acattatgga ttgtttttcc taggacagga ttctcgattg tttcagcgag gtgattcatt
    15001 tcagcatttg ctttcgtaat gaattcattt aggtgattga ttgcattata accttggtct
    15061 ggcatagagc tatgcgcttc ttttccgtgc gaggtgactg tatagttgat tgatcccatg
    15121 tgcgtataca tcaaactgta attagttggc tctccgatga tcaaggcatc taagtcatct
    15181 acatagcctg ctttcgtgag ctgttcccca cctagttcac ccacttcttc tccgacagtg
    15241 gctaataatt tgactgttcc gttaaatggt tttcctgact ctttcagttc gatcatggca
    15301 atcaccattg ctgccaagcc actcttcata tctgtagcac cacgtccgta caaacggttc
    15361 ccatggattt ctgctgcaaa gggtgcatag gtccaagacg attcatcacc agccgccacg
    15421 acatccatat gcccagatag gcctaacact tttcctgatt ggcccttttg gtaacgagca
    15481 atcaaattat ctctaccatc tctgtaagaa acaatctctc ctgttatgtc atgctttgcc
    15541 aaaagcttat tcaaataggc tgctacttca ccttcgtttc cattcactga tttaattcgg
    15601 atgatctctt gtaaaatcgc gattttttct tcttttttca tctaatcctc tcccttgcca
    15661 tttcttttta ttgtatttga gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa
    15721 tgaagaaaga gagccacttt atgaaaaata tgctgctact tccctcctat tttatttata
    15781 cgagaggact tgctcataga ttcctattct actgttctat ccttaaaagc tcttatatga
    15841 tgctagttat tgttagcttt cctttttcaa ggatgatctt ttcttgtacc attctttgta
    15901 aacatcatat acagaagcta gttgcaaaaa aaactctcta cctgctgata tt
//
LOCUS       DANJLF010000057        15779 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000057 DANJLF010000000
VERSION     DANJLF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            48..2066
                     /locus_tag="PWT25_002030"
     CDS             48..2066
                     /locus_tag="PWT25_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1022110.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2136..2342
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2493..4907
                     /locus_tag="PWT25_002031"
     CDS             2493..4907
                     /locus_tag="PWT25_002031"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL1022111.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5255..6808
                     /locus_tag="PWT25_002032"
     CDS             5255..6808
                     /locus_tag="PWT25_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL1022112.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7189..8973)
                     /locus_tag="PWT25_002033"
     CDS             complement(7189..8973)
                     /locus_tag="PWT25_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022113.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8988..10709)
                     /locus_tag="PWT25_002034"
     CDS             complement(8988..10709)
                     /locus_tag="PWT25_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022114.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10809..11570
                     /locus_tag="PWT25_002035"
     CDS             10809..11570
                     /locus_tag="PWT25_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1022115.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            12537..12805
                     /locus_tag="PWT25_002036"
                     /pseudo
     CDS             12537..12805
                     /locus_tag="PWT25_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            13477..>15779
                     /locus_tag="PWT25_002037"
     CDS             13477..>15779
                     /locus_tag="PWT25_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022116.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         5223 a         2749 c         3248 g         4559 t
ORIGIN      
        1 aagttataat tattaataat agataaattg aagttggagg atagaagatg gaaaacattg
       61 caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca aaacgagggg
      121 cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta gtacagccgg
      181 ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat acagcggcat
      241 ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat ctatatgcat
      301 cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg ttatctcaag
      361 caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc gtctacatgg
      421 atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc cgacaatatc
      481 cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact tcatttggca
      541 acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca gatgcgacag
      601 cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta aataatatca
      661 tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca ggcggtggag
      721 cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca agcaattctg
      781 gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct ggtgattctg
      841 tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa tggaacaaca
      901 tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc caggaaaatg
      961 gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct ggtacatcaa
     1021 acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct gtttggaaga
     1081 tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat atcaagaaca
     1141 atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct aatgctggag
     1201 caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc tcaagttctt
     1261 ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt tggagcgtct
     1321 caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc caaaataact
     1381 ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac agcacgtcag
     1441 gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact ggcagcagtt
     1501 cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg gcaaataaaa
     1561 acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac ttcatttatc
     1621 caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca ggttcaacta
     1681 cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca tcatccactg
     1741 gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg aacaaacatc
     1801 atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc atctatccag
     1861 gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa tcagttactg
     1921 gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct gacagtcatg
     1981 gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt atttatccag
     2041 gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga ttgaattcca
     2101 caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa ggaagtagta
     2161 aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat gcttttgaac
     2221 tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga cggccgttac
     2281 aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat tacgtcctat
     2341 aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg agaaaacaaa
     2401 gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt ataatagaaa
     2461 caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa gaaattgaga
     2521 aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat gatcctgaaa
     2581 agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg ctacatgtag
     2641 ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga agtcaaggat
     2701 acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa caatatgcgc
     2761 tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc
     2821 aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca actgatccgg
     2881 agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt ctagcttatg
     2941 aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca aatgaagaag
     3001 tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca atgcgccaat
     3061 ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa ttagttgatt
     3121 ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta ggagcaaata
     3181 ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca cgaccagata
     3241 ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt agagaaatta
     3301 cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct aaaaaatcag
     3361 atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt gcttatgcaa
     3421 tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta ttatcttcat
     3481 acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt gaattcgctc
     3541 aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa aaagctgctt
     3601 acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac aaacaagaag
     3661 caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa gaagtcagct
     3721 atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca atcccaatca
     3781 tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca ttacggttgc
     3841 ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca aatatcgagg
     3901 agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa
     3961 tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct cataacaaaa
     4021 aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc tacattggtg
     4081 gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa ttcttatacg
     4141 atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa ggaatgatct
     4201 taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg
     4261 tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg gggccattag
     4321 atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg
     4381 tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact acattgaatg
     4441 atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa
     4501 atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa gcctataaag
     4561 tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca ccgattgctc
     4621 ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt tcttacgcac
     4681 catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt gttttccaag
     4741 taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa gatgagctag
     4801 aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa
     4861 aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg gctctttgtc
     4921 aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt cattctgtct
     4981 cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt gattttcatg
     5041 ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat ctctgtaatc
     5101 atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct gaaattgaga
     5161 gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg gattttgacg
     5221 cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat ttattatcga
     5281 agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga aaagaataat
     5341 gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag ctgtatcgtt
     5401 cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa aattaaataa
     5461 tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta ttgtttttct
     5521 tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca
     5581 aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact atctttattt
     5641 tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc agttattgct
     5701 gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa
     5761 cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc
     5821 tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc tcattctttt
     5881 agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc atataaatgc
     5941 tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga caaatgaagg
     6001 gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa atgatctcaa
     6061 acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta attgggtatt
     6121 tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa cggggctgat
     6181 tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct catggtttat
     6241 ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta aaacatggca
     6301 aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg tattttatgg
     6361 caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa ctttgtacat
     6421 ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa tgatcaatca
     6481 gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga cacgtgaaag
     6541 tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt cgattccgga
     6601 aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta ctcaaataag
     6661 cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg atgcagggaa
     6721 aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat tcgttcagca
     6781 actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga cgttcagcac
     6841 cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata gtgtaactcg
     6901 tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc tcgtatttta
     6961 caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg
     7021 attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta tccaaaaagt
     7081 atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa aaaagctatc
     7141 gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt acgctatctg
     7201 aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt cagaatgtgt
     7261 tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat tttcaatcgt
     7321 tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt ccattgcttg
     7381 ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta aaataacgac
     7441 aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc cttgagataa
     7501 cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc gaataaagtg
     7561 atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat ccattttccc
     7621 gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta aaaccatccc
     7681 aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt cgaattcgat
     7741 ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg tttttcctgc
     7801 accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat cgacttgatt
     7861 catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt
     7921 cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt catctaatat
     7981 ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca cataagaagc
     8041 ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta agaagccgat
     8101 cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga tgaaggcgag
     8161 ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag cacgtttaaa
     8221 agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag cttgctgatt
     8281 gaatgttttg accactagat taccagctaa gatttcttcc attcgattat ttaaaacacc
     8341 cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct tagttgtaac
     8401 aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt cgatataaaa
     8461 cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat tgatacctgt
     8521 caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga tgtcacctac
     8581 ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga tttcttttcg
     8641 caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt
     8701 gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca atgcttgttt
     8761 gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata agagatcatc
     8821 gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga ataaactgca
     8881 tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa aacgtatcaa
     8941 agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta tattccttgt
     9001 gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg agtaccttgt
     9061 ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat tgtactgagg
     9121 cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc tgctcttaat
     9181 gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata gatctctgct
     9241 ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga ataattagaa
     9301 ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag ttcttctaat
     9361 tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt tcccatctgc
     9421 aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata actgatgtgg
     9481 gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt tacttttcct
     9541 atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc agatccggtc
     9601 ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc ttctagaact
     9661 ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc atttgtctga
     9721 tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat ctctgcttgc
     9781 agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac caataccata
     9841 gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc agtcacgaca
     9901 ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat agaagcataa
     9961 acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg gttcactttg
    10021 atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc atatgattga
    10081 ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt gatggtgatc
    10141 cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat cagtcgataa
    10201 acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa tagagaataa
    10261 cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat catttggcaa
    10321 aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt caaaagagat
    10381 ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg gaaatatttc
    10441 tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact agaggcaact
    10501 gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt ttttataact
    10561 gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt tggtacacct
    10621 aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag tcctttgttt
    10681 ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg atacttttcg
    10741 attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt
    10801 gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt gatttgccaa
    10861 aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga caagaagaga
    10921 atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc gttttttata
    10981 gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca ttgacggagt
    11041 tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg gtgatgaaac
    11101 aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc gatacaaacg
    11161 aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg gagtttgaag
    11221 atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg atgagtatgc
    11281 cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa gtacatttat
    11341 ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga ttcttattga
    11401 aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa agaatgtatg
    11461 aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat acggatgaaa
    11521 acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg
    11581 tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt ctggttcgat
    11641 tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt tttttagagg
    11701 aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga tgtaattgtc
    11761 ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc atggtgaact
    11821 gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag atgagaatga
    11881 taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt acattattac
    11941 aatatgttaa taaggcaaaa ataaatagat ggattgaata aacatatttt ttaatcaaat
    12001 agtccctact gtgactttcc tcagctatcg gtgatactac cgatagcatt tttttgctta
    12061 taaaataatt ataagtattt agagattttt cttctaacat tcttcaaaca agaaaaatca
    12121 caaaggttat tgttattttt atatattctt tttaagaatg attgtttttt tatagaaatt
    12181 ttaaaaaata aaacacgtta ttgatggata gttagtttgt tatttggtaa tattatagaa
    12241 gttgactggt agtgatttat ttttcgtcgt tttataaaag tgttattttt tggaaaaatt
    12301 cacaaatatg taacgaatgt ttttctggaa tttgctgtgt taaaaagcag tatgactaat
    12361 ataaatacta ttgtcattta tgcaattttt tatggatagt aattttagag gggaatcaat
    12421 gaaggaagaa tagaagggaa aaatagttgc aaatacgcca gttagcaaaa atagcaatac
    12481 atcttcaagc tttaaaattc gagagaatca tcttggtagt ggaatcaact acaacaaaag
    12541 gattaaacca attaatagat agaattacca atggaaaaat agaaaaggta gttattctat
    12601 acaaaagttg tttaattcgc ttcggttatg aacttagtga aaccctttgc aatatatggt
    12661 acagaatata gaaattattg acattacaga aaatgatgaa gatttaatac agattgtcac
    12721 tatttgtaga taccttttac aaggaaaccg agccaataaa gctaagaaga tgattaagga
    12781 gttgatggaa gacgatacaa gttaagaaag tgaaactacg cccaaataaa gagcaaaaag
    12841 agtaattgga cgtggaaaat gcaagaaatg aaccatcgat tcggtggaca gtttattagt
    12901 aatgacggtt tgagaacatc catgacgaga atgaaggaac atcctaaatg cgtttagttg
    12961 aaccaagtgc caaataatat ggccaaataa gcgatcagtt atcgatgaat cccaagtatt
    13021 ctaaactata tattcatttt gatgctaagc attgatatat ttttttggga ttgatgaagg
    13081 ttaaccaata cagggattca ctgatgaaat tctagagtct gaccttggta tcaacgattt
    13141 agtgattgtt tctaatggaa aagtataaaa gtatataagt acattaacaa aataactatt
    13201 agggttacgc tccagcttgt gcaaggcact ggcaggagga atcgtgaaat cataattatg
    13261 gttaagccac acggacaact aaaagtcata caaagctatc gaatcaatca acagaagaca
    13321 atcggcaatc attagctttg tttacagctt acattgcaac gcttgagttg aaccgaaaat
    13381 acgagggagg taacgcagcc attttgagtt ggggaatctt tttagaaaat tatcgggtac
    13441 cttgttgttt agtcaaaatt ataaggggga gtaatagtga ggaacaaatt tcataaactg
    13501 ttaagtatgt ttctagttat gcttattgtg atcaatcaat ttgggaacat aacggttcta
    13561 gctgagtctt tagcagaaca gccagagcgt tcgctgacga tcgaagttga tcaagaggag
    13621 gctgctttgg gtgagcagtt ccagttgcga gtcagaggaa caccagacct gctaaatgca
    13681 ttggtcattg ataagagtga ggatttgatt gaaggggaag tgaagcaact caatgattac
    13741 gaacgtctga ttacttattc ttcccagcat tcaggtaaat acgaattgac cgcaagaagc
    13801 ggtgatattc aatcaaatac ggtctcaata gaaatcagtg taccagaaga aagcacaagt
    13861 gagtctttta atgtaccaga agaaagcaca agtgaatctt ttaatatacc agaagaaagc
    13921 ttaagtgaat cttttaatga agccagatca tctgagccat ctataacgca ggaatctcag
    13981 attgaaagac aagaggagct cttgaattat gacatcggca tctctgctga gcgccccact
    14041 ttgccgagca ggcatacaca aacgttaaag ggatacattc aaggaggcgc tggcgatatt
    14101 cttgaagaat atggggagat ggtcgtacga gttcctagag catcgattgg cttaattgag
    14161 ttcattagag tggaggtcgc tgatgattca cctttaaagc cactggcatt ttaccaagaa
    14221 ggcacagacg ttgtttttcc atttaccttt gattatgaag gattaaatga agccgctcag
    14281 tcgtatacat tcagtatcac gtttgaagtt gctttctttc aatcaggcgg tactgagcca
    14341 aataatgact ttcaagtgag tcttacttat gctgagcgag cagcaaacac ggaattttca
    14401 attacgccta ttccttcatc aagacccatt ttttcgaaat tatactatgg ctctagagaa
    14461 atcgacagcc aaggaaatct tgggaatctg ctgattgacg ggcaagacgt gaccaacagt
    14521 aataaattta ttttgcaggt aaattatgaa ggatcacgaa aagacggtgt agtggttacc
    14581 gatgtactac cgcaaggaac aactttggca ccgaatccga ctacatggag ttccactgga
    14641 aatagagaac ctatttcagg tggggttcgg atcgcagaag taacgaatcc aggtgaagca
    14701 aacgttagaa ctcattatgt ttccgaggaa tttgtggaca aagtatcttt taataaggaa
    14761 acgaatacac ttgttgtgga ctttggtaat ctagcagagg gtcaatcgta ttttattgaa
    14821 tatgctcttg aagtgaccga tgtcgacttg ttcaatgaag gaagacaaga ggatgggttt
    14881 tctcggaatc gagcgagtat gacctacaat aatcaggtaa tgagccgaga ctaccgcttg
    14941 agaatccctg aaagtaatct tccggcaatc tctttaagaa aaagtgtgga caaaaatgtt
    15001 atcaatctca atgaaaatga actaacgtat actctgcatg tctcgatgtt ttctggaaca
    15061 agaaatacgc ttgttattga agatgttttg gaagaaggca tggaatttgt gtcatttcta
    15121 tcagatattc cctctttcat tgatgccgaa gtcaataatg gaatagttcg tttcagtgcg
    15181 aataatacga ttggtggtcc gacaagtttt accctctctt ttaaggtcaa tgtagaggcg
    15241 tatgcggtcg gcgatcaaat tttaaacttt gcaacgcttg tgaatagtga caatcgattg
    15301 aatacgagta ctgtaagaac aaggaaaatc gatgggcgta ttaaagtaat taaaactgat
    15361 gagaatggcc aaaggctggc aggagcaaaa tttgaaattt tatcaagcga ttcacaagtg
    15421 atacaagaag gtataacggc agaaaacgga gaatttttaa gtgaaccgct tcctgtaggt
    15481 agctatcaag tccgagaaac agaagcaccg gaagggtatc ttttagatgc gacggtacat
    15541 gatgttttta taaatgaata ctccatagaa gaagtgacgt taaccattga aaacatcctt
    15601 gatacgggtg gtgttgaact aattaaacat gctgaaggca gtggtgaagt ccttcaagga
    15661 gctgtttttg agttacagaa ccgagaagga gaaacgctac aaaccggttt aactacagga
    15721 gaagatggaa aactggcaat tgacggttta gcaccaggag catatcagct ggtagaaac
//
LOCUS       DANJLF010000058        15612 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000058 DANJLF010000000
VERSION     DANJLF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            195..545
                     /locus_tag="PWT25_002038"
     CDS             195..545
                     /locus_tag="PWT25_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL1022117.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            644..1174
                     /locus_tag="PWT25_002039"
     CDS             644..1174
                     /locus_tag="PWT25_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL1022118.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1328..2479
                     /locus_tag="PWT25_002040"
     CDS             1328..2479
                     /locus_tag="PWT25_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL1022119.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2588..4627)
                     /locus_tag="PWT25_002041"
     CDS             complement(2588..4627)
                     /locus_tag="PWT25_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL1022120.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4685..5242)
                     /locus_tag="PWT25_002042"
                     /pseudo
     CDS             complement(4685..5242)
                     /locus_tag="PWT25_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(5781..5987)
                     /locus_tag="PWT25_002043"
     CDS             complement(5781..5987)
                     /locus_tag="PWT25_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022121.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(6003..6617)
                     /locus_tag="PWT25_002044"
     CDS             complement(6003..6617)
                     /locus_tag="PWT25_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL1022122.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6764..7447)
                     /locus_tag="PWT25_002045"
     CDS             complement(6764..7447)
                     /locus_tag="PWT25_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL1022123.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7556..8014)
                     /locus_tag="PWT25_002046"
     CDS             complement(7556..8014)
                     /locus_tag="PWT25_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL1022124.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8098..9861)
                     /locus_tag="PWT25_002047"
     CDS             complement(8098..9861)
                     /locus_tag="PWT25_002047"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL1022125.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(10082..11542)
                     /locus_tag="PWT25_002048"
     CDS             complement(10082..11542)
                     /locus_tag="PWT25_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022126.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11750..12676)
                     /gene="glsA"
                     /locus_tag="PWT25_002049"
     CDS             complement(11750..12676)
                     /gene="glsA"
                     /locus_tag="PWT25_002049"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL1022127.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12699..14126)
                     /locus_tag="PWT25_002050"
     CDS             complement(12699..14126)
                     /locus_tag="PWT25_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1022128.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14157..15371)
                     /locus_tag="PWT25_002051"
     CDS             complement(14157..15371)
                     /locus_tag="PWT25_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL1022129.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4476 a         3113 c         2680 g         5343 t
ORIGIN      
        1 cgactaaatt ttaccaaaag tgagtctctt tttgagttga aacgaagaaa aagtgttagt
       61 agaaaatcgc cttcgatttt ccaccaacac aaaatattat agaacctttg tttttttgaa
      121 taatcgcttt ttcttctctt tttcttatat actataagta gtaaataatt tgaaaaaaag
      181 aaaggtgact gattatggcg caaaatcgta tttttacgac aagttttgca agcgtttatc
      241 ctatgtatgt agcaaaagcg gagagaaaag atcgtataaa agaggaagta gatgaaatca
      301 tttgttggct gacgggttat tcccaaacag aactagaata tcaactacaa aaacaagtga
      361 ctttcgaaca attttttgcg gaagcccctg tccccaatcc ttcacgttcc ttgattacag
      421 gtgttgtttg tggtgtacga gtagaagaca tcgaggataa cttgatgaaa gaaattcgtt
      481 atttagataa aatgatcgat gagctagcaa aaggcaaaaa aatggaaaag atcctcagaa
      541 gttgattttt ccagagatat tcttctcctt cgctcatttt gtaacattta atttggtaaa
      601 atgactagtt cacgttaaaa taagtcaaag gaggttttct tatatgtttg agacgattaa
      661 acaaaatatt caaaaatacg ccttattaag agctgctgtt tatattattt ctgggattgc
      721 gattgtgcta aacccttctg ctgtttttca ttttatcggt tatctgatca ccgcctatct
      781 tgttttgctt gggttgttga atctttttga ggcttaccgt tttaaaagga aaacaggttc
      841 ttggagtatc ggtctgttca gtggtctttt tttcctgatc ttagctctga tcgtctatgc
      901 ttttgcacct gcaatcgttt ctatcctgcc gattcttctt ggattagctg ttatcatcaa
      961 cagcttgttc cagttgttct tcagcatgaa tacaaaagct aaaagttgga gtgtttatag
     1021 tagtattctg ttgttgattg gcggattcgt cttattgttc aatccattca aaagtttgat
     1081 gcttctattc caaatattcg gctttatttt gattttcatg ggcatccagg agattatcaa
     1141 tttcatcaga ataagaaaaa tgactatacg ttaatcattt ttagttatta aaatagaccg
     1201 tataaaaacc aacaatccaa aaaaatcttt tggattgttg gtttttttgt gagtatattt
     1261 tcctattttt tcaataatgt atgttatgct aagttcgggc tatattttat acaaaggaat
     1321 gatgagaatg gaatttattg gtattttatg cctgatattg atttcaacaa cgattggctc
     1381 acatatctcg cggagattag gtatcccagc agtgattgga caattacttg ttggtgtttt
     1441 gctaggccaa gctggattga attgggtcaa acctgatatt ctagtccatg acttttcgga
     1501 gattggtgtg atcttattaa tgtttttagc tggtctggaa agtgatttat ctcttttgaa
     1561 aaaatatttc cgacctggta tgttcgttgc tttgcttggg atcatcttcc cggttgtttt
     1621 tggctggttg acaggcaaag cattccaagt ttcgggaaat gaagcgattt tctttgggat
     1681 tattcttgcg gcaacttctg tcagtatttc agtagaagta ttaaaagaac taaacgtagt
     1741 gaatacaaaa gaaggatcaa caattttggg tgcttctgtg gtagatgaca ttttagtcgt
     1801 tttagttcta agtttcagcc tatcttttct aacgggggaa gcttctagca atgcttcttt
     1861 accgcttgtt ttgattgaac agctaattta ttttgttttt attttcttat tagtcaaatg
     1921 gattgcgcct ttcctacttt catttgcaga aaaagtgtac gcaaattctt ctattatcat
     1981 cgtatcgcta gttatttgtt taggaatgtc ttacgtagcg gatcttgttg ggcttagctc
     2041 tgtcattggt gctttttttg ctgggatagc tgtcagtcag acaaaggtta aagaagaagt
     2101 ataccacaat gtagaggctt taggatatgc agtatttata ccagtatttt ttgtcagtgt
     2161 aggattagaa gttgattttt ctgactttag tgaacaacta ctctttattt ctatattgac
     2221 gcttgtagcg attttgacca aattgcttgg aggttatttt ggtgctcaga ttgccggttt
     2281 ttctcaacat agttcattga tggtcggtgc tggtatgatt tcgcgtggag aaatggcttt
     2341 gatcatttta caaattggac agcaaagtca attgatcgaa ccacagtact attcaccttt
     2401 agttatcgtg attttattaa gtacacttat ttctccactt gttctcaaat attttacaaa
     2461 aaaagtttat gaagtgtgag taaatcaaaa agaagttgtg aaagagacat cgtgaaaacc
     2521 gtttatttga aaaaaggact gtgacgataa cgccacagtc cttttactgt acttaaaaag
     2581 caatttttta ttgataattt tggttgaggt attgcaatag ttccgatgct cgtttggttg
     2641 ctgaatcatc atcttcttta ttttccaaca tgctgaccat catataacct tggttatcaa
     2701 gattgaaagc aaacaagaaa ctgttctctt gcccctttac atcctgtttt tctttgattt
     2761 ccgctgtacc agttttcgct gccaacggaa tccctaaagc agaaagagaa tgtgctgtac
     2821 catttacatc ttgcacaact tctctcagat ctggcacgat cgtttgtact gctgtttcgc
     2881 tgatgacatt cttcttatct tttgtctctt tatctgcaat caatttagga tagacaagtg
     2941 tgccattgtt ggtaaaaaca gaatacattg ctgcttgctg gataggagta attagaagtt
     3001 cgccctgtcc atatccagta tcagctagca agatatctga gttaaagcta tcttcattag
     3061 aaatttgtgc tggattcata ctaattggca aatcaaggtc ttcaccaaaa atgaatttat
     3121 ctaagcctgt acgaaatttt ttctcaccca ttttcaacgt ttcttgtgcc gcatatatat
     3181 tatcggaata gatcaaagca gtttttaagt ctacttgtga tacatcacta ctgacacgcg
     3241 ttgttttaaa agatccccaa gaactgtctt tttgccattt aagcccgttg atcgtcaaca
     3301 cttcatttgg atcgatagtg ccgttgtcga gaccgattgc tgctgtaatc attttaaacg
     3361 tagatccagg agcataacct gtcgcaaatc ggctgatgaa tggatgttca ggattttctt
     3421 cataagcttt gtaatcttct tgtgagatcc cgtttgtcat tttgtttgga tcatagcttg
     3481 gagagctagc aagcgcaaga agatcaccgg tttttggcgt tgtcgcaaca gttgatgcag
     3541 cttttcctcc tagactgtca aaagctattt tttgtgcctt cgcatcgatt gtcaatttga
     3601 tatctttgcc attttgaact tcatgttcga tcagaacttt tttctctaca ccgtctgcat
     3661 ctgtaatgct taatttccca cctgttgtcc cacgaagatc cttatcaaaa gccatctcca
     3721 aacctgagcg accgattttt ccattactgc ttaattctgg attcttatca atatcttctg
     3781 ctgtgatatc tcccacgtaa ccaatcagct gagcagctgc ttcacccaaa ggataatatc
     3841 tgccgtctac ttcttgaatg gtagctccag ctggaagttc tggcgttgct ccatcaatga
     3901 ttttcaatgg gacaaagtaa tcgggttgta cccagctttg tgaaatcgcc tgattgatag
     3961 catcttctgt taagtcgaag gaggaagcaa tcgctttgat attggctgtt ttttcgcctc
     4021 catctccaag tttacttgga acgactccta attgttttag tttgcctgtt gttgctaatg
     4081 gttcaccatt tcgatctata atattccctc ttgctgcttc ttgtgtggtc aggcttactt
     4141 tgtcatttcc ttccatttct ggaaaaacca agttgggttg ccagttgatc gtggtttgac
     4201 catcatttct gtctaatgtt cctttataag aaagatcttt caattcacct aatgaggtat
     4261 tcatctttgc tttatatgaa aaactgtaag tagaatcgtc ttttttatct acttttagat
     4321 tcgatatctg aagtcctttg acatcagcag caccgtatat attctggtat ttatctaaga
     4381 tctctttttc agataatgca cttttatttg ccgccttttt tgatgtcatt tctgcagctt
     4441 tgttataatc tcccttgttc aaagcttgga caaattgctc aactgtcttt tctccagctt
     4501 ctactgcttg ggtttcttga tagtgctggt agtaaaaata accgccaccc gcagctgcta
     4561 ttaagatcac tgcgccggca atataagcgc ctgttcgatt tttgccgtgc ttgtcacttc
     4621 ttttcatttt ttcctccttc tcttgcttgt cattccttta tttcactatt tatacctgtt
     4681 tattctaaca tatgtctttg aggaaatcca taccacaatc cagaagcaca ctttctggaa
     4741 ggttcgtttg gatactacca agtaatttcc caaccgcttt gggtagcttg aatattgaag
     4801 ttacgtcttt cagctgctga gagatagctt tgatcacttg ctgctgcctt ctgatcctcc
     4861 cgaagtctcc ttctttgtct tcccgaaatc gagcatactg gagtaaagta ttaccatcca
     4921 tcacttgctc tcctttgttt atatatacgc catctaaatc tagagatttt tctgcatcga
     4981 tcttgactcc accaggaaac agttcattaa cgcaatcgat aaaatcttga aatgtgatac
     5041 ttgcataata gggagcttca aatttgaaat tttcctttaa tgtttgattt aataaatcaa
     5101 ctcccccata agagtaagct gcatttatct tattcattcc gtaacctgga atatcgacat
     5161 atgaatctct catgatagag atcagttttg cttgttgtgt tttctggttg tattgtgcaa
     5221 ccagaagtga atccgagcga cctccaccaa tcgatctttt ttcaccgcat ctcctcctca
     5281 cacagcaatc cacttgtcag gtgaggagcg agtttgtctc atcaaataag tcagtcacaa
     5341 aaatataaag aaaaggtcag aaaccttttc gaacaagaat tctgacctag catcatctca
     5401 tcgtccgagt tttagcacta tacaacgtaa agaaaacatt cttagctatt ggaaaaagcc
     5461 ttaatccaac agtttctgtt ttacccattg gcgactttcg acgtttctgg gcaacagcct
     5521 atgtttgtat aggagcctca cctaacaagg ttattactat attctattta cgagttcatg
     5581 atagactttt cctaatgatt aggcaatgga atttgaaatg aaatagtgaa atttaacaat
     5641 ctttaatatt ttttagaaaa aaataacaaa taagttgaaa gaatcccgtt gcaaattctt
     5701 tcagttgcac caggattctt tgataatttt ttagttttat gtgttttttt atatgttttt
     5761 tcacttttcg gaataaatat ttatttgtta aagtaattaa acaatccgct aatcgtttga
     5821 tctggcattt ctatttcctt accatctaaa gtaacagtag taacgacaaa atggtcttgc
     5881 aatggcggaa cttctaagta ctgcccttgc agaaaatcat tcactgtttc atattctttt
     5941 ttcatttctt ttgaatctgt cgatggttta tagcttattt ttaacataac tagtgctcct
     6001 ttttattcgg gacgtgcaat cgtgaaaatt tctcctattg ctgcataatc atttcctgcc
     6061 aaacggctta cagcattcaa ttttcttgga tcgatccttc ctgtcttatc atcatagact
     6121 gtttcattta aatgatattt ctgtacttct agcaaaaata aatcagctgt tactttattt
     6181 ttattccgta tctcgacatg ttcatataag gatgtttcaa aacgaacttt tgcttctgtc
     6241 aatgctggta ctgaaatgat tactgaatca gttaaagtca agtcagtcag gtacagttcg
     6301 ctttcctcat taggcagatt ggctgctgtt ttatttactt cttctacgtt gtcctcatca
     6361 aggatatgga taacagcttc tcttgtctca agaatattct ttgctgtgtc ttttactgca
     6421 tcttgttttc tctggatcgc caaggataag atgggtcgat tcgaactgac gatattgaag
     6481 taactgaagg gtgcgatatt cactatatct tttgctgatt tagtcgttac tactgctact
     6541 ggtctaggga tgactgaacc aatcagcaac ttgtaatttt cacgttctgt caattctgaa
     6601 ggactccgtt caaacatggt ttcgccttct ttctataaaa ttcgttaaaa agcagatctt
     6661 gaagacaaaa tgaaaagtaa tgcttttttc atcttcttag aatctgcttt aaactatcta
     6721 tcaatttttc ttttttgtca agaatctgtg ccgtgcgtgt tttttagtta tttgctttac
     6781 ttgcggcttc gacatatctt ttagtgatca attgcggacg ggtgataata gatccaacta
     6841 cgactgaaaa agcacctaat tcatatgctt tctctacttg ttcaggagag tcagtatgtc
     6901 cttccataat tactgggata tcgacttgtt ctatgatttg attcaataaa tcaaaatcag
     6961 gtgtctgcgt ctttcgtgaa tattctgtat agcctgctaa agtcgtagac aaaagatcaa
     7021 aaccatcttc ttgcgcttga agcgcttcct caaaagtcga aatatctgcc atagctaacc
     7081 gaccgtgttc gtgaatcatt tggacaagtt cagcagttgt tttttctgtc ggacgtttac
     7141 gagaagttgc atccagtgca atgatttctg cttgcgtctc caaaagttct tccacttctt
     7201 tttctgtcgc agtaataaac acttctgacc catcgtaatc tcttttgata atccctatta
     7261 ccggtaaatc aacgattcgc ttgatttcat tgatatcttc tgtcgtattt gctcgaatac
     7321 caactgctcc gccttctttt gcagccaaag ccattctccc cataataaaa gaactgtgta
     7381 gcggctcgtt ttccaaagct tgacaagaaa cgacgactcc gcctttgatt tgttccagtg
     7441 tatccacaat atctcctcca gtaatgttat ataaaaatac agcttcatat aaaaatgtaa
     7501 cccttttcta ttcaggagac aataatgagc tatttctttc agcagacctt tcttactaaa
     7561 agaaaattac ttcaaattct tcgtatacga ccagcttctc ttctgtaaat tctatgttca
     7621 acgcttcttt ataagcatgt ttaaaaaact cagcatggac tttccgccaa taagctaatg
     7681 tccgatcccc ttctccttct ttccaagcat gctctttcgt gacttcacta aaaggcaaga
     7741 tggtcacatt tatatttttg atgatacaaa ctggttcatt ttttccattc aggaccacgt
     7801 ctaattgacc aacttttggt aaaggctcat tctcttgttt atacagatcg taaccacttg
     7861 atgtaccagt ctttatcttt tttacaacta gttcgctcaa ctcgtctgcc attttttctg
     7921 tatccccaaa tgaaaagaaa cctaattttt ctgattgata actaggatat ttttcttgga
     7981 actttttgac atattcttct acttgctgat acataacatc cacccctttg ctttttataa
     8041 tgaaaaaacc atccagatac tagtataaac aattatctgg atggtgagca taatgaatta
     8101 ttgaatattc aagacacgca gactatcttc aacgatttgt tttggtgtta aatgataacg
     8161 ttcataaagc tgccctacag gaacgctatc cgtgaattct ttctctgcac caaaatttaa
     8221 tacgttcatt gaagaatcac cataataacg actgatattt tcgccaaatc ctcctgacaa
     8281 actaccatct tctaatgtga tgactaattc gtgtgtttct tttaatttat ctaatgtttc
     8341 tacgtcattt tctgtgatcg ttcctggatt gatcaacgtt gcttcgattc ctttttttct
     8401 aaaagctttt actgtttctt ttcctaattc aagaaaaccg cctaaagcta aaattgctac
     8461 ttttcctcca ttatttactg tttgataggc tggaatacta taatcatttg cgatagctgt
     8521 accatgttct acaccatgtg taggaattcg gattgctaca ggctgcgctg tttgttcgaa
     8581 cgcccaatct agcatagaga tcatatcctc ttttgttgct ggagcaagat atttgatgtt
     8641 cgggatactt gataagtaag gaatgtcaaa tgttccttgg tgtgttcggt cactagctga
     8701 gatcgttccg ccaccgacta aaataatcac tggattctcg ttgatcgcta aatcatgtga
     8761 taattgatca tatgcccgtt gcaagaaagt acttgattgg aagacgactg gacgtactcc
     8821 ctgagaagcc aaagcagaag cgaaagtgat agagtgctgt tcagcgatcc ccacgtcaaa
     8881 ataatgatct ggatgttttt cttggaatgt tttcagtcca aaggcgcctg gtatcgctgc
     8941 attgactgcg gcaataggag ctgtacttgt ttgcattttc ttatccaaat aatccatgat
     9001 gatcatatta aatgtttctc cagaagttac ttgctttgcc tccccggtgg acagatcaaa
     9061 tggtacatgc caatgatatt tcattttatt atcgattgcc ggctgataac cgcgaccttt
     9121 ttccgtatgg acgtgaagta caatcggatg atcaatatct tttacttctt caaataaacg
     9181 gatcaccgtt tccagatcgt ttccattatc cacgtaacga taatcaaatc ccattgcttt
     9241 aaataggtta ttgctgcttt taccgttggt ttgacgaagc tcagctaagt ttttatataa
     9301 actgccatga ttctcatcaa tagacatctc attatcgttt actaatacga ttagattgga
     9361 ttgtaaaaca ccaccattgt tcaatccttc aaaagctaag ccaccactta gggagccgtc
     9421 accgataaat gctaccacat ttcctgattc tttcttcaga tctctcgctt tagctaaacc
     9481 tgttgctaag ctgatagatg tagatgtgtg ccccactgta aaatagtcat gtttactttc
     9541 atgttggttg gaatatccgc tcacttcacg gtaatgtcct ttttcaaaac cattttttcg
     9601 accagttaaa agcttatgac cataagactg atgggaaaca tcccaaacga ttttatcctt
     9661 tggtgaatca aatacataat gaaacgcaat cgttaattct actacaccta gattcggacc
     9721 aacgtgccca cctacatcgc tgtccttttg caagattttt tggcgcatct cttctgccaa
     9781 ctgtttcatt tcctcaacag ataatttttt gacatctgcg gggctattga ttgcatccaa
     9841 tacatgtaat actgtactca taatacattc tctcctccgt actatcttct tttaatgaaa
     9901 atagatttgt tacactttat ctattctact acttagagtt gactctaagt caatagcatt
     9961 ttaaaaaaat ttcagagaaa gcttgttata taagtagatc ttcgcacgaa tcaggactca
    10021 aataaaaacc ttctacagac aattaaaaag ctttgtagaa ggtctcctaa ttaaggtctc
    10081 atcaagaaat atgttttttt aataggtgaa gttcctgagc aatatgatac aaatggatgt
    10141 ttgcaccttt taatgagatt ggatagatct ttgtcttttc agtttctaat ccattttgat
    10201 agctagcagc atcgcacaaa accactcttt ttttcgcaca tagacattca tcaggcaatt
    10261 cttctaatga acgaaataaa ataggattta tttcagcgat gccttcttcc gcatacaaat
    10321 gaaattgggt tcgataaaaa aattgtttga ctgaactttc tgaaacaaca aaaatttcta
    10381 tttcctgtct ccccgcttgt tcactgttga tcaaagaaag acttttgacg aattttcgaa
    10441 tgaaattttc attccatctt agttggattt gatttttctt tttccactct tccaatattt
    10501 ctacaatttt tccgtacaga agttgttctt tttctgatag aagatagatc ctttccggta
    10561 aaaatacttg tccatctgcc cacaacgtac ggacaaattg taaaaaggat tgcttgaaaa
    10621 taaaattttc tgcatcgtcg atttcagcga tactaataat ctgtaaaatc aattcattca
    10681 gtgataggtc attgagaaca aaatttttat aaacaacttc gaaatctttt cttaacagct
    10741 ccatgttctc attggtataa tttctactgt tgaacaaaga aaaaatataa tagacatctt
    10801 cttcttgtaa gtctagaatt cctttttgct cttccaatcc ttgacagact aattgaaata
    10861 acggcttcgt ttctgcgctt ttcttatctt cttctttcag tccaagggga aatgcctttc
    10921 ttccgattgc gatctctatt cctcgtatga taaaggactt ttcatcttcc gaatatcttc
    10981 ttttaaaaaa atgagtctct acataatcaa tcaactcgtt acaagcttct tctacatacg
    11041 gatttttatt ttcatagcct tcccaattga cataacgtac taaagctagc aagatgttcc
    11101 gcgagtcaaa ttctgggata atgatttctt tatttttcaa ataatcattt tctttaaaaa
    11161 aatccaatac gatttttttg attgtatata ctttgctcaa agaaataaat tcaagatcag
    11221 ccagttctgt actcgtgaat ataccattaa aataataact taaaaacttt aaaaaataag
    11281 attgaccgta cagaaactgt gtgagctcag aaagagcaat accatgtttg aatacgagtt
    11341 tatacttatg cttctctctt acttgttctt ttatacaata ttctagacac tcggctagac
    11401 tttctagatc gtgaaggatc gttggtgtag tcacccttag agaaatagct gtttgttcga
    11461 tgtctatttc ttggaattca aataattgtt cacacagata cacttttcta aatatatttc
    11521 gctcgatgta attttccagc attcctgata cacgcctcca gtttttctta tgattttatt
    11581 taatcatatc tgtaaatcgt atttttgttt cctttttatt tagaaaagcc ctttcaaaaa
    11641 ccaataaaaa aagaaactgg gacaaaagtc aaacaagctc acaatttcag cctcattgta
    11701 ggtagctttg agccgtttgg acttttaccc cagcctcttt caagaatggt tattcaaaga
    11761 tgtcagcgtc tagttctttg actacatctt tcaacaattg gataccagct gcgctgttac
    11821 caaatggatc tagtgccggg ctgaagacac ctatgccgta acgatttggc acagctgcca
    11881 tcaacccgcc gcctacaccg ctttttgcag gtacaccaac atggacggaa aactctcctg
    11941 attcatcata caaaccagca gttgtcataa ttgatttgac gattgtagca ctttcttcag
    12001 taatgatttg tttgccattc caaggagcaa ttcctttatt tgccagcaca gcagctaatt
    12061 ttgctatgcc tgtagcggtg acattgaggg agcattgtct gaaataagca tcgagtatct
    12121 ccggtacgtc tccttcgatc atttgattac ctttcatata gtaagcaaga gagcgattga
    12181 tatcacctgt tcttgattca gaatgataga tttcttcatt caatgtgata ttaggatcat
    12241 cacagatctc tttcataaat gacaagattc gctcaaattt ttcatctgca tctttaccgg
    12301 caatcaacga ggttgttgca atcgctcctg cattaacaaa aggattcatt ggatgtttgc
    12361 gatgattgat ttccatattc atgatagaat tgaaggcaaa accggttggt tccgtgttga
    12421 ttgtttgaaa gactttttct attccgttgt ctttgattgc taataacaat acgggaactt
    12481 ttgacatgct ttcgatggca aagcgcacat cggcgtctcc tccctcaatc agttgttttg
    12541 tatccaaatc ataaatcgcc atacctaatt gtttgggatc cacttcagct aatgctggaa
    12601 tgtaatccgc cacttgtcct gtttgataat agtggaaatt ttttccaatc aattgttcta
    12661 attcttttac ttccatttga ataaccagct ttcatttttt atttttctgc atgctggaat
    12721 ttatgacggc atgcgtagat cgtaaatgga ataactaaga cgataagata agaaacaatc
    12781 agaatcgttt cataaatccc atgctgtcca gctgcgatac tagcaggtgg aacaaatgag
    12841 atacaaaaag caaaaagtga agtcaaaaga cctaaacaag caacgattac ttttacaact
    12901 tttccgcctg gaacctgata ggctctttca agactgtcat ctttaaaaac cagcttcaaa
    12961 tagcccgcga aaaacataaa gtacaacatc aggtagatta caacagtcaa tgacatcgct
    13021 gcaagaaatg aaagattatt ccccccgccg cttaatgtaa agacggctgc ccagattgtt
    13081 gcaacgatcc catttgcgat catcaaatga gcaggaacac cgtgtgtgtt ttcttttgca
    13141 aaatattcag gaagtaatcc ttcttctgct gctacgtgaa gtgcttttga aggagaggtt
    13201 acccaagaag caatttctcc gatgacacct aaacaaacaa gaatcgcaag aattttcgca
    13261 atccatgcaa gatttggatc aatgtgtctt aataggaatt ctaacgattg aatcacccct
    13321 gtattaagag acaaaccatg ttgaggtact gtagtagcga cggtcaatcc gccaaaagta
    13381 tctaaaatga ttgccagaat aaccaataca aacattgcta atggataatc ctttttaggg
    13441 ttttccattt catttgcatg actggcagat gtttctacgc ccatatatgc taaaataaag
    13501 gaaacaaaaa caaccagcgt aggcaatttc gaaaaatcag gaatcaatgt tttcatagac
    13561 agtatcgttt gtagtggagc gcctgaagcg acataatgga tccctaagcc aaacaagact
    13621 aatgctggca acaagatacc agctacaaaa ccgatccgag caatcaatga agtgtatttg
    13681 gttcctttta actgtgagag taaaatagcc cagaaaataa tcaataggat tgccaatttc
    13741 agatatttat tgttttgtat agcaccaata cccgttgcat aggataatgc accagttaag
    13801 aaatacagca tggtcaagta gccaactgtt atctcaaacc attggaaaaa gattgcggca
    13861 aaaccgaatc gttttcccaa tgtgcgactg acccaagtat agacaccgcc tttttcccat
    13921 cctttcaccg ttgccatctc tgctgcgcac aatgccacag ggataaacca tagcaaaccg
    13981 cctaataaca agaaaaatac taagctgaag cctgaggtcg cgaatgtggg atattcatat
    14041 aatgaaacga ccattgagat ggtcatagcc aaaaagccaa agaacgttaa tttttttgca
    14101 ggaattttcg ttttgctttg ttgcatgaat cttcctcttt tctatttgaa tttgtttcaa
    14161 ttgagtttgt tgccaaagaa gaaaaagtcc aatccttctt cttgttcttc ttttgtagta
    14221 gtgatcgttg taaagtgcat cgccgatttt aaaatcactg ttgtcagcac ataggcatac
    14281 acggcagtaa tgatgacacc aatcacttga atccctaaga agtaccagct gcttggattc
    14341 gttagtaaat ggctgttaga tgcaaagatc cctatcagaa tcgtacctgt gaatccgccc
    14401 atgccatgaa caccccacac atcaagtgca tcatcccatt gtttcttttt acgaaaatca
    14461 acagcagcat gacagacgat acccgcaatg atcccaatca gcatggcact ttttgcttct
    14521 acataaccgg cacaaggcgt aatggctgcc aatccagcaa cggatcctgt tagtacgtcg
    14581 acaaaatcaa agccatttcc ttttactttt gcaatgatca gccaagtaat catcgcaaat
    14641 gcaagagcga tgaatgttga accaaatgca ttgactgctt gttcatctgc tcgaagtgca
    14701 ccaccggaat taaaaccgaa ccagccaaac caaagcagtc ctgtaccgat agcagctgcc
    14761 ataagactgc tatgctgact tctttctcct aaaggaatct ttctttgacc aagtgtgagg
    14821 atacatgcta aagagccgaa tccagctgtc gtgtggatga ctgctccacc agcgaaatct
    14881 ctaaatccca tctgatccaa aaatccgcca ccccatatcc agtggcaaac agggaaataa
    14941 atcagcagat tccaaaaaat aacaagtata atatagcctt ttaaattcaa tctccctgca
    15001 aaagctcctg tcatcaaagg aacggtaatc acgataaaca tcagttggta aagaaagaac
    15061 attaaaaatg gaatcgttgc accatgaaac atgttgggga agcttgtaac atgtctcatc
    15121 aaaaagaaat ctgccgggtt tccaataaca ccgccgatat cccgtccaaa ggccaatccg
    15181 aatccaccaa atacccacat aatcgttgtt atcccaacgg agagaaaaga ttgagacatg
    15241 atcgttagta tattattctt ccgtgctaat ccgccgtaaa aaaaagctag accaggagtc
    15301 atcaggcaaa ccattgctgt acataaaatc atgaatgctg tatctcctgt attgataggt
    15361 aatgtattca tttttttcgc ctcctttcta cgattacgag tataaaagaa ggtgtagttg
    15421 tttttttctt ctgttttttt gaattaatac ttgcatttat aaaagacaga caattaataa
    15481 aagcttgatg tgttgtgctc tttttcgttt ttcacacaaa taaagacaaa aaataaacgg
    15541 tcttcttttt atagaaaacc gtttattttc gtcttttttt atttttaaag atattttttc
    15601 ataaggaata aa
//
LOCUS       DANJLF010000059        15230 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000059 DANJLF010000000
VERSION     DANJLF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            278..787
                     /locus_tag="PWT25_002052"
     CDS             278..787
                     /locus_tag="PWT25_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1022130.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="PWT25_002053"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="PWT25_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL1022131.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="PWT25_002054"
     CDS             2322..3302
                     /locus_tag="PWT25_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1022132.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="PWT25_002055"
     CDS             3424..4842
                     /locus_tag="PWT25_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL1022133.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="PWT25_002056"
     CDS             4913..6382
                     /locus_tag="PWT25_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1022134.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="PWT25_002057"
     CDS             6392..6859
                     /locus_tag="PWT25_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1022135.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="PWT25_002058"
     CDS             6895..7767
                     /locus_tag="PWT25_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1022136.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="PWT25_002059"
     CDS             7848..10382
                     /locus_tag="PWT25_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1022137.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="PWT25_002060"
     CDS             10538..11311
                     /locus_tag="PWT25_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1022138.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="PWT25_002061"
     CDS             11304..12095
                     /locus_tag="PWT25_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1022139.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="PWT25_002062"
     CDS             12105..12596
                     /locus_tag="PWT25_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1022140.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="PWT25_002063"
     CDS             12614..13024
                     /locus_tag="PWT25_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1022141.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="PWT25_002064"
     CDS             13028..14428
                     /locus_tag="PWT25_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL1022142.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="PWT25_002065"
     CDS             14451..14825
                     /locus_tag="PWT25_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1022143.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DANJLF010000060        14815 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000060 DANJLF010000000
VERSION     DANJLF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(296..1024)
                     /locus_tag="PWT25_002066"
     CDS             complement(296..1024)
                     /locus_tag="PWT25_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL1022144.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="PWT25_002067"
     CDS             complement(1002..2576)
                     /locus_tag="PWT25_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL1022145.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="PWT25_002068"
     CDS             2805..3743
                     /locus_tag="PWT25_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1022146.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="PWT25_002069"
     CDS             3755..4666
                     /locus_tag="PWT25_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1022147.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="PWT25_002070"
     CDS             4690..6162
                     /locus_tag="PWT25_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1022148.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="PWT25_002071"
     CDS             6213..6824
                     /locus_tag="PWT25_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL1022149.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="PWT25_002072"
     CDS             6854..8767
                     /locus_tag="PWT25_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDL1022150.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="PWT25_002073"
     CDS             8895..11876
                     /locus_tag="PWT25_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDL1022151.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="PWT25_002074"
     CDS             11873..13534
                     /locus_tag="PWT25_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDL1022152.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="PWT25_002075"
     CDS             13574..14506
                     /locus_tag="PWT25_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDL1022153.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DANJLF010000061        14562 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000061 DANJLF010000000
VERSION     DANJLF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(367..729)
                     /locus_tag="PWT25_002076"
     CDS             complement(367..729)
                     /locus_tag="PWT25_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022154.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(803..1183)
                     /locus_tag="PWT25_002077"
     CDS             complement(803..1183)
                     /locus_tag="PWT25_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL1022155.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1247..2440
                     /locus_tag="PWT25_002078"
     CDS             1247..2440
                     /locus_tag="PWT25_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1022156.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2517..3101)
                     /locus_tag="PWT25_002079"
     CDS             complement(2517..3101)
                     /locus_tag="PWT25_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1022157.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3302..4300
                     /locus_tag="PWT25_002080"
     CDS             3302..4300
                     /locus_tag="PWT25_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1022158.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4412..4921
                     /locus_tag="PWT25_002081"
     CDS             4412..4921
                     /locus_tag="PWT25_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL1022159.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4951..5610)
                     /locus_tag="PWT25_002082"
     CDS             complement(4951..5610)
                     /locus_tag="PWT25_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL1022160.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5612..6376)
                     /locus_tag="PWT25_002083"
     CDS             complement(5612..6376)
                     /locus_tag="PWT25_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL1022161.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6384..7061)
                     /locus_tag="PWT25_002084"
     CDS             complement(6384..7061)
                     /locus_tag="PWT25_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL1022162.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7098..8483)
                     /locus_tag="PWT25_002085"
     CDS             complement(7098..8483)
                     /locus_tag="PWT25_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1022163.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8693..8777)
                     /gene="ffs"
                     /locus_tag="PWT25_002086"
     ncRNA           complement(8693..8777)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWT25_002086"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8800..9315)
                     /gene="tadA"
                     /locus_tag="PWT25_002087"
     CDS             complement(8800..9315)
                     /gene="tadA"
                     /locus_tag="PWT25_002087"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL1022164.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9422..10279
                     /locus_tag="PWT25_002088"
     CDS             9422..10279
                     /locus_tag="PWT25_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022165.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10360..10695)
                     /locus_tag="PWT25_002089"
     CDS             complement(10360..10695)
                     /locus_tag="PWT25_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022166.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10762..12132)
                     /locus_tag="PWT25_002090"
     CDS             complement(10762..12132)
                     /locus_tag="PWT25_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL1022167.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12210..12746)
                     /locus_tag="PWT25_002091"
     CDS             complement(12210..12746)
                     /locus_tag="PWT25_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1022168.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13055..14536
                     /locus_tag="PWT25_002092"
     CDS             13055..14536
                     /locus_tag="PWT25_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022169.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4485 a         2897 c         2366 g         4814 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
       61 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      121 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      181 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      241 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      301 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      361 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      421 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      481 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      541 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      601 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      661 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      721 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      781 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
      841 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
      901 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
      961 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1021 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1081 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1141 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1201 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1261 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1321 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1381 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1441 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1501 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1561 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1621 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1681 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1741 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1801 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     1861 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     1921 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     1981 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2041 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2101 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2161 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2221 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2281 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2341 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2401 acttaatcga atcaagtatg ctttagagac aatcgattaa atcatttatt caaaataaaa
     2461 ggacgagacc ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat
     2521 tctgaaattt caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt
     2581 tgtacatttg cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta
     2641 tgtcggaaat ctagccaagg agttccgcca cctttatacg cttcaatgat ttctgatggg
     2701 aatccagcac cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata
     2761 aagaattggc ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg
     2821 ttgaacagtt cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc
     2881 attcctgttc cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa
     2941 atcacgccat cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt
     3001 gctagttcag ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt
     3061 ggtccttttt cactttttaa atctagttgt gggaatgcca tattgtttcc tcctaatatt
     3121 tgcatacttc ataataataa taacataata cctaacctct cgtaaaaata ctagatttgt
     3181 tttcttttat ttttcatcac tagatacatt ctataactta ccgtttcgat ttcgaattca
     3241 aacatttttg agaagcattc tcaattaaat tctgctatag tgtgaaaagg gaggaatttt
     3301 tatggaaatc tatgacatta caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt
     3361 ttacgcagga atgcgccaag ctaaaacaaa aatcattgac agtctacctc aacttggtgg
     3421 tcaattagca actttgtatc ccgaaaaata tatttacgat attcctgggt atccagcaat
     3481 aaaagcaagt gaattaatcg atcaattaga aaaacaatta actactttta accatacatt
     3541 ccatttaaaa gaagaagttc tctcgcttac tcgtgaagac gaagttatcg agattactac
     3601 aaataaaggc atccattatt ccaaagcagt cattttagct ttaggaaatg gttcattcca
     3661 gccaagaaaa ttgaatttag ataatgctga atcatttgaa aaccacggat tggattattt
     3721 cgtgaatgat ttgatgaagt atgctggaaa aaaagtcgct attgctggag gtggtgattc
     3781 tgctatcgat tggacgttga tgttagagcc tattgcaagc gaagtttact tgattcatcg
     3841 tcgcccggaa tttcgtgcgc acgaacatag tgtatcacgc ttgaaatctt catctgtcaa
     3901 cttgcttact ccctatctca tcgatggatt atcaggcaat aatggggaac tgacagatat
     3961 ccggttaaaa aaagtgaaaa gtgacgagac gattgatttg atgattgatt ctctgatcgt
     4021 caattatgga ttctcttcta atttagaaca tctttcttct tggggacttg atagtaccag
     4081 aaatgccatt actgtaaaat cagatatgtc tacttctatt ccaggcgttt atgcagctgg
     4141 tgacatctgt acctatgaag gaaaagtgaa actgattgct actgggctcg gtgaagcacc
     4201 tactgcagtg aacaacgctt tacactatat taatccaaag gaacgaactc agcctggtca
     4261 cagcacaagc ttatacgata aaaatcttcg tcctagttga ttttttatat ctctttgcat
     4321 ttcaagttaa acccgaaagc agtttcttgc atttttttca tgttccgtta acatgaaata
     4381 gagaaagaaa aaataaaggg ggagcaataa tatggtcgtc aaaactgaga ctttagaaga
     4441 aaaggcaaga gaactattga aggaacgtgg cgtaactgta gaagatatcg cacaacttgt
     4501 catgttttta caaaaagact atatttctga tttgactttg gcggattgta ctgaaagtgt
     4561 caacagcgtg ctaacaaagc gtgaagtaca taacgccatt atcactggaa tccaattaga
     4621 tatattagca gaaaagaaaa aattgatgag tccgttgcaa catattatcg aagatgacga
     4681 aggcctttac ggtatcgatg agatcctagc cttgtcaatc gtgaatgttt acggtacgat
     4741 cggctttacg aattatggtt atatcgacaa ggtgaaacct ggtattttaa aagaactaaa
     4801 cagccacgat ggcgttcatg tccatacctt cttagatgat atcgtcggag cgatcgccgc
     4861 tgctgctgcc agtcgattag cacacaaaga accagaaaaa agaagtcgcc ttactcaata
     4921 attcaacaaa aaaagaaaga aaacgtctat ttagacgagt tctttctttt ttgtttgttt
     4981 caagctattt tttcctaaga aaagaaaaac agcaaaaaat aattctaata atacaaaaaa
     5041 taagataaag ctatgcatga aaaattcttc tggaagtaca ttgatgattg gatcagtaac
     5101 gggatcgaat aaccagtcat cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg
     5161 atcaaatcct atcatcatga aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa
     5221 cggacgaatc aaccgccaaa gccgatcatt cttttgcaaa tatttgataa acaaaatact
     5281 cgggatcaaa gtgatgaaaa acacagcata atccactaaa aacaaaattt tcacttcata
     5341 aaagtgatgg gctccgctag cagaaactgg gaaatctggc aagctgagga tggttttgaa
     5401 gggattattg agataattca ttaggtgatc aaaatttttc agtaacgtct cttgatctaa
     5461 agaagtataa tccaaaattt gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt
     5521 gatcgtcaca ccaactgcca gactgatcag tgtcagaaat aggctgaaca atccagcata
     5581 ttccatccac tgccatctcg aatttttcat attagaagct ccattcatcc agtgattgtt
     5641 tcacataggt agctgggata ggcaacccag gtacatcttc ttcttttgta aaacctgata
     5701 aaacgagcaa agtatctata ttattgcgta tcccagcttg gatatctgtt tcatagttat
     5761 ctccaaccat gattacttct tctttttgca gccctaatac ttcaacggct ttatccataa
     5821 tgatggcttc tggttttccg atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag
     5881 agatgactga ccctgctcca ggaagaaggc cgcgttcagt aggtatattc ttatctgggt
     5941 tcgttccaat gaaagtagca cctttttgaa tagcaagtgt tgctttaact actttttcat
     6001 aggtcagata attatctaag cctaccacta catagtcagg agcctcttct tcccatgtaa
     6061 atccggcagc taaaatcaag tcaatcaatc ctgcttctcc gataacatag acttttttcc
     6121 cttttccatc tgctttcata aaatcgattg ttgccaaagt agctgtgtag actgtctctg
     6181 gagctacatg aatgtcaaac tcatctgcta gacgattagc tactgtttct ggtgacttag
     6241 tcgtgttatt ggtaacaaac aagaaaggta attttctttt ttgcaattct tccacaaatc
     6301 gtttaccggc cggtatgggt tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat
     6361 atcctttata gtccattcat ccactactct tccctttttc tgatcgtaaa tgctgttttt
     6421 gttttttcta cttgtttagg ttgttcttgt ttttttactt tttcagaggt ttttgctgac
     6481 tctgtttttc gatttttaat aaccggtttt ttgcgattag atgcgactgg tccttttttc
     6541 tcatcgatat acgcacgatt atggtttttt ttcttttttc ggcgctgagt tgtcttttct
     6601 tttttaccgc cgattctctc gatgacaaaa tacgcacaac caaaattgca atattcgtaa
     6661 agatagtctt ccaaggaatc gattcgctga tctggatgcg cccgtcgatt atccgcttgg
     6721 aaaaatcctt ttaggcgtag ttgttcataa ccccaatctc ctacgatata atcatatcgg
     6781 gctagtacat cactgtaacg ttctccaagc ttttctgcat taaatccttc acgataatcg
     6841 gacacaagcc gataatgtct ttcacctatc aaaaaattac tgtcatctaa cagtgtcact
     6901 agctcgcctt tgactttttc tgctttaggc tcctcaacga tttcttccaa agcagctgtc
     6961 agttcttcgg tgactgttgt ttcctgttca gctgatgatt ttttcttagc aatttctgtt
     7021 ttatgctcaa cgttctgctg ttcattttgt tttgcagaca tgcgttcacc acctatcttt
     7081 tttattatac cttgttttta tttgattggg tagtgagctt tcaagtaatc tcctaccaca
     7141 cttcggataa attcagggaa caaaaattca ttttttccag caatctcaat cgttggaaaa
     7201 aatggaacaa acatcaaatg atctacggta gtaaaagtat aatttttatt cagatcgatt
     7261 ggcttgttct tccaaaagac ttgatggtta atagaatcat atgtgattcc attatagacc
     7321 atctgcccaa aaatcttacc gcgaaatccc attcccttca taggatagtt acgtaaaaag
     7381 ttacgatttt tttctatttc taaaactaag cggatcagat catttccacc caatgtgaca
     7441 tttaacaagt gcatcggatg cggcaacgca gtgtgaagct gatcctgatt gacccgccct
     7501 tttggaattt cagttaaaaa cagaccactg tttaatattg ctgcttccgt ctttcctcgt
     7561 tctttcaccg cctcaaaggc tacttggata aacgaatgct cattgaagat atctagactc
     7621 agagaaaatg gcaaatctgc tacttctttt tcttttaata attcatgccc acgttctaga
     7681 taactattta tttcttgttc atcttctgta aaagctgtca ttgtttcggt agggatggct
     7741 ttggctgaat gcttaaggat ttttttctct tcatcgatgg ttaaatggac ttcaccgaca
     7801 tactgcccgt actttccagc ggctgctatt tggacatcat tgattacgtg accttctctg
     7861 aataaatgat gggtgtgcga tcctaaaata acatcaattg ctggtatttc ttttgcaatt
     7921 tggaaatcat cctgaatccc gagatgactc atcaaaacta aaacatccac ttgtgaacgt
     7981 aaatcttcga ctaattctgg caaaatatca taaggattcc gtatgtccca gccattcgga
     8041 ttataagtaa gcggaaatgg agcagtaaat gcaattagtc cgattttcgt ctggtgtttc
     8101 gttgtaatga ttttatatgg tttcgtccat gccggtggct ggagcgtatt tttatcgaat
     8161 aagttatcca ggatgatatc aaactcagca tgatcgtata aatgattcaa ctcttctttg
     8221 gaatttccta cgccttcatt gtttcctatc gtcgctgcat cataaccaac ttcattcatc
     8281 agcaagatat tttcctgtcc attcgttgct tctgacaatg gatgccaacg atccacaaaa
     8341 tcacctagat ctactgcaat cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt
     8401 tgatccaaaa accttctgat ccttggccaa ttttctaaat gagaatgcag atcattcgta
     8461 tgtaaaatca ctattttttc catgtaccct ctcgcctctc tttatcttta tcataccata
     8521 ggcagctttt tcttccattg aaacaagctc tatttcagag tttttcctgc cttgaagcaa
     8581 attgtataag tacaatattc gataaatcag tctggctaaa aaatcaggaa cattcatttt
     8641 tacagtacta taaaaataaa aaaaacagac ccgaaagtct gttgtcgttt cattataatc
     8701 agacacatgg cggaacctgc ttagtgctgc ttccttccag acctgacacg cttcacaagc
     8761 ccaccattgt cctagccttt cggcaaagaa tagtatacct tatttttcat tcgttgacca
     8821 gtcttttttt aatttctttt ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat
     8881 cccacattcg tcttctaaaa tgccaccttc tacatatgct tggtggttaa accgctcgtc
     8941 ctccaaaaga ttcatcaagg taccggctgt tccgcctttt ggatcatacg cgccgaaata
     9001 aacttctgct acacgagaca aaatcatcgc gccgctgcac ataggacaag gctctaaggt
     9061 aacaaaaagt gcagcatctt ccaagcgcca gcttcccact ttttcacagg cttccttgat
     9121 tgcaagcatt tctgcatgtg tagtagcatc ttgcgtggtt tctctaaggt tatatcctct
     9181 tccaatcact tttccatcca atacaaccac tgctccaatc ggaacttcat gaagcatttc
     9241 tgctttcttt gcttctgcca atgcaaggcg catccatttt tctttttcta ctgcatcaag
     9301 atttgtattt accaattacc cttccctgcc tttgtttttt cttgttttac tttagcatat
     9361 gataaaaagg ctgtaactaa cttttttttc tatggtaaaa taaatacaag aggtgaacaa
     9421 gatgaaaatg gataaattac ttgctcttta cccacaagca cagaaaaaaa actctccagc
     9481 atcagaagcg gatatccttt cttttgcaat tgatgatgct tttatctgga tcaaacaaga
     9541 ttcattatct caacaagaaa ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca
     9601 aaaaaaacac ccatggtatc gctatctttt taaagatgct tcttgtgttc aagaaaaaag
     9661 ctttcgtttg atccagcttc acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca
     9721 agatactatt ttggaattat ttcgctattc tgctgatttc tttttttata cagacacaga
     9781 tgcgctctta gtcgaacaaa taaaccgtga ttatcttgat gctactgaaa tcaacggtat
     9841 ttttctatct ttagatgctg attttgatac gacaacgtct gtattcgttg gttctttcca
     9901 ttcacctggc accgacctcg tccctttgtt tgctgaagaa agaaaaattt ttttaacaga
     9961 gcaaaacaat ctttatactc acagccgtac tttttctatg caagatgtgg ctttacatta
    10021 ctatacaaaa gatactgtaa aagatagtaa gttgatcaaa agttacatag aactgatcaa
    10081 acaaaatgaa atggaagtga tcattcaaga attatggaat gaattaggca atgtcagctc
    10141 tgctgcaaaa acactgtttc ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca
    10201 agaacagtct ggtttcaatc taaaaaaagc caacgattta ctgttttgct atcttttatt
    10261 gcttcatgaa cataattaaa gatagattaa aggggttgtg acaataattt tgtcacaacc
    10321 ccttatttcg aatgaatagt gttcaaaagc tagtttctct tacatcattt tttcaacaaa
    10381 atgcattagc cggtctttta catcttcggt tgaatctttg attgaatcac cataaacaga
    10441 gattttcttt tttcctgatt tgatttttgc taacattccc atcaaagaat ccaagtcact
    10501 atctgaaaga ccatctacga tttctagtaa tttttcattt ccatcaaatt tgtcatagac
    10561 gaattttttt actttcgtcc gagtagtcat atgatggatc ttttccatta cttttcccga
    10621 tacaacgact gctgtcgcta cactggcagc ggctgctacg cttaatccga ttgttacttt
    10681 tgttgatgtt ttcataagta tcagctcctt tttatctatc tccatcatag catagacaaa
    10741 aaaataaacg aactaatcca ctcagtgaaa tctcaatcct ttagggaaaa agttctttac
    10801 tgttcctttt acttgtttcc caaatccgac aggtatctta tctaatacta gcaaaaccca
    10861 tccttgattc ccatcacgtt gaaatgtttc tcctgctgtg taagattgcc attccttttg
    10921 agtgatcggc aagcaaggta tattctctat cttttttgta gcaagcgcta atgcatagct
    10981 aggttcaaat cgattctttt tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa
    11041 accatcgaga gaaggggcta actcaggaac ttcccaaaga tggtcgttga acactaaaag
    11101 tcttccagta gcttcataat gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc
    11161 ttttgtcatt tgcactttac ttttttttcg tgtcttcttt tctttatgca tctgattttg
    11221 tccatgaaaa gtcagctttg caacaaaatg tccttcaccc tgatctttat gaggccatat
    11281 acgtattgtt ttttccaaac cagctacgct tccccattca gaacgtcctg aactaacgga
    11341 ttgagtcaaa ggaatctcct caatcgttac tggataattt tctacaagcc agctaatgat
    11401 ctcttcattt tcttctggtg caaacgtaca agtcgaataa atcagttgtc ctttattttt
    11461 tagcattttt attgctgaag acaaaatctc ttgctgacgt ttttgacagt atagcggaga
    11521 ttcttctgtc cactctttta ttgcatttgg atcttttcgg aacattcctt ctcctgaaca
    11581 aggagcatca accacgatcc gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg
    11641 tgcatgattt gtcactattg cgttcgaaac tccccatcgt tctatatttt ctgataaaat
    11701 cttcgcacgt ttcggaaaga tctcattcgt cactaataac cctttcccct tcatttgagc
    11761 agctaattgg gttgatttac caccgggagc cgcacacaaa tccaaaactt tttcaccggg
    11821 ttttgctgca gcagctgttc caacgatcat tgcacttggt tcttggctat attcataccc
    11881 agcttgatgg aggaaagact ttccattgac agtgccaaga aatccctcat tgctgtaggg
    11941 agcgggttgt aattcttcgc tatggtaagt ttggaccatg tccaatcctg ccggttttag
    12001 tggattccac cgaaatcctt ttttcacgct tccttgttca agcgcagaaa aaaaatcgga
    12061 tgcttcttcg cctaaaagaa gacgatattt tttgataaat tgctgtggta acgttgtcgc
    12121 ttcctccttc attccaatgt ttcgttagac attttacaat cttttttacg aaaaataaag
    12181 gtggctgatc tactttttca tgttgctgcc taaggtgact gttcctttaa gatattttcc
    12241 aagtattcat ccgtcacctc gtttttatcc caaacagcgt tcatcatttc aagaaaatct
    12301 tgagaagaat catctgagcg caaacttccc aatgcattaa gccataccaa ttcaagcgac
    12361 ttttcttctt tgatcttcgt gtgacctttg acatatgaga aaaatggccg cttaaatagc
    12421 gaatccatgt ctaaatcaga ttttgcaatg atttcacgaa tactttcatg gatagaaaaa
    12481 aaatctttca ataagtagac aatgcttaaa acatcatcgg aactgcattc agctaagtag
    12541 ttttcaaaat aaacagtgat ttcttctaat atcttctctt ttaacccgct gattgatttg
    12601 aaatgtgtat aaatcgggta tgtagaacaa ccgcagaact ctgctaactc tcttgcagta
    12661 attgacttca ttccatattc ttccacgtat accatacttt tgaacagcaa atattcttta
    12721 ctataatttg ttactctccc catcattctt acctccgtct cttttcatct acccataaat
    12781 aaataacata aataattcta aaaatattat tgataattat caaaacatat ttagttatat
    12841 ccattaaaaa tcaatttttc cgccttaaaa acaagaatat cttgacaaaa taccctagga
    12901 aataaacaat agaataaaca aggtaacaaa aaaattaaat aacgttatat aaaaaagaag
    12961 gcgacactct gtgagaataa cacagactga aatgactttc cactcacagt ttttgtctaa
    13021 ctatatacaa aaacagaata aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga
    13081 gttacttgtt ctacaaatgg tcattcaaaa aaataattgt tctttacaag aattatccaa
    13141 tgacttacat attccaaaac ggacaatcaa agaactgatc cgaaaaataa atgtatcact
    13201 caagcaattt ttattgattg atgaatttat ttattctaat cataaaggag agatccaaac
    13261 caaagaaacc gatcaaacta aaaagttagc tgttttttct aagttaaaat tatgctacct
    13321 aaaagaatcc aatcgtttta actatttgat gttactaatc aattatccac aaactgctgt
    13381 accaaaaaaa tatttacttg aacaacttta tatatcgccc tcctatctcg aaaaactgac
    13441 aaaacagctc aatcattcct taaaaaaatt ccatttacgg atcctatctg taaaaaattg
    13501 ctgtactctt gaaggaaatg agttatctat ccgtctctat ctttactttt ttctttcaga
    13561 tacatttgga ggtaacgatt ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa
    13621 cagtacagat gagcaaatag aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt
    13681 aactttattc atattacgaa acaaacaaca ggcatatttt ccaaaaactt cttctgaaat
    13741 cactgaattg atggacttat tgcagcatac acatgattat tcagaatact ttacttcttt
    13801 ttcagtgagt cgcccttgtg aagagacaga aacagagaga gcctacttta attatttttt
    13861 ctattgtttt tttccaaatc aaatctcttt tcgacaaaaa caggagattg gttttttatt
    13921 ttccacaagc aaaaacctta tttgtcgaca agtcggtcaa ttgatgactt acttaactca
    13981 acagtttcct ctcaaccatt tagaaaataa aataccaatt tatttttatt ttcttgtgtt
    14041 ggcagttgtt tttttcgaac tgttgggtga atccagcagt agtttttcta cactttgctt
    14101 tccagaagcc acgtccaatc attctagttc ttttatacag gaaaagaaat taagagaaaa
    14161 aatcgataca attttttcaa aaaatgggga aagaaaccaa atcagtcaat accttagtca
    14221 attgatccaa cacttactac aaacagaaac gaaagagcag ttactcatcc atttgcagat
    14281 aaccaaacag tttacaggaa attatcatct tcaaagccat ttgcagcagt tatttcgcaa
    14341 agatgctgtt aaaattaccg catattcaga acatgctgat ttgatcatta cagatcagca
    14401 tgagaaaatt tcagatgaaa cggagacttt ctatctggat acaacgcaca atacagtgtt
    14461 atttggcaag ttaataaaag cggttcatga actgtatatg aaaaaaggtt atgagaacaa
    14521 aacaaagagg atgtgacaaa attcttgtca catcctctta tt
//
LOCUS       DANJLF010000062        14312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000062 DANJLF010000000
VERSION     DANJLF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..888
                     /locus_tag="PWT25_002093"
     CDS             <1..888
                     /locus_tag="PWT25_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL1022170.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(928..1620)
                     /locus_tag="PWT25_002094"
     CDS             complement(928..1620)
                     /locus_tag="PWT25_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1022171.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(2057..2626)
                     /locus_tag="PWT25_002095"
     CDS             complement(2057..2626)
                     /locus_tag="PWT25_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1022172.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2700..3023)
                     /locus_tag="PWT25_002096"
     CDS             complement(2700..3023)
                     /locus_tag="PWT25_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022173.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3177..3989)
                     /locus_tag="PWT25_002097"
     CDS             complement(3177..3989)
                     /locus_tag="PWT25_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022174.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4343..5191
                     /locus_tag="PWT25_002098"
     CDS             4343..5191
                     /locus_tag="PWT25_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL1022175.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5336..6277
                     /locus_tag="PWT25_002099"
     CDS             5336..6277
                     /locus_tag="PWT25_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL1022176.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6267..8438
                     /locus_tag="PWT25_002100"
                     /pseudo
     CDS             6267..8438
                     /locus_tag="PWT25_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8550..10721)
                     /gene="copB"
                     /locus_tag="PWT25_002101"
     CDS             complement(8550..10721)
                     /gene="copB"
                     /locus_tag="PWT25_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL1022177.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10741..12927)
                     /locus_tag="PWT25_002102"
     CDS             complement(10741..12927)
                     /locus_tag="PWT25_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL1022178.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12927..13136)
                     /gene="copZ"
                     /locus_tag="PWT25_002103"
     CDS             complement(12927..13136)
                     /gene="copZ"
                     /locus_tag="PWT25_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL1022179.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13149..13589)
                     /locus_tag="PWT25_002104"
     CDS             complement(13149..13589)
                     /locus_tag="PWT25_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL1022180.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13663..14202)
                     /locus_tag="PWT25_002105"
     CDS             complement(13663..14202)
                     /locus_tag="PWT25_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1022181.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4157 a         2927 c         2530 g         4698 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt
      961 gtcatgaaaa tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat
     1021 catgacgtat actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac
     1081 atttcctaaa gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac
     1141 tctagctagt tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt
     1201 agcaatgtgc catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat
     1261 gtgggcgtaa tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc
     1321 aattactggt ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc
     1381 ataggctagt ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg
     1441 atcgccccaa atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg
     1501 agtgtctaat gttaatggaa taccatattt cattgctgaa tctcttgcca attcaaagta
     1561 tactttatag ttcttttcaa aagtttcttt atcatgtgta tgttgtaact cgatttgcac
     1621 aggacttttg gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg
     1681 aaaacaccaa cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta
     1741 gaagatatca aaggtttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat
     1801 atttctcccg aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa
     1861 ataaaaaagc actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa
     1921 ttttatttat aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt
     1981 atcttaaagg cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag
     2041 tgcgaacatg gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca
     2101 tcaattgaga ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg
     2161 gttttcttct taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca
     2221 cgactccgcc aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta
     2281 gattttttgc tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct
     2341 cttccatccc ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta
     2401 tttcttttgg ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca
     2461 agagcgtttc gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata
     2521 tatatacgcc agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca
     2581 cttctgtgat cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt
     2641 tactaatcgc tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt
     2701 tatcggtttt tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa
     2761 ggatggtaca gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcgatg
     2821 atcactagat ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt
     2881 tttcgatgtc ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca
     2941 tagcgaaaaa aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc
     3001 tggagtgttt tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg
     3061 atcgctagcc gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc
     3121 tgataataac gaacttcttc tatcagccgg tcatggatac caattacgta acgaagtcat
     3181 tcttggtaaa gtacttttgt cagctttgat ttgctttcgc tctcaaattc gctattcagc
     3241 caaaacagcg aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct
     3301 tctatccgtt tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga
     3361 taattttttt cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat
     3421 tcaaagaata gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc
     3481 ttcaataatt ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc
     3541 ggaaaatctt tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc
     3601 tgtgcttttt tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct
     3661 tgcgtagaac gatcgtactt ctgctcgtaa ggttcattca atagcagatt tttcagtaaa
     3721 caaaacgggg gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga
     3781 tcataaacta actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc
     3841 gccaaacgtt tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa
     3901 tcagcttttc gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca
     3961 ttttttgata atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat
     4021 tgtttgatca aaatgggttc actcctaaaa agataaaaaa ctccctttct atttatttaa
     4081 atacgcaaat cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa
     4141 aacgaatgta ctttaaaaaa acataataag atcactttgc ttgtatgatg tattcccttc
     4201 cattacaatg aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat
     4261 agaagaatcc aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcag
     4321 caaacgttca actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt
     4381 cgaagatatt cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca
     4441 gggaaccttc agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt
     4501 ttccgtatac aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac
     4561 gatcttgtta gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga
     4621 agcaatggcc gagaatcatg caacgatcga caacaataca taccctctag acaatcattt
     4681 ttttgtctta gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc
     4741 acagctcgat cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact
     4801 tttgctactt ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag
     4861 ttatgaaata gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc
     4921 atcttatgcc ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat
     4981 cagtccacga ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg
     5041 gagaaattac gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg
     5101 cctgattttt tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct
     5161 tgttgcacaa attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag
     5221 attatttcat taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg
     5281 tattcaacaa ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga
     5341 gcttgatttc attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac
     5401 tcgtttatcg caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt
     5461 ctttacctct ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga
     5521 gattttgatt cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag
     5581 aggatgccat gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag
     5641 aaaaagtgca aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat
     5701 tcaagcagaa aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag
     5761 aggtcttgat tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga
     5821 ttggaaattg tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga
     5881 agaatgttct cctctttttt gcttttgggg tttgcccagt ccaaaatttg aacctctgct
     5941 agacttgtat ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt
     6001 aggcaaccgc gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc
     6061 tggcgatgag aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct
     6121 ctttgttcct gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg
     6181 aacggtttat cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat
     6241 atgttcattg ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg
     6301 tttactcctt tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc
     6361 aaaacagcta ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc
     6421 ctaaaaaatg ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat
     6481 attttccttc gaagacattt tccatattat ggatcttaca gtttttttcg tcagtcacag
     6541 aggaattctt ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt
     6601 ttctcataat gttgctgatc tatttattta ttacattgac cgtggattac gactattggt
     6661 acgcaccctt tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag
     6721 atttactgta cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc
     6781 aatttttctc catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc
     6841 tgcttatttg tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc
     6901 aatctctcga ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg
     6961 acacactcca tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg
     7021 aacagcttgg tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag
     7081 gaaatggcta ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag
     7141 atcaggacgc acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa
     7201 ccgttataga agacaaagaa aacattacat tgaccctaaa aaaacaagtt ttcttacctt
     7261 atccttatgg aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact
     7321 atttgcctga aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt
     7381 tacaaacaga aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg
     7441 atgatttaga aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg
     7501 aggacttggc ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg
     7561 tctaacagta tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata
     7621 ctccaaaaga acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg
     7681 acaacttttc aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga
     7741 caaaaggttt tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca
     7801 gcattctcaa tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc
     7861 ttccatttga accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa
     7921 caaccgaaac cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa
     7981 ctatttccag tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt
     8041 ataaaatcgt cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt
     8101 tattcagaaa atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta
     8161 ctaatacgat tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac
     8221 ccgcagaatc tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc
     8281 gtttcattcg attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg
     8341 taaaattcga taaaaaaacc tatttagaaa taacgcatat aataagaaaa atggtataca
     8401 aaaaaattct gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta
     8461 cttctggaat gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac
     8521 acgttttttg tcacaacctt ttttcattat tatctgattt tcaatgtcaa agcatttaga
     8581 gctacaacta ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata
     8641 ccaattggcg caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc
     8701 caccagaggt tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa
     8761 atatcttttg gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc
     8821 cctgcaccga ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca
     8881 tcacccacca tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta
     8941 tcatctggta ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg
     9001 gcagcgtttt tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt
     9061 cggataaatt cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga
     9121 tgattttcta aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat
     9181 gcttgttcta cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt
     9241 tgttttttga ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga
     9301 taaggcttga tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg
     9361 gcattcgctt ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca
     9421 agtacttcga cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc
     9481 atgatcacat ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg
     9541 tttttagctg ctattgatgt ggaacgagca acaactaatg ggatagctaa acctagagca
     9601 tgaggacagg cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc
     9661 aagaacaacc aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat
     9721 tttgccactt tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc
     9781 atttccataa ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc
     9841 gttccatccc cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct
     9901 cttgattcgc cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca
     9961 tctgttggca ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc
    10021 aatgaaactg tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat
    10081 tctgctagtt tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga
    10141 cctagtagca taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta
    10201 tgtggattga gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc
    10261 cccatcgcga tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca
    10321 cttaaaaatg gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa
    10381 tcagaaccag gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga
    10441 atgattggga tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc
    10501 atggaatgat ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg
    10561 ctgtttgcta tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg
    10621 tcatgtttca tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct
    10681 tttttatgct catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc
    10741 ttactttttc atgtgtcgat ttaagcacag ggagttcaac aatacactga cggaactaaa
    10801 cgccatcgct cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc
    10861 aatcgtatta tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct
    10921 agacaactcg attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc
    10981 tgtttccatc gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa
    11041 ggccggtgca tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag
    11101 tttttctacg taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc
    11161 aataccaact tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa
    11221 aacatctaat cctttattct ggagctgctt gattgcttgt ttgacttcta gtttgatttg
    11281 atcagccacg gcaatcaacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt
    11341 tccctgctgt tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc
    11401 aattaatcgt ttacgtgtac ccgcaaaata gcgtactccg ttaatcgtac cgctgattcc
    11461 agcacctggg tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc
    11521 gtattccacg attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat
    11581 agataggact tctttagaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt
    11641 tccagtttta tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc
    11701 tttaattaaa attccatttc gtgcaccaac acccgttcct accataatag ctgttggagt
    11761 cgctaaacct aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc
    11821 gagttgccaa tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa
    11881 tacgatcgga acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct
    11941 cccttgagca tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg
    12001 tgtaacttcc atgtgaagta gaccattcgt attgattgtc ccgccaaaga gcgtgtcatc
    12061 cggattcttt tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct
    12121 tcctgaaata attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc
    12181 accaacgact acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc
    12241 ggtttttgtt tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt
    12301 atgttccaaa tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata
    12361 aagatcttgg gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga
    12421 tgttccaatt gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc
    12481 acctttataa aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac
    12541 gattggaaaa tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat
    12601 tgacaacatc aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt
    12661 caaatacgct tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc
    12721 accatagccg atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt
    12781 gacacttgct ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt
    12841 cagctctttt tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt
    12901 atccattttg gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg
    12961 attgcttgac aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt
    13021 gcttttttca aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct
    13081 actcttgctg cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt
    13141 tcttcctcct attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc
    13201 gtatctactg gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc
    13261 aaagtggctt cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg
    13321 gaaaaaagat tttccgtcgc actgcgaact gtttccatct ccgagacagc tggataataa
    13381 atgaattttt ttccttcttg ttctgtccag atcacttctt tttttaccaa acgacccagc
    13441 aaagttttga tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct
    13501 tgggctgtcg tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg
    13561 atctttactg gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat
    13621 cattatctta tgaatacaga ttacacttgt aaacaaaaac tgtcaattat ttttgctctc
    13681 tattttcaat ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt
    13741 atttcttgca ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa
    13801 atgccagata aaagataaaa attctaagta ttccctgttt agtttctccg aaaatgattc
    13861 aggcatatct ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg
    13921 gtagatggcg agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct
    13981 ttcctctaat gtagaagtat aatttccttc aacgatccat gacggattag ataccataaa
    14041 caattgctgt tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca
    14101 taaaaggtct agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt
    14161 cgattttccc gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc
    14221 tttggaattt ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca
    14281 aaagtcgtgt caaagtccct tgtttcgaat aa
//
LOCUS       DANJLF010000063        14183 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000063 DANJLF010000000
VERSION     DANJLF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            164..1309
                     /locus_tag="PWT25_002106"
     CDS             164..1309
                     /locus_tag="PWT25_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL1022182.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="PWT25_002107"
     CDS             1446..1790
                     /locus_tag="PWT25_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL1022183.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="PWT25_002108"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="PWT25_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL1022184.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="PWT25_002109"
     CDS             complement(3485..4084)
                     /locus_tag="PWT25_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL1022185.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="PWT25_002110"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="PWT25_002110"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL1022186.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="PWT25_002111"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="PWT25_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL1022187.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="PWT25_002112"
     CDS             7290..8408
                     /locus_tag="PWT25_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL1022188.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="PWT25_002113"
     CDS             complement(8468..9760)
                     /locus_tag="PWT25_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL1022189.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9892..10470
                     /locus_tag="PWT25_002114"
     CDS             9892..10470
                     /locus_tag="PWT25_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1022190.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="PWT25_002115"
     CDS             10599..10976
                     /locus_tag="PWT25_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL1022191.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="PWT25_002116"
     CDS             10909..12981
                     /locus_tag="PWT25_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL1022192.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="PWT25_002117"
     CDS             complement(13192..14139)
                     /locus_tag="PWT25_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1022193.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         4605 a         2576 c         3020 g         3982 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttc
//
LOCUS       DANJLF010000064        13810 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000064 DANJLF010000000
VERSION     DANJLF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(221..1423)
                     /locus_tag="PWT25_002118"
     CDS             complement(221..1423)
                     /locus_tag="PWT25_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1022194.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="PWT25_002119"
     CDS             complement(1426..1620)
                     /locus_tag="PWT25_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL1022195.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="PWT25_002120"
     CDS             complement(1667..2515)
                     /locus_tag="PWT25_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDL1022196.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="PWT25_002121"
     CDS             complement(2720..3070)
                     /locus_tag="PWT25_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022197.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="PWT25_002122"
     CDS             3665..4111
                     /locus_tag="PWT25_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL1022198.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="PWT25_002123"
     CDS             4735..5727
                     /locus_tag="PWT25_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1022199.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="PWT25_002124"
     CDS             5743..6627
                     /locus_tag="PWT25_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1022200.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="PWT25_002125"
     CDS             6676..8283
                     /locus_tag="PWT25_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1022201.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="PWT25_002126"
     CDS             8284..9768
                     /locus_tag="PWT25_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL1022202.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="PWT25_002127"
     CDS             9945..10937
                     /locus_tag="PWT25_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1022203.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="PWT25_002128"
     CDS             10989..11858
                     /locus_tag="PWT25_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1022204.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="PWT25_002129"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="PWT25_002129"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL1022205.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DANJLF010000065        13731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000065 DANJLF010000000
VERSION     DANJLF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            1..1452
                     /locus_tag="PWT25_002130"
     CDS             1..1452
                     /locus_tag="PWT25_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL1022206.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="PWT25_002131"
     CDS             1801..2433
                     /locus_tag="PWT25_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL1022207.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="PWT25_002132"
     CDS             3021..4460
                     /locus_tag="PWT25_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1022208.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="PWT25_002133"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="PWT25_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="PWT25_002134"
     CDS             5222..5437
                     /locus_tag="PWT25_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022209.1"
                     /translation="MNAGIADMNLIKKNLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="PWT25_002135"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="PWT25_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="PWT25_002136"
     CDS             complement(6097..7008)
                     /locus_tag="PWT25_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1022210.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="PWT25_002137"
     CDS             complement(7027..8031)
                     /locus_tag="PWT25_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL1022211.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="PWT25_002138"
     CDS             complement(8028..10151)
                     /locus_tag="PWT25_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1022212.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="PWT25_002139"
     CDS             complement(10156..12603)
                     /locus_tag="PWT25_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1022213.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="PWT25_002140"
     CDS             complement(12590..12979)
                     /locus_tag="PWT25_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1022214.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="PWT25_002141"
     CDS             complement(13039..13542)
                     /locus_tag="PWT25_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL1022215.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13716)
                     /locus_tag="PWT25_002142"
     CDS             complement(13555..13716)
                     /locus_tag="PWT25_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022216.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4222 a         2709 c         2509 g         4291 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa aaccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DANJLF010000066        13386 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000066 DANJLF010000000
VERSION     DANJLF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            6..305
                     /locus_tag="PWT25_002143"
     CDS             6..305
                     /locus_tag="PWT25_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022217.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(402..836)
                     /locus_tag="PWT25_002144"
     CDS             complement(402..836)
                     /locus_tag="PWT25_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1022218.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1068..1778)
                     /locus_tag="PWT25_002145"
     CDS             complement(1068..1778)
                     /locus_tag="PWT25_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL1022219.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1768..2628)
                     /locus_tag="PWT25_002146"
     CDS             complement(1768..2628)
                     /locus_tag="PWT25_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL1022220.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2633..3271)
                     /locus_tag="PWT25_002147"
     CDS             complement(2633..3271)
                     /locus_tag="PWT25_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL1022221.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3286..3585)
                     /locus_tag="PWT25_002148"
     CDS             complement(3286..3585)
                     /locus_tag="PWT25_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1022222.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3628..5112)
                     /locus_tag="PWT25_002149"
     CDS             complement(3628..5112)
                     /locus_tag="PWT25_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL1022223.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5132..5593)
                     /locus_tag="PWT25_002150"
     CDS             complement(5132..5593)
                     /locus_tag="PWT25_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1022224.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5611..6678)
                     /gene="ulaG"
                     /locus_tag="PWT25_002151"
     CDS             complement(5611..6678)
                     /gene="ulaG"
                     /locus_tag="PWT25_002151"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL1022225.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6896..7666
                     /locus_tag="PWT25_002152"
     CDS             6896..7666
                     /locus_tag="PWT25_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1022226.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7939..8766)
                     /locus_tag="PWT25_002153"
     CDS             complement(7939..8766)
                     /locus_tag="PWT25_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1022227.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9334..10569
                     /locus_tag="PWT25_002154"
     CDS             9334..10569
                     /locus_tag="PWT25_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL1022228.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11568..11828)
                     /locus_tag="PWT25_002155"
     CDS             complement(11568..11828)
                     /locus_tag="PWT25_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL1022229.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11855..13156)
                     /locus_tag="PWT25_002156"
     CDS             complement(11855..13156)
                     /locus_tag="PWT25_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1022230.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4269 a         2775 c         2093 g         4249 t
ORIGIN      
        1 aaaacatgga ttatactgtt gaatttgaca gtgaactact taagcaattt ccacaagaag
       61 aacatttcat gtataaagaa gcaatttccc acaaagtgac agagtatttg gagcataatt
      121 tttttcgtat aaagccagtg cgtactcgtt cggcaaaagg aatatacgag atgaaagtca
      181 aaattaataa aaaaaattat cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt
      241 tttatattag ttcgaatttg caaaaaattg tatttgataa agaggtgagt aaaaaaataa
      301 aatagctgag cattggagga tagattgagt gatactaagt ctatcctctt gtttttctaa
      361 agaactaagc tagaattttt tcttttgtac gttattatat gttatttgtc attaccaaaa
      421 agcacctttt ttcttctagt atctatgaca taagaaatac cacccatttc ctttacttga
      481 acaaatccta ctgattcata aaattttttt gttttctcag tatcatctgt caataattcg
      541 atttggactg gtccataact agtgatcttc tccaaagctt cattcaaaag cactttgcct
      601 attccttggc gctgatacct cggatgaatg aggatatctt gaataaatgc gatcgtcgca
      661 aaatcagtga tccaacgaac gattccgata atcttccctt ccccattacg ctttatcaga
      721 gattcagatt gttcaaatgc tctagctaac cttgcagtgt cttttgtgta attcgtccaa
      781 ccaacgctct tatataattc agaaatctca tcgatcataa gggaatcaat attcattttt
      841 ttcttccttt cttatacaat tctccctcta ataatacagt tctttgacta tttaatctat
      901 cacatataac tctcctgtaa agattctctc gattccttaa aattcctctt tccttttaga
      961 atgcatactt tttgataaat aaaaaggttg tgataaattt tcttcacaac ctctgtttct
     1021 ctctatcaag tgttatcttt taattttcta cccttaccct agcatctcta ttgcccgtag
     1081 tatgcatact ttccatgttt gcgaaaataa tgtttatcta gtaaatattg cgggatttca
     1141 catatattgg ggttgatcag ctcgttttct ttggccatca gacaaatttc atctaagatc
     1201 aaactgtttt ctactgcttt cataggtgaa tccccccatg tgaacggtcc atgtccatag
     1261 acaagtactc cagggacttc gtttggatct aattttcttt ccttaaacgt ctcgacaatc
     1321 acattccctg tatgaacttc gtaagcttct cttacttctt ctttcgttaa ttggcgtgta
     1381 caaggaacct taccataaaa agcatcagca tgagtcgtgc cataagctgg caaatcgcgt
     1441 cctgcttgcg cccacatgac ggagtgcgtg gaatgagtat gagtaatagc agttatatct
     1501 tcaaaagttt gatacaaaac aacatgtgtg gggagatctg aagatggttt taagctatct
     1561 tcttctatta tatttcccga caaatctgtt accaccattt gatctgcttg catgcattca
     1621 tatctgacac cgcttggctt aatgacgatg actcctagct cacggtccac ttcactcaca
     1681 ttgccccaag tcaatttgac taatccgtat cgcggcaatg ccagatttgc ttcaaggaca
     1741 cgttgcttca ttgctgcaat tttttcttca caagacatca tagccagcct ccttcaattt
     1801 agaacttaag tagtcttttg cctgctggat ttctcttcga aagtcttggc ttttttcact
     1861 ccacatttca atcagaaatg ttccatcata atctagacgt ttcaaatgtc ttaataatcc
     1921 aagaaaatca acacaaccgc taccaaaagg aacatcacga aactggcctg aatattcttt
     1981 tgttacaggg taagtatctt ttaagtggat agcagtgata taatcgatcc ctttctcaag
     2041 ttctcgagcc ggattatttt ccggccatgc ggacaaattg cctaggtctg gatatacttg
     2101 tagaaaaggt gaatgaatct gttctttgat ttctaaaaat tttgaaatgg agttcataaa
     2161 tggatcatcc atgatttcca cggaaagaat gatgccatat ttagaagctt cctttacgct
     2221 ttcttttatt ccttcgataa agtcttctct ggaattcatt gatttctctt cataataaac
     2281 atcgtatcca gctatttgga ttactttgac gcttagatta tgcgccaaat ggattgcttt
     2341 tttcattagt tgtttcgctg cttttttctt ttctggatca gcagaaccaa acggaaatcg
     2401 ccgatgcgca cttaaacaaa tcgaatggat acgtacaccg gagctgaaca tctcttctct
     2461 cagctcagct atcttttctt cagaccaatc taatcgtgct aaccgctcat ccgtctcatc
     2521 aatcgacatc tcgataaaat cgaagtccat ttcttttaca agggaaaaac gttctttcca
     2581 tgaaatatct ttgggtaacg ctttttcata aatcccaatt tgagccattt ccttaccccc
     2641 agatacgaat gatctcatct ttgaagtttt gtgcggcctt catcggatca tttgctgctg
     2701 taataccacg accagtgata aaggtataca catcgatccc ttcaaataag tgtaatgtgt
     2761 gtggatttaa tccaccagtt acagaaacat taaagcctag ttcaatcagt tttttgatct
     2821 tcgataaatc tttttcaccc caagtttcac cagataacag cgcatcacgg ctttgatggt
     2881 agattacctg ccggataccg atgttatgcc aatccatggc ttgttcaaaa gtccaattgc
     2941 cgtagagttc tacttgcaat tcacctattt ctttttgtgc tgcctccata gttggaatcg
     3001 ttgcacaaca aatgactgtc atccagtctg ctcctgcttt tgcaacatta ctcgccactg
     3061 ttcctccagc gtctgcacat ttcgtatcag cgactagttt tttatctggg aacatactgc
     3121 gaatacagcg aatcgctttt tgtccttcct gtaagcaaag aattgtgccc acttcaataa
     3181 tatctactac atcaccaact ttcataacat ctcctaatgc atgttctaag tcattgtgat
     3241 ctaacgcaat ttgcaaattc ggcctgctca tcttagttcc ttcctttact tgtttttttg
     3301 gtaccacttt gtttcagcaa atttttcact gatttctttg actgacatca catttttgat
     3361 accgataact gtaactcctt ttttctctgc ctgagtaaac atattcaaaa aattgactgg
     3421 cgtaaaaact acatcgtatt ggccagctga actttttccc tctgaaatgg cacaatgatg
     3481 gattttggta attggtacgt ttagttcttt cattgctttt tccacacttc tcatcatcat
     3541 caagcttgtt cctgatccat ttgcacacga tactaaaatt ctcatcttaa tccctccatt
     3601 atgagtgttg tctaatcaga ttttctttta tactctctgc ttttctgcat atgtgtcata
     3661 gtcttctaca atcaaaaaat aaccatcagg atttttccga tattgtaact gcggaatagc
     3721 caataaacca atgacaacta cggcaattcc agcataacct aggactttca tgattactgt
     3781 aaacaatggc caagctgttg cccagtcgaa catcccgata taaccaccgt atgttgacat
     3841 cccaatccat gtagcgaata atgctgaacc gcctacttgt atgattcctg aaagaaacgg
     3901 gaacaggcaa gcggctttga atccacctcg gttattggca taaactgcta taacggcatt
     3961 atcaaagaac aacggaatga aaccggctat gacgatcgtt ggtgatttca ataaaatcag
     4021 taaaccaatc atcaaaaact gtcccaaagc accaaacaag aacccaatcg ttaccgcatt
     4081 tggcgaacca aagccaaaag tagcagctac gtcaattcct ggaacagctc ctggtaaaat
     4141 cgtgtttgag attccttgga aagattgagt tagttcatcg acgaatgtcc gaacacctaa
     4201 ttgcaagatc gccagatata ccgcaaaatt taaagaagtc gtcataatgt agaataagaa
     4261 actctgccct tcttgcatga attgcgcttg aatcaaataa tctttgccta gtacaatcag
     4321 aatgatccca aagaaaaata gcatgagtaa cgaagtagca accatattct cattgaaaat
     4381 cgacaagaat cctggtaatt tgatatcttc aatttttttg tcttgcttga ttgatctatt
     4441 tttcttttca tcccgctttt tcatccactc agctaatttt gcaaatataa agattccgaa
     4501 catttgctga tgtgccactg caaatccggc accttctgtc aattcctgcg tgattcctac
     4561 tgttaagtta gaagctactg tccaataaca acctaaaatc agtcccataa aaatcaatgc
     4621 ttgtatccta cctagatcag gaaagcaaaa cagtaaaatc caaaaagcgg tagctgcttg
     4681 ttgaacctga acgtttccgg tagtgaaaac ggaacgcagt ttcgtatatt tctgaaaacg
     4741 gactaataag atatttaaaa caaatgcaat cagtaataaa atcatcacat ctccaaaggt
     4801 acggccaaat gtctccatta atccagcatc cacagcattt tgtccaaaat aaggatctgt
     4861 aaccatggca tccaaattga accgttcctt tagtccaacc aagattggcc gaaaattatt
     4921 gaccaaacca ccagagccaa cagtcaaaat aaagtaaccg accgttgcct ttaagaatcc
     4981 agcgatgcat tcatgaagcg ggcgtttcag caaaatatag cctaataaca cgataaaacc
     5041 aatcaggtat gctggctgcg tcaagatatt tgttgcaaaa tacgtccaaa tgcttaacaa
     5101 aaattccccc atcttttttc tcctctatca attaattata ttctgccatc acaacttggt
     5161 aatcatcgat ggagttcgta gagatgagac gctcaaccaa tccatctgtc attaataatt
     5221 cggataattt ttggatattt tctagatgtt cttctggatc ttttgctgcg agtgtaaaaa
     5281 acaaactagc cgttttctcc tcattctccc cttcactttc aaaacggatt ggctgtttca
     5341 acttggtaaa tgaaatagct gttccaaaaa caccatcact tttttctgaa gaatgcggca
     5401 ttgctacttg aggtgcgatc acaatataag gaccgtattt ttctacacaa tggatgatct
     5461 cctccacata cgtttcatca ataatttcct tatcgatcaa tacaagacag ctttcggcga
     5521 tagcttcacg ccaatccgtg atttctttat ctgggtaatg aatcaattga tggtcaaaaa
     5581 agtatttcag caagaaactc cccttctttc ttataaaaac gacggaaacg gtgtatcatt
     5641 ttcaatcgag aagacatctt caaatcctcg ataaaaatta aactcaagat catctttgtt
     5701 tgtcggatag atgaatttcc cgcctacttg ccagatataa ggtttaaatc catacttcag
     5761 gcgatccttt ttgaatttcc aaatttcagt gatctctttt ggatcagcca taaaattaga
     5821 ccaaatatcg tagtggacag gaatcactac ttttgcattc aatgactccg ccatgcgcag
     5881 catatcaaca gaagtaactt tgtctgttat ccctcgtggg ttttcaccat aggcccctaa
     5941 acagacatcg attttgtgtt cattaccatg tttggcaaat aaattggagt aatgtgaatc
     6001 tcctgcatga taaagattcc ctcctgatgt ttcgaataaa tagtttactg caatttgatc
     6061 catctcctga ggcatttttc cttttaatac ctcttctgga tcttcacaag tgaccaacgc
     6121 tgtccgatca aacgcttcta aagctactac attgatgtct ttgattttga ctgcatcacc
     6181 tggctttact gtaatcgttt tttcttcagg aattccccat ttttgccacg tgcgaaccac
     6241 ttcttttggc ccaatgaatg ttgcttcagg acaattttga tgaactgctg cggctgtatg
     6301 gatatctaga tgatcggaat ggatatgtgt aacgattaaa gcgtctacat tttttatcgc
     6361 aaaaggatcg attacgaaag gctgagtacg taaattaggc tgcatatttt gaacgccact
     6421 catacgcatc atctgatggc cttttttcat catgccattc ccatgactac gttttcccgt
     6481 cccacaccat agatcacata aaatattcgt tccttcatga cttttcagcc aaattcccgt
     6541 acagcccagc caccacatag caaaagttcc aggatccact acttctgttt cgatttcttc
     6601 attcaagtat gtgccccact caggaaatgt ttctactaac cacttttctt ttgttacttc
     6661 attgacagtt ttactcattt gcttgctccc cccatatcgt tgttccaact tacaagtaaa
     6721 tattaatgtg tatcagcatc catgaaaacc cttttatatg accgtttgtt ttttatatga
     6781 ttttctttca gtaatcaatg attgtttcag tataataaag acagatatga atgtttaaat
     6841 gaacaactag tcaaataaat ccatggatca agatgtccct caggaggcaa aagtcatgaa
     6901 aaactcactt gtcaatattg aaaaacgtca aaaagaaatt ttggaactgc ttcagcagac
     6961 tgaacaacta tcaactttcg aattagctga atcccttgat gtatctatta gtacgatacg
     7021 aagagattta aatttattag aagaaaaaaa cgagatcata cgcaattacg gttattgttc
     7081 ttataattac aaaaaccaga ctgattttga tcaatctggt cctgaaagga ttaaacaagc
     7141 gattgccaga gaagctagta aatacattaa cgattatgat acgctgttta tcaattctag
     7201 ctcgactgcg ttgaaaacac tggattttct tcaatctaac catttgacag tcattacaaa
     7261 caacgtgaaa atcaattact ctccgcacaa agataattgt agttatattt taactggtgg
     7321 agagatgcgc tttccaaaag aagcactagt gggcgacatc gcattaaata cgatttcttc
     7381 agtcaatgca gacatttgta tcattggctg tagcggagtc gatgtagtac atggggtcac
     7441 aacaaagata ttaaatgaat caacgatcaa cgaaatgatg gtcaacaaga caatcaaatg
     7501 taagatttta gtcgctgatc atcgaaaaat cggactgaca tcaaagttca agattgctga
     7561 tatttcagtc tttgattatt tgatcactga taaattttgt tctgaaaaag tattagagga
     7621 gatcagaaat acggggataa aaaccattca agtagattca ttttaagaac caggacgtaa
     7681 atatgtaaat aggattatca aaaaaatgaa taacaaattt atcgataaat gaaaaacacg
     7741 ctacgctcaa attgataaag gaatcccaag atgtatccat ccaatcgata ccatcataat
     7801 ctattgcttc ttttataacc gttatatgtt gcctaaatag gaatcaaaca atatcgaaaa
     7861 aaggaggtat gacaaaagtc acacctcctt ttttcgaata aacggtattc aaatcagaac
     7921 actgatttca caaccacttt atttcaggtc gttttcaatc acctttaccg cgtaatcatt
     7981 tccgttgaaa cgcgctattt tttttgcgtc catatatctt tctaaataat catcttctcc
     8041 taattgctta tggcaagaag caatttcata aagcaagtat tccaatgcat aagtcgattt
     8101 atgtttctta ttatactcaa tacccttctc acataattgg attgctttat cataatcctc
     8161 gattttacgg taataggtgg cgatatcata atatacaatg cagaccatca gattatcaaa
     8221 atcagtctca ctcaatattt taagtgtacg ctccacataa actttcgcaa aatctaattc
     8281 tccttttgac aagtagatca ttgccacgtt tgcagctgct tgtaagtgat ggatatctgt
     8341 ttcggtcgtt tgttccaaca cttgattaaa ataaaataat gctgcatctg gttttttcgc
     8401 ataaagatga tcaagcagtc cttgataaaa taaaatttga tttaaaagat cagaattact
     8461 tactttttcc tgatcgatcg tttgtaaaat tttataagat tcttggtatt ttgatacaga
     8521 attcaatgat tgaatctcat ttattttttt cctgattttg tcttcttctg tttctactaa
     8581 tatatcggag atctcgacat tcagcttttt gcaaagcgca cttaagatat tgatgcctgg
     8641 cgacatattc ttgttttcga tattgctgac agttgcttgt tcacaaatgc cttctgctaa
     8701 ttgtacttgt gttaacccct ttttctttcg gtattctttt agtaatgctc catctacttt
     8761 aatcaaaaca atttccttcc ctttaaaaaa ttatttctcc tttatatcat acacaaaaaa
     8821 aagttctaaa caaccaaaaa tgtttagaac agaataaaaa ttttaatttt aaaataatat
     8881 tcaaattaaa caactggcgt atctgctcct tgatttcttt ttgtgttatc tgaataatta
     8941 tcgtttacag ttataacaca tagtgaaaat tgtcatggcg agaaagtcta agaatatttt
     9001 taaacataac tatttctttc ttacgtgtgt ataatgacga cagcacacct atcaattaat
     9061 ataattattt tattttttta tgttctcatg tttattttta atgacacaac ttctcgtatt
     9121 tatctggcac aacatataaa tcgatcaaaa aattgttgcg tgtttttttg aaaattggag
     9181 tttcaacttg attacaaaat ttaaatttag aagttgtgaa aacagcgaga gacagtttaa
     9241 tattcaaaat atacataggc tactcgaacg ctgtgttttt tagtatacta gtaagaaacg
     9301 aagggataga ctcattccaa taaggaggat ttcatgaaga caattctttt tgctcctgca
     9361 gtattcaatt tagctgagac aacacgaatg attgaggttg ccaaagctat gaaagaccgt
     9421 tattactgtg tattctttgg cttttctaaa acttttgctc ctttgatcac agaaagtggc
     9481 tttgagtatc atttactagc gccgatcctt acgaaaaaac aagaagaaca aatcataaaa
     9541 tttgaccagt tgcgtacact taaaaaccct tttaccaaag aaatggttgc ctctcgtatc
     9601 actagtgaac agagtctgat tgctaagttt gagcctcgac tgattgtgac aggttcgaat
     9661 gtgacaattt ttttatcggc ccgcaataaa aagattcctt tagtatatgt taaaccctat
     9721 gctttgagtg aacctttttt tcaatcagag catgcgctga tgccccacga gttacgtttc
     9781 ttcggccctt taagcaatcc cctttggcac tggacaaaaa agcgtatagt aaatacccga
     9841 tggatcccca atgccttcaa acacgctgca caaaaagaaa tgcttccttt accaaaaaca
     9901 agtttacaaa tgatggatgc tgatctaaat ctcatcacaa cgcctaaact atttttggaa
     9961 gaagaacagc tgccggaaaa ttatcgaatc gttggtccaa tttttgctaa gctcagtact
    10021 tccttgcctg aagaagtgct aacttttata cagcgaaagc aacaagaaag aaaacgtatt
    10081 atctatttcg caatgggtag ttcaggcaat ccgcgtatgt tacgcaaaat tttgttcttc
    10141 ttacaaaaac aaccggatct agcggttatt gcacctattg actatctgct tgatgacatt
    10201 caatctgact cagaagatct tttaataaca gaatatctgt ctagtcatct tttgcatgaa
    10261 ttcattgatt tttcttttat ccacggcggg gaagggaccg tccaaactgc ctgtgaatcc
    10321 ggcaaaccgt ttatcggtgt ggggatgcaa tttgagcaaa gctgtaatat tgactattgt
    10381 gtaaaatacg gaaacgcaat cgccctcaca aaaccagtga ctgaaagaaa actgcagatt
    10441 gctctgcgcg aagtttcttc acccaagatt cgtcagcagg cacgcacgct gcaagccgtt
    10501 tttccaataa atggtccaca acaagcaatt caagaaatcg aaaccttcct gagcaaccac
    10561 ccaatatagt ttcaagtaga aaaagctttt ttaatagaga ctcatcagta atttcgctat
    10621 tcgagaccca gacagtgaaa atacaaaaaa agtaaatagt ggattgaaaa gttagagtta
    10681 cttaaattac ggtgtctaaa aaacacaaaa tcatccttaa aataaaagat ctgacgctat
    10741 ccaaatgagc ataggtagcg tctgccattt attcttagtc aaattctttg acacctttct
    10801 ctggttggat catctcaacg tttcttcaaa agaaagacca taataattag gcaaatacat
    10861 tttcttaaaa tgtgtcatat agatctagaa gcatcgcttt gtagagattt gtgccgctag
    10921 tcccctcaac aattactaaa tcaaatgtag gatttaccac tttatctttg ataatcaacc
    10981 tccctcttct gttgagacaa tgatctcgca atccgatgca tactcctata taaactgcca
    11041 gcaatcccca catattgagt agatcatgtc agaatgttct atgtcgtcag caacgataca
    11101 ctaaaagtca cgttcaccat gactgattgc tattcccagc acaattattt ctatacacgt
    11161 ttcatgattt gaataaacac tcacatcata catattacca ttcatacacc atactgcagc
    11221 ccctactata tggttgtaag ctcccaaatc tatatttgac ttgatcactt tggtagtaga
    11281 tctactaatt ctaaatccat tgctgataaa atttccattt tttcctcctt ctttctgcca
    11341 aaacagttaa aacaataata atctttatgt aaaaaagggc tgtgacaaaa gccttgtcac
    11401 agtcccatat tccgaataaa cggtggtcaa aagcttgcat ctgcggtaat aagttcaacc
    11461 aaacgaaaaa taagccaaca tttcacaaaa atttaagaag caattttcgt aaatttcttc
    11521 ttatttcttg aagctaacca cttctgttac aaccactttt agaacattta aagcttcgat
    11581 gtgacaaacc atctatgctg ctcgatcata aaattgatag caaaaattcc acaaagtaca
    11641 tatgtccccc atgaaaacaa aacattcata tgcggtacaa tgatctttgg aagaatcacg
    11701 atcaaaaatg atgccagtgc ccaaatccat cgattgattt gaatatgata tgtagctagc
    11761 atattcaata taaggtgact agccgttaat aaaaagataa aaacaaaaaa taaaatcagc
    11821 caatacattg tttcccacct tctaaaaacc aaacttagac agtctggact tcgttttctc
    11881 cacgactttg ttcatctgac agcatctttt tatcataagc ttttacaaat ggataataga
    11941 taataaatgc tatcaatgtg ttgatcatga tcaagataac aacacgccag tcatttcctg
    12001 ctgaaagata accgccgatt ggagctggta atgtccatgt tggcaaaaca gtaaatctat
    12061 tgaccatttc taatttgact gcaaaataac tgataatagt caaaactaaa ggaactaagt
    12121 tgaacgggat gaacatgtaa ggattcaaca tcataggagc tccaaaaatt atgggctcat
    12181 tgatattgaa gataccagga ataatagaaa aacgaccaac ctctttcaag aattttgatt
    12241 tacagaagaa tataaacaaa acagatacca cgatcgttgc tcctgcacca ccaattgtta
    12301 ccgtccattg ataaaactgt tcaggagcga tatagggtaa ttgggtggca gctgtaccat
    12361 ctgccaatgc ctttgcattc tcatccaaca tgatcagcca cattggacga acaacagccc
    12421 ctacaatact catgccatgg ataccaaatg accacaacaa atggatcaaa aacattggta
    12481 gtaaaacacc aagcaaatta ttccctaaaa tattggttaa aggctcgaat aacgataata
    12541 aggcagcgtt tacatcaaag cccgcgacgc ttcgaatcag ccaagtaatt gttaaaatga
    12601 ctgcacctgg aatcaatgcc tcaaaactac gggcaacagc aggtggcact tgttctggca
    12661 ttttgatagt aatatttcga tttttaaaga aacgatatac ttccactgca aaaatcatag
    12721 ataagatccc cgcaaacatc ccagatgctc ccaaagaact cataggtaag acccagccaa
    12781 gaggtgcctc ttcaggagtt aaaacgttta caggaacttg taacataaag aacgttccta
    12841 aagataatat accacctgtc acactatcta atccatatga tttagctaga tccgaaccca
    12901 taacaaaagt tgcataaata gccatcaatc ccatcgtcat cctaaagggt aaaacaatcg
    12961 ttgccgaata aggggcaata aattcattcc atccaggaaa cggtaaattg ataaagacca
    13021 taaaaaatgt cccaatcaaa atcaatgaca atgttgaaac aaccccatta cgaattgcta
    13081 ataaatgacg ctgtgtacca atttttgcca ttggtgggac aagtttctct tcgacccatt
    13141 tcatcagctt ttccactaaa accactcctt ttcaataaaa tatgaaccat tcattgcatg
    13201 acaacgcaaa gaatgttcat aactaattat ataattaaga aattttattt caatatattc
    13261 tcttaaataa tcaaatttat ttttaagaat cctgaaaata agttttctaa atcataggag
    13321 caacaaaaaa attcgataat taacaaaaat caaaatgatt aaatctagtg attgaaacat
    13381 atcatt
//
LOCUS       DANJLF010000067        13175 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000067 DANJLF010000000
VERSION     DANJLF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            12..84
                     /locus_tag="PWT25_002157"
     tRNA            12..84
                     /locus_tag="PWT25_002157"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="PWT25_002158"
     tRNA            88..160
                     /locus_tag="PWT25_002158"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="PWT25_002159"
     tRNA            168..249
                     /locus_tag="PWT25_002159"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="PWT25_002160"
     tRNA            267..339
                     /locus_tag="PWT25_002160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="PWT25_002161"
     tRNA            351..422
                     /locus_tag="PWT25_002161"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="PWT25_002162"
     tRNA            439..524
                     /locus_tag="PWT25_002162"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="PWT25_002163"
     tRNA            535..608
                     /locus_tag="PWT25_002163"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="PWT25_002164"
     tRNA            618..691
                     /locus_tag="PWT25_002164"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="PWT25_002165"
     tRNA            711..783
                     /locus_tag="PWT25_002165"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="PWT25_002166"
     tRNA            811..884
                     /locus_tag="PWT25_002166"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="PWT25_002167"
     tRNA            898..971
                     /locus_tag="PWT25_002167"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="PWT25_002168"
     tRNA            995..1084
                     /locus_tag="PWT25_002168"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="PWT25_002169"
     tRNA            1097..1170
                     /locus_tag="PWT25_002169"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="PWT25_002170"
     tRNA            1189..1264
                     /locus_tag="PWT25_002170"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="PWT25_002171"
     tRNA            1266..1336
                     /locus_tag="PWT25_002171"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="PWT25_002172"
     tRNA            1350..1423
                     /locus_tag="PWT25_002172"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="PWT25_002173"
     tRNA            1448..1535
                     /locus_tag="PWT25_002173"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="PWT25_002174"
     tRNA            1557..1628
                     /locus_tag="PWT25_002174"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="PWT25_002175"
     tRNA            1651..1740
                     /locus_tag="PWT25_002175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="PWT25_002176"
     tRNA            1753..1826
                     /locus_tag="PWT25_002176"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="PWT25_002177"
     tRNA            1845..1917
                     /locus_tag="PWT25_002177"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="PWT25_002178"
     tRNA            1921..1993
                     /locus_tag="PWT25_002178"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="PWT25_002179"
     tRNA            1998..2068
                     /locus_tag="PWT25_002179"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="PWT25_002180"
     tRNA            2081..2154
                     /locus_tag="PWT25_002180"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="PWT25_002181"
     tRNA            2166..2237
                     /locus_tag="PWT25_002181"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="PWT25_002182"
     CDS             2503..3696
                     /locus_tag="PWT25_002182"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL1022231.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="PWT25_002183"
     CDS             3826..5298
                     /locus_tag="PWT25_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1022232.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="PWT25_002184"
     CDS             5381..6169
                     /locus_tag="PWT25_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL1022233.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="PWT25_002185"
     CDS             complement(6236..6904)
                     /locus_tag="PWT25_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL1022234.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="PWT25_002186"
     CDS             7019..8731
                     /locus_tag="PWT25_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022235.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="PWT25_002187"
     CDS             8734..10503
                     /locus_tag="PWT25_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022236.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="PWT25_002188"
     CDS             complement(10708..11274)
                     /locus_tag="PWT25_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1022237.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="PWT25_002189"
     CDS             complement(11274..12230)
                     /locus_tag="PWT25_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL1022238.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="PWT25_002190"
     CDS             complement(12230..12886)
                     /locus_tag="PWT25_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL1022239.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT         3846 a         2464 c         2932 g         3933 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaa
//
LOCUS       DANJLF010000068        13157 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000068 DANJLF010000000
VERSION     DANJLF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            46..1785
                     /gene="yfcC"
                     /locus_tag="PWT25_002191"
     CDS             46..1785
                     /gene="yfcC"
                     /locus_tag="PWT25_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL1022240.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1942..3126
                     /gene="iadA"
                     /locus_tag="PWT25_002192"
     CDS             1942..3126
                     /gene="iadA"
                     /locus_tag="PWT25_002192"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL1022241.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3237..3440
                     /locus_tag="PWT25_002193"
     CDS             3237..3440
                     /locus_tag="PWT25_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL1022242.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3541..4203)
                     /locus_tag="PWT25_002194"
     CDS             complement(3541..4203)
                     /locus_tag="PWT25_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022243.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4216..5718)
                     /locus_tag="PWT25_002195"
     CDS             complement(4216..5718)
                     /locus_tag="PWT25_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1022244.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6266..7945
                     /locus_tag="PWT25_002196"
     CDS             6266..7945
                     /locus_tag="PWT25_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL1022245.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8072..8551
                     /locus_tag="PWT25_002197"
     CDS             8072..8551
                     /locus_tag="PWT25_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL1022246.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8770..9450
                     /locus_tag="PWT25_002198"
     CDS             8770..9450
                     /locus_tag="PWT25_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL1022247.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9458..10789
                     /locus_tag="PWT25_002199"
     CDS             9458..10789
                     /locus_tag="PWT25_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1022248.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10804..11766
                     /locus_tag="PWT25_002200"
     CDS             10804..11766
                     /locus_tag="PWT25_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL1022249.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11877..12215
                     /locus_tag="PWT25_002201"
     CDS             11877..12215
                     /locus_tag="PWT25_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL1022250.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12294..13001
                     /locus_tag="PWT25_002202"
     CDS             12294..13001
                     /locus_tag="PWT25_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL1022251.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4177 a         2328 c         2895 g         3757 t
ORIGIN      
        1 tttttattca tcaaaattac tgaaaaaaga aaggaagaaa taaatttgaa gaaaacaaat
       61 tcttcaggaa aaccgaaacg cgacttcact aaattatcga caccccatac gtatgtcatt
      121 atttttggtg tagtcatctt tgcatggata ctgacttttg tcgttccagc aggaaagttc
      181 agcacgcaag atattgaata caaagatgca aatggcgaaa caagtactcg tactgtttta
      241 cgtcaagatt ccttccgtta tgcttacgag ctggataagt cgtatgtgtt tgatcaatta
      301 gaagaactgc aagatcatcc agcagaacgt gaaaaactgg atgttccgga aaaaggattg
      361 gaaaaagtca ttgctgacgg agaaaaaaat cttacgcagg aaaaactaga tgaaatctcc
      421 ttgacagatg atgtgcttta cgatcagtac ggcgaaaata tctatgatac ttctaaaaaa
      481 ttgcataaaa cagccaaaat atggggaaca gatgattttg gcggctttgg tttcttaaat
      541 tttgtttttg aaggactagt atctggagat aagtatggat ctgctgtagg gatcgctgca
      601 ttgatccttg ttgttggagg tgcttttggt attattatgc gtaccggagc gatcgacgca
      661 ggtatctatg cgtttatcag taaaactaag ggactggagc gtttagcttt accgctatta
      721 ttctttgctt tttcattcgg tggcgctaca ttcgggatgg cagaagaggt aattccattc
      781 tcaatggtga tggttccatt tgtcattgcg ttaggatatg actcgatcgt agcagtaacc
      841 gtgacttatg tcgcatctca agtcggaaat gcgacctctt ggatgagccc gtttagcgta
      901 gcggtggctc aagggattgc tggaatcccc gtgttgtctg gtgcgacatt ccgattgatt
      961 atgtgggtag ttgttactgc attagccgct ggttatatga tgatatatgc agaaaaaatc
     1021 cgtaaaaaac cagaaagttc gttgacttac aaatcagatg attatttccg ttctcatatc
     1081 aaaaagacat ctgatgaaaa taaaaaattc atgcttgggc ataaattgat cttattagag
     1141 atgttagttg tccttgtctg gatcgtttgg ggcgtgactc aaaaaggcta ttacattcca
     1201 gaaatcgctt ctcaattctt tgtgatggga ttagttgctg gaatcattgc tgttcttttc
     1261 aaattagatg gaatgaaagt caatgacatc gcttcttctt tccaatctgg tgcagctgat
     1321 ttagctggta cagcaatcgt agtaggtatg gccaaaggga ttcttcttgt cttgggagga
     1381 tctgatgcat ctgttccttc agctttgaat acgattttgc atgggatcgg gacggcgttg
     1441 accggtgttc cagctgtcat tggtgcttgg gcgatgtaca tcttccaaag tttgttcaac
     1501 ttggttgtta catcaaattc aggacaagca gctttgacta tgccaatcat ggctccactg
     1561 gcagatttag taggtgtatc cagacaagta gcagttcttg cttatcagct tggttcaggt
     1621 tttatggacg cttttacacc cgtatcagcc agcttgattg gtgtgctagg tgttgcacgg
     1681 atcgaatggg caaaatgggc aagattccaa atcaagatgc aaggattcct gtttgtttta
     1741 ggaactattt tcatcatgat tgccattgca ataggtctgc agtagtactg gagacctgta
     1801 aataaaaaag taatccaaac agtagataag gactccaaga accgtcattt tacggtaaaa
     1861 tcaaaggagt aaagatccta tcgtttggat tattttttaa gatcctatca tcagttatac
     1921 ttaaagaaag aggaaaaatg tatgaaaatc attcgacaaa ttgaagtgtt tgcaccagat
     1981 tacttaggaa aaatggatgt actgattgcc ggtgggaaga ttttggctgt agaagaacag
     2041 ctagaaggcg gctacgaagg agtggaagta gaagaacttt caggtgaggg aaaagtacta
     2101 accccgggtt tcattgactg tcatttccat attttaggtg gaggaggaga aggcggttat
     2161 caaaatcgaa cgccagaagt cacattgagc caattgacta cagctggtgt aacgacagtt
     2221 gtcggttgtt tgggaacaga cggagaaggt agagatatga ctgcgttgat cagcaaagcg
     2281 aaaggtctgg aagcagaagg aatctccact tatgtatacg aaggttcgta ccgattgccg
     2341 gtaaaaacag taacagattc tatcatcaaa gactttttga caattgataa aattatcgga
     2401 atcggagaaa tcgcagtatc cgatcaccgt tcttctcagc caagttttga agagttcgct
     2461 cgtgcagtag cagatgctcg tgtaggaggc atgttatccg gaaaagctgg gatcatcaat
     2521 gtccacttag gcggaggaaa aagaaagcta gagctattga cacgaatcgt agaagaaaca
     2581 gaaattccga tcacacaatt tttgcctact catgcaaatc gcacaccaga attatttgaa
     2641 gcttgcgtgg catttgccaa acgtggtggc acgattgact tcactgctag cgaagatcct
     2701 gatttttggg agaagacaga tggtgaagtt cgcttcagca aagcactgaa acgattgatc
     2761 gaggaagatg tttctttaga taatttcacg atgacctcag atggacaagg aagcttgcct
     2821 tattttgatg aaaacaatca tttcctaggg ttaggagtag gaagtgccaa ggcattgcta
     2881 gtagggatca aagaagctgt gcaaaaagaa agtatccctt tggaaatcgc cttacgagct
     2941 ataaccagta atccagcacg tatattgaaa ttggataaaa aaggaaaaat cgaaattggt
     3001 gctgatgcag atctgtgtat cttggataaa gaaacgcttg atattgatac agttatcgct
     3061 aaaggagaaa taatggtcca agaaaaagaa gtcaaagtat gggggacttt cgaaaaaagt
     3121 ttttaatcaa cagctctaac tatctttttt agaaaacgag cgttctgttt gtctgtatct
     3181 gttgttgatg ttaccctgaa ggtagatcaa taaacaaact aagaggagga agcacaatgg
     3241 cagatttaaa aggacgttct aaagatatga aagataaagt agtcggtgaa ggaaaagaag
     3301 catacggcaa agcaacagac gacaagagca aagaaacaga agggaaagca caatcactag
     3361 gtgctgacat caaagaaaaa gctcgaaatt taggcgacga agtcaaagac aagttttctc
     3421 gaaaagacga aaaaaaataa aaaccactta ttactaattg aatcaagcaa ataaaaaatc
     3481 cgaaccatat ctgcgagaca gatatggttc ggatttttta tttgatcatt atcctgtttt
     3541 ttaaacttcg attttttgaa attttttatt ttttagttca aagacttcat cacagctttt
     3601 tgctacttca atctcatgag tcacgatgat aatacagcga ttttgttcat gggcgatttc
     3661 ttggaaaagt ttgacgatat cttgtgaagt tgtttcatct agatttccag taggctcatc
     3721 cgctacgatt agttgatgtt cacagcagat agcacgaacg atagcgactc gctgctgctg
     3781 acctcccgaa agtttggtca cctgtttttt tgctaatatt tcatcaattc ctacacgagc
     3841 cagtcgatct aaagcaactt gtcgataatt ctcttgcttg gtcttagtga tttccattgc
     3901 tgctatgacg ttgtctagag ccgataaata aggaagcaag ttgtaagctt ggaaaataat
     3961 cgagacatca tttttacgat aacttcttaa cccgattttg ttcagtgaac gatcttgata
     4021 gtataccttt ccttcttttg gtgcatctag tccagaaata agcgaaagaa aagtcgtttt
     4081 tccagaacca cttgctccta aaatagcgta catttttcca gcttcgaaat tcagttctac
     4141 atctttataa agcgcatctt gcggatcttg ataccaaaat cccaattgtt ctgtccgtaa
     4201 catcaaattc ctcctttagt ttaataggat ttttcttggg tttaatctta atatccctat
     4261 agaagctaaa aagacagaaa acagactgat tccaaatgct actccggcta atagtcccaa
     4321 ttgtgcaggt ttgacattga tttccaattc gctgacttga tcagaaacag caaatggatt
     4381 attcgtggtt ccttgttgac catcgcgttg accgccttgt tcttgtctgt tacctggcat
     4441 ttgtccaccg ccaggagctt gctggttttg tgtcgtggtt tctgtctgtt ggtcaagtag
     4501 ctgctgtccc acggcattcg cgacgacgtt tccgctaagc gaagcaactc ctaatgcaag
     4561 aatcatacaa atagccactt cggtaaacaa ttggagaatg attttacttc tggattctcc
     4621 taaagatagc aagacgccta actcatgccg acgctcccga atggtaatca taatgatcag
     4681 agtaagtatg ataataccgg ctacagccac tagtaaaaca acatttttag aaaaacttgc
     4741 cacgttgttt aaaggttcca acattgactg atacatggaa tcatttgatt gtaagctaaa
     4801 tgtatctgtg tcgatcagct tctcagcttc tttgagaaac tcatccattt tatctggatc
     4861 agatagagtg taaatagcag aatctatcgt gttatcgtca gatgtcccat ttagttcatt
     4921 tgcaaaagtg taagaagtgt aaagtgtatt cgacggattc atgaagttga atttcatacc
     4981 catactgccg cttgtttcgc tggattcata aattcctttg atcgtcattt cataagtggt
     5041 atcttcatct tcagtactcg tgattgtgaa agtatctccg actgccaggt catttgtttc
     5101 tgctagagta gaatcaatga ctacattgtt tgtgcctttg tcgctgtcat ctattccttc
     5161 accatccgtg atttttgctg ttccatcaga aaaattacta ttttgtgcag ttgctgacac
     5221 accactgatt tgaaagtctg cttgagtcat ttgcggattc ccgcccattc ctccagcagg
     5281 ttcaccgcct gcatcagtag tttctgaact gtcttcactt gaatcagaac tagtgatggc
     5341 agttattcca tcttttgcca tagctgaggc ggaactttca aaggaataac tctttacatt
     5401 atctaattca gctatttttt ccgcgtccgt taaggaaact ggtgtaagag aaaaactgcc
     5461 tggatctgga cgttcttcag tggtagaacc gcttgaagaa gtttgttcct gttttttaaa
     5521 tgaagcttct cgattggcac ttaaagtcac tgttgctcca acttcttttt gtgcttgatt
     5581 cgctgcttct tctgcagcgc ttcgaatcgt gaggccagct agcacaaaaa taagaatggc
     5641 tgtgaacacg gcaaacaata acaatgaacg tccccatttt acttttgtac ttttccatgc
     5701 acgtttcata aaattcatat tttgcatccc tcctagtaga tgcatttgat aatagacaac
     5761 ctatcttaaa gctagcttaa atcttatgct gtttgcacac catcaacatg aggttctgta
     5821 tatataaaaa gtcactatag ttattgcaaa aaatgtggta aaatgaaaaa agtatgtttc
     5881 aaaccgacga agaagaaatg aaagaggaat agcaaaagaa atcctagatg ctacagaggg
     5941 atttttacgt gcatagttgt tctaacaatt gctgatacta gcgatttagt ttatttgttg
     6001 tataataatg gggaacagct gaatacttat ataagtattc tggctatagc gtttcgcaaa
     6061 agatccattt tgttaatgaa gtatatccaa aataaaatgc ccatccaact gtcagaacgc
     6121 ggatgtgctg gtaattttcc tgctgattct ccataagttg agaaatagtg attttgcata
     6181 tgcttcattg gaatggctca tatatttaga taaagaagaa aatcgacatc tttaaacttg
     6241 gatgtcataa gaagaggtga agtttgtgag taccattaaa attatcccat taggcggcgt
     6301 tcgtgaaaat ggaaaaaaca tgtatgtcgc agaagtgggc gaggatattt ttattttaga
     6361 ttgtgggcta aaatatcctg aaaatgagtt gttggggatc gacgtcgtaa tccctgattt
     6421 tacgtattta gaagaaaaca ttgatcgtgt agctggtgtc tttttaacac atggacatgc
     6481 agatgcgatc ggggcacttc cttacttgtt gtcaaaaatt tctgtaccag tattcgggac
     6541 agagttgacg atcgagttag caaaattgaa tgtcagtcgt aatgaagcag ccaaaggatt
     6601 taaggatttc catgtggtag atgagcatac agaaatagac tttggtgaaa cagtagtgag
     6661 ctttttccgt accactcata cgataccaga ttcagtgggg atcaatctga aaacaaaaga
     6721 aggtagtatc gtttatacgg gagattttaa atttgaccaa acggctatcc caatgtatca
     6781 aacagattat gcacgattgg cggaaatcgg caaagaagga gtcttagcct tactaagtga
     6841 atcggcaaat gcagaaaatc ctcagcctgt agcttctgaa gcccaaatag cagatgaagt
     6901 atttgatacg atcaaatatt gggaagggcg tatcatcgtc gcttgtgtag caagtaattt
     6961 gcaacgagtg caacaagtat tggatgcagc agaaaaagca caacgaaaag ttgtcttgac
     7021 tggtcaagat ttcgaacgaa ttattcgcac agcgatgcgt ttagaaaaac ttcaattacc
     7081 aagtgaagat ctgcttgtta cgttgaaaga catgaaaaac tatgatccag aagaactgat
     7141 cattttagaa actggccgaa tgggtgaacc catcaagtct ctgcaaaaaa tggcaaacgg
     7201 tacacaccgt aatgtcaaga ttgaagaagg agatttggtt tatatcacta ctacgccaag
     7261 catcgcgatg gaaaccatcg tggcgaaaac ggaagatatc atttatcgag ctggtggaac
     7321 agtgaagttg atctctgaga atatgcgcgt ttctggccat gccaatccta atgacttgca
     7381 attaatgatc aatttgatga aaccaacata ttttgtgcca gttcaaggag agtatcgtga
     7441 attagctgct catgcagatt tggctcatgc agtagggatg ccatacaaaa atatctatat
     7501 tactggacgc ggagacgtgc tggaatataa gaacaagcgt atgagtgttg caggaactac
     7561 aagtgcagaa aatgtaatga ttgatggatt aggcataggt gatatcggaa acattgtttt
     7621 acgtgatcga aaaatcttgt cagaagacgg gattttcgta gcggttgtca ctatcagtcg
     7681 gcgagaaaag aaaatcattt caaaacctca aattacttca cggggattcg tttacgtaaa
     7741 agctagccgt gatctgatga aagaaagcag caatatgatt gaaacaatcg ttgagaaaca
     7801 tttagaaagc aatgattttg agtggagcaa attaaagcaa gacatccgag atcaattagg
     7861 ccgttactta tttgaacaaa caaaacgccg cccagtgatc ttgccagtca ttatggaagc
     7921 tactcaacgt cgaaataaaa aataaacaaa aagagtaaga tgaacctctc tatagagtgc
     7981 tcgtcttgct ctttttttgc gaaaaacaaa taatttttga atacagcatc cctcgcttta
     8041 taataaacga tagaataaag ggagtgaatc aatgatgagt caaacaaaag tcgaacgtta
     8101 tttagaagaa caaaagatca gctatcaacc acttgatttt agtcatgtgg tagaagaatc
     8161 tttcacagaa gaactacaaa aaagagggat tgatccagct ttgatatgta aaactctcgt
     8221 agtgaaagga aacaaaactg gggtgatcat cgcattgatt cctttgaacg atcacttaga
     8281 ttacaaaaaa acgagacaag tgacgaaaga tcgtaaagtt ggttttcctg gaatggattt
     8341 tgtattaatg catacaggat atccccacgg agcaaacacc cctattggaa taaaactcgc
     8401 acatcctgat tattgttttt tagcagacag ttctttaaaa gaaaaagaag aagtaattgt
     8461 gtcaagcggt gaaatcggga gaagccttcg gataaaagta gccgatttag aaaagatcgt
     8521 ccatcctatt tacgctgatt taattagtta agggacgaga agagaagcta agcagagcag
     8581 agacctcaaa cttgagatta tatctgcttg gtacttggtg catcctgtat tatctgttac
     8641 aattgctttg tacatactgt actatcaatc caatgaaaat atatttagca gctatatttt
     8701 ttcattgatg tgcaagtgat gaaatcgttt ttaataaaaa agatacagag aaaacgcgag
     8761 gtgtaaaata tgaaaattac gtatcatgga cattcctgtt taacaattga aatggaaaat
     8821 ggccaacgcg tgatgattga cccgtttatt acaggaaatc cattgacaga cttaactgca
     8881 gaagaagtaa agacagattg gttgctgatt actcatggtc atagtgacca cataggagat
     8941 atgattccta ttgcaaaaga aaatcaagcc acaattatca gtattgttga gattgctgat
     9001 tatgcgaaca gcagaggagt agattcattc ggtatgaata tcggtggaaa acatgcgttt
     9061 ccattcggcg tggtaaaatt cgtacatgca cagcatagtt caagctatga agtagacggt
     9121 atcgttcagt atatgggcga gcctagcgga attattattc aagcagaagg aaaaacgatt
     9181 tatcatgcag gggatactgc ttatttcagt gatctaggat tgcttgcaga agaattcgat
     9241 attgatgtcg ctttcttgcc gattggcgat aactatacga tggggccaga agatgcagca
     9301 aaggctgcgc aaacaattcg tgccaagaaa gtagttccaa tccattacaa tactttccct
     9361 gtcattgaac aagatcccga atcttttgta gagttactgg aagatagtga aggaaaaata
     9421 atgagcgtct cggaaacgct ggaagtgtag gcagaaaatg gcgacaaaac atgatttgat
     9481 tttagaatat attgaaagtt tacctgttgg caatcgaatt tctgtaagaa gcatcgcaaa
     9541 agaattgaat gtcagtgaag gaacagcgta ccgtgcaatc aaagatgcag aaaatgtcgg
     9601 actagtttca acaattcaac gagtagggac catccgtatc gaacgcaaat taaaaaaaca
     9661 tattgaacgt ttgacatttg gcgaggtcgt caggatcatt gaaggggacg ttttaggcgg
     9721 ggcggcagga ttagacaagg tcttgaataa attcgtcatc ggcgcgatga ctaaacatgc
     9781 catggaacgc tatattacgc caggatcact aatgatcgtc ggcaaccggg cggaagtcca
     9841 aaaattagca ttggaagatg gcgccgcagt attgatcaca ggaggattca atacgactgc
     9901 tgagattgca aagctagctg atgatcttga aatgccgatc ttacgcacct cttatgatac
     9961 atttacagtc gcaaccatga tcaaccgagc gttaagcgac caattgatca aaaaagatat
    10021 catgcttgtc agtgatatct atatgccagc tgaaaagact cattatttgc agcagacaga
    10081 tacgatttct gattatcagc gtttgtcaga agaaacgcag cattcgcgtt ttccagtcgt
    10141 caaccgccat catcgtttga tggggattgt tacggcaaaa gatgttttag ggaaatcgcc
    10201 aaaccagatc atcgatcggg tgatgacgaa agaaccaatc agtgtaaaga aaacaatgag
    10261 tattgcctct gttagccatc aaatgatctg ggatgggcta gaagtcatgc cggtcgtttc
    10321 cgatgacttg acattggaag gcttgattac ccggcaagat gtaatgaaag cgatgcagct
    10381 tgtccagcga caaccgcaga ttgcagatac gatttcggat cagatatctg gaaatatcca
    10441 tacgattgat actgaccgtg aaggaaatcg tttggaaaac cctaagttca aattcgtagt
    10501 agcaccacaa atggtcaatg gcgttggaac catttctttt ggcgtactta gcgaaatcat
    10561 ttctgatgtc gcacaaaaaa caatggtgat gaaccaaaaa cgaaatatct tgattgaaca
    10621 agtgaaccta cattatttgc ggctgatcca attggaaagc gaattagaca ttcgtccacg
    10681 tgtattggaa atcggcagac gttccgctaa actggatatc gaagtgttta ttgaaaacac
    10741 catcgtttcc aaagcaatcg ttgtttgtca agtaatggaa cgttcttaga aagcaggagt
    10801 gaaatgacag tacagcaaga aatagtagat acaataaaaa aatacgataa aatcattatc
    10861 cacaggcatt tgcgccctga tcctgatgca ttaggatcac aagtaggatt agccgaacta
    10921 ttacgggaaa gtttcccaga aaaagaaata cgtcaagtag gtgaaacgat tgaagggctt
    10981 cgctttttag cagaaatgca agaagttgaa gatgtcttct accaaggagc gctagtcatc
    11041 gtgactgata cagctaattc acccagaatc agtgatcaaa gatatcagtt ggcagaaaaa
    11101 ctgatcaaaa tcgaccatca tcccaatgat gacccttatg gagatcttgt gtgggtaaat
    11161 acaaaagcaa gtagctgtag tgagatgatt gcagaatttg ctttgatgtt tcctgaatct
    11221 ttaaagatga acgttaaggc tgcacggtta ctttatgcag gaatcgtcgg agatacaggg
    11281 cgctttttgt atccctccac gacgtcccgt acattagaga ttgcttcgat attgagaaat
    11341 tatccatttg atgcaagtgc tttgaacaga gagattgaac aaatgccgat gaaagtcgct
    11401 aaactttccg gttatcttta tcaaaatatc caggtggatg aaaatggtgc aggaagaata
    11461 atccttccgc aagaaattct tgataaatat ggaatcgatg attcagagac agcttcgatc
    11521 gtttcacttc ctggagtgat tgacgaagtg ctggcttggg ggatttttgt tcagcagcct
    11581 tcagggtatt accgcgtacg tttacgttct aaggggccga tcatcaatga attggctaaa
    11641 cgccatcatg gaggaggcca tccattagca agtggtgcga atgcaaaaga tttagcagaa
    11701 gtagaggaaa tctataaaga gatccaagat atatgccaat cgtataagag cagaaaaaca
    11761 gaatgatact tcgttcgaaa acgtgccttt tgtagtaaaa atcacaaatg tatcttttca
    11821 agatcttctt tttccgttac aatagtgctc actaaagatt ttaaaggaga aagaagatga
    11881 cattaccaaa ttgcccagaa tgtggctcag agtacacata tgaagatcga ggcttgttga
    11941 tttgcccaga atgcgggaac gaatggaaca aagaagaaca agaaactgtc atagaagacg
    12001 gattagtggt attagatgca aatggaaacc gtctgttaga gggcgatagc gtgacagtga
    12061 ttaaagactt gaaagtaaaa ggtgcgtcag gtgccattaa acaaggaaca aaagtcaaga
    12121 acatccgttt agtagaaggc gatcataata tcgattgcaa ggtagaagga tttggaccga
    12181 tgaaactgaa atcggaattt gtgaaaaaga attaaagaat attcaaggtc ttttcacatt
    12241 cttttcctac tactaatgat aaaataaagt aaacgaaaag gagtgaaacg aaaatgtatg
    12301 gttatggaat gtatggtttt ttagatccga catttctttt ggtcattctt ggattagtta
    12361 tttccggaat tgcgtcagcg tacgtcaata gtactttccg aaaatacgat caagtgagaa
    12421 gcaaaaacaa tgtgactggt acacaagcag cacaatatat attacaaagt caacagataa
    12481 ataatgtcgg tgttcaacaa attgccggag atctcacaga taactacaat tcaggtaata
    12541 agatgttgag tttgtctcaa gcaactgcac agtctacttc agttgctgcg ataggtgtag
    12601 cagctcatga atgcggacat gcggtacaag atgcttcggg ttatgtccca ttgaagattc
    12661 gtgcagcact tgttccagca gctaactttg gttcgatgat ttcgttcccc ttgattatgg
    12721 taggtgtatt gcttagctgg aactctacgt taatcaatat cgggattttc gctttttctt
    12781 tagcattact tttccaattg gtgacattac cagtcgaatt taacgcgtct cggcgagcaa
    12841 tccaaatttt gagtgaaggc ggactactga cggaagaaga agtaccgatg gcaagacacg
    12901 tattatttgc agcagcactg acttatgttg cagcagcgct ttcgacattc ctgcagctgt
    12961 tgcgcttgat cttattgttc ggcggcaacc gaagagatta aaataaagaa gttgtgaaat
    13021 cagcgctttg atctgagcta ataccgagag ttagctgatt gaataccatt tatttggaat
    13081 aagggctctt tgtcaataag gactgatggg ttgtacaaaa tcaaatctgg gtcagaatga
    13141 acctcattct ggctcag
//
LOCUS       DANJLF010000069        12905 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000069 DANJLF010000000
VERSION     DANJLF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            127..3654
                     /locus_tag="PWT25_002203"
     CDS             127..3654
                     /locus_tag="PWT25_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL1022252.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="PWT25_002204"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="PWT25_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL1022253.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="PWT25_002205"
     CDS             7442..7783
                     /locus_tag="PWT25_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1022254.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="PWT25_002206"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="PWT25_002206"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL1022255.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="PWT25_002207"
     CDS             9140..11560
                     /locus_tag="PWT25_002207"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL1022256.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="PWT25_002208"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="PWT25_002208"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL1022257.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4385 a         2243 c         2738 g         3539 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaact
//
LOCUS       DANJLF010000070        12734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000070 DANJLF010000000
VERSION     DANJLF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PWT25_002209"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PWT25_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL1022258.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="PWT25_002210"
     CDS             complement(322..891)
                     /locus_tag="PWT25_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL1022259.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="PWT25_002211"
     CDS             1077..1979
                     /locus_tag="PWT25_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1022260.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PWT25_002212"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PWT25_002212"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL1022261.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PWT25_002213"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PWT25_002213"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL1022262.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PWT25_002214"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PWT25_002214"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL1022263.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="PWT25_002215"
     CDS             8547..8732
                     /locus_tag="PWT25_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL1022264.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="PWT25_002216"
     CDS             complement(8794..9990)
                     /locus_tag="PWT25_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL1022265.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="PWT25_002217"
     CDS             complement(10160..11596)
                     /locus_tag="PWT25_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL1022266.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="PWT25_002218"
     CDS             11889..12698
                     /locus_tag="PWT25_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1022267.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DANJLF010000071        12506 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000071 DANJLF010000000
VERSION     DANJLF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            18..491
                     /locus_tag="PWT25_002219"
     CDS             18..491
                     /locus_tag="PWT25_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL1022268.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            500..727
                     /locus_tag="PWT25_002220"
     CDS             500..727
                     /locus_tag="PWT25_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1022269.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1363..1734
                     /locus_tag="PWT25_002221"
     CDS             1363..1734
                     /locus_tag="PWT25_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022270.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1990..2232)
                     /locus_tag="PWT25_002222"
     CDS             complement(1990..2232)
                     /locus_tag="PWT25_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1022271.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2264..3166)
                     /locus_tag="PWT25_002223"
     CDS             complement(2264..3166)
                     /locus_tag="PWT25_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1022272.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3179..3367)
                     /locus_tag="PWT25_002224"
     CDS             complement(3179..3367)
                     /locus_tag="PWT25_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL1022273.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3381..3944)
                     /gene="amaP"
                     /locus_tag="PWT25_002225"
     CDS             complement(3381..3944)
                     /gene="amaP"
                     /locus_tag="PWT25_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL1022274.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3982..4875)
                     /locus_tag="PWT25_002226"
     CDS             complement(3982..4875)
                     /locus_tag="PWT25_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1022275.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4953..5876)
                     /locus_tag="PWT25_002227"
     CDS             complement(4953..5876)
                     /locus_tag="PWT25_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL1022276.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5925..6275)
                     /locus_tag="PWT25_002228"
     CDS             complement(5925..6275)
                     /locus_tag="PWT25_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL1022277.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6308..7210)
                     /locus_tag="PWT25_002229"
     CDS             complement(6308..7210)
                     /locus_tag="PWT25_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL1022278.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7203..8060)
                     /locus_tag="PWT25_002230"
     CDS             complement(7203..8060)
                     /locus_tag="PWT25_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL1022279.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8394..9203
                     /locus_tag="PWT25_002231"
     CDS             8394..9203
                     /locus_tag="PWT25_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1022280.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9243..9740
                     /locus_tag="PWT25_002232"
     CDS             9243..9740
                     /locus_tag="PWT25_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1022281.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10125..10198)
                     /locus_tag="PWT25_002233"
     tRNA            complement(10125..10198)
                     /locus_tag="PWT25_002233"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10162..10164),aa:Ile,seq:cat)
     gene            10385..10708
                     /locus_tag="PWT25_002234"
     CDS             10385..10708
                     /locus_tag="PWT25_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022282.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10872..11126
                     /locus_tag="PWT25_002235"
     CDS             10872..11126
                     /locus_tag="PWT25_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022283.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11196..11441
                     /locus_tag="PWT25_002236"
     CDS             11196..11441
                     /locus_tag="PWT25_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1022284.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11517..11882
                     /locus_tag="PWT25_002237"
     CDS             11517..11882
                     /locus_tag="PWT25_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022285.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11943..12506)
                     /locus_tag="PWT25_002238"
     CDS             complement(11943..12506)
                     /locus_tag="PWT25_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022286.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         3606 a         2332 c         2133 g         4435 t
ORIGIN      
        1 ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta
       61 taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa
      121 aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc
      181 tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc
      241 aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta
      301 ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg
      361 ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat
      421 taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt
      481 tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa
      541 aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat
      601 tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt
      661 tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga
      721 agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga
      781 gacttttaga agttatttac gtctattatt cagatttttt atttcgattg aataccgttt
      841 tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact
      901 atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat
      961 aatactttat tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt
     1021 attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg
     1081 tgttaactct tactccattc ttttttagta agctcccata tatactgtaa agccttctat
     1141 atattatgtc atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata
     1201 acagtgatgg acaattcttt tttattttta acattatttt gataataatg attatttatt
     1261 taaaaataca ttagataaca tttgttacga cacgaaaaag cttcacaaac aaatatcgtt
     1321 attttaaaat gagggcgaac tcacacaatt aggaggaata gtatgccatt tattgtttac
     1381 ttttttcttt cattactcgt gatctatatt ccattaccaa catttttcct gctcgtcaat
     1441 cgtctagcag agcaacatga tacgattgct gctcttacga aactttgttt acttctattt
     1501 gtttgtctag attactttac gagctattat tggctaaaac agacaagagt aaaaaagaga
     1561 tattatttct tccttgtctt tttcaatctc tttgagtttc tgatccattg ctttttactt
     1621 catcagtatc atacgatcca tttgtggatc atctgggttt tcaaagtggt cgtattattt
     1681 tgtatgttca cctttcctat ctcaagaaaa tttagggagg cattgtttta ttaaagaagc
     1741 ttctctgtat caataaagac acatttaagg aaaaactttt aaaaatctaa ttgggaattt
     1801 gcccgcataa ataagaggct gtgacaaaag tcttgtcaca gcctcttatt ccgtataaac
     1861 agtgttcaaa agcttgcatc ttcgataata tgcccaacca aatgaaaaat atgagaagct
     1921 atttttgttt ggctgttctt attacttgat gaagaacagc tttttcacga cctcttttta
     1981 aaagtctttt tacagttttc gagcaaagaa tgatacgatt agtactaaaa tgatggcacc
     2041 aatcaatgat ggcacgattg ccattcctgc aatacttggt ccccaagaac ctaataatgc
     2101 ttgaccaagt gctgatccaa ctaaacctgc tacgacattt gcaataatcc ccatcgaaga
     2161 acctttattc gtgattgctc ctgcaattgc tccgataatt cctccgacga taagtgacca
     2221 aataaatccc atgtttcgtt cctcctaatg ttttacttcc ttattactga acccgtggtt
     2281 ctgtattttc ttttactttt tgtgcgccgc ttccagctgc tgattttact ttatctgttt
     2341 gattcgaagc aaattcaccg gtttttttcg ctgcgtcagt tactttgtct tgcaccgttt
     2401 cttgatcttt ttcaaattct tcttttgttt tgatgtccac tacgttagcg ttgacttcga
     2461 tcacttctaa atgtgtcata ttttttactt ctttgccgat tacttcttgc atttcttcaa
     2521 agattttttc gatatctttg ccatattcca caatgacatc catatcaact gctacttgtt
     2581 ttttaccgac ttctgtttcg atcccagccg ttacattatc tgtattcaca agttttcctg
     2641 ccacattaga gaagaaacca ccgtttgcag ataataaacc gtctacttgt tcaattgcga
     2701 ttccgatgat tttttgcaca actttatctt cataattcag ctcaccgcgc attgaatctt
     2761 cctgttcaga atcatgcttt ccttggttat ttgtatgatc agaaccaagt tttccttgtc
     2821 cgtttgtgtg gtcagaacta agtttttctt gtgctttcgc atggtcagaa ggatgttttt
     2881 gctcttccac aggtttagaa ggatcgattg catcatgttg ttctggaact ttagattctg
     2941 gagttcctgg atgatgaggt gcatgctttt ctgcatcatg tttcttctct tctactcctt
     3001 gttgaacgtt agggactact ggatctggat gtgcagatac tggtgctgtt ttgttctttt
     3061 catcgttcac accatttgat tggtttggtg tcgttgggtc tggatgtgca gacactggtt
     3121 tttggttgtt cattttattg ttattatttt tgttattgtt attcatggat gtttcctcct
     3181 aagatttaaa atagtgatct aagattttcg tttgtttcag ataaaaaccg atagcgacgc
     3241 cgatcgctgc catgatcaag atcaataagg tcttgaaaaa gcccacggtg atccataaaa
     3301 cggctaatat caatccaagt accccaaaga tgatagggag cttgtatgtc gaaaatagtt
     3361 ctttcatttt tggtcccccc ctattctact cgtttgcggt cattgcttcg tttagctggt
     3421 tcttcaaagc ctgtgtattt caccgatact ttcactggct cttttgttcc caatatgtgt
     3481 tgtacttcat tttcgatttc tttcatcaat gttcctgttt taccaatgag tgctgaagtt
     3541 cgtttcaact ccccttttac attgacggcg atcttgtttt tcgttgcttt gacatttact
     3601 tttggtgaat ggatgaattc atcttcatgc aagtgagaac gaaccattcc ttcaattgct
     3661 tttttatcca acgtcaactc cccatcacca tgtttcagta gaaagtttcc gattgttttt
     3721 ggataaaaca ggaaaacaag taacgtgata attgtcaaaa tagcaaatgc aacggcaagc
     3781 caaaatacat attgttgcac gtatgaatta gtgatggaaa agaatcgaaa cttctctagc
     3841 tgaaaaggca atggataggt tgcacttaat agtatcgcaa acaaaagcac agatagcaaa
     3901 agtaatgttc cgatcactcc gatcgttttt acacctttat tcattttcgc acctcctcat
     3961 aggggtgaaa cgatcaactt cttataagtt gatcgcttct ccaccagtta ctccgtaaat
     4021 ttgggctgta acataacttg ctttattcga tgctaagaaa acataaactg gtgctaattc
     4081 tcctggctga cctgcacgtt ctagtaaact ttgctgacca aattcaggga tcgaatcaga
     4141 aggttgtcct tcgtctagtt gtaatggtgt ccagattgga ccaggtgcta caccatttac
     4201 acggattcct ttaggtgcaa gctgttttgc taaaccgatc gtaaagttag cgattgaagc
     4261 ttttgttgct gcgtaatcta ataaatgttc gcttggattg aatgcttgaa cagaagttgt
     4321 tgttacgatt gctcctcctg ctgggatatg cggaacagct tctttcacta aagcaaacat
     4381 agcgatgatg tttatcataa atgtgtcttt gacttgctca agtggtaact cagcaataga
     4441 ctgacaagaa atttgctgtc ctgcgtttag taccatcgta tctagcccgc caagttcact
     4501 cacggcttta tctatgattt ctttaggtgc ttgttcgtca cgaagatcaa aaggtagtaa
     4561 gacaatctta cgtcctgcat ctttcacgta ttttgctact tcctctgcat ctttttcttc
     4621 tcctgggaag aattgaagag cgatatctgc cccttctcta gcaaatgcaa tcgctgctgc
     4681 acgaccgatc cctgaatctc caccagtgat tagtacacgg cggttttcta gttgattatt
     4741 gccttcatag gattcttctc cacaatcagg aacaggaatc atattctgct gtaaagctgg
     4801 tgtatcctga tcttgtttag gaaatttctc cgtatggtac aagtttcgcg ggtcgattaa
     4861 ttttggttct gtcattgctg tattcctcct agtttttaaa ttgtattgcc aaaacaaatt
     4921 tctttgtttg atacctgttt taaatagatt ctttaatttg tataatcttt ccggtataaa
     4981 tggtaaccta aaactactgc tgctatcaaa gagccaaaga ctacgagtat aaagcctaga
     5041 gcagaacttt tacccggtaa tcctcctgta ttcattcccc aaatccctgc gaacaacgcc
     5101 ggaacggaaa tgattaatgc agctgaatct agatatttca tgatatgatt tagattattg
     5161 tccatcatcc cgttgaatag atcaccaata ctctctaata aatctcgata gatctcgatc
     5221 attttttctg tttgtcgctg tctcaaatgg acatcataga gtagctgttc atcgtctaaa
     5281 cgtttgatga aagtttcaga attccataat tgatccattg tttcccgttg atctcttaag
     5341 gtatgatcga tatatacgat gtctctttca agatctgcct gtttatacag ttcttcattt
     5401 tccgttgttt tacgagctgc tttatccaaa gcgtcaattc gctttttcat ctctcttagt
     5461 tctttcacgt aatgggtgta aatatctaat aatgtatacg tgatgatctt ttcaaacgag
     5521 tttaaatgat ttccatgctt tgtcagcagc cgatccacaa attcggtttt tctatcgata
     5581 caagtaatca agagattatc ggaaagtaca aaagatattg gcgttaattt tttttcgata
     5641 cgattatctg actgatctag attaatcact accaaagaaa atggatgctc tagtttcgtc
     5701 tcataaaggc gctccatccg agaaacttct tccggatatt cggttcctac gaaaatatca
     5761 tcaggaagct cgaattcttc gattgcttgc tcaatctctt cgggctttgc actatcaagt
     5821 acgagccaat tatatttttt ttcatctgtt tctattacat tcttttcttc atccacaaag
     5881 tagtatctca atgtatacct cctttttgga ctttctcgaa actattagct gaagaagcag
     5941 ttctttaatt tgatattata atcgagtact gcgatttcat tttctaatga aaagctgtca
     6001 agctgtttgt tgaagtccat gttcaatcgt ttaccgaaat aattttgctc taatttagat
     6061 gtatagatgt ccaataatgg atgctcgatt ttagtcgcat attctccaaa ttccgaaaca
     6121 aaaggaatga tttgaaaaaa gctgcctttt gcaaaagcac ctttgtccct tagaaatgga
     6181 tcggaatcat tgataagcgc gagtgcgatt tttgtatttt ccccaacctt ttcaattaac
     6241 gtctctactg catttttaga cattgtaatt tccattccta ctccccctct tcagttgttg
     6301 tcttctatta tttaacaact acatctgttt ttggttgaac ttttgcatat tttggatatt
     6361 tagggaattt agctacacaa cggaaagccg tcatccctgc atatttttcg taatatttct
     6421 tcgttggccc ttcaaaatcc ttgctccacg gtttcacatg tcctgaaaaa tggataatgt
     6481 aaggattgtt gcgagtttct tcgtattcac gttcacctgc tgctgttgga tgtttttttg
     6541 cttttgccat gatatagcct tgtgcattcc aacgcggatg aagtggtaac caacggtcat
     6601 gtaagatagc atttaatgca tcttggtcat ggaatcgtaa tttttctgga ttatgttcga
     6661 tgaaatctaa tactttttgc gtaatatttt catctaacca ttttttcaca ttgatcagca
     6721 tcaaacctga gttgaaataa cgcattgatt tagctgaaat tttcattttt tccagtcgtt
     6781 gatggaaccc agcgtcttca acagctgcta cgattgaatc accgaagtca agacgccata
     6841 atttagagat atcttgtaaa gcgatcatgt cgcaatccat atataagata cgttcaactt
     6901 ctgtgccgcg gaacaattcc ggaatcgcaa tacgataata agctgtttct ggaatacgat
     6961 cgctgattac cacatttgta aagaattctt tattgatttt caagaattcc acatctgagt
     7021 tcattcgtgc gtttttcact gagaagcgta aaagctgttt actttcaaaa tcgatatcat
     7081 cgtcaatcac atagaaccga acaaatttag acgggttgca attatctaat actgagacaa
     7141 ataatgcagc taaatgtggt acgaattttg tgttacaact tgcgactact gctatttctt
     7201 ttttattcat tgatctcgcc tactttcgtt agagtactgt gtgctaattt ttttaagtat
     7261 aaattggtat acggatgatc tttcaacgta ttccaaggtt tatcatgtcc tgtaaagtga
     7321 acgatcgaag gtttttcatt tccttcttta tactgacgtt catatttttc atttggtgct
     7381 ggatgacgtt caaaaatcag cgaagtctgc atgttccatt ttggatgaag ttgttcccaa
     7441 tcttcataaa gtacagcatt caaagcatct tggtcgtgat agatgattcg atcgccattt
     7501 tcactgaggt aatggatagt tttttctgta atttcttttt tattccattg atcaatgtca
     7561 atcaccatca ctcctgaatt gaagtacaag tcatcggaat cgattcctaa tctttccaaa
     7621 gctttcactt ggcctggatc gataatagcg ccgattgttt ttccatttaa cggctcatca
     7681 tatagtttta cgatatcgtc tagtactaac acatctgaat ctaagtaaag gactttctta
     7741 taatcctctt tagctaataa gtttggtaaa gaaattctta agtaagcggt agttgtgata
     7801 tggtcactga ctaggaagtc ttcgtagata tctttttcca ctgtcaggaa ttggatactt
     7861 gcattacttg aatatttatt taccgtttct tccaatcctt ctttactata ttctgataga
     7921 ccatcgtcaa tcacataaaa ttttatgcgt cttgttttat tgcaattttc taatgcagtt
     7981 gcgatcatca cgctcaagta aggagcataa ttttcgtctg aagcagttac cacaggaact
     8041 acgccgtatc tcttctccat caaaatcact cctttttcta atctttttct aacctgttct
     8101 acatctagaa tattcctcat ttggttttaa agcaaaatat atgctttgtt ttcattgaat
     8161 attttaaaat ttttttatca caaaacgcat cggtaagcgc taactattcg tttttcatgg
     8221 ctttttattc aaaggtattg accaaaaact actatatata gccacctttt tcttaagaat
     8281 gttttttgaa aaaattcata atgacaaagc aagttagttt cattttttta aattctcatt
     8341 taatcttaag gtaacaggaa aatagatatg taaaaagaaa gtaggaagat aaaatgaaac
     8401 tagcagcgat tgatttagac ggtaccttgc ttgatcgaaa tggaaagata ccagaagaaa
     8461 atatccaagc acttcggaaa tttgatgaaa atggcggaat cgtaaccgtc gctaccggaa
     8521 ggaacagcat ttctgctaaa gatatttttt ccgaattagg aatcggtggc tatttgattt
     8581 cttccaatgg ttcactgatt gctgaaatga aggatggaaa aatggcgaaa gttctcagac
     8641 gttcaaaaat agatagcgat acactgagaa aagccttctt ctatgctcaa gaagcgaaaa
     8701 tatccatcat tgctagccga gaaacccaag atgatcagct gacattcgat gaatcaaatc
     8761 tagtcaaaga tgacccttat tatgaacatt tcaatttaca gaaccattct tttgatcaaa
     8821 ttttaaatat gttggaagat ccttctctta gctatcttaa attagcatta acagataaga
     8881 gtgaagaaaa actacagaag ctacaaagag atctgcaagc agatggtatc caatcagtat
     8941 tctctgatcc gcatttctta gaaatcacgc ctacaggtat cactaaagct cattcacttg
     9001 tttttctggc ggattacctt ggaatcgaat cgagtgacat catggctttt ggcgatcaag
     9061 aaaacgacat tgctatgttg aattacagcg ggttgagtat tgcgatgggg aatgcacagc
     9121 gacaagtaaa agaaattgca aaccaagtaa cactgacgaa tgaagaagct ggtgttgctt
     9181 atattttaaa aaacttctat taaaaagaat aaaatgacga tatgaatgta ggaggaaaga
     9241 aaatgttagt tattattgat atgcaaaatc atattcttga tccaaacagc gaattttata
     9301 ttccagagtc agatcaattg gcagaacgta ttgctcaaag attagctaaa gcacgccaaa
     9361 ataatgaata tattctcttc acacgggata taccagttga actgaaggac aaagaagaag
     9421 aacgagaaga tttgcaattg atccctcttc tttctccaca agaaaatgaa cgagtgatta
     9481 aaaagtatta ctttacgatt ccacctgaaa ctttagctga aatccaggaa actttatttg
     9541 aaagcaaaga agaacaaaaa gagatcgaag tagtcgggat tgaaacaaac ttgtgtgtac
     9601 tctctaatct gataggcctg cagagtgctt ttcctgaagc agactttttt gttgatccaa
     9661 tgttagtctc cagtagaaaa cacggagaat ccgctttaga gctgttgaaa gatttcaacg
     9721 tctctatcgt cgaatcataa aaatatctat cttcatttac gatatttaac acacaataaa
     9781 aaaagcacgc agaactgttt tagtcctacg tgctttttct atcaaagtag attggctttt
     9841 ttatgacaca gagtgaaacg gcaggctcac gacctctttc ttaaatggtg ttcaatcagc
     9901 tgaccctcgg tattagctta acaaacggca aaatatgagg aactattttt ccgtttgtta
     9961 agctaatact caggtcaaaa cgctgatttc acaacctctt ccttaaacgg tattcaaaag
    10021 cttgcatctg cagtaataag aacagccaag taaaaacata agaagctgtt tttacttggc
    10081 tgttcttatt acttgatgca gggcagcttt ttcacaacct cttatgtacc ttgtaggact
    10141 cgaacctacg accggacggt tatgagccgt ctgctctaac caactgagct aaaggtacat
    10201 cataaataaa atttattttt gttacttttc aactatagca cgaatgacaa actctcacaa
    10261 gtttcaaaat ataagtaaaa taatattgtt attcttattt cttttacttg tgtcataact
    10321 attttgttac tttaattttt atttgcttat tttagaaaaa atgttattct tttatttatt
    10381 ctcaatgtta ggagtgatta ttttgccatt tattgcgatt ttattcgatt tagttgcggc
    10441 agcagcttat tttctccaat taaattatca aacctcagga attctgttta ttggaatgat
    10501 ttttcaaggt gtgattaccc ttttgcttct tgtcatgatg ctcagctata aaggaaaaag
    10561 atatgcccgt attcaaacaa aagtatttgt taaatatgtt tccattcgct atgggatcat
    10621 cattctttcc tttttagtga atgcgatcgt cctcttttta tatgtattga attacttaga
    10681 tatcaatcca ttgatttttt ctcgatagat aagacacatt cagcggaaca gttcctgctt
    10741 atcagcagaa aactgttctg ctttttcatt tttcattgat ctattcttct tttattttcc
    10801 tagcatttca tttgtgatgt atgcaataac atgttatctt ttattatatg aatcgttgag
    10861 gagtccgtta catgattacg ttggtgagag ttttgttttg ggtgcctgcg gtcgcactgg
    10921 tcgcaagtat tgtttatctt atgaattgga acaaagaacg gttttattta gctattttga
    10981 ctcttccggc tatctacttt atgtggaaag tttttaatta caactacttt gaacccgata
    11041 gtgtttttat agaagaatta tcaggactgg ttttgtcttt attgatcgtc attctttatt
    11101 tgatacgttt aaataaaaaa cattgagtac tattgattgc atgtgtagct ttctatagtc
    11161 attataacta ttctactata atgaaaggtg attaaatgag tatcgaagaa gctattttac
    11221 tggatacttt acgttgtcgt ctgaatttct taagaactgg ccgatcttta tctcaagtag
    11281 ctgtttctag cgcgttaagt attcctttat tcaaatatcg taagtacgaa gaaggagaag
    11341 aaattccaag catcattgaa ttaatctata tcgctgactt ttacgatatt tccgttgatt
    11401 acctaattgg tcgctcagaa acagcaagca aactgaagtg attttttcca gccttggtgc
    11461 tggttttttc atttaacgtc taaggttaac aatctatata taaaggagac atttctatgc
    11521 gattatggca tgaaaaattg atttcaaagc ttcctcgtca acagctttta ggacaacatc
    11581 gggaatgctg tgcattaaga ggaaatggct ggggaaagaa acatgctact gtggattatg
    11641 tttttcacta ttctccttat agattattcc aatatcatca gcttgtcatg gatgaaatgg
    11701 aaaaacgtgg ttacagccca gcaccagagt ggaaaaatcc tatgtatcgt ggcaaatcat
    11761 gcgaagcatt ttttgattta cctactgtag agcgcaacgt gccgatttat ccggaacacg
    11821 atgaagttta tctagcagaa tgtttagcaa atttggagca aaaaggaatt tatcttacct
    11881 aatataacta gaaaagcctt ctatctcctc ttacttcatt aaaaagcttt aaccatctta
    11941 gatcaagcta aaatgtcgta agttttaggg ttatcttcat cctcacgtgc aaaactaaaa
    12001 gcgtactcta gttttggttc atcgccaact tccctagaca taaatgaaca tctcctgacg
    12061 tggtttaaaa gtaccgcatc ttcgacgtct gtatacgatg cacagcacag attgcttctt
    12121 ttgatctttc ttttgatttt tctgttgctt cttctacacc ttcttcgatt actacatcga
    12181 tgcttacaat tgcttctgcc taatattccc atgcatcgtc gatttaagcc tctgtagtca
    12241 ctgtgatcgt ttccacattt taactcctct gtcgtggctt cagttgattt tgtgatcagt
    12301 tcagttgcta agccagagtc agaacaacct gccattgcta gcgttaatat tgatagtaaa
    12361 aatagttttt ttatcataag gttccccccc tgaaaataga ctaaaataat aacgctttct
    12421 ttttttgttt tcagacgaaa attaataatt tctaaatatt ctttaaaaaa gcttataact
    12481 attatgcctt ttttaatata taacac
//
LOCUS       DANJLF010000072        12119 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000072 DANJLF010000000
VERSION     DANJLF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(31..588)
                     /gene="frr"
                     /locus_tag="PWT25_002239"
     CDS             complement(31..588)
                     /gene="frr"
                     /locus_tag="PWT25_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL1022287.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(591..1313)
                     /locus_tag="PWT25_002240"
     CDS             complement(591..1313)
                     /locus_tag="PWT25_002240"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL1022288.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1449..2330)
                     /locus_tag="PWT25_002241"
     CDS             complement(1449..2330)
                     /locus_tag="PWT25_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL1022289.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2429..3211)
                     /gene="rpsB"
                     /locus_tag="PWT25_002242"
     CDS             complement(2429..3211)
                     /gene="rpsB"
                     /locus_tag="PWT25_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL1022290.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3570..4049
                     /locus_tag="PWT25_002243"
     CDS             3570..4049
                     /locus_tag="PWT25_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL1022291.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4266..5357
                     /gene="phnW"
                     /locus_tag="PWT25_002244"
     CDS             4266..5357
                     /gene="phnW"
                     /locus_tag="PWT25_002244"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL1022292.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5350..5478
                     /locus_tag="PWT25_002245"
     CDS             5350..5478
                     /locus_tag="PWT25_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL1022293.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5482..6027
                     /locus_tag="PWT25_002246"
     CDS             5482..6027
                     /locus_tag="PWT25_002246"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL1022294.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6481..8172)
                     /locus_tag="PWT25_002247"
     CDS             complement(6481..8172)
                     /locus_tag="PWT25_002247"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL1022295.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8592..9539)
                     /gene="arcC"
                     /locus_tag="PWT25_002248"
     CDS             complement(8592..9539)
                     /gene="arcC"
                     /locus_tag="PWT25_002248"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL1022296.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9654..10673)
                     /gene="argF"
                     /locus_tag="PWT25_002249"
     CDS             complement(9654..10673)
                     /gene="argF"
                     /locus_tag="PWT25_002249"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL1022297.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10764..11993)
                     /gene="arcA"
                     /locus_tag="PWT25_002250"
     CDS             complement(10764..11993)
                     /gene="arcA"
                     /locus_tag="PWT25_002250"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL1022298.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3309 a         2522 c         2193 g         4095 t
ORIGIN      
        1 atgatccgcc cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc
       61 agcagcgatc ttatctactt ctttgatgct gttatctgtc aatgtttgta cgtctttttc
      121 taatccgcgc aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc
      181 gatcgcatca cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttaac
      241 atctttagct aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac
      301 atttccatca ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc
      361 ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat
      421 tgatgccatt tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa
      481 aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc
      541 tgcttttttc attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc
      601 cttacagttg ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt
      661 aaattgaaga caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc
      721 atgacttgta atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca
      781 tttgcatcta ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg
      841 ataacatctg cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac
      901 gggttgccgg taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct
      961 ttacggcgaa tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc
     1021 ggtacaccca cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac
     1081 atacccatgt aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc
     1141 cagatatttc cgccgcccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg
     1201 atttcttgga tgatttcttt gattactggt ggtttgattc caaatcctgc gtccccagct
     1261 aaagcttctc cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt
     1321 cctccaatat tttctattgc cattttacca tacttagatg atttcgacac tctaagactg
     1381 gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt
     1441 caacagaatt attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt
     1501 tcgatacctt cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca
     1561 acgtattttt caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt
     1621 tctgctttga atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg
     1681 ccttcgttca atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga
     1741 atttgtgatt cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt
     1801 gcaactgttt cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata
     1861 tgcaagtatc caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag
     1921 ctgatttttt ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta
     1981 tctgttttga ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct
     2041 gcaatttctt ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag
     2101 tttacttcaa cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct
     2161 gcaatgcggt catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact
     2221 gctttttcca tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg
     2281 ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc
     2341 tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca
     2401 gcctttgaaa cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa
     2461 catctacgat ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta
     2521 cttgatcttc accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt
     2581 taacggcacg aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc
     2641 agtttgtatc aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa
     2701 tacgttcttt acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga
     2761 taccgcctaa gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt
     2821 taggtagaac ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga
     2881 tacgtttttg gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga
     2941 agtattgtcc tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac
     3001 ctacgaataa tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat
     3061 ctactaattt aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga
     3121 tatatttctt catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt
     3181 ctagtaattg tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt
     3241 tcctcttctt atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat
     3301 aagaatgtgg atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg
     3361 tatcgcaaga gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa
     3421 agaattcaag aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat
     3481 aattttaggt ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca
     3541 tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta
     3601 aacaagtcat tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc
     3661 attatggcgt taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg
     3721 tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc
     3781 ctgagactag gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga
     3841 aagtcgaacg agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat
     3901 ttgcatctgt tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg
     3961 atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc
     4021 accatccaac tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt
     4081 gtttttttat tttcaatatt ttccttattg aataaacctt tacactcttt acaaaaatac
     4141 atcatttttt attcaaattt ttcaacacct tgattgaatc acaataatca tcatttatat
     4201 tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa
     4261 atcatatgta taaattatta acttctggac cattgacgac ttctgaaaca gtgaaaaatg
     4321 ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa
     4381 gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc
     4441 aaggtagcag ctccttcggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata
     4501 ctgtactgat ttgttcaaac ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc
     4561 tgaaactgaa ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga
     4621 ttacacagct gattgaagag aatccgtcta tctccgcact agcaatggtc catagcgaga
     4681 ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa gaaagagatc
     4741 ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgatttgt gtagccgaac
     4801 tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt
     4861 cttttgtact tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt
     4921 cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca
     4981 cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag
     5041 gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa
     5101 tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt
     5161 ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa
     5221 atggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata
     5281 ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg
     5341 agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc
     5401 tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact
     5461 gacaaagaaa tccgctaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt
     5521 tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa
     5581 gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat
     5641 tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat
     5701 attggttcca cgaatggatt taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa
     5761 aaagaaaggt attcaccaga cttcatggca acttttgaag acgtcgaagg gtatggtcga
     5821 ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa
     5881 gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg
     5941 acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa
     6001 ttagagatgg ggaaaaagat cgtttaaaac agtctgttgc tcttagattc caacaagccg
     6061 gcgctgatat ttgtgtaaac aatttaaaag agttaacaga atacattttg tacgctgaca
     6121 tgaatccaga atgactttta tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag
     6181 taataaaaac agccaagcaa aaatagctcc tcatattttt cgtttggtta ggcttattac
     6241 cgcagatgca agcttttgaa caccgtttat tcgaaaaaaa aaagggagct gtgacacgac
     6301 ttttgtcaca gtccccttat ttgcaaacaa acagtgttta agagacaacc tctccactaa
     6361 acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct
     6421 attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc
     6481 ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt
     6541 cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt
     6601 atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga
     6661 cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac
     6721 cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc
     6781 tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc
     6841 tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt
     6901 caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc
     6961 caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac
     7021 ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat
     7081 caaaccaaat ggaatatggt gcatgtcgtc agaccagtca aagcccattt ccttcaagac
     7141 agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt
     7201 gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt
     7261 cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat
     7321 ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc
     7381 catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc
     7441 atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc
     7501 ctctttatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg
     7561 ttctgtttca gctacttcat ggaattggac gtataaacga agcaattcat tgattggttc
     7621 tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg
     7681 tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat
     7741 gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc
     7801 aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatgt tgctatcccc
     7861 ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt
     7921 catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat
     7981 tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga
     8041 aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgctc
     8101 gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc
     8161 tttattattc atctttattc ctcctaaagt gttcccttga ttgttaaaca aaaaaaatct
     8221 ctagcgacgg catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc
     8281 aattgtccta aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac
     8341 agaattctcc aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt
     8401 cgctagccaa gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt
     8461 tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt ttcataaaaa aacaatccag
     8521 atggcctaaa ggagaagaaa taccacctgg atagctataa atcatttcgt aaatacgaaa
     8581 gacttaacaa attaatcagc tacgataatc gttccttctt cagaagctaa aagattctcg
     8641 atattttcta aagaagtgat gattgcctta gcgtttggtt ttgcttcaac aaatgcaata
     8701 gctgcttcta ctttcggaag catgcttcct ggagcgaatt gcttttcatc aatgtattgt
     8761 ttcatttctg aaacagtaac agtttctaat ttcttttgat ctggtttttg gtaattgaca
     8821 tacacattgt ctactcctgt caatacgatc agcaaatccg catcgatgat ttcggctaat
     8881 ttttctgaag caaaatcttt atcgattacg gcttctcgtc cttctaatcc agtagctgtt
     8941 tctacaacag gaatcccacc gccaccaaca gagactgtga taactccttg atcgaccagc
     9001 gtttcgatga cacgtgcttc tttaatcgag atgggtttag gggaagcgac tacttttctc
     9061 catccacgtc cagcatcttc tttgaatgtc acagtggagt cagcattcat ttgttctttg
     9121 gcttcttctt ccgtgtagaa tggaccgact ggtttacttg gatttttgaa tgaaggatca
     9181 ttttcatcca caataacttg agtgactaaa gaaaccacat ctttatcgat ccctttttcc
     9241 ttcagtactt cgcccatggc gttttgtaac cagtaaccga tagatccttc tgtcattgca
     9301 acacatgtat ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc
     9361 aataggttac cgacttgcgg accatttccg tgggagatga tcaattcatc tccttgttcg
     9421 ataaatttca ctaaatactt tgctgtttcc attaacgctt cttgttgggc ttttgcactt
     9481 gcatccgtag acaagatggc attgccccct aaagcgacaa ctacttttcg tttggccatt
     9541 tcattcgtcc tttcttcagt tctaccgaat tttctagata acaaaacgag ccagcgtttt
     9601 gtgattttcc ctcaatgctg actcgttttt tcgtgagatg aatgtggtta tgcttaaacg
     9661 cgagggataa acaagttacc taatgttgct gccatgatcg ctttgattga gtgcatacga
     9721 ttttctgctt ggtcaaattg acgagcatat ttgctgcgga agacttcatc tgttacttcc
     9781 atttcagtga tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg
     9841 aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt
     9901 tccatattga tttggtaagg tttcaacaat ttgatacgtt cttcgaattt atcttcttca
     9961 cccattgata cccatacatc agaataaagg acgtctgctc cgtctacacc tttatcaaca
    10021 tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa agccttctgc cattttgacg
    10081 atttcttctt caggttgtaa ttcttttggt gcaacgatac gcatattcgc tcctaaaatt
    10141 gcacctgtga ctaataaaga gttagccata ttgtttcgac catctccgca gtaagcaaca
    10201 gtgatacctt ctactgttcc aaagttttct tggatagtta aaaagtcagc gatcatttgt
    10261 gttggatgcc attcatcagt caagccattc caaactggaa caccagagaa ttctgctagt
    10321 tcctctacca ttttttgact gaatccacgg aattcgattc cgtcaaacat acgtcctagt
    10381 actttagctg tatcttctgt ggattctttt ttacctaatt ggatatcatt tgcacctaga
    10441 tattcaggat gtgcacctag atcgatcgct gcagtagtaa atgcagaacg agtacgtgta
    10501 gatgtttttt caaataaaag cgcaatattt tttccttcta gatagtgatg tgggatacca
    10561 cgttttttca agtctttcaa gtgttctgaa aaatcgatca aatattgtaa ttcttctttt
    10621 gtaaagtctt tttctgctaa taagcttctt ccttggaata ctgattcttt catattaaac
    10681 attctccttt agattgtttt tatttaagat taaagaggaa atcccgacgg cagctgacag
    10741 taagactgcc gtcaggaaac aaattacttc aagtcttcac ggacaagtgg ttggctcatg
    10801 cagcgtggac caccacgacc tcgtgacaat tcacttgaat tgatttctaa cactttgatg
    10861 ccatagctac gaagcaattc gttcgataca taatttcggt tataagtgac tacaacacct
    10921 ggagcgattg ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc aacgatctca
    10981 tctccattac cagttgggat cagtacaaga tcatctaatc caagagcagc tttcatcgtt
    11041 tcgtgtaagt tatctgaatg agtaatcttg atatcatcac catctggttc gattgtaaag
    11101 acatccatct taccgtcttt actttgaatt gctggatgga tagtgaattt atcgtgatct
    11161 accattgtaa atacggtatc caaatgcatc attgcacggt tgttcggaat tttgattgcc
    11221 aacacttttt caaatcctga attttttgcg aataggtggc gtgctaattt ttctaaagct
    11281 ttcgcattag ttcgttgaga aatcccaaca gctactactt tatcgcttaa gatcaattcg
    11341 tctccgcctt cgatatgatc tggattttca cgatccaacc atacttcgat tcctttattt
    11401 gcaaatcttg gatgatgttt caaaatatat tctgtaaaca tcgattcacg acgacgtgct
    11461 tcaaaagtca tcttattgac tgtcatccca tttcccattg atgcggaagg gtcgcgtgta
    11521 aagtacaagt ttggcattgg gtccatgtaa aatggatagt catcgtctgc tgacagatca
    11581 tatagactgc tagaacgtac ttcgatatct tttgtacgaa ccccagccat gattttatct
    11641 accatttctt gcgtttccat tgatagaagg aactcatgaa gaccctctct tacagcattc
    11701 gatgcaatat gagattcatc caacatttta ttcaagaatt gttctttgac attgcctgcg
    11761 tcaatcgctt ctgccgctaa tttttccaag taaagagtct cgacgccttc attttgcaat
    11821 gtcttcgcaa agttgtcatg ttcttcttga gcaatcggaa gatatggaat atcatcgaac
    11881 aacagacgat ccataatatc tggtgtcaaa ttttccactt cttgtccagg ccgtttcagt
    11941 aaaactgtct ttaatttccc tatttcagag aaaacgtgaa taggtttatc catgttgtgt
    12001 tgcctccttc tttttgatta catgtatagg atatcctcct tttgtaatca ctttcataat
    12061 tttgtctcat tcaaaatgag acattcctca cattaatatt cgataattaa ataattaaa
//
LOCUS       DANJLF010000073        11667 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000073 DANJLF010000000
VERSION     DANJLF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(37..216)
                     /locus_tag="PWT25_002251"
     CDS             complement(37..216)
                     /locus_tag="PWT25_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022299.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="PWT25_002252"
     CDS             complement(597..1304)
                     /locus_tag="PWT25_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022300.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="PWT25_002253"
     CDS             complement(1297..1734)
                     /locus_tag="PWT25_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022301.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="PWT25_002254"
     CDS             complement(1749..2000)
                     /locus_tag="PWT25_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022302.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="PWT25_002255"
     CDS             complement(2000..2206)
                     /locus_tag="PWT25_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL1022303.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="PWT25_002256"
     CDS             complement(2643..2903)
                     /locus_tag="PWT25_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022304.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="PWT25_002257"
     CDS             2978..3181
                     /locus_tag="PWT25_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDL1022305.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="PWT25_002258"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="PWT25_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1022306.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="PWT25_002259"
     CDS             complement(3810..4709)
                     /locus_tag="PWT25_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL1022307.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="PWT25_002260"
     CDS             complement(4722..5315)
                     /locus_tag="PWT25_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL1022308.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="PWT25_002261"
     CDS             complement(5391..5540)
                     /locus_tag="PWT25_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022309.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="PWT25_002262"
     CDS             complement(5556..7760)
                     /locus_tag="PWT25_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL1022310.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="PWT25_002263"
     CDS             complement(7771..9231)
                     /locus_tag="PWT25_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL1022311.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="PWT25_002264"
     CDS             complement(9292..11664)
                     /locus_tag="PWT25_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022312.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DANJLF010000074        11508 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000074 DANJLF010000000
VERSION     DANJLF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(110..655)
                     /locus_tag="PWT25_002265"
     CDS             complement(110..655)
                     /locus_tag="PWT25_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1022313.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(667..1359)
                     /locus_tag="PWT25_002266"
     CDS             complement(667..1359)
                     /locus_tag="PWT25_002266"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL1022314.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1378..1662)
                     /locus_tag="PWT25_002267"
     CDS             complement(1378..1662)
                     /locus_tag="PWT25_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022315.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1675..2238)
                     /locus_tag="PWT25_002268"
     CDS             complement(1675..2238)
                     /locus_tag="PWT25_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL1022316.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2466..3128
                     /locus_tag="PWT25_002269"
     CDS             2466..3128
                     /locus_tag="PWT25_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL1022317.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3252..4448
                     /locus_tag="PWT25_002270"
     CDS             3252..4448
                     /locus_tag="PWT25_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL1022318.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4549..5925)
                     /locus_tag="PWT25_002271"
     CDS             complement(4549..5925)
                     /locus_tag="PWT25_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1022319.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5962..6828)
                     /locus_tag="PWT25_002272"
     CDS             complement(5962..6828)
                     /locus_tag="PWT25_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL1022320.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6917..7942)
                     /locus_tag="PWT25_002273"
     CDS             complement(6917..7942)
                     /locus_tag="PWT25_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1022321.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7960..8634)
                     /locus_tag="PWT25_002274"
     CDS             complement(7960..8634)
                     /locus_tag="PWT25_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1022322.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8818..9711)
                     /locus_tag="PWT25_002275"
     CDS             complement(8818..9711)
                     /locus_tag="PWT25_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1022323.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9785..11482)
                     /locus_tag="PWT25_002276"
     CDS             complement(9785..11482)
                     /locus_tag="PWT25_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL1022324.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3412 a         2522 c         1973 g         3601 t
ORIGIN      
        1 taagtttttt taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct
       61 cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt
      121 ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac
      181 ttgccacggc atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg
      241 tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt
      301 ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc
      361 aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt
      421 cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg
      481 aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact
      541 tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct
      601 ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag
      661 tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc
      721 gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc
      781 acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc
      841 catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga
      901 gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt
      961 tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc
     1021 tggtaattgt cctggtttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt
     1081 gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt
     1141 attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa
     1201 gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat
     1261 aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt
     1321 tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta
     1381 tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt
     1441 catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat
     1501 tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg
     1561 ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg
     1621 cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt
     1681 tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa
     1741 ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc
     1801 gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg
     1861 aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt
     1921 cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc
     1981 ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg
     2041 aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccacc
     2101 aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac
     2161 agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa
     2221 ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc
     2281 atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc
     2341 tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa
     2401 taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt
     2461 ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg
     2521 tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt
     2581 tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga
     2641 ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt
     2701 ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc
     2761 agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt
     2821 tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata
     2881 cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga
     2941 tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt
     3001 cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg
     3061 tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa
     3121 aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt
     3181 tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg
     3241 aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata
     3301 cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg accacttcac
     3361 aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac
     3421 taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg
     3481 cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat
     3541 cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac
     3601 tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct
     3661 tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa
     3721 agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc
     3781 taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac
     3841 tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc
     3901 cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg
     3961 attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac
     4021 agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg
     4081 ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga
     4141 tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa
     4201 caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag
     4261 cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt
     4321 tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc
     4381 atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa
     4441 cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac
     4501 tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt
     4561 catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat
     4621 accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat
     4681 ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc
     4741 aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat
     4801 gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc
     4861 cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat
     4921 aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat
     4981 catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac
     5041 tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt
     5101 atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag
     5161 tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc
     5221 cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac
     5281 accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc
     5341 tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg
     5401 taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc
     5461 aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag
     5521 ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga
     5581 ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc
     5641 accaaataca acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa
     5701 ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga
     5761 cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc
     5821 gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc
     5881 actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca
     5941 ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa
     6001 aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc
     6061 aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc
     6121 aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa
     6181 taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa
     6241 gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa
     6301 attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc
     6361 tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc
     6421 ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac
     6481 ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat
     6541 taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg
     6601 gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat
     6661 ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc
     6721 gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc
     6781 aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct
     6841 tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt
     6901 cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag
     6961 tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg
     7021 ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat
     7081 ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc
     7141 ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt
     7201 ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga
     7261 tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt
     7321 tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat
     7381 attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt
     7441 ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa
     7501 tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat
     7561 ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta
     7621 cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg
     7681 tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc
     7741 cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct
     7801 caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca
     7861 tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt
     7921 tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg
     7981 tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg
     8041 attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg
     8101 cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg
     8161 atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac
     8221 attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga
     8281 acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac
     8341 aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga
     8401 gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc
     8461 caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga
     8521 gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc
     8581 catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa
     8641 tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc
     8701 ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc
     8761 gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta
     8821 tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa
     8881 aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt
     8941 ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac
     9001 tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg
     9061 cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac
     9121 ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc
     9181 ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg
     9241 tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa
     9301 caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat
     9361 ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc
     9421 ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt
     9481 ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc
     9541 aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc
     9601 taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg
     9661 taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac
     9721 cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg
     9781 ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca
     9841 aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc
     9901 cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt
     9961 cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa
    10021 gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt
    10081 gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc
    10141 actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata
    10201 ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac
    10261 tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct
    10321 ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt
    10381 tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat
    10441 ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt
    10501 ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt
    10561 ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa
    10621 ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag
    10681 aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta
    10741 gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa
    10801 ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca
    10861 aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca
    10921 tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag
    10981 ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg
    11041 tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac
    11101 ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg
    11161 cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat
    11221 cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac
    11281 caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt
    11341 ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttggcta
    11401 gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg
    11461 tgacaatcat ttttttattc atttttttct ccccgttctt tttttatt
//
LOCUS       DANJLF010000075        11323 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000075 DANJLF010000000
VERSION     DANJLF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            285..1544
                     /gene="lysA"
                     /locus_tag="PWT25_002277"
     CDS             285..1544
                     /gene="lysA"
                     /locus_tag="PWT25_002277"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL1022325.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1531..2331
                     /gene="dapF"
                     /locus_tag="PWT25_002278"
     CDS             1531..2331
                     /gene="dapF"
                     /locus_tag="PWT25_002278"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL1022326.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2334..3215
                     /locus_tag="PWT25_002279"
     CDS             2334..3215
                     /locus_tag="PWT25_002279"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL1022327.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3212..3928
                     /locus_tag="PWT25_002280"
     CDS             3212..3928
                     /locus_tag="PWT25_002280"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL1022328.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3934..4629
                     /gene="dapD"
                     /locus_tag="PWT25_002281"
     CDS             3934..4629
                     /gene="dapD"
                     /locus_tag="PWT25_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL1022329.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4645..5646
                     /locus_tag="PWT25_002282"
     CDS             4645..5646
                     /locus_tag="PWT25_002282"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL1022330.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5643..7004
                     /locus_tag="PWT25_002283"
     CDS             5643..7004
                     /locus_tag="PWT25_002283"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL1022331.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6964..8115
                     /locus_tag="PWT25_002284"
     CDS             6964..8115
                     /locus_tag="PWT25_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL1022332.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8090..9259
                     /locus_tag="PWT25_002285"
     CDS             8090..9259
                     /locus_tag="PWT25_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1022333.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9434..10813
                     /locus_tag="PWT25_002286"
     CDS             9434..10813
                     /locus_tag="PWT25_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1022334.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11094..11323
                     /locus_tag="PWT25_002287"
                     /pseudo
     CDS             11094..11323
                     /locus_tag="PWT25_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3773 a         1967 c         2516 g         3067 t
ORIGIN      
        1 gattcataaa attgaaaata tccgaactat tccgaactat attgaagcga aaatattttt
       61 tcatcactaa atagtgttga atttatgttt ttatgtataa atagcttcta atagttcggg
      121 tattttgttt ttataaccat ttttattctt atttttaaaa aaatatttcg aaatatacta
      181 gacaattcag aaaattttgt ttaatattat tccaataaaa ttcaaatctt tatcaaatga
      241 aattccaata aaaacggaaa aaaagtgaaa agaggaatga aaaaatgaca gaattgataa
      301 ttggcggagt cacagcaagc acattggcag aaaagtataa gacacctctg tatgtgtatg
      361 atgaagaaaa gatgagacaa acgatgctgt cattcaagcg aggctttgta tcgaaacgtt
      421 ttgatacaaa agtgctttac gcctctaaag cattccaaac gattgagatg ctgcatttag
      481 ttcgtgaata tggattcggg ctagatgttg tcagtggtgg ggaaatctat accgctttgc
      541 aagctgattt tcctagagat actatctact tccatggtaa caacaagacg ccagaggaac
      601 tgatctatgc acttgaaaat gatttgttgc atattgttgc cgataatttg atggaagttg
      661 aattattagc agaattaagc caacgctatt taaaaaaaat ctgtatcatg cttcggttga
      721 atgtcggagt agaagcacat actcatgaat atatcgtcac tgcgcatatt gattcaaaat
      781 tcggcatgag ctatgaaagt aaagagtgcc aacaagccct tcagttgata aaagaaagca
      841 gctatctaga gttagaagga tttcatgctc atatcgggtc acaaattttt gatgttactg
      901 cttggttagc tgaaatcgac aaattgacga attacttaac cgattttgac caacctcttt
      961 ccttgaatct tggcggaggg tttgggattc gttatacaca aaatgatcag ccgatcccag
     1021 tcgaagaggc actaaaaaaa ctgattgaat atacggaaga agctttggaa aaacgaaaac
     1081 tgaccattcg tcaattactg atcgaaccgg gtagaagtat tgttggagaa gctggtacaa
     1141 cactatacac gattggtttc atcaaaaaga caccacataa aaaatattac tttgtagatg
     1201 gcgggatgac agataatatc cgtccagctc tgtatcaggc agaatacgat tgtgatttag
     1261 cagcaaaact gtcggaagaa aaaacagaga aagtgacgat tgcaggaaaa atgtgtgaat
     1321 caggcgatgt ggtaattaaa gaagcttatt tgccgaaagc agaatcgggt gatcttttag
     1381 ctgtttattc cacgggagct tatggatact cgatgagcag caactacaat cgagcgacac
     1441 gaccagcagt tgtttttgtc aataatggcc aatcaagact gattgttcgc agacaagcat
     1501 tttctgatct actaaggggg gaagtttcct atgatccttc ataaatataa tggctgcgga
     1561 aatgatttta tcttattaga ttacgcagaa gacacagatt atagtcaatt agccgcttct
     1621 ttatgcacaa aagaaaaata tgatacagac ggattgatcg ccgtaaaaat cgatccgttg
     1681 gaaatgatct attacaacaa agatggcagc cgcgcgccga tgtgcggaaa tggcattcgc
     1741 tgttttgctc gatacgtaaa tgatcagaac atgatgaatg aacaggaatt caacgtagag
     1801 acattagcag gcacaagaaa aattacggtg ttggatccct caccttttta ttgcagcgta
     1861 gatatgggag tgccagatga ttctccggaa aaaatcaaag ctaatcaaac ttatccaata
     1921 aaaaaccagg aaatagagat caatggtgaa aaagtaacga tcactagttt atttttagga
     1981 acgattcata cagttgtttt tgttgaaaat gcgcgtgagg aagtctggag aaaaagaggt
     2041 gaactgatct gtcatcatcc tttgttcaaa gaaaaaacca atgtcaactt tgtagaagta
     2101 gtcaatgaac aagaattgat cgtccgtacc tatgaaagag gagtaggttg gacacttgct
     2161 tgcggtacag gttgctgtgc ctcttatgtg gttgctaagg aaactgggaa aatttcagca
     2221 gatcaggtag ttgttcactt ggaacaagga gacttgatga tctctggtga ggatgcgata
     2281 caaatggctg gcccagcagt tcatgaatgg acaagaatat tggaggaatg aagatgataa
     2341 aaggttcgat cgtggcgctg atcacgccga tgaatgaaga gggatcagta gattatgctg
     2401 gtttggaaaa attgatccaa ttccatttag atgaacaaac agatggatta ttagttttag
     2461 gtacaacagg agaatcttct acgctcacac aatctgagga ggagcagatc cttcaactga
     2521 cagtaaagaa agtggctggc cgtgtgccgg tgattgcagg agctggaacg aataatacga
     2581 aagaaacgat tgaaaaagcg aagcattttg cttctttggg ggcagacgca cttttggtca
     2641 tcacccctta ctacaacaaa acaagtgatg caggactagc tgctcatttt acagcaattg
     2701 cagaagcctc accgattccg cttattttgt ataatgttcc ttctagaaca ggcatgtcga
     2761 ttccaatcca tgtattagta aatttagcag aacatcctaa tatcattggt ttgaaagaag
     2821 cctcaggaga tatggcttat gtgatggatg ccgctcggtt gattggagaa gaattcttcc
     2881 tttacagtgg aaatgatgat ttgatcctac cagtaatgag tgtaggagga agcggtgtaa
     2941 taagtgtatg ggcgaatatc caacctaaaa tagtccatga attagtaaaa gatacgcagg
     3001 acggaagatg gcagcaagca aaagaaaagc aattaaatgc acttgaactg atccatgcct
     3061 tgttcagtga gaccaatcct attcctgtca aagcagccat gtcattatta gacttacctt
     3121 ccggacctct acgcctgcct ttagtttctc tttcaaaaga gaaaaagaaa caattagctc
     3181 agcttttgtt aaaagaaagg acggttgaaa aatgaaaatc ggactgattg gcagtggaca
     3241 gatgggaaat agagtcgaag aagttgcaca agaaagagga gatacggtcc ttcttttacg
     3301 gacggcacct aaagaagcaa ctacgcttgc ttttacggaa ctgccagaac tgcttattga
     3361 tttttctcat ccagataatt tagagaaaat cctttcgtat agcttatcat atcaacttcc
     3421 gttagtcatt ggcacgacag gttatacaca atcacagttc caagaaataa aagaacatgc
     3481 caaacatgta cctgtgatgt atagtgctaa tttttcgttc ggcatcatgg tgatgaatca
     3541 actaatcaag caagcagctg caatgttgca aggctggcag atcgaactga ttgaaaaaca
     3601 tcatagtcgt aaaaaagatg caccatctgg tactgcaaat atgctgcttc aaacaatcca
     3661 agaagtgcaa aagctgcagc ccgtatacaa ctggcaaggg aaaaaaagag aagaaaacca
     3721 aattggtgta cacagtattc gtgcaggttc tttacctggt gaacatgatg tattgtttgc
     3781 tgctactgat gaaatattta cgatcaaaca tgaatctttt tctaatcgaa tctttgctgc
     3841 aggcgcagta gaagcagcgg actggttgaa agatcagtca ccaggttatt acaaattaga
     3901 agatttatta atggacaaag gggtgtaaaa aacgtggaag ctcaagaaat catccgctac
     3961 attcaaacgg catctaaaaa gacgcctgtg aaagtctatc tgaatgtaac cgagccaatc
     4021 gaatttaaag aaagtaaagt ttttggtgaa ggaagcagct tcgttgtttt tggtgactat
     4081 gagacgattg ctccggtttt agaagctgaa tcagaaaaaa tcattgaaat cgaaatcgaa
     4141 actgctgcaa gatacagcgc agttcctttg ctagatatta aaaaaattaa tgctcgtatc
     4201 gaaccaggtg ctattatcag agatcaagtg tcaatcggca acaatgccgt gatcatgatg
     4261 ggagcgatta tcaatattgg tgcagtaatc ggtgaaaaca cgatgattga tatgggtgct
     4321 gtcttaggtg gacgagcgac agtgggcaaa aactgccata ttggtgcagg agctgtctta
     4381 gcaggtgtga tcgaaccagc atcggccaaa ccggttatcg tagaagacgg cgtactggta
     4441 ggtgcgaatg cagtgattgt tgaaggtgtc catatcggaa aagatgcagt cgtggcagca
     4501 ggagcagtgg ttttagaaga tgtggcagca gaaactgttg tcggtggaat acctgcacgt
     4561 gtattaaaaa taagtgatga tcagacaaaa gaaaatacag cattgattgc tgcgttacgc
     4621 gaattataga ggagatggag aaaaatgcgc gaaatagata cctgtattgt gggggcaacc
     4681 ggattagtcg gccaaacgat gctcaaagtg ttagaagaat atgattttcc agtaggacga
     4741 ttgaaattat tagcttcaaa acgttcagcc ggtaaagttt gtgtattcaa gaaaaagaaa
     4801 cacactgtac aggaattgac agaaacttca tttgaaggat atgatttagc gctattttct
     4861 gcaggtgctt ctgtatccaa agagtttgcg ccaatcgcac gaagtcaggg actatttgtc
     4921 atagataatt cctcagcatg gcgggaagat tcggatattc cattaatcgt tccagaggtc
     4981 aatctggaag actatacatt gaatcggctg attgcaaatc caaattgttc aaccattcaa
     5041 gcggtacttc cgcttaaagc tttacaagaa gcctttggta tccagcgagt caattattcg
     5101 acctatcaag cagtttctgg ttccgggcaa aaaggcattg aagatcttca tcgtacgcaa
     5161 aatgacgagg aaccagtttt gtatccttat aatattagcc aaacggtgat tccggaaatc
     5221 gatcagcctt tggaaaatgg gtatacaaaa gaagaacaga aaatgataaa tgagacccgt
     5281 aagattctgc atcagcctgc tttgccagta tcagcaacct gcgtacgtgt gccaatcgaa
     5341 aatggacatg gagtctctgt ggcagttcaa ctgcaaaaag agtttacgac ggaagaaatt
     5401 agagagtgtc tcagccagtt tccagggatt caattagaag atgatctagc agcacataaa
     5461 tatccgacga gcattcttgc tagaggaaca gatatggttt atgttgggag gatcagaaaa
     5521 gacacttctt tagagaacgg attgttgttc tatacgactg ccgataacat acgcaaagga
     5581 gccgctgcga atgccgtaca gattgctgca gctttaatgc agaaaatagc ggaggtggca
     5641 agatgagtaa tacgctgaaa gtagcgaaat tcggcggaac atcgatgtct catgaagagc
     5701 aattcaaaaa agtccgtaca attgttcttt cagatcccaa tcgccaagtg atcgttgttt
     5761 ctgctttagg caaacgtacg tcagatgatg acaaagtaac agacttactt tatttgattt
     5821 atgcttacct tcagcatcat gttgattgga cacccctttg gaaaaggatt tgtgatcgtt
     5881 tcattcaagt aagagatgtt cttgccctgc agttacctat tgaagatgaa ctagcacgca
     5941 tacagaaaca actggaagac gaaaagataa ctgaagatta tttagtaagt agaggtgaat
     6001 atctgactgc tcaactgatg gctgagtatc ttggttacca gtttattgac gcaaaagacc
     6061 tgattttctt tgactatgat gggaaaatcg atcaggaaaa aacagcacag gaaatcacaa
     6121 aagcatttaa aagatatccg aaaatggttg ttccagggtt ttacggggcg aaccctgcag
     6181 gaaaagaaaa attattgggc cgtgggggaa gcgacattac cggcgcgata ctggcgaaga
     6241 tgttgcaagc ggaaagttat gagaattgga cggatgtttc aggaatcatg atggctgatc
     6301 cgcgaatcat cgatcatcct aaaatcatcg aggaaatctc ttttagagaa ctgagagaga
     6361 tggcttatat gggggctaac gttcttcatg aagcagctgt tttcccagtt caagaggctg
     6421 atatccccat ccaaataaaa aataccaatg atcctttcgc tcaagggaca aaaatatcga
     6481 atcacgaaat agaaaaggaa aatggactga caggaatagc tggacgcaag gattttcttt
     6541 ccatcacttt attcaaacgt catatgtccg atgaaatcgg gtttatctgg aaagcaatga
     6601 gcatttttgc taaacatgag atatcaatcg aacatattcc ttcagggatc gacaatatcg
     6661 gagtgatcgt ctcagcagaa gcgattgccg ataagttatt tctcatcacg aaagaattaa
     6721 aagaaacact tggcgtggaa gaaatcgtcg tgatcgaaga cttagctttg atctctgtcg
     6781 ttgggggtcc gcataaagaa ttgattggat tatctgggaa agtgttatcg atattgaaca
     6841 aattagaaat tcgcacatcg atcttatcac aaggagcaca ggagttgaat ctgatcatcg
     6901 gtgtaccgaa taatcaatat gaaacagtag tgaagggtat ctatgaaggg atggtgaata
     6961 cggatgctga aagaatatcg aatggcactc catcaaattc ctgaaatcgg gtttcatgaa
     7021 ttcaagacaa aaaaatattt atatgaacaa gtaaaaaaca gcggggccgt tatccatgaa
     7081 atcggcgaaa caggactact cctttatttt gataatcagc aagaagaaac gattgctttt
     7141 cgaacagata tcgatgcact gccagtcaaa gaagaaacga atctctcttt tgcttctaag
     7201 catgaaggtt atatgcatgc atgtggtcat gacggacata tggcgatgct tttagggttg
     7261 actgacttta taactaaaca cattgaggat cttatttata atgtagtact gatctatcag
     7321 ccatcagaag aaatttccgg tggcgcacaa agcgttattc agtcaggatt gttggataaa
     7381 tatgaagtac aagcaatatt tggctttcat ctatggcctg gactagctaa gggagaagtt
     7441 ttctctcgac caggaccttt gatggcacaa agcagtgaaa cagacattat cattaaaggc
     7501 cagtctgcac atattgcttc tagtgaaaaa ggaatcgata gtctggaggc agctgtacga
     7561 tttttgaaga atgtttatga ttttgaccgg caattaccgg cagagtggaa tcatctaatg
     7621 aaatttggcc aaatccaagg tggaacgatc cgcaatgttt tagctggaga agttattgta
     7681 tccggaagta tccgttctta cagttcagat atgcaaaatt atttgaagag gggattagac
     7741 catttagcag aacagtttca ctctacagct actatttctt ttcgctataa tgacggctat
     7801 ccagctgtaa tcaaccatag aggcttatat gcagcactgt tagattcaaa caaactacat
     7861 gaattagatg ctccagttct tcaagccgaa gattttggca tatatacgca acaatatcca
     7921 tgtgtatttt tctttttagg tgtcggtgat acccctgctt tacatgatcc gacgttcgat
     7981 tttgatatgg atgttttaga aaatggtgta gaatggtaca aaacgattct ttttacagaa
     8041 aatcttcttg taaaaatcga tagcagttca aaagaggagc gagtaaataa tgatgaattt
     8101 atccaatcga tttagtccta ggctttctag aatagaagta tcaaaaatac gacaattcga
     8161 tcagcaaata tcgtcgatac cagatgtgat caaactcacc ctgggagaac cggattttcc
     8221 aactcctgaa catgtcaagc aagcaggaat agcagcgatt gaagaagact tttctcacta
     8281 taccggaatg agaggattag aggaactgcg ggaagcggct tgtatatttc agcaacagcg
     8341 ttatggatta acttatgatc cgcaaacgga agttttgacg acagttggtg caacggaagc
     8401 gattgcctct gcgttgttat ctgttttgga agaaggagac aaggttctta ttccagcgcc
     8461 tgcttattca ggatatcagc cattagtgga actagctggt gctgaattga tcccgataga
     8521 tacttctgac acagggtttg tctgtcagcc ggaacaattt gaacgagcat ttgaacaata
     8581 tggatcagct gtcaaagcag tcattttaaa ctatcctaac aatcctactg ggacgacatt
     8641 gtctgcaaaa caattgaaag caatcgctga ggttttaaaa aaatatccgg tatttgttat
     8701 aagtgatgaa gtatatgctg aattgactta tagtggaacg catatgtcca tagctactta
     8761 tcttccagaa caaacaatcg tgataagcgg gttatccaag tcacatgcga tgactggttg
     8821 gcgagtgggc tttatttttg ctcaaaaagc ccttattgac gaactaatta aagtccatca
     8881 atatctggtg actgccgcta caacgatgtc gcaaaaagct gcggctgaag cgctaataaa
     8941 tggggtgaaa gatagcgaga atatgaaaga gcaatatcaa acacgaagag actatctggt
     9001 ccagcaattg gcgcctttag gatttgaagt gacccagcca aacggtgcat tttacttgtt
     9061 ttgcaaactc ccagctgcca ttcaaactga ctcttggcag ttttgtgtag aattagcgga
     9121 acaggcaaaa gttgcgtgta tcccaggaat tgcatttggc ccagaaggag aaggttatat
     9181 ccggatttct tatgctagca gcatggaaaa attacatgag gcctgcaaac ggatcaaaga
     9241 ctttttaaca gaacagtgat tcatagattt tatcaaaaaa acaatcgtta attttgcggt
     9301 atattctgca aggagacgat tgttttttaa atttagttca aatgaacgcg tttcttttga
     9361 ttcataacta atgtaatgtt aagtttaaat tgtttagaat gattttaaaa gaatattcaa
     9421 ggaggtttca tcaatggaaa aaatcaaagt tgtcattgta ggtgcatctc atggagggca
     9481 tcaatcgatc ttggaactat tgacacgata cggtgaaaat gtagatatca cgctatttga
     9541 agcaggagac tatatttcct tcatgtcatg tggtatggag ctgtatttag aagacaaggt
     9601 aacagatgta aatgatgtac gtaatttcag accagaaaac tttccgcaat ccaatgttca
     9661 tattttaaat aatcacgaag taaaaacgat cgatgccgat aaaaaaacag taacggttac
     9721 tagcgcatca gataacgaaa caaaagaata tccttatgac aaattgattt tgagttctgg
     9781 tgtcaaacca aacaatctgc cagtaccagg agcagatcta gaaaatgtct acttgatgcg
     9841 tggatacaat tgggcaacca aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat
     9901 cgctgtcgta ggtgcgggtt acattgggat cgaagcagca gaagcaagcc gaaaagctgg
     9961 gaaagaagtc gtattgcttg atgtgatcga tagaccttta ggcacttatt tagatgctga
    10021 gatgacagat attttagaac aacacctaaa tgaaaacggt attgaagttt tgacaaatgc
    10081 aaaaatcgaa gcttttactg gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga
    10141 aatcccagca gatctagtca ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa
    10201 agggatcgtc gacttagatg aacgtggctg gatccaaaca aatgaatact tgcaaacgaa
    10261 tcttccagat gtgtatgcag taggagatgc tacacttgct tattctatcc cagcacgcaa
    10321 aaaaatgccg atcgctttag cgactgttgc acgtagagaa gcacgttatg tagtaaaaca
    10381 tctttttgaa gaagtaccaa acaaaccgtt cggcggagtc gtaggttcct ctgcattaag
    10441 tgtctttgac tatcattttg cagcaagcgg attgaatagc tttacagcag caaaagcagg
    10501 agtagatgta cgagtgtctt attatgaaga tacgttacga ccaaaatatg taccagaaga
    10561 acaaggaaat ccaaaagtgt ccatccaatt attctcggat ccaatgaccc atcaattgct
    10621 aggaggagca gtattatcta cctatgatgt tacaggtcaa ggaaatgtat tggcactagc
    10681 gatccaaaac aaactgactt tagaagaact agcagaagct gacttcttct tccagccagg
    10741 ctttgaccga caatggagca tgttgaatct agccgctcaa caagcgttag gagaagaacc
    10801 atttgtagaa taagaaaacg aaatatttga aaagaggttg tgaaaaagct gccttgcatc
    10861 aagtaataag aacagccaag taaaaacagc ttcttatgtt tttaacttgg ctggacttat
    10921 taccgcagat gcaagctttt gaacaccgtt tattttcaaa aaagggactg tgacacgatt
    10981 tgtgtaccga cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt
    11041 atggttaaat atacttttga acttaaatta aagatcgttc atgattattt ggatggcaaa
    11101 ggcgattgga ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaaattcggg
    11161 agaagcacaa ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct
    11221 ctatcaataa aaagaaagtt cagcgcttga tcaagaagct aggaataaaa gtaacggcat
    11281 acacgcgtaa atcacgtcgc tacaattctt atcgaggaaa agt
//
LOCUS       DANJLF010000076        10817 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000076 DANJLF010000000
VERSION     DANJLF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(25..162)
                     /locus_tag="PWT25_002288"
     CDS             complement(25..162)
                     /locus_tag="PWT25_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL1022335.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="PWT25_002289"
     CDS             complement(159..1343)
                     /locus_tag="PWT25_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL1022336.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="PWT25_002290"
     CDS             complement(1599..1853)
                     /locus_tag="PWT25_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022337.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="PWT25_002291"
     CDS             complement(1878..3020)
                     /locus_tag="PWT25_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL1022338.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="PWT25_002292"
     CDS             complement(3080..4432)
                     /locus_tag="PWT25_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL1022339.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="PWT25_002293"
     CDS             complement(4503..4847)
                     /locus_tag="PWT25_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL1022340.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="PWT25_002294"
     CDS             complement(4857..5231)
                     /locus_tag="PWT25_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL1022341.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="PWT25_002295"
     CDS             complement(5244..5558)
                     /locus_tag="PWT25_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL1022342.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="PWT25_002296"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="PWT25_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="PWT25_002297"
     CDS             complement(8976..9638)
                     /locus_tag="PWT25_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022343.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="PWT25_002298"
     CDS             10255..10485
                     /locus_tag="PWT25_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022344.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="PWT25_002299"
     CDS             10491..10790
                     /locus_tag="PWT25_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022345.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DANJLF010000077        10721 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000077 DANJLF010000000
VERSION     DANJLF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(238..1086)
                     /locus_tag="PWT25_002300"
     CDS             complement(238..1086)
                     /locus_tag="PWT25_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1022346.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1085..2152
                     /gene="gnd"
                     /locus_tag="PWT25_002301"
     CDS             1085..2152
                     /gene="gnd"
                     /locus_tag="PWT25_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL1022347.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2154..3155
                     /locus_tag="PWT25_002302"
     CDS             2154..3155
                     /locus_tag="PWT25_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL1022348.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3152..3436
                     /locus_tag="PWT25_002303"
     CDS             3152..3436
                     /locus_tag="PWT25_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL1022349.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3469..4266
                     /locus_tag="PWT25_002304"
     CDS             3469..4266
                     /locus_tag="PWT25_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1022350.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4280..5113
                     /locus_tag="PWT25_002305"
     CDS             4280..5113
                     /locus_tag="PWT25_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1022351.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5131..5988
                     /locus_tag="PWT25_002306"
     CDS             5131..5988
                     /locus_tag="PWT25_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1022352.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6010..6519
                     /locus_tag="PWT25_002307"
     CDS             6010..6519
                     /locus_tag="PWT25_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1022353.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6556..6957
                     /locus_tag="PWT25_002308"
     CDS             6556..6957
                     /locus_tag="PWT25_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022354.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7069..7422
                     /locus_tag="PWT25_002309"
     CDS             7069..7422
                     /locus_tag="PWT25_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022355.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7456..7899
                     /locus_tag="PWT25_002310"
     CDS             7456..7899
                     /locus_tag="PWT25_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL1022356.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7916..9001
                     /gene="uxuA"
                     /locus_tag="PWT25_002311"
     CDS             7916..9001
                     /gene="uxuA"
                     /locus_tag="PWT25_002311"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL1022357.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8994..9707
                     /locus_tag="PWT25_002312"
     CDS             8994..9707
                     /locus_tag="PWT25_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1022358.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9914..10063)
                     /locus_tag="PWT25_002313"
     CDS             complement(9914..10063)
                     /locus_tag="PWT25_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022359.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10459..10659
                     /locus_tag="PWT25_002314"
     CDS             10459..10659
                     /locus_tag="PWT25_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1022360.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3585 a         1695 c         2320 g         3121 t
ORIGIN      
        1 aataataaaa aaaagaaaag ataaattttt tactactgta tactaacttt gttatcgcat
       61 ctaacaagta actcgaataa taccaataca accttctcca aagatgcgat gacatacgtt
      121 ctaacccacc gactattgat gttctgtacc acccttgcag aaccaaaaaa gccactacca
      181 aatatttggt agtggctttt tttgttttat ggatttttcg ctttttctgt tatggtgcta
      241 ttctttgatt tcagaaatag tatgtcgaac tttagcgata gaacgacgtg attctttttc
      301 gttgtaaaac atcaaaatca catatagaga gtccaaaatg cttagttgag aaatacggga
      361 agctaaagct tcagatcgga attctgtttc ttcagaaata gaaataaaaa cgacatcgcc
      421 aagtttagca agggaagaat tagcttggct agtaataacg attgtttttg ctccagtttt
      481 tcttacggtt tcagcaatat ggattgtttc ttttgattgc cctgtgtgag aaatcaaaac
      541 agcacagtct ttttccgtca ataaagaagc ttccatcaat tgaatatggt aatccgtgct
      601 gtgagaagtt gcgatagggg aacgcaaaaa tttatgataa gcgtcagttg ctacaatctc
      661 tgaaccgcca acaccgaaaa aatagacacg tttagattca ttgataatag tagccgcttt
      721 ttttagatcc ttttgttgta acaacttttt tgtgtcattt aaagttgaaa tactagactc
      781 gaacaccttt tgcgccatag tgagctcagt atctgttttc tgaatatttt cgtggataga
      841 aatagcagat agatcatttt cttggatcaa catggccatt ttaaaatctt taaatccatt
      901 ataaccaagt ttttttgtaa attgaaaaaa agtagagtca gcaatgccca gttcacttgc
      961 taaattgcta ataggactat gacttacttt gcttggattt tctaaaatat aatcggcgat
     1021 agcctgttct tttgggctga agtcgttgta cattgttttg atttgtagtc ttatggagac
     1081 gctcatgaga attcctcatt ttcataataa gattatcatt ttttccgttc tcattaatgt
     1141 taaagataca aggagagtag gaaaaaatca aacaaaaaat atttttgtaa aatctattta
     1201 caaaaaaatt tttttgtata atgagggtat aagaaatgga ggcgctatca tggaaattgg
     1261 tgtaattggt ttgggaaaaa tgggattgaa tttagcttta aacttaaaag agcaccatca
     1321 caaagtacaa ggactcgaca tttcaagtac agctgttagt caggcaagag aaaatgacat
     1381 tcctgcttat caagagatct ctgaatttct agctactttt gagcagaaaa aaatcatctg
     1441 gttaatgctt cctgctggag agataaccga aaacatgtta agaaaacttt ttccaaaact
     1501 cggaccagga gacattatta ttgaaggcgg aaactcttat tataaagatt caatccgtcg
     1561 agcagaagag tttcagaaaa atgaaatcgg ttacttggat tgtgggactt ctggaggaat
     1621 cgaaggagca cgacatcaag cctgcttaat gattggagga gataaagaaa cttttctatc
     1681 cgtggaacaa ttattcaaag atgtagcaat tgaaagagga tatctgtatg ctggtgcatc
     1741 cggaagcgga cacttcttga aaatgatcca taatggaatc gaatatggaa tgatgcaagc
     1801 aatcggtgaa ggattccaac ttgtacaaaa aagtgagtat gcttttgact tggaacaagt
     1861 cgcacgtgtt tggaatcatg gttcggtgat tcgcggctgg ctgatggaaa tcatggaaga
     1921 tcagttaaac gaacatccca atttaagtaa ttatcgggga atcgtttctg catcaggtga
     1981 agcaaaatgg acggttgaaa cagcgctaga aatggctgtt ccagttccag taattgcaac
     2041 tagtttgttc atgaggaatc tttcacagga agaagatagt ttttcagcaa aagtagtttc
     2101 agcactaaga aatgggtttg gcggacatga aatagttgaa aagggtgagt aaaatgccag
     2161 attatcatat agcaatcgtt aattccagca gtttcggaag agtctttcca caccacttgg
     2221 aaagattaaa gtcagttgga gacgttcatt tctttactgt tgatcaagag attccaggaa
     2281 aagaactagc ggagttactt aaaggattca atatcattat tgcaagtgtt acgccatttt
     2341 ttcgacgaga tttttttgaa aacaaagatg aactgctatt gatttcccga catggtattg
     2401 gttacaacaa tattgattta aaagcagcaa aagaacacca gacaatcgta tctatcatac
     2461 ctgctttagt tgaacgaaat gctgtagcag aaaataatat aactaatttg ttgtccatgc
     2521 ttcgacgcac gacacaagca caagcaagag tcaaggacga tcgatgggaa gatcgggcgg
     2581 agttcgtcgg taggacgctg tttaacaaaa cagttggtgt gattggtgca ggaaataccg
     2641 gaagcggagt aatcgaaatt ttgcgtaacg gttttcgctg tgatgttcta gcttatgatc
     2701 cttacaaatc agatttatac atcgaaagct ttggtgcaaa aaaagtagaa cttcaagaat
     2761 tactggaaca atcagatatc atttgtttat gtgcgaattt gacagaagag aactatcaca
     2821 tgatttctga taaagaagtc gatcagatga aagaaaatgt ttatctatct aatagtgcaa
     2881 gaggcgctct agttgatgaa gaagcgatga ttaatggtct gaaaagcgga aagatcgcag
     2941 gatatgccac agatgtttta gaagaagaac cgggacgtca agatcatccg tatttagcgt
     3001 ttgaacaagt agtgattacg ccacatatat cggcctacac aagggaatgt ttagaagcaa
     3061 tgggtgaaaa atgtgtcaga gatgtcgaac agatcagtca gcaccttctt cctgaacgtt
     3121 ctgtccagac aaagagcaac tatataaaag gatgatgaag aaaatggtga acggtttaaa
     3181 agtgaaaaca ggccctcaat tttatttata tgaagaaggt ggtatttcaa aagtttctga
     3241 tctcttgaaa tcatacggcg cgaaaagagt actggtcacc cacggaacgg tatcatggga
     3301 aaaggcgcta cctaaattag tatttttgaa tgacgaaacg attcaatttt tttatcatcg
     3361 ttattctgga gaatgtagtt atgctgaagc cagacgaata gctacaatca tcaaaaaaat
     3421 gaaatcgatt tcgtagttgg cgttggcgga ggaaaactga ccgatctagt gggttatagt
     3481 gccaatcttg caagaatcga tttcggagtc atccctactt tagctagtaa ttgtgctcca
     3541 tggacacctc ttgctgtcat gtataaggag aatggacaag cagaagggaa aacagaacac
     3601 ttttacagac aagctgcttt ttttataaca gatccccaat tagtgattga ttcccctgtt
     3661 ccatatttca ttgccgggtt agcagataca ctagcaaaat ggtatgaatc tgagctcatc
     3721 ttagaacaag attttttgca agatgaatca tttttgaagc tagcacaaga tactgcgaag
     3781 atctgcaaag aagagatttt gaatcattct gctaaagcaa tccaagatat ggcacaaaga
     3841 aaactgacat cggagttcaa acagctttca gagattatct ttgctgttgc tggtttagtt
     3901 ggaggattag gagataaata cgcaagaaat gcggcagcac atgctatgca tgacgctatc
     3961 agtaaatata ttccggaaag ccatcgtttt ctacatggag aaaaagtagc gtatgggatt
     4021 ttctatcaac tggcactaga aggtcgctgg gaaaaaatag acgagttata tcctttgtat
     4081 cgggaattag aactgccggt ttctctagaa caaatgggat tatttccgaa agataaacag
     4141 ataatagata aaatggctga atttattgat tcgaaagaga aagtgcattt gattcctata
     4201 gaggtaacac agaaatcact gatacagtca atgatggaac tggaaggtta tatcagaaag
     4261 atctgaagga ggaggacaca tggaaacatt aagtgtagta caaagtttgt tgattgcttt
     4321 atgggtagcc gcggttatgt cccgctggtt aggtggagga gccacgttga ccttacgttt
     4381 ttctcctttg atgactggat tagtcgtcgg gttgatcatg ggggaagttg cacaagcgat
     4441 gattgttaca gccgctttgc aaatgattta tatggggggt ttttctccag gaggatcaat
     4501 gccagctgaa ccatctattg cagcagctat cgcagtacca gtcgcattat taggggatct
     4561 gtcgccagaa gcagcgattg ccgttgcggt gccagtcgga ttgctcggaa gttacttgta
     4621 tcagtttcgt tttttcatca atactttttt aggtaaatat acagacaaag ctgtagaaga
     4681 tttgaataca aaagctatgg cccgatcaat tattctttat ccaacggttg cctcattttt
     4741 attattcgtt ccgcttgttt tcattgcttt atatttaggg gcgccattga tcgctgatgt
     4801 aatcaaagct ttagagggaa cagtcgttat ccacattctt gaagtagtag gtggagggtt
     4861 agcggcaatc ggtatcgcga caactgtata cgttatcggt cgaaaagatt atctggtctt
     4921 tttcttcctt gcttatttta tgagtgttgt tttgaaatct ttagatatta cgatggttac
     4981 ttatgccatt tttgggatta ttatcgcttt gatctttgtc cagtcgcaaa aagggaaaca
     5041 agtacagcct gccacaagca ataacaattc agcatctttt gatgacgaag atgactatga
     5101 tgacggattt taatttagga ggaatgggaa atggctgaaa taatggataa tcaaacaggt
     5161 gctcctgaag aaatcacgaa aaaagatgta acaaaagcct atctgagatg gcattttgca
     5221 aacgaaattc ctcactcttt tgaacgttac ttggcgccat cactactata cggaatgatg
     5281 ccaatcttga aaaaacttta taaagatgaa gactctttaa aagcagcata taaacggcag
     5341 ttactgttct tcaacactca attaagctgg ggtggcggtg tgattactgg cctaatgtct
     5401 tctatggaac agcaaagagc agaagaagag tatgaagaaa aagagatctt gatgcaggat
     5461 gacttgatgt ataacacgaa agcgggattg atgggggcac tagcaggaat cggagatgca
     5521 atcgattcag gaaccgtcca atatattttt atcgcaatcg cggtaccttg ggcacaacag
     5581 ggaagtcctt taggcgcact ttttcctttt atctgctttg ggttgtatca ggttttacta
     5641 ggggtatttt tcgcaagaca agctttcact atgggccgta atgctacagg cctaatgcaa
     5701 aacactggtg ttcagacagc gattgaaatg ctatcagttt tagggttgtt tatgatgggg
     5761 gtgcttgcgg gaaactatgt aaaagtagaa tcttcattga aatttgcgat atccggacgt
     5821 gagtttgtta tccaggaaat tcttgatcaa atcgtgccag ggatattacc tttagctgtc
     5881 gtaatgggtg tgtacttgtt ctatacgaaa aaaggattga aagtcacaca agcattatta
     5941 tggctgactg gaatattaat tgtgctagca ggaatcggta ttttataaaa agaagggaac
     6001 gtgaataaga tgggtgtagt caatttagcg cgtgtggatg agcgtttgat ccatgggcaa
     6061 gtaatggtaa cgttatctca aaaaagtgga gtgaattcca tttttgttgt agacgaagtt
     6121 gtagcaaaag ataaatttat gagagatctt tacaaaaatg caggaagccg tacaggacaa
     6181 aaaacaattg tcattacacc agagaaagct aaattctatt gggatgaata tcaatttaaa
     6241 gaatacaatt gtattttgat cgcaaaaaca gtccaaacga tttacgactt agtaaaacac
     6301 ggaatcccca tggaagaatt gaatattgga gggattgctc aaaaaaatcc tgatacagat
     6361 cttttagtga cgaaatctgt ttatttaaat aaatcagatg ctgaaaaatt gaaagaaatg
     6421 aatgaacaat atggcgtaaa gaatatttat tttcaagcca ctccatctgc acctaaaaca
     6481 ggattaaaag atgtattgaa caagtttgat ttgtcataaa caaatgctta gaaaaaagat
     6541 aaagagggcg aaaaaatgaa gaagaacgag ctgtttcgtg attgggaatt tcgttatcgc
     6601 tatatttacc gtaagcgccg tacaaaaaag agtaaacagc gttttctttc agcattagta
     6661 tcagatattt acagtatgag aacagatgtg acagtcattg cttacgatac gctagcctac
     6721 cgatcaaaaa atatttatgt tggagatatc gaaaaagcag agaaagttat ctgtacttat
     6781 tacgatacac ccgttcatgc attaggaagt tatttcatgt ttgattggaa agaccaaagg
     6841 aaaaaaacaa tctactctat attattgtcc ttcattttgt tgttcagttt aggttggtgg
     6901 ggaatgatga tatataacaa aaatcctcat cacgtattcg atctgctatc cgtataaaca
     6961 ggtatcactg tactagcatt tgggagctac tttttcttat tgggaaaagc agctcgcgga
     7021 tggcctagca gacaaacttt tattcgaaat acttcatcca tattaacgat gctagaaatg
     7081 atccgtacga ttgatgatcc gagtgtagcg tatgcgtttg ttgatgaagg ctgttatgga
     7141 gaaaagggct tagacagcgt gagatcgggt atgaaaaaag aggctattct tttttatcta
     7201 gactctgttg gggcagatac accgctacag ttttcgggaa attatttttc aaataaagaa
     7261 cagtggctga aacaagttga taagttgaaa gaaaaaaacg tcaattatat ttttagtgca
     7321 agaaaaaagc aagcgcagtt cttctatttg accaaaacag atttacgagg gaaaacattt
     7381 aattggcaaa atgccaatca gattattgct ctttttcgat aagtaaaaag tgcaaaacaa
     7441 ggagtgagaa gactaatgtt ggaaatcatg atcgttacac atggtaagtt gagcgaagga
     7501 ctgaaagatg cggcggaagt aattgtcggg acatcgagcc atattgaaac aatgtgctta
     7561 gagtctgggg aggacatcca aacattcgga gagaaggtca aagaaaaatt aactatgatc
     7621 aacacagcag aacccatgct agtatttgtt gatctaatta gcgcaagtcc gtacaatcaa
     7681 gcgctcttgg cagtcaatga actgactgaa gaaaagaaag cagccactta tgtcataggg
     7741 ggcgtgaatc tgccaatgtt gttagaagca atcaatcatc agatgcttca cacaccgata
     7801 gaagaagcgg tggaacaaat tgtcaaacaa ggaaaagata atttaactgt ttggcatgct
     7861 tcagcggaag aaaaaaaatt agatgaggac gatttttaga aggaaggctg aaaagatgaa
     7921 gtggggattt agatggtatg gtgaaaaagg ggattcgatc ccattaaaat atgttcgcca
     7981 aataccggga ataaatggga taattggaac tttgctgact aaaatgcctg gagatgtttg
     8041 ggagatttca gaaattgagg agttgaaagc atctgttgag agccaaggac tggaactgct
     8101 tggaattgaa agtgtagcga tccacgatgc aatcaaggca gggacagatg aacgagatta
     8161 ctatattgat aactatatac gaacaatcaa aaacttgtct cgatgtgggg ttcgattgat
     8221 ttgttatagc ttcaaaccaa tttttggatg ggcgaaaact gatttgttta atgaaaacaa
     8281 cgatggaagc tattctcttt tatacgatca gaaaatcgtc gatcagatgg agccaggaga
     8341 aatgtatcaa ttgatacaca gccagtcaaa gggattcaaa ttgccgggtt gggaagaaga
     8401 acggttgaaa aaattcgctc agctggaaaa actgtatgaa ggaatgaccg aagaagaatt
     8461 atttgaaaat ctgacttatt ttttagaaaa agtgattcca gtgtgcgaaa aagcagatgt
     8521 aaaaatggct atccatccag atgatccgcc atgggaactt tttggttttc ctagagtgac
     8581 gaaaaatctt ggcgacttga agaaaattct aaatgcagta gattctccat ataatggact
     8641 aacgctgtgc acaggatctt taggggcaaa tccggaaaac gatttagttg aaatcatcca
     8701 tgaggtaggt tcgcgcatcc attttgtcca cttcagaaac gtggagtttt taggagaaag
     8761 aaagttcaaa gaaacggcac atccctctac agaaggctcc cttgatatgt atcatattat
     8821 gaaagcgttg gtgcaagaag ggttcgacgg tcctatacgc cctgatcatg gtagaaccgt
     8881 gtggggtgaa gtggctatgc ctggatatgg cctgtatgat cgtgcaatgg gactcactta
     8941 catgcaaggt ctatatgagg cgataagtaa ggaaaaacca ctgggagagc attatgaata
     9001 aaatcaagct cttcatcttt gatatggatg gccttttatt agaaactgga cgtttagctt
     9061 atcgtgctta tgtaaaatca gcacaaaagt atgattatga aatgcgtaag gaagtctatt
     9121 atttactaac tggccaaaca gaaatggcga taagagaaca aatgggcatt ttgtacggag
     9181 aagatgttcc atatatcaaa tggcgagaag caatcaatca atataaagaa aaaatcgtga
     9241 aagaagataa gagagtatat acgaaaaaag gagcagaaga aattttatct tttgcaaaag
     9301 aaagaggaat ccatacaatc gtggcttcgt ctaatacacg ggtaaaagta gaaatgtatt
     9361 taaaaatgga aaatctatat gccttgtttg atcaaatcat ttcaggggat gatgtaaaaa
     9421 aaggaaagcc ggaaccagag atctttttga aagcttgttc aaaaatgaat attcctccaa
     9481 gtgaagcact tgtatttgaa gactcgattg cagggatcga agcagcaagg cgagcgggca
     9541 ttttgtcttt tctcatcaaa gatcatttga tagggttgcc cgatcataat gggaagcata
     9601 agttgaaagt agatgtaagt ttatatgaat ccaataaagc agactatgaa ttttatgacc
     9661 tgcatcaagc aaaacgattt ttagaaagta aagggttgta tctctaataa agaggactat
     9721 tttctagtcc tctttattta ttgtcctgca cgactgaggg aacagatgaa aaagaagtaa
     9781 ttttttattt tgaagtatct accttatttg tagtgaacaa tttagaacaa acacaaaacg
     9841 catctactgt tatctaccag tagatgcgtt taaagagaag aacgagaatc gtccatatga
     9901 gggtttccac tccttatgca gattttttta attcttgcac tgcttgttcg atcgtaacac
     9961 ctgtcgctac atgattggta ttttctttgt caaaaacgac aaatagattc aaatctgtat
    10021 ccaaggttac tcgatattgt atatctttgt tatagaatgt catagccatt ctcctttctt
    10081 taaatacttg ctgaaaggca acgaacgcgt tgataacgaa attccattta taagtatacc
    10141 accaattgaa aggttttcat agttaagaaa ttttaaagtc aaaagttgca gataaaagca
    10201 aataaacaag aaaacaaaaa gtgaatcaat cgcggttatt caactttttc ttcgattcga
    10261 agacaataga agacaatgaa ttttatattt cattgcatta tccttgtgtt attagaaaaa
    10321 gcatgatata ttaaatgagt aatctatttt gaaacgtaat ttgagcgata tatctacata
    10381 gataaaactc cttttgccta tcaaatattt attgcaacga aaaaacgtac attgatacgt
    10441 atcaggagga atttatttat gaataacggt acagtaaaat ggttcaacgc agacaaaggt
    10501 tttggcttta tcactggtga agatggaaat gatgtatttg cacatttttc agctatccaa
    10561 ggtgacggct tcaaaacttt agaagaaggc caagcagtgt cttacgatgt tgaagatggt
    10621 caacgtggcc ctcaagcaac aaacattgtt aaagcttaat aaaaattgac ttttaaccgt
    10681 cttttcgaaa gaaaagacgg tttttttgta cgaaaaaatt c
//
LOCUS       DANJLF010000078        10509 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000078 DANJLF010000000
VERSION     DANJLF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            30..653
                     /locus_tag="PWT25_002315"
     CDS             30..653
                     /locus_tag="PWT25_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022361.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1103..2152
                     /locus_tag="PWT25_002316"
     CDS             1103..2152
                     /locus_tag="PWT25_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1022362.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2149..3030
                     /locus_tag="PWT25_002317"
     CDS             2149..3030
                     /locus_tag="PWT25_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1022363.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3033..3803
                     /locus_tag="PWT25_002318"
     CDS             3033..3803
                     /locus_tag="PWT25_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1022364.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4082..4936
                     /gene="aroE"
                     /locus_tag="PWT25_002319"
     CDS             4082..4936
                     /gene="aroE"
                     /locus_tag="PWT25_002319"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL1022365.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5114..6277
                     /locus_tag="PWT25_002320"
     CDS             5114..6277
                     /locus_tag="PWT25_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL1022366.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6394..6804
                     /locus_tag="PWT25_002321"
     CDS             6394..6804
                     /locus_tag="PWT25_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1022367.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6978..9206
                     /gene="topB"
                     /locus_tag="PWT25_002322"
     CDS             6978..9206
                     /gene="topB"
                     /locus_tag="PWT25_002322"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL1022368.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9377..9661
                     /locus_tag="PWT25_002323"
     CDS             9377..9661
                     /locus_tag="PWT25_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022369.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9821..10057
                     /locus_tag="PWT25_002324"
     CDS             9821..10057
                     /locus_tag="PWT25_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1022370.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10054..10452
                     /locus_tag="PWT25_002325"
     CDS             10054..10452
                     /locus_tag="PWT25_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL1022371.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3664 a         1767 c         2228 g         2850 t
ORIGIN      
        1 aaaaaatagg agaaaaagta gaaaagaagg tgtttgttat ttgggaagta gttaaatcga
       61 gagtacaaga aatgaatgaa catttctcag tgtcattcgc agaaaatcca agtaagtatt
      121 ttcgacgtct tgtttttctg ctcatcttat cattgctgtt aacagttgca atgtttttat
      181 tgccggaaaa aatccgagaa gagcaagtaa atagtctcgt ttattcgtcc ataccaacca
      241 gcaaattgaa accttttgat tacagcaaag ctgatcagaa aatcaaacaa agccgggcga
      301 tatcggtgat gttcagcaaa ccgaacggga aaacctatca ggacatgctt gatgttttta
      361 atgatcctga gcttatggaa gaattgaatc gaccgatctt ttattatccg atcgtttatg
      421 atgtacatga actggaacaa aaatataaca tacgaacgga tgaagtgaca tttattttct
      481 ttgatggagg aaaagaagca aatcggataa ctgcgggtaa gggaggaata actgattttg
      541 ataaggagtt gatccctgaa ctgaatcgac ttccgttagc caatatcaaa caattagaag
      601 aagaattgga tagaacggga gtaacttccc aaaattccga gattgctaat tgagacattg
      661 gcgcgatgaa acggtgggtc tatatagcgc tatttttaat tatcagaaca agtgatgttt
      721 gatcttcaaa ataacaaata aaaaattaaa aaatatagtt gacgaaattc aaaaattcga
      781 ttagacttaa atcaattctt aaggaaaggg tgacctgtaa tgaacttatc aaaacgtatt
      841 tcagcagaaa acaaaaaaaa ttggcgcagc tggcgtagat aagttgtatt aatggtgaga
      901 ctttcccata gatacaactt tttagagtgc atttctaaag cttgtatcta tgctagacat
      961 gaacattgac tgccgcatag attagaatct atgcggtttt ttgtgtaaag acaatgaccg
     1021 ctttggccat tgtctttttt tgtagactgg atacttgcct gattataata aataaaaaga
     1081 ttgactttgc aggaggaaaa aaatgaaaaa caaacgatta ataacagttg tagtaattat
     1141 tttagtttat ttagcaggtg catttattta tggaaaagcc tcaggcgaaa aagaaagtac
     1201 ttcgacgaac cagacaaaaa aaacaacagt tgttggggta ttgcaatacg tgagccatcc
     1261 ggcattagat gagatttatc gaggaatcaa agatggtctg aaagaagaag gattaaaaga
     1321 aggagaaaat cttgagatca aatttcaaaa tggacaagcc gatcagagca agctagccac
     1381 aatgagtcaa cagctagtac aatctgatcc agatgccttg attggaatcg caacacctgc
     1441 cgctcaatca ttagcaaaca cgacttcatc gatcccagtt gtgttaggtg ctgtaacgga
     1501 tcctgtgggg gctggattag tcgcttcttt agagaaacct ggtggtaatg taacaggtgt
     1561 ttctgatcag ccgcctgtta cttcacaaat caagcttgct tctgatttgt tgccaaatgc
     1621 aaaaaaagtc ggaatcttgt atgcttcttc agaggataat tcaaaatacc aagtagcaca
     1681 agcagaagaa gctgcaaaaa aagaagggtt agaatcagta aaatatgcgg tgccttccac
     1741 taatgagatt gcacaaacgg ttcaagtgat gagccgacaa gtcgatttta tctatgttcc
     1801 attagataat accattgcca atgcgatgca aacagtggta aaagaagcaa acaaagcaaa
     1861 tatccccgtc attccatctg tggatacaat ggtggaacaa ggtggactag caacaatcgg
     1921 gatcaatcaa tatcaattag ggcttcaaag cggtaagatg gcggcagaac ttgcgagtgg
     1981 aaaagaaaaa ccggaaacga cgcctgttta tacatttgat caaggggata cagtgatcaa
     2041 tcaatctcaa gctgatcgct taggaatcat tgttccccaa tcgatgaagg aaaaagccaa
     2101 aatcattaca gatgactcgc agcaagaaac atctaaggag gatgacaaat gatcgtatca
     2161 gctatttcac aaggcatgct ttgggcaata ttaggattag ggattttcat gacttatcgg
     2221 atcttagact ttcctgatat gacgacagaa gggtccttcc cattaggtgc tgctgtctgt
     2281 gtgacagcta ttactcatgg ggtagcgccg attgtagcta cattattagg cgtaggtgct
     2341 ggcatgatag caggactcgt tacggggtta ctttatacaa aaggaaagat accggttatt
     2401 ttagcaggga ttttagtgat gtcaggattg aactcagtta ttttgtttgt gatgcgaaca
     2461 ccaaatcttt ctttattgaa tcatccgatt ctacaagaca gcttccatcg ctttgggtta
     2521 cctgattact ttgacacagt ccttcttggc ttcatctctt tgggcatctt gattgctgta
     2581 ctgctattct tcttcaatac agatttgggt caaggctata ttgcgacagg agacaacgaa
     2641 acgatggctc ggtcgttagg gataaaaacc gatcgtatga aaattttagg tttaactttg
     2701 tcgaacggtg tcattgcatt atccggtgcg ctgattgccc agaacgacgg atatgcagat
     2761 gtaaataaag ggattggcgt aatcgtcatc ggtcttgctt ccatcatcat cggtgaagtg
     2821 atcttccaag aattgacgct agcagaaaga ttgattgcta ttgttgtcgg cagtatcatt
     2881 tatcagctat tgattttagt agtaatcaaa ctagggtttg acacgactta tctgaagttg
     2941 ttctccgcta tcatcttagc tatttgtctg atgatcccgc aattgaagaa agcactgaac
     3001 ttaaaaacgg gttttgagaa ggaggcatag taatgactga tatattgaca atcaaaaatg
     3061 gcagaaaagt cgtaaacaat ggaatgaatg aagagaaagt actattggat gaaattaacc
     3121 ttacaatcag agaaggggac ttcatcactg tccttggcgg gaatggtgct ggaaaaagca
     3181 cactgttcaa tacgatcgct ggtacgcttc aactcagtca aggatcgatc tcttttaaag
     3241 atcagctgat cacaaaagat tcagaagaaa aaagagcacg attcctttct cgagttttcc
     3301 aagaccccaa gatgggaacg gctcctcgga tgacagtagc agaaaatttg ctacttgcac
     3361 aaaaaagagg tcaaaaacgg aaattccgtt tacgtaactt gaaaaatcaa aaagaagatt
     3421 ttttcagact atgtcaagaa gtcggtaatg gcctagagca acacttggat actccagcgg
     3481 gtgaattatc tggtggtcaa cgccaagcat taagcttgct gatggcgacg atcacagctc
     3541 ctgagttact attattagat gaacatactg cagctttaga tcctaaaaca gctaaagcat
     3601 taatgcgatt aacaaatcag cggatcaatg aacaaaaatt gacttgtctc atgatcacgc
     3661 atcgaatgga agacgccttg aaatacggaa atcgtctgat cgtactccaa aaagggaaaa
     3721 tcgttcaaga tatttctgaa aaagaaaagc aacatttgac aatgggcgac cttttgcagt
     3781 tctttgaaga agtagtggat tgatttcgac agaaaaattc ctttcagtag aaggacttga
     3841 aaactcattg acatcttcct ttgattcatc ctatacttaa aacaataaac aattaaacat
     3901 atttgggtgt tttgtaggat cacgtagtca atgattttaa caattaaata gaatattctt
     3961 tgctctgaca agtaaacgat gagaaaagaa tttgctactt tgccacctag acgtttcgta
     4021 ctttttcgta tcaaacgcta agtggctttt tttattgaag taagaacaaa ggaggaataa
     4081 gatgatttct ggacaaacca aattagctgg gctctttgct gatccggctt ctcacagttt
     4141 atccccattg atgcacaata ctgcttttga agcaaacaac atcgatgcgg tgtatttagc
     4201 tttccaagta aatccaatga atttgaagca agcagtcgaa agtattcgaa cttttgatat
     4261 gttaggtgta aatctttcga tgccgaataa aacagcggtt attccttatc tggatgaggt
     4321 aagccaggaa gcgcaattga tcggagcagt gaatacgatc gtgcacgaaa agggtcgttt
     4381 gattggctat aataccgatg gtgcgggctt catgcaatcc gtgcaagaag ctgggatttc
     4441 gatcaaaaag aaaaaaatga ctctactggg agctggaggt gcagcaaagg cagtcactgt
     4501 tcaagctgcg ttagacggcg ctgaggaaat cgtagtttat aaacgaaaca accaaacatt
     4561 cgctcaagta aaagaaacat tcgaacaact cagctatcga acttcttgta agataaaagt
     4621 tcgtgacttt gccgatacgc atcaattaag aaaagacttg aaagaaagct gtcttctagt
     4681 caatgcaaca gatatcggga tgggaaagaa aatcaatcag tcaccgctct tagatataag
     4741 catgatgcat gagaatctag cggtttttga tttgatttat gcacctagag aaacacgttt
     4801 attaaaagaa gcaaaaaaag taggagcaaa aggatacaat ggacttggca tgttgatcta
     4861 ccaaggagca atcgcgtttg aattatggac aaaggaaaag atgccgatcc atgtgattga
     4921 aaagctgttt gattaaaaaa ggataataga taaagatctt ttattctcgc ataaacggtc
     4981 tcaagaaaag taatccttta tgtatcgaag catttatttc gttatttttt attaaattat
     5041 caaaaaatga taggcttttg tcgattggtt atcgtatcct ttatttatca gtaagaaacg
     5101 aggaggaacc acaatgagtc ggatggatcg ctataaggac atccataaga aggcgaaacc
     5161 tctgaagcgg gatacgggga gtatttttgc tagaagagaa cgaaaacgaa cgcgtatgga
     5221 aaatgaggaa gatgctaccc gtatttatga aagagagcag cattcatttg aacatttgaa
     5281 ccaatacgag caacagatat acagagatgc accaagagca gaagaacaac aaaaaaataa
     5341 aaaatttttc aatcggcaaa agaagaacaa accgttaaaa cctcgaagaa agcgatcgtg
     5401 gaaaaaaatc attctatcga ttttgcttgt tttgatcctt tattcaggta tctcattttt
     5461 tattggtaaa tatgtagctg aacatgacac aagcttagag gcagcgactt cagaaacctt
     5521 tcatgggcaa aaagcgacga atggtgcgca taatattttg atccttggaa gtgatacacg
     5581 cggagaagat gccgggcgag ccgatacgat catggtctta cagttggatg gtccttccca
     5641 taagccgaaa ctgatttctt ttatgcgtga cagcttcgtt acgatcccag gggtaggaca
     5701 aaataagatc aattcagctt atgcatacgg aggagccgat ctagtgcgtc aaactttagt
     5761 agaaaatttt ggaatcgatt gtcaatacta tgcgaaagtc gatttcaaat cgtttgaaaa
     5821 agtgatcgat gccttattca tgaacggagt gaagatcgat gcagaaaaag atctgaatct
     5881 ggatggtgta gatattaaaa aaggggtcca gaagatggat ggacatgttc ttttgcagta
     5941 cgcacgtttc cgaatggatg aacaaggaga ctttggccgt gtcagacgcc aacagcaagt
     6001 aatgaacgct attttcagcc aattgaagaa tccattgaat ctgatccgtg caccttatgc
     6061 agctggtaaa gctttaggat atacatccac agatgtcact gcttggttct tgacgaaaaa
     6121 cctcttttcg attgcacgag gagctggtgg cattgatcga ataagtgtgc cagtagacgg
     6181 ttcgtggaat tatgggcaaa gtgattatgc tggcagtatt cttgaaattg ataatcaaat
     6241 gaatcaatct gctattacag attttttgaa taaatgatca taaaaaagat caagaagaag
     6301 ctgcgcaagg cttttgtcat gaatcaatca aacgattcac tcgtaaaaaa cgctttctta
     6361 tggtagattt aaccaagaga ggagctgttt acattggata tctatctgaa cttcaaaaat
     6421 gagtcgcgtg aagccatcgc tttttatgaa aaagtttttc agactgaagc atcaggtatc
     6481 accacatacg gtgaaatgcc tggaatagaa caaatgaact tgccagaaga agcaaaaaat
     6541 ctaatcacga atgcacggct gaacatttat gatacattag ttatgtttgc agatacgcca
     6601 gaaggaattg gtcctgaatt agttgtggga aataatgttt ctttagtcat ccagacaaga
     6661 aatgaagaag aaacgaagcg cctatttgaa agcttagctg agggtggtaa gattgagatg
     6721 ccactggatg ctgttccgtg ggctcagttg tatgggatgg taactgatcg atttggcatc
     6781 aactggcaga ttaaccaaga gtgacagcta aagatgaata ttttctggaa acatcaagca
     6841 gcaaacggat catcgtttgt ttgcttgatg tttttgtgtc tatttcgact atacggtatc
     6901 ttttttttat tgccagatgc gaaaatatgt tccataatag agaagtccgc aaaaaattaa
     6961 gtaacggagg tcagaagatg aagacggtga tactagctga aaaaccttca caagcaaaag
     7021 cctatgccga aaccttccaa cagcaaaagc ggcatgaagg gtattatgaa atcagcgatc
     7081 cattgtttgc tggtgaagta accatcactt atggttttgg acatttggta gatatggttg
     7141 ctccaggagc ttatgaagag cgttgggcaa aatggtcttt agaaaatttg cctattttcc
     7201 cagaaacctt ccgttacgaa gttccaatgg acaaacgtgc acaattttcg atcgtaaaac
     7261 gagaattgca gtcagccgat accatcatta ttgcaacaga tggtgatcga gaaggtgaag
     7321 ccatcgcgtg gtcgattatt cgacaagcaa acgcattttc taaggaaaag aactatttac
     7381 gtttatggat caattctctt gaaaaagaag caatctatga aggttttaag aatctccaac
     7441 ctggaaagaa ctattttcca aaatataaag aagcgcaagc aaggcagaac gctgattggc
     7501 tgattggaat gaatggaagt ccattatata gcttgctact tcaacgaaaa gggattgatg
     7561 gcagtttttc tcttggtcgt gtacaaacac cgacactcta tatgatttat cagctgcaag
     7621 aagaaatcaa acacttcaaa aaagaaacgt actttgaagg agaaggttta attacgacaa
     7681 acaaagggca gttttctgga aaaatcgtac caaaaaaagc atttaaaacc caagaagagt
     7741 taacgaagga aatagaaaaa cttggagcgc atttaggtaa gcaaaacgga cggattcttg
     7801 aagttacgaa aaaggaaaag aggttgcata gtccacgttt attttcgcta tctagtttgc
     7861 agacaaagat gaatcagcta atgaaagcaa gtgccaaagc tacacttgat gctgctcaag
     7921 gattgtacga aagcaagttt ctaagctatc caagaacgga ttccttttac attacagaaa
     7981 atgagcatca gtatctggtt aaacatctgc aaaattataa aaaattttta ggaatagaag
     8041 aagtacagac aactgagatc aaagcaaaga aacgatatgt cgatgcgaaa aaagttcaag
     8101 aacatcatgc aattattccg acaaagacga ttccgactcc tgcacgcttt gcgaagcttt
     8161 ccaaattaca gcaagcaatc tatttgcaag ttttgcgcac tactgtggct atgtttgctg
     8221 cagattatat ctacgaagag acgaccgtaa tgacgggcgt acagcaatta aagctaaaaa
     8281 gcatcggaaa gatcccatta cagcaaggtt ggcaggaaat cttacgcatc aagtcaaaaa
     8341 agaaagaagc tcagactctt cctcctatcg tagaaaatga agaagcagaa gtagaactga
     8401 aaagtatgga gaaagaaaca caacctccaa aaccctatac cgaaggaacg ttgatcacag
     8461 caatgaaaac tgcgggaaaa acgctggata atgatgaagc gcaagaaatt ctcaaagaag
     8521 tagaaggaat agggacagaa gctacacgag ccaatatcat tgaaaatctt aagcagaagc
     8581 attacatcga agtcaataaa aacgaaataa ctgttacgcc aaaaggaatc actctttgta
     8641 aggcagtagc caatgaacca ttactgacga gcgcagaaat gactgcgcgc tgggaagggt
     8701 acttgagaaa aattggtaaa caagagggga caccatctgt ttttttagag aatatcaaaa
     8761 aattcatcct acatttattg gcagaagttc ctaatcaaat cgacaaggta gacttttcag
     8821 atgagattag cgggacacgt cagataaaag cgatggagaa gaaaaacgat caattaggaa
     8881 gatgtccaaa atgtaaaaag ggaatagtca tgctttatcc taaaatcgct acatgcttga
     8941 atccggaatg cgacttcaaa ttgtggccaa cagtagctaa aaagaagctg actaaaacga
     9001 atctcagaga tttgctttca aaaggaaaga caagtaaagt tgtcaaaggt tttactggta
     9061 aaaaagggaa attcgatgca gttttagtat taaaagaaga tatgacaatc ggtttttctt
     9121 ttccatggat cgaaaacagt ggaacaaaag cagttgatgg aaacaaagaa acatcaagaa
     9181 aaaataatga aaacgaagta gagtaaatga aaacaatgaa atagcggaat atttattccg
     9241 aaaagtgaga atataaatac caataaaact aaaaaatatc agtgaatcaa gcaaaaacgt
     9301 tggggtaggc tgattttata tagatagcaa ggagtttttt tatttgaaaa aagtgcattc
     9361 atttttaaat tatcttatgg gagcaatcac gattgtggct gtgtaccaag catttctaaa
     9421 gtatcctgat tacgggacga agttgttgtc attgacgcac aatcaaacag catattcacc
     9481 aacgttgatt ttatcgactt tgactgcagg agctaaaagc gctacttatt tttttcataa
     9541 aacgacaagc atgctaaagc gaaagaaaca gcgaaaacaa ttgttggtgg tggtagcggc
     9601 tatgatcatt atttattttc taccagttat tacacaagta tttcatcaat attttggtta
     9661 agaatacgag agtttgtgac aaaagtcatg tcacaaactt ttttctttgt cattttttcg
     9721 taagaaaaag caaaataaaa gtatatacat tgattaaaag tatatacttg gatataatgt
     9781 acttactata gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa tgaaaaagta
     9841 taaaattaga aaatctggca atagcgatgt aacaactatc ccaccagaag taaaagaatt
     9901 catgggtgtg cagacaggtg acgcaatcag ttatgttttt caatctgatg gttctgtacg
     9961 aatgatcaaa gcacaagaag aacctgatat tgattcatta gttgattcga tcatgaatca
    10021 atatgaagac gcgttaaaag atttggttga tttatgaggt atctttcgac taaagaaatt
    10081 atcaaaatca acgcgaaagt tattttacgt cactcgccag gcgaaatgat cggtgtaaaa
    10141 gatgcaaatg cattagatat ggccgtcaaa cagccttcac aagcagtatt caatcaagaa
    10201 ctctatcctg aaatctatga aaaagcggcg attttagcaa ttaatttagc taaaaaacat
    10261 ccttttcata atggcaataa acgaactgct cttgttgcca tgttgttgtt tcttcaattg
    10321 aataattgtc cagttgcttt ttctcgtcaa gaagctgttg attttatcat aatgattacc
    10381 actagttcat tggatttcga ttcactaaag tccaagataa ccagttattt acgacaaacc
    10441 gcaataaaat gaacgaatta aaaactttat ttctcaatta gatcagcatg tcattaagga
    10501 atatgctga
//
LOCUS       DANJLF010000079        10226 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000079 DANJLF010000000
VERSION     DANJLF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            315..1094
                     /locus_tag="PWT25_002326"
     CDS             315..1094
                     /locus_tag="PWT25_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022372.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1140..2084
                     /locus_tag="PWT25_002327"
     CDS             1140..2084
                     /locus_tag="PWT25_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL1022373.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2100..2879
                     /locus_tag="PWT25_002328"
     CDS             2100..2879
                     /locus_tag="PWT25_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL1022374.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2891..3589
                     /locus_tag="PWT25_002329"
     CDS             2891..3589
                     /locus_tag="PWT25_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL1022375.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3617..4381
                     /locus_tag="PWT25_002330"
     CDS             3617..4381
                     /locus_tag="PWT25_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL1022376.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4425..5876
                     /locus_tag="PWT25_002331"
     CDS             4425..5876
                     /locus_tag="PWT25_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL1022377.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5908..7044
                     /locus_tag="PWT25_002332"
     CDS             5908..7044
                     /locus_tag="PWT25_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL1022378.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7044..8150
                     /locus_tag="PWT25_002333"
     CDS             7044..8150
                     /locus_tag="PWT25_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL1022379.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8156..9178
                     /locus_tag="PWT25_002334"
     CDS             8156..9178
                     /locus_tag="PWT25_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HDL1022380.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9200..>10226
                     /locus_tag="PWT25_002335"
     CDS             9200..>10226
                     /locus_tag="PWT25_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1022381.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNM"
BASE COUNT         3734 a         1440 c         2099 g         2953 t
ORIGIN      
        1 taactattat taattcaatg ttaatttttt tgttattcta ttttttgtgt aacttttaaa
       61 aacacctatg aaaattatgg aaataaaaat gaaatcgatt aatttttatc ttattaatgt
      121 tttatttact tttaaatcgt aaattgccct agtcaagaaa acagtttgtg gtatgattat
      181 atagggaaaa cttgtttaca aatatttctg tgaatatatt agtttaatta tgttttttta
      241 aataaaaagc tgtcattttt atgtaagtga attatatctt aatttatcta tcatcaatac
      301 ttaggagtga atctatggaa aagccattcg tcaagtcatt gactgctgtg gcggtaggtg
      361 caggtgctat tgcgatctgt ttgtttggat accatatcaa taatcagcgg caacatcagc
      421 aacggatcaa ttatgcagaa tctgccatca ccaatcaaaa agacaccttg acttctttat
      481 caaaagaagt agacaagctg tatagtacaa aagaaaaaat ttttctaaac cctgaaataa
      541 cagaagaaac aatcacaagt ttgtcaaata agctatcctc tgtcaaactt tcagcagatg
      601 attacgacat caaagaatca gaattgccta aagaagctgt tgcaatccaa gatgagaaga
      661 aagcaatcgt gacacagtta gatagtgcag aatcaaaact gaagatccag acagctgtca
      721 acgatttatt tactaaaaat gtatcaaact ggcagcaagc tgtagacgat gtcatcatca
      781 aagaaaaggt gacaagtgca gatgtcgctc gtgtacgtga aaatatgagc ttttttaaag
      841 atagtccttg gaaaacggtt gttatgcaat atctaggatt cgctgatatt cagatcgcgc
      901 aagtcacgca actagatcag ttattcgata cgatgttaaa ggatggtcaa gtgactgcca
      961 ccgctactta cgatcaatat ttgacggtgc tcagtcaaat agagcagatc cggaatgaaa
     1021 aaataagcgc agcatacgca acaaaagcac aaactgttgc tgagcaaatg ggctatagca
     1081 atataagcta ttgacagtta aaacgttgat tcaagcaagg ttagcaaacg gaggaaacta
     1141 tggcaagacg aagaagaaaa tcaagaggga aaaaaatatt tctgattatt ttagcagttc
     1201 ttatcgtatt agtattagca attgtaggag taggagcaaa attatatctg gatctctcac
     1261 attctattga gaaaacttac gaatcagtgg aacgaaccca aggagaacga acgaaaccag
     1321 ttgatctgga caaacaagaa tctttctctg ttctattgat ggggattgat actggagatt
     1381 taggtcgggt cgatcaagga agatcagata cgatgatggt tgccacagtc agtcctgaag
     1441 acaaacagac aacgatcgtc agcattcctc gtgatacgta tgtcgacatc gtaggtcgtg
     1501 acacaaaaga caagatcaac cacgcttacg cattcggcgg agctgccatg gcgatggata
     1561 ccgtacaaaa atatgtagac atcccaatcg atcactatgt ctctatcaat atgaaagggc
     1621 taaaggaact cgttgatgca gttggtggta tcgaagtcaa taatgacctc actttctcac
     1681 aagatggcta tgactttcca atcggtaaaa ttacattgaa cggggatcag gcattatctt
     1741 attctcgtat gcgttatgaa gacccaaatg gtgattacgg tagacaaggg cgccaaagaa
     1801 agatcgtaga aggcgtagcg aaaaaagtat tgagtctaaa cggtgtcagc aactatcaat
     1861 ccgttttgaa tgctctagag tcgaatatga aaacagacat gaactttgat gaaatgaaaa
     1921 aaattgcttt agattatcgg gaggcatttg gtacgattaa gcaagatcag ctgcaaggag
     1981 aaggcttcat gcaagataat atctcctatc aaaaagtcag tgatcaagaa cttgatcgtg
     2041 tgaaaaaaga attaaaagat caaatgaatc tagagaataa ataggaaagt ggagaaaaaa
     2101 tggaagagac aatcagcttg aaagaaattt ttgaaacatt acgcaaacac ttggtaataa
     2161 tcatcatctc aatgtttgct ggcttggcca tctcaggaat cgtaacattt ttcatcttga
     2221 caccaaaata cagttcacaa gcacaattga tcgtgacact gccacaatca gacactgcaa
     2281 atgtcaatga tgtcaatacg aacctgcaaa tgatcaatac gtataaagat atgatcgtca
     2341 gtgatttagt gctcaatgaa gtcaaagatc gtttagaaac agaagacaat gtgaaaatga
     2401 ctgctgaaca aatcaaagat gccatctcag ttaatcaggc agaaaattca caaatgttct
     2461 cgattcaggc aataagtacg aatgcagtgg ctgctcaaca gatcgctaac acaacttcac
     2521 aagttttcca agagaacgca aaagatgtga tgaatgttga taaaatctcg atcatttcag
     2581 gtgcagttgc caatacgaca cctgtttcgc caaataataa attgaattta atgactggct
     2641 taatgttagg aatgatggta ggtgtaggtc tggcattctt attggaatta ctcgatcgga
     2701 cagtgaaaga tgaaaaatat gtgacagata cattaggatt cccaatctta ggaacagttc
     2761 cagaaatgtc agcgaaagaa ttgaatgctg gtatccgtaa aaaaggacca gctcagacac
     2821 aaccaaaagc tacaaacgat aaaacagcgc catcacgcag aagccgctca agagtctagg
     2881 aggaaagaaa atggcacgaa cacagaaaca aaaagcagcc caaactcatg cagtcagttt
     2941 ggtcacactc accaatccct cttcaccaat cgctgaacaa tatcgtacga ttcggacgaa
     3001 tatccagttt gcctccgcag ctggtcagca aatcaaaacg attgttgtga cttcatcagg
     3061 acctggagaa gggaaatcga ccactgcagc aaacattgcc gttgtttttg ccaaatctgg
     3121 tcaacgggtg ctgctagtcg atgcagactt acgtaagccc gtcatctata aaacgttcaa
     3181 gttgaacaac gcaagcggat taagtactgt tcttagttca tcaggaagtg tagcagatga
     3241 gatccaacga acaccagtcg acaatttgtc gattctgcca agtggaccta aaccaccaaa
     3301 tccatcagaa ctactaagtt cgccaagaat ggatcagatt ttagcagaag caagacaact
     3361 atttgatgta gtgatcttcg acatgccgcc agtcgttgcc gtaacagatg cacagatcat
     3421 gtcttcaaaa acggatggca ccttattggt cgtacgggaa aatacgtcac gaaaagaatc
     3481 attgactaaa gcaaaagaac tgttagatat ggtacaagca cgagtcttag gtgtcgtata
     3541 caacggagca gaacacagca aagatgctgg ttattactac ggaaattaag gaataagaag
     3601 agaggagaca agagaaatga tcgatctaca ttgccatatc ctaccaggaa tcgatgacgg
     3661 ggcagaaaac ttagaagcaa gtattgcaat ggctgaaaaa gcgattcagc aaggcattac
     3721 gcatatttta tgcacaccac accacaataa tggaaaatac agcaatgaaa aatcacaagt
     3781 catttctcta gtcgcttcgt tacaagccga attagagaaa agacagttgc ctttgacgtt
     3841 attggaagga caagaagtac gtattaccgg aacgctcatc gaagacatcc accgagatga
     3901 aattttattt acagatttag atgatacata tcttctgatc gaatttccga cactggaagt
     3961 gccgttatat gcagaacggc ttttttttga gttgttagaa ctaggaaaga taccagtgat
     4021 cgtccatcca gaacgcaacg cgcattttcg agaagaccca aatcatttgc ttccgttcct
     4081 agatatggga tgtttggccc aacttactgc gccaagttat gttggtgtat ttggcaaaga
     4141 tatccagaaa acagccaaaa caatggtcaa acataatctc gtgcagatgg ctgcttctga
     4201 tgcacacggg gtctctaagc gcacctttta cttaaaagag tgctatgagc agattgccaa
     4261 ggagtttggt aaggaaaaag tagtacagat gaaacaagtc gcaaaggatc tgatcaacgg
     4321 ggatgagatc aattatccaa tctatgaaga ggtaaaaaag aagaagtttg gtttatttta
     4381 gttctttatt cgtagataaa aaggaaatga ggagagagtg agagatgtat agaaaaaact
     4441 caagtggttg gtctaaacat attgatttta ttttattaga cttattttgt gtccagcttg
     4501 ctttttatat atcatatgta atgcgacagg gtgactggaa tccgtatgtg attcctattt
     4561 atcgccacat ggcaattttt atagaacttg agaatcttac gattatattt ttatttgagg
     4621 catataaaaa tgttttaaaa agaggatatt ataaagaggc tgtagcttct ataaagcaat
     4681 cttttatgct gttacttgtg tgcagtttat accttattac aatgcaggat ggaaatgatt
     4741 attccagagt ttctttattt ataatggggg tggtgtatgg attattaaca tatataattc
     4801 gtatactttg gaaagggttg ttgcatcacc gcatgaaaaa cggtgaagat gtatcgttag
     4861 ttattgtaac atctcaaaag attgcaaaaa atgtagttcg taatcttaaa gagaagaatt
     4921 atggcatgta taaacttagt ggtgtgattg tagtagacag agatatgtat ggaactacca
     4981 tcggaggtgt cccagtagtt gcaaatgctg ataatgcagc ggaatatctt ttacagaatt
     5041 gggtagatga agtattcatc aatgttgacg aaagtgttcc atatcccaaa gaattaatcg
     5101 ccagatgttc ggaaatggga cttgctgtac atatgaatct tacaaaagtg tctgatagta
     5161 cacgtggaaa acaatttatt ggccgagttg gtgattatac tgttattaca tctagtatta
     5221 atgttatgac gatgaggcaa gcatttataa agagaacgat tgacattctt gctggaatta
     5281 ttggatgtat tgcaacaggt gttattttta tcttcgtagc accagttatt tacatcaaat
     5341 caccagggcc gattttcttt gcacaagaac gtattggtca aaatggtaag acatttaaga
     5401 tgtataagtt ccgtagtatg tatatggatg cagaagaacg caaagcagag ttgatgaaag
     5461 aaaataagat gagtaataat ctcatgttta agatggattt tgacccaaga atcattggca
     5521 ataaaatact tcctgatgga acaaagaaga caggaattgg acaatttatt cgatctacat
     5581 cattggatga gtttccacaa ttttataatg tcctaatggg aaaaatgagc ctggtggggt
     5641 ttagaccttg cttgaagtca gaatatgaga agtatgattt tcatcatcgt gcgagaattg
     5701 cgatgaaacc tgggattact ggaatgtggc aggtgagtgg tagaagtgat atcacagact
     5761 tcgaagaggt tgtaaaactt gatgcagaat atatcaggaa ttggagtatg ggattagatt
     5821 ttagaatttt gtttaggaca gtcaaaactg tgcttcatag aaacggttcc atgtaagata
     5881 aagatactat agaaggagta aaacgtgatg gcaagaaaaa aattaaaaat ctgtatgcta
     5941 ggtcataaaa gaattccatc aagagaaggt ggaattgaga tagtggtaga agaattggca
     6001 acgcgaatgg ttgcacttgg gcatgaagtg acttgctata atcgaagtgg tcatcatgtt
     6061 agcggaaagg aatttgacca gcataaaaat aaagaatata aaggtgtaaa attaaagact
     6121 atttttacat taaatataaa aggaattgca gcaatgagtt cttcagtatt tggtggaatt
     6181 agagcagcat tcggaaaata tgatgttgtt cattttcatg cagaaggacc atgtgctatg
     6241 ttgtggttgc caaaactttt tgggaaaaga tgtgttgcaa caattcatgg tctagatcat
     6301 caaagagaaa aatggaacaa gcttgctagt acatacatca tgttagggga aaagtgtgct
     6361 gtaaaatttg cagatgaaat aattgtactt agtgaaagcg tccaaaatta ttttgaagat
     6421 atatatggaa gaaaaacaag gtttatacca aatggcgtta aaaaaattga aataaaatca
     6481 gcaggattga ttacagaaaa gtatggatta acaaaagatt cttatatttt gtttttgggg
     6541 cgattggttc ccgaaaaagg aattcggtat ctaattgagg catttaaaga tgttcagaca
     6601 gataaaaaac ttataatagc cggaggaagc tctgatacag atgaatttgc aaatgaacta
     6661 aaggaattag caaaaggtga tgagcgaatt atatttactg gatttgtaca aggacaggaa
     6721 ttagaagaat tatatagtaa tgcgtatatc tatacacttc caagtgattt agaaggaatg
     6781 ccattaagtt tgttagaagc aatgagttat gggaactgtt gtatagtttc aaatatctca
     6841 gagtgtacgg aagtagtaga agacaaggct atgattttta agaaatcgga tgtaagtgat
     6901 ttaaagataa gattagaaga ggcatgcaat caatcggaaa tggttaaagt tttgaagaat
     6961 caagctactg agtttatttg tagtaaatat aattgggata aaatcgtaca agaaacatta
     7021 aatttgtata ggggaaatat ataatgattc gaatattaca gtgtgtgaat gatatgcatc
     7081 gtgcagggtt ggagacgatg ctaatgaact attataggaa tatagatcga actaaaattc
     7141 agtttgattt tttgacacat cgtccatata aaagtgatta tgatgaggaa ataataagtc
     7201 ttggaggaaa ggtctattat gcaccgagac tttatccaca aaattatcct aaatatttta
     7261 aatggatgga acaatttttt aaggatcatc cagaatatga gattgttcat tctcatattg
     7321 atgcgatgag ttatttacct ttaaaagctg caaaaaaagc tgggattccg atacggatag
     7381 cacatagcca taatacatcg atagatagag attttaaata tcttttaaag caatattatc
     7441 gatgtaagtt aacgtctgtt ttgacacatg aattttcatg tggaaaagta gcaggtgatt
     7501 ttttgtttcg aaatgataaa ttcactttga ttccaaatgc agtggatgct aaaaaatttt
     7561 attatgatga aagtattaga aatgatgtac gaatgaaatt aggaatttca agtcagacgt
     7621 ttgtaatggg acatattgga agaatttcat attctaaaaa tcatcgtttt ttgattgaaa
     7681 tttttaaaga atttcacgct attaagaata attccgtatt attaattatt ggaactggtg
     7741 atatggaaga agaaataaaa aattatgcaa agaaaagtgg catagatgat gatattaaat
     7801 ttttaggaaa tagaaatgat atagaaaaat tttatcaagc atttgacgta ttaatgttgc
     7861 catcattatt tgaaggggtt ccgttagtgg gtattgaagc tcaatttgca gatttaccgt
     7921 gtttcttttc ggagaaagtt cctacagaag ttgctttcag tgataagacc aatttcataa
     7981 gtttaaatca atcggcaaaa gaatgggcga gagagattgc aaatgtagat atctctcatc
     8041 gagattcaga acgttcgatt ttaataaagg cagattataa tattcaaaca gcatacaaaa
     8101 tcttagaatc aaaatattat gagttatttg aattaataca aagaggttaa agaatatgac
     8161 acagaaacat tcaagtataa aaagaattat acatcgcata tccgaagaat tgaatgcaac
     8221 aagacgaatt gcaaagataa catcttggaa ggaagcattt gccacgttac aagctaaaat
     8281 tgatattcaa gtcatgaatc gtaatggata taatgaaagt aaaaaacaga aggaacattt
     8341 attaaaaaaa catgaggtaa tgctgaagta ttatgaaaag actttttcag attttttgaa
     8401 aacatatgat tatagtcaaa atcaaataat taatcaatca aaatcaccat attctaactg
     8461 catttgggtt tgttggtggc agggattgga tcaatcgccg gaaattgtaa aagcttgtgt
     8521 aaaatcaata aaacgaaatg cagggaaaca tactgttata attttaacag aagataatta
     8581 tagacaatat gttgatatac caaaatgggt ggaagaaaag aaaaacaaag gaatcattac
     8641 gagaacaaat tattctgatt tattaagatt atctttgcta gcaaaacatg gaggtttgtg
     8701 gttagacgcc acattttatt gtacaaaacc aataccagat gagtattttg atatcgatat
     8761 ttggtctatt aggaggcctg attatgctca cgcatccgta gcatcaggat attttgcggg
     8821 ttattcatta gcatgtaatc aagagtctag atggatgttt gctataatta gagacttttt
     8881 tttaaattat tggaaaaata atgatatgat ggtggattat ttgatgatag attatatgat
     8941 tgttttagca cagagaataa atcatgaaat agcacagaca tttgataaaa tacctagtaa
     9001 caatccatta tgtgacgaat tgattaaaaa tttaaataat atatatgatg aaaaagtatg
     9061 gaacttatta aagaaagata ctttactgtt taagttgaca tggaaacaat cttttaaaaa
     9121 gcaaaaagat gataaagtta ctttttatgg gatgttggta gaaaacaaat tattataaat
     9181 attagaggta taaaaagtta tgaaacacaa ggtaagtgtg atagtaccaa tttacaatgt
     9241 tgaaaaatat ttaagaaaat gtgtggatag tttattgagt cagacattaa aagagataga
     9301 aatcgtatta gttgatgatg gttcgccaga tgcttcgggg gaaatagcag atgagtatca
     9361 aaaaaaatat tctaatgtaa aaacaataca tagagaaaat ggggggttag gaccggcaag
     9421 aaatacagga attgagaatg cgacaggaga atatgtggct tttcttgatt cagatgattg
     9481 ggtagaaagt gatatgtatg aaaaattata cttagttgcg tccaaagaaa atgctgatat
     9541 agtagttagt ggacattgtg attttgcgaa tgacaaagct atggtgaaaa agcaacatcc
     9601 tttgagtggg aatgtatatg atacgcctaa tagtattaaa aatgtacgaa aaaatttatt
     9661 tggacatagt ccaaaagatc aagaagtaga ggccttcccg atgtcagtat gtatgtcttt
     9721 gtataataaa aatctgctag ataaatatag actaagattt gaaaaaattt tatctgaaga
     9781 tacaattttt aacttgaatg catatgatgt cgcaaataaa attgctttta cagaatatac
     9841 agattattgt tatagaaaag aagaacaaga gtctattaca aaaagtttat ctccaaataa
     9901 gcagacggaa tttaaagaat ttttaataac tctaaaagct atagcatcga aagaagattt
     9961 agaatgtcaa atcagagcaa aacgtatggc aattgattat tgtaggcttt atgtgggctt
    10021 agttgatgat gcaaatttgg gaattaaaga gaaaagaaag tatataaaaa aatttattga
    10081 agatgaggga ttaattcaat gttggaaggg atatcctaca aatacgctac catttcaaca
    10141 aaaatttttt cataaaatgc ttgaatgtaa gtgttataca atattgttgt tgttaagtag
    10201 aattagaaag ataattaaaa atatga
//
LOCUS       DANJLF010000080        10185 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000080 DANJLF010000000
VERSION     DANJLF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            122..628
                     /locus_tag="PWT25_002336"
     CDS             122..628
                     /locus_tag="PWT25_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL1022382.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            645..1544
                     /locus_tag="PWT25_002337"
     CDS             645..1544
                     /locus_tag="PWT25_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL1022383.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1631..2505
                     /locus_tag="PWT25_002338"
                     /pseudo
     CDS             1631..2505
                     /locus_tag="PWT25_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(2697..2768)
                     /locus_tag="PWT25_002339"
     tRNA            complement(2697..2768)
                     /locus_tag="PWT25_002339"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2733..2735),aa:Arg,seq:ccg)
     gene            2828..4162
                     /gene="rpoN"
                     /locus_tag="PWT25_002340"
     CDS             2828..4162
                     /gene="rpoN"
                     /locus_tag="PWT25_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL1022384.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4311..5402
                     /locus_tag="PWT25_002341"
     CDS             4311..5402
                     /locus_tag="PWT25_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL1022385.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5443..6444
                     /gene="gap"
                     /locus_tag="PWT25_002342"
     CDS             5443..6444
                     /gene="gap"
                     /locus_tag="PWT25_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL1022386.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6554..7744
                     /locus_tag="PWT25_002343"
     CDS             6554..7744
                     /locus_tag="PWT25_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL1022387.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7848..8603
                     /locus_tag="PWT25_002344"
     CDS             7848..8603
                     /locus_tag="PWT25_002344"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL1022388.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8719..10017
                     /gene="eno"
                     /locus_tag="PWT25_002345"
     CDS             8719..10017
                     /gene="eno"
                     /locus_tag="PWT25_002345"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL1022389.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3561 a         1885 c         2109 g         2630 t
ORIGIN      
        1 atttttgtca caccttcttt tttgtatata aaaatcccaa gtaaatacat aaaaaataaa
       61 aaaattgaaa cttttagcgc accttgtacg tcatattaag tgaggcagaa aggagtggag
      121 aatgattttt actggatata agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa
      181 tggactcatc gaaactgatt atcaaaaaat gttccgtatc acgatgaatt acgtacatga
      241 taaagaagag gccttagatg tgatgcaaga tagttttcat aaagccctga actattttga
      301 aaaagaaaaa gagatcgagc attttagtgc atggttttat cggatattga tccgcacggc
      361 attagatagt tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat
      421 cccccaacag ttacctttag aaacttccgt tgcagagctc catatgattt tagataaaat
      481 agacagtccg gaaaaagaga ttctgatcct ctactttttt gaaggatttc gtttgaaaga
      541 aattgcgact atcttggata tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt
      601 gaatgcttta cgtcaaatcc tagaatgaag gagagaagag tgccatgtat tcccaaaaag
      661 aaaatttaaa aaaactaaag aaaacttata aagaacaagc aattcccaaa gggcttaaag
      721 aggagatccg ccagcgtttt attatcgaag agcaatcatt tataaaagca aagcggagaa
      781 agagacgtct ccaagggttc ggagtatctt tggctatcgt aatgattaca acaagtagcc
      841 ttctttttaa tagtcaagtt cgaagctttg cagaagattt acccatctta ggatcagtca
      901 ttgaattgat cttaggcgaa cgctttactg atcgatctga aaaaatcgat attcaggtac
      961 cgaagatcaa tacacaaaat gagaaagaaa ataaaacaat tcatggatta aatcaaaaat
     1021 actttcgcga aggacaagca gattttgaaa aagctgaaaa agaatatgga aattttgaaa
     1081 cggatcatta ccaagttttg ggagattatc agaaaatcgt agacgataac cggtttctcg
     1141 tgattgaaag gcagatcacc caaacagcag cagatagcca tgttgaaaaa agatacgaca
     1201 caatcgacaa gaaaaacagt gtccagctct cacttccact tttattcaaa gatgacacct
     1261 atctatctgt tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc
     1321 catctaagta ctattggact gagcaagata ttcaagaagg gacgatagaa aaaccaacac
     1381 ttgttacgcc tacacgaagt ttttatttga acaaagagca tcaattagtt ttgacttttt
     1441 ctcaatacga aattgctcct ggttatatgg gtacaccaga atttgtcatt cctaagtccg
     1501 tgacaaaaac gattttggca tcggaagatt atttggatca ttagacgaga agtagttgcg
     1561 actagcagtt tcaattttca agtaagtaca agtaaataat aagaagagaa aaagagaaag
     1621 aagagatata gtgaaaagaa attatttagt tatcagtatt attgctttat ccgtgctttt
     1681 gtttttcgaa attacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc
     1741 ttcggagcat tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc
     1801 aactgaaaca gctaacgtca aagaagccat tcaagaatca gcggaaaagg tacaaagtca
     1861 gtcatcacaa tcaacacaag acagtacaaa aacgagccga gatccggctg tcaaaagcgt
     1921 cgcaatcagc tttgatgatg gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt
     1981 aaaagaaaaa aacgtgcatg ccactttttt cgtcttagga gaaaatacgg cgcagcatcc
     2041 tgaaatcgtg aagcagacag ctgaagcagg gcacgagatt ggcaatcaca catacgatca
     2101 tcagaatctg gccactcttt ctgcccagtc gatgacggag gagcagacac tgagatcaaa
     2161 aaagtcactg gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta
     2221 ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag
     2281 acaagaaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt
     2341 attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat
     2401 ctgaaagagc aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct
     2461 gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct
     2521 ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct
     2581 taacaatatc tatacaggta caacaaaaaa ctgtccacca attttctttg gcggacagtt
     2641 ttcacttaaa aataaagaag caactattcg taaattatgc taaaatagca gtagaacgcg
     2701 tcctgaggga atcgaacccc cgtctcaaga accggaatct tacgtgatat ccactacact
     2761 aaggacgcaa gcactgggat aattgtaact aaacagataa aaaattacaa ggggattgga
     2821 caaaaaaatg aaatttgagc aaaatttttc acaaaaacag cagcagacac agaaactagc
     2881 catgacgcaa cagttgcaac agtccatcca agttttacaa tataacagtg aggagctgtt
     2941 ggcattcgtt gagaatcaag caatggaaaa tccattggtg gaagttgttg agccggaatg
     3001 gcagccagat tacataaaag cttcttcatc ttcttatgaa ggagaagaga caaattacct
     3061 aaatcagatt cctgatacaa aagggtcttt atttgattca ttgatcgaac aagtccattt
     3121 gaattaccga gatacttttt tgaggaaaat cgttctttat cttgtagaat acatcgattt
     3181 gaacggattt ttgacgatta gtttagaaga agccggaaaa gagattggag ctacgcctat
     3241 ccaaatgctc gatgcgttga ctctgattca gcaattagaa ccagctggag taggtgcacg
     3301 tagtttgcaa gaatgtctca tgcttcaaac agaacgagat gattatgcgc cggaattagc
     3361 ttatatcgtg ttggaagaat gttttgagga actggtggag cgaaaatgga aagaaatcgc
     3421 acaacgtttt tctgttgatc tacatgctgt tcagcagata ttcgactatt tacaaacgtt
     3481 gagtccaagc cctggccgga ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt
     3541 ccgggtatta gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc
     3601 aaatatacgc tttcaagaga gttacttcaa acaaatgtca caacaagcag ataaagaaac
     3661 tgaaaattat ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca
     3721 acgtggggat acaattttgc gggttgctca agtcattgtt tcccggcaaa aagctttctt
     3781 tatcgacaag gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa ctgagataga
     3841 tgtgcatgaa tccacggtca gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg
     3901 tgtatttgaa ttaaaaaaat ttttcactac acgaattcct acgaatagta cagaacagac
     3961 agaagacctt tcagcagata cagcgaagaa aaagctgcag gaattagtcg atcaagaaga
     4021 caaaaacaag ccgttgtctg accagaaatt ggttgaattg ctgaaaaaag atgagatagc
     4081 gatctcaaga cgaacagtag caaaatatcg tgatttatta ggaatcccga gttcatcaaa
     4141 aagaaaacgt tatgataact aatcagtcaa aagactagaa aaagtatttc actttttcta
     4201 gtctttttag ttgaaaaacg agagcaaaac tgttagactt acagtcgtga ggttgagatt
     4261 tccctttatt gatgtgaaat tcgaatagtt aatttctttt tttatcgtat atgggtcgtt
     4321 ttaagtctac attggacgta aagtgtccaa ctaaggagat cgctatgctg gatgaaataa
     4381 aaatgattga atctgttgca ccagatatta tcgaagtgtt gcaagaacgc tataaaattt
     4441 tacgaaatat ctattggatg cagccgattg gacgtcgaag tttatcggaa agtatgggga
     4501 tcacagaacg agtattgcga acagaaacag atcttttgaa aaatctgaat ttgatcgaca
     4561 cctcaaaaag cggtatgacg ctgacaacta agggcgaaga agtttatcaa agccttgaaa
     4621 acttcatgga tcagctttta ggtacgcatc aaaccgaaca acagttggca gaatattttg
     4681 gtaaccaacg ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg
     4741 cattcggcga agcgctgagc gaagccctag accgactact tccagaaggc gagaatatca
     4801 tcgctgtgat gggaggaact actatggctg tggtagccga acaattgtct aatctggaaa
     4861 ataagaaacg gcataacctg tttgtccctg caagaggcgg gattggtgaa gcgataactg
     4921 tacaggcaaa ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac caccgtgcac
     4981 tttatgttcc ggaacaatta agtctggcaa cttacaattc tttactcaat gagccatcta
     5041 tccaagaagt cttaaacttg attagtgaag cgaattgtgt tattcatagt attgggcgtg
     5101 cattacacat ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag
     5161 caaatgctgt tgcggaatct tttggctact ttttcaatga aaaaggtgaa gtcgtttata
     5221 aggtccctag aatcggcata gaattgcaag atttggaaaa agtcccgatc atcatggcaa
     5281 tcgcgggcgg aaaatcaaaa gccaaagcaa ttcgagctta tatgaaaaat gcaccaaaac
     5341 aaacgtggct catcactgat gaagctgctg caaatgagat tttaaaaggg gtaacccttt
     5401 aaaataaata tttttttgat tttcataagg aggaaatctt aaatgacagt taaagtaggt
     5461 attaatggtt ttggacgtat tggacgtcta gcattccgtc gtatccaaga tgtagatgga
     5521 atcgaagtag tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa
     5581 tacgacacaa ctcaaggacg tttcaacgga acagttgaag ttcatgaagg ttcattcaac
     5641 gtaaacggaa aagaagttaa agtattagct aaccgcaacc cagaagaatt accatggggc
     5701 gaactaggcg tagatatcgt tctagaatgt actggtttct tcacttctaa atcagcagct
     5761 gaaaaacatt taacagctgg tgctaaacgc gttgttatct ctgctcctgg cggaaacgat
     5821 gttccaacaa tcgtttataa cacaaaccac gaaacattaa ctggtaaaga aacagttatc
     5881 tcaggtgctt catgtactac taactgttta gctcctatgg ctaaaacatt gaacgacaaa
     5941 tttggtgttg ttgaaggatt aatgacaact atccacgctt acacaggtga ccaaatgact
     6001 ctagacggac ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc
     6061 gttcctaact caacaggtgc tgctaaagct atcggtttag taattcctga attgaacggt
     6121 aaattagacg gagctgctca acgtgttcct gtaccaacag gttcattaac agaattagta
     6181 acagttcttg aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa agaagcttca
     6241 aacgaatctt acggatacaa cacagacgaa atcgtttctt cagatatcgt tggtatgact
     6301 tacggttcat tatttgatgc tacacaaact aaagtaatga cagttggcga caaacaatta
     6361 gttaaaactg ttgcttggta cgacaacgaa atgtcataca ctgcacaatt agtacgtact
     6421 ttagaatact tcgctaactt ataagataga caaacgtaca agtgtaaatt atagacatgt
     6481 acatttaaaa gcggggaagc aatcgcgctt cttccgcttt ttttgataat aagtttattc
     6541 aggaggaaat aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt
     6601 tcttgtccgt gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg
     6661 tatcaaagca gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct
     6721 tttctctcac cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc
     6781 agttgcaaaa cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg
     6841 cggagaacaa ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga
     6901 aaatacacga tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg
     6961 taaatactgg gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg
     7021 tgcacatgct tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt
     7081 gatggaaaaa gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat
     7141 tgcaatcctt ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc
     7201 taaagcagat aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg
     7261 aatcaagatc ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat
     7321 cgaaaaagca ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc
     7381 aaatgatgtt gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga
     7441 catcggccca gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt
     7501 ttggaacgga cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt
     7561 ttgtgaagca atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc
     7621 cgcagcagca gaacaattag gatttgctga caaattcaca cacatctcaa caggtggcgg
     7681 cgcaagcttg gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa
     7741 ataaacgacc aagtttgctc aattctgacg agtaatcgat caaaaggtga agggagtttt
     7801 catgactccc tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta
     7861 ttatcgcagg taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag
     7921 cagtaaaaaa caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat
     7981 tattcttaca agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa
     8041 actgctactg ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg
     8101 atctaggtgt tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa
     8161 cagacgaaga catcaataaa aaagcgaaag ctatctttgc aaatggtatg acaccaatcc
     8221 tatgctgtgg cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag
     8281 gacaaattac agctggttta gctgatcttt cagctgaaca agtgtcaaac atggttattg
     8341 cttatgaacc aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg
     8401 aaatttgtgg agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag
     8461 cagttcgtat ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa
     8521 aagaaaatgt tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt
     8581 cattgttaga cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc
     8641 actaatgagc aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac
     8701 tcaaaggaga gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac
     8761 tcacgtggta acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc
     8821 ggaatggttc cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga
     8881 gacaaagctc gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc
     8941 attgctgaag caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg
     9001 atcgctttag acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt
     9061 tctatcgctg ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta
     9121 ggcggattca acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca
     9181 catgctgata actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca
     9241 ttcaaagaag ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa
     9301 gctcgcggat tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca
     9361 aacgaagaag gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt
     9421 aaagacgttg ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt
     9481 gtttacgtac tagctgattc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc
     9541 tacgaagaat tagtttctaa atatccaatc atctcaatcg aagacggatt agacgaaaac
     9601 gactgggacg gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt
     9661 gacgacttgt tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct
     9721 aactcaatcc ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc
     9781 gaaatggcta aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa
     9841 gattcaacaa tctctgatat cgctgttgca acaaacgctg gccaaatcaa aactggttct
     9901 ctatcacgta ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt
     9961 ggtgaagttg cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc
    10021 ttttctaatg aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta
    10081 agtaatcgaa ctctctactt gctgaaatat cggctctttg tcaataagga ctgatgagtt
    10141 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DANJLF010000081        10086 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000081 DANJLF010000000
VERSION     DANJLF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(247..759)
                     /locus_tag="PWT25_002346"
     CDS             complement(247..759)
                     /locus_tag="PWT25_002346"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL1022390.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="PWT25_002347"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="PWT25_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL1022391.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="PWT25_002348"
     CDS             complement(3212..3655)
                     /locus_tag="PWT25_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL1022392.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="PWT25_002349"
     CDS             complement(3656..4444)
                     /locus_tag="PWT25_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1022393.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="PWT25_002350"
     CDS             complement(4441..5382)
                     /locus_tag="PWT25_002350"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL1022394.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="PWT25_002351"
     CDS             complement(5559..6146)
                     /locus_tag="PWT25_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL1022395.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="PWT25_002352"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="PWT25_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL1022396.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="PWT25_002353"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="PWT25_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL1022397.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="PWT25_002354"
     CDS             complement(9063..9998)
                     /locus_tag="PWT25_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1022398.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DANJLF010000082        10084 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000082 DANJLF010000000
VERSION     DANJLF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            162..392
                     /locus_tag="PWT25_002355"
     CDS             162..392
                     /locus_tag="PWT25_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL1022399.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="PWT25_002356"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="PWT25_002356"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL1022400.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="PWT25_002357"
     CDS             1803..1979
                     /locus_tag="PWT25_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL1022401.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="PWT25_002358"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="PWT25_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL1022402.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="PWT25_002359"
     CDS             2474..2941
                     /locus_tag="PWT25_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1022403.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="PWT25_002360"
     CDS             3026..3910
                     /locus_tag="PWT25_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1022404.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="PWT25_002361"
     CDS             4024..4260
                     /locus_tag="PWT25_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL1022405.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="PWT25_002362"
     tRNA            4348..4428
                     /locus_tag="PWT25_002362"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="PWT25_002363"
     tRNA            4435..4506
                     /locus_tag="PWT25_002363"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="PWT25_002364"
     CDS             4631..4801
                     /locus_tag="PWT25_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022406.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="PWT25_002365"
     CDS             5021..5728
                     /locus_tag="PWT25_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1022407.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="PWT25_002366"
     CDS             complement(5788..6432)
                     /locus_tag="PWT25_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL1022408.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="PWT25_002367"
     CDS             6559..6846
                     /locus_tag="PWT25_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022409.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="PWT25_002368"
     CDS             6954..7274
                     /locus_tag="PWT25_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1022410.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="PWT25_002369"
     CDS             7355..8668
                     /locus_tag="PWT25_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL1022411.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="PWT25_002370"
     CDS             8665..9384
                     /locus_tag="PWT25_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL1022412.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="PWT25_002371"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="PWT25_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL1022413.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DANJLF010000083        10063 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000083 DANJLF010000000
VERSION     DANJLF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(526..750)
                     /locus_tag="PWT25_002372"
     CDS             complement(526..750)
                     /locus_tag="PWT25_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022414.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            941..3718
                     /locus_tag="PWT25_002373"
     CDS             941..3718
                     /locus_tag="PWT25_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1022415.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3799..4218
                     /locus_tag="PWT25_002374"
     CDS             3799..4218
                     /locus_tag="PWT25_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL1022416.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4230..4700
                     /locus_tag="PWT25_002375"
     CDS             4230..4700
                     /locus_tag="PWT25_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1022417.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4717..5496
                     /locus_tag="PWT25_002376"
     CDS             4717..5496
                     /locus_tag="PWT25_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1022418.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5483..6304
                     /locus_tag="PWT25_002377"
     CDS             5483..6304
                     /locus_tag="PWT25_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1022419.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6321..7370
                     /locus_tag="PWT25_002378"
     CDS             6321..7370
                     /locus_tag="PWT25_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1022420.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7383..8387
                     /locus_tag="PWT25_002379"
     CDS             7383..8387
                     /locus_tag="PWT25_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1022421.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9020..10039
                     /locus_tag="PWT25_002380"
     CDS             9020..10039
                     /locus_tag="PWT25_002380"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL1022422.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3375 a         1656 c         2098 g         2934 t
ORIGIN      
        1 aagtaaatgt ttatagatga aactctattt acttcttttt taatttgttc ttgcaatctt
       61 ttttgctacg taatttttgt aaaaaagtgg gttgagagaa atctaattga ttttcaaaga
      121 aggagtatga aaatgagaaa aatatttatt cgtttattgt gggtactatc ttagaggaat
      181 aaataatagt ggagaaaact atggtttggc gcagattcta tcaggtttga tggaacgaag
      241 acaacctccc gacatcaagt caaaagctac ttgagatttt gatctgaaaa taaaaaaaga
      301 aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt aatcaatggg aaaaaactaa
      361 gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag tacctcttta ctctctgaaa
      421 gcatcataac tcgtttttcc tcattatgcc aataaattgt tgttatacca catataagga
      481 tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt gaatcttatt ttgtatggaa
      541 ggatttatag aacgcaaaaa tcaagataaa gatgaataaa aatcctgttt cggctatata
      601 ttgcgtatct acgccaataa aacgagtttg ttctgtcata gccatataaa gcaaaggcaa
      661 aaatacaact acagcgagat aagatccgat gatttctcga aaagttttcc aggaagtatg
      721 tttcctgttt ttatatttat ccgatttcat tgaatagtca tagcctcctt attctttcat
      781 atgttgaaat tccagtataa aaactggaag gaactgtacg ttttttagaa taaaaattat
      841 aagggataag taggctgctg tctataataa gatatatttt tcaattcttt attaaagatt
      901 ttattgacaa aatgaatccg ttttctatga tggaggaggg gtgaataagt tgttgaagaa
      961 ggaaatttta tgttacttag agaaccagac agcctttttc gatttagaac atatgaacga
     1021 ggtctttaca gcacaaaggt tagctgatca ttttggggta aaaagaaaca cagtcagtca
     1081 ttatttgaac cagttaaatg atgagcaact actagtcaaa atcaattctc gaccagtgat
     1141 ttatttccac aaagaagctt ttgaaaaaca attttttaag ttacgcacga atttttattt
     1201 ttctatcgat gatctcaaag aggaacagcc ttttttcgcg caaccaaaag acttattctc
     1261 ttttatgatc ggatacgacg ccagtttgaa agaaagtatc gaacaaatca aaacagctct
     1321 ttactatcca gatggcggat taccactttt gattacaggc gaaagtggca caggtaaaag
     1381 ttatttagtt aatttggtct atcagtattg tctgcttcat gatctgctag aagattctgc
     1441 accattcatc acttttaatt gtgcacaata tgcagataat cctgaacttt tgactagcaa
     1501 tttgtttgga catgttaagg gagcgtacac tggtgcagaa gaaaataaaa aaggggcttt
     1561 tgaagcagca gatggtggga tcttgttttt agacgaggta catcgcttaa caccagaagg
     1621 tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt tatcgtatgg gagaacctaa
     1681 tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg actgaagaga ttactagtca
     1741 tttcttaaca actttcattc gaagaatacc tatccgaatc gaactacctg cacttaatca
     1801 gcgtagtcgt aatgaacggc tagaactgat ttactctttt tttctcgaag aacaaagaaa
     1861 aatggggagc cctttgtcag tcagtggacc agctttgtca cttttaacaa gtcagaaatt
     1921 atccggaaac ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa aagcatatgc
     1981 tgaacagcgt gaacagcctg aaatccatgt cacgatccat catttgcaaa aagaactact
     2041 tgcgcagcct attactaaaa atacagcaca gcaaggaatc tatattacgc aagaccagac
     2101 cttagagcaa ttaatggaga agcaacagcc cgaacagcag cgaatcgtta aaagttttga
     2161 gcaaatccta attgatttca agaaaaataa ctgtctgcta tattcttgcg aaggaaaatt
     2221 aaagcaagaa gtgattcaat tatttgattt tttgctgttt gagaccagta gacaagaaaa
     2281 acacgagctg ctggtttatt ggacacaatc tatccgagag acctttcgac aaatggaaac
     2341 tgcctatcaa atcaaattcg atggaaacag tgtctatgcc ttgagttact atctctatca
     2401 gcgaagcact atcaaatggt taccggaaga acatgatatt atccaactaa tcaaagaatt
     2461 agaacaacaa gtttctcagt cttatccatc tagttatcac tatgtccaac gaattctgga
     2521 attaagtaag atcaaattag atattgaaga cacaagtatg gatcggattt tactgacgat
     2581 ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga tgattgctgc
     2641 tcacggttat gcaactgcta gcagtatggc aaatgtagta aatcgtttat tacaagagga
     2701 tctatttgaa tcatttgata tgccactaga tgtcacacca cagcagattg cttcagaaat
     2761 ccttgattat tgcgaaaaca gcgatgtttc aaatggattg atcatattag tagacatggg
     2821 atctctgaaa gaaatccatc agttttttaa aaaacagtta tccgtaccgc ttttgatttt
     2881 gaataatgtg acgacacctt tagcaatcac agtaggggaa tgcctgcaaa aaaatgtatc
     2941 attagaagag atagcagaag agactgttcg acagatccag ccagaatggc gactgctcta
     3001 tccagaggaa aacaaaccca aagcactcat tactacttgc ttcactggaa tcggtacggc
     3061 ggtccactta agtgaactgc tagaaaaaag cttacctacg gcttgtcagt tgaaaatcat
     3121 cccatatgaa tatcagcaat taaaagataa gaaaaacagt gacccattat tttccatata
     3181 cgaagtcgta ggaatgattg gcaccacaga tcctgatatc accaatatcg actatctttc
     3241 attagaagac ctcatctcgg gggaaaagat gtctgtacta gctgaatggt tggacactac
     3301 tatgaatgct tcagagaagg aaatattcaa tcagcgcatc attcgtaatt tttcattgga
     3361 aaaagtactg gattccgtga ctattctaga tacagagaag gtgatgggag aaatcgatca
     3421 ttttatgaga gaattagagg tgcagttgaa tcgaagcatc agcaatccca aaaaattagc
     3481 gttgtatgtc catgtgagct gtctcattga acgattgatc cgacaaatgc caatcgaaac
     3541 ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg ctattaaatc
     3601 tgcgtttagt gtcattgaag agcattatag tgtcatgatc cccaattctg agattgccta
     3661 tatctatgat attttgagtg aagatacaga gtttctctcg atagaagaag aattttgaac
     3721 ataagcaagt tgttgccact aattttggca tgcaacttgc ttttttagta tgtgtaagtg
     3781 aaaagaggag atgaaaaagt ggatagagaa attatcttag cttcccatgg aaaatttgct
     3841 tcagggatat tagattcatt agagctgatc tatgggaaaa atcattctat tactgtattc
     3901 gattgctata ctgatgagaa ttttgattta gcggaaacag taaggcagct ttttatgagg
     3961 tacaaagata aagaaatcat tgttctgacg gatctttttg gcggaagtgt caacaacgaa
     4021 ttcttacaat atatcaatcg ggatcatgtc tatctcgttg ccggattgaa tttgccacta
     4081 ttgattgagc tagtatctcg gttggacgct gaagtagaga caactgtttt gatccagcag
     4141 gtgctaactg attcaaaaaa aatgatccag ttttgtaacg agcggctcaa tcagaaaata
     4201 gaagaagacg aattttagga ggaagaaaaa tgattgtatt gacaagagtc gaccatcggt
     4261 tattacacgg acaagtagct ttttcatgga cacaaacaat aggcgcagac tgtattttga
     4321 ttgctaatga tgatgtacct acgaatgaga tacgaaagac aacgatcaaa ttagccaagc
     4381 cacaaggagt caaactagtg atcaaatcaa ttgatgactc gattgccgcg ttgaaaagcg
     4441 gggtgacaga taaatataag ttattcatcg tagtggaatc gatcgaggat gcatataagc
     4501 tggcagaagc gtatccagaa atcaaaacca tcaacttagg agggatcaag gcaaaacctg
     4561 gtactcgcag catcggaaaa gcggtaaata ttttaccaga agaggaggaa cgcttgaaac
     4621 gattaattga caaacaaata gaagtagaaa tccgtcaagt accagcagat aaaaaagtgg
     4681 atgtaagaga agttttgtaa gaaaggaaga acctcaatga ttgtacaagc agtcttatta
     4741 ggaattgttg catttgtcgc acaatcagaa tatgcactag ggacatcgtt gctttctcgt
     4801 ccgattgtga cggggctgtt tacaggaatc gtgttaggtg atgtaaaaac tgggatcatt
     4861 atgggcgcaa ctttagagtt agcatttatc ggatcgttct cagtaggtgc ttcgattcca
     4921 cctgatgttg tgaccggtgg gattttaggg acggcttttg ctattacagc tggagccggt
     4981 acagaaaccg cgcttttact aggtttgccg attgctacgt tgacattgat attgaaaaat
     5041 atttacttag ggcttttgat tccaattatg aaccataaag cagacaatta tgcagaagaa
     5101 ggaaattata aaggagtcga gcatatgcac ttgcttgccg gtatcggtct ttccttgatg
     5161 cttggtgttg tggtaatggt ttcttatatg gtgggaagca atacgatcgg aaatctatta
     5221 aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag ctacagggtt gattccagct
     5281 cttggttttg cgatgcttgc tcgtttgctg atcaacaaac aagttgcacc atattttttc
     5341 ttaggttttg ctatagcttc ttacttagag attcctgtga ccggagtagc tatttttggt
     5401 gcgattttag cagttgtggt agtcaatatt atgaatgtcc gtcaactacc acttcaaaca
     5461 actgcagggg aggtagaaga agatgaagaa ttctgagatc aaaaacagca aaataacgaa
     5521 aaaagagcta aatcacgtat tttggcgttc ttttcaaatg gagttttctt ggaattatga
     5581 acgtcagatg aatatggctt atgtttatgc aatgattccg attttgaaaa agttatatca
     5641 gtcgaaagaa gaaatggcag cagctttgaa acggcatcta gagtttttta acacgacccc
     5701 acatatcgtt acactgattt taggaatcac ggctgcaatg gaggaagaaa ataaggatga
     5761 tcctgaattt gatgtgactg cgatcgatag tatcaaaaca tctttgatgg gacctcttgc
     5821 agggttagga gattctttct tttggggaac tttgcgattg attgccacag gtgtgggtac
     5881 ctctttagcc atgcaaggaa atattttagg ccctatcttg ttcattttga ttttcaatat
     5941 tccgcacatt ctgtttcgct atctagcaac aggttgggga tatcgcttag gaacgggttt
     6001 tttgaaaaag atccaggcaa acggtatgat ggaaagtttg acattaggcg cgtctattat
     6061 tggactgatg gtcgttggag caatgactgc cacgatgatc accatcaata ttcctttaaa
     6121 aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt tttgatgata tcgttccaaa
     6181 tatcttatgt cttggagcat ttggcatagt gttttatcta ttgaagaaag aagtgaaacc
     6241 attaacgatc ttgatcggac tagctttctt tggaattttt ggttcgtgga tagggatttt
     6301 ttaaaaaagg agcagagaaa atgtttacga tgcaagatta tatctatgaa gaaaaggaag
     6361 tactaagtac gatcttgaaa aagaatgatt tttcgacaag agaacatatg aaaaaaacag
     6421 tgaatttact gatcttagca acgggttcat cttataacgc ttgtttagca gcgaagccag
     6481 cattggaatc ttatggtgat cttactgtcg atattcaaga acctttttat tttttcaatt
     6541 atggaaaact ttctccttca atcgatactg tgattgctgt gtcacaaagc gggaaaagtg
     6601 cttctactgt tgaagcagta aaaatgatac aaaaacaagg gttacctgtt gttgctatca
     6661 caaatgacgt gcaaagtcca cttgctttag aagctgcaca aattattgat ttaggtgccg
     6721 gggtagaatc agtaggcttc gttacaaaag gctattctgc gactgtttta cagttactac
     6781 tcttagggat aggaattgga ataagtaaaa ataaaatcag caaaaaaata gaacaagact
     6841 acatgcaaca gttgcgcaaa ataatcaacc atttaccagc gattattcaa aaaacggaag
     6901 aattttttga tggatatcaa agtttatttc gtttagcaca gcgtttcgtc accattggat
     6961 atggtccaaa ctggggaaca gctaaagaag cagagacgaa attgactgaa acaatccgcg
     7021 ttccgtcaca aggatttgag ttagaagctt atatgcacgg cccatacctg gaggcagatg
     7081 cttcgcattt gctattcttt attgaagggg aaagtgtaaa taaggaacgt tcgcaaaagt
     7141 tgcaaagata tatgagtcgg tacgttggag agactctcac cattaccaca aaaaaagcaa
     7201 gaaacgagaa aacacttgga ttagcaatag aatgtgatga atatctgtct gtattagcgc
     7261 ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt agctaaaggt attgacttga
     7321 acaaaaagat ttttgaagac tttgatacag tcttgaaaag taaattataa gaggtggaaa
     7381 tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat aaaaggcgca ctagctttac
     7441 gtccgcaagt agaagaaatc atcgataaac tttatgcgga aaaatttgac gctgtctatt
     7501 atctaggaat cggtggaacg tatgcttcct cgatgcaagc tgtcacttat atgaatggga
     7561 aaagcaactt gcctgtcttt gtacagcatg ctgcagaata ttacactact ggaaacaaaa
     7621 gattaacaaa agattctatc gttattttgt cctctgttac cggtacaact caagaagtag
     7681 tgaaagcagt agaacaaatc aagaaagtag gagcgacact gattggattt atcgataaag
     7741 cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac ttatccagct cctggaacag
     7801 aacaaatcaa attttttatg gcagcagatc gattgatgta tttgaatgga gaattcgaag
     7861 cttattcaga atactatgaa cagctcgaac gttatttacc aacagggtta gtggaagcag
     7921 agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa acatcgacgt gatagtatgc
     7981 attattttat tggagcaggg aatcaatggg gagctgttta ttcatatgcc atgtgttact
     8041 gggaagaaca aagctggctt ccatcgaaat ctatccatgc tgccgagttt cttcacggga
     8101 cgttagagat cgtagaagaa acaaccccag tcacattatt tttaggcgaa gatgaacaga
     8161 gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat ttgcagcaat tatacattga
     8221 tagacacaaa agactatcca gtagaaggta tcagtgaaaa atatcgcgga cgtgttttgt
     8281 ctttcttatt gatgcatgta gtgacacaac gcatcgatgc tcatgtggag cagttgaact
     8341 gccatccatt agagatacgc cgctactatc gtcagtttga ttattaggat agactggata
     8401 aaaagaatat aaaaaataaa gagagcagcg ataactgctc cctttatttt tttaaaatat
     8461 tggcatcaat tacgttataa aacacatttt ttgcattatc tacagttcaa cttataatct
     8521 gtttaatggg acatgtgggt gccaaccatt ttttgttatg caatagtact atttagttgt
     8581 tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc gccagtttac atagcgtttt
     8641 gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa ccgtcaacag agacaatctg
     8701 tccgttgcag gacctgacag atttagaaat gttcaatctg tctcttatgt attttaaatc
     8761 aatactcact aggagaagtt tcctaacctc ctaatagtaa tgctggtagt aacattgtca
     8821 aaactgcttc ctcttaggaa caagcaaagc ataacgagtg taaaaataaa atgtagacta
     8881 ttgtaacata tcattgatta tttcaagtaa aaacgataaa agaagtaaac aaacacttaa
     8941 aaacaatgga aattagtccc aactttaaga aatgtatact ataatggaaa ttatcaatta
     9001 tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt cttggtcctg gatcttgggg
     9061 cactgcttta gcacaaacat tggcagaaaa cgggcatgat gtccgtattt ggggaaatgt
     9121 acctgaacag attgatgaaa tcaataagta tcatacaaat cagcattttt tacctgattt
     9181 gacgatccca gaatcgatta tcggatataa agaattggca gaagcagtgg ataacgcaga
     9241 tgctatttta tttgttgttc caacaaaagc gattcgttca gtcgcacagg aacttattgg
     9301 taaaatgaat actaagccaa tcattataca tgcaagtaaa ggactagaac aagatacgca
     9361 taaacgaatc tcagaagttt tggctgaaga aattccagaa gaaaaaagac aggcaattgt
     9421 tgtattatct ggtcctagcc atgctgagga agtggcagta catgatatca caacaattac
     9481 agctgcaagt attgatcaaa aagcagcagc ctatgtacaa aaactgttca tgaatgatta
     9541 tttcaggatt tatacaaata atgatgtgat tggcgtagaa actggtgcag cactgaaaaa
     9601 tatcattgca attggtgccg gcgctatcca tggattagga ttcggagatg atgcaaaagc
     9661 agcaatcatg actcgaggat tggcagaaat cagccgtctt ggtgtcgcga tgggtgccaa
     9721 tcctctgaca tttatcggtt tgagtggtgt gggggacttg atcgttactt gtacaagtgt
     9781 tcattctcgc aattggcgtg ccggtaactt gttaggacag ggacataagc tggaagaagt
     9841 tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg acaaaagcag cagtagagct
     9901 agctcaacag ctaggtgttg agatgccaat tactcaaaca atctataatg ttttgtataa
     9961 tggagaagat atcaaaaaag cagcaaaaga aatcatgtta cgtgacggaa aaatggagaa
    10021 tgaattttct cttcgttaaa tagatgatca atagttaatc gtc
//
LOCUS       DANJLF010000084        10047 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000084 DANJLF010000000
VERSION     DANJLF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(803..1189)
                     /locus_tag="PWT25_002381"
     CDS             complement(803..1189)
                     /locus_tag="PWT25_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL1022423.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(1186..1707)
                     /locus_tag="PWT25_002382"
     CDS             complement(1186..1707)
                     /locus_tag="PWT25_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL1022424.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(1709..2743)
                     /gene="sppA"
                     /locus_tag="PWT25_002383"
     CDS             complement(1709..2743)
                     /gene="sppA"
                     /locus_tag="PWT25_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL1022425.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(2776..4191)
                     /locus_tag="PWT25_002384"
     CDS             complement(2776..4191)
                     /locus_tag="PWT25_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDL1022426.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(4160..6313)
                     /locus_tag="PWT25_002385"
     CDS             complement(4160..6313)
                     /locus_tag="PWT25_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDL1022427.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            6571..6807
                     /locus_tag="PWT25_002386"
     CDS             6571..6807
                     /locus_tag="PWT25_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDL1022428.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            6826..7041
                     /locus_tag="PWT25_002387"
     CDS             6826..7041
                     /locus_tag="PWT25_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDL1022429.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(7744..8496)
                     /locus_tag="PWT25_002388"
     CDS             complement(7744..8496)
                     /locus_tag="PWT25_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1022430.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(8511..9758)
                     /locus_tag="PWT25_002389"
     CDS             complement(8511..9758)
                     /locus_tag="PWT25_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL1022431.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(9834..9980)
                     /locus_tag="PWT25_002390"
     CDS             complement(9834..9980)
                     /locus_tag="PWT25_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL1022432.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT         3295 a         1763 c         1400 g         3589 t
ORIGIN      
        1 ataatatatt tcctataatg taaaaagcgt cgatacttat taaagcatcg acgttatttt
       61 tatagtaaat ttatcaaaat ttttgccaac attttttccg atttttttcc ctatttctat
      121 ctttccttat ctttcttagt taatattatt tgatgttaat atatcactac taatattaaa
      181 aaacattgat ttatcatagg ataaagagtg atagtttaat acttaaagag acatattaat
      241 tttatttaaa aattgacatt aatgactctt aatcagcggg tcgcgggttc gagcccctca
      301 cggcccattg ggtgccaaac ccacggaaca ggtaaatgtt ggtgcgaaag catttcgaaa
      361 acatttacgc tgttcttttt ttatttttca aaataactag ggctttttat ttttaactca
      421 aagtccactg tagccctttg gactttgagt taaaaaagaa gcataattat gaacagtaaa
      481 gtttctaata taacaataat tcgagaagct ttgcaaaatc atgctgccaa tgaactctct
      541 aaacatatag gattaaatct aagtactata aaaggttaaa aagtgaagaa tatttaatag
      601 aaaaactaaa tttacttgct gctttcccct tttccattaa tacaacaatc agagagtgtc
      661 tttttttcga aaaaattaga ataaataaaa gacacgtagt ttttttatct acatgtcttc
      721 catcgactac aagttatcat ttctcaaaac gtatttagct tttaattgat taatcgtttc
      781 acgttgtcta cacattgcct aattaagatt ttcttattag aagcatcaat cccgcaataa
      841 tacctaaaac tccaggaatt acccacaata atgttaaaag aaaaaatgga ataatagata
      901 aaatgagtaa actaatacca tagaccactg ttttttgatt ttttcttact aaaaaaccta
      961 ttatgaaaat cattatcgaa aaaactaggg taattcctcc aaataaaaca acattcccgc
     1021 ttagattgac taacatatct atatccttac tggttagttc tgtagtattt aaagtagttg
     1081 aatcaataga agatagaaac ataccaccca tcaatgctaa aagactacat aaaatccctg
     1141 ttattccact aattaaaatc aaaataaact ctgtttttct cttattcatt taattctctc
     1201 cagattaagt gttttagaat tagaaatttt ttgaccatag ttaatagctt ctaatgaacg
     1261 aataacaacc actgacgtgt tagaaaaata atccccaata tgttgtttct tgggcgtgaa
     1321 agcagccacc aaatacccaa tttgaaatac aaaatttttt aatataaacc tacatataca
     1381 ctcacgaata aagatagttt gccaacttag ctctttttcc tcaaagctca ctactcttat
     1441 cccaaaaatc attttcccaa ttgtttgtcc tgaattcagc ttagtcaaaa gtacaaaata
     1501 ggttaaataa atagctaatg acgctatacc ataaaaagta ataatggatt tgtcagattc
     1561 cagtcccatg attttgtaaa agagacctat agtagcatgg gtgatgatgc taatacaaat
     1621 taaatcaata agaaaagcaa aaaatcgtaa ccaaaatccc gcaaatacat aattaggcaa
     1681 agtttcaatg ccactcttct tattcatttt atagccctcc atagtaatac attggttttg
     1741 gagtatcaga aaatccaagt ttttcaaaaa ctgaaattac acgatctgat gtagttgcct
     1801 ttagcccttg ccattcagca atcttattac ctaaccaaga agaagcaaag ccagttgtgt
     1861 catttttata ttctataacc gtcgcatcag ttaatttctt ctcttttttt agggaatcta
     1921 atgcatcttc agagtaacca atttgatcca ccaacccatt ttctactgct tggttaccat
     1981 cataaattct tccatccgca agttctttca ctgcattagt atccatattt cttccttttg
     2041 ctacaacatt gacaaaacga tcataagcac tcatcacaaa ttcctgtata actttattct
     2101 cttcctctga aggtttgtgt tgaggtgaca tcatatcttt taatgcacca cttttatacg
     2161 tcgcatcagt tacgccaagt ttttctaata agccagaata atttaaacca gaaataatga
     2221 caccaattga tccagttgtt gtttcttccg tagcaaatat tttatctgaa ccagcagaaa
     2281 tgtagtaccc tccacttgcc gctgtatttt taaatgcagt atagatagga atatctagct
     2341 ttttaatttt tgccatttct ttagcaatct cagcactttc atatattcct cccccaggtg
     2401 aatttacttc taataacact cccttaaccg ctttgtcttc ttgaattttc tttagctgtg
     2461 ttaaaaaatt ctgatggtta tattgttccc tactaaaaag attgctttca cctgtatctg
     2521 caataactcc attgacggat agtttgacaa tctttttatt tgatgcgcct tcttctaacg
     2581 tttcttctgt gatttcgtta gatccgtaaa aaatcttatt tattcctgtt agggttgtat
     2641 ttgatgcttc ctctttatct tttcctggaa ttagcgcgac gataatggat agtaacaata
     2701 atccgcacgc tactcccaca gcaatccatc ttcttttatt catataaact cctcctatat
     2761 tcttagtaaa aataattatt ttttatttag aaacacatcg acaatttgtt gccaatatgt
     2821 tttttctcct acaattaaag acaactctcc agttaatcca taacgaaacg gaaacgattg
     2881 agttgttttt aacgttcctt tcactgcata aaaaacacct ttctcagtgg cagtagaatt
     2941 ttctgaaatt tcatctaaag ttccgtcgat cgtaatcgga gccccacctt tttgatccaa
     3001 tttaaaatga accttcatcc cttctttaat ataggtactt tcattcgcag gtaaaagtgc
     3061 tgtaaatttc atttttgatt tttcgtggat cggataaatt tctgcaagca ctgttccttt
     3121 ggctagttct gcttgtccct ttgtttcttc atttaaatga accgttccag atacaggagc
     3181 ttttagtatt ccttttgaaa gttgatcgtc caaacttttg ataactgact ggttcttctc
     3241 ttgtttttca tttagctctg taactgtttg ttttacagtt gttattgctt gttctagctc
     3301 ttgctttcct ttttccgttt gactaattct gtcattctca gaagtagttg gtttaactaa
     3361 tttagctttt tctgactgat actgttctac ttcttttttt agttgctcaa tttgttcatt
     3421 tatgcttgct gaaattgtca gtttcacttg atttttttga tcatcagaaa cattatttag
     3481 ttgttcttgc caattctcat agtttgccat catttctttt gaaaaatctt ttaatgattg
     3541 attattactc caagctacct gtacttgttg ccattcttgt aatttactat ctgaactctc
     3601 aatttgtttt tctagttgtc tttttgtctt ttcgtatact tctaattgct tttgatcatt
     3661 taattcactt tgcttcaaag cgtagcggag tgattctttt tctgacaaca tgctctttaa
     3721 ttgattactg tagccaaaac tgtcatccgt tgaaaacaaa ttttttcctt tacttatact
     3781 ttcaataaat agctgagcca tctttatttg ttgagtaata cgttggtttt cttgttctat
     3841 ctgagccttt tcattctcta ttaaagaaca atccaatcta accaacacat cgtccttatg
     3901 aacaaattga ttttctttta ggaagttttc cattatttta gcttctaatg gcacttgtaa
     3961 cttctctatt ttttcggggt tcaattgagc cgttgtccga ataacaacct cttttttagc
     4021 aaaggttaaa aacaatccaa taatacataa aaaaagaatt acagggtata acacccaacg
     4081 gtagaagcta tgatgttgtt tagtatagat actagaatgt tcgaaccaat tattattctg
     4141 cataaattgt ttccctctcc tagatttccc ataatttttg atagatacca tcggcaaaac
     4201 gaagttcatc gtgcgttcct ttttctacca aatgcccttc atggagaact aaaatttgat
     4261 cacatgcttt tgctattggt aaatgatgcg caataaaaat aatcgttttt tcttttaaat
     4321 tgaggagata attcataatt gcatgttcta acaaggtatc taatccacta gttgcttcat
     4381 ctaaaattaa tatgtccgga ttttttaata aagcccgtgc taaggctaat cgttgtcgtt
     4441 gccctcctga aagattattt ccaccttctt ccaacaatgt ctcgtaacgt aatggctgtt
     4501 gattaataaa ttcatttagt tggactatct cacaaacttc tattatttgt tcgaaacttg
     4561 gtgggctatc taaaccgaat ataagattct caatgattgt tccactaaag aaaaatgact
     4621 cttgaggcaa atatgtcact cgatcgcgta atatctgaaa aggaatgtct aagtgattaa
     4681 tgtttccata atggatcgat ccttctgatg cttcataaaa acgaacaaat aactttgcaa
     4741 gagttgattt tccagaacca ctaactccaa ctatagccgt tttactgtgc gaaggaattg
     4801 tacatgaaat ttcgttcaac gttggggcct tcatgctata cgagaatgaa acgtttttta
     4861 cagtgatatc ttgttgaaat aattgttcag aaagcatata ttcaaaagac tggttcgatt
     4921 tttctgaatc aatcgcgaaa atttcattta aacgattatt tgcaacttga gccgtttgca
     4981 ttttcacttg taaattaata atgttttgta aaggatctgt caaaaatact agtaaagcat
     5041 tgtatgtgat aagttgacct agactaattc tattatccat aacaaaataa gaacctagcc
     5101 ataaaattaa tgcactgcta attaattgaa tcaaatgttt aattccttgt tgaatattat
     5161 caagcgtaac cgtacgaaat gattttttca ttaatttaac aaattcatgg tctatacggt
     5221 tatatacttt ctcttcaccg ctatatgctt taattgtttc tattccttta agactttcta
     5281 tgatactaga atttacagct gcaccggctg ccatttcttc cctgttcgat ttttcatagc
     5341 ttttcacgaa agcgaatatt gcaactacat aaaaaggcaa agagcataga gtgattaaaa
     5401 acaacgtact attctgaata gctaacgtaa ccccaacaag caacaccata ccaatatcta
     5461 aaaatattga taacgtagca cttgctaaag catcaataat cttactggca tcaagaaaac
     5521 gagaaataat ttcccctgat tttctcgtag caaaaaaatt cattggtaaa gttaacacat
     5581 gtttgaaata ctgaagcata attgccatac tcattcgctg tcctaaaata ataagaagat
     5641 aactacgact atattcaaac aatacccgaa ataaatagac agcgatcaag ccaatggaaa
     5701 ttatatttaa agttgaacgt gcttgattag ggatgaaaaa atcaagaata ccttgaaaat
     5761 aatatgagct tcctatgcca aaaaacgtaa tgaaaatcga tgctaaaacg atgtggaata
     5821 ctaatccttt ttgtttccaa attataggca aaaaagaact taatccagct actttttctt
     5881 taataggaat ataagtttct ctaggcatag gtaataaaag aatacctgtc cattcttttt
     5941 gaaacgcttc aatcgttttt ctattttttc cattagcggg atctgcaatc catagtacgt
     6001 ttcccttcgt tttataaatt acaacgtagt gcatatagct gttatcaacc aatatatgtg
     6061 caattaatgg taagataaga tctctttctt tccagatctc actatctgct tgaatagcca
     6121 tacaatcaaa agacaatttt tcaaatgtct ttttcaatcc aaatgctgaa gtaccctcta
     6181 aatcagtccc agataggtcc cgtaatttat gaatcgggat atcagtttta aaatatttta
     6241 aaatcatcga aaggcaagca acaccacaat ctttttcatc ttgttgctgc acaaaaggaa
     6301 atgattttat cacttatcta acacaactcc ttctaaaaaa tttttataga aaaattctca
     6361 atatagcttt aatacagtga aaatattgtc tcataattcc acaaaaactt tgaaaacagt
     6421 tcttgaacac taactttttc attcctgaat caaatttatt ttggaaattt tactattcaa
     6481 gaataaactt gataagtttc aggaagattt ttactgttaa tcaaatacgt atgttacagt
     6541 agcaccgtaa aaaatattat ttaaagaagc gtgtttaaac atgcaaaatg taaaggaact
     6601 aagtattgtg gaaatgcaca aactgttggt ggaggcaata atgcttgggg aaaattgggg
     6661 caagttgtag gtggacttac cacaggggca gtaggtggtg caggtttagg aacagcaatt
     6721 tgcggaccag cttgcggtgt tgttggaggg ttatatggag ctgtcgctgg aggtgctgca
     6781 gctggctggg atgctcgaaa aaaataaaaa aggagaataa gtagtatgca tattaaaaac
     6841 acaaaaacaa catttatatt atcttcagaa gagttgaaaa atattcaagg tgggagtgcc
     6901 gtgggagttt taggcactac tttttctggg gcaacagcag gagtaaaact ttgttcagcc
     6961 ggaggacctt atgcaatagc tgcttgtggt gtaggtggag ctttattagg tgctggattc
     7021 agcatgtgga ccggtgcgta aacaattaaa aaatatcaaa cctcaagatt ggtttgtgtc
     7081 gattacgaac ataggtatgt tctttatctt ttcaaaagtt tggaatggac agaacttttt
     7141 actaaagctt cttgtgctag tttttataat tatattaaat ttttatcttt tattaaagtc
     7201 agataaaaat tctttggaaa tgtaatggta gagatatttt tacaaaagga cttgcagcgc
     7261 tgtagttggt gctgcaggag gacctatttc ggccgttaca ggaggtattg gtaatgtact
     7321 tgtcggatgt gcattaaagc ctgttcctgc aagttaaatt tataaaaaca ggaaagtaat
     7381 tatctcaaat cctatcaagt acaatcatag gattgcactt gacaggacaa attttttaac
     7441 taaaaaatga aaaacacttt ctaaaaatta gtagaatttg ccaaatcttt cgcatcttcc
     7501 accatacatt taattttaat tcccctttat ggtacttaat aaaaacaaga aaagaactat
     7561 tcatttggcc cttttttggt tccagaccat agaaatattc ctccagtaac taatattgct
     7621 gttatgcacg taactaataa cagctataaa aattggtaca actttaatag gattcagaaa
     7681 aatttgcact agtgaaggaa tctattttat tagaaaaatg caatctccct aaagaaaaaa
     7741 ccactaaaca gtgctttctt tcaacttctc cttaattttt tgtgctttcc ctaaagtaaa
     7801 gtttctggcc aactctgaat caaaatatag tgaacgtttt ttaaaatcta tttctttaac
     7861 gttaaagata tttactaaac acgctcgact aattcttgtt aacataggat atttcttttc
     7921 aagctcacta attcttccat aaaattcgta ctgcccatct ttagtacaca atgataatct
     7981 atgtggtaat tttgaagctt ctacaaaata aatgtcattt atatcaaaag taaatgtttg
     8041 agaacctatg gaaaaaacaa atgcttgatt cgtaacctgt tttgttgcat ctatcctttc
     8101 ttgcgctaat aacaataact caacaatctc gtttcgatac tcatctagtg tttgatcttt
     8161 tgttacaaat cctaacgttt caactcttct ttttattgtt acgggtaaca tctcatcatg
     8221 agtagttgta aaaatgattt ttgcttgaac atcatatttt ctgattactt ctgctaattc
     8281 tataccgtta acttcatgat ttaaatcaat atctaagaaa tatattcctt gctttggtcg
     8341 gaattgtttg aggtattttt taacttctaa cggactttgt gttttcaata ctatcttaaa
     8401 tactttatcg tgaaaaagaa tgaaattatc aataattcgt tctaactgat ttaattgtat
     8461 aatttgatct tcacataata ttattggata actcataaat cctccttccc tcaaaacaac
     8521 aaaataactt gagtagtaaa ttgatacgga tcgttttgat agttcacaaa catattggga
     8581 ttctttttat tgatttcctc tatactagat aaacctaacc cttgatgtcc cttttttgtt
     8641 gttattcctt ttttcattag agtatctata gagagtagta tctctctaca actattcgat
     8701 atcgaaattt ctatctgttt atcatctcgg taaatagcta gagacagttc tctatcttga
     8761 ctctctatag atgcttcaat tgcattatcg agaataattc caagcaccct aatacagtca
     8821 aatattggaa caggaacttc aataatagta cttggacatt cgaatttaca acaaatctta
     8881 ttgttatttg cttggtatag cttcgctatc aacaaacttt tcaaatattc attttttata
     8941 ttttttaaat aacgatattc aaacttattg gttagataag aattagaata gatctctaat
     9001 tcttttattt ctttttccaa tatcgaatca tggttttgat tagcgatttc ttttaaactc
     9061 agcaataaat ttttatagtc atgtctaaat ctggcaaatt tttcttgatc tttttccagt
     9121 gaatcagtat atttttttag ataaaccaat tcttttcttt tcaattgctg ttcatattgt
     9181 tctcgttgcc taatagttat tctaataaat aaaaatagaa caacaaacat ttgaatgatt
     9241 aaaaatatta agacaccaaa cacaaaatga tcaaatactt gatattgatg agcaacataa
     9301 gaaacaaata aaccaattgt aagtaaataa atcatgaata tagcagtaag tttagaactg
     9361 gactgttcaa caatcctatt tatagctaat tttctatata accaaataaa catcagcaaa
     9421 aacaaagaat caagcattat ctggaatact acataaccat acccttttat cccttcaaat
     9481 gaaaacatat acgtcataac catggaagta ataatagatc tgaaatcatc aataagcata
     9541 cataatagta atttatttaa aagcactata ttagttttct tcttttcctt taatataaaa
     9601 taacttccga ttataggtat agctgcggaa acatcagtaa ataattcaac aaaaaaagtg
     9661 agtggtatca ataaacatac aaaaagatat tttcttttgt taagatcagt attagtcata
     9721 taaaataaga gaaaaaagaa aaagtttccc acgaacattc ttaatagata agcttttgta
     9781 tcgatagtaa tcaagagatc ccctcctgat tttaggatat cataaaaaaa attttagtta
     9841 cactttttaa ataatgaaaa aacacattcc actaaatttg atgccggttt tccttgaggt
     9901 tttgttccag cacctccttt aattttttta agctcttgat ctgaacattg tttagcattt
     9961 agtctatttt tttcttccat tacgattccc cctacaaact ataaatactt ttatgtcact
    10021 gattcaatat acctattttt ctaatca
//
LOCUS       DANJLF010000085         9867 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000085 DANJLF010000000
VERSION     DANJLF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            205..684
                     /locus_tag="PWT25_002391"
     CDS             205..684
                     /locus_tag="PWT25_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022433.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="PWT25_002392"
     CDS             717..2945
                     /locus_tag="PWT25_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1022434.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="PWT25_002393"
     CDS             3074..4717
                     /locus_tag="PWT25_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL1022435.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="PWT25_002394"
     CDS             complement(4790..5335)
                     /locus_tag="PWT25_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL1022436.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="PWT25_002395"
     CDS             complement(5358..5843)
                     /locus_tag="PWT25_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1022437.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="PWT25_002396"
     CDS             complement(5833..6645)
                     /locus_tag="PWT25_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL1022438.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="PWT25_002397"
     CDS             complement(6784..8316)
                     /locus_tag="PWT25_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL1022439.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="PWT25_002398"
     CDS             complement(8425..9456)
                     /locus_tag="PWT25_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1022440.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3283 a         1832 c         1898 g         2854 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagattt
//
LOCUS       DANJLF010000086         9414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000086 DANJLF010000000
VERSION     DANJLF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            601..1836
                     /locus_tag="PWT25_002399"
     CDS             601..1836
                     /locus_tag="PWT25_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022441.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1920..3008
                     /locus_tag="PWT25_002400"
     CDS             1920..3008
                     /locus_tag="PWT25_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022442.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            3005..4426
                     /locus_tag="PWT25_002401"
     CDS             3005..4426
                     /locus_tag="PWT25_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL1022443.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            4462..5178
                     /locus_tag="PWT25_002402"
     CDS             4462..5178
                     /locus_tag="PWT25_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL1022444.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            5197..6780
                     /locus_tag="PWT25_002403"
     CDS             5197..6780
                     /locus_tag="PWT25_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HDL1022445.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            6915..7493
                     /locus_tag="PWT25_002404"
     CDS             6915..7493
                     /locus_tag="PWT25_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022446.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            7439..7957
                     /locus_tag="PWT25_002405"
     CDS             7439..7957
                     /locus_tag="PWT25_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022447.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            7965..9005
                     /locus_tag="PWT25_002406"
     CDS             7965..9005
                     /locus_tag="PWT25_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL1022448.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            9254..>9414
                     /locus_tag="PWT25_002407"
     CDS             9254..>9414
                     /locus_tag="PWT25_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1022449.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         3422 a         1040 c         1742 g         3210 t
ORIGIN      
        1 taatagaaat taacaagtta caaaaaaata ttataaagaa taatattttc taaaaagtaa
       61 actatagtaa tgtggattat caccttccaa cagtatataa aatgagcgta aaaacggcta
      121 tttttgcctt aacaacgatt tttttgtata caaatacgat atgctcatta attattggaa
      181 tttgtagtgc tgtttaaatt aatgaaggtg taattatatg agtgatcatt ttgaaaaagt
      241 ttgttgagaa gatgtgcttc tagtcatgtg atgggagaaa ataatttaac tgttagagtg
      301 aatagttaaa ttattctggt tatatgaaaa aagtagtttc taaaataatc ttgcagggat
      361 taacttaaag aaattgagag aatagtttct ttgaaatttc tatatacaat gagaagctga
      421 aatttacttt tatttgatga ctggaaatag aaattaaata tattgtattg ttacattaaa
      481 gaaaacttgt atggaaaaat cagtacaata ccattattta atgttcttca cttaaagcgc
      541 aaaaaatatc agtttattaa actcgtatat agaacaaata tatgtagacg gggcgaaacg
      601 atgagtgtaa aaaaaatgct atttatggta agtacaataa ttgtattaac atcccgattt
      661 tggggaatta atactagttt atctatgcga tattttatta tggcaatatg ggatttatat
      721 tttatgttaa gctatttttc ttatcgtgag aaaacatata aaaaatacgt tatcaagaat
      781 aacttttggt tgactataat gccgtttgtg atattttcta tctatactct aataatatgg
      841 ggaattggtt caaatgcaga atttgaaaat tttataaagt tatgttccac gttgctttat
      901 ttaattcttg catatggtta cgcatgtaca gcattttact tatttaaggc agaaggaatt
      961 gattttattt tttggtcagg tgttatttca tatatgagtg gttcagtttt ttatttaatt
     1021 gtatcttatg gtttaaatgg tttaatttct tatataaaaa gtcttataac aggtgaaaca
     1081 aatactgcaa attttgctat ggaggtacat gatttaactt ttgcgatggg ggtttttttc
     1141 ctttattacg tattttttga aaataaaaat acaaaaaaac ataagataaa aattatcgta
     1201 tctttaattt taattttcat gggattgaag agaattgaac tgttagcttt aagtggtgca
     1261 attctagtgt attatgtttt attaaagtgg ggaaaaaaga ttcaaatccg tgcattagtg
     1321 tgtactattt gtgcaactgt tatttcgtta gtgtttattt acattataga tacaggaatt
     1381 ttggcattgc ttatgtcaaa tttgggcatt acagatgacg gtgcgaggct ttcattttat
     1441 ctatattcat ctaaatttta tgaacttggt ttaggatatg taggaacagg atggacatgg
     1501 ttttcaaaat acttttttaa tctttattta tcgcacttta ggttaaatgg tggaagaatt
     1561 gcagcaagcc tacacagtga tatattaact atttatatag aagttggatg tatagttttt
     1621 attatatgga tgttttggtt attttttgtg aaaccgataa gactaactaa aaaatttgga
     1681 ttgaaaacag gggagtgtgt attattatta actatatata tgtttatatt atatttgacg
     1741 gataatacgc tcacttatcc agacactcaa atgatttttc tcttagtacc tatgatagtt
     1801 tcgtgggttg ataatcaaca acgaatatcg gaatagatag ctaaagtata ttttaattat
     1861 gaatttataa ttagttgtaa aagttaatat ttaacggaca ggaagtaggg tgaaaaaatt
     1921 tgaaaaaaat tttagtgtta gcaaatacat attatcaaat aatagtagca atacaaatga
     1981 aaaatacaat tttttccgat gagtatgttg aaattattat ttctgatcat tctaaaaaca
     2041 ctaaagaaat tgcgaaaaaa atagaagaaa aaaaagtgtt tgatggctgt cattatttag
     2101 aaagcaaatc ttttgatgat aatattgggt attttcaaaa gattaaggac tgtcttcaga
     2161 tttcttgtgg taaatcaaat cgatatgcct attatttgaa aaactgtaaa agtgaatatt
     2221 ttgatgaatt tatttgtttt tcaatgggag aggtttggtt ggaaggaata tatgcttgct
     2281 tatatcgaaa taataaaaat gtacaggtat caattttaga agaaggtatt ttaagctatg
     2341 gagttgacat tattccaaca gcaagaacaa aagctatcgc agtactcaat tacttaaaaa
     2401 aaaggactcc actatataac aaaagctata atttttattg tttttttcct aatatatata
     2461 caggaagaga gaatacaata caaattccat tagttacagc tgggtcaaga accgcattgc
     2521 aactttcgga tatttttaac cctatgatag atatttataa agaaaaaaaa tatatatttt
     2581 ttacgtctgt ttatgatttt gagggcggag aacctgtcgg agaatatgaa ttagtatgca
     2641 aaatagcaga cttagtagga aaagataatc taattgttaa gacgcatcct agagatgtaa
     2701 gaacaattta taaagataat ggatttatcg aggataaaaa ttcatcaata ccttgggagg
     2761 cgattcaatt gtcaggcgat tttaacgata aaatatttat gacaattaat tcaggaagtg
     2821 ttatttcagg tagtacaatg tctgcaaaac ctgtacgcac gtattatatg tataaattgt
     2881 gtaacataga aggaaatccg tcttgcaaaa agaatgcgta tgatattgag cgattgctta
     2941 agcaaaaaac gatgaaaaac attttaaaga atgtgaaaat agcagaaagc ttagatgata
     3001 tactatgagc aaaactaaat atttaattaa aaatatggga atattaacaa taagtaattt
     3061 ttcttcaaaa attttagttt ttttgttagt ccctttatat acaagtgtat tatccacaac
     3121 agaatatggc acatatgatt tgacaatttc aacagccaca ttattgtttc caattttaac
     3181 cttaaacatt gtagatgctg tgatgcgctt tttaatggat aaagataaaa ataaaaaaaa
     3241 tatagcatct atagcgatta aagttatatt tttaagcgtg atagtctttg gatccgtaat
     3301 gtatttgtta aatattacaa aattatggca agatgtttat ggattagaaa aatatatttt
     3361 tttgtattat atatcctatg tatggaatca atttctaata caatttgcta aagggttaga
     3421 aaaagttaaa gctatgggca tagcaggtat tatcagcaca attgtaacaa taattgccaa
     3481 tattttcttc ttgcttattc tgaaatgggg attaattggt tttttcattg caaatatttt
     3541 atcacagatt atttcagcta cttatctgtc agtattagtg agaatatggg aatatatcga
     3601 ttttgaggct aaagatcctc agctaaagag agaaatgcta aattactcga ttccattaat
     3661 tgcaactgtt gttggatggt ggattaatag tacagctgat aggtatgtgg tttcctttat
     3721 agtaggtgtc tcggcgaatg gcttattgtc tgtggcatat aaaatacctc aaataattaa
     3781 tactttgcag ggaatattta tacaggcctg gcaaatttct gcaattaaag aatatggaga
     3841 taatgagact gcaaaatttt atggaaatac atttgttatg attaatttgt taatgtgcat
     3901 aacctgttca agtcttatca tattaactag accgttttca catattcttt atgcgaaaga
     3961 cttttatgaa gcgtggaaat atgttccatt tttattaata gctaatgttt taaattgtgc
     4021 atcaggtctg ttaggaccta tcttatcagc aaaaaaagat tctaaatcca tgatgtggtc
     4081 tgcaatcata ggggcattag ttaatgtgat tatgagtgta gtccttgttt ataagatagg
     4141 ggttcaaggt gccacaatag ccactgttat atgttcatat gtaatatatg ttgttcgtaa
     4201 acgagctgtg ggaaaagata tttttataaa gcattatcat attgttgtgg aatcttgggg
     4261 attattgttt gttcaggcaa tcatagaaat atattttaaa aattatgtaa tagaaattat
     4321 aattatgatt attttaatta ttataaatat cagttatatt aagcagttgt gttatagagg
     4381 aagaaacatt atttcaatga ttaagagtcg agagcacaaa cggtagacga tgttattttt
     4441 ataaaaagat ataatttatt aatggtcgat aattatgaaa ggaaaaatat tcttaaaaaa
     4501 aagagtatag gatgtcgtat gaaaatagtc gctatagtac caatgaagtt aaataacaaa
     4561 agattgccac aaaaaaacac gaaagctttt accaatggaa aaccattgtg ttattatata
     4621 ctttcaacac ttttaaaagt aaaacagatt gataaagtgt atgtctattg tagtaaccca
     4681 gatattaaag aatttatacc agaaggtgta acatttttaa agagatctag ttccttagat
     4741 caggatacaa cgagtattac acaggtatta gccggtttca caaaagaagt agaggctgat
     4801 gtatatgtaa tgacacacac aacagcacca tttattagcg aaaaatctat tgaaaaagga
     4861 ctagatgccg tgctgacagg agaatatgat tctgcatttg ctgcaaaaaa attgcaagat
     4921 ttcttatgga aagacggtga accatttaat tatgaattgg acaatattcc tagaacacag
     4981 gatttgccgc ctttatacga agaaactagt ggattttata tttataatcg caatgtgatg
     5041 accgagttgc atagaagaat tgggaaaaaa ccttgcatag ttgaagtagg tgagattgag
     5101 tcagttgata ttgatgaagc cgaggatttt atgattgctg atgcgattta taatcatctt
     5161 attttacaag gtagttaaga cagggggaag gttaggatga acaatgtaca gattttagat
     5221 tgcacactta gagatggggg ctattgtaac aattgggaat ttggatttga taatacaaaa
     5281 aaaattattt ctggacttat agaatcagga attgagatta tagaatgtgg gtttctcaca
     5341 aataaaacag tatacaataa aaatgttaca aaatatacga ccttagaaga attagcacag
     5401 ataattcctg aaaaaagaga aggaaaatta tttgtagcaa tgatgaacta tggcgaatat
     5461 aatatagagg atcttccaga ttatgatggt tcatctattg atggaattag gattgcgttt
     5521 cataaaaaaa attgcaatga agcgttgaaa ttatgtcgtg agataaagaa aaaaggctac
     5581 ttagtttttg ttcaagctat ggtatctctc aattatacag atgaagagtt tttagcagta
     5641 ataaaaaatg tgaatcagtt taaaccatac gctttttata ttgttgatag ttttggaatg
     5701 atgaaaggaa aagatttaac tcgtcttttt tacatgattg agcataatct agaaaaagat
     5761 atacagatag gttttcactc acataataac atgcagcttg catattctaa tgcacaaaaa
     5821 cttataaccg tacaaacaaa tcgaagcctg attattgatt cttcggtgta cggaatggga
     5881 agaggagcag gtaatcttaa cacagaacta tttgtagaat atttgaatga taatgcagga
     5941 aaaaaatatc aattaaaacc attattatat ataattgatg aaatactaaa tagtttctat
     6001 cagaagagtt attggggata ttcattacca aactatattt cagcagttca taatgctcat
     6061 cctaattatg caagctatct gtcatataag aatactttaa caattgaagc aatgaatgaa
     6121 atattcgaaa tgatggatga agaaaaaaag gtatcttatg ataaacaata tatagaagat
     6181 ttatactaca aatatatgga aactgggaaa atacaggagg aatatatgga agaactgaga
     6241 aaatttctct cagacaaaaa aattctactg attgcaccag gaaaaagttc tgtagatgag
     6301 gtagaaagaa taaaagagtt tgttaccaaa gaagaagtcg taataattag tgtaaatttt
     6361 gaatattctc atatagatat agattacgta tttttaagta atttaagacg ttttcatgaa
     6421 ttaccgaaag aaagcagaac taaatgtatt gttacatcaa atatacaagc atcggatgta
     6481 tatttacgga cgagttataa aaagttgtta tctgaaaggg aagttgtaag agacaatgca
     6541 ggattaatgg caattagatt tttagccgat atgaatgtgg atgaaatatt cttagctgga
     6601 tttgatggat attcacataa tgaagatgag aattatggtg agcaatccat ggaaattata
     6661 acaaaatttg cattattaga tgcaatgaac gtgcaaatga gagatgaatt gtcagaatta
     6721 gctaaaaaag taaaggtgac atttttaacg gatccacgtt gggttcgaat tgataagtag
     6781 tagtaataat gtttttaaaa ttttgataaa gagatagtga gagaataaaa atgggtaaag
     6841 tcttaaataa aaaagaagaa aaatatttaa gggacgatgg ggcgagatga aactctaatt
     6901 ttgaactttt aagaatcatt tctatattgt gtattgttgc ttaccatgct aatttgcgtt
     6961 taaattataa tgcagacgga attatagtac atggttcatt aactgctaac cagattttct
     7021 tgaattatat tggtatttgg ggtcagctag gagttaattg ttttatattt atttcggcat
     7081 attttttaag tgagaaaaga aacattcact ttgaaaatgt tttgggaatt gtattagaat
     7141 gtatttttta tagtgtatta gtttatggaa ttctttgtgc atgtggaata gaaacatttt
     7201 cattgaacag ctttgtaaaa gaaatattat catttgctct aggtagatat tggtttatta
     7261 catcctattt agcattgtat attctaagtc cgtatttaaa tatcatgctt aacaatctgg
     7321 aaaaacttca gttgcaaaaa ataagtatgt tattgtttat aggtgtatgc tgttatcgta
     7381 tgttatttaa aacatctttg tttggggatt gtgctgtttt aattgcacta tatgtcatat
     7441 gttatacact gaaaaaaacg ataataatat ttttgagaga cacagaatca taggaacaat
     7501 tgtatgtata acagttatac cacttctcat atccatagga atacttttca aatttatacc
     7561 aaatgcaatg gttaaccaga tggttcatat tggttctcca ttgatggtta tagcaagtat
     7621 atttttattt tatgtattta aaaatttttc attttacagt aagtatataa ataaacttgc
     7681 gagaaccagt ttaggggtat atattttaca tgaaaatcat tatttcattg atggtatgaa
     7741 gtctagaggc ttaatatggg atgggatatt tggattaggc aaaaggtttt tagataggga
     7801 tttttggatt tggtttataa ttgcagttgt agttgtgtgc ataatttgca tggctatcga
     7861 tgttgtaaga gaaaaatttg aaagacagat ttttaaaatt aagagtgtaa agaacctgtg
     7921 taaacttatg aatcatgtta taaatatata cccataatta ataaatgagg agaaaagaag
     7981 tgaaagttat atcattcgga gaattattat tgagacttgc agcacccggt tattcaaagt
     8041 tatttcaaag agatagtttg gagactacat tctgcggagg ggaagctaat gtagcggtat
     8101 ccttagcaat atttggatat caatcagaat ttataacaaa gcttccggat tcagatgttg
     8161 gatatgcggc agctaatgct ttgcgttatt ttggagtaaa tgttgataat attatatttg
     8221 gagagggacg catgggactg tattatttag aaaaaggagc agctcagaga ccatctaaag
     8281 taatttatga cagaaaattt tctgcaattt cgttagctga tagtgatgaa tttaagtgga
     8341 aggagatcct aaacgatgca aattggtttc attggacagg gataaatcca gcattgtctg
     8401 agaatctctt tgagacgtcc ttagatgcat gtcaggctgc caaaaaaaga ggtataacaa
     8461 tttcttgtga tttaaattat cgatccaagc tttggacatc agaggaagca aaagaaaaga
     8521 tggaacagat actaccattg gttgatattt gtatttgtaa tgaagaagat gcagaaaaag
     8581 tgctaggaat taaagctgag catacaaata ttaatagtgg agaattaaat aaagatggct
     8641 atgtgcaggt ggctagggag attcacaaga agtttggatg taggtatgta gctacaactc
     8701 tgcgtagaag ttattcggca agtagaaatg gttggagtgc aatgttatac gaagcagaaa
     8761 aagataaagc gtatttttcc acagaataca acatacaaat tgttgatcgc gttggtggtg
     8821 gagacagctt ttctgctggt ataatatatg cgttatgtga aaatatgtcc ggacaaaagg
     8881 ctattgaatt tgcgactgca tctagttgtt taaaacatac tatagaaggt gattttaatc
     8941 gaactacaat tgaggatgta tataatttaa tgaatagcgg tggaaatggt cgcgtgttgc
     9001 gttagtcaac atataaagat aaatcacagt aatttttatg gtgattatct gtcttagtag
     9061 tgattagaat aacgtgaaaa taaattgttg agcgtttata ctgttttgaa aaataaattt
     9121 aacatattga aaaatacaaa tatatacgta tattataaag cgccgattgt aaaattaagc
     9181 tagacaacta aaacagtcat ttgtgctaca ctcaaaatag ttccgaccaa agaattataa
     9241 ggagtgagtg caaataacct atacctatct tacaacggat gaacttgtaa taatagagtc
     9301 ttatttgaaa attaaccaat ctgttgctaa aactgcgcat tgcttgaatc gttcaagtca
     9361 aacgatccat aaagtatatc tgttcttcaa gcaaagaaaa tcagccttag aata
//
LOCUS       DANJLF010000087         9361 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000087 DANJLF010000000
VERSION     DANJLF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(92..760)
                     /locus_tag="PWT25_002408"
     CDS             complement(92..760)
                     /locus_tag="PWT25_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1022450.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="PWT25_002409"
     CDS             894..1181
                     /locus_tag="PWT25_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022451.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1597..3135
                     /locus_tag="PWT25_002410"
     CDS             1597..3135
                     /locus_tag="PWT25_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL1022452.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3132..4097
                     /locus_tag="PWT25_002411"
     CDS             3132..4097
                     /locus_tag="PWT25_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022453.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4084..5406
                     /locus_tag="PWT25_002412"
     CDS             4084..5406
                     /locus_tag="PWT25_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1022454.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5469..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5647..6390
                     /locus_tag="PWT25_002413"
     CDS             5647..6390
                     /locus_tag="PWT25_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1022455.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6405..7397
                     /locus_tag="PWT25_002414"
     CDS             6405..7397
                     /locus_tag="PWT25_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1022456.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7409..8146
                     /locus_tag="PWT25_002415"
     CDS             7409..8146
                     /locus_tag="PWT25_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022457.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8136..8858
                     /locus_tag="PWT25_002416"
     CDS             8136..8858
                     /locus_tag="PWT25_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL1022458.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8942..>9361)
                     /locus_tag="PWT25_002417"
     CDS             complement(8942..>9361)
                     /locus_tag="PWT25_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL1022459.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1441 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1501 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1561 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1621 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1681 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1741 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1801 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1861 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1921 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1981 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2041 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2101 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2161 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2221 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2281 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2341 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2401 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2461 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2521 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2581 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2641 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2701 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2761 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2821 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2881 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2941 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3001 aaattaagcg gaaaaataac ggaggatcaa atgattgata tgaattacca agtcaatgtc
     3061 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3121 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3181 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3241 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3301 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3361 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3421 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3481 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3541 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3601 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3661 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3721 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3781 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3841 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3901 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3961 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4021 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4081 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4141 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4201 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4261 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4321 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4381 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4441 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4501 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4561 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4621 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4681 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4741 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4801 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4861 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4921 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4981 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5041 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5101 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5161 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5221 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5281 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5341 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5401 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5461 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5521 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5581 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5641 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5701 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5761 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5821 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5881 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5941 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6001 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6061 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6121 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6181 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6241 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6301 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6361 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6421 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6481 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6541 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6601 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6661 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6721 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6781 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6841 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6901 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6961 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7021 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7081 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7141 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7201 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7261 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7321 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7381 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7441 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7501 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7561 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7621 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7681 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7741 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7801 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7861 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7921 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7981 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8041 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8101 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8161 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8221 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8281 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8341 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8401 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8461 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8521 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8581 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8641 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8701 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8761 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8821 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8881 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8941 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9001 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9061 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9121 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9181 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9241 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9301 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9361 a
//
LOCUS       DANJLF010000088         9357 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000088 DANJLF010000000
VERSION     DANJLF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PWT25_002418"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PWT25_002418"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL1022460.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PWT25_002419"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PWT25_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL1022461.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PWT25_002420"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PWT25_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL1022462.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PWT25_002421"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PWT25_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL1022463.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PWT25_002422"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PWT25_002422"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL1022464.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PWT25_002423"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PWT25_002423"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL1022465.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="PWT25_002424"
     CDS             complement(8053..8523)
                     /locus_tag="PWT25_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL1022466.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="PWT25_002425"
     CDS             complement(8569..8994)
                     /locus_tag="PWT25_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL1022467.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="PWT25_002426"
     CDS             complement(9061..9330)
                     /locus_tag="PWT25_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL1022468.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3286 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttt
//
LOCUS       DANJLF010000089         9281 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000089 DANJLF010000000
VERSION     DANJLF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..435
                     /locus_tag="PWT25_002427"
     CDS             <1..435
                     /locus_tag="PWT25_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL1022469.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="PWT25_002428"
     tRNA            complement(759..842)
                     /locus_tag="PWT25_002428"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="PWT25_002429"
     CDS             1370..1870
                     /locus_tag="PWT25_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1022470.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="PWT25_002430"
     CDS             complement(2060..2404)
                     /locus_tag="PWT25_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL1022471.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="PWT25_002431"
     CDS             complement(2558..3478)
                     /locus_tag="PWT25_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1022472.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="PWT25_002432"
     CDS             3665..4156
                     /locus_tag="PWT25_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL1022473.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="PWT25_002433"
     CDS             complement(4180..6555)
                     /locus_tag="PWT25_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL1022474.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="PWT25_002434"
     CDS             6881..7507
                     /locus_tag="PWT25_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1022475.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="PWT25_002435"
     CDS             7530..8234
                     /locus_tag="PWT25_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1022476.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="PWT25_002436"
     CDS             8426..9259
                     /locus_tag="PWT25_002436"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL1022477.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DANJLF010000090         8961 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000090 DANJLF010000000
VERSION     DANJLF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            173..1387
                     /locus_tag="PWT25_002437"
                     /pseudo
     CDS             173..1387
                     /locus_tag="PWT25_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1566..1895)
                     /locus_tag="PWT25_002438"
     CDS             complement(1566..1895)
                     /locus_tag="PWT25_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1022478.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1885..2157)
                     /locus_tag="PWT25_002439"
     CDS             complement(1885..2157)
                     /locus_tag="PWT25_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL1022479.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2608..3411)
                     /locus_tag="PWT25_002440"
     CDS             complement(2608..3411)
                     /locus_tag="PWT25_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1022480.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3389..4513)
                     /locus_tag="PWT25_002441"
     CDS             complement(3389..4513)
                     /locus_tag="PWT25_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022481.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4529..5344)
                     /locus_tag="PWT25_002442"
     CDS             complement(4529..5344)
                     /locus_tag="PWT25_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1022482.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5334..6206)
                     /locus_tag="PWT25_002443"
     CDS             complement(5334..6206)
                     /locus_tag="PWT25_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1022483.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6291..7580)
                     /locus_tag="PWT25_002444"
     CDS             complement(6291..7580)
                     /locus_tag="PWT25_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1022484.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7808..8452)
                     /locus_tag="PWT25_002445"
                     /pseudo
     CDS             complement(7808..8452)
                     /locus_tag="PWT25_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2783 a         1900 c         1429 g         2849 t
ORIGIN      
        1 ttggtcgact aaattttacc aaaagtgagt ctctttttga gttgaaacga agaaaaagtg
       61 ttagtagaaa atcgccttcg attttccacc aacacaaaat attatagaag caaaatcaat
      121 tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag ccaagaaaaa
      181 gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc
      241 tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga aggaaacgat
      301 tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa agcaacgcta
      361 tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc gactcagcca
      421 ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa aagtatcttt
      481 gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta cattgaaaga
      541 gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat tgatggttga
      601 tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggtga
      661 aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa caagctattt
      721 cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga acgccgctta
      781 cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat
      841 tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga atgagcttcg
      901 taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttctgctaaa
      961 aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc gagcaccaaa
     1021 atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt ctgcgcctct
     1081 aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca aagaccctga
     1141 cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggagaa
     1201 gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct atccttattc
     1261 caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag tatcctacgg
     1321 atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat taatcaatgt
     1381 aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt gattttgcac
     1441 aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag gttcattctg
     1501 acccagattt gattttgtac aacccatcag tccttattga caaagagccc ttctttttag
     1561 tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg acttaattcc
     1621 ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag agttcctacg
     1681 tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc aacgtatttc
     1741 agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa cgaaattgtg
     1801 tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc agctgtgaaa
     1861 gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt cttcaatttt
     1921 ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa tattatggat
     1981 tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct caacaatttg
     2041 cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa taatgctttc
     2101 tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt tctccacctc
     2161 tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac aactactttt
     2221 gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag tgctaaatgg
     2281 ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg aacactcaac
     2341 gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg tgtctgaaaa
     2401 ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg aagaccgata
     2461 tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg tttattgata
     2521 gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta aaaagtgaat
     2581 gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt ttttcatgcg
     2641 ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt ttccgtcaat
     2701 taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg ggtattcatc
     2761 tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt cagcactgct
     2821 accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa tcaattcagc
     2881 aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg gatgatgaac
     2941 caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt gaatcatctg
     3001 tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg aaataattcg
     3061 taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt caacactcat
     3121 tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt aaggtgtggt
     3181 tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc ccccttcatg
     3241 tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt cttgtaacaa
     3301 tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag taaacaagtt
     3361 agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca tggctattcc
     3421 ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga taatcaaaaa
     3481 aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc caagtaggtt
     3541 tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca taactttgac
     3601 ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt gaaaaggtta
     3661 cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt tgtttttgcc
     3721 aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga tcataagtaa
     3781 ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta tttttaggtt
     3841 tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc aataaaacaa
     3901 tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca aaagtttgtt
     3961 tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa cgcacatcca
     4021 aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc gcacgagcca
     4081 atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct tggtatggcg
     4141 ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct gtcttaattt
     4201 tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga tataaagcat
     4261 aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt gctagttgtg
     4321 tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt ctcagcaaag
     4381 tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg gccacagcta
     4441 aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt cttaattcaa
     4501 gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca aaagtcccga
     4561 taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta attaaactaa
     4621 tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc tgcatcgcca
     4681 acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca tactcattcc
     4741 aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta agaggaaaaa
     4801 caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca tctagaaggc
     4861 tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca tcagcaaatt
     4921 gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc aacaaataat
     4981 taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata aagtaccaga
     5041 ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa gctacgccta
     5101 acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt ttgataattg
     5161 gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt atcaaactag
     5221 taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata aacaataatg
     5281 gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag cgtttaattt
     5341 tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac agcaaatatt
     5401 aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc aaaagccttt
     5461 tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg agtgatgatc
     5521 ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa taaatatagt
     5581 cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat gggtacacga
     5641 tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc aaaaatgaca
     5701 atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc aattacccaa
     5761 ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt
     5821 ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac caaagaaatc
     5881 acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat aactattcct
     5941 aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag aacggtatag
     6001 gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt tacatagttg
     6061 gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa actcaaatag
     6121 atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc tggcgccaga
     6181 tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat gtcaaaagca
     6241 gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt
     6301 aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt acctgataaa
     6361 attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc tccaggaaat
     6421 gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat ctgattttca
     6481 gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg tggtaaataa
     6541 ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt taagaaatcc
     6601 caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc gccaccagca
     6661 ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc agtaaaagta
     6721 ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc gacgttacca
     6781 tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg gtcttttacc
     6841 atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt agctttgcca
     6901 tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca attatcagca
     6961 ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt agaaaaagtt
     7021 tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt taatatatct
     7081 tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg tttaccatct
     7141 ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc agcattatcc
     7201 ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc tgcaaaataa
     7261 tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc aacagaagct
     7321 tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac aaccttcact
     7381 ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc acctagggta
     7441 tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt tgttgtcttg
     7501 gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac aacccctata
     7561 acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg ccatttttct
     7621 aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg taaataattc
     7681 gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct aagaataaag
     7741 cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt tgattataaa
     7801 atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt gaggttatat
     7861 tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact gtccatacag
     7921 aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac catccttcat
     7981 gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg atcaaatctg
     8041 cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta taaaatatat
     8101 gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca atttgtggcc
     8161 agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct tcttttaaat
     8221 cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc tgctcaaggg
     8281 ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta ccatctgttt
     8341 gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga agaaatgccc
     8401 agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc attttgaccc
     8461 ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc gttaatttct
     8521 tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc
     8581 tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc accaattaag
     8641 tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag ctattccttc
     8701 ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg ttcaatggct
     8761 tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac caagtctttg
     8821 atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc tagcgattgg
     8881 tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct agcccacaaa
     8941 aaaagctgtc ctcacagaca g
//
LOCUS       DANJLF010000091         8921 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000091 DANJLF010000000
VERSION     DANJLF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="PWT25_002446"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="PWT25_002446"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL1022485.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1713..3059)
                     /gene="gor"
                     /locus_tag="PWT25_002447"
     CDS             complement(1713..3059)
                     /gene="gor"
                     /locus_tag="PWT25_002447"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL1022486.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3193..4446)
                     /locus_tag="PWT25_002448"
     CDS             complement(3193..4446)
                     /locus_tag="PWT25_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL1022487.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4452..5375)
                     /locus_tag="PWT25_002449"
     CDS             complement(4452..5375)
                     /locus_tag="PWT25_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL1022488.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5596..8088)
                     /locus_tag="PWT25_002450"
     CDS             complement(5596..8088)
                     /locus_tag="PWT25_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL1022489.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8095..8562)
                     /locus_tag="PWT25_002451"
     CDS             complement(8095..8562)
                     /locus_tag="PWT25_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL1022490.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8698..8771)
                     /locus_tag="PWT25_002452"
     tRNA            complement(8698..8771)
                     /locus_tag="PWT25_002452"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8735..8737),aa:Arg,seq:acg)
     gene            complement(8829..8902)
                     /locus_tag="PWT25_002453"
     tRNA            complement(8829..8902)
                     /locus_tag="PWT25_002453"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8866..8868),aa:Pro,seq:tgg)
BASE COUNT         2479 a         1884 c         1485 g         3073 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
     1081 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
     1141 caccgtttat cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg
     1201 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
     1261 cactatttgt tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt
     1321 cacttgattt gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat
     1381 ggtattcttt taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt
     1441 ttaaacatct gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga
     1501 acttaagagt gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa
     1561 atctccaact tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa
     1621 ctaaaaaaca aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc
     1681 aataaaaagc tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct
     1741 cctgttggat ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata
     1801 gccacggcaa aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg
     1861 attctttctt cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt
     1921 aatgcaaagt acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat
     1981 ttttccaatg cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg
     2041 actgtcggaa caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct
     2101 gataaacgtc taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca
     2161 ccaacagcaa aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct
     2221 tttttgtcca attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt
     2281 ccaccggcaa aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta
     2341 tattcgtcat tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca
     2401 gcttcttggt acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt
     2461 cgttcttttc gatatgccca catgacttca ctacctaaac cacgaaatac acctgaaagt
     2521 tcagccgcaa tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca
     2581 aaaaatccat ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg
     2641 cctccagtag cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt
     2701 tgactatcga taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct
     2761 ctttgataag caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg
     2821 aaatcaacat tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt
     2881 tccaaaatcg tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat
     2941 gttccgccca attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg
     3001 tttgctgaag caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt
     3061 ttgttcctcc ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag
     3121 aaaacaagtg taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata
     3181 tcagcttttt ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac
     3241 tggcatagtt aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt
     3301 atgcataact tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc
     3361 atctcctaca gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata
     3421 agataatact tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg
     3481 ttcgttttca gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt
     3541 ccataactcg tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact
     3601 gtctacagcc gctttataga catttgacac cgtagataca taatggatcg atttcattct
     3661 tccttcgatc ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat
     3721 tgacatatcc actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact
     3781 gtttcgttct tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga
     3841 acatccgcca cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga
     3901 ataagagata cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg
     3961 aatctctgcc acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc
     4021 ttctttccag aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat
     4081 cggcagacct ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac
     4141 tgcagaaata ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc
     4201 ttcatgggtg accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc
     4261 aactccttca gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc
     4321 attcccgcca atatatactg catctgctcc ataatggatc gcggttttta acttttccag
     4381 tgttcctgct ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt
     4441 catcatactc cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca
     4501 cggttttcag gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc
     4561 cagctacctt ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat
     4621 ttttcgcctg gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt
     4681 tcattcaatt cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta
     4741 tcttcataaa tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt
     4801 tcacgatctt tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt
     4861 ttggattgat ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt
     4921 tcttccatcg ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg
     4981 ccttttttcg cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa
     5041 atatacggta tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca
     5101 actgtgatca catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc
     5161 attttttcag gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt
     5221 gctaattcta ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca
     5281 aattgctctt caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt
     5341 tcaatagttt ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata
     5401 atagtagcat agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa
     5461 aattatttca caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga
     5521 ttgtgacaaa aatcttgtca caacctctta atcaagtctt actattataa aaattcagct
     5581 acacaggcca agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa
     5641 tgtgatctta ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc
     5701 aagcaaagcc tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg
     5761 tgcaccatat tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat
     5821 tttcacttga atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac
     5881 gattgtatgg atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt
     5941 caagaattca ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt
     6001 gtgatcttta gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt
     6061 cgctccgata tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt
     6121 agaatctgtt aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg
     6181 tgctttctcc acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt
     6241 caactgtcca ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt
     6301 actgtatttt tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa
     6361 catgacttct gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa
     6421 catgaatgaa ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc
     6481 acgtgaaact gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc
     6541 cagttctaac aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca
     6601 ctccgataca actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg
     6661 ttttgcttca attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg
     6721 taattgagca gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag
     6781 ttcatcgatt tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg
     6841 gactttcgct gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt
     6901 gatataacga acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc
     6961 atggtgctct tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt
     7021 tggctcatct acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata
     7081 tttttgatat tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc
     7141 cggtttcaag atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt
     7201 atgcaattca tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt
     7261 tttcattcga tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc
     7321 aagcatcatc aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg
     7381 agctaaccct tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt
     7441 attctttgtc cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac
     7501 aactgggtcc agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag
     7561 cgtaggtgtt ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt
     7621 cattcctttt ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc
     7681 ttttgaaagg ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt
     7741 taataagctc atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc
     7801 gctgctagct aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt
     7861 agggtaagaa cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt
     7921 ttcattcaaa tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa
     7981 ggctagtaaa acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg
     8041 tgcaatttct aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt
     8101 cccatcttca taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc
     8161 ttcatattca ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc
     8221 tttttttgta attatttggt cttcataaag tttttgaata atagcaaaag catttttctc
     8281 actgattgta tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat
     8341 ccttactttt gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc
     8401 acgttggatg gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa
     8461 gaggtctgcc atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt
     8521 tatatatgat tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga
     8581 ctaatattga ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt
     8641 taaataaatc ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatcgacgc
     8701 gcccaagagg agtcgaaccc ctaacctttt gatccgtagt caaacactct atccagttga
     8761 gctatgggcg catattcaat ttaaaaaaat tataaatcat ttgaaaacaa aaagcaagat
     8821 aaaaaaaatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc
     8881 taccaagctg agctacttcc cgtttaaaaa ataaaaaaga c
//
LOCUS       DANJLF010000092         8640 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000092 DANJLF010000000
VERSION     DANJLF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..1007
                     /locus_tag="PWT25_002454"
     CDS             <1..1007
                     /locus_tag="PWT25_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1022491.1"
                     /translation="FKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYM
                     QQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVM
                     GTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFS
                     CVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKF
                     GETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIE
                     RSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW
                     "
     gene            1019..2023
                     /gene="neuB"
                     /locus_tag="PWT25_002455"
     CDS             1019..2023
                     /gene="neuB"
                     /locus_tag="PWT25_002455"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL1022492.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2020..3168
                     /gene="neuC"
                     /locus_tag="PWT25_002456"
     CDS             2020..3168
                     /gene="neuC"
                     /locus_tag="PWT25_002456"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL1022493.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3141..3818
                     /locus_tag="PWT25_002457"
     CDS             3141..3818
                     /locus_tag="PWT25_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL1022494.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3808..4950
                     /locus_tag="PWT25_002458"
     CDS             3808..4950
                     /locus_tag="PWT25_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL1022495.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4963..5940
                     /locus_tag="PWT25_002459"
     CDS             4963..5940
                     /locus_tag="PWT25_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1022496.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5940..6848
                     /locus_tag="PWT25_002460"
     CDS             5940..6848
                     /locus_tag="PWT25_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HDL1022497.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            6849..7601
                     /locus_tag="PWT25_002461"
     CDS             6849..7601
                     /locus_tag="PWT25_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL1022498.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            7606..8553
                     /locus_tag="PWT25_002462"
     CDS             7606..8553
                     /locus_tag="PWT25_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL1022499.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         3177 a         1097 c         1658 g         2708 t
ORIGIN      
        1 tgtttaaagg aaaaacaata ttagtaactg gcggaactgg ttccattgga agtgaaattg
       61 ttcgtcagct tttaaaatat gaaccaaaag ctatcagaat ttatagtaga ggtgaagaaa
      121 aacaatttta tatgcaacaa gagctctcaa atcacactaa tttgagattt ttaattggtg
      181 atatcagaga ctctcaaagg attgattatg catgtcgtga tgtggatatt atttttcatg
      241 ctgccgcaat gaaacacgtt ccagcaagtg aatataatcc tatggaagct gtaaaaacaa
      301 atgtgatggg aactcaaaat gtaatagatg cagcgatcaa aaataatgta aaacattttg
      361 taggtatcag tacagataaa gtagtgagtc ctactaacac catgggtgcg acaaagttat
      421 tgagtgaaaa attaataata gctgccaatc agtataaagg aaaaacagaa acaatatttt
      481 cttgtgtaag gtttggaaat gtcatggggt caagtggatc tgttattcct ctttttagta
      541 aacaaataaa aaacaataaa gatattactt tgacagatgt tgatatgaca cgatttatga
      601 tgtcaatacc ggatgctgca agattagttc tgtctgctgc tatgaattca aaatttggag
      661 aaacatatgt cttaaaaatg ccggcaatca atattttaac tttagctcaa tcattaataa
      721 aaatatataa tgatacacat gatgaaaaat atagaggtga aataaaagtt gtaggtatca
      781 ggccaggaga aaaattatat gaagagctaa tgactacaga cgaaattgaa cgctcatatg
      841 aaaatgataa aatgtatatc attccaccac actatcatgc tactgacgaa ctgtacaaag
      901 attatgaaaa aattaaaatc actgcatact cttcagatgc aacagaatta atgagtggac
      961 aggaatttat tagaatcgca aaagagaata atgtcgtgac ttggtaagga gttttatgat
     1021 gaaatcagaa gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa gtctagattt
     1081 agctttagaa agtattgata aagctaaaga atgtggagct aacgcggtta aatttcaaac
     1141 atttaaaacg aataagctag tttccaaaaa ggctccaatg gcaaaatatc agaaggataa
     1201 tcttaaagag gacagttctc aatttgaaat gctaaaaaaa ttagaattat ctcaatctga
     1261 ttttattaga ataaaagatt attgtataca aaataaaatt gattttttgt ctactccttt
     1321 tgatgaagaa agtgcagact ttctaaaaca aattggtgta caaggattta aaattggctc
     1381 aggagattta acaaatttac cttttcttag aaaaatagat atgtatgggt tgccagtttt
     1441 attatctaca ggtatggctg atattcatga ggtcagtgag gctgtagatt gtttcataga
     1501 tagtccagtt actattttac attgtacttc aaattatccg gctagttctg aagatattaa
     1561 tttattggcg attaacacat taaaagaaat gttcgggata cccgttgggt actccgatca
     1621 ttcattggga tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg taattgagaa
     1681 acattttacc ttggataagg aactacctgg tccagatcat aaagcttctt tagatcctca
     1741 ggaatttgca gattttgtaa agcatattag aaacactgaa atatttttag gtgatggtgt
     1801 taagcgagca atgccttctg aacttagtac aaaagaagta tcaagaaaaa gcctagtggt
     1861 cactgagaat ttaaatgtgg gagatattct tacagagaaa tctcttacaa taaaacgtcc
     1921 tggaaatgga atagctccta agtttttata tcaatatatt ggcaaaaaag ttaaaagacc
     1981 aataagtgca gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat acttatagta
     2041 agtggaacac gagctgatta tggaatatat aaacctgttg ctgagaattt actacaagat
     2101 aattgggatg tatcatttgt tgtttctgga atgcatttat cccataaata tggatttact
     2161 aaagaattga tcttagctga cggatttaga attagtggag aagttcaaag tttgttttta
     2221 gagaacaata agggaaacat ggctcggtct gtggcattag agataaatgg tttcacaaat
     2281 atatttgaac aaactagccc tgattttgta ttattactag gggatagagg agaaatgttg
     2341 gcagctgcga tagcttgttt gtatcttgga atcaaaacag cacatattca cggaggcgaa
     2401 gtgtctggta ctgtagatga atcaataaga catgccataa cgaaaatagc atctgttcat
     2461 tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga ttcttggaga
     2521 gtttttcccg tcggagctcc tagaatagat actattttaa ataatagatt acctagtttt
     2581 agagatataa aagaaaaata tcatctaaaa tttgaaaaga aaaaatacgt tttgcaagta
     2641 tttcatccag ttgtaaccga actagaaaat atagaaaaac aaattttagc tttgatcaat
     2701 tcaatggaac aacaatctca accaattatt tgcattttac ctaatagtga tgctggtagc
     2761 gaaattatca gaaaaatgta ccagaaatat ttaaaaacgg atattattta tattacgaat
     2821 ttagagccgg aggaatacct gactatatta aaagagtgca aatttatgat cggaaattct
     2881 tcttctggga taattgaagc tgcatcattc aaaataccag taataaattt aggaaccaga
     2941 caaaatggac gagaacaatc taaaaatatt ataaatgcaa atctaaatga aaatgaaatt
     3001 gaaactgctt tgaataaaat acaagatcct cagtttataa aaagcttgaa aaatgttaag
     3061 aacatctacg gtgacggcaa cagttctaga agaataacaa aaacattgaa tcaaatattg
     3121 aatgatgggg aagatattaa gtggattcaa aaacaaatag cttattaaaa tatactagtg
     3181 atccatacat tattattggg gctggcggac acgcaaaagt cattattgaa accttaagat
     3241 tagaaaataa gttaatatat ggattttttg atgataagca aaataaaatg caagatctga
     3301 aatatcttgg gaaaataaac gaattgaata gctgggcgga tagcaaaaaa aaaatctata
     3361 aatttattgt agctattggt gactgtgtag taagaaaaaa gattataaaa aaattgaata
     3421 tgccaagtga gcaatatgga atagctgttc atccaagtgc tatagtggca aaagaaattt
     3481 caattggtta tggaactgtt atttttgcga atgctgtaat taacccagat gctgtaattg
     3541 gagagcatgc tataataaac acaggtagca ttgttgagca tgattgtaga attaataact
     3601 atgttcattt atctccaggt gtatgcttag cgggtggagt acatgtaggt gtaggaactc
     3661 aaataggaat tggttctcaa tgtatccaga tgaagaaaat tggatcttgg tgtatgatag
     3721 gcgcagggag cactattgtt aaggatattc cctcacattc cttagcttat ggcaatccag
     3781 ctaaaattaa gaaagaagga attaattttg aattctagaa ttttattatc aagtcctcat
     3841 atgggaggaa cagaagaaaa atatgttgct aaggctttta aagataattg gatagcgcca
     3901 ttaggtgaaa atgtggataa atttgaagaa agtattacaa aatatactgg ttctaagggc
     3961 ggagcttctg cgttaagttc aggtacagct gccatacatc ttgccttaag actgcttaat
     4021 gttggaaaaa acgatattgt ctttgctcct tcatttactt ttatagctac agtaaatcct
     4081 attttatatc aaggggctgt accagtgttc attgactcag aacctaatac atggaatatg
     4141 agccctgttg cactagagag cgcattagaa gaagctgaaa gaaacaacca attacctaag
     4201 gcaatcatcg tagtgaattt atatggtcaa atggctgatt atgaacgaat tttagctatt
     4261 gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg ttctacttat
     4321 aagggaattt cgtcaggctt atttgggaca attgggatct attcatttaa cggtaataag
     4381 atcatcacaa cctcaggtgg aggagctcta gtgtctagtg ataaaaaata tgttgagcat
     4441 tcaagatttt tagcaacaca agcaagagat caagcaccgt attatcaaca tagcgaaata
     4501 ggttataatt accgtctaag caatatttct gccggtatcg gacgtggaca aatggaagta
     4561 ttgaatgata gagtagctaa acgaagagaa attttcaatc gatataagga agctttttct
     4621 ggaattgata gaattgaaat gatgccagaa gtggatggca tggtgccaaa tcgttggttg
     4681 tctactttaa cgatcaatga ttataattct attgatcaag tagttaacgt aataaataaa
     4741 ctaaatgaaa aaaacattga aacaagagct ttgtggaaac cactacattt gcaaccagta
     4801 tttcaagggg taaaaatgta ttaccatgat aataatctta gtgtgtcaga aaagttattt
     4861 aatagcggct tatgcttacc ttctggaagt aatatgacag atagtgaaca agaatttgtt
     4921 attgatgagt ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat aggaattatt
     4981 ggtttaggtt ccatggggaa aagaagactt agactcattc aagaacactt taatgatatt
     5041 ttactatgtg gtgttgatac taatgaggcg agacgaaagg aagttgaaaa ggaatttgga
     5101 ataccaactt ataatagtct aacagaggca aaaaatttgt ttgaaataaa ttcagttttt
     5161 gtttgtacat cccctataac ccatgaattt attataaaag aggctttaaa tttagattgt
     5221 aatatcttta cggaaatcaa tttattaaat gaatattatg atgaaataat cgaagtagct
     5281 aaaaagaaag atagattact ttatctatct tctacatttt tgaagagaaa agaaattcaa
     5341 tatattaagt ctaaagtgac gcataataaa aaaatttctt atcaatacca tgtaggacag
     5401 tatttacccg actggcatcc ttgggaagat tatcgcaatt tttttgtttc aaacaaaaaa
     5461 actaatgggt gtagagaaat attcgctatt gaattacctt ggattataga tacatttggg
     5521 aaagttaaat cgtttactgt aatgacccag aaattatcca atcttgatat tgattattcg
     5581 gacagtttca ctcttattat tgagcatgaa actggtatag ttggaacttt aagtgtagat
     5641 gttgtttcaa gagtagctaa aagaaattta actataattg gtgaaaagat tttaatagaa
     5701 tgggaaggta acccggattc tctattttca tggaatgaag aaacttctag tatgacagat
     5761 attcaattgt acgatgaggt tatgcatgat aataattatt cacatactat tattgaagat
     5821 gcatatttag aggagattaa agaatttttc acgcttttgg aaaagaaaat ggaaccccaa
     5881 tatacttttg aaaaagataa gtatattatt aacttaatta acgacattga ggtaaattaa
     5941 tgatgaaagt aatttttttt gggttagggt caattggaaa gagacatcta aaaaatctaa
     6001 taagctatgg aaaaaagaat tctattaatt ttgatataac agcttatagt agcagtggaa
     6061 aaaaagagtt tgaagaaata acgtatattc aaaatagaga cgatttggat acacattatg
     6121 atatagcttt tattacaaat ccgactttta agcatctgga aacactaaat gtaattgttg
     6181 aaaaagttga ttacatattt ttagaaaaac ctgctttttc tgagagtatt gatcttactc
     6241 aatataatta tgattacaaa aatatttatg ttgctgctcc actgaggtat aaaaatatca
     6301 tggaagttgt tcgtaatagt attcaaagta aaaaagtata ttctgtaaga gctatttgtt
     6361 ctacttatct tcctgattgg agaaaaggta ctgattatag aaataattat tctgccttta
     6421 aatctatggg gggaggagta gaattagact gtatacatga attggattat attataaacc
     6481 tatttggttt ccctaaatca gtaaaaaaac agtttggtaa atattcgaat cttgaaatta
     6541 acagtaatga tttagcaaca tatatattga gatacgatga taaaattata gaaattcatt
     6601 tagattattt tggaagagtt cctacaagaa aatttcaatt gtttacttta gatgaatgta
     6661 tcaatgttga cttattaaat aataaaataa gttttgaaga aaacattgtt aaagaaatgc
     6721 ctgaatctgc taatgatatg tatgagaagg agttgaaata tttctttgaa aacgtagtaa
     6781 caggcaaaga aaactggaat gatttatatc atgcaaacga agtattgaaa atagctgagg
     6841 agaaataaat ggaaatttta tttactattt gtggtagagc tgggtctaag gggtttaaaa
     6901 ataaaaattt gaaatatttg gcaggaaagc ccctagtcca tttcgcttta gcaatgatag
     6961 atatctatca acaaaaacat cctgaacata attgcacgac ttgtttgaat acggacagta
     7021 atgagttagt agaattgacg actaagttaa atccgtctgt gaaaattgta gaacgtaaac
     7081 ctgaattagc tacagatact gtagggaaaa ttgaagtgat acgtgattca ttcttacaac
     7141 tagaaagaga agataatata tttgatttaa ttatagattt agatttgact tcacctttac
     7201 gtgaagtttc taatcttgaa gaattaatag atgaacatct aaaaaattta gataaagacg
     7261 tctacttttc agttgtttct gcaagacgta atccctattt taatatggta aaaagaattg
     7321 aaaatgaagt tactatagtt aataaatcaa gatacacatc taggcaacag acacctgaag
     7381 tttttgaaat gaatgcctca atgtattctt ttaaacctga atttctaaaa aaaagtaatt
     7441 atatttttga tgggcaatgt gggataataa aaatgagaga ttatctaatt ttagatattg
     7501 atagtgaaga agattttaaa tggttagaat ttttgtttcc taaattttta gaagattctt
     7561 cggagcttca agatatctat aattatttat atcggaggta aaaggatgag ctgttatctt
     7621 atcaccggtg gggctggctt cattggttct accttagcca atcatttagg agaagaacat
     7681 gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag aaaatcttga tatgacgaaa
     7741 aatatcactt ttattgaggg agatgtcgct gagaaatcct tgatggaaga gatcatgaaa
     7801 gctaataaat ttgattatat ctttcattta gcagcagttg ctagtgtggc agattcagtt
     7861 gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg tattaatgat tttagaattg
     7921 atccgcaagt atcaacctga tttaaaacgg atcgtgttta gttcttcagc ggctgtttat
     7981 ggggatgaac cgactttacc aaaaaaagaa gagtccgtga ttcgcccatt gacgccttat
     8041 gcggttgata aattcgctgc agaacaatat gtgctagatt attgtcattt atatgatgta
     8101 cccggtagcg cagttcgttt tttcaatgtc tatggtccaa atcaaaatcc gaattctcca
     8161 tacagtggtg tgatttctat tctcgtagat cgctataaga aacaaatcgt aggagaagca
     8221 acttcattta cattatttgg tgatggaagc cagtctagag actttgttta tatagatgat
     8281 gtaatccaag cattactttt agttgcgaat gaggaagctg cactggggaa acaattcaat
     8341 gttggaacgg gtaaagcaac cacacttttg gaattgattc atgcaatcaa tgaaattctc
     8401 ggtgtagaat tgacacttga atacaaagaa gagcgttcgg gtgatatcca tgactctcta
     8461 gcagatatta ctaagatcca gtcaattggc tatcaaccga aatatggtat tttaagtgga
     8521 atgactaatt atttgaaaac tgaaatcaat taaaacatac taagataaga ttagagggta
     8581 aattcaggct ctttgtcaat aaggactgat gagttttgta aaatcaaatc tgggatagaa
//
LOCUS       DANJLF010000093         8612 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000093 DANJLF010000000
VERSION     DANJLF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            44..436
                     /locus_tag="PWT25_002463"
     CDS             44..436
                     /locus_tag="PWT25_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022500.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(609..791)
                     /locus_tag="PWT25_002464"
                     /pseudo
     CDS             complement(609..791)
                     /locus_tag="PWT25_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            778..975
                     /locus_tag="PWT25_002465"
     CDS             778..975
                     /locus_tag="PWT25_002465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022501.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1018..1593)
                     /locus_tag="PWT25_002466"
     CDS             complement(1018..1593)
                     /locus_tag="PWT25_002466"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL1022502.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1748..2274)
                     /locus_tag="PWT25_002467"
                     /pseudo
     CDS             complement(1748..2274)
                     /locus_tag="PWT25_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2731..4242
                     /gene="gadC"
                     /locus_tag="PWT25_002468"
     CDS             2731..4242
                     /gene="gadC"
                     /locus_tag="PWT25_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HDL1022503.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4255..5655
                     /locus_tag="PWT25_002469"
     CDS             4255..5655
                     /locus_tag="PWT25_002469"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDL1022504.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5820..7260)
                     /locus_tag="PWT25_002470"
                     /pseudo
     CDS             complement(5820..7260)
                     /locus_tag="PWT25_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7653..8246
                     /locus_tag="PWT25_002471"
                     /pseudo
     CDS             7653..8246
                     /locus_tag="PWT25_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
     gene            complement(8230..>8612)
                     /locus_tag="PWT25_002472"
     CDS             complement(8230..>8612)
                     /locus_tag="PWT25_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL1022505.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         2731 a         1441 c         1559 g         2881 t
ORIGIN      
        1 ttcattttac tttaactaat aatgtttatt tggagggatt attatgttag atgatagatt
       61 gacgtgttct aattgtggta cattatttta ctatgaggaa gttagaaata ttgtaaaaca
      121 tagaaaaaaa gaaatgccta tacgttgtcc aaattgcaat gaagttatta agcgaaagtt
      181 gtctcatggt tattttgtca gctacgaact atatgaagaa agtaaaataa atctgaaaaa
      241 aaaaccaaaa tactataaaa atttgaaaac tggagctatt ataactgaaa aagaatttaa
      301 aaatttagtg actgaagaag aaagcaggtt ttataatttg tctggtaata tttgccctgg
      361 atttatggat cagattattg aaaatgagtc aattgaatca attgaaaact atgttcctgt
      421 tgacttacca aaatagaaaa aataatcctg tttttacagt gaattatttt aattttgatt
      481 ttcaataaaa atatattaca ataataatcg taatagctaa tgaaacaatt tctcaatctt
      541 tttaccactc caaacaggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa
      601 ataatgggtt actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta
      661 tggcttctat ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct
      721 agtgattctt cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttattg
      781 aaagattcga ttgtagaacc agaaggaatg gagaacactt ttgaacttta caacaaaact
      841 actgttgata aattaattcc aattttagtt ccaactgatt taaatggtca tgatgtttta
      901 cttcgtttta acggattttt aggtgaggat tatagatacc taaaaatttc tgatcctcaa
      961 cctttaacag aataataaaa aacgtgtaat caattatttg attacacgtt ttttatttca
     1021 atcttcgttg tagtagtggt tgcgacatac cgaaaggtaa tcttcattac ctcctactac
     1081 aatctgttca cctgaataaa ctggaatacc atcaacaata cgagcattca ttacagcctt
     1141 tttattgcaa aaatggcaaa tagttttaat ttcctcaatt ttatcagcaa atgtcaataa
     1201 tgcttcggat cccttgaaga gattattttg aaaatcattc ttcaatccat agcaaattac
     1261 tggtacttcc tcattcatta cgataagtga tagatcaata atattctctc tacttaaaaa
     1321 ttgagactca tcgaccaaaa tacaatcaac ttttccgtac tgatcaataa tctccactaa
     1381 ttctccgtac acatccattt cagaagtaat tgcaatagct ggtttcgaaa cacctatacg
     1441 actctcaact tttccaaatc ctgatcgagt atctattgcg cttgtcatta tgataactct
     1501 cttcccttgt tcttcataat tatgagccac tttcagtagc tcaatactct ttccactatt
     1561 cattgttcca tatctgtaaa ataattgtgc cattaaattt attcccttct tttacaattc
     1621 aattattcct taattccatc tgcttttatg cagctgctcg tttattcctt gatatgtcta
     1681 taacatctac tatccctaaa accaatttat aagccattag agatggtatt cctgaacatc
     1741 tctcatattg ttttttgagt ggtatatcat ctgaaaaact gaaggaatca tgaaaatagc
     1801 gacatactta tcatttagag ttctaacttt aaatgcattt acttcttcag tcagcacttt
     1861 agtcatgttg gacatggttt ggggcgtgta gtgatgccca tacattttct caatcaagtc
     1921 tgcgatttcc gacatggtaa taccttttcg gaagaggtga atgacggtct cctctaatgt
     1981 gtcattcgtc cgtctataag caggaacagt ctgttgcttg aactcgccat tgcggtcgcg
     2041 cggaatctgg agatgaagtt ccccatactc ggtcttgacc gtacggtcat aggagccgtt
     2101 acgagaatta cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga ggaaagccgt
     2161 taactccgtt ttgagaagcg tattgacagc tttttccaaa tggacacgga ataatccatt
     2221 caaatcgcct ttgttcgcta gagtctttag gatttctgta gtaaaatcgt tcatatggaa
     2281 gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttcctttttt
     2341 ctatttacac aaaatatttt acactctcaa caaaagtatc ttcgattaat acttgctcaa
     2401 gttatgtgca gctacgaaaa agtaggtacc gaggtacttt ctttttcgca gctttttttg
     2461 gacttatgag aagtacattc gctggaagaa tacgatataa aacgagcttt ttcactaaag
     2521 atattaatag agtgaaagaa acaagctttt gttgtcagtt ttttcacttt tctaaaaatg
     2581 aaaataaaaa attctggaag tggagaagaa ttttgcttgc tatactttgt cttgtagaaa
     2641 agaggtagac aaaataatgc aagctcaaat aagcgatttt ccataacctt ggtaggaatg
     2701 acaaattaaa taggaaaggg aagtaataat atgaataaaa aaaagttatc tctattcggt
     2761 ttttttgccc tgacagcttc catggttttg actgtttatg agtatccgac ctttgcaaca
     2821 tcacaattac atttggtttt ctttcttctt ttgggaggta ttttatggtt tctaccagtt
     2881 gctttatgtg ctgcagaaat ggcaacagtt gaaggatgga aagatggtgg gattttcagt
     2941 tgggttagtc aaactttagg agaacgtttt ggttttgcag ctattttttt ccaatggttt
     3001 caaattacag taggttttat tacaatgatc tacttcatac taggagcgct gtcgtatgta
     3061 attaattttc cagagcttaa tacagatcca tttatcaagt ttattggtgt attagtcatt
     3121 ttctgggtac tgactttttc acagttgggc gggaccaatc gtaccgctaa aattgctaaa
     3181 gcaggatttg tcattggaat tgttattcct tcgatcattc tctttggttt agcagcagcc
     3241 tactttatcg gcggtaatcc gattcaaatg gctattaatg cgaaagcttt tattcctgac
     3301 ttttctcagc tttcaacttt agtagtattt gtatctttta tattggctta tatgggcgta
     3361 gaagcttcag catcacatat taatgagctg gaaaatcccc aaagaaacta cccattggca
     3421 atgtttttac tagtgatttt ggccatcgcc ttagatgcaa tcggcggttt ctcagtagca
     3481 gcggttattc cacaacatga cttgtctctc agtgctggtg taatccaaac atttgaaacg
     3541 cttattttac attttaacag tcatttacat tggttggtaa aagtgattgc tctcatgatt
     3601 gcctgtggcg ttatgggtga agttagttct tgggtagttg gtccatcacg agggatgttt
     3661 gcagctgctc agcgaggtct gttaccagtt ttcttccgga aaactaataa acaagacgtt
     3721 ccagttccgt tgattatgat acaaggaatt atcgttacta tttgggcagc agtcttaact
     3781 tttggtggag gaggcaacaa tttatcattc ttagtagcaa tctccttaac tgttgtcatt
     3841 tatctcgtag gatatttact atttttccta ggttactttg tattagtttt taagaaaaaa
     3901 gacttaaaac gaacttacaa tgtaccagga aaaactgttg gcaaaacgat tatcggtgga
     3961 attggtttta ttttatcagt gttcgcattg cttatctctt ttgtaccacc agcttcaatt
     4021 gcagctagtg agcaccgaac ataccaaatc atactactta ttagctttgt agtgactgtt
     4081 cttttacctt ttatcattta tgaagtacat gataaaagtg ctcacacaac tattgaggaa
     4141 cctactcacg tgaaagctga agacgttaat cccgctgttt atccagctgc tagaggagag
     4201 caccacgtta caaaaaatga tgaacatatt ttaaaacatt aggaggaatt cattatgtta
     4261 tacggaaaag ataatcaaga agaaaaaaac tatttggaac caatttttgg ctctgcaagt
     4321 gaggatgttg acttgcctaa atataagtta aacaaagaat ccattgaacc acgaattgct
     4381 tatcaattag tacaagacga gatgttggat gaaggaaatg cgcgattaaa cttagctact
     4441 ttttgtcaaa cgtatatgga acctgaagca gtgaaattga tgacccaaac gttagaaaaa
     4501 aatgcaattg ataaatcaga atacccacga acaacggaaa ttgaaaaccg ctgtgtaaat
     4561 atgattgctg atttatggca tgctccaaat aatgaaaaat tcatgggaac ttcaacgatc
     4621 ggctcttcag aagcctgcat gctgggtggt atggccatga aatttgcttg gcgtaaacgt
     4681 gctgaaaaat taggtcttga tattcaagca aaaaaaccta acctggtgat ctcttctggt
     4741 taccaagttt gttgggaaaa attctgtgta tattgggatg tggaactgag agaagtccca
     4801 atggatgaaa aacatatgtc aattaatcta gatactgtca tggattatgt ggatgagtac
     4861 acaattggta ttgtaggtat tatgggtatt acttacactg gtcgttatga tgatatcaag
     4921 ggtctgaatg atttagttga agctcacaat aaacaaactg actataaagt atacattcat
     4981 gttgacgctg catcgggtgg cttttatgca ccatttactg aacctgatct agtttgggat
     5041 tttcaattga aaaatgttat ctcaattaat tcttcaggtc acaaatatgg tttggtatat
     5101 ccaggtgtgg gttgggtctt atggcgtgac caacaatact taccagaaga attagtattt
     5161 aaagttagtt acttaggtgg agaaatgcca actatggcta tcaacttctc tcatagtgca
     5221 gcacaactaa ttgggcaata ctacaacttt gtacgctatg gctttgatgg ttatcgtgat
     5281 attcaccaaa gaactcatga tgttgctgtt tatttagcca aagagatcga aaaaactggt
     5341 atttttgaaa tcattaatga tggatcagaa ttgccagttg tgtgctataa gctgaaagaa
     5401 gatcccaatc gcgaatggac actatatgat ttatctgatc gtctgttaat gaagggatgg
     5461 caagtcccag cctacccatt acctaaagac ttggatcaat taattattca acgcttagtt
     5521 gttcgagcag actttggaat gaacatggct ggtgattatg tacaagatat gaaccaagca
     5581 attgaagagt tgaataaagc tcatattgtt tatcataaaa aacaggatgt gaaaacgtac
     5641 ggctttactc actaaaattc ttcagagaaa tataaaagaa gttaattaac taataaaagg
     5701 agttgtgatt accacacagc tccttaaatc tatttgaata aatatatgat attcatttgt
     5761 tacactctca atcttagctg ttgttctatt catcattact cttattgtaa gtagttcagt
     5821 caaatttgct tgttcaattg atcgataaca gtatgaattg tttgaaaagt aatcggatat
     5881 ataattgttt tctcactacc taagccttct tggtataaag aactatcgca aagtataatc
     5941 cgctgagtac aaaagaaagt gtcgtctact atttctctaa gttgtttaca aatgatatta
     6001 ctaatagaaa agtgttctcc caaaatgtct tctaactgtt gtcgatagta taaataccta
     6061 aagtctgacg gagctactat gaacacttca attggagatt tccgtttatg ctcatttaaa
     6121 agacttaaac taatggttag tttacgaata gaattctcat tccaacgcag aaataaattg
     6181 ttttgttttc gccattgttc aagaatttca tgaacttcta agtacaaagc gcgttgctct
     6241 tcatttaaca agtaagtctt atcaggttga aatatctgaa tatcagccca aagtgagcgg
     6301 ataaacggga gaagtgcttt atcaaaaagt atctgatttt cttcagatat attcagagat
     6361 aagataagtt tttgactgag ctcaatcacc aaagggttat ctgctacatg gttttgataa
     6421 acaacgttca agtctttttg taacaattca aagttatcgc atagataatt tctagagttg
     6481 aatagaaagt agatataaag gcattcatct tcgtcatgac ataaagagaa agaaagttgc
     6541 tctaatcctt tctgtattag ctgaaacaag ggagtatgtc gggcaactct tttttcttcc
     6601 tgactaaaat atattgggtt agtcttagat ctctgcaaac cgatagcgat acctaataaa
     6661 ataaattgtt tttcaactgt agggtaactt cttgaaaaaa aatgtttttc tacataatca
     6721 atcaattgtt gaccgctttg aataattgat ttatttagct ggatcaactc tgtccgattt
     6781 gtctcataga ttatcgttag taacaaactt cgataatcct tttcaggaat ttcaaaatgg
     6841 ccatcagcat ttaagtactg catttgttta aaaaaattaa tcaactcatt tcgtaagttg
     6901 taaactttgc ttaacgaaat gaaggtagta tcagctatat tactccaatt gtgttcccct
     6961 gataaataat gtcctagtac attgaaaaag tcagagcgct gatagatgag ttgagtgagt
     7021 gccaacctcg aaaacgaaga gtcgaatgcg actgtaaagc tacttctaga tttctccact
     7081 gaaataattt gctgatggag tatgtcggta atgcattcca agtcgttagt gacagacagt
     7141 gagtgggcaa acgttcaatt gatctgctat ttcgcgaatc tgaatggtcc ctttttcgtg
     7201 taattgctca caaatgtaaa cttgacgaaa aatacttttc tccagatagg tttccaacat
     7261 agtactcctc ctagtcattt tatacataac tattataact ctaccaatcg gaatagtcct
     7321 gtaatatatt gaatcaaaaa tataaattat aaaataatta gatggggtat ttggtttaag
     7381 tatcccttat atagtaataa atttataggt gtgattctcg aattaatacc cgaaaatgtg
     7441 agagaatttt tatggatttt ttaggataca aattaattat ttttgctttt atcctgatta
     7501 ccagtccagt actgtcggta tcccattttg cagcaaaatc atgttgataa agtgacaaga
     7561 agactgatga gatatattat ccaaattgag aagataatta atcggattct tgagtatacc
     7621 ctacttttta agaaagtagg gtatacaagc gtatgaataa agacataact ttccctgtga
     7681 tggacgttcg gcttgttcca attaccaaaa tacaggcgaa cgattataat cccaatcgag
     7741 tagcgtctcc ggaaatgaac ttattggagc tttctatcat tgaagatggc tttactcaac
     7801 cattagtttg ttattacaag gaaaaagaag agaagtatat cttggttgat gtgcttcata
     7861 ggtatcgtgt tgctaaggaa agactcaact taaaagttgt accggtaaca gttattgata
     7921 aacccatata agaacgtatg gcttcaacaa ttcgacacaa tcgtgcaaga gggacccatg
     7981 aaataagagg aatggaaatt gtcatccaaa gattagtaaa aaatggatgg tcagacaaga
     8041 gaatatccaa agaattaggc atggacattg aagaaatttt tcgctttaag caagtgtctg
     8101 gtggaatggc aacagttttt ttgacaaatt ttataaggtg cagaacttct ttccgtatgc
     8161 tattccgatt gtccttgaca atgagcctcc tcggatgtga cgatcttcgg caggagctaa
     8221 cagttaaagt tagactgcct cgctagcgtt agcacatccg agctcattat caaggattcg
     8281 ctgcgccaat ctctggttac ggtaaccaac cggtgtctcg tagccaagtg taccgtgcaa
     8341 ccgaaggtgg ttccaccaat ttacatagtc aaataactcc aaatccaatt gttgtaaggt
     8401 ttcaaatgtg tattgataga caaattctac tttcaacgac ttataagttg attcagctac
     8461 ggcattatca aaaggacagc ctttatgact caatgatcga ttgatgtcaa aagttgttaa
     8521 taattcatca atagcttggt tatcaaactc ttttccacga tcagtatgaa aaatctcaac
     8581 ctctgtcaga ggttgtttga tacggctaaa tg
//
LOCUS       DANJLF010000094         8494 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000094 DANJLF010000000
VERSION     DANJLF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..173)
                     /locus_tag="PWT25_002473"
     CDS             complement(<1..173)
                     /locus_tag="PWT25_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1022506.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PWT25_002474"
     CDS             complement(593..1714)
                     /locus_tag="PWT25_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL1022507.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PWT25_002475"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PWT25_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL1022508.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PWT25_002476"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PWT25_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL1022509.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PWT25_002477"
     CDS             complement(4304..5215)
                     /locus_tag="PWT25_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1022510.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PWT25_002478"
     CDS             complement(5232..6239)
                     /locus_tag="PWT25_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL1022511.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PWT25_002479"
     CDS             complement(6236..8365)
                     /locus_tag="PWT25_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1022512.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="PWT25_002480"
     CDS             complement(8370..>8494)
                     /locus_tag="PWT25_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1022513.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DANJLF010000095         7885 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000095 DANJLF010000000
VERSION     DANJLF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..440
                     /locus_tag="PWT25_002481"
     CDS             <1..440
                     /locus_tag="PWT25_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022514.1"
                     /translation="KTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVLLSTTLKG
                     KFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDR
                     YVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(566..1243)
                     /gene="nth"
                     /locus_tag="PWT25_002482"
     CDS             complement(566..1243)
                     /gene="nth"
                     /locus_tag="PWT25_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL1022515.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1250..1948)
                     /locus_tag="PWT25_002483"
     CDS             complement(1250..1948)
                     /locus_tag="PWT25_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL1022516.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2216..3400
                     /locus_tag="PWT25_002484"
     CDS             2216..3400
                     /locus_tag="PWT25_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL1022517.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3406..3849)
                     /locus_tag="PWT25_002485"
     CDS             complement(3406..3849)
                     /locus_tag="PWT25_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1022518.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4075..4362)
                     /locus_tag="PWT25_002486"
     CDS             complement(4075..4362)
                     /locus_tag="PWT25_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022519.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4533..5885)
                     /locus_tag="PWT25_002487"
     CDS             complement(4533..5885)
                     /locus_tag="PWT25_002487"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL1022520.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6158..6604)
                     /locus_tag="PWT25_002488"
     CDS             complement(6158..6604)
                     /locus_tag="PWT25_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL1022521.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6840..7766)
                     /gene="cysK"
                     /locus_tag="PWT25_002489"
     CDS             complement(6840..7766)
                     /gene="cysK"
                     /locus_tag="PWT25_002489"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL1022522.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2143 a         1598 c         1278 g         2866 t
ORIGIN      
        1 cgaaaacaat acctcgttat tccaagaata aaaaagctga aaaattttgt ttattattaa
       61 ataaatttac tttaattgct cctatccttg catttattat tttttctgta cttttaagca
      121 ctactcttaa aggaaaattt atggaaagaa gctctcatgc aatgattctc acttttttat
      181 ggttgttgtt caccagaatc tatatttttc tgatgagcct gaaaccgccc aaatccatta
      241 gtttatgttt agttataaat ggtatttttt tattatcact tatcattttt attacacctt
      301 tagatagata cgtcacttat ttatataatc ctttggaata ctggacttat tttataggta
      361 tattggaagg cattattttc tacatcgggt attttcccaa taaaaataac aacttttatt
      421 tttatcgcaa taaattgtaa taagttacca tcctagctac tgagtatgta catttacttt
      481 ttcatgcaaa aaagataaac gaaacagctt ttctccataa taacaagata attcatacat
      541 ttatttgcaa ttaaagaaac cacgtctaca ggcgtggttt cttttttagc gccttttctt
      601 ttagacgcat ccgatttttc ccatcttgac atattgatag caaaggacat acctcgcact
      661 tgggatttct tgccgtgcaa tgatatctac caaaaaaaat cagtgtatga tgtgtcttta
      721 cccataattc ttgtggtact ttccgcatca gtgtttcttc tacttccatg accgtagcat
      781 ctagtttgca gatcctcagg cgttttgaga cacgttccac gtgggtatct actgcgattg
      841 ctggtatccc gaatgcatcc cccaatacga catttgctgt ctttctgcct acaccaggaa
      901 gagacatcaa ttcttctctt gacgttggca cttgcccatc gaaacgttca atcagctgct
      961 gtgcacacgc tttgatgttt tttgctttat ttcgatacaa accaatcgtt tttattttaa
     1021 gaatgatttc atcgattgaa gcttctgcta acgcatcggg tgtcggaaaa gaggcaaaca
     1081 aatctggcgt tgctttattc actgatacat ctgtcgcctg ggcacttaag atcactgcaa
     1141 tcaataactc aaatggatta ttgtgcttga gttctccatg tgcttcggga aacattccat
     1201 acatcgtttc tagagcttcc attgtttttt gtttgcttaa catattccgc tactcctcca
     1261 accaattttt cagtgagact ttcggcaatt cttcttgttg agctgactgt tctacttctc
     1321 tttgaagaag ctgctgtttt cttcgttttt ggtcttcttc cacttgttgt tttgtacgta
     1381 gattttttct ttcccacgat agcaaaatgc ggtcaacgta attaaaacta taagcctgat
     1441 ttaatacggc ttcccgcagt gctagcttca aaatttccgg ctgataatgg tcttcttcta
     1501 gccattgtcc gatccgctgg aactcgatcg aagaaagcgg gcgtccaaat tcttgttcaa
     1561 ataattgata cactgactgg atttcttttt cttgtgtttt ttcttcgtat tttttttctt
     1621 gtgtttttaa atactcacct aatagatcat agatggggta aagattatat cgatctgctt
     1681 ttttaccgtt gacaatcgtc gtttctatct tgataaatcc atttgttacg agacgatcca
     1741 gtatttgaaa aatctgatct tgtttgattc ccatatccat agcaatcatc tgcaaatcag
     1801 ggaaagtatc tccttcaagc tgtgccatat gcaactgtag aacaaataaa aattcttcag
     1861 attgtagacc gatcctatga taatttttaa gtaaaagatt agatatgact gtttgtccag
     1921 cgcttaagta attttctaaa ttcaacatga tttctcctcc gctcaatcac agtatacgta
     1981 aaaaaaacat gggaagcaag aacaggtttc ttttctattt acttcactga tcggtcagat
     2041 ttacaatgtc gtctacttga tttttgtttg ttcatgtttt tgatgcgtat cttcactttt
     2101 cggaataaat aaattaagta gatttcgctg ttccttagaa tgttgacaag gaaacaaaat
     2161 tactatacac tatttttgtt tccaagttaa cattattgca taaaggagtt tttacatgta
     2221 cagtttcaca aataaacatt ctaaagtttt tggatacgtc tctgtcttat tcactggatt
     2281 aggatttact atcgtcagtc ctgtacttcc gtttttaacg ctgccttatt ctcactccat
     2341 acatcagcaa gctttctata tcactttgct catgtctgtt tatgctctag ctgccttctt
     2401 atctgccccc attctcggta gcttaagtga tcattttggg cgtcatccga tccttattat
     2461 cagtttatta ggatcaagta tcggatattt gctttttggt ttaggaaatt cgatatggat
     2521 gttgttcctt ggtcgtatca ttgaaggatt gacggcagga gaaatctcca ccttatatgc
     2581 ttactttgcc gacatcacgg aacctaatga aagaaccaaa gtttttggat ggatgggtgc
     2641 tttagtcggt ataggaacaa cgctgggtcc tattataggg ggtctattag ctgagttggg
     2701 gaattctgtt cctatattta ttggagcact ctttactttc ttaaatgccg tttatggcta
     2761 cacatttatg ctagaaagcc ttccaataaa aaaacgttca gtagatttaa gtttctccca
     2821 tgtaagacct tttcaccaat tacaacaatt attcaagatt tcatccgtta ttcctttatt
     2881 gacagcaggt tttgctgttt ggttggcagc tggctctctt caatctatct tttctcaatt
     2941 ttcgattgac gcctttcagt ggaaggctgg gttggttggt ttaagcttct ctttgattgg
     3001 aatcttggat actgtctcgc aattgttcat tatgcctcga ttgctcaaaa aattttcaga
     3061 acagcagatc acaagaatag ggatgttcag tgaaatcctc gcctatctat tcatcacatt
     3121 atctggaatt cttctactgc cgattttgtt tttatttggg attatctgtt atggtttcgg
     3181 tgattctatt tttactcctg tattcaatgg ccaactatcc aattctgtct cagataacca
     3241 gcaaggtctt gtaatgggcg gtacccaaag tatacaatct ctatcgcgcg tcatcggccc
     3301 gctaatcgca ggacagcttt atgctgcagc accatttctt ccgacggtat tcggattttt
     3361 gcttatgatt tttgcctatt atcactacaa taaaaaatag tagcttcatt tgctacttcg
     3421 tataaattct ctgtatcgac cgataaaacg aaatactgtg tcaaattctt cttttgacat
     3481 attttcaaat acagtagcat ctcgctgtac ccatttttga tggagttgct cgtgtctatt
     3541 ccttattttt tgtccgattg gcgtcaattg aaaatagatt tcttttttat tctcttcgct
     3601 ttgataagtg tttatgatcc ctctagctat cagttttttt gttagcttac tgatggctcc
     3661 tctagtcatg taactagcat tcgctaattt tgtcacgtta ggatcaggga ttttcccgat
     3721 atgttcaatc acttctagtt catttagagt aaatcctgca aattcttttt ccatttctgg
     3781 tttattacga tgaataatct cattagctaa atccagatat tcttctatta gcagttgtct
     3841 tttatccatt tctacacctt ctttttcttt cattatattg tttttttgtt tcctattcaa
     3901 caaagataaa aatattttgt aggaaaaccc aatctgaaac caagaaaaaa aggggttgac
     3961 aaagcttgtc ataacccctt tttctgatta aaagatagta aagagacagt catcggtaat
     4021 tgtgatgatt ttttgtcatc acttaaggat gagcggcctt ttcaaaaact gtctttaaag
     4081 atcatcatca gtttctagca catatggctg accttcatat gtgtactctg tattcaatgt
     4141 gccgtcattt tcatctgagt agatgcgtct actgaaagga aaacgatgag aaacattatc
     4201 tttggcgtca tttgctggca tcgtgtaata ttcctcttgc gtttcattca actgagagag
     4261 tacttgatct gccaactcat caatattgat cgttaatttc ttcgaagcac ttttataatc
     4321 ttctaaaaat gcatcttgtt ggacaaaata ttctttattc atcttctttc tcacctttct
     4381 tttcaaaatc aaataaagct gcttgttctt ttatgtaaca gctttatctt tgctgcttat
     4441 tgtgacaaaa aattcgaaga aagacaacta attgtacttt tcacaaaaaa agacagaggg
     4501 tcgtgacaaa attgtgtcac aacccttttt ctttattgtg cagtttcgat ttcgcctagc
     4561 gctgcttgtg caagtaggtt caagtaattc catgggcggt caaaatgtgg ttggaagaag
     4621 aaatctgaaa gtgcgagatc ttctacagtc attttatttt gaacagctaa agacatggta
     4681 tttgctgatt gtgtaatgtc gtatttggac atgaactgag cacctacgat acgattcgtt
     4741 cctttttcat agactagctc catcattact ttttcagtag ttggcatgaa ctctggacga
     4801 taattatctt caaatacagt cgcttggact tctagatcgt tcaaagttgc gctttctttg
     4861 gtcacacctg tcgaaccgat cgtccagcca aataggtaaa gtcctgaagt tccttgagta
     4921 ccacgatacg ccattttatg atctgtcaag ttatttccca caagcaaacc ttgacgaacg
     4981 gcatttgttg ccaaaggaat atagttttgc gtgccacttg gattgtagtt gactactgca
     5041 gagtctccag ctgcaaaaat gtctggatta cttgtctgca tgtattcatt cactttgatt
     5101 gccccgtttg gcagtgtatc tacttttcct tcgatcagtt ttgtatttgg acggaagccc
     5161 acacataaaa tgaccatatc tgtatcaaat gtatctgttg gtgtagctac ttgtttcact
     5221 tttccttctt catctgtgat aaattcagta acattttcgc ctagcgcaag agtaacaccg
     5281 cggtctgtca attctttttc taagatatct gtgaatggtt tatctagata tttattcaag
     5341 atgcgatcta atccatcaat caatgtcact tgttttccag attcgacaaa agcttctact
     5401 aattcaatac cgatatatcc accaccaaca attactacac gttttgcttc tttcgcttgt
     5461 gcaataattt catttgcttg attgtagttt ttgcatagga gtacattttt cgcatcgatt
     5521 ccactaatcg gtgggataat aggccatgaa ccagttgtca tcactaattt atcataagaa
     5581 accgtttctg tttctcctgt tttcagatct tttgccgtta ctgttttatt ttctgtatcg
     5641 acgtctgtta catcgtgttc cattttaact gttgctccta atgaagataa ttcttcagga
     5701 tttgagtaga acaagcctgc tgggtccttt actactccgc cgacataaag tgcgataccg
     5761 caagataaga aagaaatatt gtcatttctt tcatatactg tcacttctgc ttctggatga
     5821 ttttttaaaa tactttttac agctgcagta cctgcatgag tacagccaat tactactact
     5881 ttcatttatg gtccctccga ttaacatttt gtccttcgac aaaactacta taacagaaat
     5941 aaaacggata caacagttta atgcttgtga attcacaaag gtttgtttgt agtttcattc
     6001 cttttcaagc ataagaaccc aatatctaca agattcttat tttaataata ttcactttta
     6061 tatctaagat tgtgaataaa agacaaaaaa ataaaaaagc tagcgttttt tttacacagc
     6121 tagctttttt taggtataac acttgtagaa actgttttta catttgcgcc ttttttgctg
     6181 actctttaca atccggacag agtccataca attccaaccg atgttcatta atctcaaaat
     6241 cagtcagtct gcttgcagcc atttctatat cttcaagccc tgggtaatga aaatcaacaa
     6301 tcttaccgca gttttcacaa attacatgat aatgacgctt tgaactgaaa tcaaatcggc
     6361 tagaagcatc gccgtacttc atttcttgaa caaatccaat cgaagtaaat aagcgaaggt
     6421 tattgtaaac agtcgcaaca ctcatatttg gaaaccgatg ttcaagtgct ttataaatct
     6481 catcagccgt tggatgggaa tgatgttcaa tcaaatattc taaaaccgcg taccgttgag
     6541 gtgtgattcg aatgttctct ttttttaact tttcgataac ctcttcgact aacttatttt
     6601 ccatgtaaac accccacgtt gttattctcg tcttatcata ctatttttac attttaaaat
     6661 ccacttaaca tgaaaagaaa attcaatttt attgtatttt aatgaaaaaa gacgtaaaac
     6721 agtcgcgttc actattctac gtctctattc aaataatttt tctcatcata aatttttgtg
     6781 tgttcttttc atctttaaaa gttttttaca taaacctatc tggagaaaaa ccaagtgcct
     6841 tatatctctt ggtaaagggc tgtagataaa taacgttcac cattgtcggg aaccacagct
     6901 aataccttct tgccttttcc taattctttt gcgactttta gcgcggcagc aattgcagcg
     6961 ccagatgaga tgcctactaa aatcccttct tttcgcccaa cttctctagc tgtttccatc
     7021 gcttcgtctc cactaataga aaggacttta tcatacacag aagtatccag tgttttagga
     7081 acaaaacctg taccgattcc ttggattttg tgaggtcccg cttctttccc ttctaaaata
     7141 gcagattctg ctggttctac cccgattaat tcgattttcg gatacacacg ttttaattct
     7201 cggccagcgc cagtgatcgt tcctcctgta ccgatacctg caacaaacgc gtccaagcca
     7261 tttactccaa atgcttgatg tatctcagga ccagtcgtct tctcatgcac aagaggattt
     7321 gcttcatttt caaattgtaa tggtaaaaag tagccgtttt cttttgccaa acgttctgct
     7381 tctctgattg atccagaaat accatccgca ccaggagtca aaatcaactc tgcgccgtac
     7441 cctttcatca ataaacgtct ttcgatactc atcgtctcag gcatcacgat gattacttta
     7501 tatccttttg caactcctac catcgacaat ccgattcctg tatttccaga agtaggttca
     7561 atgatagtat cacctggttt cagtagacca tcgtgttctg ctttttctat catacttaaa
     7621 gcaatacgat cttttacact tcctcctgga ttaaaaaact caagctttac aaaaacatct
     7681 gctgaatctt ctggtacgat tttatttaat ttgacgattg gcgttttgcc aatcaattcg
     7741 gtgactgagt tatagatagc tgtcatcttc aattcctcct tgtctttgct cttcttactt
     7801 atcataatac aatctgttca tttatggcga gtgattcgtc tttgactagc aaaaaaaaga
     7861 caaagatata ccatatatgg taatt
//
LOCUS       DANJLF010000096         7840 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000096 DANJLF010000000
VERSION     DANJLF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(21..995)
                     /locus_tag="PWT25_002490"
     CDS             complement(21..995)
                     /locus_tag="PWT25_002490"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL1022523.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="PWT25_002491"
     CDS             complement(1075..1950)
                     /locus_tag="PWT25_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL1022524.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="PWT25_002492"
     CDS             2080..3987
                     /locus_tag="PWT25_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL1022525.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="PWT25_002493"
     CDS             complement(4528..7806)
                     /locus_tag="PWT25_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL1022526.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2208 a         1659 c         1461 g         2512 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt
//
LOCUS       DANJLF010000097         7696 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000097 DANJLF010000000
VERSION     DANJLF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..178)
                     /locus_tag="PWT25_002494"
     CDS             complement(<1..178)
                     /locus_tag="PWT25_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL1022527.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(191..1294)
                     /locus_tag="PWT25_002495"
     CDS             complement(191..1294)
                     /locus_tag="PWT25_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL1022528.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1384..2340)
                     /locus_tag="PWT25_002496"
     CDS             complement(1384..2340)
                     /locus_tag="PWT25_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL1022529.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2621..4555
                     /locus_tag="PWT25_002497"
     CDS             2621..4555
                     /locus_tag="PWT25_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1022530.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5012..5428
                     /locus_tag="PWT25_002498"
     CDS             5012..5428
                     /locus_tag="PWT25_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL1022531.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5442..6008
                     /locus_tag="PWT25_002499"
     CDS             5442..6008
                     /locus_tag="PWT25_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL1022532.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6031..7017
                     /locus_tag="PWT25_002500"
     CDS             6031..7017
                     /locus_tag="PWT25_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL1022533.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7172..>7696)
                     /gene="scm"
                     /locus_tag="PWT25_002501"
     CDS             complement(7172..>7696)
                     /gene="scm"
                     /locus_tag="PWT25_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL1022534.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2203 a         1473 c         1591 g         2429 t
ORIGIN      
        1 tattgatagc ggtagtcgct aaatcaaaaa ctagcattcc aataaaaaat aaaaaagtgt
       61 ttagccaatg tatctcatga aaaaaagcga tagagaataa tacccctacc gcaaaaggaa
      121 atacactagc cacctttgtt cgtatttcaa ctacttctaa aaagattttt agtgtcatgg
      181 ttcgtactcc ttattttagt tcagaacggt cacccatcgt gtaatgggag tcctccgata
      241 atttgaaggt gtctttgatt ggatcggtca catatacttt atcgtctttt gtcacgaaga
      301 tcgcttccgt cccgttattc aattcgcttt cgacgtattc cagtcctttt ttcacaccca
      361 ttgaaaagac tgctgtagac aacccgtcac catcaattga tttatctgtt atgatcgtga
      421 cacttgcgat atcattgtcg aacggataac ccgtttttgg atcaaacaaa tgatggtatt
      481 ttttgccatc tacttctaaa taacgctcgt aaattccaga ggtaactaat gttttgttcc
      541 gttctttaat agaacctaaa acagatccgc gagccatgtt cggatcttgg atgccgactg
      601 tccagtcttg gttttcgcct cttgggctgt ggcctaagac atagacatta ccgcccaagt
      661 caacgatggc agtggtcact ccttgttttt tcaagacttt cactacttcg tccgtaatgt
      721 aacctttggc gattgcacct aaatcgatga tcattccttt ttcttttaga taaacagttt
      781 tttctttgtc atttagttcg atcttatgat aatccactaa tttcaatgcc tgatcgattt
      841 cttcttggga aggttttctg gcgtcatcaa aaccgattcg ccataattgt gtgattgcac
      901 caatcgccat atcgaatccg ccttgagaat cttggctata ttcgtaggcc cgcttgacta
      961 acgtgtagac gtcatcagaa acttttactg gtttgatccc agcttgctca ttcacttcat
     1021 cgatctcaga acctttttga ttgatcgtga ttttatcacc aagttctttt actcgatcga
     1081 aggcaggttt caatgctgat tctttgcctt catcatacac acggatacga acgtaagtcc
     1141 ctaacaagaa ctgttcatca gaatatggat ctttcaatat cttggtagct gctgaacttt
     1201 cagtggttgt tttatttgct gagttgctgc agccagttag caccataaaa gagatgccca
     1261 gtgtgattaa ggaaaatagt agttttttcc gcatcttttt cctctcttca ttttgaaagg
     1321 tgagacgact gcctaatctg gctatcgtcc caccctccaa cttttattag tatgattatg
     1381 tgattatttt tctgttacga tattatctac ttcgattgtt tgagtgtcgc ctttttcagc
     1441 tgcattcact aattgttgtg cgtacatgat gaatgaatgt gagctgtgtg tagcacctgt
     1501 aacaacttct acattagcag gtgagccatc ttcttcgccc attgctttga cgaattcgtc
     1561 gttcagagta gggatgtaag tttttggttc tgtaccgctt tttgctttca tattttcatt
     1621 atattctgta tcatcttgtt ttgatttacc gtctttgtcg atataatcaa agtttgattc
     1681 agttactttg ccgccagcaa ctgtcatttc gaattgcaca cggtagccgt tagagtagtt
     1741 cttttcttca agcttgtaag tgccgtcttt caaatcagca ccattgtcga tttcgattgt
     1801 atctgtgtta ccagcttgag cagcttggat caattgttgt gcgtagtttt ggaagctttc
     1861 agatgagtga gtagctccag ttacgacttc cacaccgctt gcgctttgtg cttttaagaa
     1921 ttggtcattc aactcaggaa tgtatttttc tgggtttgtg ccagattttg cttccatgtt
     1981 tttgttgtat tctgcatctt ttgtttttga ttcgccgttt tcattgacgt tatcatattt
     2041 tgattcagtg attttgccgt cttttacaac catttcaaag actgcacggt aaccatttga
     2101 ataatttttt tcttctaatt tatatgtgcc gtcttgcaaa tctccgccag ctacaacttt
     2161 tgcagatgat tctgttgtgc tttttgcagt cgaagattgt gctgtagatg attcagaaga
     2221 agagtttgag cttccgttgt catctgcgcc gcaagcgcct aacactagag ctgaaaatgc
     2281 taatacggca aatccagtta caaaacgttt tttcttcatt ttaatacccc accattccat
     2341 ttctagtcca caatttttct ttgtgattca ttatacattc taatcttttt agcctataag
     2401 tcaactattt ttgataaata acccatgtaa tatttcaaac gttttctttc agcaaatttc
     2461 gtgaaaaaac gctttatttt aaaatttgaa aaaaatccaa aaggagttag acaaaagaac
     2521 agaacatcta tataatatat ggagattgtg taataagtat caaactaaaa gaattatcat
     2581 atattcatta tatagatagt ctataaggag cggatgatca atggcaaagc aaaatattgt
     2641 cgttgtagga gcaggatatg ccggtgtttc agctacaaaa tacttggcaa agaaactgaa
     2701 aaaagaagat gtagcaatta ctctgatcga tcgtcattcg taccatacta tgatgaccga
     2761 gttgcatgaa gtagctggcg gacgtgtaga accagaagca atccaatacg atctgcagca
     2821 tttgtttgct cgtcaaaaga atgtacaact tgtcacagac actgtaatcg gtatcgataa
     2881 agaacgaaaa atcgtcaaga ccaaattagg ttcctatcct tttgatcagc tgatccttgg
     2941 aatgggcgga gagccgaatg actttggtac acctggtgtc aaagcacacg gattcaccct
     3001 ttggtcattt gaagattctg taaggattcg ggaacatatt gagaagacag tagcaaaagc
     3061 tgcattggaa ccagatgatg aactgcgtaa agccatgctg actttcgttg tctgtggttc
     3121 tggatttacc ggtattgaaa tgattgggga actgatcgac tggaaaaatc gcctagccaa
     3181 agattataaa ttagatccaa acgaaatcac actgatggtc gtagaggcaa tgccgacgat
     3241 cttgaatatg ttggatcgaa atgatgctgc aaaagctgaa cgctatttgc gtaagcacaa
     3301 cgtggaatta ttattgaatg caccaatcgt agaagtggct gctgatcata tcaaattgaa
     3361 agacggacgc aacattccga ctcacacact gatttggaca gctggtgtca aaggaacttc
     3421 tgacgctgct gactttggat tagaatcagc acgcggacag cggttgatcg ccaatgaata
     3481 tatgcaggca aaaggctacg aagataaaaa catctatatc attggtgatt tagtctttta
     3541 tgaagaattc ccaggaacac cgacaccgca gatcgttcaa gctgctgaac aaacaggaca
     3601 cacagccgca gcgaatatcg tggcttcgat caaaggcgga gaaaagcata aatttaaagg
     3661 aaactatcaa ggatttatgg tttctattgg tgccaaatgg ggggtagcta acctatttga
     3721 taagatccat ttaagcggat ttttagctat cgtaatgaaa catatcgtga atttgaaata
     3781 cttctttgat attcgttctg gttattatat gttccaatat ttaatgcatg agtttttcca
     3841 tatcaaagat gatcgcaacg tgactcgagg acattcttct cgttacggca atgtcttatg
     3901 gagtgtgcca cttcgtattt tctatggttt agtttggttc gttgaagctt ccaaaaaaat
     3961 cattgggaat ggtgaagtgt tcaaaccaag tacttggttt ggtgaaggat catggttcac
     4021 tggaaacagt gtgttccctt ggacatggga atacaatgta acagatgttg ccagtggtgc
     4081 ttctgctgct ggtgatgcaa ccacagctgc cagcggagca ggggcaagtg ccggagaagc
     4141 agcgactcaa gctgcacatt ttggtttgag ttatgcatac ggtgaagaac caatggctgt
     4201 gattgagaag tcaccagact ggttcacaaa gatcatgcaa gtgatcatgc caaacaatga
     4261 tgtagcaatg ttcttccaaa aattcatggt attctttgaa attgccttgg cattagcatt
     4321 gattgctgga ttattcacat ggctatgcag tgcgacaacg attgtattgg tgatcacgtt
     4381 ctgcttatca ggcatgtttt actgggtaaa catttggttc atttttgttg ctttcgcatt
     4441 gatgaacggt tccggacgtg cgattgggtt ggatcgctgg gtaattccat ggctacaacg
     4501 tacactaggt aattggtggt acggtagacc aagttcccag tacggcaaga aataaataga
     4561 taaagaacgt tatttgaatg caagatttca atagaaaggg gctgagacaa accgtcacag
     4621 cctccttttt atgtaaaatt aaaggaataa gatgcccgag ataagcctcc atgagcaaga
     4681 cacaggtgtc acaacctctg ttacaacctc ttgttataaa cggtgttcca gaagtagctt
     4741 cttcgaaaaa taagccgaaa tttcacaaaa acttgaaaag taattttcgt aaattccttc
     4801 ttatttcttg aagttgccca cttctgtcac aacctcttgt tataaacggt gttccacctg
     4861 ctgcttctcg gtaataagcc caaagaatta gaaaacatgg aaagctgttt tctattcttc
     4921 gttcttatta ctcaaagcaa gacacaggtg tcacaacctc ttgtttttat gctaaaattg
     4981 ggtgagttaa tctggaaaaa ggagaacaaa aatgagcctt aaagaatttg ctgaaaaaag
     5041 tctgctcaaa ccttgggatg tttttattat tttgcttttg actgttcttt cttttttacc
     5101 agtcatgatt tttggtcttc agcaaaacac cgcaacagca gaaaaagaag cggtactgcg
     5161 tgtggatggc acagaaatca aaacattccc tttgatggct ggagaagaga gcaacacata
     5221 tacttacaaa gatgaacacg gagattataa cttgatcgag atagatggcg atcgagtacg
     5281 gataaaagaa gcagattgcg atgaccagat ctgcgtacgt cgaggctggg caacgaaaaa
     5341 tggcgaaacc atcgtctgtt tgccacataa attagtgatc gaagtccgtt caacagatgg
     5401 aggtgacgaa gacagtctga tttattagac cttcacacac catgagtaaa ttacaaaaaa
     5461 tgatttatat ttcgctactt gttgcacaag gagtgatcat tggtttattg gaaaatatga
     5521 tcccttatcc tttttctttt gcgcctggtg caaaactagg cttagccaat ctaatcacga
     5581 tcgttgccat ttttaccatg aaaaaacaag acagcttctt actactttgg ctacgactat
     5641 tcctgactac tttgctaggc ggaacgattt ccacattcct ttatagtatg agtggtgctt
     5701 tgcttagtta ttttggaatg ttattggtca agcaattagg accaaaacgt gtcagtatca
     5761 tcggaattag tgcggccggc ggtttcatgc ataatgtcgg acagcttttg atggcttctt
     5821 tcattgcaca atcatggaca gtcatgttgt acttgccagt cctttcagtt ttagggattt
     5881 tatcagggat tgcaatcggg atcgcagcga attatctttt acgacatgtg cagacacttc
     5941 aacgattcca aaaagattat gagcagggaa ccaaacaaaa atggagccca atgtttacca
     6001 aaaaataatc gtcacacaag gagttttttt atgaagctac atccaatgtg ggaaaactat
     6061 cctgctttaa gtaaagaatt gacagaaaca ctaaaacgga tggaagaaac tgtccgcctg
     6121 aaaaacaaac ctgtcgaaca agccatcaaa gaaacgatcc atgccggcgg aaagctgttg
     6181 cgtcctgcct atcagctatt gttctcacaa tttggaccag agcaagatcg agacaaagcc
     6241 atcgctttgg cagcttctat cgaaatgctc catacggcca cactgatcca tgatgatatc
     6301 gttgatgaag cggatctgcg acgtggtcag cctaatatcc gtagtcagtt tggtaatgtc
     6361 gtttctgtct atgcaggaga ctatttattt gtctgctgtt ttaaactatt gtctgattac
     6421 tccacttcct tgaaaagtct ccaattgaat tctcgcagta tggaaaaagt attgaatggc
     6481 gaattagggc agatggatga tcgctataat tatgaacttt ctgtaaaaga atatttggaa
     6541 aatatttcag gtaagacagc tgaactgttc caattaagtt gttctgttgg cgcttttgaa
     6601 agtggaacaa gcgagcgctt cgcaaaaaaa gcaggagata tcggactttc tatcgggatg
     6661 gcctttcaga ttatcgatga tattttagat tatacaaaag agagccagga aatcggcaaa
     6721 cctgtactag aagacatgcg tcaaggggtc tattctttac cgttgatcta ctctttgcaa
     6781 aaaaacaaac caaagctact accttattta gagaagaaag cggcaatgac agaagaagat
     6841 gtcgacgccg tacgcaaaat cgttgaacat acaggaggcg tggaagaagc taggaagtta
     6901 gcggcctctt acacagaaaa ggctttgaag gaaataaaga aattacctgc cacctctctt
     6961 cgaacaaaag agaatctctt ttcccttacc caactgattt tggatcgcaa agactgaata
     7021 gggaaaggtt cgatacgtaa ataaatttct cagaaggaaa tctacgtgtt ttttgacaaa
     7081 ttgaattgta gttcttgtat ttatgataaa aagcaaaaat aaagagcaac gtccgttagc
     7141 gacgttgctc ttttttatct tgaatatttt tttacctttt cgatttctta taactcataa
     7201 aagtgattct taaaccgatt acaagtaata taacgcctgc gagtgttatc caattattcg
     7261 attgacttcc agttttagga agtgctcttt ttgatttgtt tccattgtca ttgcttgttt
     7321 tattagttaa gttaaagttc gtcctgttat ccgaacttgt ttttgagaag gagacacttg
     7381 catgacgtgt atcgcttgtg gtagacgacg tgtctgtact atcgcttgaa gttgatgaat
     7441 ctgagctatc acttgtggta gaggatgtat ctgtactgtc acttgaagct gacgtgtccg
     7501 tgctatcact tgtagtggta gatgaatctg agctatcact tgtggtagag gtagtatctg
     7561 tactgtcact tgaagctgac gtgtccgtgc tatcacttgt tgtggtagat gtatccgtac
     7621 tgtcgcttga agctgacgtg tccgtgctat cacttgtggt agaggtagta tctgtactgt
     7681 cacttgaagc tgatgt
//
LOCUS       DANJLF010000098         7669 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000098 DANJLF010000000
VERSION     DANJLF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(59..787)
                     /locus_tag="PWT25_002502"
     CDS             complement(59..787)
                     /locus_tag="PWT25_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1022535.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="PWT25_002503"
     CDS             complement(800..2080)
                     /locus_tag="PWT25_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1022536.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="PWT25_002504"
     CDS             complement(2221..2823)
                     /locus_tag="PWT25_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL1022537.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="PWT25_002505"
     CDS             3068..3274
                     /locus_tag="PWT25_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022538.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="PWT25_002506"
     CDS             3586..4305
                     /locus_tag="PWT25_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL1022539.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="PWT25_002507"
     CDS             complement(4386..5069)
                     /locus_tag="PWT25_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL1022540.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="PWT25_002508"
     CDS             5287..7473
                     /locus_tag="PWT25_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL1022541.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2745 a         1292 c         1442 g         2190 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagtttat tcggaaaagg gaggtgtgac
     7621 aagaattttg tcacacctcc cttatttatt ttgaaaatga gaatactat
//
LOCUS       DANJLF010000099         7038 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000099 DANJLF010000000
VERSION     DANJLF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..777
                     /locus_tag="PWT25_002509"
     CDS             <1..777
                     /locus_tag="PWT25_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1022542.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(782..1546)
                     /locus_tag="PWT25_002510"
                     /pseudo
     CDS             complement(782..1546)
                     /locus_tag="PWT25_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
     gene            1742..3487
                     /locus_tag="PWT25_002511"
     CDS             1742..3487
                     /locus_tag="PWT25_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL1022543.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(3767..4204)
                     /locus_tag="PWT25_002512"
     CDS             complement(3767..4204)
                     /locus_tag="PWT25_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL1022544.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(4188..5561)
                     /locus_tag="PWT25_002513"
     CDS             complement(4188..5561)
                     /locus_tag="PWT25_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022545.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(5883..6371)
                     /locus_tag="PWT25_002514"
     CDS             complement(5883..6371)
                     /locus_tag="PWT25_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1022546.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(6393..6995)
                     /locus_tag="PWT25_002515"
     CDS             complement(6393..6995)
                     /locus_tag="PWT25_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL1022547.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2271 a         1463 c         1241 g         2063 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca
      721 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
      781 ttaagaattt tgcttcataa tcatagaatg ctacgtcttt tacgacttca ccaggcaaag
      841 tcgtccgaac gtcttcattt cctaatacag caacttcgat ttcgcgcgct tcaattcctt
      901 gttcaacgat tgctcgagaa tcatactgat aggctgttgc taaagcattt tgcagctctt
      961 ctcggttttc tgcttttgta atgccgacac tagaacccat attcgccggt ttgacaaaca
     1021 tcggataaag caaagaacct tcacattgat caaatacttt tttaggattt tctttccatt
     1081 gattcttaag tactggtaca taaggaactt gcggcacacc agcagcttgt aaaatatact
     1141 tggtcatgat tttatccatg gcacatgcac tggtcaatac gcctgcgccg acataaggca
     1201 tattcaatgt ctctaagaag ccttggatcg ttccatcttc cccgtttggc ccatgtaaaa
     1261 ctggaaaaac gatggctcct tcttctttga tttcgcccgg attgatcact tttcctgtaa
     1321 agccttcctc tgtctgtcca cttgggtccc atgaaagatg caagacatct ttgctggcag
     1381 gtttttctgt tagtaatgga cctttgaccc attgtccttc ttttgtaata aatacgagtt
     1441 gaacttggta ataattataa taaatggcat ttaaaactga aaatgcggaa agaatggaca
     1501 cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga ttgtcctcct
     1561 gtattctaaa tcgttcagtg atttactctc atattttagc acaaaacaaa aataaacgaa
     1621 taacttattt ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt tattttttgt
     1681 cagctagatt atttgtcccg ttttttataa aaagttatga taaagaaaag gagggatgat
     1741 gatggcgcaa acaaatccac gcagccgctt ggcagaaatc attcgagtgt tcatcaatta
     1801 caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa aagcctttga
     1861 agaactaggc cccaccttca tcaagatagg acagatgctt tctgttcgag aagatttgct
     1921 ttcttctgcc ttcactcaaa cattcaaaac attacaagat agtgtaccta gcgacacatt
     1981 ttcaacggtc aaaaagacga tcgaaacaga actttctctt tcattatcag atatattcga
     2041 tgatttttca aaatcgcctt ttgcctctgc ttctatgggg caggctcatc gtgccaagct
     2101 gaagaacggt gacgcggtag tcgttaagat ccaacaccct aacatcgccg aagagatccg
     2161 acttgatctt caactatttg aacgagcgat ccctttgatc aaatacattc ctgaaactag
     2221 cgtagttgat ctaaaagggg tactccaaga agtaaaacgt tccttgatca atgaaatgga
     2281 cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa aagaacaatg gctggaaaga
     2341 gatccgcagc cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg tgatggaaga
     2401 aatgtctggt aaaaacttga atcatttaat gaacgccgaa aacaaaactg aaacttttat
     2461 cactggaatt caaaacaagc agctgaaaca agaagttgca aagcttttag ttgagaactt
     2521 tatgaaacag gtgtttgatg atggtttttt ccatgctgat cctcatccag ggaatctgct
     2581 attccatgta ctaacaaaag aagaacagac acaagctagc cgaaaaactg aaacagtcca
     2641 tgaaaaagag ttcggttcct ttgcttttcg agcctccact tctgcagaag accctgttgc
     2701 gccatatacc atcaactaca tcgattttgg tatgatgggg catctttctg caggacttag
     2761 gcaaaaactc acacaagccg ttttagcgct gtatacgaaa gatgcttatc gaatcgaaaa
     2821 agcggtcctt cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt tccaccaaga
     2881 actgacaagt tttcttgaac agtattatga ttctcctatt gatgaaatca acttgcaaga
     2941 agtattcaca catgtcgtga cgatttgtca tcaaaataat ctgcagttcg atcgtgatat
     3001 cacgcttttg ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc tcgatcctga
     3061 agtttccctc atggaagtcg cttcgccttt tgctcaacat tattttctca ctcatctgga
     3121 tgtagaagat acattgaaac aaagcggatt agacttattg gaagggatga aggctgcgcc
     3181 taagatccct caacagctcc accatctatt agaaatgtgg acttcgggac aaggaaaagt
     3241 caatttggaa ttgaaaaagc aagacaaatt attgagcagg atcgaaagta tgatcaatcg
     3301 gcttgttttc ggtatgattt tagctgcatt gatcgtaggg tcctccctgc ttgtccaagc
     3361 agcacctgta gaaaacgcag aggtcgtttc cttattaggg atatttacgt atgctattgc
     3421 cgcattcgtc attatttttt tggcaatcga tgcattgatc caaatgtata agaagaggaa
     3481 aaagtagaaa aaggttgtga cagaaagaat cagcttcaag aaataagaag gggttttaca
     3541 taaattgttt ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg ctacttctga
     3601 aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc cagtaataag aacaatcaaa
     3661 aagggaacat ggaacacatt tatcctgttt ccctgttccc tttttgatgt attcgagcat
     3721 aaataaaata ccttcgttac ctactatctt ctgtacttgg taaaccttag tcaattgcct
     3781 tttttaacgc ttgacgaaaa gacatatgat ctttctccgt aaagggtttt ggaccctttg
     3841 tccttatgcc agcttttctt aactttccac tatgataacg ttgttctaga agcgtgccga
     3901 tattcgcttc gtcaaattct tttccagagt gatggaacac tcgcacaccc ttcggcaata
     3961 caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc attcttctta accataccga
     4021 cgattttata atccgcacta tcagctccgc ggtctacgta gacgatttgg acatgattag
     4081 cgtattcttt ggtcgtataa tggtccacac tggtcacgat aaccacgtca agaccatagg
     4141 attctcctaa ttttatgaca tcttctttta ctggagaacc atctccatca atgaatagcc
     4201 tcattctttt tcgccttctt ccataaatga taaacagtta ctaatccgct cagtaaaaga
     4261 ccaacattga tccattttgc caagataagc agttgtacac tttctatcgg cactgattca
     4321 tggttcagcg tgatcaataa tagatggatt ggctctccca tttcttttgc taaaacaaac
     4381 aaaagcgtca tcaataaaaa ctggtggatc acactccctt tattttgagt agtatacttg
     4441 acaacgatcc ggcgatccat tggaatagct gtatcagttt gataatattc cttatttagc
     4501 cagctgcctg ttactacgtg ccagaaacta agcagaaaga cacctatcgc aaaaccattt
     4561 ggaatcaaga ggaccaataa agaagaactc agtccaagca attgcatagc caatggagac
     4621 catgttaaac gtttcaaaat ccatggtccg actatttttg ctgcaatcaa gcctgctgca
     4681 taaggcaaat acataaaaat cgaaagtttt tctaatccgt acagtatgcc aacataaaaa
     4741 ctgctaaata aaaaaaggaa attccctagc attccattta gaaaagtaaa cagatttagc
     4801 cagcttggca acttccattc cttcttctgg tgtcctaaaa caaacacgaa caagatgaac
     4861 agcagcaaga aaccataaat agcaagatca aaaagccttt catcgaataa caatcgggca
     4921 tttctcaaaa gaaacaataa gccaaagaat atcaaaaata aaacaagctc tcgcgtagct
     4981 accagatttc ttttgatgtc tttgaaatcc agttcgtaac gcggataatg actaaccgta
     5041 tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat ctgagtcggt
     5101 gcaggcaact caatcgcttg atacaggaga aatagccaga tagctgcaaa taagtacttt
     5161 ttcccatcca tattgataaa accttgcgtc ttttcgtgaa aattgaccgt ggtattagca
     5221 ggtacaagcc atgctgcact cagccctagc aaaacacttg aaaaataaaa taaaggaaaa
     5281 taaaatacgc ctaatattcc aagcaatgaa ccagcaccac caatcagcag agcgatcatc
     5341 agcaacgtgt aactatctag accaaatcgt agactgttga ctaaaaatag tccagtcatc
     5401 cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga gtctgttagt
     5461 cccaaaaata aaagataagg aataaaaata accgtgttac ttaagataaa gggcgttgtt
     5521 tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc tccttaaaca
     5581 gtcttataac taatctgctc ttcgatttta gcattaaagg ggaaataaac caattttaca
     5641 gaatatattc ttgtaaaaaa aaatacagaa agaagcataa gcttctttct gcatttttac
     5701 ggtacttgat tactcaagca gacaagccct tctccacaaa agtcttgccc gacacccctg
     5761 catccggcag gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat caattaggag
     5821 ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt tactttaaca cagttcctat
     5881 gtttatgcga gagctttttt cattttataa tcgaccattt gaaatccttg accgatatct
     5941 gaaataagtt cttctgtaat ctcaaatcct tcatgcttat aaacctcgat tgcttgatga
     6001 ttcccctgat tcactcgcaa aaagatctct ctttcctttg tttgcgcagc aagttcttca
     6061 taccagcgaa aaactgaaga agtcaatcct tgaccacgta ctttatttag caaatataat
     6121 ttacttaaat aaatttttcc atttatctct tcataagcag tgtaaccgac agtcttttct
     6181 tcgcttttta ataaaaagta ggacacacct tctgtcagtt ctttttgaat ctgttttttt
     6241 gactgataag ttttcagcat gtaatccact tgtttttgac caatgatcgg cacaaaaact
     6301 tccggccata cttcttgtat gactgccatc agctcatcca gttcattttc tgttactttt
     6361 attttttcca tacacccacg tcctttttta cattataaat gaccttgcgc ttgatggaat
     6421 aaaaagagca tgatcatgct atacacaccc accgaccacg gtttcaagaa aatcacaatc
     6481 tgcataaatt ccctaaaaga taatttcgtc aaaccagcta ctagacagat caagtcgtct
     6541 ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt taatatcttt cgtttttctt
     6601 gtcagcaatt catcaaattt ctgatacttt tttggcgata aaataagctg gacaaaggct
     6661 acgccatagt aacggacaag caaaaatccg attgcttccc caatcactag gccaatacaa
     6721 ctatataaaa gcccaaatcc attgccaaac atcagcatgc ccgctactga ggagatccct
     6781 cccggcaaaa taggaatgat cacttggatc acttgcaaca gaacaaatat catcactgca
     6841 tacttgccaa attgctggat aaacgcttgc aaagacgtgc tcgatcgaaa aatcccatga
     6901 tgataagcat aaagaatcaa cacaaagaaa aaaaacagac caataatggg catgatctga
     6961 agacattttt gtaaaactga tcctttttta gacatctata ttccccacat cttttttcat
     7021 ttattaaaac tgcctcct
//
LOCUS       DANJLF010000100         6962 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000100 DANJLF010000000
VERSION     DANJLF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            110..491
                     /locus_tag="PWT25_002516"
                     /pseudo
     CDS             110..491
                     /locus_tag="PWT25_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            622..1572
                     /locus_tag="PWT25_002517"
     CDS             622..1572
                     /locus_tag="PWT25_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL1022548.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2545
                     /locus_tag="PWT25_002518"
     CDS             2090..2545
                     /locus_tag="PWT25_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1022549.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2886..3488
                     /locus_tag="PWT25_002519"
     CDS             2886..3488
                     /locus_tag="PWT25_002519"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022550.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3582..3914
                     /locus_tag="PWT25_002520"
     CDS             3582..3914
                     /locus_tag="PWT25_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HDL1022551.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4065..5044)
                     /locus_tag="PWT25_002521"
                     /pseudo
     CDS             complement(4065..5044)
                     /locus_tag="PWT25_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5049..5816)
                     /locus_tag="PWT25_002522"
     CDS             complement(5049..5816)
                     /locus_tag="PWT25_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1022552.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6306..6731
                     /gene="lacA"
                     /locus_tag="PWT25_002523"
     CDS             6306..6731
                     /gene="lacA"
                     /locus_tag="PWT25_002523"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDL1022553.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6748..6930
                     /locus_tag="PWT25_002524"
                     /pseudo
     CDS             6748..6930
                     /locus_tag="PWT25_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
BASE COUNT         2381 a         1069 c         1257 g         2255 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa
     4261 ctaagtttat tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt
     4321 ccattttcag gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta
     4381 tagtcgggtt tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga
     4441 ttgaatctca tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt
     4501 ttagataatt ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt
     4561 tctgcagctc ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga
     4621 taggcaccaa tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac
     4681 ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg
     4741 taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa
     4801 tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa
     4861 tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt
     4921 tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt
     4981 taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag
     5041 tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga
     5101 gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag
     5161 aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac agcattgttt
     5221 agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga attatgggta
     5281 acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc
     5341 attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca
     5401 atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt
     5461 ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca
     5521 ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt
     5581 tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa
     5641 agcaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg
     5701 tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg
     5761 gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga
     5821 agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata
     5881 aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg
     5941 aaataagaac agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat
     6001 aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat
     6061 agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg
     6121 gtgttatcta ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt
     6181 gctttttgtt tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga
     6241 acattttgca aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg
     6301 tcaaaatggc tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga
     6361 aaaattttct tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt
     6421 ttgttgatgt taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta
     6481 ttgtaattga tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg
     6541 ttgcagcaga agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac
     6601 gaatgattac ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag
     6661 gtttcgtcaa tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca
     6721 aaatgtgtta agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc
     6781 acagatgaaa aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat
     6841 ttcgggacat atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa
     6901 gcagtagtaa gtggtcaagc agacttggga gggttctgtt gcgaagtttg aaaatcaaac
     6961 gc
//
LOCUS       DANJLF010000101         6639 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000101 DANJLF010000000
VERSION     DANJLF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            46..783
                     /locus_tag="PWT25_002525"
     CDS             46..783
                     /locus_tag="PWT25_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1022554.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            797..1612
                     /locus_tag="PWT25_002526"
     CDS             797..1612
                     /locus_tag="PWT25_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL1022555.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1625..2272
                     /locus_tag="PWT25_002527"
     CDS             1625..2272
                     /locus_tag="PWT25_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1022556.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2288..2944
                     /locus_tag="PWT25_002528"
     CDS             2288..2944
                     /locus_tag="PWT25_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1022557.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3106..3927
                     /gene="racE"
                     /locus_tag="PWT25_002529"
     CDS             3106..3927
                     /gene="racE"
                     /locus_tag="PWT25_002529"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL1022558.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3930..5285
                     /gene="rph"
                     /locus_tag="PWT25_002530"
     CDS             3930..5285
                     /gene="rph"
                     /locus_tag="PWT25_002530"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL1022559.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5286..5804
                     /locus_tag="PWT25_002531"
     CDS             5286..5804
                     /locus_tag="PWT25_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1022560.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5911..6411
                     /locus_tag="PWT25_002532"
     CDS             5911..6411
                     /locus_tag="PWT25_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1022561.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2175 a         1115 c         1465 g         1884 t
ORIGIN      
        1 atctttaaaa gaaatcttac taaataataa tgggagtgta attctatggc aatgattaaa
       61 tttgatcacg tggataaata ttatggcaag ttccacgcat taaaaaatat caatctcgaa
      121 ttcgagaaag gagaagttgt cgttgtcatt ggtccttctg gttcaggaaa gagcacgatg
      181 cttcgctgta tcaatggatt agagacgatc tcttcaggta agctgctgat taatgatact
      241 gacttgcatg ataaaaagac aaaactgaca gaagtccgaa aaaacatcgg catggttttc
      301 caacatttca atttgtaccc aaataaaact gttctggaaa acatcaaatt agcaccaatc
      361 aaagtgttga agcaagacca ggcaactgca gtcaaaaacg cggagaaatt tttgaaaact
      421 gtcaatatgt tagataagaa agattcttat ccttccatgt tatccggggg acaacagcaa
      481 cgggtggcaa tcgccagagg tttagcgatg aatcctgaga tgctgctgtt tgatgaacct
      541 acttctgctt tggatccgga aatgattggt gatgtattag acgttatgaa aaaattggct
      601 catgacggta tgtcaatgat tgtcgtgaca catgaaatgg gctttgccaa agaagtggcg
      661 gatcgtgtta tcttcatggc tgatggagaa gtattggaag acagcagaaa tgtgagagac
      721 tttttcgaaa atccaaatga agtccgagca cagcaattta tcagtaaagt tatcaatcat
      781 tgataggagg aaaaagatgc gcaggttaaa aaaaatacgc ctgattttcc ttttaggcat
      841 tgcttttctt gcactagccg gatgtaaagg aaatgggttg gcaaatgaag atattttaga
      901 gcgaagcaaa tctaccaatg aaatcatctg gggtgtcaag tatgatactc gattatttgg
      961 aatgatggat attgagagca gaactgtaca aggatttgat gtagatattg caaaagcaat
     1021 cactaaaaag atccttggtg acaatggaaa aacagaattt gttgaagtca cttctaaaac
     1081 tcggattcca ttattgaaaa acggaaatat cgatgcaatt attgcaacga tgacgatcac
     1141 agatgaacga aaaaaacagg ttgacttttc agatgtctat ttcgatgctg gccaagcttt
     1201 gttggtaaag aaaggaagcc aaatcaaaag tgtagatgat ttgaatgctt ctacaacggt
     1261 tctagcagtc aaaggttcta catcggctgc gaatatccga cagcatgcac cagatgcaaa
     1321 aattttagaa ttagaaaact atgcagaagc atttactgct ttacaatctg gtcagggaga
     1381 cgcgatgact acggataatg cgattttgtt agggattgca gatgaaaatc cagagtatga
     1441 gttagttggt ggaacgttta ctaatgagcc ttacgggatt gcgatcaaca aagggcaaga
     1501 aaacttcttg aaagctgtta accaagcgct agaggaaatg catgcagatg gcacatacga
     1561 taaaatctat caaaaatggt ttccaaatga aactgaagga aaagtagagt aagggggaga
     1621 ggcaatgagc gaattgatac aaacttatgg accaatcttt gtcgacgggt tcaagatcac
     1681 attgttttcg agtgtcttag cacttgtgtt tagtttgatt atcggaacgt taatggcaat
     1741 cttccaatta tcaaagaata aagtagtgag cgccttagca aaggcatatg tagaattttt
     1801 tagaaacatt ccattattaa tcatcgtgat gtttttttat gttgtcgtac cgatttattg
     1861 gtttaatcta gatggattcc aagcaggtac gattggatta acgatctata catctgcttt
     1921 tattgctgaa acagtacgtt cagggattca aaccgtacca aaaggtcaaa cagaagcagg
     1981 actatcttct ggttttacct attcagaaat catgcgttat attgttttac cgcaagcatt
     2041 caaaatcgtt atcccgccat taggcaacca atttatcaac ctcgtaaaaa actcatctat
     2101 tttagcaatc gtagctggtt tagatttgat gtatcaagga gacttgatcg ctagtgaaac
     2161 attcaatacc ttcgatacgt atatcatcgt tgggttattt tacttagtta tcacgctacc
     2221 gttatcttac ttaatggttt atcttgagaa aaaatgggcg gttcgttcat agaaggaggg
     2281 gaaatacatg gattttagcg gcgctttttc atggatgaat atccgttttt tattggaagg
     2341 attaaaggtt actgttgaag tttcattgtt ttcgatcatt tttagtttct tgatcggtgg
     2401 tctgacaggt gtgattcgtt ttgcgaatat ccctttcttt tccaaaatcc ttggtgtagt
     2461 gattgatatt atacggaatc ttccattatt attgatcatt ttcttcactt actttgcctt
     2521 acctcaaatc ggcattcaga tgaacatctt ctggtccgct gtcgcagcat taacgatttt
     2581 tgagtcagct atgttgtcgg aaattttccg agctggactc aatgctgttc caaaaggaca
     2641 gatggaagca ggattatcaa cgggactgac ttatgtagaa tcgatgcgaa cgatcgtgtt
     2701 accgcaagca tttaaatcaa tgatcccggc aattgtgagc caattgattt cactgatcaa
     2761 agatacttca ttagcagtaa tcatttcttt acctgaactt tctcatcagg cacgtattat
     2821 ttatggacag aacaccaatt atgtgcttcc gatgttcatc atcatgacac ttatgtactt
     2881 cgtggtatgt tacgcgcttt ctctgctttc cggttacttg gaaaagagac ggtacagtta
     2941 ttaatagatg attcttttag cggttgtggc aaactaaagt cacaaccgct tttcctcttt
     3001 ccattcgaaa aaaagagatt tcgattgttt taagcaagta agagcacttg aaaattcacg
     3061 ctaaaaagta cacaataggg aggatagaat ttattgaaaa aaaggatgat acgattgaca
     3121 gataatcgcc ctatcggatt tattgattca ggtgtcggcg gcttgactgt agtaaaagaa
     3181 gccctgaaac aattaccgaa tgaaaatatt ttatttgtag gagacacagc acgctgccca
     3241 tatggcccta gacccgcgga acaggtaatc cagtatactt gggaaatgac ggattatctg
     3301 gtggagcaag gaatcaagat gctggtgatc gcctgcaata ccgcaactgc ggtggcttta
     3361 gaagaaatca aagctgctct ttctattcca gtcatcggtg tgatccttcc cggtactaga
     3421 gcggcagtaa aaaaaacaca aaataaacaa gttggcatta tcggtacgat tggtacggta
     3481 aaaagtcaag cttatgaaaa agcactgaaa gagaaagtac cagaattgac tgtgacaagt
     3541 cttgcttgtc ccaaatttgt ttcagttgtc gaaagtaatg aataccattc atcggtggcg
     3601 aaaaaaattg tggcagaaac attagctcct ttaaccacta aaaaaatcga tacattgatt
     3661 ttgggatgca cccattatcc attattacgc cccatcattc aaaatgtaat gggagaaaat
     3721 gttcaactga tcgattctgg agcagaaaca gtaggtgaag tatctatgct gttagattat
     3781 ttcaatttga gcaattcacc gcaaaatggt cggacattat gccagtttta tacaactggc
     3841 tctgccaaac ttttcgagga aatagctgaa gactggcttg gaatcggaca cttaaatgta
     3901 gaacatatcg aattgggagg aaaataagga tgcgtcatga cgggagaaaa gcccaagagc
     3961 ttcgcaaact ggagcttcaa acaaatgttt tcaaatatcc agaaggatcg gtagtgatcc
     4021 gttttggtga tacaacagtt atttgttctg caacaataga aaattctgtt cccccttttt
     4081 tgagagaaac aggaacagga tgggtgacag cagaatatag tatgctgcct cgagcaacga
     4141 gtacgagaaa tcgtagagaa agcagcaaag ggaaactttc tggtagaaca atggaaatcc
     4201 agcgtcttat tggtcgttcc ttgcgtgcag tagtagactt ggaaaaatta ggagaacgta
     4261 gtatcatcgt agactgcgat gtgatccaag cggatggcgg tactagaaca gcaagtatca
     4321 ctggggcatt tgtggcattg aaattagcca ttgaaaaact tttaagagaa aaagaattgt
     4381 cagaagatcc aataaaagaa catcttgctg cagttagcgt aggaatctta ccagatggga
     4441 cttgcgtcac agatttggat taccaagaag attccgcagc acttgtagac atgaatcttg
     4501 taatgacaga atctggaaaa ttcgtggaga ttcaaggaac tggagaagaa gcaacgtttg
     4561 atggtgaaca gttgaatgaa atgctctttt ttggaaaaaa cgcgattgaa gatttgatca
     4621 aagagcaaaa acatgctttg ctaacagagt ttgctcaaaa tgatgagcgg atagaagaaa
     4681 caaaaacaat catcattgcg acaagaaatc caggaaaagc agaagagttt cgaaatatgt
     4741 tcaaagaagc gggatatcat gtaaaaacat tacttgacta tcctgaattg ccagatgtgg
     4801 aagaaactgg cagcacattt gaagaaaatg cccgattaaa agcagaaacg attgcacaat
     4861 tgcttgatca gccagtctta gcagatgatt caggtttgaa agtggatgct ttaggcggta
     4921 tgccaggaat ctattctgca cgttttgcag gggaacaaaa aagtgatgcg gggaataacg
     4981 ccaaattatt atatgaattg acagatgtgc cggatgaaaa aagaacagca caattccatt
     5041 gtacgttagt attcgcggca cccaaaaaag acagccttgt agtagaagcg gaatggcccg
     5101 gtcgagtagc ccgtatacca tctggcgaaa atggctttgg gtatgatcct ttatttatcc
     5161 cagaaggtaa gaaacaaaca gcagcagaat tatcgagcga agaaaaaaac aagatcagtc
     5221 atcgggctca agcaatgaaa aaattgagtg ccgagtggaa acaatggctg gaaggagaga
     5281 gatagatgcg gtatttagta gtaagtgata atcatggaga cagagatatc ctcgttgact
     5341 tagtagatcg ttatcgttct gaagtggata tctttttcca ttgtggtgat tcggaattag
     5401 aagcttctga cccattatgg gatacattcc aagtggttca aggtaattgt gattatggac
     5461 caggattcga acagaaaaaa gtgatccaaa ctggtcagga tacagtattc atgactcacg
     5521 gtcatttatc gaatgtccga tttggtttga cacagttagc aatcgaagct cgagctgcaa
     5581 ctgccgatat ggtgtttttt ggtcataccc atcaaatagg ctgtgagatg gatcagaaaa
     5641 tcctgttttt gaatcctggt agcatttctc agcctagagg cccgatccaa atcccatcat
     5701 atgctcttat cgaaagttta gatgaccaaa tttctgttca atactataac cgtgcccatc
     5761 aggcagttga agagttggcc ttttcttttt cgagagagcg ctgaataaaa aaactctaat
     5821 ttttaataaa aataagcttt attttatgaa ttttgcatcc ggtttcggta aaatagtagt
     5881 gttgaatgaa atagaaatgg aggccatcag atgattggac ctatagtaag agaaatgctg
     5941 ctggaaaatc aggaaacgtt tttgattcct gcagaaaatg tggcaaatgt tatgtatcat
     6001 catccattgt cccatggttt acttgtgtta tcgaaggtcg gttattccaa aattcctgta
     6061 ttaggaaaag atgatcactt tgtaggacta gtaagtttat cagatgtggt caataaaatg
     6121 attgacttgc aaacaatcag catggacccg ttagaaggac taacggttgc tgatgtgaca
     6181 gaaacagatg ttccgacaat tgatgaaaat tgggaactag aagaagtact ccatttgtta
     6241 gtcgattcgc cttttttacc ggttgtcaca gaagatcgga ttttcaaagg aatcattaca
     6301 agaaaagagc tgcttaaagc cgtaaacttc atggtgcatg aattagaacg tcgtaatata
     6361 ttgtctccga aaatcgaaga attaaaagaa caaatcgata tcgtgagcta aagaacgaga
     6421 aactaaaaga taatcaaaaa ataagtagaa aaaacagcgt accagttttg actttatctt
     6481 gaaaaagagg ctgggacaaa agcgtttaac tccaagaaat aagaaggaat tcacgaaaat
     6541 tgcttttcaa atttttgtga atttcagctt atttccgaag gagttgcttc tgcttccgcc
     6601 gtttatacgt gtttagagcg tgggacaaaa gtgttcttt
//
LOCUS       DANJLF010000102         6624 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000102 DANJLF010000000
VERSION     DANJLF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            140..2815
                     /locus_tag="PWT25_002533"
     CDS             140..2815
                     /locus_tag="PWT25_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDL1022562.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2997..3236
                     /locus_tag="PWT25_002534"
     CDS             2997..3236
                     /locus_tag="PWT25_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022563.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3252..4625
                     /locus_tag="PWT25_002535"
     CDS             3252..4625
                     /locus_tag="PWT25_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1022564.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4629..6263
                     /locus_tag="PWT25_002536"
     CDS             4629..6263
                     /locus_tag="PWT25_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1022565.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6426..>6624
                     /locus_tag="PWT25_002537"
     CDS             6426..>6624
                     /locus_tag="PWT25_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022566.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2322 a         1071 c         1329 g         1902 t
ORIGIN      
        1 tccaaaatca caactttttt cttttgtcaa gattttgagt gggatatatt tttgtagaac
       61 tgaagatcat gaaattatga gaggttggag gtaatgtagt catacaaata aaaatttttt
      121 agaaagtgag tggtaattaa tgaaaatgaa tcacacttca attcagaagc atttacaact
      181 gttaaataca ctggggattt cttatgttaa tttgagaact ttttctacat taaacaagaa
      241 aaccgtttct catatttttt ctactgcaga ttctcaagca attattaact atttagaaaa
      301 acagaaattg tctgaaggta cagtaaacat aacttataat cctattaaac aagaggtgat
      361 tacacaaaaa aagcattctg tgagagacaa tgatattgaa aaaattcggt ttgtatttat
      421 tgatattgac cctaaaagga aagagggaag tgcaacagat agcgaaaaga aaaaggcaga
      481 aaatgttatg gaacaagtag aacgttattt gaaagaaaag agaatagaat catttgtaaa
      541 agttgatagt gggaatggct accatatttt tcttcccata aatgaacaac caaataatca
      601 cgagacgatt cttactattc agaattttct tcaattactt caccgaagat ttggagtaga
      661 agatgaagtt gatattgata cttctgttta taatccttca agattatgca aattagttgg
      721 tacaccagcg gtcaaaggaa agtccacaaa agagcgtccg catcgtttat ctagtttcct
      781 ttctattccc gataacatag atagaagtaa ttgttttaaa attcttgaac agatagttga
      841 agaaaattcc atgatgtcaa cctctagagt aaaaagccat ccatctttta gagaagtgtt
      901 accttttatt caagcagact ataaacaatg gttagctcac tatcctgaat taacttatac
      961 cataaaagag ggcgatcata aaggagttac cttacttata tttgacgaat gtccattaag
     1021 aaaacacacg aataattcta atggttcttg tttgtctgtt acggctgata ataagattcg
     1081 atttcattgt ctgcatgctt cggataaaaa taaagatatt caagactttg taaaaaaata
     1141 tccgttgcca aaacctgtaa atccttctgt agaatcatta gaatccaatc aagaatatta
     1201 ttttgaccag tttaaactga atcaagaagg agtatttttg gtgaaagaag gtcaagaaga
     1261 aaaagttgct tgtccgatgt ttattaagca aatacgcaaa aatatcgaac aaaaagtcat
     1321 tacacttgtt cttgcctatt atgtcgatca ttgttgggat gaaatagaaa tagaaggtgg
     1381 atgtttacaa actaataatt ttaaacaatt agcaaaacat ggcttaatca ttaaagctag
     1441 aagagaagct gaagtcgttg actttttaca gattcaacgg gagaatgctc caatcttttt
     1501 tgaacatgaa tctcttggct ggaaaaaaga aaatgaagca atgatttttc agttacaaga
     1561 ttcttttgga cgtgaaattc cttctgtatt aactaaaaac tcatgctacc aattaacaac
     1621 taatggtaca gaagcggaat ttgacgcatt ggttcataag tatgttttag gaacgaactt
     1681 agaacttgct tggtcgattg gtattgcttc tattgtgtta ggctatttaa acgttacgaa
     1741 ggctaatact tttttgagtt tactaattaa cctacagggc aagagcacaa cgggtaaaac
     1801 cactacgtta cactttattg cctctttatt tggaaatcca actacggttc ttagaaatat
     1861 gaatgctacc aataaagcca ttataaagct agcatcaaac aatcagggag gtgttccact
     1921 aatactagat gagttaggag cagcaactca aaataacttt agctcgttga tctatcaatt
     1981 agcttcagga gaagaaaggc taagattaga ttctaattgt cagcttatgc cacaagagac
     2041 tttttctgtt ttgaccctta cctcttctga agagcgtttg gaaaattatt tcaatgagaa
     2101 attaactggt ttgaaagttc gatatatcga attttctgat atcaagttta caaaggatgc
     2161 gaatcacgca gagaaagtta aaagtctttc ttctaatact tttggttggg gtataaagtt
     2221 atttcttttg aaactctttg aacaagggat tacaaaaatt gaagcaactt ttgtcgaagc
     2281 tcaaaaaaaa ctatgggctg ttttacctga acattctttg aagcaacgca ttggtaataa
     2341 tctagctttg atttatagtg gagccctcct aattcaagat atactgggct atcctttacg
     2401 attagagcat atacagacgg tgcttattaa ttcgtacgaa cagttaattg tagatactag
     2461 tttaggaagt gagcaaacgg tatttgaccg agtgaaaaca ttgttggtga tgaatagcca
     2521 taaattttta acaaacagtg acaacaaaca tacgcccagt acgatttggg gaaaagtatc
     2581 aattcaaaaa aatggggata ttaaggtaaa tatttttcct atcccgtttg aggacttgtt
     2641 aaggcgagaa tttcaagtaa aggatctaac ctatcttttt aggcaattgc ttgattcggg
     2701 taatatgtgt gcagaaaaag ggagaagaac gaaacgtatt cacatgaatc gtcaagttct
     2761 tccaacatac gagattctat taccttcttc actcaaatcg tattttcgat tgtaatttag
     2821 aataaaccaa agtatgaaag tgaggcacca tgttatggaa atatttctta ttgatttagc
     2881 aaaacaactg attacttatc tgtatgatag cgagcaaacc caaacggaag taaatgaagc
     2941 ttgatctatg cttcatttac ttcattcaat caaacatgaa agaaaggaaa attctaatga
     3001 ctaaaaataa aatgacacta aaagcagagg ttcttctgta tattcaagaa catttttcaa
     3061 atcaagcctt ttttacaaaa ccaatttact tggatttcga aataagaggg gtatctgctg
     3121 gatctatcgg gggtactctt caagcgttga agaacgaagg atatttagaa aatcactttg
     3181 tgcaacgttc gttcaataga agagtcgtga aaaaatggta tttagttcat tcatagagag
     3241 gggaatcaag catgcgtgtg gcttgttatg atcgaacgtc tgtttatgaa gaagcgaaaa
     3301 ttagtaatga cgctcaatat gatgcagtga aagagtatgt tttagagaaa tatgaacaaa
     3361 ttccccaacg attttcagac aaaggagaat caggggaaaa agcggagcgt ccagcttttc
     3421 gacagcttag agcttgggtt tgtgcgtcag aagaagaggg ggtgatctat gtttggcgtt
     3481 atgatcgatt ggcacgtcaa gccaaaatag ccttagaatt tattgagttg tgccaacaac
     3541 atcaggttca agtgtattcg atttctgaac ctcttcctgc ttctttctca cacgcgtcaa
     3601 cgaataaact atttgtgcaa ttattgttca cttttgcaga acaacaacga aacgtcatta
     3661 ttgagaatgt tcgtgccgga ctttcctata aaaagcaaca acggcaatat ttgtcttctg
     3721 cggtaccata tgggtatcgt ttagtgaatg ggaaaatcca acaagaagta cacgaagcaa
     3781 ggattgttcg ctatattttt caactttatt tgaccaaaaa atatggctac aaaaagcttt
     3841 gtcaacgatt gacgcaacaa aagcttttct tccgcgaacg tccttttcag ccctatcata
     3901 tttatagtat cctaaaaaat ccattgtact atggcgaaat caaaggagga tctttgggga
     3961 agtatctagg aacctttgaa ccgattctct cgaaagcaac ctttttacaa gcccaagaga
     4021 ttcgccaatc aaggtgtaca gctaaaaaag atacgtatct gtacttatta cgtcaaaaaa
     4081 ttcggtgtcc cttctgtggt cgtcatctga gttcgaagta tcaatggaat acaaagaaga
     4141 cgaagacgct tcattattat cattgtaccg accgaagctg ccaaggaatt tttcttcggg
     4201 cagaaagggt tgagtcagaa gtattggaaa agatacaggc tttttttcaa tacgaaggcg
     4261 tctatcaagc cttgattgcc caattaacgc aacaattagc cgaaaagcga aaagatgaaa
     4321 aacgcaaagt aatccaacaa aagaaacaag aagcacaatg gattaaggca tttgaagagg
     4381 gcaattgttc actggaagaa ctcaagcaac aactgcaagc tcttcaaaag ccgatgtcta
     4441 cttcttcgtt tctttcactc caacagtgtg aagcaaaagt gaaagaatgg cttcaactaa
     4501 aagacctctc tagtcgacaa ttattatgtg actatgtttg tcggattgag gtaacaaagg
     4561 ataaaacagt ttgtgggatt tacttccgac aactgacaca aaatatttta gaaaggaacg
     4621 aatgatctat ggaaatgata caaaccaaac gagtcgcatt gtatattcgc gtgagtacac
     4681 aagaacaagc tttagaagga catagtattc aagcacaaga acaaaatgga cgacagtttg
     4741 ccgaacggat gggctatgaa gtggtagaag tttatgccga tgaaggaatc agtgggaaat
     4801 ccacaaaaaa tcggttcgcc tatcaacgaa tgatgaacga tgctcggaac catcgttttg
     4861 atctactagt gatttggaaa ttgacccgtc tcggacgcaa tatgttagat gtcttacaaa
     4921 cagtcgagga attattgcga tatgacgtag gattacattc gatctcggaa caatttgacg
     4981 tgaccacttc gacaggtaaa ttgatgttac aactccttgg gagtttcggt gaatttgaac
     5041 gcaaccaaat tgcggagaat gttcaaatgt caatgcaaag cttagtgaaa gataaaaagc
     5101 gttatgcagg aggccgacgt ttaggctatg tcagtggtat tgacacgaat ggtcaaaaac
     5161 aattggtcat tgaaccagaa gaggcgaaaa ttgttcgtct gatctatgca aaatatttag
     5221 gcggagcagg gtatcgagcg attgccaatt ttttgaatcg tcaaggatat caaacagtca
     5281 aaaagaatgc tttttctacc acggcagtca aagatatctt acgcaataaa atttatgcag
     5341 ggtatttaga atatgcgcgc tacatcaatt gggaaaccaa acgacggaag gggaagaatc
     5401 cgagaccgat tttagtgaaa ggaacccatg aaccgattat ttcggaagaa gattatcaaa
     5461 aagtacaaga acgccttgct ttagaaggaa aacaacccaa atggaaccat acaggtgaaa
     5521 atgtattgac aggcttgtta cgttgtccag aatgtggcgc accgatggca gcgagtaatg
     5581 tcacaaatac cctaaaggat ggcacaaaga aacggattcg atattattct tgttcagtct
     5641 ttcggaataa aggagcaagt gtgtgtcatg ccaattcgat tcgtgccgaa caagcggaaa
     5701 catttgtcgc cgaccgatta aaggagatcg ttcagctacc agaagttctt ccccgattgg
     5761 tggcagcgtt aaatgaagaa atagtgcgac aaagccaacc attggaacaa gaactcgtgg
     5821 tgttactgga acgaaaggaa gaattgaaga ctaaaattga aaaatgggaa gctgctttgg
     5881 aagatagtcc tgaattgttt ccgatgttaa aagaccgctt agacgaattg actgaaaaac
     5941 gacgacagtt gcacattcga gaaaatgaaa tcttagggat ctttcaacaa ggagagccga
     6001 ttcaagtcaa agatgtccaa cggatattga ctaatttgga tcgttttctt gcccagagtg
     6061 agaagaagca aatcaaagcc ttgtatcgta cctttataga gaaaataacc tttgatccta
     6121 accataagga gatgttggaa attacgatga aattcacgca tgccgtggta caacaattga
     6181 atgagcaata tcaaatagcc gtatccaaaa caaaggatac ggctattttt gtgttaaaaa
     6241 caccctttac gttaacgatc tagtctaggt tatatcaaac aacgttagag gggcgctaac
     6301 gcttctctag cgatttgaaa tcgttagaga agtagttagg gaggtcctta tcataaatag
     6361 ggatatttat taatgaatat ttgaaatata attgtatata agaattaaaa agagtgggga
     6421 tagatatgaa aaagatgggt aagtgtattt tatgtgaaaa atacactgaa cttactaaag
     6481 aacatgtacc acctaaaaaa tcagggaata ctggaggtaa aaagaccaga actggtaatt
     6541 tagatgattt ccttaagagc gattttacaa aaggtgattt tccgaagggg attaaaagga
     6601 aacctcaagg aaatgtttat tata
//
LOCUS       DANJLF010000103         6610 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000103 DANJLF010000000
VERSION     DANJLF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(19..477)
                     /locus_tag="PWT25_002538"
     CDS             complement(19..477)
                     /locus_tag="PWT25_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1022567.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(490..1821)
                     /locus_tag="PWT25_002539"
     CDS             complement(490..1821)
                     /locus_tag="PWT25_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL1022568.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1832..3274)
                     /locus_tag="PWT25_002540"
     CDS             complement(1832..3274)
                     /locus_tag="PWT25_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1022569.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3261..4025)
                     /locus_tag="PWT25_002541"
     CDS             complement(3261..4025)
                     /locus_tag="PWT25_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL1022570.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4129..5004)
                     /locus_tag="PWT25_002542"
     CDS             complement(4129..5004)
                     /locus_tag="PWT25_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1022571.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5259..5343)
                     /locus_tag="PWT25_002543"
     tRNA            complement(5259..5343)
                     /locus_tag="PWT25_002543"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5307..5309),aa:Leu,seq:caa)
     gene            complement(5361..5432)
                     /locus_tag="PWT25_002544"
     tRNA            complement(5361..5432)
                     /locus_tag="PWT25_002544"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5398..5400),aa:Gln,seq:ttg)
     gene            complement(5442..5514)
                     /locus_tag="PWT25_002545"
     tRNA            complement(5442..5514)
                     /locus_tag="PWT25_002545"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5479..5481),aa:His,seq:gtg)
     gene            complement(5576..5660)
                     /locus_tag="PWT25_002546"
     tRNA            complement(5576..5660)
                     /locus_tag="PWT25_002546"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5624..5626),aa:Leu,seq:caa)
     gene            complement(5681..5751)
                     /locus_tag="PWT25_002547"
     tRNA            complement(5681..5751)
                     /locus_tag="PWT25_002547"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5717..5719),aa:Cys,seq:gca)
     gene            complement(5797..5868)
                     /locus_tag="PWT25_002548"
     tRNA            complement(5797..5868)
                     /locus_tag="PWT25_002548"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5834..5836),aa:Gln,seq:ttg)
     gene            complement(5880..5952)
                     /locus_tag="PWT25_002549"
     tRNA            complement(5880..5952)
                     /locus_tag="PWT25_002549"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5917..5919),aa:His,seq:gtg)
     gene            complement(5955..6028)
                     /locus_tag="PWT25_002550"
     tRNA            complement(5955..6028)
                     /locus_tag="PWT25_002550"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5994..5996),aa:Trp,seq:cca)
     gene            complement(6035..6115)
                     /locus_tag="PWT25_002551"
     tRNA            complement(6035..6115)
                     /locus_tag="PWT25_002551"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6079..6081),aa:Tyr,seq:gta)
     gene            complement(6117..6192)
                     /locus_tag="PWT25_002552"
     tRNA            complement(6117..6192)
                     /locus_tag="PWT25_002552"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6157..6159),aa:Phe,seq:gaa)
     gene            complement(6228..6300)
                     /locus_tag="PWT25_002553"
     tRNA            complement(6228..6300)
                     /locus_tag="PWT25_002553"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6265..6267),aa:Asp,seq:gtc)
     gene            complement(6310..6382)
                     /locus_tag="PWT25_002554"
     tRNA            complement(6310..6382)
                     /locus_tag="PWT25_002554"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6347..6349),aa:Val,seq:tac)
     gene            complement(6398..6469)
                     /locus_tag="PWT25_002555"
     tRNA            complement(6398..6469)
                     /locus_tag="PWT25_002555"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6434..6436),aa:Glu,seq:ttc)
     gene            complement(6484..6573)
                     /locus_tag="PWT25_002556"
     tRNA            complement(6484..6573)
                     /locus_tag="PWT25_002556"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6537..6539),aa:Ser,seq:gga)
BASE COUNT         1980 a         1461 c         1083 g         2086 t
ORIGIN      
        1 aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt
       61 ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt
      121 ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc
      181 ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc
      241 catatggatc agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg
      301 aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat
      361 agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg
      421 aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt
      481 ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag
      541 tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc
      601 tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc
      661 ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc
      721 agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc
      781 cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc
      841 tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat
      901 accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat
      961 attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata
     1021 ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg
     1081 aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt
     1141 tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga
     1201 tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata
     1261 accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat
     1321 ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata
     1381 attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc
     1441 tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa
     1501 tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat
     1561 ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat
     1621 gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat
     1681 atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt
     1741 gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat
     1801 aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg
     1861 ccataacata agtcacttct aagccgatga taatttgata atttttttca gttggtttaa
     1921 tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta
     1981 cctctacccg taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa
     2041 acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac cctaactctg
     2101 tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa
     2161 tgtttctctt attgatcaaa taataaaaag taacgtaata caaggcaaaa aagaaaattc
     2221 cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa
     2281 tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta
     2341 aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt
     2401 caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt
     2461 tgaccatctc tttattttct ttataagctg ctttatagat agctagacat atagctggta
     2521 taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt
     2581 tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta
     2641 aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat
     2701 tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat
     2761 tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt
     2821 gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg
     2881 gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc
     2941 caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag
     3001 caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga
     3061 ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac
     3121 caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg
     3181 gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat
     3241 tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca
     3301 tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga
     3361 tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact
     3421 tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc
     3481 gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa
     3541 taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat
     3601 ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga
     3661 ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat
     3721 tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg
     3781 aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat
     3841 gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg
     3901 aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc
     3961 ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt
     4021 cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca
     4081 tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct
     4141 tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg
     4201 ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc
     4261 agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag
     4321 gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga
     4381 ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt
     4441 taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc
     4501 atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat
     4561 atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct
     4621 atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt
     4681 tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc
     4741 cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga
     4801 atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga
     4861 aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc
     4921 attatgattc agctttttca tcacatacac cgttaattca cctcctctat atgataaaac
     4981 aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa
     5041 aaacgaccta agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca
     5101 taatttacaa aaatagttta acacataata aaagcgttat caatatcttt tttactcgtt
     5161 ttatgataat acttgtatcg aattgatttg gaggtaatta cataaaaaca aaaaaaatat
     5221 agcaactctc tataagaaag ttgctatatt gataattata ccggcggccg gggtcgaacc
     5281 ggcacgtcct caacggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
     5341 ggcaaattta aaaataataa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
     5401 agtcccttgc cttaccgctt ggctataccc caagaataaa gggcgaatga tgggaatcga
     5461 acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatcat
     5521 aaaagatgtt atttaatttc tcaataaaaa actaagatga agacatctta gtcaataccg
     5581 gcggccgggg tcgaaccggc acgccctcaa cgggcactgg attttgagtc cagcgcgtct
     5641 gccaattccg ccacgccggc aagttgattt attagtacta aggcggtaac cggatttgaa
     5701 ccggtgatga aggttttgca gacctctgcc ttaccacttg gctataccgc cattatacta
     5761 ttatcaatct ataaaataaa gaataaatta tagaaactgg ggtagctgga ttcgaaccaa
     5821 cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaaaagg
     5881 gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt
     5941 cgccacattc gccatggcag gggcagcagg aattgaaccc acactaacgg ttttggagac
     6001 cgctgttcta cctttaaact atgcccctat aaaatggagg agagtggatt cgaaccactg
     6061 aaccctaagg aacggattta cagtccgtcg cgtttagcca cttcgctact cctccatggt
     6121 ggcgcgggac agaatcgaac tgccgacaca cggagcttca atccgttgct ctaccaactg
     6181 agctaccgcg ccatatttta aaattattaa ttgaataaca agaacgacgg ttccgacggg
     6241 aatcgaaccc gcgatctcct gcgtgacagg caggcatgtt aacccctaca ccacggaacc
     6301 agttcattat ggaggttaac gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct
     6361 ctcccagctg agctaaacct ccaacattca attaaaatga cccgtacggg attcgaaccc
     6421 gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caacgggcca ggtaaatttg
     6481 ctacggagag taagggattc gaacccttga gacagtttgg caccatctac atgatttcca
     6541 atcatgctcc ttcggcctct cggacaactc tccagagtga aagaagctca atactctttc
     6601 tcaaaatgag
//
LOCUS       DANJLF010000104         6564 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000104 DANJLF010000000
VERSION     DANJLF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(7..1164)
                     /locus_tag="PWT25_002557"
     CDS             complement(7..1164)
                     /locus_tag="PWT25_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022572.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="PWT25_002558"
     CDS             complement(1167..1523)
                     /locus_tag="PWT25_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022573.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="PWT25_002559"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="PWT25_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL1022574.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="PWT25_002560"
     CDS             complement(2991..3830)
                     /locus_tag="PWT25_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL1022575.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="PWT25_002561"
     CDS             4015..4902
                     /locus_tag="PWT25_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL1022576.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="PWT25_002562"
     CDS             complement(5486..6181)
                     /locus_tag="PWT25_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDL1022577.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="PWT25_002563"
     CDS             complement(6165..6563)
                     /locus_tag="PWT25_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL1022578.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2065 a         1300 c          966 g         2233 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catc
//
LOCUS       DANJLF010000105         6462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000105 DANJLF010000000
VERSION     DANJLF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(114..704)
                     /locus_tag="PWT25_002564"
     CDS             complement(114..704)
                     /locus_tag="PWT25_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL1022579.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            1188..1415
                     /locus_tag="PWT25_002565"
     CDS             1188..1415
                     /locus_tag="PWT25_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL1022580.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1431..2597
                     /locus_tag="PWT25_002566"
     CDS             1431..2597
                     /locus_tag="PWT25_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL1022581.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2831..3874
                     /gene="hrcA"
                     /locus_tag="PWT25_002567"
     CDS             2831..3874
                     /gene="hrcA"
                     /locus_tag="PWT25_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL1022582.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3968..4531
                     /gene="grpE"
                     /locus_tag="PWT25_002568"
     CDS             3968..4531
                     /gene="grpE"
                     /locus_tag="PWT25_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL1022583.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4582..6411
                     /gene="dnaK"
                     /locus_tag="PWT25_002569"
     CDS             4582..6411
                     /gene="dnaK"
                     /locus_tag="PWT25_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL1022584.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
BASE COUNT         2230 a         1115 c         1346 g         1771 t
ORIGIN      
        1 aatataaata gagtgtatct tctttgataa atgtcagaaa aagtgtgaca aataatcatc
       61 agtaaaatta cttgtcacac tattgataag gagtatttaa ttgaagagaa tgattaggag
      121 aaaggatctc cgtacttttt atagtaatca tagaatccaa gtttgtatag acgaatcgtt
      181 ttttctctta tttcttctgt ttcaaaatgg actgcttgaa gaatcaagtc tgtgaattct
      241 ataggagctg ttccattcgc agtaatcaaa ttacgatctt tcacagcttg ttgttctata
      301 aaatcttctt tattctgata attttccgat tctaaccaag gaaattgcgc atttccggtg
      361 tgtttgtaac cttgcaataa tccgctctga gctaaataat caactgcccc acaaatagca
      421 gctactggta cgtctgtttt taacaattta tcgattattt gatataaatg atgatttttt
      481 atgtcccatg aattgccacc gatcataata aaaaggtcaa ctttttcagg tatttgttcg
      541 attaagtaat cgatttttgt ttttaatcct ccaatggaag tgacctctga ttggatagaa
      601 gcagttttgt tttgccattt ctcattttgg tttagtgtgc tagtaatata agcaccttcc
      661 caatcggcat attcctctaa tagaaacgta atcgcagttt tcattttcaa gctctccttt
      721 actatctata acaaggacaa cagttttaac ctacagacat tcattaaaat tgggtagcga
      781 cagcgaaaac aacaattctt aagttttaga cagagtatac agatagtcaa cttaattaaa
      841 tacgaactaa ataaaaatga caggtaatca tttttgttta ccagcattat aacattttgc
      901 atacatggat gacaaaagaa ccaattccca cttgcttgag tacaatatta aaagaaactg
      961 gaaggcaaaa agtaattata gaaacgaaaa ataaatcata tcgggcactc agacatgaaa
     1021 acaaccctag agatatataa ctaggtaacc agtttaatga aagaaaaaat catccaagaa
     1081 gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat gcgtatatga
     1141 gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg aaaatagctt
     1201 ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt accgtttatc
     1261 gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt ttagagctag
     1321 gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa cgcgatttta
     1381 agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca atgatagaaa
     1441 caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt tgcgagcata
     1501 tctgctatta ctgtgatttt aataaagttt ttttagaagg gcagccagta gatgagtatg
     1561 tggaaatgct gctgaatgaa atgaaaataa tgctagaaag tcatccgaca gcgaatttag
     1621 aaacacttta cgtagggggc gggacaccaa cttctttatc tgctaaacaa cttgatcgac
     1681 tgttatcagg tgctagagag atcctgcctt ttcaaaatgg taatgagttt actgtagaag
     1741 cgaatccagg tgatttgacc cgtgaaaaac ttcaagtaat gaaaaactat ggcgtcaatc
     1801 gtttatccat gggagtgcag acatttgata atcgcttatt gaaaaaaatt ggacgtaaac
     1861 acaccgcaga agatgtgtat caaactatgc aatttttaga agcagaaaat tttaccaatg
     1921 taagcatcga tttgatttat gcattaccgg gacaaacact agaaggatat ctggatactt
     1981 tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg atcctcgaaa
     2041 acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct gatcaggaaa
     2101 cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga cgtcaccaat
     2161 atgaaataag taactttggg ctctccggac atgaatctca acataacctc atgtactgga
     2221 acaatgacca ctattttggg tttggagctg gtgctagcgg ttatcttgga gatattcgtt
     2281 accgcaacaa aggaccaatc caacactatt tacgcccatt acgagcaaat gaacgcccgg
     2341 tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg ttcttaggac
     2401 taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa aaaacaattg
     2461 aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt gttaatgaag
     2521 aagaccgtgt ttatttgaca ccaagaggga cctttttggg gaacaatgtg tttgagcgct
     2581 ttttgtttga aaaatagttt aaagaatatt tgttttttat ctgaatttaa ataaaaaaag
     2641 ttcaaaaata caataaagga ctattgaaac gttgatataa ctggatttac atcaattatt
     2701 tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct tgacaggaga
     2761 agggcttctt gctatattta taagtggatt agcacttaga atatatgagt gctaatgaga
     2821 ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca tccaacacta
     2881 tacgagtagt ggcgtgcccg ttggttccaa aacattgatg tctgagggag tcaaggccag
     2941 ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc tgttgaagac
     3001 tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg tggatcattt
     3061 gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt ctctaggcag
     3121 agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat ctgagctgac
     3181 aagctatacg actttttcat tgggacctga aataaaagac cgccgactga ctggctttcg
     3241 tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata tggggaatgt
     3301 agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag aaaaaatggt
     3361 acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc ataaacttcg
     3421 tacggagatt ccgatgattc ttcataaata tttccaaacg acagaaggga tgtctcagtt
     3481 atttgacacc gttcttggtc atgtttttga agataaggtt tatgtcagtg gacaaatgaa
     3541 tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt tttcttttat
     3601 gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata ttcatatcaa
     3661 gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc aagcaagtta
     3721 cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta gtatgcctta
     3781 ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta aacttgctga
     3841 ttactaccgt tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct atttgcagct
     3901 gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat gaaaggaaga
     3961 ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg ctcagccaga
     4021 accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg aagcggaaga
     4081 attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagataaat acttgcgggc
     4141 tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc aattacaaaa
     4201 ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc tggaacgtgc
     4261 attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg tggaaatggt
     4321 cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc ctgctaaagg
     4381 agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga ctgaagatac
     4441 accagccgat acgattgtag aagtactaca agaaggctac aaattacatg atcgcgtgtt
     4501 aagaccaacc atggtcattg ttgcacaata attgtgcgga tataaaactt acactttcaa
     4561 ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg tacgactaac
     4621 tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc agaaggaaac
     4681 cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg ggaagttgca
     4741 aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca tatcggtgaa
     4801 gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat ctcagcaatg
     4861 atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt cgaaaaagca
     4921 gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa agatgctggt
     4981 aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc tgctcttgcc
     5041 tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct tggtggcggt
     5101 acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt gtcaacagct
     5161 ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta catggtagca
     5221 gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt acaacgcttg
     5281 aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac acaaatcagc
     5341 ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac attgacacgt
     5401 gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc agtacgtcaa
     5461 gcgctaaaag atgcaggttt atcacaatca gaaatcgacg aagttatttt agtcggtgga
     5521 tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa agaaccaaac
     5581 aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg tggcgtgatc
     5641 actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt aggtatcgaa
     5701 acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc aacaagtaaa
     5761 tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca tgtattacaa
     5821 ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt gacagatatt
     5881 ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga taaaaacggg
     5941 attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat cacgatcaaa
     6001 tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg taaaagatgc agaagcaaat
     6061 gcagaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt agatgcattg
     6121 ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga agaagaagtg
     6181 aaaaaagcag aaacagctcg cgatgaattg aaagccgctg ttgaagcaaa caacatcgaa
     6241 gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac tgttaaacta
     6301 tacgaacaag cggcacaaca acaagctcaa gaaaacccag aagcagctca aggcggagca
     6361 gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata atcagttcac
     6421 tgaatttcga gagaaggcca aagttgctac tttggctttt tc
//
LOCUS       DANJLF010000106         6455 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000106 DANJLF010000000
VERSION     DANJLF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            413..619
                     /locus_tag="PWT25_002570"
     CDS             413..619
                     /locus_tag="PWT25_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL1022585.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(821..1822)
                     /locus_tag="PWT25_002571"
     CDS             complement(821..1822)
                     /locus_tag="PWT25_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1022586.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1827..3740)
                     /locus_tag="PWT25_002572"
     CDS             complement(1827..3740)
                     /locus_tag="PWT25_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL1022587.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3908..4414)
                     /locus_tag="PWT25_002573"
     CDS             complement(3908..4414)
                     /locus_tag="PWT25_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1022588.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4574..5014
                     /locus_tag="PWT25_002574"
     CDS             4574..5014
                     /locus_tag="PWT25_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL1022589.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5173..>6455
                     /locus_tag="PWT25_002575"
     CDS             5173..>6455
                     /locus_tag="PWT25_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL1022590.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT         1832 a         1314 c         1047 g         2262 t
ORIGIN      
        1 tatttttgtt tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct
       61 tgataacact ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg
      121 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
      181 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      241 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      301 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      361 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      421 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      481 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      541 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      601 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      661 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      721 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      781 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      841 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      901 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      961 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
     1021 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
     1081 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1141 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1201 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1261 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1321 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1381 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1441 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1501 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1561 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1621 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1681 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1741 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1801 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1861 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1921 gttttcggca acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga
     1981 atcggttgcg tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct
     2041 gcattctctg tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa
     2101 ataatagttg gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat
     2161 tcgtatcctt cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc
     2221 tggatttcgt cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt
     2281 atctgatcat ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg
     2341 tcaacttcct gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct
     2401 cctgataact catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca
     2461 tggataatca atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta
     2521 tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca
     2581 tgctcttgat ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct
     2641 actgtctggc tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca
     2701 ataattcctt tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct
     2761 cccatcgaac caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt
     2821 atccagtaaa ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta
     2881 ttaccagtcg ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg
     2941 tattcttctg taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact
     3001 gttttatctg aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt
     3061 ttgtttagta cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta
     3121 ggcgttgtcc catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa
     3181 gcgatatcgt acaatagata agtagcaatc agatgtttga taatctcttt atctacacca
     3241 taatcattaa cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct
     3301 gttggtccat taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca
     3361 ttgaaatttt cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact
     3421 ttagcagaag aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg
     3481 ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt
     3541 tcagtatttg tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt
     3601 tctgtggttt ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc
     3661 cattgcggga cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt
     3721 tttgttctca agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa
     3781 cattttttat acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc
     3841 attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag
     3901 aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag
     3961 cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca
     4021 tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg
     4081 cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat
     4141 tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag
     4201 ataattccga caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt
     4261 gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg
     4321 ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat
     4381 cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg
     4441 atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta
     4501 cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct
     4561 attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg
     4621 cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca
     4681 accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga
     4741 aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag
     4801 tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat
     4861 ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct
     4921 ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga
     4981 ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttct
     5041 ggatctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta
     5101 ataaacgcaa acagacacct tgaaatcctt tagaataaag tcgacgaaaa ccattcaaag
     5161 gagtgtttca agatgtccta ttcagagttt accaaagagt tactagatat tctagaccta
     5221 aatcttacct ttcatgaaga tgcctttcgc aaagagcgca ttaatgacga aacatgtttt
     5281 gtttttgatg gcacattgac ctatcaacca gaggaatgct tccattgcca ctatcaaaat
     5341 aagcaaacga tcattaaatg gggctggaaa aaagtatcca tcttattgaa tgatgtcagt
     5401 aactacaaga ccattgtgcg gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag
     5461 acttttttag cagaagattc agttagcgat cgtcgctgct ccattgcccg cagagtcaaa
     5521 caagccattc ttgagctatt aagtgagcct atttctatgt ctttgattgc acgtatgaag
     5581 cacatttctc cgaccactgt cattcgaata cttcgcagcc ttcggcctaa aaccgtttct
     5641 ttgaaccagc cgttaccaga agtggtgtgt ttcgatgaat tcaaatcggt gaaaaacgtc
     5701 tcgggagcca tgagctttgt catgatggac ggcaagaccc atcaactgat tgatattgtg
     5761 gaaaatcggc aactcaatcc tttacgcgac tatttcttgc gctaccctag aaaagtccgt
     5821 gaacaagtac gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag
     5881 tgttttccga atgcccgaat cgtcgccgat cgctttcata ttagccaaca catcggtcgg
     5941 gcctttacca accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg
     6001 aagcacttaa agaaatactg gagactactt caaaagaatg cgtgggaatt gaatggtcag
     6061 catcgctact ggcgtccgtc atttcgagat catctgacag aagcggagat agtcgatcgg
     6121 ctattatatt atgatgactc cttaaagcga ggctacgaag tctaccaggt ttttctttcc
     6181 gctatcagaa gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag
     6241 ttgccagagc actatcaacc agtgttcacg accttcaaga aatatcgaac agaaatcaaa
     6301 cgagcgttgc gagttcccta ttccaacggc cctatagaat gtttgaacaa ccatatcaaa
     6361 gtactaaaac gaattgccta tggctttaga aactttcaaa actaccgaga acgaatcttt
     6421 ttatatcgag gaaaatattt caagaaaact aagaa
//
LOCUS       DANJLF010000107         6316 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000107 DANJLF010000000
VERSION     DANJLF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            33..338
                     /locus_tag="PWT25_002576"
     CDS             33..338
                     /locus_tag="PWT25_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022591.1"
                     /translation="MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKI
                     HSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(411..1058)
                     /locus_tag="PWT25_002577"
     CDS             complement(411..1058)
                     /locus_tag="PWT25_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL1022592.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1066..1752)
                     /locus_tag="PWT25_002578"
     CDS             complement(1066..1752)
                     /locus_tag="PWT25_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022593.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1758..2831)
                     /locus_tag="PWT25_002579"
     CDS             complement(1758..2831)
                     /locus_tag="PWT25_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1022594.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3053..3727
                     /locus_tag="PWT25_002580"
     CDS             3053..3727
                     /locus_tag="PWT25_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1022595.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3732..5072
                     /locus_tag="PWT25_002581"
     CDS             3732..5072
                     /locus_tag="PWT25_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1022596.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5221..6207)
                     /locus_tag="PWT25_002582"
     CDS             complement(5221..6207)
                     /locus_tag="PWT25_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL1022597.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1920 a         1196 c         1104 g         2096 t
ORIGIN      
        1 tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc
       61 attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat
      121 atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg
      181 ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga
      241 atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg
      301 caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta
      361 taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt
      421 ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa
      481 ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa
      541 ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat
      601 ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt
      661 cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac
      721 cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt
      781 aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc
      841 aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct
      901 gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt
      961 ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct
     1021 ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt
     1081 ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat
     1141 acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag
     1201 taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa
     1261 gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc
     1321 agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga
     1381 atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc
     1441 ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa
     1501 gcgtaaatct gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat
     1561 ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga
     1621 accagaagga ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt
     1681 caaagctgag acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa
     1741 tatttttttc atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta
     1801 cgtatcgaaa acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac
     1861 cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa
     1921 gcaaacaaaa gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa
     1981 gatttagcga tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc
     2041 ttctgaagtg tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa
     2101 aagtatatca tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg
     2161 aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct
     2221 gaatccttta ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt
     2281 ccatacttca aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa
     2341 taagtttcag gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc
     2401 gtatctccaa ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt
     2461 gttttaaatt catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg
     2521 aaaactgtaa cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt
     2581 gctttctctc ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat
     2641 acttgatttg catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt
     2701 ttaaactctt ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc
     2761 aagaacataa taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata
     2821 gctagaaaca tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt
     2881 gaatagtgta actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa
     2941 ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct
     3001 tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa
     3061 actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc
     3121 aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg
     3181 agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt
     3241 agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag
     3301 tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt
     3361 ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt
     3421 tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt
     3481 gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt
     3541 acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga
     3601 ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg
     3661 aagtactgga ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag
     3721 ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa
     3781 atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa
     3841 accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa
     3901 cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa
     3961 cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta
     4021 acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg
     4081 ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa
     4141 atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa
     4201 aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa
     4261 tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga
     4321 ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt
     4381 cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa
     4441 cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag
     4501 aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg
     4561 atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca
     4621 atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg
     4681 gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa
     4741 tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata
     4801 agatcatttt ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg
     4861 gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc
     4921 aatctcgggc gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa
     4981 tcgaacagca tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta
     5041 aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca
     5101 aaaatcttgt cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc
     5161 ggaaataagc cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc
     5221 ttacgatttt ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc
     5281 ttctcggcta acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc
     5341 aacttcttca cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat
     5401 agtaatcgtg tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg
     5461 tttgcgttcg atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt
     5521 gaccacatgt ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa
     5581 tttttcttct tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc
     5641 agtcttgctt gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg
     5701 tgtcatattc aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg
     5761 atctgttcca gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc
     5821 ttgtgtatcc attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac
     5881 aaattttcca tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc
     5941 cgtctcagaa tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg
     6001 gtcttcttca ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc
     6061 aaatcctagt ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata
     6121 cccttcgtta cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc
     6181 gatgaccgtt aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg
     6241 gtatattgtt acttataaat ttattctata ggagacgatt gttttctaca aatatttaga
     6301 ttggaatttg attaaa
//
LOCUS       DANJLF010000108         6303 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000108 DANJLF010000000
VERSION     DANJLF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            334..714
                     /locus_tag="PWT25_002583"
     CDS             334..714
                     /locus_tag="PWT25_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL1022598.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="PWT25_002584"
     CDS             696..1610
                     /locus_tag="PWT25_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL1022599.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="PWT25_002585"
     CDS             1855..2544
                     /locus_tag="PWT25_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022600.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="PWT25_002586"
     CDS             complement(2665..2751)
                     /locus_tag="PWT25_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL1022601.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="PWT25_002587"
     CDS             3427..4377
                     /locus_tag="PWT25_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL1022602.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="PWT25_002588"
     CDS             4398..4931
                     /locus_tag="PWT25_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL1022603.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="PWT25_002589"
     CDS             4947..5117
                     /locus_tag="PWT25_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022604.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="PWT25_002590"
     CDS             5567..6100
                     /locus_tag="PWT25_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022605.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2324 a         1102 c         1173 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtatacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DANJLF010000109         6287 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000109 DANJLF010000000
VERSION     DANJLF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..177
                     /locus_tag="PWT25_002591"
     CDS             <1..177
                     /locus_tag="PWT25_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022606.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="PWT25_002592"
     CDS             190..690
                     /locus_tag="PWT25_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL1022607.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="PWT25_002593"
     CDS             787..2634
                     /locus_tag="PWT25_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL1022608.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="PWT25_002594"
     CDS             2637..3533
                     /locus_tag="PWT25_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1022609.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="PWT25_002595"
     CDS             3583..3972
                     /locus_tag="PWT25_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1022610.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="PWT25_002596"
     CDS             3959..>6287
                     /locus_tag="PWT25_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1022611.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DANJLF010000110         6278 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000110 DANJLF010000000
VERSION     DANJLF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(182..1144)
                     /locus_tag="PWT25_002597"
     CDS             complement(182..1144)
                     /locus_tag="PWT25_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1022612.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="PWT25_002598"
     CDS             complement(1146..2585)
                     /locus_tag="PWT25_002598"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL1022613.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="PWT25_002599"
     CDS             2770..4716
                     /locus_tag="PWT25_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL1022614.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4980..5852
                     /locus_tag="PWT25_002600"
     CDS             4980..5852
                     /locus_tag="PWT25_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1022615.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6148..>6278
                     /locus_tag="PWT25_002601"
     CDS             6148..>6278
                     /locus_tag="PWT25_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HDL1022616.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYN"
BASE COUNT         2006 a         1086 c         1106 g         2080 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga attttaaaaa atgataaaaa
     4861 ttcaaaaaga aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta
     4921 aatatgatat atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta
     4981 tgctatacgg tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     5041 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt
     5101 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     5161 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     5221 aattggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     5281 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     5341 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     5401 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     5461 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     5521 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     5581 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     5641 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     5701 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     5761 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     5821 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     5881 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     5941 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta
     6001 tggaaatttc gatagccgta ggccacttgt attgtgcaat agttttttac acgccgtttg
     6061 tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaatcg taatacccgc
     6121 taaagcatac aaggagagtg aacataaatg acttataccc atcttacacc aaacgagctt
     6181 gtaatgatag aagcatattt tcatcaagaa actccggttg ctatcgttgc gaagcagctt
     6241 aaacgtggac gccaaacaat ttacaatgtc tataactt
//
LOCUS       DANJLF010000111         6173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000111 DANJLF010000000
VERSION     DANJLF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            725..811
                     /locus_tag="PWT25_002602"
     CDS             725..811
                     /locus_tag="PWT25_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL1022617.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PWT25_002603"
     CDS             complement(932..1621)
                     /locus_tag="PWT25_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022618.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="PWT25_002604"
     CDS             complement(1628..1906)
                     /locus_tag="PWT25_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022619.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="PWT25_002605"
     CDS             complement(1866..2780)
                     /locus_tag="PWT25_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL1022620.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PWT25_002606"
     CDS             complement(2762..3142)
                     /locus_tag="PWT25_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL1022621.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="PWT25_002607"
     CDS             complement(3592..3879)
                     /locus_tag="PWT25_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL1022622.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="PWT25_002608"
     CDS             complement(3899..4123)
                     /locus_tag="PWT25_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL1022623.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="PWT25_002609"
     CDS             complement(4578..4748)
                     /locus_tag="PWT25_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022624.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="PWT25_002610"
     CDS             complement(4763..5296)
                     /locus_tag="PWT25_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL1022625.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="PWT25_002611"
     CDS             complement(5317..6159)
                     /locus_tag="PWT25_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1022626.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DANJLF010000112         6110 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000112 DANJLF010000000
VERSION     DANJLF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(322..807)
                     /locus_tag="PWT25_002612"
     CDS             complement(322..807)
                     /locus_tag="PWT25_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022627.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(946..1161)
                     /locus_tag="PWT25_002613"
     CDS             complement(946..1161)
                     /locus_tag="PWT25_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL1022628.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1385..2185
                     /locus_tag="PWT25_002614"
     CDS             1385..2185
                     /locus_tag="PWT25_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1022629.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2204..4072
                     /locus_tag="PWT25_002615"
     CDS             2204..4072
                     /locus_tag="PWT25_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL1022630.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4065..4556
                     /locus_tag="PWT25_002616"
     CDS             4065..4556
                     /locus_tag="PWT25_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL1022631.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4544..5098
                     /gene="srlA"
                     /locus_tag="PWT25_002617"
     CDS             4544..5098
                     /gene="srlA"
                     /locus_tag="PWT25_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL1022632.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5116..>6110
                     /locus_tag="PWT25_002618"
     CDS             5116..>6110
                     /locus_tag="PWT25_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL1022633.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2162 a          968 c         1223 g         1757 t
ORIGIN      
        1 tttgaaaaag ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa
       61 ttcttctatc caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt
      121 aactcagttg accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa
      181 gtgagtatat tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat
      241 ttatactccg tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta
      301 aatccgtata atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga
      361 tccttcgggt gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg
      421 tttgctggaa gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat
      481 atcgtcactt tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt
      541 ttctaatgga agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa
      601 cgactgaata cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat
      661 ggaagtagta gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga
      721 ttctttaaca gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa
      781 caagcctgcg taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc
      841 agtatatcac ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa
      901 gggaaggttc atgaagataa taactttttt aataattata gctttctaat atctttcatc
      961 aacttttcta gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac
     1021 gatataaatg atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc
     1081 aagttcgaac aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc
     1141 aatagcatct aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat
     1201 caaaaagaga aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat
     1261 gaaaattttt tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg
     1321 tcttaatttt ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc
     1381 acatatgtcg gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc
     1441 aggaattgga agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt
     1501 tgatgtaact gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc
     1561 ttctaaaacg gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga
     1621 tggtctagtc aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc
     1681 aaaaagcaag tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa
     1741 aagtgtatat ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt
     1801 gatcgtgaat ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc
     1861 tgctacaaaa gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag
     1921 gaatgttcgt gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca
     1981 agaatatgaa gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg
     2041 ttacagtcga acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga
     2101 tctagtctgc tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat
     2161 ttcaggaggt aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag
     2221 atggtaccaa ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca
     2281 aaaagaaatc aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga
     2341 aattgccggt attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga
     2401 ttttgatttt tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc
     2461 ggcatccaaa agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg
     2521 tatggatgat ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa
     2581 gacattaaaa gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg
     2641 actaagaata ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc
     2701 ctacttttca gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt
     2761 tcagaagaag acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat
     2821 ttctttgaat agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg
     2881 gtgttttgaa tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga
     2941 actaactttg agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac
     3001 aagtataacg atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat
     3061 gatgcgtatt cgacgattag atctcattga tctggatgag tccttcttat atcaagagat
     3121 gaaacagcac ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt
     3181 attttatggt gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat
     3241 acaagcatta gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac
     3301 gttgtatttg gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc
     3361 cattgttcat acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat
     3421 attggggagg gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct
     3481 aaacaaatat ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc
     3541 ggtgatccat ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc
     3601 aaaaaaaaag cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga
     3661 aaaaaagacg tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta
     3721 tgtggatgaa gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt
     3781 taacaaagaa tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc
     3841 agtagggcta ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt
     3901 atttgccata cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga
     3961 tatcctattt gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca
     4021 agaatcattt ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc
     4081 tattcatagt tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac
     4141 taaagcattt caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta
     4201 aacgacctgg gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc
     4261 aagttttaga agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac
     4321 ctgtctatgc gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg
     4381 aagatcgttt gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg
     4441 caggaaatga aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc
     4501 ggatatggaa aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa
     4561 atttgctgag ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat
     4621 gacaggaatc gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt
     4681 tttaggtgaa gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg
     4741 ttaccttgtg ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat
     4801 ggctcgtttc cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg
     4861 tcatacgagc aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg
     4921 gattgctcaa gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt
     4981 gcttgtggga ctgctgatga actttattgg tggttggata actgatttta ctactggatt
     5041 tgtatgcaga cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag
     5101 aaaggagagt tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga
     5161 ggtcctttga ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga
     5221 ggtgagaggc caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat
     5281 gggttcaaaa catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg
     5341 actcttagat gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca
     5401 ggaaaaagtg gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg
     5461 ttagaacaaa tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca
     5521 tctccagata aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag
     5581 cagcaaaact ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat
     5641 caatcagcaa gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc
     5701 gtttcccttt tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt
     5761 atggcacctt tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg
     5821 ccatttttat ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg
     5881 atcggtgtag agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt
     5941 gctatcaata ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca
     6001 aaagctgaga cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc
     6061 atcccacgag ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DANJLF010000113         6085 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000113 DANJLF010000000
VERSION     DANJLF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..162
                     /locus_tag="PWT25_002619"
     CDS             <1..162
                     /locus_tag="PWT25_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1022634.1"
                     /translation="GLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYV
                     QEAFYSSLI"
     gene            complement(248..1342)
                     /locus_tag="PWT25_002620"
     CDS             complement(248..1342)
                     /locus_tag="PWT25_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL1022635.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1531..2292
                     /locus_tag="PWT25_002621"
     CDS             1531..2292
                     /locus_tag="PWT25_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1022636.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2407..2847)
                     /gene="fabZ"
                     /locus_tag="PWT25_002622"
     CDS             complement(2407..2847)
                     /gene="fabZ"
                     /locus_tag="PWT25_002622"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL1022637.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3067..3822
                     /gene="fabI"
                     /locus_tag="PWT25_002623"
     CDS             3067..3822
                     /gene="fabI"
                     /locus_tag="PWT25_002623"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL1022638.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3932..4945)
                     /locus_tag="PWT25_002624"
     CDS             complement(3932..4945)
                     /locus_tag="PWT25_002624"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL1022639.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5110..6030
                     /locus_tag="PWT25_002625"
     CDS             5110..6030
                     /locus_tag="PWT25_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL1022640.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1870 a         1206 c         1087 g         1922 t
ORIGIN      
        1 ggactgccga aatcaatgga ttttagaaaa gtgaatcaga catttatttc cagtgtcagc
       61 aatcaacgta atcatattcc aagaaaatca ttgaattaca gaacaccaat cgaaatattt
      121 ttgagctatg tacaagaagc attttattct agcttaattt gacaaatcat aacctataaa
      181 aaaacaaaat aaaaaagcag aaataactgg ctatttctag cggattattt ctgcaataac
      241 taattatcta ttttttattt tcaattaatt taaagcttga ccatggtttc aaaaagtcaa
      301 ggaggaataa ttcgtcatcg gagattcttc ctacaacatt gacacgtccg tcgtttttca
      361 tatccttcaa ggcgatttgt gtttctcctt tgtattgacc gtacccttca ttgtcaatca
      421 gtacatcccc atgatgcata tctcgcgtat tatgaggcgg gaaatcttta tctttgtagt
      481 atacacgagt catcgttgaa cgaaggatat attctgagcg atcccctcga taactatgaa
      541 ggttatcaaa caagcagatt cgttcgtttt ctgtaatacc ttcttccgta tctactttca
      601 atgttggata atccgcgtta aatgcttctg ccatttcttt caattcttct tcacttgcgt
      661 acgcatttcc aatagaaata tcatcaatca atcctgtaag gattaaatgc ttcacttgtg
      721 tcgcaatttc aagatctcgg tggtcttcca gcgtgcacaa gccatcttgc gtaggccaag
      781 ggccaaactc agcagattgc gaattgacga atgccatcgt attcaaatta tattttctga
      841 atttttctga acaaaaaaca aagtgttcgt atcctaaacc agagtatcga tgaggataaa
      901 aattatgaga acctaataaa ttatccgtgt ttggcgaata actcatgatg ttgtcaacgt
      961 aattcgtgcc cgaactcata ttgatctcga ttttgatacc aaaaggattg cgcgtcattt
     1021 ttgcttcttc tgctcctgtg aatccgatat ctaagcggac cccgtaggca cccatcttat
     1081 ggaaaaaaga taagtcatcg taagaaatct ccaactgttc aaataacgct ggattgatat
     1141 caaccattac ttccatgcct aggctattcg cgtaatcaac tacttcttta aattctgaaa
     1201 gaactttttc tttatcatca ttgatctgca gcaaactggt aaaaacccgt ttatacccat
     1261 acttatgagc taaatccaag tacgctttgt ccttttcaaa tgttgaccgt tctggataaa
     1321 tcgagattcc taattttccc atattaccat ttcctcctgc atcttttcgt ttttgtatcc
     1381 acttacatta tagcaaacac aaagtaaatg gtctacacct tttctttttc ttttaaaata
     1441 aatgataaat tccaataatt taagtaggta ttttccctct attttgttat cattaaagat
     1501 aacaaaatag taaattggaa aggaacagtt atgaagaaaa ataaagttat tttaagtatt
     1561 agtttagcag cagcattgct gtttgctgct ggttgttctg caaacaacac gacttcttct
     1621 agttcgtcta cagaaagttc aaaaagtaca tctactgcgg aaagcacgac tgccagtagc
     1681 gtcgatttga acgcattgga attaccacaa ttgacgaaag atgtcgccga aaatgaagat
     1741 ctggtcgaaa tggtgacgaa taaaggcaca atcaaaatca aattatttcc cgaacacgca
     1801 ccaaaagcgg ttgaaaactt tatgacacat gcaaaagacg gttactacaa cggactgact
     1861 ttccatcgcg tcatcaaaga tttcatgatc caaggtggcg atcctaaagg cgatggcact
     1921 ggaggggaaa gcatttgggg agaaggattt gaaacagaga tcaacaacca cctttataat
     1981 ataagaggtg cgttatctat ggctcgctca caagatccta acagtaacgg cagccagttc
     2041 tttatcgttc aaaatacaga tgatatgcat gatggtcttt taaaagacga ctatcctcaa
     2101 gcaatcatcg acgcttataa aaacggcggt tatccttcac tagatggcca ttatactgtc
     2161 ttcggccaag tgattgaagg aatggatgtc gtggatgcta ttgcaaatat cgaaacagat
     2221 agttcagaca aaccaaaaga agaagtaaaa atcgataaaa tcaatatact gcaagaagca
     2281 aaacaaaaat aaaaaaataa cgtctgaatc ctgcatagat gacaacgtat gctaggattc
     2341 aggcgtcttc tttttacata aaaagcaagt aagcttcctt ttgttttgct atatcaggtt
     2401 ttatagtcaa gcattttggt tgacgatgaa cgtgaactcg cactcacaga cttttttatc
     2461 atctacatat gctgcagata acgctgtacc gactgatccc ttcattttgg tgatttcaaa
     2521 atgacatttc agcacatctc ctggaacaac tttttgacgg aatttggcct tattgatccc
     2581 accgatataa gctctttttc ctagaaactc ttctgatttt aggataagta tcgaacctgc
     2641 ttgtgccagt gtttctagaa tcaacgcacc cggcatcact ggattgtcag gaaagtgacc
     2701 ttggaaaaaa tcttcattga ttgttacatt ttttgtcgca acgatctttt ttcctggttc
     2761 taaagtatct acataatcaa tataacaaat aggataacga ttaggaatca attccattac
     2821 ttcagtagca gataatacgt aagacataat taaactcctt ctttcaggca attgccattt
     2881 tcattacttt gactatcaaa gtatcatatt cctaaatatt tagcaatctt tttttaattt
     2941 aagaaaaaat agtttgacaa tcaaaacatt ttgactaata attaagaaat caagaagtta
     3001 ttttagctat tattctctat ttttttacga ctatcaaatt tttatcagaa acaaaggaga
     3061 gtcattatgt catttctaga aggaaaaaaa atcgtcatta tgggcgtcgc taacaaaaaa
     3121 agcattgctt ggggttgtgc tgaagcaatg ttgaaacaag gggcagaaat tatctatact
     3181 taccaaaatg aacgaatgaa aaaatcatta gtcaaattgg tcggcgaaga agcattcact
     3241 gtagaatgtg atgtttctag cgacgaaagc atcgaaaaag caatgaatgc catcggtgaa
     3301 tatgctgggg agatccacgg ccttgtccat gcagttgctt acgccaacaa agaagaattg
     3361 tccggaaatg tgtctgatat ttccagagac ggctatcaat tagctcaaga tatcagcagc
     3421 tactctttaa ttgctgtatc aaaatacgca caaccgtttc ttgcaccaaa cagcgggatc
     3481 gttagcatga cttatctagg ttcagaacgc gcaatcccga attataacat gatgggtatt
     3541 gcgaaagctt ctcttgaagc tgctatccgt tatttggcag ctgagttctc gcctaaagga
     3601 attcgggtca atggaatctc agctggggca atcaaaacat tagctgtcac aggtgttaaa
     3661 gactatcaaa aactgatcca gttatctgaa tctcgtacgc ctgatcagca aggtgtgacg
     3721 atcgaagaag tcggtaatgc ttgtgctttc ttaatcagcc cactttccag cggaatcatc
     3781 ggtgatatta tctacgttga caaaggtgtc catctttcat aaacttttgt tgcattcttt
     3841 aaagtaaaac atataaaaat caggaaaaaa ctttttaaag tctcttcctg attttttctt
     3901 tttacgtttt tttcaactca ttactttgaa attattccac gacttcaatc cagccttctg
     3961 gtgcttctat gtctccaaat tgaatacctg tcaattcatc atacaaccgt tttgttactg
     4021 gtcctacttc cgtttcacta taaaatacat gaagattgtt attgtgataa atcccaccga
     4081 ttggagaaat aacagccgct gtaccacatg caccagcttc tgcaaattga tctagatcat
     4141 caatgaaaat atctccttca attgcttgca gtcctagtcg ttcttcagct aactgcaaca
     4201 aggaatattt cgtgatactt ggtaagatag agggcgattg cggagtgatg aattttccgt
     4261 cttttgtaat tccaaagaaa tttgctgcac ctacttcttc gattttcgta tgtgtggctg
     4321 gatcaagata aacacaatct gagtaatcca gttcttttgc ttctgttcct ggaagtaaac
     4381 tcgctgcgta atttccgccg acttttgctg caccagtccc cttaggagca gcacgatcgt
     4441 aatcagatac aataaaattc gttggtttta gtccaccttt gaaatacgct cctactggcg
     4501 tacagaagac gacaaataaa tattctggag ctggatggac accaatgttt tcaccgaccc
     4561 caatcagtaa agggcgcaag tataatgtag cgcctgttcc atagggcggc acaaaatctt
     4621 catttgcttt taccacttgc ttgacagcat caacgaacat cttttcaggg accattggca
     4681 tcagcaatcg tctagcactt tgattcatcc gtcgactatt ttgttcaggt cgaaagagat
     4741 tgaccttgcc gtcttttcga cgataagctt ttagaccttc aaaacattgc tgaccataat
     4801 gaagcgctgg agagccttca tgaatcgtca ggtaattatc ttctgtcaat tctccttctt
     4861 cccattgtcc gtctttccat ttggcaataa aacgccaagg agttttgata taagaaaaac
     4921 ctagattgtt ccaatcgatt tgcatatact taccaccttc gttattttct ccacatttta
     4981 tcattatcca tattatttgt cattttattt tcagcttttt tccttcatta tagttatttt
     5041 tgctatttcc ttttttgaaa acttcttggt acaatacaga aagaatcaaa ttttagaaaa
     5101 gaggatttta tgttttcatt attagcaact gccggtacga cagactcttc tggcgagaca
     5161 ttgacagatc aagctgtcag caaagtaaat gcgtttcagc ggttttggga cgggattgat
     5221 tgggacgcca ttttggctgc ctttatccaa aaaagtttat atctgatttt tttgatcgtc
     5281 cttttttgga ttcttcaccg tgtgggaaaa tatctgattg ataagtcttt taatcagtat
     5341 tctaaaaaag ccattctaaa cgagagccgc ttaaagactt tacatagtct tttaaatact
     5401 atttttcatt atacccttgg tttctttttt atctatgcaa ttttatctgc tatcggtgtg
     5461 ccgatcggat cacttttggc tggtgccgga attgcaggtg tggcaatcgg tcttggtgcc
     5521 caagggttca tgagtgatgt tattaccgga ttttttatta tcatggaaca acagatggat
     5581 gttggggact acatcaagct agcaaactta acgattgaag gaaccgtcgt atctgttggt
     5641 atccgtaccc ttcagctgaa agcaacggat ggtactgtcc atttcatccc gaatcgaaac
     5701 atcaccacga tcagcaatct ctctcgtgca aatatgcaag tacttttaga tattcggatt
     5761 gttcctgaag aaggatatga caaaatctat gagatcattg atcgagtaaa tcgtacatta
     5821 gctgaaaaat accaagaaga gctccaaact gaaccttcta tctttggatt agtggatctt
     5881 ggaaacagca attttgcgat tcgtacggtc tgctacgcat tgaacggaaa acaatatgcc
     5941 ttaaaagaag agttcttgag ctcctatgtc aaagaattga cagcttctgg tttcacgatt
     6001 ccgaacagtc caattgctgt cggaaaataa tttggacaaa cgatgcaacg aatctcacta
     6061 aaaagagcgt gggacaaaag taaag
//
LOCUS       DANJLF010000114         6001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000114 DANJLF010000000
VERSION     DANJLF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..395
                     /locus_tag="PWT25_002626"
     CDS             <1..395
                     /locus_tag="PWT25_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022641.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="PWT25_002627"
     CDS             398..1228
                     /locus_tag="PWT25_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL1022642.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="PWT25_002628"
     CDS             1244..2095
                     /locus_tag="PWT25_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1022643.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="PWT25_002629"
     CDS             2092..2451
                     /locus_tag="PWT25_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL1022644.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="PWT25_002630"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="PWT25_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="PWT25_002631"
     CDS             2624..2929
                     /locus_tag="PWT25_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022645.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="PWT25_002632"
     CDS             2929..3240
                     /locus_tag="PWT25_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL1022646.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="PWT25_002633"
     CDS             3237..3410
                     /locus_tag="PWT25_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022647.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="PWT25_002634"
     CDS             3407..3823
                     /locus_tag="PWT25_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL1022648.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="PWT25_002635"
     CDS             3823..4491
                     /locus_tag="PWT25_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL1022649.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="PWT25_002636"
     CDS             4488..4865
                     /locus_tag="PWT25_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022650.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="PWT25_002637"
     CDS             4862..5161
                     /locus_tag="PWT25_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022651.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="PWT25_002638"
     CDS             5151..5309
                     /locus_tag="PWT25_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022652.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="PWT25_002639"
     CDS             5403..5819
                     /locus_tag="PWT25_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL1022653.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DANJLF010000115         5809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000115 DANJLF010000000
VERSION     DANJLF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            450..1604
                     /locus_tag="PWT25_002640"
     CDS             450..1604
                     /locus_tag="PWT25_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL1022654.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="PWT25_002641"
     CDS             1598..2020
                     /locus_tag="PWT25_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022655.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="PWT25_002642"
     CDS             3143..4075
                     /locus_tag="PWT25_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1022656.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="PWT25_002643"
     CDS             4092..4607
                     /locus_tag="PWT25_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL1022657.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="PWT25_002644"
     CDS             4903..5184
                     /locus_tag="PWT25_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022658.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="PWT25_002645"
     CDS             5186..5602
                     /locus_tag="PWT25_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022659.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DANJLF010000116         5790 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000116 DANJLF010000000
VERSION     DANJLF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(99..1229)
                     /locus_tag="PWT25_002646"
     CDS             complement(99..1229)
                     /locus_tag="PWT25_002646"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL1022660.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="PWT25_002647"
     CDS             complement(1877..3079)
                     /locus_tag="PWT25_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1022661.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="PWT25_002648"
     CDS             complement(3139..3342)
                     /locus_tag="PWT25_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL1022662.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="PWT25_002649"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="PWT25_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="PWT25_002650"
     CDS             4879..5691
                     /locus_tag="PWT25_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022663.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DANJLF010000117         5614 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000117 DANJLF010000000
VERSION     DANJLF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(59..2008)
                     /locus_tag="PWT25_002651"
     CDS             complement(59..2008)
                     /locus_tag="PWT25_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL1022664.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="PWT25_002652"
     CDS             complement(2050..3108)
                     /locus_tag="PWT25_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDL1022665.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="PWT25_002653"
     CDS             complement(3227..3682)
                     /locus_tag="PWT25_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022666.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="PWT25_002654"
     CDS             complement(4266..5069)
                     /locus_tag="PWT25_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1022667.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DANJLF010000118         5220 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000118 DANJLF010000000
VERSION     DANJLF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..1256)
                     /locus_tag="PWT25_002655"
     CDS             complement(<1..1256)
                     /locus_tag="PWT25_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL1022668.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="PWT25_002656"
     CDS             complement(1270..2487)
                     /locus_tag="PWT25_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1022669.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="PWT25_002657"
     CDS             complement(2477..4072)
                     /locus_tag="PWT25_002657"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL1022670.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="PWT25_002658"
     CDS             4197..4982
                     /locus_tag="PWT25_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HDL1022671.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DANJLF010000119         5151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000119 DANJLF010000000
VERSION     DANJLF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            201..1916
                     /locus_tag="PWT25_002659"
     CDS             201..1916
                     /locus_tag="PWT25_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022672.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1919..3682
                     /locus_tag="PWT25_002660"
     CDS             1919..3682
                     /locus_tag="PWT25_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022673.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3722..4258)
                     /locus_tag="PWT25_002661"
     CDS             complement(3722..4258)
                     /locus_tag="PWT25_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL1022674.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(4581..5003)
                     /gene="mgsA"
                     /locus_tag="PWT25_002662"
     CDS             complement(4581..5003)
                     /gene="mgsA"
                     /locus_tag="PWT25_002662"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL1022675.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1547 a         1030 c          991 g         1583 t
ORIGIN      
        1 tcctatgaat gaagagactg gcatgttttc tcattttctt ttttttaaaa aaaatagtct
       61 attcaatttc taaagcttct tcataataaa actttcattt atgaaaaggc ttgttctctt
      121 ttcattctaa atttcctaag ctacaatgtg gtagtacttg ttttttcaat tgtggacaaa
      181 taaataatag gaggaatcat atgattcagt tattacgtta tgcaaaagac tatcgtaagc
      241 aaatcatttt gggcccgttc ttcaaatttc ttgaagctgt cttcgaactt gtcctgcctt
      301 taatgatggc ttccttgatc gataatggac taaaaatgaa tgaccgagga aaaatcattg
      361 aaatgggctt atggatggtc gctatgtctg tgattggtct gatttgtgct atcatttgcc
      421 aatactatgc ttcagtagct tcacaaggat ttggtacaga gttacgtaat cagctaatca
      481 aaaaaatcaa tactttctct cacaacgagt tgaatcattt tggaacagat accttgatca
      541 cccgaatgac aaacgacatc aatcagcttc agctagcttt agcaatggtc attcgtttat
      601 tgatcagagc accgttttta agtatcgggt cagtggtcat ggcttttgtg atcgactggg
      661 aagtaggtct gtttttctta gcattattgc ctattttctc aatcatttta ttttttatca
      721 tcaaaaaaac cgttcctctc taccaaaaag tgcaagaaaa actagatcag ctcaatgaac
      781 acgttagtca gaatcttagc ggtgttcgtg tcatccgcgc atttgcgcag acaaaaaaag
      841 aaacagagaa gttcgataaa agcacaaatg atctagcgaa caattattta cgtgtttcca
      901 atatctctgc cctattatca cctgctacaa ccttgatcat gaatgtcggg atcatctgtt
      961 tgctgacagt tggcgggatc aaagtaaata ttggtagctt gcaacaagga caagttcttg
     1021 ctttgatcaa ttatatgaat caaatgcttt tggcactgat cgttgtttca aacctcgtcg
     1081 tgatcttcac acgtgccgaa gcttcaggaa atcgggtgaa agaggtgcta gacacagaaa
     1141 attcgattct tgaagctgag gtcagtacta ctcccgattt ttcttctgaa gcaattatcg
     1201 cttttgacca tgttgacttc cgttatactc ctgattctgg tctttctctc caaaatatta
     1261 catttcagct gaagcgaaat acagtccttg gaatcattgg tccaaccgga agcgggaaaa
     1321 cgactttgac acaattgatt ccgcgtttct atgatgtcag ttccggtaca gttataatgg
     1381 acggaatgga tgttcgttct tggcagctgg atcatctccg tcagcaaatt gctcttgttc
     1441 cccaaacttc tgtactattt accgggacta ttcgtgaaaa ccttcaatgg ggtaagaaag
     1501 acgccacaga aaaagaatgt tgggaagctc tgaaaatcgc tcaggcagaa gaatttgtcc
     1561 gtcagcttcc aaatggttta gatacaaaag tcatggaaaa tgggaaaaat ttctctggcg
     1621 gacaaaaaca gcgactgacg attgcaagag ctttgattgc caagccagca ttactgattt
     1681 tagatgactc tttaagtgcg ttggattatc aaacagactt ggatttgcgt aaggcgctac
     1741 agacttatct tggcagtaca gtgattatta tctcccaacg tgtccgctct attcaagaag
     1801 cacatcatat cttagtgatg gatcaaggta agattgctgc tcagggtacc catgaagaat
     1861 tacttgctca atcagcagaa tatcaggaaa tcgtcgcatc acaggaggaa gaatagttat
     1921 gaaagtaaaa aaattatcct tgcagacgct ccgccgattc attccttacc ttactgccta
     1981 tgcaaaagac atttggctag ctgtgatact aggaatcatc aatgggattg caacagttgt
     2041 gatgacgtta caaattggtc aaagtatcga tcaaatgatt ggagaaggac aggtccgctt
     2101 caatcgactg ttccatttac ttattctatt cggtggtatc gttttaatca acgtagtcag
     2161 tcaatggctc atccaagtgt taagcaatcg gttagcttac ttatccgttg caaaattaag
     2221 aaaagatgca tttgctcatc tgaatcgatt gccacttcgt tattatgatc aaaactccca
     2281 cggaaatatc gtcagtcgat ttacaaatga tatggacaat atttccattg caatttctgc
     2341 ggccttcaat caattatttt ctggagtggc aatcatcatt ctttcgttta tttttatgat
     2401 gcgtctaagt ccacttctga cattggtcgt tctttgttct acaccaatta ttttccttgt
     2461 gagttggatc gttgctcgtg cttcacaaac aaatttcgat agacagcaaa ggattattgg
     2521 ggatatctcg ggctttgtga ccgaacgaat cggtaaccag aaaatcgtga aagcttttca
     2581 gcaagaagaa gctagtcaac agcaatttga actgctaaat gctgatctat atgaaaaagg
     2641 acaacgggca caattttctt cttccttgac aaacccgtct tctcgttttg tagatcacct
     2701 tgcttattta tcgattggtt tagttggcgg cctattggca ttgaagagtg gttcgtcaat
     2761 cactgttggt gtgatttcca gctttacgat ctactcaagt cagttttcta aaccatttat
     2821 cgaactttct ggaatcacga cacaactgca aaccgctttt tctggtctag aacgagcatt
     2881 tgatttgatc gatcagccag aagaaaagcc tgatttgcca gatgcctatg ccttggaacc
     2941 caaaaatatc aaaggaaaag tctcttttga tcatgtttct ttttcttatg accccaagca
     3001 gcgtttgatc gaagatttca attttactgc ttatccaggt caaaccattg ctatagtagg
     3061 aaaaacaggg gccggaaaat ccacacttgt taatttgctg atgcgttttt atgacacaac
     3121 aaaaggaaca attacagtcg atgaccatga catccgctct atccaacgtg atacattacg
     3181 taaaagtttt ggaatggtgt tacaagatac ttggctattt gacagtacat tgcgagaaaa
     3241 tcttcgtttt gggaatccgg aagcaagtga cgcagaaatc aatgatgccc taaaagcctc
     3301 ttatatgtat gaatttgtag aacgtttgcc ggaaaaatta gatactaaga ttggcggtca
     3361 agggttaaag atatcagatg gacaacgcca actgttgacg attgctcgaa cgatgatcag
     3421 taatccccct atgttgatct tagatgaagc taccagctca gtcgatacat tgacagagaa
     3481 aaaaatccaa caagccttct tagcaatgat ggaaggaaaa accagttttg tgattgccca
     3541 tcgcctgtcc acaatcaaaa atgcggacca gatattagtg atggatcagg gagctattgt
     3601 cgaaatgggc acccatgatg agctgcttca aaaagatggt tattaccgac ggctctatca
     3661 agcacaattc acagcacaat aaaaacaagc agtcttctgg gatgggaaga ctgcttgttt
     3721 tttatttagc gtggatcgct aaacttctta aaattttttt gtaaggaata ctacgtccta
     3781 cgtaataaat ggcaattgat tgaatcggta accatattac tgtttttaac aggcggggcg
     3841 cccaaacgac ccagctgaac aatggcacat ggtacatcaa cgcaagccat aatggtgtca
     3901 aaaataagtt gatgatcagc gtgacactaa gcgtagccag aatggcattt ttccaagtga
     3961 tctcttttcg atagaaaaag aatccgtaga ttgctccttc aataaatgca ttcaatgtaa
     4021 atccaataaa gaatgcagat ttcgagaaga gggccattcc tactaaatca gctaaaactg
     4081 cgcctatccc tgaccaaaaa ggaccaaaaa gaatgcccat gatcacttgt ggaataaatg
     4141 tgacactgat tttaataaat ggggtctcga ttgctaccag tcgggacaaa gtgaccatca
     4201 atgcaatcaa taagcccata atagcaatca tacgtgcgtc taaccggtgt ttcttcattt
     4261 ttagcatctc ctttaagagc tgctacatcg tatcatgcag cgaatgcgaa aataaaaccg
     4321 cgaacattac gtccttcgtc caagaggcaa catcccatcc tcttagcact taacgcttta
     4381 ttttctactc tgtattattt ttgggatctt ttcccacaga aactatagca tattttcaca
     4441 tttactgcaa atacgaaaaa ctaatattat tgtttatatt atccaatacg ttcgtattat
     4501 atcgcatatt atcagtttgt ttagctttta tagctacata taggcgaaca ttaaacgtta
     4561 tttttccata cgcttatcca tcaaaatgaa agtggatgct tgtctatttc atggacgatt
     4621 cttcgaaaat ctaggaagcc ggcttccagt cctcttagta gcacctcagc tgttccgata
     4681 ttcgtagcta acggaatctc atagacatca gataaacgga taagtgctgt tacatctggt
     4741 tcatggggct gtgcagccag cgggtctcga agaaaaatca ccaaatctaa ctgatcctcc
     4801 gaaatcatgg ctccaatttg ctgatccccg cctaacggtc cggatttaaa ccgatggatc
     4861 ggcaagccgg ttgcttccgt gatcctctgc ccagtcgtcc cggtagcaaa aagttcatgt
     4921 ttttccaata ttgttttata cgctgttgct aatttcacca tcataggttt ttttcggtca
     4981 tgagcgatca aagcgatttt cataagagac actccttttg atattgttaa catacttatt
     5041 tactccagtg tatcagtttt tatttcttat ggatatttat taaagcttaa aactagcaca
     5101 ttgacaaaaa aaagaaaaaa ttcttgttca acatgacatt gaagttagtt a
//
LOCUS       DANJLF010000120         5064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000120 DANJLF010000000
VERSION     DANJLF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            175..519
                     /locus_tag="PWT25_002663"
     CDS             175..519
                     /locus_tag="PWT25_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL1022676.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="PWT25_002664"
     CDS             520..939
                     /locus_tag="PWT25_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL1022677.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="PWT25_002665"
     CDS             complement(1031..1381)
                     /locus_tag="PWT25_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022678.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="PWT25_002666"
     CDS             complement(1347..2567)
                     /locus_tag="PWT25_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL1022679.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="PWT25_002667"
     CDS             2933..3499
                     /locus_tag="PWT25_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1022680.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="PWT25_002668"
     tRNA            3644..3731
                     /locus_tag="PWT25_002668"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..4631
                     /locus_tag="PWT25_002669"
                     /pseudo
     CDS             4065..4631
                     /locus_tag="PWT25_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            4744..>5064
                     /locus_tag="PWT25_002670"
     CDS             4744..>5064
                     /locus_tag="PWT25_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1022681.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT         1854 a          853 c          959 g         1398 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaacgaga gtataaaata ttttgtgtaa atgaaaaaat ccatacaaaa
     4681 aaggaagtcc cttctgtaga ataaagttaa cgacaaccaa ttcacagaaa agaggacttc
     4741 cctatgaatg attttactac agaaattgtg caaactctag tcactaaagg cgatttaaat
     4801 gaattattcc gttcgcactt agaaaaagcg ataaacacac tcctacggac tgaattaacg
     4861 gcttttttag attacgaaaa atatgatcgc actggtttta attcaggtaa ttcgagaaac
     4921 ggttcttact ttcgatcaat caaaaccgaa tatggtgaat taacattgga aatacctaga
     4981 gatcgtaatg gtgagtttaa acaacaaact ttaccagcct acaaaagaac aaacgataca
     5041 ttggaaacca ctattatcca ttta
//
LOCUS       DANJLF010000121         5009 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000121 DANJLF010000000
VERSION     DANJLF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..182)
                     /locus_tag="PWT25_002671"
     CDS             complement(<1..182)
                     /locus_tag="PWT25_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1022682.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="PWT25_002672"
     CDS             517..951
                     /locus_tag="PWT25_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1022683.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="PWT25_002673"
     CDS             974..1294
                     /locus_tag="PWT25_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022684.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="PWT25_002674"
     CDS             1435..1626
                     /locus_tag="PWT25_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022685.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="PWT25_002675"
     CDS             complement(1697..1954)
                     /locus_tag="PWT25_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL1022686.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="PWT25_002676"
     CDS             2112..2375
                     /locus_tag="PWT25_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022687.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="PWT25_002677"
     CDS             2399..2587
                     /locus_tag="PWT25_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022688.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="PWT25_002678"
     CDS             complement(2670..2855)
                     /locus_tag="PWT25_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022689.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="PWT25_002679"
     CDS             3070..3441
                     /locus_tag="PWT25_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1022690.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="PWT25_002680"
     tRNA            complement(3591..3663)
                     /locus_tag="PWT25_002680"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="PWT25_002681"
     CDS             complement(3709..4137)
                     /locus_tag="PWT25_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022691.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="PWT25_002682"
     CDS             complement(4197..4955)
                     /locus_tag="PWT25_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL1022692.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1744 a          784 c          978 g         1503 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttatt
//
LOCUS       DANJLF010000122         4876 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000122 DANJLF010000000
VERSION     DANJLF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(74..2308)
                     /locus_tag="PWT25_002683"
     CDS             complement(74..2308)
                     /locus_tag="PWT25_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1022693.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2787..3053
                     /locus_tag="PWT25_002684"
     CDS             2787..3053
                     /locus_tag="PWT25_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL1022694.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3053..4780
                     /gene="ptsP"
                     /locus_tag="PWT25_002685"
     CDS             3053..4780
                     /gene="ptsP"
                     /locus_tag="PWT25_002685"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL1022695.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         1494 a          916 c          988 g         1478 t
ORIGIN      
        1 attttgactt ttctgaaaaa agaatgagac ggcatttctc aaagaaatac cgtctcacgc
       61 tataaaactg gatttattca gccgtttctt cggctgattc tttagccaaa cgttctggtt
      121 tagaagtaac gatgattttt ccatctttat ccagttttgc tttcaactca tggatagaag
      181 ggtgatcaag ataaaattca gcgataccat cttcgatttg ttcttggatc gtgcgtcgaa
      241 gaggacgagc acccatagat ggatcatagc ctaagtcaac taatttttct ttgacattcg
      301 taggaacttc gatgtgaagt tgctgtgctg ccagcatttg gttgacatcg tctagcatta
      361 gtgtgacgat tgtcatcaag ttttcttttg ataatgcact gaattcgatg atcccgtcaa
      421 aacggttcag aaattctggt gtaaagtagt tgtttaattg gttcaataca gaacgagtaa
      481 caccttcgcg tgccgcacca aaaccaacgt ttgcttctac tttaccagtt ccggcattac
      541 ttgtcataat gatgatcgta tctttaaagc tgaccgttct tccttgtgca tctgtcaatc
      601 gaccatcatc taagatttgc aagaacatat gcaaaacatc tggatgagct ttttctactt
      661 catctaacaa gacaagactg taaggatttc tccgtacttt ttctgttaat tgaccagctt
      721 cttcgtatcc gacataacct ggaggagaac caatcaattt agagacacta tgtttttcca
      781 tatattcaga catatcgaaa cgaatcattg aatcttgtga accgaatagt tcgtaggcaa
      841 gttgtttcgc aagctctgtt ttacctacac cagttggtcc aacaaataag aaggagccga
      901 ttggacgatt ctgtttgttt aatccaacac ggtttcggcg aatcgctttc gctactcgat
      961 ctacagcgtt atcttgtccg atgacatgag ctttcaagtc atctgctaag tttttcagtt
     1021 gtgtctgttc tttttctttt aattcaccca ctggaatacc tgtccgttgt tcaacgattt
     1081 tttccatatc tttttcggta atcacaggtg tttcctcttc agtgagctga cgttctttca
     1141 ttttttgaag tttattgatc tgatcgcgat aataagctgc tttttcgaaa tcttcttctc
     1201 gagaagctaa aactttttgt tcttctgctt cttgcagttt tttctcgatt gttttaggat
     1261 caacgatttg gatcgtcaag tttttcttag aacctgtttc atctagcaaa tcgatggctt
     1321 tatccggcaa gaagcgatct tggacgtaac gattggataa agttgcggct gcttcgatgg
     1381 cctcatcagt atattttaca tgatgatagt cttcataacg tttttgcaaa cctttcaaaa
     1441 tactgatcgt ttcttcgaca gttggttcat ctacccgtac aggttgcata cgacgttcta
     1501 aagcagcgtc tttttcgatg atgcggtact catttaaggt agtagcacca accatttgca
     1561 attctcctcg agctaaagct ggttttaaaa tattaccagc atccatattt ccatcaccag
     1621 cagaaccagc accgacgatt tcgtgaactt catctatgaa taggatcaca ttttctgctt
     1681 gacggatctc ttcaatcaat ttttgcatac gttcttcaaa ttgtcctcgt ataccagtac
     1741 cttggactaa agatacgaca tctaaacgaa tgacttcttt gtccattaat ttttgcggta
     1801 catcaccatc tacgattttt tgtgctaaac cttcaacaac tgctgtttta ccaacacctg
     1861 gttctccaat caagacagga ttgttcttgg ttcggcgatt taaaatttca atgactcgtt
     1921 tgatttcgtc atcgcgtccg atcacaggat cgatatcacc ttgtcgtgct tgttctgtga
     1981 tattgattcc gtattctccc aataaacctt ctgtattgcc ttgaggtggc tgaccaccat
     2041 tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg ctcataattt ccttgctgtt
     2101 gcatttggcg agacatggaa cggaaaagat cgtccaaact gccaaatcca aatggatcat
     2161 tttgggccat attagtcaga cctccatttt gttgattttt gattttttga taacagctct
     2221 gacagtaatc gagttgtgtc cgctgaccat tgacagtggc atacaagtga atcgttgcct
     2281 cattcttgcc gcagttttga caaagcatga atcattcacg tcctttcaca aaaatgggta
     2341 catccatatt ttacatctcg tttttcatga ggtaaaagaa taacccctag ttttttaaga
     2401 gatttatgag gtagaaaagg tgaaatctct attgaccatt cctgaccttt gactcttatt
     2461 atactaaaaa taaaaaggga agcaagtaat tcgtttcata tgaaaacaaa gagggtcaaa
     2521 ggaaagaatg ataaacgtca ggagaagaga aagcgacaag aaaaaggaaa taaacacgtt
     2581 tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga ataatttgaa tacttttatc
     2641 tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat gttaatcttt acagatgaaa
     2701 gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga aaaccctcac ataaaaacaa
     2761 cactcactta aaggagaccg attaatatgg aaaagaaaga atttcacgta gtagcagaaa
     2821 ctgggatcca cgctcgtcca gctacactat tagtacaaac agcaagcaaa tttaactctg
     2881 atgtaaactt agagtataaa ggtaaatcag taaacttgaa atcaatcatg ggcgttatgt
     2941 ctttaggtgt aggtcaaggt tctgatgtta ctattactgc agaaggtgct gacgaagctg
     3001 atgcaatggc agcaatcgtt gaaacaatga agaaagaagg cttatctgaa taatggttga
     3061 aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt gcaaaagctt acctgctagt
     3121 tcaaccggat ttaacattca gtaaggcaac agtagaagat actgcggctg aagaagctcg
     3181 tttggatgct gcattagcta aatctactga agaattgcag caaattcgtg aaaaagcagc
     3241 gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc catttgatgg ttctttcaga
     3301 tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac aacagcgtaa atgctgaatc
     3361 agcgctaaaa gaagtaacgg atatgtatat cggtatgttc gaagcaatgg aagataatgc
     3421 ttatatgcaa gagcgtgctg cagatattcg tgacgttgct aaacgtattt tagcgcactt
     3481 attaggcgtt actttaccaa atccatcaat gatcaacgaa gaagtggtcg ttgttgcaca
     3541 tgacttgact ccaagtgata cagcacaatt agatcgcaat tttgtaaaag cgtttgtaac
     3601 agacattggt ggacgtactt cacattctgc tatcatggca cgttctttag aaattccagc
     3661 aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa ggcgatattt tggctgtcaa
     3721 cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa caaaaagctg aatttgaaaa
     3781 agcaggtgca gattacgcag cacaaaaagc agaatgggaa aaattaaaaa atgcagaaac
     3841 tgtcacagct gacggcaagc attttgaatt agctgcaaac atcggaacac caaaagatct
     3901 agttggtgtc cataataatg gtggagaagc agttggtctg taccgtactg aattcctgta
     3961 tatggattct ccagacttcc caacagaaga tgaccaatat gaagcttaca aagctgtatt
     4021 agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg gacatcggtg gagataaaga
     4081 actaccatat ctgcaattac ctcatgaaat gaatccattc ttaggatatc gtgcattgcg
     4141 tatcagttta tctgaacaag gcgacgaaat gttccgtaca caaatgcgtg cattgcttcg
     4201 tgcatctgtt catggtaatt tacgtatcat gttccctatg gtagctacac tcaaagaatt
     4261 ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa ttgataagtg aaggcaagga
     4321 agtatctgat acgatccaag tcggcatcat gatcgagatt ccagcagcag cagtcttagc
     4381 agacaagttt gcaaaagaag tagatttctt ctctgtaggt acgaacgatt tgattcaata
     4441 cacaatggca gcagaccgta tgaacgaacg cgtttcttac ttgtaccaac catataaccc
     4501 atcaatttta cgtttgatca aaaatgtaat tgatgcagca cacgctgaag gaaaatgggc
     4561 aggtatgtgt ggagaaatgg ctggagatca aacagctgtt ccattgctag taggtatggg
     4621 tctagatgag ttctcaatga gtgcgacatc tattcttaaa acgcgtagtt taatgaaacg
     4681 cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca ttgaaagaat gcgatatgat
     4741 ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa acaacgtatc aaaagctggt
     4801 gtggcttgta aaagagctac gccagttttt ttgttctttt ttaagaaaac gtttttgtca
     4861 gtaagaatag acaaaa
//
LOCUS       DANJLF010000123         4788 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000123 DANJLF010000000
VERSION     DANJLF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            564..989
                     /locus_tag="PWT25_002686"
     CDS             564..989
                     /locus_tag="PWT25_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022696.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="PWT25_002687"
     CDS             1019..1183
                     /locus_tag="PWT25_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022697.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="PWT25_002688"
     CDS             2141..3118
                     /locus_tag="PWT25_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1022698.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="PWT25_002689"
     CDS             3128..3625
                     /locus_tag="PWT25_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022699.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="PWT25_002690"
     CDS             4001..4282
                     /locus_tag="PWT25_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022700.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="PWT25_002691"
     CDS             4284..4700
                     /locus_tag="PWT25_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022701.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          816 c          785 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtatacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DANJLF010000124         4757 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000124 DANJLF010000000
VERSION     DANJLF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            18..1208
                     /locus_tag="PWT25_002692"
     CDS             18..1208
                     /locus_tag="PWT25_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDL1022702.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1342..3531
                     /locus_tag="PWT25_002693"
     CDS             1342..3531
                     /locus_tag="PWT25_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1022703.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3528..>4757
                     /locus_tag="PWT25_002694"
     CDS             3528..>4757
                     /locus_tag="PWT25_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL1022704.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT         1708 a          728 c          861 g         1460 t
ORIGIN      
        1 gaagagagga cttccctatg aacgatttta ctacagaaat tctaaagact ctagcgaaca
       61 aaggcgattt gaatgaatta ttccgtgtcc atttggagaa agcagtcaat acgcttctca
      121 aaacggagtt aacggctttc ctagattacg aaaagtatga tcgcattggt tttaacacgg
      181 gtaattctcg taacggctcc tatgaccgta cggtcaagac cgagtatggg gaacttcatc
      241 tccagattcc gcgcgaccgc aatggtgagt tcaagcaaca gactgttcct gcttatagac
      301 ggacgaatga cacgttagag gagaccgtca ttcacctctt ccgaaaaggt attaccatgt
      361 cggaaatcgc agacttgatt gagaaaatgt atgggcatta ctacacgccc caaaccatgt
      421 ccaatataac aaaatcattt acagaagagg taacggcgtt taatgggcgg gagcttcatg
      481 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      541 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      601 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttggaccttc
      661 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      721 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttccc
      781 gtaatatcag tcataaagtg cgtgtcgatg atcgtaagga agtctgtgat gattttaaaa
      841 tggtgtatca agcctcatct aaagaggtgg cgttggaagc acgtggtact tttgcggaaa
      901 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      961 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
     1021 cctttaataa acaaatcaag aaatacagcc accgcaagga acagttccaa aatgaagagt
     1081 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
     1141 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctaagc caactgattg
     1201 agaattagaa acaatctgca gtagggaaga atcatttaca caaaattatt gacgctccct
     1261 tataatgaga acttttacta acataattta tgataaactt actatataat catattaatt
     1321 aaatagttag gagatctgct tatgtttata tctactcttc aaattcgaaa ttttagaaat
     1381 tatgaatccg aaatctttcg attcgataat gagacaaata ctattatagg tgagaatgat
     1441 tcagggaaaa ccaatgctct tactgcattg agaatcttat tagatgatag ttattactat
     1501 agtagtaaaa cactaaaaga atcagatttt tttcatggaa ttcaaaatgg gtggcaaggg
     1561 cattggatta ttatctctgc gacatttgaa ggaatcagcg aagaagaatt tgacaatgag
     1621 atttgtgctt ccatttcttt aaattctgaa agtcaaacaa tcttagaaga attgatttca
     1681 aatgcagata aaggtgtagg tagtatttct ctatttatta gacctaacaa agctattaga
     1741 aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga atttagaaat
     1801 tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct aaatttctgt
     1861 atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc tagtaatcct
     1921 gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat tttcaagcat
     1981 atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa aaacaatcga
     2041 aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga taatgttgag
     2101 tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc tgaaattagg
     2161 aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt gtattctcct
     2221 aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa atttctttca
     2281 atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct aaatatgatt
     2341 tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact acttaatatt
     2401 atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac tctgttcgaa
     2461 ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc tgtacattta
     2521 gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg caaatctatc
     2581 gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa aaagaacaat
     2641 ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg attgcttttt
     2701 tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc tcaaatagta
     2761 aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa tgttggtagt
     2821 acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag aaagtgtgct
     2881 attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta taaaagcact
     2941 gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa tgaaaacaat
     3001 tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga tgtgaataaa
     3061 gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga taaaaaaaca
     3121 ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa tttagcgatg
     3181 ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg tgaaaaaatt
     3241 gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc atgtcaggaa
     3301 atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt gtacgaggaa
     3361 actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc tgatttgaat
     3421 atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt gtatctaatc
     3481 aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg acaaatatct
     3541 tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa agtgaagata
     3601 acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca aggaaaattg
     3661 cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc acatacacta
     3721 atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat gatgatatta
     3781 atatttgggt ggggacaata catcagtttt gtcttgaatt tattatttat ccttttaaaa
     3841 tgaatctctc aagagtatca aaaggattta caattattga tgattatact caaagagctt
     3901 acgtcagcca aattaataat atgctgaatt taaatctcaa aacgtatgaa ctgaacaaaa
     3961 ttgatttgtc actttctcat gattttaaaa ttaatgaagt tcagtatact caagtttctg
     4021 aaatgtatca cactatgttg gaaaaaaata aagaaattga ttttgatatg attttactag
     4081 tagcgtataa aattttaact gtaaacagta acgttgcacg taatattgct agtaatataa
     4141 gggcgattta tgtggatgaa tttcaagata ctagagaatt acaatacaat gtaatagcta
     4201 aattacttca aaacaaccct caaattcagt caatgtttgt tggagatatt gaccaagcaa
     4261 tttatggatc attagatggt atagctaaat cagttgaaga actagaaatg ctaactagtc
     4321 acacttttca atcaaagacg ctacatggct gttacaggtc taatcagaga ttagtagact
     4381 tctactctaa ttttcagagt tgtccttacg agattgaaag cagagggaat aataaaaacg
     4441 accgtggttt tataagttat gattgcaaag ttactaagga ggatttaccc aacataataa
     4501 aaaatataat aaaggaaaag attgaaagcg gcattcatga aaaagatatt tgtgtacttg
     4561 ctcctcaaca ctatcctctg ttttcactgg gtgagtattt aaagaaagaa ttaccgtatt
     4621 gcaattttga tgctataaat atatcaccaa ttaaggtaaa taatcatagt ttatttttca
     4681 agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga aagctaattg
     4741 caaacgatat tttaatt
//
LOCUS       DANJLF010000125         4720 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000125 DANJLF010000000
VERSION     DANJLF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..1097)
                     /locus_tag="PWT25_002695"
     CDS             complement(<1..1097)
                     /locus_tag="PWT25_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1022705.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="PWT25_002696"
     CDS             complement(1227..2603)
                     /locus_tag="PWT25_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1022706.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="PWT25_002697"
     CDS             complement(2603..3259)
                     /locus_tag="PWT25_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1022707.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="PWT25_002698"
     CDS             complement(3269..4546)
                     /locus_tag="PWT25_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1022708.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="PWT25_002699"
     CDS             complement(4565..>4720)
                     /locus_tag="PWT25_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL1022709.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DANJLF010000126         4710 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000126 DANJLF010000000
VERSION     DANJLF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            106..357
                     /locus_tag="PWT25_002700"
                     /pseudo
     CDS             106..357
                     /locus_tag="PWT25_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="PWT25_002701"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="PWT25_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL1022710.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="PWT25_002702"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="PWT25_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="PWT25_002703"
     CDS             1786..1869
                     /locus_tag="PWT25_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDL1022711.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="PWT25_002704"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="PWT25_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL1022712.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="PWT25_002705"
     CDS             complement(2994..3857)
                     /locus_tag="PWT25_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDL1022713.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="PWT25_002706"
     CDS             complement(3859..4131)
                     /locus_tag="PWT25_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL1022714.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="PWT25_002707"
     CDS             complement(4148..4357)
                     /locus_tag="PWT25_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL1022715.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="PWT25_002708"
     CDS             complement(4455..>4710)
                     /locus_tag="PWT25_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HDL1022716.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DANJLF010000127         4645 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000127 DANJLF010000000
VERSION     DANJLF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(132..716)
                     /locus_tag="PWT25_002709"
     CDS             complement(132..716)
                     /locus_tag="PWT25_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1022717.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(927..1130)
                     /locus_tag="PWT25_002710"
     CDS             complement(927..1130)
                     /locus_tag="PWT25_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022718.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1140..1562)
                     /locus_tag="PWT25_002711"
     CDS             complement(1140..1562)
                     /locus_tag="PWT25_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022719.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1800..2414
                     /locus_tag="PWT25_002712"
     CDS             1800..2414
                     /locus_tag="PWT25_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1022720.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2579..3403)
                     /locus_tag="PWT25_002713"
     CDS             complement(2579..3403)
                     /locus_tag="PWT25_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022721.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3427..3582)
                     /locus_tag="PWT25_002714"
     CDS             complement(3427..3582)
                     /locus_tag="PWT25_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022722.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4041..4298)
                     /locus_tag="PWT25_002715"
     CDS             complement(4041..4298)
                     /locus_tag="PWT25_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL1022723.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4291..4560)
                     /locus_tag="PWT25_002716"
     CDS             complement(4291..4560)
                     /locus_tag="PWT25_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1022724.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1670 t
ORIGIN      
        1 aatattaaca aatgaataaa atccttacaa catttgagaa taagttatca gtagacactt
       61 acaaaaaatt ctcatactat tgtcactttt ctagataaat ggcaaaaatt ttcagcatta
      121 aatgacaaaa actaatgaga atttgtcttt ccatttttct cctccatttt tgccaaataa
      181 cgataaatgg tggttcttga gacgttccac cgttccgcaa tagtcttaat aggtgtaccg
      241 ctatccacta aagtcttcag taattccaaa tcttgttcag acaatttttc aggacgacca
      301 ccaaaccgtc ctcttgcacg agcagctgct cgtcctgctg cggatcgttc caggatcaaa
      361 ttacgctcaa attctgcaaa tgccgcaaac aaatgaaaca tgagctgtcc tgtagaactt
      421 gatttgtcca tcgtaatatt ttcttgaata ctatgaaaac tcacaccacg ctcattcagt
      481 cgattgacga ttgaaatcaa atcttccata tttctaccta aacgatctaa gcgccaaacg
      541 acaagtgtat cgccagagcg aacaaaatca atggccattt ctaatcctgg tcgattactt
      601 tttgccccac tcatatgatc agtaaaaatt ttttcacaac caaatttatt taggctgtct
      661 tcttgtaaat ccaaattttg taaaccagta gatacccggg cgtatcctat tttcaaaaaa
      721 atcactcctt cattaatttt attgtaacat aactttatcc atatagttta actgaacata
      781 gatattgtta tgggtttatg aacatgaaat gaattgtttt tttgaaaaat aaagcaaaaa
      841 aagaaagtgt ccagaaatgg gtgattttat gaacactttc aattagaaaa aaagaatgtc
      901 gactagacac tcttttttta aaatcattat ttttcttttt gatatgtttt ttggtctctt
      961 ttttttcttt tttcttcaga atcttctaga ttagaaattg atttttgaat tttttgtgtc
     1021 tcctgtaaag ttcgctgaac ttgtaaagct gattcttctt tcggtaaatt aattttactt
     1081 agaatatcgc tatcttgata aaaatttggt gtgttcacaa attctggcat aaaaaatccc
     1141 tactttctag ttaaggttcc tgaataagtc gcaatccaat tgtttccagc tgatttataa
     1201 ctttgtaaat acaattttcc atagtaccaa taaccatttc ttttttcttt ccaattccgt
     1261 gattgaggag gtctagaagg ccaaccaact ttatcaaaaa tcatcgttcc tgtcactcgt
     1321 atcgttttag aagctgctag agcaagtaat ggagaaacta ttaaataatt ttcagtaaca
     1381 gttaagtcgt tagttgggtt accaatggct attccgtccc tatctaaagg agttaattct
     1441 tggttattat ctactacagt agttggttca tttgtactat cagcaaatgt aggaattgta
     1501 gttccagtta cactaattcc aatagttaaa atacctaaag ataaaataaa cttatttttc
     1561 attgtctatc acctcgaaat caaaactaag aaaacgtttt cttaataaat cttaacagat
     1621 aaatcaagtt atatactaga aaattcataa aatatatttt taatgtcata atcgtttaaa
     1681 ttttcttgat tgacatttat ttacaaaaaa tattgccatt taaaaagaaa agtgacatat
     1741 ggcttcctcg tgtatgatta gggtgtattt taaatagttg cagaaagtag gtaaaagttt
     1801 tgagagttgc atacatacgt gtctcatcga tcgatcaaaa tgaacaacgc cagattgaag
     1861 aaatgaaaaa attcggtgca gagagaattt tcatcgagaa acaatctggc gcaacgatta
     1921 ctcatcgacc tgttttccaa gaagcattag attacgtccg agatcaagat atttttattg
     1981 tggaagctat tgatcgactt ggaagaaatt atgacgagat catcgcttca gtcaattact
     2041 tgaagaagaa aaatgtgaag ctgatcatta ctagtttacc gatcatggct gaagcaattg
     2101 gcaatcctct cctggataaa ttcattaagg atctgatcat ccaaattttg gccatgatcg
     2161 ctgaacaaga acggactgaa tcgaaacgtc gccaggcaca aggaattaag atcgcaaaag
     2221 caaacggcgt ctacaaaggg cggcccaaat tatacagtgc agacgcaaaa gatccccaac
     2281 gtcgccttgt ttacaaaagc atcgttgaag atcttaaaaa tggtgttgcg attgctaaga
     2341 ttgctaaaga ctacaatgtt acgagacaaa ccgtgtatag aataaaaaaa gacagcatgg
     2401 tcaatgacaa atagttgatc atgctgcttt tattatcttc tcatctttaa tttcctacat
     2461 ataattacta tctataaaat taagtcttac tcagaacaga taaagcataa aattccaaca
     2521 aaccattttt tacttaacta aataaagagt ctgttaatat aatcttcact tatttacgtt
     2581 aatcccctac tgtatttgtt ggttccttat ctatattttc tacaaaatcc ttattatctt
     2641 gtgatttagc ttcgtataat tctttgtatg tcgacacatt ggttatacta attcctatag
     2701 ctagttcttg agactctact tttctctcta gctcctttga caaagtgatt tgagatttat
     2761 ttggttccgt tggagtcaat tctatagatt cttcataaaa atctctatac ataaagctat
     2821 cattaatttt ataaaaaaaa ttaaatgttt ttagcactgc catataacat tcaaaacatt
     2881 cttctatagt tggtttactt ccttgaggag aatgaaccaa tttattacgt ttttctatga
     2941 aatttttaat ttcctttctt atttctttcg gaaactcaat attaaagtac cctttaaaca
     3001 tgctccctaa taatttgtct acagggggag attgtaaatt ctcaaatatt agagttaaat
     3061 caggtttctc ttgttgataa aattccttta cccctatttc acaggctgta attatcgaaa
     3121 taaacctatc tctagtattt gatatatttt tacttctttt aaggagtata agagaaaagg
     3181 ggatactttc gtgtccatgc atgtaattaa ctaattgttg agcttcagaa gttatatctc
     3241 taattatata gttaacggaa ttaggaagtg tctttaactc taatgaaatt tctatccaat
     3301 tactatcttc taatctgtat atgctcgaag tataatttga ttcgtcagca agcatcgcta
     3361 catcccaaac actgatcata aaatctttta aacgttcaat catttcagca atattgctaa
     3421 aatcagctaa tttttcttta gaataaaaaa aacatttata tcagaatcaa taaattctat
     3481 tttttttatt actatatcgt catcattatc ttgaccattt atgtttattg ttccctgagt
     3541 ggggagtact tctttcaaat tcaagttaaa tttatattcc attacttctc ctccaatata
     3601 taaaaattac ttaactgtat ccaaatttaa tatgtaaata aatcattaat ttttagctaa
     3661 attctatgag aagtttattt ttgttgtttt tgctaattaa ctaaaactag tacaaaaaat
     3721 ttaaaataga ttattaaacc tattgcttat tctattcagt catatatata agcgtcatcc
     3781 tttagtaact aacatcccac actacaatag tattataaca tatctattca acttcaaatt
     3841 gttgaaattt aagccattta tatttacttt tcctcaactt tagaaatgat tttactaacc
     3901 ttatatccgt aatacgcgta acaaacacat tcattaataa caatcaacac taagctatat
     3961 tagcttttta aatgactagg gtaaataaaa taaggatcaa aaacttttaa gtttctgacc
     4021 ctttccttac ttccgattgg ttaatagtga tcttttgcag aataaataaa tatcgtttca
     4081 ttttcaactc tatatatcag tctatgttca tctgtaattc ttctggacca ttttccagat
     4141 aaatcatgct ttaatggctc aggttttcct aatccagcaa agggggaacg atcgatatct
     4201 tttattaact tgttaatctt ttttatattg cttttgtttc cttgctcatg ccaataaaga
     4261 taatcatccc aagcatcatc agaccaagcc ttaatcatca gattcaacct cgattaagtc
     4321 atgtgtttta aatgcacctt tggagaattg ttcatctcct cgacgaattt tttccatgac
     4381 gtaattatta gaaagtgttc tcaacgtttc ttgcatagaa tcataatctc tttttgataa
     4441 tacaacaact gtatcttcta catctttact tgttacaata agtgtttcag catcctcatt
     4501 aacttgtttc atataactac gtaaattttg gcggaaattt gaataagcta ctgcttccat
     4561 tcctttcacc ccttatttat ttctataaaa caattgtaca ttatattgta caattaagca
     4621 aatgattaat attctaactt cttat
//
LOCUS       DANJLF010000128         4610 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000128 DANJLF010000000
VERSION     DANJLF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(85..339)
                     /locus_tag="PWT25_002717"
     CDS             complement(85..339)
                     /locus_tag="PWT25_002717"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL1022725.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="PWT25_002718"
     CDS             complement(450..704)
                     /locus_tag="PWT25_002718"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1022726.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="PWT25_002719"
     CDS             complement(897..1172)
                     /locus_tag="PWT25_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL1022727.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="PWT25_002720"
     CDS             complement(1144..2097)
                     /locus_tag="PWT25_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1022728.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2709..4202
                     /locus_tag="PWT25_002721"
     CDS             2709..4202
                     /locus_tag="PWT25_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL1022729.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4337..>4610
                     /locus_tag="PWT25_002722"
     CDS             4337..>4610
                     /locus_tag="PWT25_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL1022730.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1542 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2161 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2221 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2281 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2341 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2401 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2461 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2521 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2581 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2641 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2701 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2761 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2821 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2881 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2941 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     3001 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     3061 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3121 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3181 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3241 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3301 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3361 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3421 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3481 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3541 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3601 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3661 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3721 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3781 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3841 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3901 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3961 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     4021 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     4081 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4141 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4201 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4261 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4321 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4381 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4441 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4501 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4561 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa
//
LOCUS       DANJLF010000129         4472 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000129 DANJLF010000000
VERSION     DANJLF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            320..1051
                     /locus_tag="PWT25_002723"
     CDS             320..1051
                     /locus_tag="PWT25_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL1022731.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1196..3223
                     /locus_tag="PWT25_002724"
     CDS             1196..3223
                     /locus_tag="PWT25_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL1022732.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3331..4428
                     /locus_tag="PWT25_002725"
     CDS             3331..4428
                     /locus_tag="PWT25_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL1022733.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1571 a          717 c          923 g         1261 t
ORIGIN      
        1 aaatataata tacatttttt atttaattat aattaatatt gattgaagtc taggcagtca
       61 agtcacattt tgatagatag gagggggact tacgaattat tttaaaggct atgacagctg
      121 tatttctaga ttgtgtttgc atcaagttgc aataaaaact agaaatcaaa tcgggctgat
      181 aatcatttat aactaatcga tacataggac caaatatagg tctatgttct tttatttcat
      241 aagtatcgta aaacagaatt ttgctatgga ttaatccaat cgactgatat gtttagtaaa
      301 taaagaaaga ggaatgtcaa tgatgaaaaa agaaatcgta ttaacagcag caatgatgtt
      361 attcagcatg gtagcaagta cgacttttgt ttctgcaact gaagtatacc caaaggaata
      421 taacacagaa ggaacgatta catttgaagc aggagatgag ggtgtaactc ctcctgttga
      481 tccagagaat cctgatccca ataaacctgt cgatccatca gatcctccaa gccctggaac
      541 aggaggggca ttatcgattg attatggatc aaaattcaaa tttgggactc aaaaaatttc
      601 tactgcggat aaaacgtatt atgcagctgc tgatgtgatg aacgatgggt ctagaaaacc
      661 gacatatgtc caagtgacag ataggagaag taccttatct gggtggaaat taagtgtctc
      721 tcaaccagaa cagtttaaaa ctgccagtgg ggatgaacta gttggagcac agctgaaatt
      781 tacgaaaggg caggctgttt ctttggttga tccaacatat acgccgcaaa cggtgaattc
      841 tgaattaacg ttgacaccgg gaggaaacaa tacgttagca attaatgcta aatcaggaac
      901 aggtgtgggg acttgggtct atcgatttgg tgcaaatgaa aatgaaaatc aagatgcggt
      961 acagctttcg gtacccggta aatctgtcaa gttagcgcaa caatattcca ctaaattagt
     1021 ttggacatta gaagatacac ctaacaacta gtttactgaa aagaactgtt aaaaataaat
     1081 acgtagtcat attgtcttgc gaggtaatcg agcaaaaatt gtcaaaggct aggctaaatt
     1141 tgtttggata cgaatagttc atctaggaat ttaacgatca ggaggaaaag atatattgaa
     1201 aaagaggagg atcgtgctca ttttaagcag tctcttagtg actaatatct ttccgatcat
     1261 agggtactct ttggaattga atcagttgga gcatggaaat agaagagacg aagaaagcaa
     1321 actcacttcg acattcgaca gtttaataga atccaaccag acacaagaaa gcagtgagga
     1381 tttaagtatt tcgagtagtg gagagaccac tgacaacgta gaagctgagg gttcggacaa
     1441 acaaggacta gaagatgcac caatttctgc ggttgagctg cctgacgatc ccaatattat
     1501 ttctatcgat aaaatttttc aaacacctat cggtactagc acgtccatat tggaaaatgg
     1561 gaagttactg cagttgaacc cagcagaaag gtctcaaaga ggagctattt ggtcaaataa
     1621 gaagattagt ttactctcgg attttacatt taaatcttat ttatacttag gaaatcaacg
     1681 agatagagcg ggagatggca tgacctttac attgactggc gatcctagaa tggctagtaa
     1741 tcccagtcaa gttattggat cgccaggtat gggaattggc tcatattcta ctcgttctgg
     1801 ccaaccttac attgcgaatg cgctctcgat tgagtttgat acgtataaaa atagtggttc
     1861 ttctgatcga atggatcgag agataactgc tgataataga gggcatggac atgtcgcttt
     1921 tgtcacgcct aaaccaaata ataataatta ttctggagag catactggcg tgacggtagc
     1981 tcctacattt ctttctaatg gcacatggcg cttgttaata attaaatggg acgcggagca
     2041 aagaagtctt acttatgatt tggaaggtgt cggtcactct tctcggatta tacctgatct
     2101 cattaggcag tttggttcta cagaagtaaa ttgggggttt acttcttcaa caggtacttt
     2161 ttttcaagaa aatgctgtcg ctgtcacgcg acttccatcg aatgtcacat caacagctga
     2221 tgtaaaagtc aacgatagtg attatggggc catcgctgaa gctggaggaa acgataagat
     2281 aactatcaga aatacattga aggtcgaggg ggattttctt gaatataaga aaatgcagac
     2341 tagtatcgac ctcccacaag aattaactat tcccgagcat atcttgatca atggaaatca
     2401 aattgatgcc aaagatatct tgataaccgg aaatcgagtc acgctaaatc tgacagatta
     2461 ttttattgct ggagatacgt taataatcga attggagaca actttgcagg aagtaattcc
     2521 tgaaaaagta ctcactatgc attttgaata ccttgaaaac gggaatttga ttgagcaatc
     2581 gaatgaaata aacctaaaga tcgcaaagct gaaggagata gatatccatg tttattataa
     2641 agataaagac agtcagattg atattgctga aatgaaaaca atcactggga aaataggaga
     2701 gaagtataaa gaaactccta ttgatattga agggtatgtt tttgactcgg attcagcgaa
     2761 tacagaaggc atcttctccg aaaatacaga ggatatctat ttttattaca gaaaaggcaa
     2821 actttatctc tcaactgctc ctcaatatgt caattttgga aaacataaaa tatccagtag
     2881 accattaagt gaattcggac agttttcagg tgggttgaaa attatagatg aacgggcttt
     2941 tggaagttgg aaattacaat tgaaacaaga gaaagttctt acaaatggag attttgagct
     3001 tccagaagct ctttcctttg ttacctctga aaatacaacg gttataggga catccgctgt
     3061 tacgattttc gaaagtgatc aaaagggtga aagcgatttg tcctctcttt tagattctac
     3121 agctcatcgt ggaatcagaa tagatattcc tgtagaaaac caaagaatcg gaacctttga
     3181 ggggaaacta tcttggatac ttgcagatgt cccaggaaat taacaggaag agaagaatat
     3241 aacaaaatta ttgagtttca agaagggcac aggtaccata ggctcttgtg tcccacagag
     3301 acaagatttt gttatagtct tctcttacat atgattaaag gagcgatttt cttgagacca
     3361 aaacaacaat tatttataac actcctagtt attttagggg cactttttaa caatatatca
     3421 gtgtcagcta gtgcatttaa tttttcagtg acgccgataa cttcagagaa tcaaatcgac
     3481 aagagaaaaa cttacttcga tcttcaactt gatccggatc aggaagtaga agtaaaagcg
     3541 gaattgagaa atgatacaga aaaagaagtg aaaatcgata tttctgtgaa tagcgcaacc
     3601 accaattcaa atgtgatggt tgaatacgga aaaaacgaga ttgaaaaaga catgagcctg
     3661 atctttgatc tggtagatta tgtgagctat ccacagatag tgacgctaaa acctaaaagt
     3721 gtccaaaccg ttgcttttca tgtcaggatg ccgaatgagc gatttgatgg tgtattggct
     3781 ggtgggatca cattccaaga aatccagacg gaaaaggacc aaacagaaac taaggatcaa
     3841 agcttatcga ttgagaatga atacacctat attattgctt tgttgatgca acagacactg
     3901 aacgaagttg ctccgaacct tactcttcat gaagttaaac cagatcaaat caatgcaaga
     3961 aatgtcatct tagcaaacgt tcaaaatgat caaaaaacct atatcaatca agtagttatt
     4021 gaaacaaaaa ttacaaaaaa aggacattct gaagttttat atcaagagga aaaagaagga
     4081 ttgcagattg cgccgaatac aaatttttct tttccaactg ctttaaatgg tcaaccgcta
     4141 accccaggtg aatatcacct tacaatgact gttttaggaa atgaaaatga aaatggaaaa
     4201 ttttcgagaa aaaaaggaaa tactacgatc aactatacca atcaatgggt atttgaaaaa
     4261 gattttacaa tagacggaaa agtagccaag gaattgaaca cgacggatgt tacgattaaa
     4321 aaagacaata attggattta tatcttagta gcttcacttt tcttattgtt tattttatta
     4381 tgcatacttt tgtttgtttt gagaaaaaaa gaagagaaag aaaagtaagg ttaagataac
     4441 aaatagtaaa tttttattat tgtaggatgg tt
//
LOCUS       DANJLF010000130         4425 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000130 DANJLF010000000
VERSION     DANJLF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            690..1658
                     /locus_tag="PWT25_002726"
     CDS             690..1658
                     /locus_tag="PWT25_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1022734.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="PWT25_002727"
     CDS             1669..2484
                     /locus_tag="PWT25_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL1022735.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="PWT25_002728"
     CDS             2552..3262
                     /locus_tag="PWT25_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1022736.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="PWT25_002729"
     CDS             3298..>4425
                     /locus_tag="PWT25_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL1022737.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DANJLF010000131         4324 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000131 DANJLF010000000
VERSION     DANJLF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..281)
                     /locus_tag="PWT25_002730"
     CDS             complement(<1..281)
                     /locus_tag="PWT25_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL1022738.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="PWT25_002731"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="PWT25_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="PWT25_002732"
     CDS             complement(2359..2721)
                     /locus_tag="PWT25_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022739.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="PWT25_002733"
     CDS             complement(2725..3063)
                     /locus_tag="PWT25_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022740.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="PWT25_002734"
     CDS             complement(3042..3461)
                     /locus_tag="PWT25_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022741.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="PWT25_002735"
     CDS             complement(3454..3687)
                     /locus_tag="PWT25_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022742.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="PWT25_002736"
     CDS             complement(3684..>4324)
                     /locus_tag="PWT25_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL1022743.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DANJLF010000132         4310 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000132 DANJLF010000000
VERSION     DANJLF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(65..1285)
                     /locus_tag="PWT25_002737"
     CDS             complement(65..1285)
                     /locus_tag="PWT25_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL1022744.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="PWT25_002738"
     CDS             complement(1305..1916)
                     /locus_tag="PWT25_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1022745.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="PWT25_002739"
     CDS             complement(1959..2891)
                     /locus_tag="PWT25_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL1022746.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="PWT25_002740"
     CDS             complement(3312..4172)
                     /locus_tag="PWT25_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1022747.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DANJLF010000133         4055 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000133 DANJLF010000000
VERSION     DANJLF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(164..1669)
                     /locus_tag="PWT25_002741"
     CDS             complement(164..1669)
                     /locus_tag="PWT25_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1022748.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1666..2352)
                     /locus_tag="PWT25_002742"
     CDS             complement(1666..2352)
                     /locus_tag="PWT25_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1022749.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2545..3966)
                     /gene="gndA"
                     /locus_tag="PWT25_002743"
     CDS             complement(2545..3966)
                     /gene="gndA"
                     /locus_tag="PWT25_002743"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL1022750.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1115 a          857 c          693 g         1390 t
ORIGIN      
        1 gttttttcgt tcatctaaaa gatacttaca tttaattaac ggatgttatt ctatttaatt
       61 gttcgctagt ttttttataa tgacatccct gaatttctca cttgcttgta ttttgtacct
      121 tattctttgt tggatatata aaaaaacaag taaagcacat gctttacttg tcttctgcct
      181 ttgcgatagg aataaagatt ctaaaaattg tcccttgccc tactacactt tctgctaaga
      241 tacgtccttt atagttttca accagttgtt tagcgataga caaccccaga ccatttccac
      301 ccttcgttct agctcttgct ttatccactc gataaaaacg attgaagatc ttattaaggt
      361 cttctttggg tattccttcg ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg
      421 aagaaataga gatatggact tcttttcgtg tagttgaata ctttactgcg ttatccaata
      481 tgataatgat taattgttca aagtgattac gataaatttg cagggtcact tcttttgtca
      541 aatcatcatc taaagtgatc acatagtctg gatataatat cttgaaatta ttataaactt
      601 gataggtgac ttcttttcct ttagttgttt cattcgcata atgaacatct acttgttctg
      661 ctcttgataa gtctagcatc tcttgaacta agcttttcat acggctgatt tcttgcatgc
      721 tggcgcttaa tgattcttct aatatctcag gatcatcttt cccccaacga tttagcagac
      781 taagatgccc ttcaataatt gcaacaggtg tccttaactc atgagatacg tcttccacga
      841 attgctcttg ttgttcaata tacaaacgca tacggtcaag catctcattg aatatttcag
      901 ctaaatctgc caattcatca tttgtgtcga tttcaggcat gtggatatct gattgcggat
      961 ctttacgtat agtatccatt gtatctctga gtaccttgag tggttttaag aaataagaag
     1021 ataaaaagaa acctaaaata ctacttaaga tcaaagaaac aacttctaaa acaataagag
     1081 tcaataatag cttatttcga atatcataga aagaagataa ttcatagaag ctttgcgcat
     1141 agccgatctt ttctcttgta cctttagaat atataggctg gatagataaa aagccagttt
     1201 ttccttctag tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg tttcgactgt
     1261 tttcatgggt tttaaaaatc aagttctcgt ccaaatcata aacataaaga tccattgatg
     1321 gctgccctag ctcagctata aaggaatcga tcttcattaa gtttccttcg accagtgaag
     1381 tatgatctac gacctcatct ccgtcatttg tcgctttcgt cagattacga tacgtattta
     1441 ctaatgtcag ttcttcatcc gagttagcca aacgagaagc tacttctgaa atcgtacgct
     1501 ctacattgtt tctttctttt gccacaatca gattgataga tgatttatag gtaatgacag
     1561 cgaaaattgt aaacacaacg aatatgaaga aagaactcgc aaaggcccac ttgattgtca
     1621 aggagggccc tttcagttct cttttttccg attttttcgg ctttatcacg agcgcatcac
     1681 ataacctgtt ccgcgtacag tttggatata gctttcctcc ccgggaacgt caattttgtt
     1741 gcgaagataa cgaatatata cgtccactac gtttgtttca acttctgttt cgtatcccca
     1801 cactttattt agtaaaacgt cacgtgctaa taccacgttc acgttttcca ttaaagtaag
     1861 cagcaactcg tattctcttt ttgttagttc aattacttca tttccgcgac gtacgacacg
     1921 gttttctttt tcgatagtca aatcgcgata agtaatagta gtttgtttag ccacattttt
     1981 atcgccttca atatcgattc gacgcaacaa tgcacgaaga cgtgctaata attcttcgat
     2041 agcaaatggt ttgacgatgt agtcatctgc tccgtggtca agtccagata cacgatcgat
     2101 cacagaatct cttgcagtca tcataatgat tggtgtattt ttgacttggc gtacacgtct
     2161 gcagacttct aaaccgttca attctgggag catcagatcc aacaatatgg catcccactc
     2221 attatttaat gccgcatcta aaccagttcg gccgttgtag tggacttctg cgttataccc
     2281 ttcatgcttc aactctagct cgacaaatcg tgccaagttc ttttcatctt caatgattag
     2341 aatattgctc atttattatt ttatcctttc tattctactg gctttttaaa aactctatca
     2401 ttataccgat aatatgctta aaaatctata gtcattttat catggatagc aattttaaaa
     2461 aagagctgaa gtctactcat aacaatcgac ttcagctctt tcgtcctctt tttattttct
     2521 catttcctaa cagccgaaag actattattc ttcatggtac catgagtaat ggaagattcc
     2581 ttctttgtcg acacgctcat aggtatgtgc gccaaagtaa tcgcgttggg cttggattat
     2641 atttgcaggc agacgttctg ctctgtatga atcgaagtaa gcgatggctg atgagaaagt
     2701 cggtacaggt acgccagctt ggacagccaa ggcaacgacg tctctgactg attgctgata
     2761 ttttttcgtg atttcaacga agtaatcatc tagcaataaa ttttcgatct caggattttt
     2821 gtcataggca tccgtaattt tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca
     2881 gatttttgcg atttcaccaa atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa
     2941 ttgagcaaat ccttgggcat aactcatgat tttactgaaa tacaacgctt ctcggatttt
     3001 ttcgaccaat tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag acaaaatctt
     3061 gcttgcttgt acgcgttctt ctttataagc agaaatatag cgggcaaata cagattcagt
     3121 gatcaatgga agtggcacac ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc
     3181 tttattccca gctgcatcaa ggatcacatc tactactggt ttacctgttc cttcatcgtc
     3241 tttacgtgtc agaatgtcag cagtgatttc tatcaaatag ctgtctaatt cgccttgatt
     3301 ccattcttta aagatatcag ccatttcttc tactgaaagg tttaaaatat tcttcattaa
     3361 gtcataagat tcagcaatca gttgcatatc tccatactca ataccgttgt gtaccatttt
     3421 cacataatgt ccggctccat ttgggccaat atatgtgaca catggtgctc cgtcttctgc
     3481 ttttgcagaa atttgctcta agattggtgc aactagcgca taggcttctt tttgtccacc
     3541 aggcatgatt gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat
     3601 aaagttgatg ccagagttgg ctaactcttc attacgacgc atcgtatctt taaagaatgt
     3661 atttcctccg tcaatcaaaa tatcgccttt atcaaggtga ggaagtaact cttggattgt
     3721 tgcatctgtt gccggtcctg ctttgaccat tagcaagatc cgacgtggtt tctcgataga
     3781 ttctacaaat tcttcgattg tatatgttgc tttgaaattt ttatctgggt gttcttctac
     3841 tacagcggtt gttttagccc ctgtacgatt gaataaagcc actgaatagc cgcggctttc
     3901 tatgttcaag gctagatttt tacccataac ggccataccg acaacgccga tttgttgttt
     3961 tgacatatga actcctccta catacgggat ttctccctaa tctcttacat tatagcttaa
     4021 cacggctaca ttgcaaacta aagaacaaca tattt
//
LOCUS       DANJLF010000134         4051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000134 DANJLF010000000
VERSION     DANJLF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(74..616)
                     /locus_tag="PWT25_002744"
     CDS             complement(74..616)
                     /locus_tag="PWT25_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL1022751.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            751..1140
                     /locus_tag="PWT25_002745"
     CDS             751..1140
                     /locus_tag="PWT25_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022752.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1144..1908
                     /locus_tag="PWT25_002746"
     CDS             1144..1908
                     /locus_tag="PWT25_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1022753.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2037..2984
                     /locus_tag="PWT25_002747"
     CDS             2037..2984
                     /locus_tag="PWT25_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1022754.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3114..3854
                     /locus_tag="PWT25_002748"
     CDS             3114..3854
                     /locus_tag="PWT25_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022755.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1352 a          688 c          815 g         1196 t
ORIGIN      
        1 tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt
       61 ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg
      121 aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc
      181 gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt
      241 ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca
      301 aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg
      361 ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga
      421 caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa
      481 tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca
      541 tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca
      601 tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa
      661 tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat
      721 agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc
      781 agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca
      841 attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt
      901 tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat
      961 ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc
     1021 ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag
     1081 acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa
     1141 caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt
     1201 ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca
     1261 agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc
     1321 ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat
     1381 acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta
     1441 gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg
     1501 acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt
     1561 tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat
     1621 gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg
     1681 cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa
     1741 atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa
     1801 gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa
     1861 gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa
     1921 atcactcatg acgatagagg attttccgtt ttttattttg ggctataata aactctgtta
     1981 ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc
     2041 ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca
     2101 tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg
     2161 aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca
     2221 tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga
     2281 tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg
     2341 ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg
     2401 attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg
     2461 attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa
     2521 gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa
     2581 gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt
     2641 ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag
     2701 ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta
     2761 taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt
     2821 tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg
     2881 aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc
     2941 gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata
     3001 tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca
     3061 atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag
     3121 aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa
     3181 ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc
     3241 agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc
     3301 gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa
     3361 agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag
     3421 gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag
     3481 agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt
     3541 ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg
     3601 aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag
     3661 aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa
     3721 caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta
     3781 tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata
     3841 gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg
     3901 cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca aatttttttg
     3961 agtttaagcg aattttttct cccaataagt aagaaaataa tggcaaaata aaaaaacaaa
     4021 ctattttcgc agatcgtttg caaaaatagt t
//
LOCUS       DANJLF010000135         4036 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000135 DANJLF010000000
VERSION     DANJLF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(242..391)
                     /locus_tag="PWT25_002749"
     CDS             complement(242..391)
                     /locus_tag="PWT25_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022756.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="PWT25_002750"
     CDS             853..1065
                     /locus_tag="PWT25_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022757.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="PWT25_002751"
     CDS             complement(1343..3142)
                     /locus_tag="PWT25_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL1022758.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="PWT25_002752"
     CDS             complement(3266..3862)
                     /locus_tag="PWT25_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL1022759.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DANJLF010000136         3924 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000136 DANJLF010000000
VERSION     DANJLF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..1449
                     /locus_tag="PWT25_002753"
     CDS             <1..1449
                     /locus_tag="PWT25_002753"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL1022760.1"
                     /translation="ADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIA
                     PEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRN
                     VTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQI
                     VTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLIL
                     HGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGK
                     LAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIG
                     MPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYA
                     ERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVL
                     EKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1439..2656
                     /locus_tag="PWT25_002754"
     CDS             1439..2656
                     /locus_tag="PWT25_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1022761.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2669..>3924
                     /locus_tag="PWT25_002755"
     CDS             2669..>3924
                     /locus_tag="PWT25_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL1022762.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1359 a          672 c          818 g         1075 t
ORIGIN      
        1 gcagatgaat ccttagagga atatgacaca gtaagcaaac aaacaatgtt gtatcgtgaa
       61 ttactttcag atgaagaatc aaaagaagat ctgattgcca caatcgttga tattttggga
      121 tatgcaattg caccagagta tcttttcaac gtcttagcgg atcaagcaaa acaagcaacg
      181 ttccaattga acgatctaaa caaagccttt gtgcaattgg catctaccta caatcaattt
      241 aatgggttat ttgatgatgt ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa
      301 cgaaatgtga cgattaccga agtcatcaag aaattgaatg atgtagatgt tttagaacac
      361 gatggggacg tgatcgggga tgcgtatgag tttctgatca gtcaatttgc ttcagaagca
      421 gggaagaaag ccggagaatt ttacacacct catatggttt ctgatatgat ggcgcaaatc
      481 gtaacgctcg atcaaaagga acgacgattc tttagtgttt ttgatccaac gatgggttca
      541 ggttcattga tgttaaacgt acgtaactac ttaacacacc cagataacgt taaatatcat
      601 gggcaagaac taaatacaac gacatacaat ctagcaaaaa tgaacttgat tttacatggt
      661 gtcgacgccg aagaaatgaa tctccgcaat ggggatacgt tgaataagga ctggccaaca
      721 gatgaacctt acacatttga tgcagtagta atgaacccac cttattcggc taattggtca
      781 gcagatacga ctttcttaga tgattctcgg tttaatcgtt atgggaaatt agcgccaaaa
      841 tccaaagcag attttgcgtt tcttcttcat gggttctatc atttgaagga aacaggtacc
      901 atggccatcg tcttaccaca cggagtttta ttccgtggcg ctgcagaagg ggtcatccgt
      961 caaaaattac tagaagatgg cagtatttat gctgtgatcg gtatgcctgc gaacttgttc
     1021 tttgggacat cgattccaac gacggtgatt gtgttgaaga agaatcgtca aactcgagat
     1081 gtattattca ttgatgctag tcgtgaattt gttaaaggga aaaatcaaaa taaactatcg
     1141 gaagaaaaca ttcaaaaaat cctcgaaacc tacgctgaaa gaaaagatgt cgagaaatac
     1201 gctcatttgg caacctttga tgaaatcaaa gaaaatgact ataacttaaa tatcccacgc
     1261 tatgtagata ctttcgaaga agaagaacca attgatatgg ttcatgtcgg aaatgacatc
     1321 aagaagatac gacaagaaca gcaagtgcta gaaaaaaaac ttttagaagc tatctcttcc
     1381 ctgcaaacaa cacctgaaaa tgaagcgtgg ttacaaggtg cgctggaggt gttcaaacat
     1441 gagcaatgat acacaaccag aaatccgttt cccaggattt acagatgatt gggaacagag
     1501 gaagttgggg gacttcgttg tagattatgt tgaaaagaca agtgtacaaa atcaatttcc
     1561 tatgctaact tcatctcaac aaaaagggat agttttgcaa gaagactatt ttgcaaatcg
     1621 gcaagtaact acagaaaata atattggtta ctttgtacta cctagaggat atttcacgtt
     1681 ccgtagtcga agtgataatg atgtgtttgt gtttaaccgt aatgacatta ttgatagagg
     1741 gattataagc tatttttatc ctgtatttac tttaaaatct gctgattccg acttcttctt
     1801 aagacgtatt aacaatggaa tccaacgaca attatctatt caagcagagg gaacaggtca
     1861 acatgttctg tctctaaaaa agtttaaaaa tatagtagca atgtttccta gtgaggaaga
     1921 acaacaaaaa atcggcacct tcttcaaaca acttgacgac acgatcgctc ttcatcagcg
     1981 taagttagat ttgctgaagg aaacgaaaaa aggattctta caaaaaatgt tccctgaaaa
     2041 cggagcaaaa gtgccagaaa tccggtttcc tggttttacg gaagattggg aagagcgtaa
     2101 gttgggagaa ttaggtaaaa ctcaatcggg aattggattt cctgatgccg agcaaggtgg
     2161 ttctgaaggt actccatttt ttaaagtttc cgacatgaat aacattggta atgagtatga
     2221 aatgagaaat gcaaatcatt atgtatctaa tgagcaaatt gagcgtaaaa aatggaagcc
     2281 aataaaagat gtgccagcga ttatatttgc taaagttggc gcagcaataa tgcttaatag
     2341 aaaacgtttg gtcacaagcc ctttcttaat agataacaat accatggctt atttatttaa
     2401 taatacttgg gatatctatt ttggtaaaat attgtttgaa actataaatt tgccaagata
     2461 ctcccaagta ggggcattgc cttcttataa cagttctgat attgagaata tcagtgttaa
     2521 ggttcctgta aaggatgaac aacaaaaaat cggtactttc ttcaaacagc tagatgatac
     2581 gatcgctctt catcaacgta agttagattt actaaaagaa acgaaaaaaa gcttcttaca
     2641 aaagatgttt gtctaggagg gaagaagaat gaaaattagt caccgtgagg aagcagaagt
     2701 cgaagaacag ttgattcgtg tacttggaga agggcataat cagtggacgt atcgtccgga
     2761 tctaaaatca gaagaagacc tttgggtcaa tttacgccaa aaaattatct caaataatca
     2821 agcagaactg aatgattttc ctttgaccga taaagaattt gaaacgatca aaacggaatt
     2881 actgctgcga acgaaaaccc catttgatgc tgcgaaatgg ttaaaaggtg aaaatgggat
     2941 ggctcggatc acgattgaac gggaagattc gcaattgggt tctgtctcac ttatccttta
     3001 ttccaatcaa gacattggtg gaggaatctc tacttatgaa gtggtccatc aaattgcaaa
     3061 acgtggaagt aacatagaag cacgggatcg acgttttgac gtcaccttat taatcaatgg
     3121 attaccgatt gtccaaatcg agctgaaaca agtaactgca aaagatggtg tttatcaggc
     3181 atttaaccaa atcaaaaaat atgcagaaga aggtatgttt cggaacaata tcttttcgac
     3241 cttacagttg tttgtggttt ccaatgaaca aaccactcgg tattttgcaa atgccttacc
     3301 gaaagacttg caccctaagt ttttatttag ttggcgaacg aaagacaatg aaaaagtaga
     3361 aaacttatat gagttttgta aacaagtgtt aaatatcccg gatgctcatc gtttgattgc
     3421 ggattataca atcgtcagtg aagatcaaga caataaaacc ctcatggtgt tacatcctta
     3481 tcaagtacat gcgattcaag ccttgtttat tgccgcaaat aaacatcaat cagggtatgt
     3541 gtggcatgcg actggttcag gaaagacgtt gactagtttt gtatctacta agcttttagc
     3601 acgaaaatcg gggatcgatc gaacgatcat gttagtggac cgcaaagatt tagataatca
     3661 aaccaccaca gagtttacaa aatttgcttc ggaatttaat actgggattt cttccgggaa
     3721 cgccaaagcg aatagcttga tcgttggaac gggaagtgcc aaagaattaa gtgaaaccct
     3781 gttggcagat gccaatgcaa atgttgtaat cattacaacc cgtcaaaaac tagatgcggc
     3841 gttgaaatat gcaaagaaac aagaagagaa aaaaggaacc aatcgcttcc aaaaattaat
     3901 gggccaacat atcgtgtttg tagt
//
LOCUS       DANJLF010000137         3894 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000137 DANJLF010000000
VERSION     DANJLF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..115)
                     /locus_tag="PWT25_002756"
     CDS             complement(<1..115)
                     /locus_tag="PWT25_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL1022763.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="PWT25_002757"
     CDS             246..698
                     /locus_tag="PWT25_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022764.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="PWT25_002758"
     CDS             712..1401
                     /locus_tag="PWT25_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022765.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..3877
                     /locus_tag="PWT25_002759"
     CDS             1526..3877
                     /locus_tag="PWT25_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL1022766.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1320 a          519 c          795 g         1260 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3841 tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta ttta
//
LOCUS       DANJLF010000138         3793 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000138 DANJLF010000000
VERSION     DANJLF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            350..1408
                     /locus_tag="PWT25_002760"
     CDS             350..1408
                     /locus_tag="PWT25_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL1022767.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
     gene            1410..2480
                     /locus_tag="PWT25_002761"
     CDS             1410..2480
                     /locus_tag="PWT25_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022768.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            2467..>3793
                     /locus_tag="PWT25_002762"
     CDS             2467..>3793
                     /locus_tag="PWT25_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL1022769.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQM"
BASE COUNT         1268 a          461 c          596 g         1468 t
ORIGIN      
        1 tttagagaag agtttattaa gtcgagatgc gtttcgagta ataaatttaa tcatttgtct
       61 gctttatttt atgcctagta cttttaatgt aactaatatc ccgatgaaaa tagcttttgc
      121 ttggggtgga attatagttt tgtatgaggt ttttataaag aaaaattttt ttaaaatgcg
      181 tttttcttgg ctactgattg gagcaatgat tagttttatc ttaactataa tcattaacag
      241 agaaaatttt ttaattccat ctatttataa ttttggatat ttgctgatta ctttgatagt
      301 aatttttcct gcagactttt cacaaagtga agaggaaaaa aagaaaaaaa tggtgtattt
      361 taataatgtt ttcattatca taattttttt agctgcgcta atatctatct atcaatttat
      421 ttttttgatt tcttatcatg tcccaacagg aacgccagga ttacttgcaa gacaaggttt
      481 tattgaacat cggttatttg gtgtctatac aagtccaaat gttggagcta tgtttggata
      541 tacttctatt attttaagtt taattagtgt attaatttca aaaataacaa aaacgattcg
      601 tgttttttat atgatgaatt ttatcgtaca aatattttat attacacttt cttcgtctag
      661 aggtgcccag gtagtaattt gttctttttt agtattattt cttttgttgt ttgccaattc
      721 aaaatttaga aagagcatta gagaagtact acctgtaaaa atcaaatgga tggtaagcat
      781 attattagtt ctattggtat atttttcttt tggtactaat tatgctaagg attttttagc
      841 aacaattcct gtatcgattg atagaaaagt agaagttata gagtcaaatg aatcagataa
      901 aactccactt ccagaaaaag aagtaatatt acagcattct agtgataact ccgaaatttc
      961 agctggtcgt tttacgattt ggaaagctgc gcttaaagtt ataagaccaa ctttgttatt
     1021 tggttatgga gagaccgatt tctatgggaa tagcgatgca aaatttttga aaaaagtaga
     1081 tcttgatact acagatgtga atgaactaaa aagagcacat ggaaacatgc ataatggatt
     1141 tcttcaagta cttgtatcaa gtggtataat tgcttttata tgtatctatg ctttctatgt
     1201 gttaaatatc tttagtttac tcaaaacatt ttttagaagt aaagtgaatt atgcgtttct
     1261 tggtattgtt ttaattttta tactatctat ttttatagat gaaatggtgg aggctcatgt
     1321 acttttcaat aagcgcgatg tcatttcaat tgttttctgg tattatttgg gaagcatttc
     1381 ttacttgtat ttaaaaaagg agaattaata tgaagacagc tttcattgct tggactcctt
     1441 tacatttgat taatatatta aatgtttatc aaaattttta ccgagaatca aaaggagatt
     1501 tatttattta ttctgaattt gaaaacgcta gaagatatag tgagcgtata aaagaattag
     1561 ggctatttaa tgatgttgtt tttgtgaatc accggaaaat cggttcttcc tttaaacgta
     1621 aaattaactt gttaactaac cataatcaat ttttctcgga tgatttatcc gtttatgatg
     1681 aaatttttat acagggggaa aattatttca caaaaatact tttctccaat gtttacaaaa
     1741 aaaatccaga tgtaaaacta aattatatag aagatggttt gggtacttat ttgaagaaga
     1801 gtatattttc aacaagtaaa aacgttcaaa gagtttttaa aatattaaat aaaaatagca
     1861 tcttcaaaaa aaaaattgac gattattttg tctattgtcc ggaattgtta ctttatgatg
     1921 taaaaaatat aaaaaaaatt cccaaaatag atgaaaacag taaattgatg aaaaacatac
     1981 aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac
     2041 ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta
     2101 tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt
     2161 atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg
     2221 acttctcttc aacacttttg attagtccta tatctacaat ttctttttca ccatctatga
     2281 tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat gaatatacag
     2341 cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt gaacattata
     2401 accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta agaaagttgg
     2461 tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc cagtttctgg
     2521 tatactatag ggaatttttt tattaaggga atttcattca taactattcc tatttttgta
     2581 aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt tgtatcaatt
     2641 ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag ttttgactat
     2701 aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact ttcgttaatt
     2761 gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt tgatatgaac
     2821 caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat tagttatatc
     2881 cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc ttttatatct
     2941 acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt tgatgataaa
     3001 tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt agtaatttat
     3061 ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag atacgcttta
     3121 aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc acaattcgat
     3181 agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc ctacattagt
     3241 aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg ggttccatgg
     3301 ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag taactattat
     3361 ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga tttagcaaaa
     3421 tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat aaccttaggc
     3481 tatttctttc agtttttata tagtttacca gttaatgctg aattttatga gaaaaaaaca
     3541 tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt aaacatgatt
     3601 ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac gtatttttta
     3661 ctttttattt ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca
     3721 aaaaaaatat gtatcataat aatttttgta tcaatttttt ctttgatact tcatatttta
     3781 atcaatcaaa tgt
//
LOCUS       DANJLF010000139         3765 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000139 DANJLF010000000
VERSION     DANJLF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(684..977)
                     /locus_tag="PWT25_002763"
     CDS             complement(684..977)
                     /locus_tag="PWT25_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL1022770.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1380..2558)
                     /locus_tag="PWT25_002764"
     CDS             complement(1380..2558)
                     /locus_tag="PWT25_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1022771.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2662..2976)
                     /locus_tag="PWT25_002765"
     CDS             complement(2662..2976)
                     /locus_tag="PWT25_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022772.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3083..3301
                     /locus_tag="PWT25_002766"
     CDS             3083..3301
                     /locus_tag="PWT25_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022773.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(3296..3691)
                     /locus_tag="PWT25_002767"
                     /pseudo
     CDS             complement(3296..3691)
                     /locus_tag="PWT25_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1204 a          721 c          553 g         1287 t
ORIGIN      
        1 ataaaaaata ctcttttttt acgattattg aaaaaaaaag ataaaacttg attaattgaa
       61 gtattgctta aatttggaag ctatgaggga agaataaaag agttaggacg ggattatcta
      121 ctatttttct ttgttttaaa tatttgttat ctttgaacat aaaagagaaa tgtgtcgact
      181 gcagtgttgt gtccctcatc acgaataaac ggtgttcaat tagctgatct tgtattcact
      241 taaaaaatgg taaagtacga gttgctattc cccattttca attttctaac gctcaggaca
      301 aaatactgtt ttcacaacct cattggaata aaagcatata tattgtttca agaacgaaat
      361 cttgttatct ttttaaaggg catctgtcca aaatctaatt ttaggaggta cgttttggta
      421 tttattcgta caaataaaag agaattatct ttattcatgt aacgcatcca attgattttt
      481 tcatgtacgc tcctggaacg attttactga cgattgctta tttaactgcg aatcgctcat
      541 tagcttttat catagctatc catattttga ataacgttct tggctttgta ttatagatac
      601 agatgacaaa aaaagttcaa gtatgcggtg tagaaccaat atcgcatact tgaacttttt
      661 tcaatgagta taaattaaaa cttctaattt tcaattccca tcatctgata tgcccgaccg
      721 tctgaacttc ttttgaaaaa gccaaatgtg attagataac gtctttgttc gacataatcg
      781 ccactagaaa atacgttcag cagtatcgaa ttaacctctg tttctgtata agtcactcca
      841 tggtcaaagc gtttcaagac ttctttcaat aggatgtatt tcttttttgt tgaacttggt
      901 acttgtttta cttttccatc attcatgaat gtgtctaaaa ctttcatctt ttcttgctcc
      961 gtataagtat aaaccatctt ctttctcctt ttgattctaa gaatactcca tttatgttag
     1021 aatattctgg attttttatt aaagcggtta ctcttaagaa agatattatc atagtgctct
     1081 tttttttact actacttata ggtagattta ttctaatcca ttattctccc ctaccaataa
     1141 ttaaagaaaa aactggatca gggtgttcaa attacatgct aagaaaaaag aacccttgaa
     1201 taccaagggt tctttctcag tctgataaca atgattaacg acggatttct ttgatacttg
     1261 tgaaaagtcg ttaaagtatt gatataaaag gatttttatt acctctgact acatattgac
     1321 taatttgtta aaaaatcgtt tttcaagaat cctgtaatac cagtgctttc accgtttttt
     1381 taactctcta aagaactaaa agcaatcaga ggttttacct tttcatttga cgttaagtgg
     1441 cgataaattt tcgaagttgt ttggtcagta tggcctaagc gatctttcaa gacttcccag
     1501 ctacagtgtt ccgctactaa gcttgccatt gtatgacgag ctaaatgagt atgaaatggc
     1561 ttattaattc ctgagcgatt cacgcaccgt ttgacgtgat catttacata ctctcgacga
     1621 taaggaacac cacgtttact agaatcaaga tacacaaaca aatatgtttc ttctataata
     1681 aacctgtaat gttgcctcat ctcacccaaa gccttaatca actgttgtag cttttccata
     1741 actagaggag ttactagaac tcgtctagca gaactaactg tcttagtagt ttcaattcga
     1801 aaatccttac tcttaccttt aactgccgtt tgcttataga acagaatttc atttcttttg
     1861 aaatctatat ctccaggctg tagagcaagc gcctcgctga ctcggcagcc agtgtaaaat
     1921 agaacatgga aaagtacaga catcggtaat tcctcatgaa caatgccata attcaaaaaa
     1981 gttctcaatt catcgacagt aagaaacttg tcctcaatat tatttatggc ttcttttaaa
     2041 tcagttaccg ttaactgata ttttggcatt ctcacagact tcattggatt ttcacttatc
     2101 aaattctttc tttgacaata tttaaaaatc atatttagaa cttgaataag tgattgtaaa
     2161 ctatttttgg catagccaac atttgttcca ttaacgccac attcctttaa ggtaaaaagc
     2221 aattcttgaa ggcactctgg tgtcaaatca gacaactttt tatcaccaat ccatcgaaca
     2281 ataattttta attgttcctc tcgcttatca taggttattt ccttttgttc atttaacgaa
     2341 cggtaatatt ggaaccattg ctcacctaca acactaaact tgtcattgct tgcaacaatt
     2401 tttttcttaa gcctttgttg tttctccatt tccagataag cgagttgcgc ctcacgtttt
     2461 gaattaaagc cacgtcttgt tgttcgatat gcttgaaagt accatgtgat cctaccagta
     2521 gaactgattt gcttcttgaa tcgatctgtt ttttccattt attgattcct cctattttga
     2581 aggaacccaa caagcagaaa ccctttggaa atctttctat ctatgtcatc attgtaccgc
     2641 atcttagaaa gaaaatgtaa cttataaaga tcgaagtagt cgcaattttt ctcgatagtc
     2701 attcgaatca ctctttctca tccattctaa aacatcatca cgaaaatagt gtcctcgaat
     2761 cccgtaccaa cagggaggaa actcaccaag ttttactaaa cggttcagtg tactatcact
     2821 tatctgaaat agtcgcttta aatcagtatt tgttaatacc agaggtaaat gactgactgc
     2881 aagcaatttt tccgtgtaat cttccagttt tttttgtaaa attgaataga actctatttc
     2941 cagagaatta acatttgttt taacttccaa ctccatcacc cttctctctc aaaatcaaaa
     3001 cgaccaccac ggtacccctt aaaattgtaa aaaaacactc cagtactcca tcaaaacgga
     3061 ctagaaaaga ggaattttta atatgaaaca aggaattgca gacataaaaa taattaaaga
     3121 aattcttgaa aaatctacgg ctaatgcaat agcattcgga acaggaatca acttgagtac
     3181 agtaaaaaaa cttaaaagtg gagagagagc tgaagaaaaa ttaaatcttg ccgatgctat
     3241 taaaatcaca gaatttggca tgaaaaatat gccaaccaaa atcgaaatat ggaaatccta
     3301 gctcttccaa agccatttgt cgttggtacc aaacactgcc gagacttttc ccaataatat
     3361 cgccaatttc acgatcatta agaccgatga agaaggacat aaaaatgatt tcttgtcttt
     3421 gactaggtaa tgctgcaatc gcatttgcca actcctcatt ggaaataaag attttcatac
     3481 caaggacaat aaaagtacca ctactattca aatttggttc caaataatag aactctgtac
     3541 ctaacccttt ttgttccaat atttccattg atatttgccg ttggcgaaat ctagcgtact
     3601 tcttttgaat atttaacgcc tcattgcaga ctactttctt acaaaaactg tcaaacgtat
     3661 gttgaatgtg ggtttcctct gagtcgccca taatattctc cccctcaaaa aattcattca
     3721 actcgtttta cttctatccc tttccattat tactacaatc tagga
//
LOCUS       DANJLF010000140         3758 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000140 DANJLF010000000
VERSION     DANJLF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..994)
                     /locus_tag="PWT25_002768"
     CDS             complement(<1..994)
                     /locus_tag="PWT25_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1022774.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAA"
     gene            complement(1143..1322)
                     /locus_tag="PWT25_002769"
     CDS             complement(1143..1322)
                     /locus_tag="PWT25_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022775.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1377..1460
                     /locus_tag="PWT25_002770"
     tRNA            1377..1460
                     /locus_tag="PWT25_002770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1411..1413,aa:Leu,seq:gag)
     gene            complement(1700..3070)
                     /locus_tag="PWT25_002771"
     CDS             complement(1700..3070)
                     /locus_tag="PWT25_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022776.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
BASE COUNT         1250 a          738 c          572 g         1198 t
ORIGIN      
        1 aagcagcttt tggttttacg gctgaaagac caggaatatc attgattgca ttatagatat
       61 attctctttg ttcatagatc cttccgcctg gcaggagcag ctcatcgaca ctttgataac
      121 cgcctagcgc ggtttggata atttgctgag acaatacatt ggaacacaaa cgcatggagg
      181 acaacatatt cagcccttct atatagcctt tgacgtgtga cttatctcca cttaggacca
      241 tccagcctac tcgaaatcct gctacacgat gagatttgga tagaccgttc agcgtaacaa
      301 cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc tagaccatcc atcactaagc
      361 gatcatagat ttcgtcggaa taaataatca ggttattttg tcgagctact tcaacgattt
      421 cttctaataa ttcttttgga taaagtgccc cagtaggatt attaggatta atgataacaa
      481 tagcttttgt ccgactcgtt atttttgatt tgatatcatc aatatcagga taccattctg
      541 cctcttcatc gcaaatataa tgaacaggtg tgcctccagc taatgcaata gaagccgtcc
      601 acaacggata gtccggcatt ggaaccagta cttcatctcc attgtccagc aacccctgca
      661 tacacatcgt gatcaattcg ctcactccgt tgccggtgta gatatcattg atcgtcacat
      721 tcggaaaatg tttcagttgg caatattgct caattgcttt tctagcagaa aagatcccct
      781 tggaatctga gtagccttct gagtcacggg cattcatgat caaatcacga atgacttcgt
      841 ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt caattttaaa atactaatgc
      901 cctcttcttg catccgctcc gcttcttcta gaacgggacc gcgaacatcg taactgacac
      961 cttctaattt gttggatttt tcaaattctc tcatcactta gccaatccct catttcaaaa
     1021 agttagaact ttccaaattt tttgttattt actatttcaa tatcatactc catcctagaa
     1081 aattttgcca cttatttagg aaaaacgtaa gtattaaaaa aacacaaaca agcgttgaga
     1141 acttaattgt catgatagtc attccataga ttcgctgccc aatccattgc aattccacaa
     1201 atgattccta gcataaaacc aagcaaaaaa cgtccgaatg caagtccaat caatacacct
     1261 aagatcattc ccagtcctga aaattctccg tcatatttct ccatcttaat aagtcctccc
     1321 atttttgagc ttgacaacta tgtaaaacct cgctatcatt agtgtgttat tattacgcag
     1381 atgtgttgga attggcagac aagcatgatt gagggtcatg tgagcgttag ctcgtgtggg
     1441 ttcgagtccc atcatctgca ctacaactcg tgaagttaat tttgcttcac gagttttttt
     1501 gtttgaacgt ttctttatgc cattatatac atatcactgt atcctttaca aaaagaacgt
     1561 acaatcatgt aaacagcctg aggtgagtat atgaaggtcg gaaacaagct actcgtagaa
     1621 acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa atccctcacc taatactaaa
     1681 gcgagggatt ttttgtatat tatctgttta tcaccgttat tataaaggta caaataccaa
     1741 gcacggttaa tccaaattgc ataaattcat ttttttgtac actcatgctt ttcattagct
     1801 ctttaactgc cattagtact aagatggcga taacaacata aagtatatag tacgtccgca
     1861 ttccggaagc ccatactgtg ggagaaaatc ccataattaa ccttgaacaa aatcccatta
     1921 tcatcaaaat aactaaaaaa atagaagttt tcttattgtc aaaacttaaa taaattccaa
     1981 caattaaaca tagtaataat gccaagacta ctaaagtagc tatccaagta cctggataaa
     2041 aaagtgacag tttcgttcct aactctgtaa agagattatt caggttacta ctactccaaa
     2101 tcattgccct tttatttcca ttgacgtaag taaagctttg acccattgta ttaccaaaat
     2161 aaataatcag gtttaagaaa aacggtaatg cagtcaatat acgcacataa tagttttgac
     2221 atttcctata tgttagaagg aaaattaaga aaaatagcaa taaaaataag atattcatgt
     2281 ctaaaaaaaa cggctttcca aatgatgaaa tacctaaatc taacttatta agaaagttga
     2341 aatttttata ttcaggaaac catttattaa tttcatgtgc agcacgtaat gcattccctg
     2401 gagttgtcaa agagaaaatt aattctgcta agcttattat tgaaaatact gataacttat
     2461 aattatatcg tccattgaca atcaaatata aactaactat acttgttaga acaaaaaaac
     2521 aggcgttgac ttgttcttga ttagttaaaa atatcaataa aggaatactt acccaatata
     2581 tttttctatt tatttcttct cctcttaaga gttgaaaaaa cggataaaaa gttagtaaac
     2641 taaatgctac tggccactga tagttcaagg tggttgctct ccaaccggtt tcccccatta
     2701 ctgtcaatgg tattaaacag tatatagagc aaaccaataa taaattttta acatttattt
     2761 tctcaaaaac atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata
     2821 atttccatag cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg
     2881 aactccatgt cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac
     2941 ttgcataaac tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga
     3001 taccccaaat ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat
     3061 gtttgttcat ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc
     3121 attacttaaa gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt
     3181 gatgcaaaag aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt
     3241 tcttagttaa ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga
     3301 catacaatca tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa
     3361 tccaaatgtt aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt
     3421 caatttactt ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg
     3481 aacgtttaaa atcctacaat tccactacct gagactagta atgactagtc tctttttatg
     3541 gcctctgatc cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt
     3601 taagcaatat ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata
     3661 catgtagtta tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc
     3721 atcgtctgca tccatctttc caaaacaagc ggctagga
//
LOCUS       DANJLF010000141         3713 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000141 DANJLF010000000
VERSION     DANJLF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="PWT25_002772"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="PWT25_002772"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL1022777.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="PWT25_002773"
     CDS             complement(2261..2512)
                     /locus_tag="PWT25_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL1022778.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="PWT25_002774"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="PWT25_002774"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL1022779.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="PWT25_002775"
     CDS             3462..3680
                     /locus_tag="PWT25_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022780.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DANJLF010000142         3536 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000142 DANJLF010000000
VERSION     DANJLF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(45..1511)
                     /locus_tag="PWT25_002776"
     CDS             complement(45..1511)
                     /locus_tag="PWT25_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL1022781.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="PWT25_002777"
     CDS             1898..2494
                     /locus_tag="PWT25_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1022782.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="PWT25_002778"
     CDS             complement(2552..3034)
                     /locus_tag="PWT25_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022783.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="PWT25_002779"
     CDS             complement(3140..3337)
                     /locus_tag="PWT25_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022784.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT         1200 a          708 c          578 g         1050 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaac
//
LOCUS       DANJLF010000143         3461 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000143 DANJLF010000000
VERSION     DANJLF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(681..1295)
                     /locus_tag="PWT25_002780"
     CDS             complement(681..1295)
                     /locus_tag="PWT25_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1022785.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1901..2278)
                     /locus_tag="PWT25_002781"
     CDS             complement(1901..2278)
                     /locus_tag="PWT25_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022786.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2578..3459)
                     /gene="galU"
                     /locus_tag="PWT25_002782"
     CDS             complement(2578..3459)
                     /gene="galU"
                     /locus_tag="PWT25_002782"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL1022787.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1050 a          691 c          512 g         1208 t
ORIGIN      
        1 ataatttatt agaaaatcac ttttttctat tttgtttttt ttctcctatg tatgattatt
       61 tttttctctt tccatttata ttaagtattt tatttctctg tttttcctta aaattctttt
      121 tttattttta gattagagaa aatctcgtcc tgtttttttc attttacaat cattgaaaaa
      181 ttgaatttca ttgcaaaaaa aataataaac acttataaat atccattatc tatccttcaa
      241 tttttgtgta ttttatttct attagaaaaa ggtatcctgt tggacaattt tgttatatca
      301 ggtaaacttc attgcaataa tgataacaaa tgtactataa ttactttgta aagagttttg
      361 gggaagactc tttcatcaac gtgtattaag agacaagaaa gaatccatat ggattctttc
      421 ttgtctcttt ttccactact ttttattttt ctgacactac atattcttat aactcatttc
      481 tcataactgt gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca
      541 aagatgatcc atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa
      601 taatcgtttt ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa
      661 ctttgtcaac atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta
      721 aaatacaaaa cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct
      781 ggatctagat ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc
      841 aaatcagcaa attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac
      901 aaatcaaatc cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt
      961 tcgatgattg ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg
     1021 acagaaagct gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg
     1081 gctggatttt caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat
     1141 gctacaccac ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt
     1201 ttcgcgatcc aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc
     1261 aattggtgat aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt
     1321 gacatgaata gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca
     1381 gtaacacatg tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa
     1441 taccttgaca tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag
     1501 aattttcacc ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa
     1561 aatgatcggt cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa
     1621 gggtcaggga gccttttttt attcagctgt ttctatctga atttattgta agtaatctct
     1681 tatccaagtg caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat
     1741 aacataaaaa aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt
     1801 ttatataagt gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt
     1861 atccattatt ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc
     1921 ttctactttg ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc
     1981 ctatccgaga ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa
     2041 ataaagcaaa tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa
     2101 ccccaacgga tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc
     2161 gaaacatgat aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt
     2221 ccatcagcca cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag
     2281 tgaacacaca ccttttttta taattaaaaa attcgccttt ttattctact atgaatcttc
     2341 tggcttggct ttcatttctc ttatgtaatc attttctttt atacaactta aaaaaaaatt
     2401 aaaccattag cataatattt tctgaatcta ttcacgaaag tctttttcta tctttcgttt
     2461 tccacgactc aactgatcta gagtcaaaaa actaaaacga cagttgcgca aatgacgcac
     2521 aagctgccgc tttaataaca aatagccttt cattctcagc aggccactta tttccagtta
     2581 actttctgtt cttagctttt tactcaactg gataatatat gcacttaatg aagtcccaat
     2641 ttccgaatgt tgaagtccat attcaatatt tgtcttcata tatcctaact tatcgcctac
     2701 gtcgtatctt tttcctttaa actctctcgc atagaccgcc tctgtctggt taagagtctg
     2761 aagtgcatcc gttagctgta tctcgtttcc tgctcctggt tcttgagttt ctaatatctc
     2821 aaatatttta ggagtcagta gataacgtcc gataatggct aaattacttg gtgcttttcc
     2881 catttcaggt ttttctacaa acccttttac tctgcaacga cccttttcgc tatctgtttt
     2941 aggatcaata attccgtatt tagatgtttc attttctggt acacgcatga cggcaacagc
     3001 ggaagcttgt gtttcttcat aatccatcat caattgttga gtcaatggga tatcatcttt
     3061 catcaaatca tcccccagca tgacgacaaa cggttcattt cctataaatg cttttgcttg
     3121 aagcacggca tcccccagtc ctcttggatg agattgacgg atgaagtgaa gattgacttg
     3181 agtcgtttct tcaactaaag cgagtaattc tgttttttcc ttttcccgca aattgttttc
     3241 caattcgata tttgcatcaa agtgatcttc aatgggtctt ttttcttttc ccgttacgat
     3301 caagatgtct tcgattcccg aatccaatgc ttcttccaca ataaactgga tcgttggttt
     3361 atccacgatt ggaagcattt cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc
     3421 tagcccagcg gcaggaatga ctgctttttt gaccttcact t
//
LOCUS       DANJLF010000144         3399 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000144 DANJLF010000000
VERSION     DANJLF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..797
                     /locus_tag="PWT25_002783"
     CDS             <1..797
                     /locus_tag="PWT25_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1022788.1"
                     /translation="ENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNTKVGDATII
                     RFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEK
                     LNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTV
                     NDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLN
                     GGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            complement(1480..1578)
                     /locus_tag="PWT25_002784"
                     /pseudo
     CDS             complement(1480..1578)
                     /locus_tag="PWT25_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1843..2298)
                     /gene="tnpA"
                     /locus_tag="PWT25_002785"
     CDS             complement(1843..2298)
                     /gene="tnpA"
                     /locus_tag="PWT25_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL1022789.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2541..3399
                     /locus_tag="PWT25_002786"
                     /pseudo
     CDS             2541..3399
                     /locus_tag="PWT25_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1127 a          585 c          591 g         1096 t
ORIGIN      
        1 aagaaaatta tccattttta acgaagtctg aaaaaaaagt cgctgatttt attttaaatt
       61 ctgggaaaac tattatttat agtacgatga gtgatattaa gaaaaacaca aaagttggcg
      121 atgctactat tattcgcttt tgtcaaaaaa ttggtttttc tggtttttct gatttaaaaa
      181 ttgaaattgc aaaggaagac tttagtcaaa aaaaagaaca tccgactagt gtaaagtact
      241 atgatgaaat tgctaatagt ttaacagaag ctctctattc cactattagg cttttagacg
      301 aagaaaaatt gaatgaagcc attaaaatga tcactcaatc aaaaaacata tatatatttg
      361 gagtgggatc tagtggaaat actagtcttg atttagaaaa tatgtttctt cgcgtaggag
      421 ttcaagcaaa agctgtacta gaccctcatt ttcaagccca agtcgcttct ttattaacag
      481 ttaatgattt agtaatcatt ttttctttat ctgggaaaac gaaagatact tatgattcat
      541 tgaaaatagc taaaaaaaat ggcgcaaaaa ttattgctat aactaactat attcattcac
      601 ctataggaaa gtctgcagac ttagttttac aaacagccat tgaagaattt cttaatggtg
      661 gatctttagc tggaaaaata tcacaacttt atatttgtga tctattagtt catggttatg
      721 aacaaaacaa taatattgat tcagttgaat taagagaaaa agttttgcgc tctattattg
      781 ataaaagtat tgagtgaact acccccactt agctaaacct aacggttttt aacgattgaa
      841 gtaggcaatt cttgggatga gagcatactt gatagcttat tttttactaa tagcggtggc
      901 tcttattgac caaatatccc tgtagttcct acggttcttg attttatcta aagatatttt
      961 tatcgtggaa gctattgatc aacttgaaag aaaaacgtgt agctcattat cactagttta
     1021 tcgagtatgg atgaagcaat ggatcatcct ctcttaaata aatttattaa ggatctgatc
     1081 cttcaaattt tttccatgat cgatgagcag gaacctactg aatctaaacg acatcgggca
     1141 caaggaatta ttaagattgc taagcgaatg ttttctataa agggtgttct aagctataca
     1201 gtgttaattc aaaagattcc caatcatctt gtttataaga acaacgttga agattaaaaa
     1261 agtgtttcaa tgctaaaatt gcaaaatact tcaatgttat gagacaaact gtctataaga
     1321 atcaatttcc acccatttca tcacttgtta aaagtttatg atccttttga tttttatatt
     1381 aatttatctg tatttaatac agattttatt tattagttca tctgcctatt gtattttatt
     1441 ttgttgcctt accatctgaa gtaaatatac tcttaattgt caaattaagt tagacagatc
     1501 atcaatactt acgtaactca aaaatacttc caaaggtgtt cgataatcca gtgattttct
     1561 aggaagatta tttctgcgcc agcattttct aatgtttgaa tttgtgtttt gtaatcttgg
     1621 ttgcttgtgc taattcttga atagccgtaa attgccaaaa tttcatcacc ctttctggac
     1681 ataagtttct gacaccagtt aaaatcaagt ctatcagtaa cccatacacc cgtgaggacg
     1741 ggtgtatttt taatccccct agaaggggtc gacactcgct gccccttaaa aggggatttt
     1801 taaaagaccg ccaactgctc atcttcactc acttacttct ctttattttt taaatggttc
     1861 tacgtactct cttttactta taatatatcg aatgcgatca tcagcttctt gttcacggat
     1921 gtatttttca tttgtctttt gattcaatcc gactgtactc acatagtatc tcttcgacca
     1981 aaaggatttg tttccgtatt tatatttgag atttgcgtgc tgctcgtgga ttagtaccgc
     2041 actacgacct ttcagatacc ccataaaact ggatatacat atttttggtg gtactcgaac
     2101 aagcatgtga atatgatcta gcattgcatg tgcttctatg atttccacat ctttcatttc
     2161 gcacaacttc ctaagtattt ttccaagatc ttttcgtaat tgcccataga ttacttttcg
     2221 ttgatatttt ggtgtaaaaa taatatgata cttacaattc caacgagtat atgctaaact
     2281 tttatcgtca tttgacatga aaaaactcct ttcgatctga tgagcggttg gtagccactc
     2341 actagtatat cgaaaggagt ttttatcgct aaagatactt cttccacgcg tagaaacgcg
     2401 cggttttctt gtttcgcacg ctttacgcac aaaactgact gaagtcatta agaaaataaa
     2461 agggtctaaa acttttaagt tttgtaccga cccccaaaag ttagaccaaa aaatctaact
     2521 tttggaggtc ggtatttttt atggctaagt atacttttga acttaaatta aagatcgttc
     2581 atgattactt ggatggtaaa ggcggaagtg attatctggc aaagaaatat tccattaagg
     2641 ctccatcaca agtgaagcgc tggatcaatg cctatcaaga atttggtgaa gagggtctag
     2701 taagaaagcg acaaaagaaa aaatattctg ttcaatttaa gctggatgcg atagagttat
     2761 atctaacaag tgagctgtcc tatcgtgaag ttgccaacac acttaacatg aacaacccta
     2821 atttgatcgc tagttggatg cgtcaattcc gtgagggtgg gatcgatgga ctctcaaaaa
     2881 cgaaaggatg tccacctatc ttgtctaaga aaaatgaacc caaaaataag aaaaaatcta
     2941 caactaaagc aacgtctaaa gaacgtgaac gcatcgagga gttggagaaa cgagtccgtt
     3001 cccttcaaat tgaaaatgcc tttttaaaag aattgaggaa gctgcgaaaa caggaagccc
     3061 aacaacgacg aatgaagcaa tcgcacgaat catcgcaagc ctccgaggac cgtttaaatt
     3121 agttgagctt ctagaaactt taaagttccc taaggcaatc tttatgtact ggcaaaaacg
     3181 attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaag ttcgggagaa
     3241 gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg gtttctctat
     3301 caataaaaag aaagttcagc gcctgatcaa gaagctagga ataaaagtaa cgacatacac
     3361 acgtaaatca cggcgctaca attcttatcg aggacaagt
//
LOCUS       DANJLF010000145         3366 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000145 DANJLF010000000
VERSION     DANJLF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            116..1156
                     /locus_tag="PWT25_002787"
     CDS             116..1156
                     /locus_tag="PWT25_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL1022790.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            1980..2312
                     /locus_tag="PWT25_002788"
     CDS             1980..2312
                     /locus_tag="PWT25_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL1022791.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2931..3272
                     /locus_tag="PWT25_002789"
     CDS             2931..3272
                     /locus_tag="PWT25_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL1022792.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1255 a          457 c          640 g         1014 t
ORIGIN      
        1 gtatttaatt aaaaaacagt tttaatgcgt ttgcattttt tatttatctt tgctattttc
       61 gaaaagagta ggtaatttta cataaataaa acaacgtgaa agtagaggat ctacaatgac
      121 tgattttaaa ttttttaagg cggatagagt ttacaacgaa ttattttatc aatttccaaa
      181 agtctttatt gtttctgacg aatacaaaaa aatgaaagat tcaactaaga ttgcctatat
      241 gcttttaaaa gcaagattag agatcgcaat cagcaaacgg caaatcgatg aagaaggtaa
      301 tgtttatttt acttatacga caaatgaact atgtagagta ttaaactgcc aaaaacaaaa
      361 agcgatagca atcaaaaaag agttggaatc ctttggttta ttattacaaa agcagatggg
      421 atttaacaaa cagttaggga aaaataatcc taatagacta tatctagcag aattaaaagt
      481 ctcagaaaat gatatctact tactcgaaaa atttgataga gagaataggg aaaacgttga
      541 taaatcagag ggtatgaaaa tcatacccac cctcgacgaa aaatcagacg ctgaatccct
      601 tggggctcaa gagggtatga aaatcatacc gtgccaaaac gttgataaat cagagggtat
      661 gaaaatcata ccagaactta ataataatat attagacact aatagacaca atatagacac
      721 tgaaaaagac cgcctacaag atcaattgtt gttagacaat tttgagacaa ttatgacaaa
      781 cgacagcatt gctacgtttg tccctgaacg agtattaaat ttgataaaaa cattttcttc
      841 aagttacagt gaagctcaaa aaaccgtcca gactattcat aatgcaaaga aaaaagctga
      901 aatagaaagt ggtatttcga tagtttttga agaactcgat agttattatg tcaatgcaga
      961 acaagaatta tacacgacac tgttaaaagc ctatcaaaaa ttaaaaaccg aaaaagtcga
     1021 aaatatccag aacctgattt ttgtctatgt aaaaaattgg tttatcgaaa aaccaatagc
     1081 tgctaaagta tcaagtgaaa aacgtttgaa ttatgaaagc tccccaagca ctattacgaa
     1141 agactggtta gagtgaaact ttactgacaa agtgattgtt gcttcatgga gtaacattaa
     1201 aattaaaaat tttttattat tctaattata ttacgaatat tacaaaataa tagctatttt
     1261 aatagaattt ataagttaaa atattatgat aaaaatcaca aacgtagcct tttacgtcat
     1321 aagtgttaaa agaaataatt aatttcaaat atagaaaagg gaacgtatgt ttgcatactc
     1381 cttttttttg agttataata atacgcgaag agagataatt tcattattta aacaagctaa
     1441 aaatcttgat attacttctt ttcaggcaat acgaatatta ttcgcttaca ttttttgtat
     1501 aaatttaatt gtctgctatg cttgtgatga attgattttc gttctgcact tttggatgat
     1561 aaatttggac aattgaaaat acaaaaaaaa gaaaggcggt atgaactcgg aaggctggta
     1621 accttcgtca gcgttcatac acggcattaa gatttggccc ttaatgcgtt aacctttcag
     1681 tgttacggta ttacccgcaa tggatttagt ataacagata ttcaaaagtg ataccacttt
     1741 ttttggctgt gttaaggtta aaaatacgtg atattacgat gaatctatgc ctaagcacaa
     1801 tgttataaaa atgtccgtgt ggctctttgc cgtatgacga acgctgaaat tagagggaac
     1861 gcatgtttgg atgcgttccc tcttttttgt ttgtcatgaa cgaaaatcaa ttctctattt
     1921 ttgaaaaata acaagaaaaa cttgttaaaa atatttaaag aaattgggtg gtttatttta
     1981 tgtcatatac aggaattttg agtctcaaag atatttgtca ctacggtaaa cggtgtaccg
     2041 ctacagagaa aatcacgaag aaactatcaa ctggccaaag taaaaccgtt gtgcagtgta
     2101 agaaatatat aatccagaaa gacaaagtct cagaggaaat gatttattac attgggaaac
     2161 aaaagcaaat aattttaaaa gatcctatcc ccttaaaaga gctatatcca acaatcaaac
     2221 atgtttatga ccaaaacggt gtactgattg gtcgaagaaa aaatggagtc ttgcgttgta
     2281 ccgcaaaagg gatggggcgt ttgatcggct gaattgatca ttctcataac ttgtatttta
     2341 tcgtttttcg cttgcttgaa aatattgtcg agacttctaa aggaacttag agtcactaga
     2401 aaaataagga gtagacttga tctatcaaca attcccttcg gggagtgacg gggtaggttt
     2461 taacgattgg taacgtaaaa aattggtcaa tcgagtaaat gcgcttgttt ggatagtggt
     2521 gaaatgaatt tgtgtaaata ataagcgcag agtaaacaag agggaattta gaagttagac
     2581 aagttagaat agcgtctaga ttaatcatga gcagctacta agggatctag actttgacgc
     2641 aaactcatgg aataaggagt aggtcttctc gtaatgggga ggacgcacaa gaaattgtgt
     2701 cgtgtgctgc agtacatgta gatacttgcg aggacgttga ttattggatc aatcgagcat
     2761 ttaccgctgt ctactagggt ttctcgtttc ggacgccata aacgagtcga aattttttat
     2821 gtatttttaa gtaaacgaaa aacgataaaa tatgaacaag tcctgaataa agtgaaaaaa
     2881 ttgatataaa acatagagaa aatgaaattt acgtaatgga ggaatgacaa atgaaaaatt
     2941 atgatccgaa tatacgattg ggcacacata ctattaaggt ttctttccaa cgttgggatt
     3001 acaaaggatt tgtgacgttt agaagaggcg gaaattgtaa aggattagat gttttagcat
     3061 tagacgagga tgatttatac gatcaaacat tgactgataa tccaattgga ttcggcttgc
     3121 tacctgaaga tgatgaaggt aatgaatggt ttaaaatgac gcttatgaat gataatggag
     3181 acgaattatc tgttgaagat acttggtcat atttgagtga ttatattgtc agctttgaaa
     3241 tcattgaatt tgtagcggat aaagaagaat aaaaactgga ggaataaatt ttacagataa
     3301 aggcttagaa ggagtcacga tgattaataa aaaggtagta ttaccttagg cattgagtaa
     3361 ggaatt
//
LOCUS       DANJLF010000146         3355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000146 DANJLF010000000
VERSION     DANJLF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(40..903)
                     /locus_tag="PWT25_002790"
     CDS             complement(40..903)
                     /locus_tag="PWT25_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL1022793.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1099..3198)
                     /locus_tag="PWT25_002791"
     CDS             complement(1099..3198)
                     /locus_tag="PWT25_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL1022794.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT          746 a          670 c          575 g         1364 t
ORIGIN      
        1 attattataa gctagttaag aaaaacatgc aagggattct caattagatt ttttgactag
       61 ttttgtcaca ccgttttctt gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg
      121 cttaaatgct cctttgctga taccaaaagc tttcatgatt tcttctggat ccgatttatc
      181 tgtaaagttg atttgctgat ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc
      241 atctgatata gcttcatatg ctcttggctt caatgaaaga ttcagtacac cgtctggtcg
      301 gacaccaatc actcgacctt ttatctgctc gcctagacga ggttcattat atcgttcaga
      361 aggatggata aacccaagat agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa
      421 gcggtatgct gttcctatca catctttgtt tttcatttct tctgttccat atttgcttaa
      481 agatttgaat attttttcat ctgctaaact gccccaaata cggtcttttg catcaactct
      541 aagggaaatc atcaagcgat cccctttttt aggccatagc tctgtcattg tcggcaattc
      601 atcaagagat acgacaaagt ctttatctgg aagaccgata tcgacaaacg cacctaaatc
      661 ttttcgtgta gcagtaacga ttccaaaagc gtaatgaccg atacggcttt taggaatttg
      721 tgtagtgatc cgattttcat gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc
      781 gattttgtgt tcaccttctg atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa
      841 gtagtttttt tcattttcat caataataat acctgtaaat atagttgcta ataaatcgtt
      901 catttttttc tccctctata gtgaaaggtt ttcttaatac atttctttgc tatcataaca
      961 taatgttatc tacttgaata gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac
     1021 aacagttgtg tcatactccc ctttttcgaa taaacggtgt taaacggcag gctcacagcc
     1081 tctttcattt gcctaaaatt aattgctccc atttcctccg aaccatccag ttttacagtt
     1141 gatggttacg atgtaaaatg gcaaagcact ttctccaatg cctttcatca atgcagattt
     1201 tgttgaagtg gcatatagat tataatagcc ttccccattt tcgattctgg ctttttctaa
     1261 tctgtaacct ccttcaacga tatatccacg ttggatacat gtagcaatca ctttgctttg
     1321 gacacctggt gcccacgtcc atgctggatt agatgcatcg gcaatatcac ttgctctggc
     1381 agatttgatt ggctgattcg ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg
     1441 ctgtgctgct tgtgcgaccg cagcttgttg agcggcttga gctgcagctg ctgcttgagc
     1501 ggcttgtttt gcttgttctt cagctgcttt tttcgcttct tctgcttttt tcttttcctc
     1561 ttcttctttt ttctctttat cagtgagcat cttatctact tgtttcaatt gttcattcaa
     1621 ctgagcagat agatctgtat ttttcacttt ttctactgct tcttttgccg tcttgtattg
     1681 gtcgcgtgtt gctgaatcca ctacttttcc gtctttaaag actatagctg ttttttcttt
     1741 agctgtttgc aattcagcat attgattttt agcttctgtt tgtgcttgtt gaatcaattt
     1801 tccaaaatct gtgttatccg tttcgattgc ttggatctct ttatctgctt tcaacttgat
     1861 gtcatctgct agatgatcat cgacgatggc aggtgctatg aaccactcat tcactttttg
     1921 gatatctcgg atcttagatt ggatatcttg gtaagttgtt tctaactctg aatatccttt
     1981 ttcattctta agactggata aatcattttt tagttttgat aaatcttgat gaatcatatc
     2041 tgcacgaata aattgatggt gatcatctgt ataaaaggca gcaagtttct catgaagttc
     2101 ttccatttgt tgctgtcttt ttgcttctgc tgctacttcg gcttctactt tttgatgatg
     2161 gtcataataa gcccagcttc ctccaccgag tagaagtact gctgctaaaa cgagataagg
     2221 tacttttttt gattttttct tttgcgcttt atctggatct gttttttcta agtttgattc
     2281 ttgatgtgtt ttttcagtat ctggcacgat tgttattgat ttcgcttctg cttcaaacag
     2341 cttaggctgg ttctcttctt tttctatttt tgcttcaaga tcagtaccct ctttgcttac
     2401 agattgattt tcagcatcgg attcactatt tatttcatcg ggctctgttg actttttgtc
     2461 tgtgttttcg agtatttctt ttttctctgc ttccggcagt ttattcgttt tttccttttc
     2521 taattgtttt tcagtaaata cttcagtttt ttctgtgata tccgttttta cgtcttttat
     2581 ttttactgtt tctttacctg tggcgttttc tctatcagag tctttttcag tttcttgttc
     2641 cttttctggc tctactttgt tttcctgtgc aattttttca ctgatcattt tgagcagctc
     2701 tttcgcgtgc tcttcttttg tatcttcacg atgtgctttg atataagcgg atagtatcga
     2761 attttcttcc tcattatcga cgatttcttt cagttcagct actcttcttt tttgttttga
     2821 ttcaagcgtt tcttcttgat tctttcctga ttcttccgaa gtctcatgaa cagttgcttt
     2881 cttctgttca tttttctcta tattagttac ttgatcgtga gcaggtggtt cttcgtcttc
     2941 tggttctgct ttttcgaata attccattac agattctaga ggtaaatctt tcaattccga
     3001 ccattcgatt ggatcgttta attctgtatc aggaaagact gcatcttcag ttgatttatc
     3061 ctgcatagct gcatctgtat ttgtttcttc aattgattct tttatttgct gatcttctgc
     3121 agttatttga tgaccgcagt ctggacagat agtattctgc tccatttttt tcgatccgca
     3181 atttggacat tttttcatca cttagcgatc ggctccttta ctaggtttac gctaccattt
     3241 aatctatcat attctatacg tcacgccaat agcttatttt taggatttac ttaaaaataa
     3301 tgaatgaatt tccttcctat agaaaaaaaa gcttgagact gcgtagtctc aagct
//
LOCUS       DANJLF010000147         3221 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000147 DANJLF010000000
VERSION     DANJLF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            301..585
                     /locus_tag="PWT25_002792"
     CDS             301..585
                     /locus_tag="PWT25_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022795.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="PWT25_002793"
     CDS             588..1004
                     /locus_tag="PWT25_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022796.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="PWT25_002794"
     CDS             complement(1141..1860)
                     /locus_tag="PWT25_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1022797.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="PWT25_002795"
     CDS             2535..2630
                     /locus_tag="PWT25_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL1022798.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="PWT25_002796"
     CDS             complement(2932..3177)
                     /locus_tag="PWT25_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022799.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DANJLF010000148         3213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000148 DANJLF010000000
VERSION     DANJLF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..443)
                     /locus_tag="PWT25_002797"
     CDS             complement(<1..443)
                     /locus_tag="PWT25_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1022800.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="PWT25_002798"
     CDS             513..863
                     /locus_tag="PWT25_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1022801.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="PWT25_002799"
     CDS             1277..2095
                     /locus_tag="PWT25_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1022802.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="PWT25_002800"
     CDS             complement(2168..2527)
                     /locus_tag="PWT25_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL1022803.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT         1059 a          475 c          574 g         1105 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt
     2761 aaaacgcttc cttagtctat tatatatcta tttctcttag gagtatgttt agatgtttga
     2821 ataatagttg cccaacttta aaggttattc ttataatatt ttacttcatt ttccaataga
     2881 aaattcaaag gattactggt ttttctaaaa tagtttagtt taaatacata agtatcctcc
     2941 gacactgtat tagttcataa tcatctaaat acaaaatttt caaataaaaa tatttcaagc
     3001 tctatcgttg acgaatgtaa acgatactta taaagtaata gaaattgatt acatttgtaa
     3061 acttaaaatg gttaatttat ctcacataaa actaagaata aaataatgat taaaagtggt
     3121 tagtttttat acgatttagc ctatcggtaa aaatgaatta gagagtgtaa aatattttgt
     3181 gtaaatgaaa aaatccatac aaaaaaggaa gtc
//
LOCUS       DANJLF010000149         3206 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000149 DANJLF010000000
VERSION     DANJLF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            145..834
                     /locus_tag="PWT25_002801"
     CDS             145..834
                     /locus_tag="PWT25_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL1022804.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            848..2350
                     /locus_tag="PWT25_002802"
     CDS             848..2350
                     /locus_tag="PWT25_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1022805.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2363..2839
                     /locus_tag="PWT25_002803"
     CDS             2363..2839
                     /locus_tag="PWT25_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1022806.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2936..3046)
                     /locus_tag="PWT25_002804"
                     /pseudo
     CDS             complement(2936..3046)
                     /locus_tag="PWT25_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1058 a          433 c          618 g         1097 t
ORIGIN      
        1 tttcaacttt tctccattat actccaaaat aataaaaaaa actcctaaat actccatttt
       61 tatctttact tttatgaaag cggatgctat attgtttttg gagttgttat ccaaaaataa
      121 taaaaaattg gagtgtttag gataatggat aaaaagaggt ttttagatct tatttcaaaa
      181 ggtttgattg tttcctgtca agcattacct ggtgaaccat tttacaaaga ggaagaaagt
      241 ttaatgcctt attttgcttt ggctgctcaa agggctggag cagttggtat acgtgcaaat
      301 tctgttcgag atattattga aattagaaag tgtgtatcat taccaatgat tggaattatt
      361 aaaagagatt atccaccaga atctccattc attactgcca ctatgagaga agtagatgaa
      421 ttagtagaaa ccggtgttga ggtaatcgct cttgattgca ctcttagaaa tcgacatgat
      481 ggactatcta taaattcttt tattactaaa ataaaagaaa aatatcctca tcagttattt
      541 atggcagata tttctacatt tgaagaaggc aaaaatgcat ataaagcagg aatagatttt
      601 gtaggaacta ctttaagtgg atatactgag gaaagtaaaa aacaagatgg gccagatata
      661 gatttgatta aaaaattggt agaagaaggg atttgtgtaa ttgctgaggg aaggattcat
      721 actccagaac aagcaaaaga gataaaaaaa ataggagttg caggtattgt tgtgggtggt
      781 gctattactc gaccacagga aattgcatca agatttgtta aagcattaag ttagggggag
      841 aaataaaatg tttaaaaaat tttcacaatt aggacgcgct ttcatgttac caattgctat
      901 attaccagta gccggattac tattgggact gggaggagcg ttaacaaatg agagtgctat
      961 aaatgcttat ccatttttag atcaaccgtg gttgcataca attttaagta ttatgagtta
     1021 tgctggaaat gcagtttttg caaacctagc actaattttt gcaattggta ttgctgtagg
     1081 acttgcaaat ggagataaag gaacagctgg tttagctggt ggagtagctt atctagtata
     1141 tactgcaact attagtgggt ttcttgaact tttttcagca aaagatgcaa cactagatac
     1201 aggtgtagtg ggatcaattg ttgttggtgg tactgttgca tttttacaca atcgttatag
     1261 aaaaattgaa ttaccacaat tcttaggttt ttttggagga tcaagattta ttccgattgt
     1321 ttctagttta gctgcaatca ttattggttc tttcttttat ttaatttggc caccaattca
     1381 aaatggttta gtagttgctg gtgaacaaat tgcccaaatg ggtagtttag gaactttttt
     1441 atatggtttt ttattgcgtt taacaggtgc tgttggacta caccacacca tttacccttt
     1501 gttctggtat acatctttag gtggtaccga aactgtagct ggatcaacaa ttgctggagc
     1561 ccaaaatatt ttctttgccc aactagctga tccaaaccac actggtctgt ttacttatgg
     1621 tactagattt tttgcaggaa gatttgcaac aatgatgttt ggattaccag cagcatgtta
     1681 tgcaatgtat cgtgcaattc ctaagaaaaa tcgtaaaaag aatggtgggc tttattttag
     1741 cggggccctt acatcatttt taactggaat tactgaacca gttgaatata tgtttttatt
     1801 tgttgctcct tggctttatg tgattcatgc attcttagat ggattatctt tttattttgc
     1861 tgatatctta aatattcgtg tagggaattc cttttcaggt ggtttgatcg actttttatt
     1921 attcggtgta cttcaaggga atgacaaaac taattggtta aaattaattc cttttggtat
     1981 tgcatgggca tttatatatt tctttgtttt tacattcttt atcaaaaaat ttaaggtagc
     2041 tattcctgga atggaagaag aatcagaagt tcattctgaa aaagtaataa acaaaaatac
     2101 atcattacat gaagagtctc ttatcattat tgaagctctt ggtggaaaag aaaatattga
     2161 gtctgttgaa gcttgtgcaa ctcgtttgcg tgttgctgtt acgaatgggg agattattca
     2221 aaaacaaaca ataaaagaca ctggtgcaac tgctgtcttc gaagtgaagg gtggcatcca
     2281 agctgttttt ggagggaaag ctgacttatt aagtcaagaa atcaatcaaa tattaggaaa
     2341 agataattag gaggttttta atatgtttga aattttcaaa aagaaaaaac aagaaacttt
     2401 ttatgcacct tgttccggaa aaatcatttc catcacagaa gtaaatgatc ctgtcttttc
     2461 acaaaaatta ctaggtgatg gttttgccgt catacctgat ggttcaaaaa tttatgctcc
     2521 attggctggt cgagtaacaa atatatttcc tactaaacat gctattaata taaaaagcaa
     2581 ctcaggcatt gattatttga ttcatattgg aattgataca gttgaattac aaggagagcc
     2641 tttttcgatt tgtgttcaag aaaatgatga ggttactcct gaaacctcgt tggttgatgt
     2701 ggattttgac aaaataataa aatctggtaa agatacttct gttatagttg tttttccgga
     2761 gaaaaatcag ttcaatgatt taataataga taatggtcga attgctcatg ggaattcatg
     2821 tggaaaaatc actttttaaa aaagtgtata taaaaagcta taaaataagc taaataggat
     2881 atttgtcaaa tgaagttggt gaacagaaag aactgttttc tttctgttca tttttttatt
     2941 ttattagtaa tgattcccca tgccaaatca tgatccgtaa tttaaagtta taaaagcttc
     3001 gaaatccatt ggcatttcgt tggaatacct tgatatgatt attcaaatat aaaatataga
     3061 gagaagagag agagggaaga tatttataaa gggcaaagtt atgtttactc aacttaggaa
     3121 atagaaatag atcaatttat attctcttgt caatcgaata tgaaccttag cactatatac
     3181 caaatatagt aaaatgctga aagatg
//
LOCUS       DANJLF010000150         3129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000150 DANJLF010000000
VERSION     DANJLF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            103..1347
                     /locus_tag="PWT25_002805"
                     /pseudo
     CDS             103..1347
                     /locus_tag="PWT25_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="PWT25_002806"
     CDS             1360..1992
                     /locus_tag="PWT25_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1022807.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="PWT25_002807"
     CDS             2197..2424
                     /locus_tag="PWT25_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDL1022808.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="PWT25_002808"
     CDS             2493..2759
                     /locus_tag="PWT25_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL1022809.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="PWT25_002809"
     CDS             2749..3018
                     /locus_tag="PWT25_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1022810.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1102 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttta
//
LOCUS       DANJLF010000151         3085 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000151 DANJLF010000000
VERSION     DANJLF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..153)
                     /locus_tag="PWT25_002810"
     CDS             complement(<1..153)
                     /locus_tag="PWT25_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL1022811.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            1030..1284
                     /locus_tag="PWT25_002811"
                     /pseudo
     CDS             1030..1284
                     /locus_tag="PWT25_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1354..1866
                     /locus_tag="PWT25_002812"
     CDS             1354..1866
                     /locus_tag="PWT25_002812"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1022812.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1863..2900
                     /locus_tag="PWT25_002813"
     CDS             1863..2900
                     /locus_tag="PWT25_002813"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL1022813.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1106 a          551 c          600 g          828 t
ORIGIN      
        1 cacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt tattgatgac
      241 cacgaaagtg catggcgata tttgatgttg atactttatg cagtacccta tgtattcttc
      301 gctatcattc tgtggctcat agcattgttt tttagagtat ttaatgacct ttaggtagat
      361 ttatatataa aaagggctaa atacgcaaaa taccccctta aaatgcaaaa taaatgcaaa
      421 taagggggta actccttatg aaaaaacaac taatagtatt ataacacgtt tttatgccga
      481 gaaaacttat tggttggaat gggctatgtg ttagctaact tgttagcgag ttggttggac
      541 ttgaattggg attaatccca agaaagtacc aactcaacaa cacataaagc cctgtaggtt
      601 ccgaccaata aggaaattgg aataaagcaa cgaaaggagt tgagtaaaat gagtgagaaa
      661 acgaagaaaa tcattcgagt atcattagct ttttcacaag tagtagtgca atcattgata
      721 gtttattata cttaccagca atataaatta tctaaagaga attaagtttt gattatgtaa
      781 taagttttgg tactaaaaaa gacttgatct aattagacca agtcttttgt tagtgttata
      841 ttaataacaa aataaaaata agaagtcgct cactccccga ccaaagtttg tgagcaaaat
      901 aaacagtcga tgtttgtttc ttaattgtat atcttgatat taaacgattt aagattattc
      961 ttcaagatat atattcgcaa atgtaattgg aaaagcgact tcttaaacaa aattaaggag
     1021 tcgcttttta tggcaaaaat aacgaaaaat gagcaagaaa atttggtgga aaattacaca
     1081 aaaaaaccgg cgtactctaa tagccggtta gacgcacatt tcgtgtgcac ttctaatccg
     1141 aaattgagct ttgacgctat gacgatagtc ggaaacctta ataaaaacag cgctaaaaag
     1201 ctatccgatt ttatgagttt agatccacaa attagacttt gggatatact tcaaacaaaa
     1261 tttaaagcta aagcactaca agacccccta tgttaggcta gttgtcgtat aggataataa
     1321 gttataataa agactatacg gaggatgatt accatgacaa ataaaaacaa tgaacgttac
     1381 tctaccgaac tgaaagcgac agccatcaag aaaatgatgc cacccgaaaa tagggggatt
     1441 cgccaattaa gtcaaacttt gggcattcca gaagcgactc tttattcttg gcgtaaaaaa
     1501 gctagaattc aagggaatac tacacccgga acgaaaaaaa atgtcgaaaa atggtcaagt
     1561 gaagataaat ttttaattgt gatggaatca ttctcaatga accaagtaga acttgcggaa
     1621 tattgccgaa agaagggctt atttaaagaa caaattgagg cttggaagaa aacgtgctta
     1681 tctgcgaatg aacaggaaga aaatcgaacg agagaacttg caactgaact aaaagaagag
     1741 aaaaaacaag cgcgtcaatt agagaaagaa ttacgtcata aggaaaaagc attagcggaa
     1801 gccgctgctc tcttattact aagaaaaaaa gcacacgcga tttgggggga ccaagaggaa
     1861 gaatgatttc atcttcagat cgcaaacaag ctgttgaact tattcaagaa gccaatcgca
     1921 atggggcccg tctcacctat gcctgtaatg agctgaatat tagcgttcga acctacgaac
     1981 gatggacgcg tgaaggtacg atagcgcatg atcaacgccc tttggcgaaa cgtcctgtac
     2041 ccaaaaataa actgacggat caagaacgcg aaaaaataat agaaaccgtt tcaaaaaaag
     2101 aatttatgaa tttgccacct agccaaattg taccaaaact tgcggattgc tcgatttata
     2161 ttgcatcaga gtctagcttc tatcgtgtat tgcgagaaaa aaatatgcaa catcaccgtg
     2221 gccgaagtca agtttcacaa aaacgcatac cgccaagtca ccttgcgacg aagccaaatg
     2281 aagtttggac ttgggatatt acttggctaa aaggtccaat aaaaggactc ttttaccggt
     2341 tatatctcat cattgattta tttagccgta aaatggtagg ctgggagatt tgggaatcag
     2401 aagaagctcg tcatgccgaa aaacttatta aacagacggt gcttaaggaa cgtatccaag
     2461 gacagccgtt ggttttacac tccgataatg gcagccctat gaaagccgcg acttttcaag
     2521 tcttactcga aaaattagga attcaaagtt cttattcaag accacgagtg agtaatgaca
     2581 atccttattc ggaagcggct ttccgaacgt taaaatatcg accagaatat cccaataacg
     2641 gctttaaaac aatagatgat gctcgaaaat ggacgaaaga gtttgtcgat tggtataacg
     2701 attctcattt gcatagtggg attcaatttg tgtctcctgc agcgtgtcat gaaggacgac
     2761 atctcgctat tttagagaaa cgaaagcagg tttacgagga cgctaaaaag cgccatcctg
     2821 aacgctggtc aaaaggtgtt cgaaattgga ctccacatca atcggtggcg ttgaatcctt
     2881 taaaagaaac agaaagttaa caggagcata atctttcaag attatcaaat aaccctcgtc
     2941 acccaatagg ggaaaaagtt attcttcccc tattggataa cgaggccaac cgaccatagg
     3001 gaggttctgc tttttgatag gtggaaaata aaaatacgac aactatattg acaaacaccg
     3061 accatcgtcc agtaaagaga cgcaa
//
LOCUS       DANJLF010000152         3066 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000152 DANJLF010000000
VERSION     DANJLF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(22..1398)
                     /locus_tag="PWT25_002814"
     CDS             complement(22..1398)
                     /locus_tag="PWT25_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL1022814.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1526..2644)
                     /locus_tag="PWT25_002815"
     CDS             complement(1526..2644)
                     /locus_tag="PWT25_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1022815.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2786..>3066
                     /locus_tag="PWT25_002816"
     CDS             2786..>3066
                     /locus_tag="PWT25_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1022816.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
BASE COUNT          969 a          611 c          508 g          978 t
ORIGIN      
        1 ctttcataac caagtttcca attatcttct tggctcgtca tcttcgaatt cttttctgaa
       61 gtatcttcgt tcttcttcag gttccgcttc tgtttcttcc ggatcatgaa aaatgacacg
      121 gattttaagg attcgagaac cttccatttc ttcagaaacc agcgtaatat tatcgacatc
      181 aaacgataac ttttcgcctt catccggaat catgcctaat gctgtaatca aataaccagc
      241 catcgtatct acatcactca tatgcagatc actgtcaaaa gcttcattga actcatcaat
      301 cagcatacgt ccttggatga tatattcgtg ttcatcgatt ttttcataaa gattctcaac
      361 ttcatctgat tcatcatcga tttctccgac gatttcttct aaaaggtctt ctagtgttgc
      421 taacccaacg acgccgccat attcatctaa taaaatagcc atctgattct gcgtcttttt
      481 catttcatat aacagatcat caataaagac cgtttcagga acgaataaag gctcctgcat
      541 gattttcttg atatctaaat tatcaaagcc gaatttatgg gcagctttta gtaaattctt
      601 tgtatgaagg attccgacta ctttatcttt gtcttcgtta taaacaggga ttcttgaata
      661 gttttcggat aaaacttcat tcacattttc ttccacagtg tcgttgatat caatcataaa
      721 tgcatccgtt cgtgggacca tgacttctcg ggcaactttc gtatcaagag aaaaaacccc
      781 ttggagcatt tctaattctt cattattcaa tacgccttcg ttttcaagca tataacgcat
      841 ttcatctctg gtcatttttg aatcttcatc atcaaaagtc atcggcgtga tacgcgccaa
      901 taaacttgtg gaagcagaaa gcagccagac gaagggctta gctactacac caatcaagcg
      961 tatcagccct gaagtgaact ttgcgacttc ttctgattta tttaaagcaa tacgtttagg
     1021 gtacaattct ccaaaaacaa tcgatacata ggtcaaaata gccagcacaa tgatatttgc
     1081 aatatttttt gccgctgtgc cgccacctaa taccggagct aaccgttccg ataaagtatt
     1141 agctagtgaa gcaccagaca aaatgttgac caaagtgatt cctacttgga ttgtcgatag
     1201 gaaattattt ggattctgca atatttttaa aagcttttga gatttcacat ctccttcatc
     1261 agctttttgc tcgattcgat tcttattgat cgaaacaaca gcaatttcgg aagctgctaa
     1321 aaaagcattg atcaatgtca aaacgatcaa taataaaatc tgcgctaaca gcgactgact
     1381 ctcagggtca gcattcataa tcgcattctc tccttataaa attaattttt agtttacgat
     1441 agaaaaaaac gtggcactat agccacgtta aaatggtatc acaaagcaat tttctttgca
     1501 ataaaaacaa gtgtactaaa actacctacg aggtcgtagc aattttcttc ttgttttcat
     1561 tttgcttgtc ttctttgacg attttgatta tataggaaat gatcgtcttg cagattgcgt
     1621 aaaaaggaac acctaagaag ataccaagca atccggcaat atttccagct actagaagaa
     1681 cgataatgat cgttaatgga tggattttca gtgatttccc aattacatta ggataaatga
     1741 tattcccatc caactgttgg acgattagca caaccacaca acaaagtaat gcttttaccg
     1801 gatcattgaa gactgttact agaaatgcag gtgctagtcc tagataaggt cctaaatatg
     1861 gaattaggtt tgtcaatccc gcaatgaccc caaataagaa agcataatcc acaccaatta
     1921 ccatatatcc taaaaatgtg aaggtaccaa cgaataaaca ttcgattgct tgtccgctaa
     1981 tgtaattagc caatgtttta ttcagttgcc ccaataactc cacaatttgg tttcttcttt
     2041 tttctgggaa aaactgttgg atgtttggca ctagtttttc tccgtctttc aacatataga
     2101 acaagatgaa tggtgctgta atgattacta cggttgccga agcgatcgtt ccaacgatcg
     2161 aacctaaact attggatact ccactaagga attgctgaat gattgtcccg taagaaatgt
     2221 ctaattgctc aaaatattta ttgatatcta cttccttgaa caaaggcaat tcggctacct
     2281 cttttaacca gctttctacc tgtttgatga aatcaggcat attagatgcc aatgaggaaa
     2341 tttgctgtac tagacttggg atgacactaa gtaaaatcca aataattgct gcaactaaaa
     2401 ggagcagcac aattgcgaca gcccatattc ttttgatttt tgctttcatc aacagcttga
     2461 caataggatt caataaatag taaagaaagc ctgcaatcag tacaggtgca aataacgtgg
     2521 aaaaaaatgt cccgatcggt tcgaaaataa agtttatttc tgatgaaatc atgatcaatg
     2581 cagctatgat caataattca agcgaccaaa acattagttt agactgtcta agtttttcaa
     2641 acaatagatt tcctcctttc tttcttacta gtatatatta tattcgttaa aaattctttt
     2701 attctttttt tatttttgaa aacttgccat actttttatt tgttttattt tccagtatac
     2761 tatacgaaag gaaggtggct agaccatgaa agttgtacaa acaaaagata caatgagcaa
     2821 tatttactta gatgctgtac ggatcaggca tcaagttttt gtagtcgaac agggtgtgcc
     2881 tttgtctcga gaaatagaca aagatgaagc ccattgtatc cacttcgtcc tgtactcaga
     2941 taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta gaaaacggaa aaatgaaatt
     3001 acagcggatg gcgattcttt ctgaatacag acaccaaggg ttaggaaaaa tattgattga
     3061 agaagc
//
LOCUS       DANJLF010000153         3054 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000153 DANJLF010000000
VERSION     DANJLF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(1..354)
                     /locus_tag="PWT25_002817"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="PWT25_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="PWT25_002818"
     CDS             complement(614..1162)
                     /locus_tag="PWT25_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022817.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="PWT25_002819"
     CDS             complement(1163..2020)
                     /locus_tag="PWT25_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1022818.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="PWT25_002820"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="PWT25_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="PWT25_002821"
     CDS             complement(2582..2911)
                     /locus_tag="PWT25_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1022819.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          893 a          620 c          384 g         1157 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac ctta
//
LOCUS       DANJLF010000154         3012 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000154 DANJLF010000000
VERSION     DANJLF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            177..359
                     /locus_tag="PWT25_002822"
     CDS             177..359
                     /locus_tag="PWT25_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL1022820.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="PWT25_002823"
     CDS             462..746
                     /locus_tag="PWT25_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022821.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="PWT25_002824"
     CDS             762..998
                     /locus_tag="PWT25_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022822.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="PWT25_002825"
     CDS             1020..1274
                     /locus_tag="PWT25_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022823.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="PWT25_002826"
     CDS             1356..1541
                     /locus_tag="PWT25_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL1022824.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="PWT25_002827"
     CDS             1608..2003
                     /locus_tag="PWT25_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL1022825.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="PWT25_002828"
     CDS             1993..2370
                     /locus_tag="PWT25_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022826.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="PWT25_002829"
     CDS             2392..2766
                     /locus_tag="PWT25_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022827.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DANJLF010000155         2949 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000155 DANJLF010000000
VERSION     DANJLF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            384..1964
                     /locus_tag="PWT25_002830"
     CDS             384..1964
                     /locus_tag="PWT25_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL1022828.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2162..2385
                     /locus_tag="PWT25_002831"
                     /pseudo
     CDS             2162..2385
                     /locus_tag="PWT25_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(2590..2910)
                     /locus_tag="PWT25_002832"
     CDS             complement(2590..2910)
                     /locus_tag="PWT25_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL1022829.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT          998 a          484 c          615 g          852 t
ORIGIN      
        1 aaagacgtgt tgcttgaata aacaagcaac acgtcttttc ttttagtcag ctgacaaaaa
       61 aatatttgac tcttatggtt tggtggcttt ttcattcgct gaatccatgc tatactgatt
      121 cagttgtagt agttgacaaa ggttgtgaaa aagctatcct gcattaagta ataagagcag
      181 ccaagcaaaa acagcttttc gtatttttgg cttgggtggg cttattgctg ataggctgtc
      241 tctagaacgc cgtttactcg gaaaaagaga ctgctacacg gcttttgtca cagccctata
      301 tgaaattgtc tttctttaag agatcggaca atctgtgcct agagaaaata taaataattg
      361 aatggaagaa atggagttag gttatgaata atccaaaatt aaagcaacaa gtggacaatc
      421 gcagaacttt tgcgatcatt tcccatccgg atgctgggaa aacaacgatt acagaacaat
      481 tactattgtt tggtggggct atccgtcaag ccgggaccgt aaaaggcaaa aaaacaggta
      541 attttgccaa atcagactgg atggaaatcg agaaacaacg tgggatttct gttactagtt
      601 ctgttatgca gtttgattat caagggaaac gagtaaatat cttggataca ccaggacatg
      661 aagatttttc tgaagatact tatcgtactt tgatggccgt ggatagtgcg gtcatggtaa
      721 tcgatagtgc aaaagggatc gaagcacaga caaagaaatt attccaagtt gtaaaaaaac
      781 gtggcatccc gatatttact tttatcaaca aactagatcg tgatggtcgt gaaccattgg
      841 aattgttaga agaattagaa gagctattag atatcgaatc ttatccgatg aattggccaa
      901 tcggtatggg taaagggcta gaaggtcttt atgatatcta ccataatcgt gtagagtttt
      961 atcgtccgga aaattatcag gatgaacgat tagtagaatt ggatgaagat ggactgcttc
     1021 ccgaaaatca tcctctaact gaaaattcct tatatgaaca agtcttagaa gaagtagaac
     1081 taatcaaaga ggctggagat gcatttaatc aagaaaaaat tgctcgtggc gatcagacac
     1141 cagtattttt tggttctgca ttgactaact tcggtgtaca gactttttta gagactttcg
     1201 ttgaattggc accagctcct tatggacaca aaacacaaga tgatcagatg gtaagtcctt
     1261 atgaggaaga attttcaggt ttcgtattca agatccaagc aaatatgaat ccagcccatc
     1321 gtgaccgaat cgcttttgtc cgaatctgtt ctggaacatt cgaacgagga atggatgtct
     1381 ggttggaacg tacaaataaa aaactgaaat taagcaatgt cacacaattt atggcagatt
     1441 cgagagaaaa cgtcgaaaaa gcagtagcag gcgatatcat cggtgtatat gatacaggca
     1501 attatcagat tggggataca ttgttcgaag gaaaactaaa agttgcttat gaagaactcc
     1561 cttcatttac tccagagctg tttatgaaag tgactgcaaa aaatgtcatg aaacaaaaat
     1621 ccttccacaa aggaatctat caattagttc aagaaggagc gattcagctt tataaaacat
     1681 atctaacgga agaatatatc attggagctg ttggtcagct acaattcgaa gtattccaat
     1741 atcgtatgtt aaatgagtat aacgccgaag taatcatgtc tccaatggga cacaaaatcg
     1801 ctcgctggat cgatccagaa gatttggatg agaagatgag ttcaagtcgc aatatcttgg
     1861 ctcgtgaccg tttcgaccaa ccgttgttct tattcgagaa ccaatttgca gaacgctggt
     1921 ttgcggataa atatcccaat gtaaaattaa aaagtttgat gtaaagaagt cttgacacaa
     1981 gctgccctgc atcaagtaat aagaatagcc aaacaaaaac agcctctcat attttgttta
     2041 gagccgtgac ggattttccg tgacggctct ttttactata ttaaaatcat agaaatgtct
     2101 aatcaaaaaa atataaaaat tattttgcgt acatatgatt aaagaaagga gagtgtgaca
     2161 catggaaaca gaatggcaaa gacgtgaaag aaaaaaacag aaaaagaaga gatgttacga
     2221 ttatgtcgat tattagatgt aaaagactgg gacaaaaacg agcaaattgc acaaaaagta
     2281 cgatcaatca tccataaaga ggaaaaagtt aaaaataaag acacgcgtag aactgaaaat
     2341 gaaaagagaa aagtacaaaa gaaaagagaa aaatcaaaaa gctagaaaga aaaagaggct
     2401 gagacagaag cgtttaactc caagaaataa gctggaattc acaaaaattt gaaaagcaat
     2461 tttcgtgaat ttcagcttat ttccgaagga gttgcttctg ctttcgccgt ttatacgtgt
     2521 ttagagcgtg agacaaaagt gttctttact tttgtcccac gctctttaat tattatgttc
     2581 aaaatttgtt tacacggcta atgtagcgcc taattcatgt tttggaatcg agatttcaac
     2641 aaggcctgcc aattcgatta ctttgatttc atcctcgtgg taaacggcgt ctttgatttt
     2701 atctaagaaa taatttttag catcttcgat ttctttcggc tggattttac gtttgtcatt
     2761 gatcagcaga atttcattat gttcaggggc ttcagtgtag acaatagtgg tatttccggc
     2821 tgtgtagacc ttgactagat gggcatcagt gttttcaagc tggtttaata caagacggga
     2881 atggctgttt gttacattta ccaatttcat ggcgctcacc tcttttatgt atttttaatt
     2941 gcttgtttc
//
LOCUS       DANJLF010000156         2812 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000156 DANJLF010000000
VERSION     DANJLF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            384..572
                     /locus_tag="PWT25_002833"
     CDS             384..572
                     /locus_tag="PWT25_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL1022830.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="PWT25_002834"
     CDS             650..1663
                     /locus_tag="PWT25_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1022831.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="PWT25_002835"
     CDS             complement(1730..2764)
                     /locus_tag="PWT25_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1022832.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT          874 a          536 c          547 g          855 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gt
//
LOCUS       DANJLF010000157         2780 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000157 DANJLF010000000
VERSION     DANJLF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..985)
                     /locus_tag="PWT25_002836"
     CDS             complement(<1..985)
                     /locus_tag="PWT25_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDL1022833.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="PWT25_002837"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="PWT25_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL1022834.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..>2780
                     /locus_tag="PWT25_002838"
     CDS             1810..>2780
                     /locus_tag="PWT25_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1022835.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFE"
BASE COUNT          861 a          558 c          564 g          797 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa
//
LOCUS       DANJLF010000158         2739 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000158 DANJLF010000000
VERSION     DANJLF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="PWT25_002839"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="PWT25_002839"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL1022836.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DANJLF010000159         2531 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000159 DANJLF010000000
VERSION     DANJLF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..379)
                     /locus_tag="PWT25_002840"
     CDS             complement(<1..379)
                     /locus_tag="PWT25_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL1022837.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            598..1968
                     /locus_tag="PWT25_002841"
     CDS             598..1968
                     /locus_tag="PWT25_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HDL1022838.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1989..2273
                     /locus_tag="PWT25_002842"
     CDS             1989..2273
                     /locus_tag="PWT25_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022839.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          859 a          380 c          522 g          770 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac a
//
LOCUS       DANJLF010000160         2486 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000160 DANJLF010000000
VERSION     DANJLF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(243..464)
                     /locus_tag="PWT25_002843"
     CDS             complement(243..464)
                     /locus_tag="PWT25_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022840.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="PWT25_002844"
     CDS             complement(562..807)
                     /locus_tag="PWT25_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022841.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="PWT25_002845"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="PWT25_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..2408)
                     /locus_tag="PWT25_002846"
                     /pseudo
     CDS             complement(2031..2408)
                     /locus_tag="PWT25_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
BASE COUNT          850 a          429 c          348 g          859 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattggat gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata
     2461 aaaaacacac tggaggattt ttcatg
//
LOCUS       DANJLF010000161         2439 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000161 DANJLF010000000
VERSION     DANJLF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..189
                     /locus_tag="PWT25_002847"
     CDS             <1..189
                     /locus_tag="PWT25_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HDL1022842.1"
                     /translation="EKFYRLDNARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNYT
                     TFRVELPAMPDLVDKRRS"
     gene            404..1372
                     /locus_tag="PWT25_002848"
     CDS             404..1372
                     /locus_tag="PWT25_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL1022843.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1365..2396
                     /gene="vanA"
                     /locus_tag="PWT25_002849"
     CDS             1365..2396
                     /gene="vanA"
                     /locus_tag="PWT25_002849"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL1022844.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
BASE COUNT          719 a          457 c          606 g          657 t
ORIGIN      
        1 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
       61 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
      121 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
      181 aggtcctaag agatgtatat aattttttag gaaaatctca aggttatctt tactttttct
      241 taggaaatta acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc
      301 gtaagaacga gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc
      361 ccgttttatt tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat
      421 tactgtttat ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt
      481 tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc
      541 tttcaatcaa tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc
      601 gctgaagaga gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga
      661 tacaactgct gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag
      721 cgttgccgat tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt
      781 gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga
      841 catgacagtt ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg
      901 aggatttgga tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt
      961 accgtttgat gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac
     1021 ggatacgcac tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct
     1081 tatcaatact gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa
     1141 cgggaaactg ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc
     1201 tgattgcacc caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa
     1261 cgtgataatc acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga
     1321 aaaaaccatt aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag
     1381 ttgcaatact gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag
     1441 agatagccgc taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat
     1501 ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt
     1561 cagctgtact ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat
     1621 atgaaatcaa ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg
     1681 gatccataca aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa
     1741 gctcagcaat ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag
     1801 ctactcccgc cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct
     1861 atcctgtttt tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata
     1921 gcgcggacga attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa
     1981 ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt
     2041 tagttgttgg cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg
     2101 aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag
     2161 cagaggagcg aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta
     2221 gaggtctagc ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag
     2281 tcaatactct gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag
     2341 gtattgcact tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag
     2401 catggaaata ggatttactt ttttagatga aatagtaca
//
LOCUS       DANJLF010000162         2436 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000162 DANJLF010000000
VERSION     DANJLF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..272)
                     /locus_tag="PWT25_002850"
     CDS             complement(<1..272)
                     /locus_tag="PWT25_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022845.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="PWT25_002851"
     CDS             complement(309..446)
                     /locus_tag="PWT25_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL1022846.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="PWT25_002852"
     CDS             complement(448..894)
                     /locus_tag="PWT25_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022847.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="PWT25_002853"
     CDS             complement(891..1040)
                     /locus_tag="PWT25_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022848.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>2436)
                     /locus_tag="PWT25_002854"
     CDS             complement(1054..>2436)
                     /locus_tag="PWT25_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022849.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
BASE COUNT          638 a          502 c          420 g          876 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aagttaggct ggtacggtgt
     1981 ggctgttgag ccttcttcga ttttgatgtc gtacaactta aatccgccat ttattttatc
     2041 cctgtcaaca aagctaatac tcatataaaa tcgatctaaa tttgtgattt gataattaac
     2101 gttagctgta cctttgattg ttatttcttt ccctacatca tctgttgtaa tatttgtact
     2161 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2221 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2281 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2341 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     2401 gctcgatttt agtttggaca ttaaattcgg cttacc
//
LOCUS       DANJLF010000163         2407 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000163 DANJLF010000000
VERSION     DANJLF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            5..1150
                     /locus_tag="PWT25_002855"
     CDS             5..1150
                     /locus_tag="PWT25_002855"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL1022850.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="PWT25_002856"
     CDS             1321..1779
                     /locus_tag="PWT25_002856"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL1022851.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="PWT25_002857"
     CDS             1938..>2407
                     /locus_tag="PWT25_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL1022852.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DANJLF010000164         2400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000164 DANJLF010000000
VERSION     DANJLF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            207..2069
                     /locus_tag="PWT25_002858"
     CDS             207..2069
                     /locus_tag="PWT25_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1022853.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="PWT25_002859"
     CDS             2069..>2400
                     /locus_tag="PWT25_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1022854.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DANJLF010000165         2331 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000165 DANJLF010000000
VERSION     DANJLF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            319..936
                     /locus_tag="PWT25_002860"
     CDS             319..936
                     /locus_tag="PWT25_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1022855.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            977..1276
                     /locus_tag="PWT25_002861"
     CDS             977..1276
                     /locus_tag="PWT25_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL1022856.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1310..1477
                     /locus_tag="PWT25_002862"
     CDS             1310..1477
                     /locus_tag="PWT25_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1022857.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1522..2127)
                     /locus_tag="PWT25_002863"
     CDS             complement(1522..2127)
                     /locus_tag="PWT25_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1022858.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          789 a          369 c          432 g          741 t
ORIGIN      
        1 taaaaaaatt gtaaaagtta aagaagtcaa attctgatgt gaaaaataaa atataccatg
       61 gaagacataa aagaaaaact ggataactag tttatttaat ggcagaccgt taaggcctgc
      121 catttttatg tattttagcg gaataaatat aatcatgcgg gagtgtaaac ttactgtttc
      181 aaaaagtata ctttttgaca tgccttgatt ttctttgttt atgtatggca taataaccac
      241 ataatacaat ttgaaacgtt tctgatttaa tctgattgtt ttgaaacaat tttagtgaaa
      301 aaagggtaga taaaaacgat gagaaaattt ttttatatcc gggtcagtag ttatgatcaa
      361 tcgatcgaac gacaactgat ttccgcgaag gaattaggaa tccctgaaga atatatattt
      421 gtagaaaaag ccagcggcaa agattttaaa cgccctgaat atcaattaat gaaacggatg
      481 tttcgaaaag gagatttact ctatctacaa tcattagata gactaggcag aaataaacaa
      541 atgattttag aagaatggcg agaattaact aaggaaaagc agattgatat ttctgtgtta
      601 gacatgcctc tgttagatac tgctagttac aagcaactag atgggttaga gacgcttatc
      661 tctgacttaa ttctgcaatt actcagctat atggccgaag acgagcggaa acgaattaat
      721 agccggcaaa gagaaggcgt agaagctgcg aaacaaaagg gtgtaaaatt cggcagaaag
      781 aaaattgctt tacctcctga atttcctgcg atctattctg actggaaagc aggaaagata
      841 actgcagtct atgctatgga tcaattgggc atgaaacgaa atacctttta tcgacgggtg
      901 aaagaatatg aacatacctt gcaccacttt tcttgataga tataacagct attagacggg
      961 aagggagagt ttgaaaatgg acttaacgaa tcaaagggtc gtttttactg gaacgctgca
     1021 gcaaatgaca cgaacccaag ccatcggttt ggttcactct ttaaacggcc attgtcaatc
     1081 aagtgttact tcaaaaacaa atttcctggt ttgtggtcag tattctaagt cgttgtttga
     1141 tgattctctc tatacaaaaa aggaacaaac cgcgcgggac ttgattgcgt taggtcatga
     1201 gattaccatt ttatcagaag tcgaatttta tgcgttaatt agtcaacagt tgaaagaatg
     1261 gcaacggtat ttataatcgt tagtaaaatt tacaaggagg aaaattttta tgaagcgaaa
     1321 atcttatgat cgttggggca ttgaaataac tagttttaat cgaaatattg cttttcacgt
     1381 ggagtgtgac tgtggggaat tagcaaaaag tgtttatcaa aaaaattact tttactgtcc
     1441 taaatgccat cgccgatacg gggtacatgg aacatagaaa cgaaatagtg cggtagaaaa
     1501 gcgaatgaat gtagtatatt tctacaaatc attcgctttc tttattttat aaatagttgg
     1561 tcgagagatc ccgttttctt ttgcaatctc tgcaactgga atgccttgct ttaacatgtc
     1621 caccacggtt ttaaaaacta agcgtttttg cggatctgtt gagttaatag aatactcgat
     1681 tggtcgtccc ttatatcggc cggctttttg agccaataat actccttgcc gctgacgttc
     1741 acgaattttc tcacgctcgt tttgtgcaat ataacttagc agtgataaaa acatatcgaa
     1801 catagcggta tccaggagtt catttccagt attaaaatct aaaaactgtg catctaatat
     1861 ttttaacgct acttttttct gtttcataaa agcaactgta ttttttatat cttcataatc
     1921 tcttcctaaa cgatctagtg atgtaacgat gacgcaatca ccttcacgga tataatttag
     1981 taatttttga aactcaggac gctcagtatc tttaccactg gctttttctt ggaataattt
     2041 atcaatgtta agtccttcca tcattgccaa ttgtcgatcc aaattttgat cttgtgaaga
     2101 tactcgaata tagccaattt ttcccataaa aaactccttt ttaaaagtga tataatttac
     2161 atgtaaaatt tacttgtagt tttactgcta tcatagcatt tttaaaaata aattttatgt
     2221 agacttaaaa tttacttcat ggagggttta aatggatgaa atcaaaagta acaaaaaagc
     2281 atttggcatt tatttaggag aacctagtac agaacaatta aaccaatttt t
//
LOCUS       DANJLF010000166         2233 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000166 DANJLF010000000
VERSION     DANJLF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..544
                     /locus_tag="PWT25_002864"
     CDS             <1..544
                     /locus_tag="PWT25_002864"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL1022859.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="PWT25_002865"
     CDS             complement(900..1472)
                     /locus_tag="PWT25_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1022860.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="PWT25_002866"
     CDS             complement(1488..2093)
                     /locus_tag="PWT25_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL1022861.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt cta
//
LOCUS       DANJLF010000167         2228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000167 DANJLF010000000
VERSION     DANJLF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(24..2108)
                     /gene="cadA"
                     /locus_tag="PWT25_002867"
     CDS             complement(24..2108)
                     /gene="cadA"
                     /locus_tag="PWT25_002867"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL1022862.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          640 a          508 c          354 g          726 t
ORIGIN      
        1 aggatataaa aaaaagagga cgttcattcg tcctcttttt ttacatgcaa gcttcgcatg
       61 gcattcaaaa cagctaatac tgtcacccca acatctgcaa atactgcatc tcccattgat
      121 gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta tcccaatagc aaagacaatg
      181 ttttgtttga cgattttcaa tgttttcttt gctaaatgga ttgcttctgc aatcttcgat
      241 ggttgatcat tcatgatgac tacatctgct gcttcaattg cagcatcgct tcccaatcca
      301 cccattgcga ttcctacatc tgctcttgct agcaccggtg catcgttgat tccatctccg
      361 acaaatgcgg tctttttatt gtttctttgt aagatttcct ctaaccgctg cactttatct
      421 tcaggaagca attcgctata tatcttgtcc acgcctactt gcttcccgat atgatcagca
      481 atttttttcg agtcgcccgt caacataact gtgttcttca caccagcctg ttttaattga
      541 accaatgcgt ctacggcatc ttctttcaag gtatctgcaa taacaagata accactgtaa
      601 ctttgatcga tcgctagata aagcaatgta ccgatttctt gacttaaagg aaaagagatt
      661 tcaaatcttt ccatcaattt gtgattacct gctaatactt gtttgccatc aacttcagct
      721 aagatccctt cacctgcaaa ttcctggata ttagttgttg gtaaaagcgg acctgcgtag
      781 ccttctaaaa cagattgagc aatcggatgt gtagaaaact gttctgcact cgcaatatat
      841 tgcatgaatt tctttggatc gatttttgta tcgatcgtct ggatcgaaaa gtcacctttc
      901 gttaatgtgc cggttttatc gaataccact gtctcggttt gcgcaagcaa ctccaagtaa
      961 tttgaacctt ttactaatac accgatttta cttgcgcctc cgataccgcc aaagaaacta
     1021 agtggcacgg aaatcactaa tgcacatgga catgagatca ctaaaaatgt caatgctcga
     1081 tagatccatt cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac agctaataaa
     1141 acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat gaaattttca
     1201 gctggtgctt ttttactgct ggcattttct actaattcta ataatttgct gatagtagaa
     1261 ttttcataag atttttctac ttgaactaaa aatggtttat ttttattgat aaatccgctt
     1321 aatactgttt caccaacata tacagattta gggactgatt ctcctgtaag tgctgacgta
     1381 tcgactaatg attctccttc gatgatcgtt ccatctaacg gtacacgttc accaggcttc
     1441 acaagtacaa actgaccgat ctgcacgtct tcagggttca cggtcatcaa tccttccgtc
     1501 gtctgcacgt gagctgtttc tggacggatc gccatcaatt cgcgaatcga ttttcttgaa
     1561 tgactgactg ccaatccttg gaaatactcg ccgatttgat aaaatagcat cactgctacg
     1621 gcttctggat attcaccgat caacatagcg ccaatcgtag caacagacat aagaaaattc
     1681 tcatcaaaga tttttccttt gacgatattc atcactgcct tcttcactac atcatatcct
     1741 atcagcagat aaatcaataa ataccctatc aagcgaattg gttcattaaa atcaaataat
     1801 agcaataaag cgatacctgc aagggaaaat aaaatccgga acagcgtatt tctctctttt
     1861 tcatttccat gttcagatac tttttcctta tctatttctg ttactttgac atctggttct
     1921 aattccttca ctactttttt tgtttcttgt tcaatcgtag ccaaatgatc ttcctctgcg
     1981 tcaatcgtta gttttccaga agtgaagttt accgttgctt ccttgactcc attgatcttc
     2041 tgaactcctt tttcgatttt cattgcacag ttcgcacaat ctaaaccttc taatctgtaa
     2101 cttttcattt taatgacctc ctcgcctcaa catatgaaca tctcttcata actaattata
     2161 aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac atctgcatcg
     2221 gaaaattt
//
LOCUS       DANJLF010000168         2065 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000168 DANJLF010000000
VERSION     DANJLF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..1008
                     /locus_tag="PWT25_002868"
     CDS             <1..1008
                     /locus_tag="PWT25_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022863.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="PWT25_002869"
     CDS             1025..1174
                     /locus_tag="PWT25_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022864.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="PWT25_002870"
     CDS             1171..1617
                     /locus_tag="PWT25_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022865.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="PWT25_002871"
     CDS             1619..1756
                     /locus_tag="PWT25_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL1022866.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="PWT25_002872"
     CDS             1794..>2065
                     /locus_tag="PWT25_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022867.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DANJLF010000169         2058 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000169 DANJLF010000000
VERSION     DANJLF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..337)
                     /locus_tag="PWT25_002873"
     CDS             complement(<1..337)
                     /locus_tag="PWT25_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL1022868.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="PWT25_002874"
     CDS             complement(404..559)
                     /locus_tag="PWT25_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1022869.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="PWT25_002875"
     CDS             1176..1397
                     /locus_tag="PWT25_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022870.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="PWT25_002876"
     CDS             1398..1655
                     /locus_tag="PWT25_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022871.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="PWT25_002877"
     CDS             complement(1791..>2058)
                     /locus_tag="PWT25_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL1022872.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DANJLF010000170         2003 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000170 DANJLF010000000
VERSION     DANJLF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..378
                     /locus_tag="PWT25_002878"
     CDS             <1..378
                     /locus_tag="PWT25_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL1022873.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="PWT25_002879"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="PWT25_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL1022874.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="PWT25_002880"
     CDS             1859..>2003
                     /locus_tag="PWT25_002880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022875.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DANJLF010000171         2000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000171 DANJLF010000000
VERSION     DANJLF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..914)
                     /locus_tag="PWT25_002881"
     CDS             complement(<1..914)
                     /locus_tag="PWT25_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL1022876.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="PWT25_002882"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="PWT25_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL1022877.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          659 a          367 c          392 g          582 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca
//
LOCUS       DANJLF010000172         1993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000172 DANJLF010000000
VERSION     DANJLF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="PWT25_002883"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="PWT25_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL1022878.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="PWT25_002884"
     CDS             853..1041
                     /locus_tag="PWT25_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL1022879.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="PWT25_002885"
     CDS             1054..1611
                     /locus_tag="PWT25_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1022880.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="PWT25_002886"
     CDS             1643..1885
                     /locus_tag="PWT25_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1022881.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          725 a          308 c          406 g          554 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctttatt cct
//
LOCUS       DANJLF010000173         1936 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000173 DANJLF010000000
VERSION     DANJLF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            84..1550
                     /locus_tag="PWT25_002887"
     CDS             84..1550
                     /locus_tag="PWT25_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1022882.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          597 a          331 c          396 g          612 t
ORIGIN      
        1 aaataacact gtcttgtaag tttgttaaaa attgaatatg attacatctg gataacaaat
       61 ttttatcata aaggagagaa cgaatgaaca acaaacaatt acgctggttt actgttgcgt
      121 taattgcatt taacatggtt tggggcatgg ggaatgtagt taataactat gctcagcaag
      181 gagtatcagt tgtcacctct tggctattga ttttggccat ttattttatt ccatacgcat
      241 tgatcgttgg acaattagga tcagccttca aggatagtaa aggaggcgtc agttcatggg
      301 tcgaaaatac gacatctaat aaacgactgg catactatgc cgcttggact tactgggtcg
      361 tccatattcc ttatttggca caaaagcctc aagcaattct gattgctggt ggttgggcag
      421 tccaaggaaa tggaaatatc gtaaatacga tgtcagtaca aatggtagca gttatttcac
      481 ttattatatt tcttgccttt ttatatttat caacaaaagg actttctact ttgaaagtga
      541 ttggcggact ggccggaaca gcgatgttcg tcatgtcgat cttatttatt cttttagctg
      601 taacggcacc gttcatcaat ccaacgatgg aatttgccac accacatatg aatcaagtga
      661 aaacatatat tccaacattt gatttcagtt atttcacaac cgtttcgatg ctagtatttg
      721 cagttggagg ggcggaaaaa atatcccctt atgtcaactc tacaagaaat cctgcaaagg
      781 aatttcctaa agggatgatc ttcttagcag caatggtagg gatttgtgct gtattaggct
      841 cagtcgcaat gggtatgctg tttgctagtg ataatattcc taaagacttg atggcaaatg
      901 gggcatacag tgcttttcaa attttaggta attactatgg tatcggtaac tcattgatga
      961 tcatttacgc attgacaaac atgattggac aaatctccgc attagccttt tcaattgatg
     1021 caccactaaa aatcttgtta gcagatgcgg atccagaatt tgtaccaaac tggctacgta
     1081 aacgtaatcg taaaggtaca ttggtgaatg gttatcttct gacaggtata ctagtaggaa
     1141 tcattatttt gttgccgatt tttggtatca aagatatgaa tgaattagta aaatggttga
     1201 cgaacttgaa ctcagttgtc atgccaatgc gttacctatg ggtgttcttg gcatatatga
     1261 tgttgaataa agcttataag aaattcaatt ctgaatataa atttgttaaa aatccaaaaa
     1321 tcggttttat ctttggtgct tggtgctttg ccttcactgc gtttgcatgt atcctaggaa
     1381 tggtgccaaa agtagattat actgctgatc ctaaagcatg gtggttccaa ttatttacga
     1441 atatcatcac accaatcgtc ttgatcttat taggtatgat cttgccagca attgctcgtc
     1501 gagacaaaaa caaaaaaatc actgaaccag aaacagtggt tcagccttaa aatagaaata
     1561 cttgttacaa gaggttgtga cagaagtggt cagcttcaag aaataagaag gaatttacga
     1621 aaattgcttc tcaaattttt gtgaagtttc ggcttgtttt tcgaagaagc tacttctgga
     1681 acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
     1741 caaaaatagc ccctcatatt tttcgtttgg ctgggcttat taccgcagat gcaagctttg
     1801 gaacaccgtt taaggaagag gttgtaaaat cagcgatttg acctgagtat tagagaaaca
     1861 aacggaaaaa tagctcctca tattttgccg ctttttgagc taatgctgaa ggtcagctga
     1921 ttgaacacca tttatt
//
LOCUS       DANJLF010000174         1796 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000174 DANJLF010000000
VERSION     DANJLF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="PWT25_002888"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="PWT25_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL1022883.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DANJLF010000175         1790 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000175 DANJLF010000000
VERSION     DANJLF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..162)
                     /locus_tag="PWT25_002889"
     CDS             complement(<1..162)
                     /locus_tag="PWT25_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL1022884.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEY"
     gene            371..583
                     /locus_tag="PWT25_002890"
     CDS             371..583
                     /locus_tag="PWT25_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022885.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            745..1263
                     /locus_tag="PWT25_002891"
     CDS             745..1263
                     /locus_tag="PWT25_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDL1022886.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1270..1782
                     /locus_tag="PWT25_002892"
     CDS             1270..1782
                     /locus_tag="PWT25_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022887.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          686 a          233 c          273 g          598 t
ORIGIN      
        1 atattcttgc acccaacgat aaatcgtcgt atgagaaacg ttaatgccac gatcatataa
       61 gatttcttga acttcacgat agctaaggtt ataacgaaga tagtagccca cggctacaat
      121 aatcacatcc tgctgaaatt gctttccttt aaaatgattc atcgtcattc ctcctgctat
      181 ctttttctat tattctacct tatttgatag tagatttaaa actttgcaac agaaccctaa
      241 aaacactata tattccgaaa agtgagtatc tccattcata aaataatcaa catatttaaa
      301 aaatctaatt agctttttta gtcaatctaa agttctttat tctatgatat aatgttactg
      361 tagaaagttt gtggcggttt ggcaaatcca attgaaggag cgtggtgcct atgggcttat
      421 tagcttatcg gacgtactct gtagaaagga aatgcctgat ttgttctcat tggatcaaat
      481 cattgctatt atcggagcag cagcagtagt tattactgcc gtagcgaaag ctagcgattc
      541 gattttagct tggttaaagt tcaaaaaaga acaaaaaaaa tagagccgtc tcaacttttg
      601 gcgaggtaga cgagctctaa agctttattt acatgccatc cgctttgaac ggactacact
      661 gggcatgtga cagcatgccc tttcatttat attatatctc attcttttat ataatgaaag
      721 taaataaatc atggaggata aaatatggat aaatttaata gattaacatt aataaatcag
      781 tttgaaattt taaaagcttt aaatcctaac gaagaaaaat actatgctga aaaaatagaa
      841 atactaacag aaggttacga atatcactat agtgaaatat ttgaaaatat ttctgatcca
      901 ttgccagaag aagattcgcg gtttgttcta gacatattaa atatgtatag agatattact
      961 tttagtaaaa ataaattaaa agatataaat tcaattgata attattatat acaatttaaa
     1021 ggttttgact ttaatagctc taccgaattt aagttagcac tttatgcaca attttttatt
     1081 gaaaaattaa ataggtttca agaaatagtt gaagatagtg attttaacac ttttaatagc
     1141 cataaaccga tgagaggtac ttatataaaa tttctcgaaa attataatga tttaaaatct
     1201 accaataatt atcaatttgg aaacttgtct tatgagatga taagtaaagt tctaagtttg
     1261 tgatgataaa tgaaattaaa tagtataaat gatttatttg ctattatagg tgttattgct
     1321 aacatacttt tttcgtgttt gaattttaga tttactcaaa agaacaaaaa gaacaaactc
     1381 agtatagaac aaatgaaaaa agacattgaa actgatttaa aaaatgacca taaagaaaga
     1441 agaaaaattg aagaatatac ttctttaatg gaaaagtgta aagcaactga tgcaattttt
     1501 agctttttaa gaaaaagtga tgagataatc acacgtgaaa atatagattt agaaaatgag
     1561 attagtaaac ttgaatctat cttttccgat ataaattctc ttaagtattt aatcgtcgaa
     1621 gaaaattcgg atagcttttt agaatttgaa aaatatctaa cagatcttat agataataga
     1681 aataatgaat taatgagaca aaacattgca agattcctta ctaaattagt ttctgaatgg
     1741 aaaagtatta tttctcaaaa tatggataaa ttaagatatt aaggatgtta
//
LOCUS       DANJLF010000176         1740 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000176 DANJLF010000000
VERSION     DANJLF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            87..1115
                     /locus_tag="PWT25_002893"
     CDS             87..1115
                     /locus_tag="PWT25_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL1022888.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1171..1389
                     /locus_tag="PWT25_002894"
     CDS             1171..1389
                     /locus_tag="PWT25_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022889.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1460..>1740
                     /locus_tag="PWT25_002895"
     CDS             1460..>1740
                     /locus_tag="PWT25_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022890.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNI"
BASE COUNT          658 a          200 c          321 g          561 t
ORIGIN      
        1 tataattata actcatgatc acattctaga taattttttg gattcaaatg atgaaatatt
       61 atatttaaat aaggagtatg ataataatga atagcaaaaa acttcagatg ggtgaaattg
      121 ttagaggtcc tagtcaaata tctattgatg ttacatataa atgtaatttt agatgtaaac
      181 attgttacaa tgcgagtggt ggagacaatt gttttatgga taatgagttg actgatgatg
      241 aactgttaga acttacaaaa gatatatgta atttaaagcc atataatatt tgtttctgtg
      301 gaggagaaac tttactgaga aaaaaaatag ttttatcttg tgccacttta ttaaaagagg
      361 caggtattcc taatattgct attgttacta atggatcatt agtaactcag gatgttgcaa
      421 aagaattatt caaagcaggt attaatcgat ttcaagtaag tttagatgga aaaaatgcta
      481 gtagccatga agcacttaga ttatatccaa acagttttga aaaggcaatt aatgctatca
      541 aattatttag agaattgtcc gaagaagtgg atgttgcttt ttgtcctact agttttaatt
      601 acaatcaatt acgagaagta tatcaactag taaatactct taaagtgaag tctttaagaa
      661 ttcaaccatt tatgcttagt ggtaggggaa tgcaaaatag ggaaatagtg ccaactgttt
      721 ctcaatatag aaaactagtt tctgatatta actatattaa aagaaatgaa gattatacca
      781 agatagattg gggagatcca attgatcata tcatccgaca cagaacttta ttgaaagatt
      841 tttcgaattt tgtttctatt aggtcagacg gaggattaat ggcatcacct tacttgccaa
      901 ttcaagttgg aaatattaaa tgtcacagtc tttctaaata ttggcgagca ggactttata
      961 aagttttttc tttagattta gttcaagata tagctactca agtttcttca atacaagata
     1021 tgagtaaaga attaaaaaaa ggacatatta aatggcttaa tgaagatata gaaattgata
     1081 ttctcgatga atacaaacat gatattttaa tttaggttta tcattggaaa gtttagataa
     1141 acaaagtact tttagaaagg agggaaaaaa atgatttctg aacctcaaat gaataatatg
     1201 tatgaaagcc caactataat gggagctggt gaagctgcgc caagagcttg ttcagcaatt
     1261 atggtgtgtg tgttattagc atctgcagga gtaggtatcc aagcaggagc ggtgtatact
     1321 tatgctgtag ttgctacagt agctgtagct gcagccgcag tttggaaaac agtaggatta
     1381 gttggttagg aattaatagg agactaactg tctcctattg attccaatga ttaagctaag
     1441 gaggaatttt aagaaaataa tgaaatacaa taatttaaaa ccgaaattac ttaatgagaa
     1501 catagaatat ataagagaag aaaattatgg atatttaacc atagttccca aaaatggaac
     1561 tgaattgaaa gataacgttt tcaatccaac agtaagatat atattggcta actgtactgg
     1621 aaaaaacagt gtaaaaaaaa tagcgaatgg tttaaaaaag aagtttccta gtttagaata
     1681 tgaaaaattc tatcaagatg ttgtaggaac tattaaattt ttcaaacact ataatattat
//
LOCUS       DANJLF010000177         1679 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000177 DANJLF010000000
VERSION     DANJLF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..1373
                     /locus_tag="PWT25_002896"
     CDS             <1..1373
                     /locus_tag="PWT25_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1022891.1"
                     /translation="VDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTY
                     PDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVT
                     DVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1507..>1679
                     /locus_tag="PWT25_002897"
     CDS             1507..>1679
                     /locus_tag="PWT25_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1022892.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLE"
BASE COUNT          664 a          249 c          357 g          409 t
ORIGIN      
        1 cagtagatct aacagataat gttactaact tgccagcgtt accagaagga acaacagtaa
       61 cagatgttac tcccggcggt acaattgata ccaacacacc aggtaattac gaaggtgtca
      121 ttgaagtaac gtatccagac ggtacgaaag atacggtgaa agttccagta gaagtgacag
      181 acaatcgttc tgacgccgat aaatatgaac caacagtaga aggcgaaaaa gtagaaattg
      241 gaggcacagt agatttaaca gataatgtta ctaacttgcc agcgttacca gaaggaacaa
      301 cagtaacaga tgttactccc ggcggtacaa ttgataccaa cacaccaggc aattatgaag
      361 gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt ccagtagaag
      421 tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa gtgactacag
      481 acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat ttaccaaaag
      541 gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat aaaaaaggta
      601 ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac gttacagtga
      661 cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg actacagact
      721 taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta ccaaaaggaa
      781 ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa aaaggtactg
      841 tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt tctgtagtag
      901 ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac caaattgtta
      961 ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga agtcaacacc
     1021 atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta gaggcaggga
     1081 caaaagtgat agtaactgca actgatgaat ctggtaatac tagtgaacct actaatgtag
     1141 ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt aagactgaca
     1201 atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc tcaaaagttt
     1261 ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt ttaatactgt
     1321 taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa tagaaaatca
     1381 atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt tgctttttta
     1441 tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg aaaagaatgt
     1501 aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc cgcagctcga
     1561 tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa atcagaaatt
     1621 agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa tttagaaga
//
LOCUS       DANJLF010000178         1664 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000178 DANJLF010000000
VERSION     DANJLF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            131..505
                     /locus_tag="PWT25_002898"
     CDS             131..505
                     /locus_tag="PWT25_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL1022893.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PWT25_002899"
     CDS             complement(619..1248)
                     /locus_tag="PWT25_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1022894.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PWT25_002900"
     tRNA            complement(1382..1454)
                     /locus_tag="PWT25_002900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DANJLF010000179         1573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000179 DANJLF010000000
VERSION     DANJLF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DANJLF010000180         1509 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000180 DANJLF010000000
VERSION     DANJLF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            101..868
                     /locus_tag="PWT25_002901"
     CDS             101..868
                     /locus_tag="PWT25_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1022895.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="PWT25_002902"
     CDS             1060..1458
                     /locus_tag="PWT25_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL1022896.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DANJLF010000181         1492 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000181 DANJLF010000000
VERSION     DANJLF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(22..312)
                     /locus_tag="PWT25_002903"
     CDS             complement(22..312)
                     /locus_tag="PWT25_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022897.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(520..1212)
                     /locus_tag="PWT25_002904"
     CDS             complement(520..1212)
                     /locus_tag="PWT25_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL1022898.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          401 a          274 c          211 g          606 t
ORIGIN      
        1 tttgtctcag ccgcttgttt tctagtttat tctttcatct tgtcggacct tttttcttgg
       61 gtatagactt ttctcatatt ctaccacaat tttgggtttc cctaacacat gataggatct
      121 cacaacaaat tgctttctat tattataata acctgtaacg actaacttca tcccttcgtc
      181 tacttcagct aggaagttca aactatggga agcaatcaaa caattgaccc catttaaact
      241 gaaacggatc aacggtctgg cactaacttg caaagtacgg atcttatcta ctgttccata
      301 ttttttctcc ataaccttca cctcaatacc attatacgaa catacattcc ctttgtaaag
      361 aagaaactct gttttatctt tttgactttt tgtctattac ctttcttaaa atcatattgg
      421 cacttcctgc gaagatgaag gacatgtact aaaaaaaaga ggcagcagta tagattactg
      481 ttgcttcttt actaatttag gtttacttgt taagaaagat tatttacgca aatgttcttc
      541 aacaatttgc tgagatttat cagctgtatc ttccacttca tcttttgctt tttctaactt
      601 ttgaatatat tgtttcaagc ttgagttttc agcagttgct tcgtctaatt tcttttgtgt
      661 atctgttaac tgagtttcta catttgtttt ttcttgtgtc agtgttgcga tttgttgatt
      721 caaaccattt acagtatttt gtaattctga aagttttgct tgtgcttctt gtagtcttgc
      781 tgtccaatct gcattcagat gattgatttc atcatttttt tggttgatct catctagttt
      841 gacattgatt tcattttgtt tgctttgtag ttgtgcatcc ttgctgacta attgactttc
      901 taagttttgt ttttctgctg ccgattgatt tttttggttt tctaattcat ttacttttgt
      961 ttggtattct tggacatctg cctttgaatc ttgcaatgtc tgttcttttt gtgctagtcg
     1021 agcggtaaga tctgtaagcg cttgctcttt atctgtcaat gtatgatcaa gctgtgtcag
     1081 attctcattg attgtatcaa tatctgtctc tccaccccac aaaataattt catttgcgta
     1141 ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg acaatcccac cagtaataaa
     1201 tttctttttc atttgcatag ctccttaatc caatactttt acatgctaaa agtaagtgat
     1261 ctcaaacaca atgtcaagcg tttttaaatt acatgtgtaa ttttaatata aatactatca
     1321 aatactcatt taatcctcct tttttatcta tttaaaaaat cttttttata gagaaaaatg
     1381 caggttaata ctttcttaaa taaatattta tggtgaacaa aaacaaaaaa gcaagtttta
     1441 ttaaacgtaa cccttttcat ttcatagatt actgtattta ttacaaaaaa cg
//
LOCUS       DANJLF010000182         1485 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000182 DANJLF010000000
VERSION     DANJLF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            287..694
                     /locus_tag="PWT25_002905"
     CDS             287..694
                     /locus_tag="PWT25_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022899.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="PWT25_002906"
     CDS             708..1109
                     /locus_tag="PWT25_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022900.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="PWT25_002907"
     CDS             1111..1482
                     /locus_tag="PWT25_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022901.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          570 a          183 c          259 g          473 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatga
//
LOCUS       DANJLF010000183         1465 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000183 DANJLF010000000
VERSION     DANJLF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..626)
                     /locus_tag="PWT25_002908"
     CDS             complement(<1..626)
                     /locus_tag="PWT25_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1022902.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1465
                     /locus_tag="PWT25_002909"
     CDS             914..>1465
                     /locus_tag="PWT25_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1022903.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPY"
BASE COUNT          506 a          215 c          279 g          465 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaaactc cttat
//
LOCUS       DANJLF010000184         1451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000184 DANJLF010000000
VERSION     DANJLF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..625)
                     /locus_tag="PWT25_002910"
     CDS             complement(<1..625)
                     /locus_tag="PWT25_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL1022904.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="PWT25_002911"
     CDS             complement(784..1050)
                     /locus_tag="PWT25_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1022905.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="PWT25_002912"
     CDS             complement(1040..1396)
                     /locus_tag="PWT25_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1022906.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DANJLF010000185         1447 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000185 DANJLF010000000
VERSION     DANJLF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(943..1356)
                     /locus_tag="PWT25_002913"
     CDS             complement(943..1356)
                     /locus_tag="PWT25_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL1022907.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          531 a          238 c          214 g          464 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctt
//
LOCUS       DANJLF010000186         1437 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000186 DANJLF010000000
VERSION     DANJLF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            105..1406
                     /locus_tag="PWT25_002914"
     CDS             105..1406
                     /locus_tag="PWT25_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL1022908.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          288 c          264 g          412 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttct
//
LOCUS       DANJLF010000187         1417 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000187 DANJLF010000000
VERSION     DANJLF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(150..1337)
                     /locus_tag="PWT25_002915"
     CDS             complement(150..1337)
                     /locus_tag="PWT25_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL1022909.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DANJLF010000188         1404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000188 DANJLF010000000
VERSION     DANJLF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..969
                     /locus_tag="PWT25_002916"
     CDS             <1..969
                     /locus_tag="PWT25_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1022910.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="PWT25_002917"
     tRNA            1159..1232
                     /locus_tag="PWT25_002917"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          457 a          246 c          280 g          421 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tact
//
LOCUS       DANJLF010000189         1373 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000189 DANJLF010000000
VERSION     DANJLF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            191..445
                     /locus_tag="PWT25_002918"
     CDS             191..445
                     /locus_tag="PWT25_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1022911.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="PWT25_002919"
     CDS             446..763
                     /locus_tag="PWT25_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1022912.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DANJLF010000190         1355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000190 DANJLF010000000
VERSION     DANJLF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..1355
                     /locus_tag="PWT25_002920"
     rRNA            <1..1355
                     /locus_tag="PWT25_002920"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DANJLF010000191         1350 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000191 DANJLF010000000
VERSION     DANJLF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            76..>1350
                     /locus_tag="PWT25_002921"
     CDS             76..>1350
                     /locus_tag="PWT25_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL1022913.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          459 a          261 c          257 g          373 t
ORIGIN      
        1 ggtattaaaa aacatatcgt tctctagtat gattaaatca ccacaaaata aacaactgag
       61 aggacgatac gctatatgaa tgattctatc aaaaaaatgc tgagaataac agagaaagat
      121 ttgatgatta cagaggtctc ttacgagacc cttcagaaga aaaagacgtt ggtcgtcgat
      181 gctgttctct cgcctactcc tcgtgcttgt agaagttgtg gttctactgt ggtagatgga
      241 aacgggaaag caattatagt gaaaaatgga aaaaaggaaa cgattgtccg ttttgaacaa
      301 tacaatcata tgcctttggt tatgcgccta aaaaagcaac gctatacctg taaaaactgt
      361 cgcacccatt ggacggctca aagttatttt gtccaaccca gacattcaat cgcaaatcat
      421 gttagatata aaattgcttc tttactgact gaaaaagtat ctttatcttt tattgcgaaa
      481 agctgtcagg tatctttgac caccgttatt cgtacattga aagagtttaa aagctattta
      541 ccaaagcaat ctaagaagat cctccccaga gtattgatgg ttgacgaatt tcgttcgcat
      601 gcttccatag aagataagat gagctttatt tgcgcggatg gcgaaacagg aaaattaata
      661 gatgttttgc ctacgcgtaa attacctcga ttaacaagct atttcttagg ttgtaccaat
      721 ccagaagaag tagaattctt ggtgacagac atgaacgccg cctacttcca gctcaccaaa
      781 cgtgttctgc caaatgcgaa agtcgtgatt gatcggtttc atattgtcaa acacatgaat
      841 caagcgttca atgagttgcg tatccgtgaa atgaatgaac ttcgtaaagc cggacagaaa
      901 agccaggcag aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc
      961 aaccattatg aatacaaaac atggaaaagt ttccgagcac caaaataccc atttcttact
     1021 gaagcaatga tgattgatcg attgcttgag ttttctccgc ccctaaagga ggcgtatccc
     1081 ttttttcatg aattagttga agcctttcga gacaaagacc ctgacttatt tttctcctta
     1141 ttggcagaac ttcccgaaac gttagatgac agctttcggg aaaagcttca aaaccttctg
     1201 acctatgaag aaggcatcac caacgcaatg atctatcctt attccaatgg aaaaatagaa
     1261 gcgaagaata cccacataaa gacaatgaaa cgagtatcct acggatttaa atcatttgag
     1321 aacatgagaa ttagaatctt tttgatcaat
//
LOCUS       DANJLF010000192         1324 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000192 DANJLF010000000
VERSION     DANJLF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            278..820
                     /locus_tag="PWT25_002922"
     CDS             278..820
                     /locus_tag="PWT25_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022914.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="PWT25_002923"
     CDS             976..>1324
                     /locus_tag="PWT25_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL1022915.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DANJLF010000193         1320 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000193 DANJLF010000000
VERSION     DANJLF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            39..656
                     /locus_tag="PWT25_002924"
     CDS             39..656
                     /locus_tag="PWT25_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022916.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          495 a          174 c          210 g          441 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
//
LOCUS       DANJLF010000194         1306 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000194 DANJLF010000000
VERSION     DANJLF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            79..780
                     /locus_tag="PWT25_002925"
     CDS             79..780
                     /locus_tag="PWT25_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL1022917.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          458 a          233 c          234 g          381 t
ORIGIN      
        1 gttggtatgt taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa
       61 taaggagagg actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga
      121 tatcaacctg attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact
      181 gttcttcgtt cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg
      241 ttgtatatct tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat
      301 tactactatg actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa
      361 gagacctaca cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt
      421 tcctactatg aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa
      481 atttctaaag tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc
      541 tttgaccggg taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa
      601 gatggtcata tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc
      661 acgacacgtg aaactgctgg acaagtaatc aaacaactac gtgattccgg gctgttagat
      721 tatgacaaaa agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa
      781 cgcgaacaaa aataagcagt gaccctgata aatatcaggg tcactgctta ttttcctccc
      841 taaaacttct gaaagatccg gcagttttag atcactggac aagactcaaa gattttctat
      901 gtgtgtttca gttatgtaat attcactgtt tgcaaaaaat actgttagat taactagatg
      961 aagcgtcttg tcctcatccg atctagatta ttcgatattc ctcgacaata aacgattact
     1021 cagtgagggc aatcagagga acggataatg ataaataagc tgtaaaaaat agcaaaataa
     1081 caataatgat taaaagtatg taaaaaatag ggctttgaca aaaacgtgtc acagccctat
     1141 ttcgattaaa cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca
     1201 tgaggagctg ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa
     1261 ctcttgatta aacggcgttc aaaagcttgc atacgcggta ataaga
//
LOCUS       DANJLF010000195         1294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000195 DANJLF010000000
VERSION     DANJLF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..1244
                     /locus_tag="PWT25_002926"
     CDS             <1..1244
                     /locus_tag="PWT25_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL1022918.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DANJLF010000196         1287 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000196 DANJLF010000000
VERSION     DANJLF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..848
                     /locus_tag="PWT25_002927"
     CDS             <1..848
                     /locus_tag="PWT25_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1022919.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DANJLF010000197         1275 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000197 DANJLF010000000
VERSION     DANJLF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(61..279)
                     /locus_tag="PWT25_002928"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="PWT25_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="PWT25_002929"
     CDS             complement(545..739)
                     /locus_tag="PWT25_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022920.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="PWT25_002930"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="PWT25_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          450 a          214 c          174 g          437 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taatt
//
LOCUS       DANJLF010000198         1271 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000198 DANJLF010000000
VERSION     DANJLF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            265..957
                     /locus_tag="PWT25_002931"
     CDS             265..957
                     /locus_tag="PWT25_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL1022921.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          554 a          144 c          183 g          390 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa
     1021 tattatacaa tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac
     1081 tcgaaagagt agtcttttta ttattctcgc ttattattga tatgaaaatt tcgatttgac
     1141 aaaaggtacc gaataatttt gtaatatgaa ttattatcaa gaagtaatta tgatgataac
     1201 atatggaaac tagttagtaa taataacagg tgattaaact tttgaacgac ttgtaattct
     1261 atattttttc t
//
LOCUS       DANJLF010000199         1263 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000199 DANJLF010000000
VERSION     DANJLF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            18..1097
                     /locus_tag="PWT25_002932"
     CDS             18..1097
                     /locus_tag="PWT25_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1022922.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          375 a          301 c          310 g          277 t
ORIGIN      
        1 gacaaggatg aattcacatg acgcaaaccc atgataacac agtttcacgc aagggaaaac
       61 atctttccta ctcagaacgt tgccaaatcg ctatcctgaa aaaggaaggc tattccaacc
      121 gtcaggttgc caaagtgctg ggacgtgttc cgcaaaccat aaacaatgag attgatcgcg
      181 gaaccatcac ccagttaaaa cgccaaacgc aaaatggaaa agtgtatgac acctactatt
      241 ctgtttatga cgctgatgca ggacaggcct tctatgaaaa gcaacgactg aactgtggca
      301 gacgccccaa atgggcgaac acaaatgcct tcattgattg ggcagatgac aaaatgttaa
      361 atgaaaaatg gtcgccggac gtcgtggtcg gatttgccag aaaacacgag ctgttcgatt
      421 gtgccatcat tcctggcaca accgctcttt atcaatggat tgaccgcgga atcatgcgaa
      481 caaagaacat ggacttgctt gaaaaactgt cccgtaaaat caaagcggtc cgcccaggac
      541 accgtcccaa caagcgtgtt ctcgggacgt caattgaaga acgccctaaa gaagttgaaa
      601 cacgggaaac gttcggtcat tgggaaatcg acacggtagt gggaaacaag accaagtcgg
      661 atgccgtgct gctgacgctg gctgaacggc agacacgctt tgaagtcatc ctaaagttga
      721 acgggaagga caccgaatcg gtggaccaag cggttcaaca gctaagagaa cgggctggcg
      781 ctaacttcga tcaattattt aagaccgtta catccgacaa tggctcggag ttttctgggc
      841 tacacgatgc tctgcaagat gtagctgacg tctatttcag ccacccctat gcttcttggg
      901 agcgtgggac gagcgaaaac cagcacaaat taattcgccg tttcgtcccc aagggaagcc
      961 cgattagtga ggtgagcgag aaccagattt tgcggatcca gcgttggatg aacgattacc
     1021 cgcggaaaat tttgggttat gcgacaccac atgatacatt cgtccaggca ttcaagcaag
     1081 aacggttggt ggcttgaccc attattcctt gataacgatc ctcactcggg acatggcttc
     1141 atgtctggaa agctggcctg gggccagaat cgtctgaaga ctatcatacc tcagttcatt
     1201 atcaaggcct cactgattta gaataacgag gcagttacgg tatcaaacaa atgagttacc
     1261 tgt
//
LOCUS       DANJLF010000200         1247 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000200 DANJLF010000000
VERSION     DANJLF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..1126
                     /locus_tag="PWT25_002933"
     CDS             <1..1126
                     /locus_tag="PWT25_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1022923.1"
                     /translation="LFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIM
                     LDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEV
                     YLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQ
                     YSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSI
                     NRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARL
                     LSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSL
                     AIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          495 a          147 c          204 g          401 t
ORIGIN      
        1 gttatttcta gagacagaag ttgacaaaat tgatattcaa aataaaaaga ttagttgtgt
       61 aaaaaaagat ggtacaagat tctctcaaga atatgacaaa gttatattag ctacaggttc
      121 aaagcaaatt atgctagata taccggggaa taatttagct aatatttttt cgataaagaa
      181 ttttccaaat gcaaaagaga tttttaaagc tttggatgat agtactatta aaaaagtagg
      241 aattattgga gcaggatata ttggagttga aattgctgaa gccataagaa aaagagggaa
      301 agaagtatat cttttcgatg ttgcagatag agtgttgtcg acttattacg accgtagttt
      361 ttcagataaa gttgaagaaa tactatcaaa aaatggaata catctttgtc tatcagaaag
      421 tcctaaaaaa tatttaggaa gaaaaaagat tgaaaaaata gtcactacaa gtggtaccca
      481 atactctatg gatatggtcg tacaagctat aggatttcta tctaatagtc caattgggga
      541 aaaggaactc ttgagagatg ttagtggagc atatctaact aatgaatacc agcaaacaaa
      601 tataaaagat atatatgcta ttggggattg tgctacaact ttttttacgt ccattaatag
      661 aagtagtgtt gatttttcta tttctaatgc tctaagaaca gcttatattg caactcaaca
      721 tattaacaat caagatttta caccttctgg ttcccaattt acaaatggct tttctatatt
      781 taattacaat ttttacgcag caggtttgaa tttaaaaaca gcacgattat taagtgagtc
      841 tatagattat atagaaatag aagatttcat tagacctgct tttttgagaa aaaatagtcg
      901 tgtaaaatta agaattagct ttgaaaaaaa tacaaagaga atagtaggtg tacaactttg
      961 tagtcaagaa gatttatctg gactattatc aatgttttct cttgctatag aagaacaaat
     1021 tacattagac aaattgaaaa tgttagatta tttatttcat cctagtttta gccaacccta
     1081 taattttatt ttaaaggcag taacaggatt aaacattgaa acataatcgt tactaaatga
     1141 aatcaagaaa tatactgtat tactcaattc attgaaaata tttatcataa tttttaatag
     1201 aaaacactat taaagagaaa aaagagaggt aaatcaaatg aaaaaat
//
LOCUS       DANJLF010000201         1193 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000201 DANJLF010000000
VERSION     DANJLF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..>1193
                     /locus_tag="PWT25_002934"
     CDS             <1..>1193
                     /locus_tag="PWT25_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDL1022924.1"
                     /translation="KKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETIVR
                     FEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKVSL
                     SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFICAD
                     GETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVVID
                     RFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWK
                     SFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPET
                     LDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIR
                     IFLIN"
BASE COUNT          394 a          232 c          231 g          336 t
ORIGIN      
        1 agaaaaagac gttgatcgtc gatgctgttt tctcacctgc tccccatacc tgtagaaatt
       61 gtggttctac tgtggtagat ggaaacggga aagtaattgt agtgaaaaat gggaagaagg
      121 aaacgattgt ccgctttgaa caatacaatc acatgccttt ggttatgcgc ttaaaaaagc
      181 agcgctatac ctgtaaaaac tgtcgaaccc attggacggc tcaaagttat tttgttcaac
      241 ccagacattc aatcgcgaat catgtcagat ataaaattgc ttctttactg actgaaaaag
      301 tatctttatc ttttattgcg aaaaattgtc aggtatcttt gaccaccgtt attcgtacgt
      361 tgaaagagtt taaaagctac ttaccaaagc aatctaagag gattctcccc agagtattga
      421 tggttgatga atttcgttcg cacgcttcca tagaagataa aatgagcttt atttgcgcag
      481 atggcgaaac aggacaatta attgatgtct tgcctacgcg taaattgcct cgattaacaa
      541 gctatttctt aggctgcgct aatcctgaag aagtaaaatt tttagtcaca gacatgaacg
      601 ccgcctactt ccagcttacc aaacgtattc tgccaaatgc gaaagtagtg attgatcggt
      661 ttcatattat caaacacatg aatcaagcct ttaatgagtt gcgtatccgt gaaatgaatg
      721 aacttcgtaa agtaggacag aaaagtcagg cagaaaaact gaaaaagaac tggcgctttt
      781 tgctaaaaaa tcgtgcaaac atcaaccatt atgaatacaa aacatggaaa agttttcgag
      841 caccaaaata cccatttctt acggaagcga tgatgattga tcgattgctt ggcttttcta
      901 cgtccctgaa ggaggcgtat ccctattttc atgaattagt tgaagccttt cgagacaaag
      961 accctgactt atttttctcc ttattggcag aacttcccga aacgttagat gacggctttc
     1021 gggaaaagct ccaaaacctt ctgacctatg aagaaggcat caccaacgcg atgatctatc
     1081 cttacaccaa tggaaaaata gaagcgaaga atacccatat aaagacaatg aaacgagtat
     1141 cctacggatt taaatcattt gagaacatga gaattagaat ctttttgatc aat
//
LOCUS       DANJLF010000202         1193 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000202 DANJLF010000000
VERSION     DANJLF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..1076
                     /locus_tag="PWT25_002935"
     CDS             <1..1076
                     /locus_tag="PWT25_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL1022925.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DANJLF010000203         1190 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000203 DANJLF010000000
VERSION     DANJLF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..855)
                     /locus_tag="PWT25_002936"
     CDS             complement(<1..855)
                     /locus_tag="PWT25_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022926.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGT"
BASE COUNT          391 a          214 c          120 g          465 t
ORIGIN      
        1 tgttcctata aaatcaagtc ctctacattt taaaggttca cttaaactat taatatagtt
       61 aataatgttg tctatccttt tctctttatc ttcaccttca atctggggag catatagtcc
      121 aatttcctcc caataatatg aatctctttc aattcccatg atggtagctt tggctaattt
      181 caaatcaaat aacgatttta gaattcttag tgtctcaata aagtcttctc tcttcaactc
      241 tttaatccat tgtgaatttt ctggattatc taaaatttca agttcatctt cattataagt
      301 atatttcaaa tttgacttgc ttaactcata gataaatgaa gtttcttgac tcataattct
      361 tttaagtact tgactataag tttcgtttga ctttgcccat ttcgctaatt ctaccactgt
      421 attcactaat aaatcgaaat aatgatgttc tcgttcaata gtctcagcat tttgaaaatg
      481 aaaatcacct ttataaactc cattcttttc cactgtttta ttaatatttt ttaaaaacca
      541 gaatgagttt gcaaatttag tcgtactttt tcgattaact aagttaataa ctcttccatg
      601 taataataag acaaaaatac ataaattttt agcttcctta ttattagata ctaattgatc
      661 aatcataggg agccattttt tgattttcac taattgattg ctcataaatc tttcactgga
      721 cttttgttca atcttaaatt gcaaaacttc taactcataa ttcccatctt ctgatttatt
      781 tttgggaaca actatcaaac ttttataaag tttatccaca tcaaaattta tcttttttgc
      841 ataatttgta gccattaata catctcctta ttcataataa atttcatatg acgctcctct
      901 caaaatttac cggtagacta gaaaaaagaa taaaagatac gtcaattcac actttattct
      961 taaaaaaaaa ctggtctctc taccggtaat atcgattgag tccgccttta tcgactttat
     1021 ccttttttta tattaaattt tttcaccggt agacagggag agaaagaata aactaaaaag
     1081 cccttaattc ttaatgtttt cacctcctta ccgttcctac cgctaaaatt taatataact
     1141 aaattctata acattagaag aaattttaaa tacctttgat aaaaaatatt
//
LOCUS       DANJLF010000204         1160 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000204 DANJLF010000000
VERSION     DANJLF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            14..1141
                     /locus_tag="PWT25_002937"
     CDS             14..1141
                     /locus_tag="PWT25_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1022927.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          419 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa
//
LOCUS       DANJLF010000205         1144 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000205 DANJLF010000000
VERSION     DANJLF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="PWT25_002938"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="PWT25_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL1022928.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          380 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc ccattttcat
     1141 ttta
//
LOCUS       DANJLF010000206         1098 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000206 DANJLF010000000
VERSION     DANJLF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            14..424
                     /gene="spx"
                     /locus_tag="PWT25_002939"
     CDS             14..424
                     /gene="spx"
                     /locus_tag="PWT25_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL1022929.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            438..794
                     /locus_tag="PWT25_002940"
     CDS             438..794
                     /locus_tag="PWT25_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL1022930.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          384 a          163 c          211 g          340 t
ORIGIN      
        1 aatgagtgag aaaatgataa aagtatatac ttcgcctagc tgtacttcct gtagaaaagc
       61 aagagcatgg ttgcaagcga atcaacttga atttgaagag aagaatattt ttgccgaacc
      121 gttaacagaa agtgagatca aaaagatctt ggcagcgact gaaggaggag ttcaagatat
      181 tatttccact cggtcgaaaa tttacgaaaa gctgggcata gattttaacg aattaacttt
      241 aaagcaaatg atcgtcttat tcaaagaata tccttcatta ttgagacgac ctattttgat
      301 tgatgaaaaa agaatactaa tcggatttaa tgaagatgag atccggtcat ttgtccctcg
      361 tgaaattcga tgtgttgcca gaaatctgat ctatttacaa aacaataaca tcttaaattc
      421 atagggaggg agccttcgtg gaagtgaaag tctatggatc agcgcactgt gtaacaagtc
      481 tgaaggcgaa acaatggtta gaacaccatc aattgcctta tcaatttgtt gacttagaag
      541 aacgcgatct aagtacagaa gaagcagaag aattatgcag ttttgaagaa gtgcaagcag
      601 aacagctatt tgcgacttgg tctgaggctt ttcacaacat tgcgattgac ctttcctgtg
      661 taggaaaaaa acaactgatt tttttatgca cacaacatac caatctcctc agacgtcctt
      721 tgattattat cgatgaagtt ctatttattg gatacaatga gcagttgatg gaacagctta
      781 tcttaaataa atagacttta actgttttaa tcaagtcact gagcatttca tacagttgta
      841 acagtcgaac acttagcatc aagcgtgtta tgtgttcttt ttttgacttt atttttctac
      901 tatagagaca tgctcaattt atgtggtcaa aattcaaatc taggtgggga tatagagtta
      961 aaaatatatt gacatgtgta aatttacatg atatattcat ttctgttgtt ttaagtagca
     1021 aagcaataaa atagtatttt aaaaaacata taaaatagtt gttgacatgt tagaaatgac
     1081 atgataagat aactgagt
//
LOCUS       DANJLF010000207         1078 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000207 DANJLF010000000
VERSION     DANJLF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            249..>1078
                     /locus_tag="PWT25_002941"
     CDS             249..>1078
                     /locus_tag="PWT25_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1022931.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          378 a          152 c          183 g          365 t
ORIGIN      
        1 taagttattc ttttattact atgtttgggt tttatagcaa agaaagttta ataagagttt
       61 ggacctagca aacaatatta atgaggtcat taaatcttat tatctttagt gatataacga
      121 aatatctaca cagacaaaat gatagcgtaa aatattttgt gtaaatagat atttagggta
      181 gaaaaaaaga aagtccattc tgtaaaatta aagttaccac aaccgaaatt ttataggagg
      241 actttctcat gcccaatttt actacagaaa ttatggaaac actaatcaat aaaggtgatt
      301 tagatgaatt atttcgatgt catttagaat tagctgttaa ttcattattg caggctgaat
      361 taacagcttt tcttgattat gaaaaatatg atcgtgctgg gttcaattca ggtaattccc
      421 gtaatgggaa ttattcacgt tcattcagaa cagagtatgg agaattaaat ttggtaattc
      481 ctagagatag aaatggtaag ttttctcaac aaactttacc tgcttataaa agaaccaatg
      541 actctttaga aaccactatt attcagctat ttcaaaaagg aattactatg tctgaaatct
      601 ctgaattgat tgaaaaaatg tacgggcatc actatacgcc acaaaccatt tcaaatatga
      661 ctaaaatagt ttctgaagat attattgcct ttaaagaaag gtccttagaa tccagatact
      721 ctgtcatttt tatggatgct actcacattc ctttaaaaag acaaactgtt tcaaaagaag
      781 cagtttatat tgtaattggc attcgattag atggaaccaa agaggttctt gggtttagtc
      841 ttgctccaac tgaatctgct tatgtttgga aagagattct tcaagaccta aaagaccgtg
      901 gtttaaaaga ggttttatta gtcgtaactg atggtttaag tggcattaac gatagtattc
      961 atagtgttta tccaaatgct caatttcaac aatgttgtgt ccatatatct agaaatatcg
     1021 ctcataaggt tcgtgttagt gatcgacaag aaatctgtag cgatttcaaa ttagttta
//
LOCUS       DANJLF010000208         1063 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000208 DANJLF010000000
VERSION     DANJLF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..704
                     /locus_tag="PWT25_002942"
     CDS             <1..704
                     /locus_tag="PWT25_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1022932.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          360 a          172 c          177 g          354 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataagg gttctgttgc aaagttttaa ataaagaata
     1021 aaatccctta cggtatctat gatttaagct gggattccca ata
//
LOCUS       DANJLF010000209         1062 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000209 DANJLF010000000
VERSION     DANJLF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            4..963
                     /locus_tag="PWT25_002943"
     CDS             4..963
                     /locus_tag="PWT25_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL1022933.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          369 a          200 c          210 g          283 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DANJLF010000210         1060 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000210 DANJLF010000000
VERSION     DANJLF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(38..994)
                     /locus_tag="PWT25_002944"
     CDS             complement(38..994)
                     /locus_tag="PWT25_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022934.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DANJLF010000211         1051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000211 DANJLF010000000
VERSION     DANJLF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="PWT25_002945"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="PWT25_002945"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL1022935.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DANJLF010000212          995 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000212 DANJLF010000000
VERSION     DANJLF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(26..913)
                     /locus_tag="PWT25_002946"
     CDS             complement(26..913)
                     /locus_tag="PWT25_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL1022936.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DANJLF010000213          994 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000213 DANJLF010000000
VERSION     DANJLF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..>994)
                     /locus_tag="PWT25_002947"
     rRNA            complement(<1..>994)
                     /locus_tag="PWT25_002947"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DANJLF010000214          959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000214 DANJLF010000000
VERSION     DANJLF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(107..511)
                     /locus_tag="PWT25_002948"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="PWT25_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="PWT25_002949"
     CDS             complement(570..>959)
                     /locus_tag="PWT25_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1022937.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DANJLF010000215          931 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000215 DANJLF010000000
VERSION     DANJLF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..>931
                     /locus_tag="PWT25_002950"
     CDS             <1..>931
                     /locus_tag="PWT25_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL1022938.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DANJLF010000216          902 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000216 DANJLF010000000
VERSION     DANJLF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            263..886
                     /locus_tag="PWT25_002951"
     CDS             263..886
                     /locus_tag="PWT25_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1022939.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          332 a          129 c          167 g          274 t
ORIGIN      
        1 gttttaaaat aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac
       61 ttaaaggagg tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt
      121 tgttgtttct tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt
      181 tttcagcggt ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata
      241 gattggatag gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca
      301 aagtttagct cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga
      361 agacgattta aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga
      421 gctgcaagag cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga
      481 tcgattagca agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg
      541 tgcttctatt gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag
      601 agacttgcta acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa
      661 aaaaatcaaa gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac
      721 tggtaaaaag attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga
      781 aattgttgat ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt
      841 tacaagaaaa accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag
      901 aa
//
LOCUS       DANJLF010000217          856 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000217 DANJLF010000000
VERSION     DANJLF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            83..>856
                     /locus_tag="PWT25_002952"
     CDS             83..>856
                     /locus_tag="PWT25_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1022940.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANA
                     NKHNFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
BASE COUNT          285 a          158 c          165 g          248 t
ORIGIN      
        1 ataaaatgcc agactgtaga caaagtccat tttatggatg atgtctacag tcttttcttt
       61 tataatagat ttatgaaaac gaatgcaagg agtgatagct atgatgtcta aaaacaatac
      121 gaatggtaga aatcaatttg cgatgcttac gattgatgat ctagttcctc aagatcatct
      181 cgttcgaaag attgatgccg cgcttgattt tgaatttatt tatccaatcg tcgaagccac
      241 ttactccgat ttaggtcgcc caagtatcga cccagtgatt ctgatcaaat tggtgtttat
      301 tcagtattta ttcggtatcc gttccatgcg acaaaccatt aaggaagtcg ataccaatgt
      361 cgcttaccgc tggtttttag gctactcctt cgaagagaaa atcccccatt tctcaacttt
      421 tggaaagaat tatgttcgcc gatttagaga aacgacggtg tttgaggata tttttgccta
      481 tattttagaa caagcagtta aagcaggttt tgtaacagag gataattttc atctagattc
      541 tacccacatc aaagctaacg ccaataaaca taattttacg aaagaaatga cccatgatga
      601 agcgaaagcc tatcaggatg aattagaaga tgagatcaat cgtcaacgaa tagaggctgg
      661 aaaacgccct tttactttgg acttagaaaa ggaagtgaag ctgaaagaaa gaaaaataag
      721 taaagctgat ccggaaagcg gctactacgt caaaggcgaa caagaaaaac aatttgctta
      781 ttctgcccat acttcatgcg acgacaatgg atttattctt agcacaatca tcactcctgg
      841 aaatattcat gacagt
//
LOCUS       DANJLF010000218          826 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000218 DANJLF010000000
VERSION     DANJLF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            136..309
                     /locus_tag="PWT25_002953"
     CDS             136..309
                     /locus_tag="PWT25_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022941.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          277 a          135 c          104 g          310 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actcca
//
LOCUS       DANJLF010000219          825 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000219 DANJLF010000000
VERSION     DANJLF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            20..>825
                     /locus_tag="PWT25_002954"
     CDS             20..>825
                     /locus_tag="PWT25_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HDL1022942.1"
                     /translation="MEILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGF
                     NSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQK
                     GITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIP
                     LKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVV
                     TDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV"
BASE COUNT          282 a          120 c          151 g          272 t
ORIGIN      
        1 cgattttact acagaaatta tggaaatact aatcaataaa ggtgatttgg atgatttgtt
       61 tcgtcgtcac ctagaattgg ctattaacgc tttattacaa gctgaattaa cggtatttct
      121 tgactatgaa aagtatgata gagctggatt taattcaggt aattcccgta atgggaatta
      181 ttcgcgctct tttaaaacag agtacggtga attgaattta gtaataccta gagataggaa
      241 tggagacttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
      301 cactattatt cagctagttc aaaaaggaat tactatgtcc gaaatctctg atctaatcga
      361 aaaaatgtat ggtcattatt acaaaccaca aactatttca aatatgagta aaattgtgtc
      421 tgaagatgtt tcggcattta aagaaagaac tttagaagct aaatactcag tcatttttat
      481 ggatgctact catattccct taaaaagaca aaccgtatca aaagaagctg tctatatagt
      541 aattggtatc aggttggatg gaactaaaga ggttctagga tttactatag ctccaactga
      601 atctgcttat gtttggaaag agattcttca agatctaaaa gaccgtggtt tagaagaggt
      661 tttgttagtc gtaacagatg gtttaagtgg tattgacgat agtattcata gtgtttatcc
      721 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
      781 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gttta
//
LOCUS       DANJLF010000220          811 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000220 DANJLF010000000
VERSION     DANJLF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..672
                     /locus_tag="PWT25_002955"
     CDS             <1..672
                     /locus_tag="PWT25_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1022943.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DANJLF010000221          777 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000221 DANJLF010000000
VERSION     DANJLF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(31..>777)
                     /gene="aph(2'')-I"
                     /locus_tag="PWT25_002956"
     CDS             complement(31..>777)
                     /gene="aph(2'')-I"
                     /locus_tag="PWT25_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDL1022944.1"
                     /translation="NKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYK
                     EIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYI
                     LLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGI
                     IDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYP
                     IETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          259 a          126 c           52 g          340 t
ORIGIN      
        1 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
       61 ttttctacca ttttcgataa attcctgttt aatattttta attccataaa caatagtttc
      121 aataggataa tattcttcaa ctatatcttg atattctttt gctttctcaa tatctatatt
      181 tccatacatt cttaatatat cttctccaaa atttgttcct atttcttctt cactatcttc
      241 aagtaagtat ataaaatcac aatattcatc tataattcca gaatctccaa aatcaattat
      301 tccagttaat ctattattgc catctaacaa tagatgatta caactaaaat cattatggca
      361 taaacacttt ttaccctcaa aaactgttgt tgcatttagt ctttccataa aactttctat
      421 ataatctttt tctatatcag ttaaatcatt ataaatagtt tcacgcaaca atatatactc
      481 ttctaataca ttttgtttat tatcaatagt acattcacta atatctgtat aatctaaacc
      541 gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac aaattttgtt cttcttctga
      601 catagtagaa taaatttctg gtgttaaaaa agttccttta atttctttat aacctagtat
      661 agataattca tcactaatat acgaatattc aatattagga atttttacat tagtttctaa
      721 atttgtattt aaaaaattat atattgcttt ttcttttgca taaccttttt tcttatt
//
LOCUS       DANJLF010000222          765 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000222 DANJLF010000000
VERSION     DANJLF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..276)
                     /locus_tag="PWT25_002957"
     CDS             complement(<1..276)
                     /locus_tag="PWT25_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022945.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          259 a          129 c          128 g          249 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa acactcccga tgaagtagac ctttgaaaac tgaacaaagt
      721 aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa gacaa
//
LOCUS       DANJLF010000223          754 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000223 DANJLF010000000
VERSION     DANJLF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(91..>754)
                     /locus_tag="PWT25_002958"
     CDS             complement(91..>754)
                     /locus_tag="PWT25_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HDL1022946.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          204 a          177 c          145 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gcgc
//
LOCUS       DANJLF010000224          733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000224 DANJLF010000000
VERSION     DANJLF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..>733
                     /locus_tag="PWT25_002959"
     rRNA            <1..>733
                     /locus_tag="PWT25_002959"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DANJLF010000225          733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000225 DANJLF010000000
VERSION     DANJLF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            309..707
                     /gene="spxA"
                     /locus_tag="PWT25_002960"
     CDS             309..707
                     /gene="spxA"
                     /locus_tag="PWT25_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL1022947.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          264 a          104 c          134 g          231 t
ORIGIN      
        1 cataaaaaat cttaagaaat cggtttcatt tagcttttga gataaaataa tagtacagtt
       61 ttatacgctg tttcatatgt ttagaaaatt tgaattagat ttgaaaactc taatttttaa
      121 atgataaaaa aagcgagaat gctggtttta ctgtatatta accttagata actttcattt
      181 ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt tgattatgta taatagaaac
      241 agatgggaga ttattatgat cttctgttac aaaaaagtga ttcttatcac gaaaaggagt
      301 ggtgcacgtt gttaacatta tatacttccc caagttgtac ttcctgcaga aaggctcgtg
      361 cgtggttgca agaacatcag attccatttg ttgaacgtaa tattttttca gaaccattga
      421 acagttctga attaaaagcg attttacaaa tgacagaaga cggaacagaa gaaatcatct
      481 cgacacgttc caaagtattc caaaaactga acatggattt agatgatctg ccgttacaag
      541 aattattgga acttgtgcaa aacaatcctg gattgcttcg ccgtccaatc atgattgatg
      601 ataaacgtct gcaagttggt tttaatgaag atgaaattcg tcggttccta ccgcgcgatg
      661 tacgacaatt agaattacgt caagcacaat tgatggctgg attatagaag gataatacga
      721 aacttggaaa aga
//
LOCUS       DANJLF010000226          733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000226 DANJLF010000000
VERSION     DANJLF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(59..418)
                     /locus_tag="PWT25_002961"
                     /pseudo
     CDS             complement(59..418)
                     /locus_tag="PWT25_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          209 a          133 c          149 g          242 t
ORIGIN      
        1 ttagactttt ttggagtgga gaaatccact ctttttttca tgctctaaaa tgatattttt
       61 acacaaccgt aacggttgtt ttacgaaatt ggacgggact ttgccagttt aatttctgtt
      121 ttatccgttc attattgtag taatagatat agctatcgat ggctgatttt aattcctcga
      181 aacagttata aactttcccg tgaaaaatct cttgttttaa taagccaaaa aaattctcca
      241 taagtgaatt atctaagcag ttccctttcc gagacatgct ttgaaagatt ttgtactcct
      301 tcaatttgtt tctataagct ttcatttgat acgaccaacc ttgatctgag tgaaacgtac
      361 gacgataggg acaatcgtta gtggtctgga tagcttcctc caaaccttcc attaccgcac
      421 gtgtttctga atggtggtag cgaccgattt gaacggctag ggcggaaata cggtgcaaat
      481 gcacggtggt ttcatgcaat gcttacaggg cgtattttgt aagggtatgt aacggagata
      541 atcccgacgg taatggatgt gagaggccaa gccgacgctg acggtagggc aaacaaacat
      601 agttgcactc tagcgatcgt tatttatttt taacggtcaa actagggtac aactatgctt
      661 aaaacacttc gcctcaactt ctggtagcaa aagtgatttt gaaagtcaaa atatttttta
      721 tgagttattt ata
//
LOCUS       DANJLF010000227          716 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000227 DANJLF010000000
VERSION     DANJLF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
BASE COUNT          301 a          108 c          126 g          181 t
ORIGIN      
        1 ttatagagcc ggaattcctt aggcagcttt tgctgtgtag aaaggaataa ataataacga
       61 aagttcaaaa gctaatgccg actattctag acattaaaga agacgaaaac gaacgataaa
      121 gaaagaaaag ttatgaataa tcgtttattg aaaaaaactt gaaaaaatac aaaaaattcg
      181 ttgaccaagc taaatttagt tggtatatta ttacttgtcc aaaagacaga agaaaatatt
      241 tattcaaaaa cataacaaaa aagttattga catgttacta acgacatgat aagataactg
      301 agtcgttaaa aaagaaccac ttgcgaagct tgattctatc gaaaaaaact tcaaaaaagt
      361 tcttgacaaa taacaaacga taagttatga taaatgagtt gccgaaagat ttcgaaaaac
      421 gaaatcgaaa aaaactttta aaaaagagtt gacaatcgct tgtctctctg atatgatata
      481 aaagttgcta cggcagccaa aaatgaatcg ggaaacactc tcgatgaagt agacctttga
      541 aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc ttgattcaat tcaagacaac
      601 aaacattttt aacaagcaag caatatgcta gcaaacaaat tgagcttaac aatcgcaaga
      661 ttgttcgaac tttttatgag agtttgatcc tggctcagga cgaacgctgg cggcgt
//
LOCUS       DANJLF010000228          712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000228 DANJLF010000000
VERSION     DANJLF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..446)
                     /locus_tag="PWT25_002962"
     CDS             complement(<1..446)
                     /locus_tag="PWT25_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022948.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DANJLF010000229          696 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000229 DANJLF010000000
VERSION     DANJLF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..437
                     /locus_tag="PWT25_002963"
     CDS             <1..437
                     /locus_tag="PWT25_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022949.1"
                     /translation="NFITDHPESMYFHEDIYHQGNSKELEVVLRYNLFSFTSEFYFLR
                     HKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINEVSILEVKKLVI
                     HLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            501..>696
                     /locus_tag="PWT25_002964"
     CDS             501..>696
                     /locus_tag="PWT25_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL1022950.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMG"
BASE COUNT          263 a           81 c          105 g          247 t
ORIGIN      
        1 ataattttat cacagatcat cctgaaagta tgtattttca tgaagatatt tatcatcaag
       61 gaaatagtaa agaattagaa gttgtcctta gatataattt attttcgttt acgagtgaat
      121 tttacttttt aagacataaa gagagaattg taggaatggt ttctataatt gttaatccat
      181 ttcaaaactt atcctacagt atagtaaatt acctcatatg tgagcctgca tatactacga
      241 ctattttgac aacagtatta gaaaatatta acgaagtttc aattttagaa gtaaagaagc
      301 tagtgattca tttgctaaat tcaacagaag atgaatcact aatcccctat ttaaaagatt
      361 cacatttttt taaagaagcg gtactatcta aagaatatag agagcatgat gttttgagat
      421 acgcttacat ttattaaata aaatttctga tagtatttta ataattcgga tcatatcata
      481 agatatagga gggaaaagaa gtgattttat tgattttgtc tctcgtatta ctagttgtat
      541 ctaatctaat gaaaaataat cctcctacaa aaattaattc tttttatggc tttagaacta
      601 aaaagtcaat gaaaaatgaa aaaagttggc atttagcaca aaaattgttg ggggattttt
      661 caaaaaaaaa agttatccca gtatttttga tgggta
//
LOCUS       DANJLF010000230          635 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000230 DANJLF010000000
VERSION     DANJLF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..270)
                     /locus_tag="PWT25_002965"
     CDS             complement(<1..270)
                     /locus_tag="PWT25_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022951.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="PWT25_002966"
     CDS             complement(276..>635)
                     /locus_tag="PWT25_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL1022952.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DANJLF010000231          580 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000231 DANJLF010000000
VERSION     DANJLF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
BASE COUNT          189 a          115 c           83 g          193 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa
//
LOCUS       DANJLF010000232          530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000232 DANJLF010000000
VERSION     DANJLF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..>530)
                     /locus_tag="PWT25_002967"
     CDS             complement(<1..>530)
                     /locus_tag="PWT25_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL1022953.1"
                     /translation="EFYYKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPP
                     KIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFN
                     SSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEK
                     LFVRKVKSDYSDKLLN"
BASE COUNT          172 a           74 c           69 g          215 t
ORIGIN      
        1 ctatttaata gtttatcaga gtaatccgat ttaacttttc ttacaaataa tttttcactt
       61 tcttttattt gatctatatc cctttcatca aaccatttgt tcatggaagg atcaataata
      121 tgaatatttg ctaatttcga agtttgtaaa gtagtttctt caatattttt caattcaagt
      181 ggtaaatctg gtcctccaat aacattttta tttttgaacg aggaattaaa aaaaagtgta
      241 tgaaaaaatt tttcatctgg ggcaaacata cctattttaa aattcaggta gtcttgttga
      301 ttttgttcat acatttgtat taattctgtt aggcattctt tcgacaaagc ccaccattga
      361 gaaccatgat acaaatcaaa tttatggtgt ctaatttttg ggggaagttt aatcatcctc
      421 agacaacgat taattatagc tctaaaaatt ttaaatgcag ttgtgttatt cctattaatt
      481 ctaggataat catgtttttg atatacatct atatgtttat aataaaattc
//
LOCUS       DANJLF010000233          528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000233 DANJLF010000000
VERSION     DANJLF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            141..398
                     /locus_tag="PWT25_002968"
     CDS             141..398
                     /locus_tag="PWT25_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022954.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          223 a           66 c           74 g          165 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaa
//
LOCUS       DANJLF010000234          526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000234 DANJLF010000000
VERSION     DANJLF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="PWT25_002969"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="PWT25_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL1022955.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="PWT25_002970"
     CDS             complement(349..>526)
                     /locus_tag="PWT25_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022956.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DANJLF010000235          514 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000235 DANJLF010000000
VERSION     DANJLF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..132)
                     /locus_tag="PWT25_002971"
     CDS             complement(<1..132)
                     /locus_tag="PWT25_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL1022957.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DANJLF010000236          510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000236 DANJLF010000000
VERSION     DANJLF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            1..510
                     /locus_tag="PWT25_002972"
                     /pseudo
     CDS             1..510
                     /locus_tag="PWT25_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DANJLF010000237          507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000237 DANJLF010000000
VERSION     DANJLF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..506
                     /locus_tag="PWT25_002973"
     CDS             <1..506
                     /locus_tag="PWT25_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL1022958.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIK
                     VLMGITA"
BASE COUNT          176 a           96 c          103 g          132 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct actgaaatca
      481 aggtattaat gggaataaca gcctaag
//
LOCUS       DANJLF010000238          500 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000238 DANJLF010000000
VERSION     DANJLF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.251"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..>500
                     /locus_tag="PWT25_002974"
     CDS             <1..>500
                     /locus_tag="PWT25_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1022959.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGS"
BASE COUNT          206 a           46 c           90 g          158 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt
//
LOCUS       DANJLF010000239          485 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000239 DANJLF010000000
VERSION     DANJLF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(62..259)
                     /locus_tag="PWT25_002975"
     CDS             complement(62..259)
                     /locus_tag="PWT25_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1022960.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DANJLF010000240          479 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000240 DANJLF010000000
VERSION     DANJLF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.253"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            24..>479
                     /locus_tag="PWT25_002976"
     CDS             24..>479
                     /locus_tag="PWT25_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL1022961.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT          168 a           69 c          109 g          133 t
ORIGIN      
        1 tataaattgt tggggttgag taaatggtca tgaatgctat ttcggcgatg aaaaaaacaa
       61 aacagcagct acatgaagcg gggtttaagc tgacaccgca aagagaagct actctgcttg
      121 tgcttttgga aaatgagaaa gagcatttat ctgcagaaga aatcttcttt ttagtgaaaa
      181 aaaagagtcc ggaaattgga cttgctactg tttatcgaac ccttgaaatc ttgacagaat
      241 tgaaaattgt cgataaagta agttttaatg atggattagc ccgttatgat ttgagaaaag
      301 aaggcgccaa acatttccat catcatttac tatgcttgaa ctgcggcacg atcgaggaag
      361 tagaagagga tcttttactt gaagtggagc aagtagtaga agaacgttat cattttttag
      421 taaaagatca tcggctaacg ttccatggaa tctgtcagga atgctatgaa aaagaaaaa
//
LOCUS       DANJLF010000241          462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000241 DANJLF010000000
VERSION     DANJLF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..430)
                     /locus_tag="PWT25_002977"
     CDS             complement(<1..430)
                     /locus_tag="PWT25_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL1022962.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DANJLF010000242          461 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000242 DANJLF010000000
VERSION     DANJLF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.255"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            98..292
                     /locus_tag="PWT25_002978"
     CDS             98..292
                     /locus_tag="PWT25_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022963.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          194 a           59 c           80 g          128 t
ORIGIN      
        1 gattcttttt atttatcata aagatataga tacataatca ttttcactta catatatttg
       61 aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag aagtaaaaac
      121 aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc ctttacttgg
      181 ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa agaacgagct
      241 agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt aaatccaatg
      301 tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt atagaattag
      361 aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac catatgatta
      421 tttcaatatg tatcctggaa ttggagatct cgtagagcta t
//
LOCUS       DANJLF010000243          458 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000243 DANJLF010000000
VERSION     DANJLF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            7..438
                     /locus_tag="PWT25_002979"
     CDS             7..438
                     /locus_tag="PWT25_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDL1022964.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          191 a           56 c           62 g          149 t
ORIGIN      
        1 aataaaatga ttggagagaa aatgatggat caaaaagcgt ttatcgaatc tattgattct
       61 acaatcgata gattaaaaaa agaaattcga ttttataata gagtaattgg tatcggaaat
      121 attataaaaa tagtcttatc agctagtatc ccaatattaa ttgatcaggc ttccgaacac
      181 aggtctttac tattgattgt ttcaattgct tcagcaataa ttacaattat acaaagtggt
      241 atgtctgcat ttaattatca agataaagta cagacttcga cagagttatt aatgaaaatt
      301 gaaaaagaaa agttgttata tataacgaaa acatccccat ataataaaac agatgaagaa
      361 aatttccatt taatcgttac aacccttcaa actgaattaa atgatattat ctctgatttt
      421 aatcaagtta ataactaaaa atttatttaa ttagaaag
//
LOCUS       DANJLF010000244          454 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000244 DANJLF010000000
VERSION     DANJLF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
BASE COUNT          147 a           88 c           52 g          167 t
ORIGIN      
        1 acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta
       61 gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt
      121 ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac
      181 tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact
      241 ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg
      301 tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca
      361 acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt
      421 atacactact attcagccca ttcaacgacc aatg
//
LOCUS       DANJLF010000245          438 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000245 DANJLF010000000
VERSION     DANJLF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.259"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
BASE COUNT          128 a           75 c           59 g          176 t
ORIGIN      
        1 tgaagaaatt gtgcattatt tcttcacatt tattaggtta tagtttaaga aagtttaaac
       61 attgcgcaat ttaattattt tagctgtttc ttttcataaa aaaacatgtt gtgactctga
      121 gacaaatcat agtccatagt gatgtctcta gagtaactac gcctaatatt acgaggcatc
      181 atattaatct atcagcacta gtacctaaac tagcatccct ttcgaatatt ttttcttata
      241 taataaagtt tgctttcact cctctaatct caccaaatcg actggcttgc gttgtgtgat
      301 actaggaact gattggttta gaacgacttt tctattgtaa tcatattttt cgccatcctc
      361 gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg tattaaataa tttaattttt
      421 ttgttcctat ttatttaa
//
LOCUS       DANJLF010000246          415 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000246 DANJLF010000000
VERSION     DANJLF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..339)
                     /locus_tag="PWT25_002980"
     CDS             complement(<1..339)
                     /locus_tag="PWT25_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022965.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DANJLF010000247          409 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000247 DANJLF010000000
VERSION     DANJLF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..379
                     /locus_tag="PWT25_002981"
     CDS             <1..379
                     /locus_tag="PWT25_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDL1022966.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DANJLF010000248          404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000248 DANJLF010000000
VERSION     DANJLF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..>404
                     /locus_tag="PWT25_002982"
     CDS             <1..>404
                     /locus_tag="PWT25_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022967.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DANJLF010000249          373 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000249 DANJLF010000000
VERSION     DANJLF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            12..>373
                     /locus_tag="PWT25_002983"
     CDS             12..>373
                     /locus_tag="PWT25_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022968.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNP"
BASE COUNT          157 a           41 c           64 g          111 t
ORIGIN      
        1 tgtaaaagga catgatgatt atggaagaaa tagaatggaa taatctgact caaaataata
       61 aaaataattc tggatgtgga attatcttta tgtttgttat agttttggga atagtagtac
      121 ttttaataac tgtcaagata gtagattact ttgcgaatac tcctgaagaa caaatacaga
      181 gaaaacttcc tgagtttgaa agagtaccac cttatgatcc aagagataat tcatttcaag
      241 taaaaaaatt aaaagagtct ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg
      301 acttcaaaga aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat
      361 ttatttttaa ccc
//
LOCUS       DANJLF010000250          366 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000250 DANJLF010000000
VERSION     DANJLF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            79..282
                     /locus_tag="PWT25_002984"
     CDS             79..282
                     /locus_tag="PWT25_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HDL1022969.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          147 a           40 c           77 g          102 t
ORIGIN      
        1 tgatatttaa tattacaagc aatcttaaga aattcgaaaa ggaaaaaatg attatgaaaa
       61 agaaagaaaa agaaagtatt gaaacattgt gttattctag gaatattagg aacttgtcta
      121 gctggcattg gtacaggaat agacgttgac gcagctactt actatggaaa tggtctttat
      181 tgtaacaaag aaaaatgttg ggtaaattgg ggacaatcat ggtcagaagg gttaaaacgt
      241 tggggagata atctttttgg tagttttagt ggaggacgat aatgacttca ataaacattg
      301 atcacacaaa aaaagtcata catgaattat acaatagttt aatgacacga cctaatcaga
      361 cgaaag
//
LOCUS       DANJLF010000251          360 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000251 DANJLF010000000
VERSION     DANJLF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.268"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
BASE COUNT          128 a           58 c           61 g          113 t
ORIGIN      
        1 tgaaaaaaag tgagaaaaaa ggcttacaat tggagtggta gttaattagt gacttatttt
       61 tgattttata gcactgatac tataaaatat agatatcatc atattacaca atcttaatac
      121 taacttaaaa aatatctcct ttcacaagta tggtgataaa attcgttccg ggctaccttt
      181 ttaggtagcc tactttaatc tttatacctt tctggatcaa cgaaagtata ctttatatag
      241 tcataacgcc gatgatcgct acgtgcgtcc ggcacgtcag tcatgaatcg gctacactag
      301 actagacaga aaaaataagg tgtgtagaat aataaaaaac acactggagg atttttcatg
//
LOCUS       DANJLF010000252          344 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000252 DANJLF010000000
VERSION     DANJLF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.269"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
BASE COUNT          123 a           51 c           38 g          132 t
ORIGIN      
        1 aaaaaagaaa ccacgtctac aagcgtggtt tcttttttgt gtctatgtaa aaaacagcta
       61 caaataaaaa tcatgtacca agctgtatta catgattttt tataagacta tccagcatat
      121 acatgtgtga tctaacattt agcttaaaaa ttagaaaatc catttttctt ttatcattac
      181 atatacttta tccatttact tgtttataat taaatcggct aaatacacac tagtctttta
      241 tttagtgcca cttcttaata atcccattgt tattatcaaa taaaaaatac aatatatggt
      301 tttgaataat tttttgaaag aaggcttcat tttgagttat ctga
//
LOCUS       DANJLF010000253          328 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000253 DANJLF010000000
VERSION     DANJLF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.270"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            52..>328
                     /locus_tag="PWT25_002985"
     CDS             52..>328
                     /locus_tag="PWT25_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL1022970.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          104 a           60 c           63 g          101 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagt
//
LOCUS       DANJLF010000254          321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000254 DANJLF010000000
VERSION     DANJLF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(69..321)
                     /locus_tag="PWT25_002986"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="PWT25_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DANJLF010000255          309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000255 DANJLF010000000
VERSION     DANJLF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.276"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="PWT25_002987"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="PWT25_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL1022971.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           97 a           45 c           50 g          117 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatat
//
LOCUS       DANJLF010000256          308 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000256 DANJLF010000000
VERSION     DANJLF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.277"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(76..>308)
                     /locus_tag="PWT25_002988"
     CDS             complement(76..>308)
                     /locus_tag="PWT25_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1022972.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DANJLF010000257          282 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000257 DANJLF010000000
VERSION     DANJLF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.281"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..>282
                     /locus_tag="PWT25_002989"
     CDS             <1..>282
                     /locus_tag="PWT25_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDL1022973.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DANJLF010000258          281 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000258 DANJLF010000000
VERSION     DANJLF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.282"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            1..281
                     /locus_tag="PWT25_002990"
                     /pseudo
     CDS             1..281
                     /locus_tag="PWT25_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          111 a           56 c           54 g           60 t
ORIGIN      
        1 aaacaacaaa tcgtcgatct ctatctcgct ggtaagcctc gcgcagaaat tattcgagag
       61 tatgagctta cgccttcttc tttcgataaa tggatgaagc aagcacaatc aacgggctca
      121 ttcaaagaaa gagacaactt aacaccagaa caagcagaat tgatcgcact aagaaagaaa
      181 aataagcaac tcgaaatgga gaatgatatt ttaaagcaag cggcgctgat attcggacga
      241 aaagacaagt aattgatgcc aacaagcata aatattccat a
//
LOCUS       DANJLF010000259          276 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000259 DANJLF010000000
VERSION     DANJLF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.denovo.283"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
BASE COUNT          114 a           30 c           50 g           82 t
ORIGIN      
        1 gattccttta aggtgtcact aaaaattata aaaaatagtg ttgacgcact tttgatgtca
       61 tgttataata acgaagtcgt ttggatgaga tagctaataa agcttgattt tatcgaaaaa
      121 aacttcgaaa aagttattga caaataacaa taaagatggt atgataatca agtcgctgct
      181 aatgagtggc aaagacgttt agaaaaaata aacaaagaag ttgttgacat tctcttctga
      241 atctgataag attaaagagt tgttaaaaca aaaaga
//
LOCUS       DANJLF010000260        16933 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.051, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000260 DANJLF010000000
VERSION     DANJLF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(134..577)
                     /locus_tag="PWT25_002991"
     CDS             complement(134..577)
                     /locus_tag="PWT25_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL1022974.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="PWT25_002992"
     CDS             complement(795..2135)
                     /locus_tag="PWT25_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1022975.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="PWT25_002993"
     CDS             2404..3300
                     /locus_tag="PWT25_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL1022976.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="PWT25_002994"
     CDS             complement(3450..4391)
                     /locus_tag="PWT25_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL1022977.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="PWT25_002995"
     CDS             4517..5638
                     /locus_tag="PWT25_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL1022978.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="PWT25_002996"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="PWT25_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL1022979.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="PWT25_002997"
     CDS             7426..7992
                     /locus_tag="PWT25_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1022980.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="PWT25_002998"
     CDS             8203..9264
                     /locus_tag="PWT25_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL1022981.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="PWT25_002999"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="PWT25_002999"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL1022982.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="PWT25_003000"
     CDS             10177..12159
                     /locus_tag="PWT25_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1022983.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="PWT25_003001"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="PWT25_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL1022984.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="PWT25_003002"
     CDS             12881..14305
                     /locus_tag="PWT25_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1022985.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="PWT25_003003"
     CDS             complement(14486..15259)
                     /locus_tag="PWT25_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1022986.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="PWT25_003004"
     CDS             complement(15256..16161)
                     /locus_tag="PWT25_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1022987.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="PWT25_003005"
     CDS             complement(16315..16635)
                     /locus_tag="PWT25_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL1022988.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5463 a         3092 c         3476 g         4902 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tat
//
LOCUS       DANJLF010000261        15686 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.059, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000261 DANJLF010000000
VERSION     DANJLF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..585
                     /locus_tag="PWT25_003006"
     CDS             <1..585
                     /locus_tag="PWT25_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1022989.1"
                     /translation="NYVRSHGKKYTYELAEQFSKEKSGGQKADYPNPIAIPVNGGWRY
                     NYGNQFYVQLVSQYLTDTSPTEFDDETVQVIMDEALKYEGFPYVFGGASPTTSFDCSG
                     LIQWVYDKAGISLPRVAQDQYDATQEISMEEAQAGDLIFFHSTYNAGTYVTHVAIYLE
                     GNRFYHAGDPIGYGDLSSRYWQDHLIGARRVIHN"
     gene            602..1531
                     /locus_tag="PWT25_003007"
     CDS             602..1531
                     /locus_tag="PWT25_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1022990.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            1776..1892
                     /locus_tag="PWT25_003008"
     CDS             1776..1892
                     /locus_tag="PWT25_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL1022991.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            1908..2711
                     /locus_tag="PWT25_003009"
     CDS             1908..2711
                     /locus_tag="PWT25_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL1022992.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            2946..3182
                     /locus_tag="PWT25_003010"
     CDS             2946..3182
                     /locus_tag="PWT25_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1022993.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3189..5141
                     /locus_tag="PWT25_003011"
     CDS             3189..5141
                     /locus_tag="PWT25_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL1022994.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(5213..5710)
                     /gene="dfrG"
                     /locus_tag="PWT25_003012"
     CDS             complement(5213..5710)
                     /gene="dfrG"
                     /locus_tag="PWT25_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL1022995.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            6072..7112
                     /locus_tag="PWT25_003013"
     CDS             6072..7112
                     /locus_tag="PWT25_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL1022996.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            7210..7398
                     /locus_tag="PWT25_003014"
     CDS             7210..7398
                     /locus_tag="PWT25_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL1022997.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(7456..7809)
                     /locus_tag="PWT25_003015"
     CDS             complement(7456..7809)
                     /locus_tag="PWT25_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1022998.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            8340..8762
                     /locus_tag="PWT25_003016"
     CDS             8340..8762
                     /locus_tag="PWT25_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1022999.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            8759..8989
                     /locus_tag="PWT25_003017"
     CDS             8759..8989
                     /locus_tag="PWT25_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1023000.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            9487..9687
                     /locus_tag="PWT25_003018"
     CDS             9487..9687
                     /locus_tag="PWT25_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL1023001.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            9714..10907
                     /locus_tag="PWT25_003019"
     CDS             9714..10907
                     /locus_tag="PWT25_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1023002.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(10975..12540)
                     /gene="guaA"
                     /locus_tag="PWT25_003020"
     CDS             complement(10975..12540)
                     /gene="guaA"
                     /locus_tag="PWT25_003020"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL1023003.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(12599..13522)
                     /locus_tag="PWT25_003021"
     CDS             complement(12599..13522)
                     /locus_tag="PWT25_003021"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL1023004.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13718..14323
                     /locus_tag="PWT25_003022"
     CDS             13718..14323
                     /locus_tag="PWT25_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1023005.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            14631..15647
                     /locus_tag="PWT25_003023"
     CDS             14631..15647
                     /locus_tag="PWT25_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1023006.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
BASE COUNT         5269 a         2717 c         2982 g         4718 t
ORIGIN      
        1 aattatgtga ggagtcatgg gaaaaaatat acctatgaat tagccgaaca gttttctaaa
       61 gagaagtcgg gtggtcaaaa agcagactat cccaatccta tagccatacc tgtgaatggt
      121 ggttggcgat ataactatgg aaatcagttt tatgttcagt tggtttcaca atacctaacc
      181 gacacttccc caacagaatt tgatgatgaa accgtgcaag tcattatgga cgaagccttg
      241 aaatacgaag ggtttcccta tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt
      301 agtggcttaa tacaatgggt gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa
      361 gatcagtatg acgctacgca agaaatctct atggaagaag cccaagcagg cgaccttatc
      421 ttctttcatt caacctataa tgcaggcaca tatgtgactc atgtggcgat atatttagag
      481 ggaaatcgct tttatcatgc aggcgaccca atcggttatg gcgacttaag cagtcgttat
      541 tggcaagacc atctgattgg ggctagacgt gttattcata actaagaaag gaatgaatca
      601 aatgattata aaaatcgagc gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc
      661 gagcaagcaa agaaaagttc cgatgattaa agttggcacg cataagaaat taacctttgt
      721 tttatgggtt ttattgattg gtagtgtcgg atttggaatc tataaaaatt ttactgccat
      781 tgacactcat actgtccgtg aaacagaaat cattaaacag cagattgtcg ataccaacca
      841 agtagaaagt tttgtgaaat cctttgcgaa agactacttt tcgtggcaac aatcccaaga
      901 agccattgat aaacgaaatg aaaaattgac ccattatttg acggaagaat tgcaggtatt
      961 aaatgaagaa atgattcgta aggatattcc tactagttct agtgtgaacg atatacaggt
     1021 gtggcaggtt tctcaagtga atgagaacac ctttgaggta ctgttttctg ttgaacaagt
     1081 tattaccgaa gacaaagaca aggaaactat atcgtctagc tttcatgtgg ttgtccatat
     1141 agatgaatca gataatatgg ttattatcaa aaatccgaca atgagtaaga aacctcaaaa
     1201 atcggactac caacccaaac agctagaaag tgaccataca gtagatacag aaacgatgga
     1261 cgaaatcact agctttttag aaacgttctt ccagctctat cctaccgcca cagaaaaaga
     1321 attgacttac tatgtaagta atcatgtgtt acctatgatt aacaaggaat atgttttcga
     1381 ggaattggtg aatcctatct ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt
     1441 gaaatattta gatcaagaaa caaaggcaac ccaaatctcg caatttgagt tgatattaga
     1501 aaaacaagat aattggaaga ttgtaaaata acaaatattg gtacatgatt acagatactt
     1561 tgtaatcatg tactcttttt gataaaaaat tggagattcc tttacaaata tgctcttacg
     1621 tgctattatt taagtgacta tttaaaagga gttaataaat atgcggcaag gtattcttaa
     1681 ataaactgtc aatttgatag cgggaacaaa taattagatg tcctttttta ggagggctta
     1741 gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat ccagagaata
     1801 tctgtatgct ttgtatacct atggttatgc ataaaaatcc cagtgataaa agtatttatc
     1861 actgggattt ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta
     1921 atattggagt tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat
     1981 ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata
     2041 atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt
     2101 gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag
     2161 tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg
     2221 tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct
     2281 tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta
     2341 aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat cctaatatgt
     2401 gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga aacgatgacc
     2461 ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa
     2521 gcataagatt tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata
     2581 tagggattga taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag
     2641 gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc
     2701 ttgcatatta agtagttcag ttttggagta caaaaagtga aaatgtaaac ctaaatgagt
     2761 atcaaattgc aactgaattt gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa
     2821 tttacaccac aaaatgtaag ttaaaatgta tataaaaatg cgaacgtgaa ataattattg
     2881 taggaatgca aattggtgtg ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt
     2941 ttcctatgca atttcttgag ccaaaaaata agaacgctaa gtctgtggat tgggaaatat
     3001 cagagcaagt aagggtgata gttaagcaat acgctgaata tgcagagcgt actgaaagcg
     3061 aagcagtgga tgaattctta ttgaacattt tagacgataa gaagtttatt gaatggattg
     3121 ccaacaagcg aagtaataag cggattgttg aaaaaatggg tatcaaagat agagtgggtt
     3181 gatagacaat gaccaagctt aaaagattag ccacgaagga agatgaaatt gtagatgtta
     3241 aaattccgat ttcaaacgaa gaattaaaag atagggctaa acaatatgat ttattaactc
     3301 caaaacgatt tgcaacaagg tataacaaaa tgttgttctt gccaacgtct tttaaatgga
     3361 acggttctga atatcccatt caatataatt actgcatcaa tccgttttgt tgtaattttg
     3421 ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc aagtcgatat aaaatgactg
     3481 gtagttccaa agataaaggt cataaaggta tgtactgcaa tgacaatcct attggcagag
     3541 gtgtatcgca aaactgtaca gtaacccctt tatctaattg gtctgttgtt gaagaaatta
     3601 aacgccttat agaaatcaat agcatccaag atgttgagcc agattatcaa tttcataaag
     3661 aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg
     3721 ggaaaagtag gggcaaatct caacgctatc aatgtaaggc gtgtaagaag ttcactaatg
     3781 ttctaccaaa aagagaagaa accactactt atcaccaaca aaagaacacc attcttccaa
     3841 tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg tacttgtgat attttaggaa
     3901 ttggagttgg aacctactat cataagctgg agtggctgta taggcgttgt ttggagtttt
     3961 tagaacgata tgagacacaa ccccttcaaa caaagaaatt taacgagatg tggcttaata
     4021 cggacaagat gcactactac ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat
     4081 atacggggtt tgaagacctt aatatgcaaa cgtatatagt tgtatctgct gaggtcttat
     4141 caagatatgt gttccgttct gatgttgcct atgattggaa tatttcaatg gatgaattaa
     4201 atgaagatac aagaaaattc aaagaagacc atctcaatac ttttagtcgt aaaaatgata
     4261 ggttagattg gtcttattat ccacaagaac catccgctaa cgatagcgaa aataggaatg
     4321 cttatcttca cgaattaggg aaaattacta ataggagtag atttgtcgat ggtttaaatg
     4381 ttgatgctcc ttatactaca actgctcact attggttaat taaacaaatg gttaatgccg
     4441 acgaatggcg aatgattagt gatgatgact tctctattag aaacgccttt tatagagtat
     4501 tcacgaaaga attaaggttg agcgatgcac atcattttat ttgccaagtt aacaagacga
     4561 aaagcagaaa acaatgctta aaagaattcg gacaagcaaa agcagaactg ctcgattggg
     4621 gagatatcag aggattcaaa acaaagtttc tacggacttt ggcatctcat tatctaactg
     4681 aattactaac aagtcatcaa ttccacgaag aggctattag taaagatgga gaaagatatc
     4741 gcaaatatgc cgataaccct atcaaacatc cattagccac taaggataaa ggattttatt
     4801 cagttgattg tagaacggat ttatctgctc tggaaccaaa cgaaatcgca aagatgctat
     4861 taaatgttaa tgaccattcg accaatagct ttattcaaca gataagacgt tatatatcat
     4921 ctcttgaaag accattaaca acagctcgtg gtgataagaa gagctatatt tacgctaact
     4981 tcaatccgaa gtatgctcaa tttgcgatta caattctaag aacttactac aacttttgtc
     5041 gcccttttaa atcagcggat aaaaaggttt tgacacctgc tcaaagatta gggattacag
     5101 ataaacaatt tgattggaaa gatataattt atttcaaatg acaaaaagag cttggagtaa
     5161 tcccaagctc ttgtgcttta tctttactgt tcttcttcaa ttaacgcacc cgttaactca
     5221 ataagttttt tctttcatat acatgaaaat agtagttata cggattttta tcattcttta
     5281 tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga aaaaaagtat
     5341 ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa gggaaaaaca
     5401 aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta cataactcaa
     5461 aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc ttatctctcg
     5521 tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg ttcttcctac
     5581 ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc ttgggaatcc
     5641 tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca gcaatcaaag
     5701 aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc ttatttctat
     5761 caaaaaacct tttctcatta tcctgcccgt tagttgaata agcaattttt tctataagtt
     5821 atattatcat aataccacaa aatcacaaat tttcattcaa ttatcatttt caattgcatt
     5881 ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag tagtccattt
     5941 tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca tatatacgcc
     6001 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
     6061 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
     6121 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
     6181 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
     6241 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
     6301 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
     6361 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
     6421 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
     6481 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
     6541 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
     6601 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
     6661 atggttggaa tgtgacggat tgtaaaatct gttttaagta cggtttatac tatagccctg
     6721 ttagtactcc agcagatttt cggatgctta ctcctattgt actggagcaa gcctttagaa
     6781 aagctggaac agaattgtta gagccatatc ttagttttaa agtttatgca ccacaggaat
     6841 atctttcacg ggcatataac gatgctccca aatattgtgc aaatatcgta aatactcaac
     6901 tgaaaaataa tgaggtcatt attattggag aaattcctgc tcgatgtatt caagattatc
     6961 gcaatgattt aacttttttt acaaatgggc ttagtgtttg tttagcagag ctaaaaggat
     7021 atcaggttac cactggcgaa cctgtttgcc agacccgtcg tctaaatagt cggatagata
     7081 aagtaagata tatgttcaat aaaataactt agtgcgtttt atgttgttat ataaatatgg
     7141 tttcttatta aataagatga aatattcttt aatatagatt tgaattaaag tggaaaggag
     7201 gagattgtta ttataaacta caagtggata ttgtgtccta tttgtggaaa taaaacaaga
     7261 ctacgaatac gagtggatac tatacttaaa aatttccctt tatactgccc caaatgtaag
     7321 aacgaaactt taattaatgt tcaaaaaatg aatataataa caatcaaaga gccagacgcc
     7381 aagacgcaga gccgataatt tgagaaatga aactctcatc ttatcggctc tttttgttta
     7441 tctgaatttt actgactagc cttcaatatt tcttgcttca ttgatacctt ttgctagtgc
     7501 ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttttcta cctgtaacct
     7561 tcgagtgctt ttaatcaaat tatctgtagg taagaaaaat tcatcaatag atacatggag
     7621 taatgataca agatcataca gcacttgtgt acttgggtgt tgtcctttgt tttctatatt
     7681 tgttaaataa cgtgggtcaa tttcaatcat tgttcccact tgttcacgag tgagaccccg
     7741 tttcattcga gcctctttaa tcgctaaacc aacggctcta aaatcgtatt tatcttcttt
     7801 tttacgcata ctaaatcacc tctatacatt ttactgttcc tatataacca taaacaggta
     7861 tagaaaaaca tgttatatag tttatgagtt cctatagaac actgtggaat ttattaatgc
     7921 gatgaatcca atttcaaata gaaaggtcgc tgattttatt tacctataca actttataaa
     7981 atttagtcat actcctagaa agtagataaa attggcgact taatggaggg aaaagtcgcc
     8041 atgagaaaga taatctgtca tctagttctc ttaattaagt tcttatcaaa aaaacttatc
     8101 cacctgtact ggatagatat gaccatcaat gtggttttgc agattacata gatagaaaaa
     8161 tcaaagcata tgaacagaga aaacaatctg tttatgtgct tttttggtca cttaataaat
     8221 atcttttagg atttctcttc atttatgcaa caaatccccc gttcgtgtcg gggtttagta
     8281 tgaagggaga taaagaagca catgccactc cctttcacga cagaaagggg gtgaaaagca
     8341 tgaaaccatc ttcttttcaa gaaaccatag aaaatcagtt tgactatatc tgcaaacaag
     8401 ttattgaaga tgaacgaaaa gattacttca aacatctaga cagactatca gcaaaagaag
     8461 tctcgttttc tgatatagat aactaccttg tcaactcttt ttctactgtt gatacgtacg
     8521 tcactgattt ccaactattt aaattatttg gattaagaat caatgttgaa agtgatttat
     8581 taagtgaagc cctgcgtcat ctttcagaga aaaaacggaa cattatttta cttcattact
     8641 ttatggatat gagtgatgtg gagattgcgg agttactaaa tttgaatcgt tcgactgttt
     8701 atcgtcatcg aataagtgga ctagccatga ttaaagaatt catgaaggag tgtaaagaat
     8761 gaaaacagct tatccaaggg ttcccttccc cctcattgta aaggcgacag atggcgacgt
     8821 ggaagcaatt aatcagattg ttaaacatta tcgagggtat acctcaaaac gttcattacg
     8881 tcgcatgaca gacgaatatg gaaattctca tatggttatt gatgaaaccc tacgtggcag
     8941 aatggaaacg aaactcatct ctaagatttt atcgttcgaa attaaatagt cccatttttt
     9001 cgtggaagcg tgtcgaatcg tatccacgct tcccgatttg ggaggtttgt tattccatca
     9061 aagcatattg tgtgttcagt aggttttgat aggctaacaa gccaaacgtt ctttgacaac
     9121 caaataaagg caatcgaata cgtttcggta agaaaagagc tgatgaacta acacgccatg
     9181 acctacaaaa ttagtgagcg attcatgtta gtaattcgaa agtaatcttt agcaggattg
     9241 ctgccatgac tttcttatcg tgataatgat actcccgtat agccatagtc cgagcgttag
     9301 aagcgtcaca ggcaatgggt acggctacat gagaaccatg cagaagtgaa attcctgtga
     9361 gctttgctag agctgttcga ttgctaagat attcttctct tgtcagtgta ttattataaa
     9421 actatttgat ataattatag gtgaatattg gagttttcct agcctataat tgggaaagga
     9481 gtaataatga taacggtcac aaaaaaggat ttagttgagc taggatatgg aacttctttt
     9541 tcagcagaca tcataaaaaa agcaaaagaa ttgatgatta caaaagggca tatctactat
     9601 caatctcgaa agctagatag agtccctcgt gaagctgttg aagaaattct aggaattcgg
     9661 ttttcaaatg aaaacaggtc tgagtagttt ttgcacagta aggagtaaaa actatggtta
     9721 aagacccaat aaaaaaagca aagaatggaa cctattattt tcgtgcaaat ctaggttatg
     9781 attctaaagg gaaaaaaatt caaaaatatc gtagtggatt cactactaaa aaagaagcaa
     9841 gagaagaata ttcgaaactg ctattgatga aaccagaaga attaagtgag aataaggata
     9901 aaatgacgtt taaacattat atctttgaaa ttttccttcc atggtataag acacaggtaa
     9961 agctcagaac atatgaaaat cgcttaccaa ctattaagaa gcatttctct tattttgata
    10021 aaatggctgt ttccgatatt gagcctattg atgtgcaaaa ttggcaacta aaattatcaa
    10081 agaagtgtaa gtcttcttat gtaagagctg ttcaaggact attttcagtt gctatggatc
    10141 gagcaattgt ccttgggata accaccacaa atccatctaa gattattggc aatgtgaaaa
    10201 agcaaaagtc taaaattgaa ttttggacaa aagaagaatt tgaaaaagta atctctctaa
    10261 tttacaaaga agattactat caacactttt tatttatctc tctgtggttt ttatttatga
    10321 ctggaatgcg aattggtgaa gctactgcca ttcaatggga agatattgat tttgactcag
    10381 gagtactaac catcaacaaa acactctatt acaagaatca atcgaattat agatttgttg
    10441 aaccaaaaac aaaggcgagc gttcgtcata ttgtattaga taaatgcaca ttaacttatt
    10501 tatccgaatg gaaggaagta caacaaaatc taattcagac agactttgta atgagctata
    10561 acggtattcc tacccaaaaa catacactat catatgctat tgaacgctat gctaagctgg
    10621 ctggtatcca tcgaatcaga atccatgggt tacggcattc tcatgcttcc ttgttaatca
    10681 gtatgggaga aaatccactt atcattaaag atcgtctagg acacgaagat attgagacga
    10741 ctttaggaac gtatggacat ctttatccta acagtaattt cgaggtagct aacaaattaa
    10801 atggagccat ttcctttaaa gaagcaaaac aaaattatga tacttcacca aagaatcaat
    10861 ttactgtaga ctacttgcga aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa
    10921 agataagaag agccagaaac ccttggtata agaggatttc tagctctcct acaactattc
    10981 ccactccact gttgctggtg gtttagaagt gatgtcatac acgatacggt tgacgtgggc
    11041 tacttcgttt acgatacgta cagagatttt ttgcaagact tcccatggga tacgggcaaa
    11101 gtcagcggtc attccgtcga ttgaagttac agcacggata ccgactgtgt agtcgtaagt
    11161 acgtccgtca cccataacac cgacagagcg gatccctgga agaacagtga aatattgcca
    11221 gatatcacgg tcaagtccag cagctgcgat ttcttcacgc aagatagcat cagattcgcg
    11281 tacgatttct agcttgtctt cagtgatttc gccaagaaca cgaataccaa gaccaggacc
    11341 agggaatggc tgacgccaaa cgattgaatc aggcatgcct aactgagtcc ctaattcacg
    11401 gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg
    11461 cagtccacct acattatggt gagatttgat cgtctgtgct gtttctgtac cagattcgat
    11521 aacgtcagta tacaacgttc cttgtgctag gaaagagacg ccttcttcac cagcaagttt
    11581 tgttgcttcg tcatcaaaaa catatacgaa ttcattccca atgattttac gtttttgttc
    11641 tgggtcagat acacctttca acttgtctaa gaagcgatct cttgcatcga ctttgatgat
    11701 gttcaagccg aattttccgc ctagactttc cattacttgt tccgcttcgc ctttgcgtag
    11761 cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac
    11821 cccaacgaca ctagaatcaa cgccacctga aagaccaagc aagacttttt tgtcaccaac
    11881 ttgttcacgg attttttcta cttgcatatc gataaagtta tccattgtcc agttaccttc
    11941 acagtgacaa acatcaaagg caaagtggcg cagcaattcg attccgtatt cggaatgacg
    12001 tacttctgca tggaattgga taccataaaa attacgtgtt gtgtcttgga tagaagcaat
    12061 cggacaatct ttgcttgttc cgactgtttc aaagccttca ggaactttag ttaccaagtc
    12121 accatggctc atccatactg tttgttgttt tggtgtttct ttaaacaaga cagcttgatc
    12181 gcttgttact tctaacaagg ctttcccata ttcgcggttt ttagcaggct ctacggaacc
    12241 gccaagtttg tacgtgatca attgcatacc atagcaaatc cctaatacag gaatacctaa
    12301 attgaagatc tcaggatcga tcccaaatgc attctcatca tatacactgt ttggtccgcc
    12361 agaaagaatg attccctttg gattcatcgc ttttacttct tctgcagtaa tgcggtggct
    12421 caataattcg gaaaagactc caagatcacg aatacgtctt gtgatcaatt ggttatactg
    12481 actaccgtag tctaatacga taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa
    12541 atttattacc cctatccctt taaattttat ctcaaaatag tcaaactatt ttaaaatgct
    12601 aatattttcg catgaaaatc gaatcgatga tatgattttc agttttatgg agaattatat
    12661 cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc acatttttcc
    12721 atacatcttt tgccatttta aaggcttctt cacgatctcc gatagcaaaa cgataataat
    12781 aattctctgg attttggaaa gccgtatcca gtaacgcacc aaaacgatcc aaataccatt
    12841 tttcgatcag cacgggatcg gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt
    12901 agatttgctg attcgcaggt aattgaagcg tattgatccc ctcaacgatc aagatatcag
    12961 gctgctggat caattcgtac ttgtctggaa tgatatcata cacatcatgt gagtaaacag
    13021 gtacttttat ctcttcctga ccagatttta cacggttcaa gaaatcgatc agcatctcca
    13081 tatcataact ttcaggaaat cctttacggt ccatgattcc acgctctttt aatatacgat
    13141 ttggataaag aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag
    13201 acaaaatcgt ctgcagcagt ctggctgttg tactttttcc aacggctaca cttccggcga
    13261 tcccgataat aaaaggcggt acttctacgt atttatgaag aaataaacct ttactcaaga
    13321 acaaagattc aaattctttc atataaagat aaatcagatg agcaagaggt acatagatat
    13381 ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt gatattatct agttcatctt
    13441 gtgtcaaagg tgcttgccca ttacgataaa attctcgcca ttcttctctt gccacacggt
    13501 agtagttcat tggatcattc atattagcct cctgcgttct cccatcaatg gaactatttt
    13561 aacacaaaat aaacgcaaac cccaccactt aagagagatt gccgatcttt ttgtataatc
    13621 tttataatag aaagaaacgg agtcttattc ttgacttgcg cacggataca taaaaggata
    13681 gactgttgtc atgaaagaaa tgagaaggat ggaagacatg acaaatcatt actattctga
    13741 acgcccagat acggcgcacg attttgaaca atgggctttt gagttaaaag gcagaacctt
    13801 tcaatttgta acagattctg gtgtattttc acgtgaaaca gtggattatg gttcacgtgt
    13861 cctgattgat gctttcgatt gggaagagtt acctgatgaa ggaaagatct tagatgtggg
    13921 atgcgggtat ggaccgattg ggttagcttt ggcatttgcg acacagcggt ttgttgaaat
    13981 ggtcgacatt aatgaacgag cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat
    14041 cgaacatgtt gatatccatc aatcgaatat ctatgaagca gttcatgaag aaacctatgc
    14101 agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac
    14161 aggcgcatat cctcggttga agaagggcgg tacactgaca attgtgatcc agaagaaaca
    14221 aggagcgccg agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag aaatcgtcac
    14281 aaaagataaa ggatattata tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg
    14341 gagcgacgaa agagtggaac tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt
    14401 tgagaagttt ttaaaaacaa agaactattt tttcttcaaa aagaaatagt aggaacagag
    14461 tcgttgtttt ttacgataaa ataaataaga tacagaactt gtatagaagg ctgccaagtc
    14521 ggtagataaa ggaggttttt atcaaaaaac atactttcct tttttctcta ttcttttctt
    14581 gggggttttg gtacactgat agggaattta gatagaaggg agccaatagg atgaagctga
    14641 ccatcagaga tatcgcagaa atggcaggcg tatcagtaac aactgtctca cagattttga
    14701 ataacaaagg aagcagattt agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg
    14761 aacatcatta taagcccgat tatttcgctt caaatgtgat caatcgacat tcaaagacca
    14821 tcggaatgat cgtccctgat gtcactgatt tcttcttctc caaactgatc gaaggtgtag
    14881 agagctattt gaatcctttg ggctatgtga tcatgttatg caattctcgt catagccaag
    14941 aaaatgagat caagtattta caagagcttt cccaccgctc agtggacgga atcttgctgg
    15001 ccacaccaaa tattttacct gagaaatatg cattagacag cggtttttat caaaaaatgc
    15061 caatcatttt aatcgatcgc ggattgaatc gtcgggataa cggacggctt atcgtaaagg
    15121 aatatgaggg cgcctatcaa gctgtttcgc tgtttattga aaatggccat acgaagatcg
    15181 gcatgctgaa ggaaaccact ggatactatc aattagaaga acgtttcaac ggatatcgtc
    15241 atgcgctgaa agatcatggc ctgcctttta atgggaaatt tgttgaacat ggagagttga
    15301 ctgtccaagg tggatatact gcatcaaaaa aattactcaa acataaagat atcacggcta
    15361 ttttttgcgg gaatgatgcc atggcgatcg gatgctatca ggcgatcgat gaattgggca
    15421 aaaaaatccc agaagatatc tcagtcatag gattcgatgg attaaagctg tcagaataca
    15481 tgattccgca attgacgaca gtgcagcagc caagttttga tattggattt tatgctgcac
    15541 gtttcctgat cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa
    15601 caaaattaat tattcgtgag agcgtaaaag tattgacaaa gggctaactc ggcggtatat
    15661 tgcttatgta agcatttaca aaggct
//
LOCUS       DANJLF010000262        13213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.069, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000262 DANJLF010000000
VERSION     DANJLF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(204..2162)
                     /locus_tag="PWT25_003024"
     CDS             complement(204..2162)
                     /locus_tag="PWT25_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1023007.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="PWT25_003025"
     CDS             complement(2159..3031)
                     /locus_tag="PWT25_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1023008.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="PWT25_003026"
     CDS             complement(3373..4245)
                     /locus_tag="PWT25_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1023009.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="PWT25_003027"
     CDS             4373..4942
                     /locus_tag="PWT25_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL1023010.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="PWT25_003028"
     CDS             5009..5746
                     /locus_tag="PWT25_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1023011.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="PWT25_003029"
     CDS             5977..7245
                     /locus_tag="PWT25_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL1023012.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="PWT25_003030"
     CDS             7732..8196
                     /locus_tag="PWT25_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1023013.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="PWT25_003031"
     CDS             8180..8809
                     /locus_tag="PWT25_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1023014.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="PWT25_003032"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="PWT25_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL1023015.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="PWT25_003033"
     CDS             10688..11134
                     /locus_tag="PWT25_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL1023016.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="PWT25_003034"
     CDS             complement(11333..11848)
                     /locus_tag="PWT25_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1023017.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="PWT25_003035"
     CDS             11965..13167
                     /locus_tag="PWT25_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1023018.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DANJLF010000263        13135 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.072, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000263 DANJLF010000000
VERSION     DANJLF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(73..270)
                     /locus_tag="PWT25_003036"
     CDS             complement(73..270)
                     /locus_tag="PWT25_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1023019.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(365..2125)
                     /gene="efrB"
                     /locus_tag="PWT25_003037"
     CDS             complement(365..2125)
                     /gene="efrB"
                     /locus_tag="PWT25_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL1023020.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(2122..3852)
                     /gene="efrA"
                     /locus_tag="PWT25_003038"
     CDS             complement(2122..3852)
                     /gene="efrA"
                     /locus_tag="PWT25_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL1023021.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            4245..4457
                     /locus_tag="PWT25_003039"
     CDS             4245..4457
                     /locus_tag="PWT25_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL1023022.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            4459..6138
                     /locus_tag="PWT25_003040"
     CDS             4459..6138
                     /locus_tag="PWT25_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL1023023.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(6392..6592)
                     /locus_tag="PWT25_003041"
     CDS             complement(6392..6592)
                     /locus_tag="PWT25_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1023024.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(6742..6885)
                     /locus_tag="PWT25_003042"
                     /pseudo
     CDS             complement(6742..6885)
                     /locus_tag="PWT25_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(7120..7782)
                     /locus_tag="PWT25_003043"
     CDS             complement(7120..7782)
                     /locus_tag="PWT25_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1023025.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            8174..8233
                     /locus_tag="PWT25_003044"
     CDS             8174..8233
                     /locus_tag="PWT25_003044"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDL1023026.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            8291..9022
                     /gene="erm(A)"
                     /locus_tag="PWT25_003045"
     CDS             8291..9022
                     /gene="erm(A)"
                     /locus_tag="PWT25_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDL1023027.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(9148..9930)
                     /locus_tag="PWT25_003046"
     CDS             complement(9148..9930)
                     /locus_tag="PWT25_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDL1023028.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(10084..10461)
                     /locus_tag="PWT25_003047"
     CDS             complement(10084..10461)
                     /locus_tag="PWT25_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1023029.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(10468..12360)
                     /locus_tag="PWT25_003048"
     CDS             complement(10468..12360)
                     /locus_tag="PWT25_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1023030.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            complement(12357..12917)
                     /locus_tag="PWT25_003049"
     CDS             complement(12357..12917)
                     /locus_tag="PWT25_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1023031.1"
                     /translation="MIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIHL
                     TPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFGK
                     PLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTTL
                     NTYVHLSDQDMKNEFNKYLERKEHKK"
BASE COUNT         4076 a         2645 c         2071 g         4343 t
ORIGIN      
        1 aaaaaaacag gtaaaacctg ctcaggccag atgtctattt acagtatctg gtaactgacg
       61 ttttacctgt ttttattgta catctatatt ttcttctatt acttttttta aaattaattc
      121 gtacacttct atttgtgctg ccaagcgttc gatttgaaac tcattttcaa aaaaatcagc
      181 tgaatcaaaa ggcttcgtct gacagacgtg cgttaaacga tcaacttctc ttttggcttc
      241 agtaattttt tcatttaaaa actgttccat ctccgtgtcc tcacttttct tgataacttt
      301 actttaccat tatcaagaat agagaacaaa aaataagttt taaactgttt tattcttata
      361 tgacttattc tgcttcctca gcaaattggc tgttatacag tttttcataa aatccacctt
      421 gtgccaataa actctcatgc gttccttttt caatgatctg accttgatcc atcactagaa
      481 tcagatctgc atcacggatt gtcgataaac ggtgagcgat gacgaaactt gtccgtccct
      541 ccatcacgcg atccattgct ttttgaatca acgcttctaa acgggtgtct actgaacttg
      601 ttgcttcatc taaaatcaag attttaggat cggagaccac tgctcgggca atcgtcagca
      661 actgtttttg tcctaaagac acattgtctc cctcagaatt gatggccata tcatatccat
      721 ctggcatcgt tcggataaaa tgatctacgt ttgctgtttt cgcagcatca actacttcat
      781 aatctgtcgc atcaagcttt ccgaagcgga tattatctgc aatcgttcct tgataaagcc
      841 atgcatcttg caagaccata ccaaatagcg aacgcacatc actgcgactc attgatttgg
      901 tgtcgattcc atcaatcctt attgctcctt tgtctacgtc ataaaaacgc atcaatagat
      961 tgatcaatgt cgtcttgccg gcaccggtcg gaccgaccac agctacggtt tgaccagctt
     1021 tcacttcaaa attcaaatct tggattaatg gtttcttcgg atcatatgca aaatcgacat
     1081 gatcgaatgt cacatctcct aaaatcgttt ctggcagtga agcatctact ttgttttctt
     1141 tttcttctgg ctcatctaaa atctcgaata cacgtttcgt tgccgctgat gcactttgca
     1201 aaactgaaga caattgtgtc acgttcccca ttggctgact gatttgccaa atatattgga
     1261 tgaatgcttg tagttgaccg acaacgatcg ccccagtaat aacataaaag cttcctagaa
     1321 cagccatacc aatataagta gcataggcag ttaattgaac tagaggcatc atcaagccag
     1381 aaatgaatgc tgcacgaaac ccaaagtatt tcaagctgtg attaactttt ttgaatcctt
     1441 ccaatgtttc tttttcgcgt ccatatactt tcaatacact aaaacctgtc atattttctt
     1501 ggacgtagcc gtttagctca ccaagagaat tctgcatgct ttgaaaatat ttctgcgaag
     1561 cattgatcac tcgttttgag atccagatcg atgttggaat catgatcata gagatcagcg
     1621 ccatcatcgg ctggatataa aacatcattg ctgcagccaa cgtaattccc aatatcgcat
     1681 ttacgatacc aataaaagct tgttgtaaag caccgctgac tgcatctaca tcattcgtca
     1741 cacgagacaa aatatttcct tgctggtttt tatcgaaata tgaaaccgga agacgattga
     1801 ttttttcttc tatgtctctt ctcaaatcat gcatggcact ttgaacgaca tttgtcatca
     1861 aaacaccaga aagtaattgc gaaatacaat accctattcc aacaactgtg atccaaatga
     1921 gacatttgaa tatataagaa aaattgattg attcgccatt ggcaatatta cggctgattt
     1981 ctgtagtagg aagtcctgta acatatggca aagccacgtt gaaagccact gtcagaactg
     2041 taaataggat cactaaaaga aaagtctcac gatacggtct gatatatcga gctaaacgtt
     2101 ttaaggaaga aaaggttttt ttcatgctaa ttcctccttt gataattgag aagcggcaat
     2161 atcataataa atcgggcatt tttccaacaa ctcccgatgc gtccccatac cgacgacttc
     2221 tccttcattt aatacgatga tcttatctgc atgcatgatc gttcccacac gttgtgctac
     2281 gatcaataca gtagattctg tcgtttcttt tttcagacga gcacgtaact tcgcatccgt
     2341 ttgataatct aatgcagaaa agctgtcatc aaaaatatag atttccggac ggcgtatgat
     2401 ggctctggct attgccaatc gttgtttttg tccacctgaa aaattggaac caccttctga
     2461 taaatgttca ttaaatccat ctggttttcg agaaatgaat tctgaagctt gagcaatctc
     2521 agctgcacgt tccatttctt cttgcgtagc atcttctttc ccgtatcgca aattatctgc
     2581 aatcgttcca gtaaatagca gcgctttttg cggaataaac ccaatcttat ctcgcaactt
     2641 gcttagccga taatctctca cgtctactcc atcgatcaag atctttcctt cactcacatc
     2701 ataaaagcga gggatcaatt ggatcaacgt agattttccg ctaccggtac ttccaataaa
     2761 agcaacggtc tctcctggag aggctgtaaa actgacattt cgtatcactg gctcttgcga
     2821 atggcctgga taagcaaatg ttacattttg aaattcgata taccctttag tttttgtatc
     2881 agatatacca tttggatctt ctacaatcga aggtttggca tctagggctt cttggatacg
     2941 tccagctgaa acagctgctc ttggatacat catgaacaca ctagcaaata acatgaatga
     3001 gaacaaggca tggaatatat actcgataaa cgcaatcaaa tctcctacac gaagctcgcc
     3061 tgcatcgatt tgcactgccc cgctccagat gatcaatacc attacgatat taaataagaa
     3121 ataaaaaccc ggttgtgcaa cagccatcaa acggaataag ctttttgaac tagaagcata
     3181 attctcattg actacagcaa aacgtttttc ttcaaacttt tcattgacga atgcacgaat
     3241 cacacgcaat cctgaaagat tttcccgcaa gatactgttg attttatcta agtttttttg
     3301 ttgcttttta gataacggtt cagacacctt agcaatcgct acgactgcca ataatagtaa
     3361 aggtaatgca cctactacat acaaactgag tgtcacactt gtacgcatga ccatgtacaa
     3421 actgacaatg aacatcattg gtgccataaa accgatacgt aaaatatttt gcaggaacaa
     3481 catgatctga taagcatcgt ttgttgtacg agtgatcaat gaagatacgc ctagattttc
     3541 gtattcctga tgcgaataac tttggatctt ttcgaacaaa tcatctcgaa tatctgctac
     3601 gatattagtc gtaatcctcg aaccaaaata acctaacaag atattcaaaa taataccgat
     3661 tacagtaata acgatcataa atatcccttg ttgcttcaca taatcaaaat catcattgcg
     3721 gattcctaca tcgatcatac gtgctaaaat cgtaggaagt cctaattcaa tcaaaataaa
     3781 accaaatacg catatgaaat tgaaaaacaa gaatttctta taacgcatgg tataatgcca
     3841 cattagtttc actttctcat ctccttttaa tcttttgtta cattttcatt actaaaaatg
     3901 atagcacaac gagcctatta atagaacact tctctcataa aaaagtcatt atgattatag
     3961 cgggatgata aacattgatt ttttctttca atctagttga tttctatact tatttgctgt
     4021 tttatgctag aataacaatt aattgttgta tattttttat acagaaggta cggttgaaaa
     4081 aatatccgaa cagataagca aaaacgaggc ttgtctcttt gactcgaatc cagtattcta
     4141 gtcaaagcat atgaaaaacc aggtggcttg tcccctcgtt ttgtgttgtc taaaaaaatc
     4201 aactagctaa atcactgctt tacgcagata aaggagacaa aacaatgatc tataaagttt
     4261 attatcaaga aacaaaagta cgcaacccta aacgggaaga aacaaaatct ctttacattg
     4321 aggcaaaaag cgatgtagac gctcgccaac gtgtggaaga aaatacccca tacaatattg
     4381 aattcgttca acttctagaa ggaagtcatc tagagtacga aaaagaaaat gctgatttct
     4441 cattagtgga gttttaatat gaaactttcc atcaaaaata acgagacagg cgtctttgct
     4501 attggtggat taggtgaaat cgggaaaaat acgtatgggg tacaattcca agatgaaatc
     4561 attttgattg atgccgggat caaattcccg gaagacgatt tacttggaat tgactatgtc
     4621 attcccgact acagctacat catacaaaac ttacacaaag taaaagcctt ggtgattact
     4681 cacgggcacg aagaccacat cggcggaata ccctacttgc tgaaacaagc aaatattcct
     4741 atctatgctg gtcctctcgc attagcgttg atcacaaaca aattagatga gcatggcttg
     4801 cttcgagatg cagagctcca tgagatcaat gaagatacga tcatccgttt tagaaagact
     4861 tctgtcagct ttttcagaac gacccatagt atcccagatg cattagggat cgtggtgaaa
     4921 acaccttctg gaaatatcgt agcgactgga gacttcaaat tcgatttcac tcctgttggc
     4981 gaaccagcaa acttgcataa aatggctcgt atcggggaag aaggagtctt gtgcctttta
     5041 tctgatagta caaatgctga gattccaaca tttactaaat cagaaaaaac gattgggcat
     5101 tctattttga agatcttttc aaaaatcgaa ggacgtatca tttttgccag ctttgcttct
     5161 aacatcttcc gtttgcagca agcagcagat gccgctgtgg caactggtcg taaaatcgct
     5221 gttttcggac gaagcatgga aaatgccatc gtcaacggtc aacgtttagg atatatcaaa
     5281 gttccagacg gcacatttgt agatgcacac gaaatcaatc gcttgcctgc caatgaaacg
     5341 atgatcctat gtacaggttc tcaaggagaa gcgatggccg cgttgagccg catcgcgaat
     5401 ggtactcacc gtcaaatcca aatccagcca gacgatacag tgatcttttc aagttcacct
     5461 attcctggta atacaacaag tgtcaatcat ttgatcaatt tgctgtctga agctggtgca
     5521 gaagtgattc acggaaaaat caataacatc catacttccg gtcacggtgg acaagaagaa
     5581 caaaaattaa tgcttcgttt gatgaagcca aaatatttta ttcctgtcca cggagaattc
     5641 cgcatgttga aagtacatgc ttctctagca caagatgtag gtgttcctgc agaaaactgc
     5701 ttcatcatgg aaaatggcga tgttttagcg ttaactagcg attctgcgcg tcctgctggg
     5761 cattttgtgg ctggagatgt gtatatcgat ggtagcggga tcggtgatat tggtaatgtc
     5821 gttttacgtg accgtcacct gctttcagaa gaaggtcttg tattagctgt cgcgacagtg
     5881 gatatcaaga aaaaagaaat cttggctggt ccagatattc tctctcgtgg atttgtctac
     5941 atgcgagaat caggagaaat gatcaatgaa gctcagcgaa tcttattcca cgctttaaga
     6001 gaatgtttga atggacctga ctgttcagaa tataaattga aagaagcgat gactggcgcg
     6061 cttcagcctt tcttattcga acgtacagaa agacatccaa tgattttgcc aatggtaatg
     6121 acaccagatg aagaataaat aagaggttat gaaatcagca ccttgtcctg aatataagag
     6181 aaaacaaatg aaaaacatga gaagccattt ttgtttgttc ttattccttg atacaaggtg
     6241 gctctttcat agcctcttaa atttatttac tttttcatac aaaaaaatac ctgccacaag
     6301 agaaattgtc tcttgtggca ggtatttttg ttactcgctt tgttctctcg taaaaattga
     6361 tgtctaccat caatttgatt tacagataaa attaagcttt tacaacgttt gcagcttgtg
     6421 ggccgcggtc tgattcttca acgtcgaaag tcactgcttg accttcttct aatgttttga
     6481 atccgtcacc ttggatagct gagaagtgaa cgaatacgtc gcttccatct tcacgtgaga
     6541 taaatccaaa tcctttttca gcgttgaacc attttactgt tccgttttcc ataataatat
     6601 cctcctcgtg ccaaaagcac taatttaaat tgtaacattg ctcgtgtacg atagaggtgc
     6661 aacagatgtt tcaacttcta ttcccaaaca aaattacaat ataattgtac acttgcttca
     6721 ttttttttgc aagaaagcaa cttacttctc ttccatcatt ccttcttctc tcaaactaaa
     6781 atatcgtttg cgtccgatga tcaagtggtc cagtacagtg atccccatca tttctccaca
     6841 cttttgtatc cttttggtaa aattcatatc ctgctgcgat ggaatttcat attatcaaga
     6901 gtaaattaca aaagaatcat ttaaatcaat actttcactt tgaatacatg tatttgaagt
     6961 ggaaggtact tatttcaaaa atagtaaaac ctgtatctta aattacttaa tagtaacata
     7021 agatacaggc tgatttttta ttcattgtta tttatactaa agcacccgat agctctgaaa
     7081 acaatcacaa attcaacttt tcaaagccac agctttaagt tattttgtcc agacaacccc
     7141 cattgcccaa cccattttat ggaattggca tccaggcaac aacttttcat ataaatcgta
     7201 ataattttgt tcagataggt acttatctga agctaaatgc tcaagccatg atttagatgt
     7261 gttgtgatta taaattctaa tcgcattttt tattccaaat ttgaaacaat gcggtaaaaa
     7321 ctcttggatt gcccctaatt tataaacata ggtaggcggt gtctcgactt cagatacatt
     7381 atcaagaata actattcttc cttcctcatt caacagttcc ttcatttgct gtattacgct
     7441 ggctatatca tccaaatgat gaaaggttgt tcggcttaca ataaaatcaa acttctcatt
     7501 aaaattaagt tgttctgcat tcatattcag atagaccgta tttgttagtt gacgtttaga
     7561 tttggcaaga tcgagcattt gattagaaat atcaatccct accacttcat cataataact
     7621 tgctaatttc tccactaaca aacccgagcc acatccgata tctaatgctc tgcctttctt
     7681 tggagacata ttagacacaa agaatgaata atcattcaaa agctcattca cgaaatcgta
     7741 atcttctgca accttatcaa actgtgattc tattgtattc aaaaagatcc cccattccta
     7801 ctttatcgac attctttcat tacttaccac tttagatgtt ttttcgttgg ggataaaact
     7861 tccctttaga caattttatc caaagacaat acaacagtgc aactttatta aagtcactgt
     7921 cctttatcgc agcctttact ttttagtaaa gacagtggct tctcttatca agtttcaaaa
     7981 catattattt tgaagaaaac gtccatctga agtgtcaagt gcaaaattac atataaaggt
     8041 ttattctaaa atgaaaagat gatacaatca tattcagtta cataaggagg tttcaattat
     8101 gtgcaccagt atcgcagtag tagaaattac tttatctcat tcataatgaa aaaaatggaa
     8161 aggagataaa agtatgggta ctttttctat atttgttatt aataaagttc gttatcaacc
     8221 aaatcaaaat taattggtta taatgaacgc ttaatgtcag ttcattataa ccagtaagga
     8281 gaaggttata atgaaccaga aaaaccctaa agacacgcaa aattttatta cttctaaaaa
     8341 gcatgtaaaa gaaatattga atcacacgaa tatcagtaaa caagacaacg taatagaaat
     8401 cggatcagga aaaggacatt ttaccaaaga gctagtcaaa atgagtcgat cagttactgc
     8461 tatagaaatt gatggaggct tatgtcaagt gactaaagaa gcggtaaacc cctctgagaa
     8521 tataaaagtg attcaaacgg atattctaaa attttccttc ccaaaacata taaactataa
     8581 gatatatggt aatattcctt ataacatcag tacggatatt gtcaaaagaa ttacctttga
     8641 aagtcaggct aaatatagct atcttatcgt tgagaaggga tttgcgaaaa gattgcaaaa
     8701 tctgcaacga gctttgggtt tactattaat ggtggagatg gatataaaaa tgctcaaaaa
     8761 agtaccacca ctatattttc atcctaagcc aagtgtagac tctgtattga ttgttcttga
     8821 acgacatcaa ccattgattt caaagaagga ctacaaaaag tatcgatctt ttgtttataa
     8881 gtgggtaaac cgtgaatatc gtgttctttt cactaaaaac caattccgac aggctttgaa
     8941 gcatgcaaat gtcactaata ttaataaact atcgaaggaa caatttcttt ctattttcaa
     9001 tagttacaaa ttgtttcact aaattaaagt aataaagcgt tctctaattt cacaagagga
     9061 cgctttattc ttcccaaaaa ttgttcaata tttatcaata aatcagtagt tttaaaagta
     9121 agcacctgtt attgcaataa aattagccta attgagagaa gtttctatag aatttttcat
     9181 atacttaacg agtgctttca cctttgaata tagtccttcc cacttatcat cacactctcc
     9241 ccgatagcct tttctagcta tatccagtaa agttacatgc tctttaggta aaagaggtat
     9301 agcccattct gcagcgacat ctttcgaggt aatttcacca gtagtcactg tttgccacat
     9361 tcgagctagg gttaaaatta cattacgctc atcacctttt atcccctcaa ttagttctgg
     9421 caaagaatcc ttaattgctc ttcgaatatc tgtcaaaggt acggagacaa gtatacttga
     9481 agaatcagga ccaaatagag aaatactatt ctttcttgct tgtgctaaaa caatagccaa
     9541 atcaggatca tagcttggtt cctgaatttg tccattctca aattcacccc tgagccactc
     9601 accgtatata aattctcttt ttggaggata ttgccaaggg acaacttcac tcctatttat
     9661 aaccgtaact tcaagtggtc taacagaatc cgtatttcca atctttcctg atatagtcat
     9721 tagtctttct gttagttttt ttcgagttaa ttgaggtaaa ctatgattca cgacgactag
     9781 aacatctaca tcgctgttaa tgcgtaaacc accatttact gctgaaccaa atagatatac
     9841 tccaactatt gaacttccaa ataaatcttt tacgattttt aatgtttgaa tcgcttgatt
     9901 tggtattttt ccgttaatca aattgctcat gatttcacct cgtaactgat tatgttcata
     9961 taaagtttat attgatactc aatttactta ccctagattg gacatatacc taaatgactg
    10021 ttcaataaag ctgaccgtta gcgtttaagt acatcctttc acaatttgtc tacagattaa
    10081 taattattct ttattataca gatctccata taatttttga atttggttct gtaatttttt
    10141 attttctttt tctaattcca ttactcttct ttttaaggtt ttaataagga tttcctccga
    10201 acgagaactt ttcttgggtt ttgagactac atttgctgtt atttgacgct cacgaaggga
    10261 ttcgattctt tgcctaatat cgtgttcctt ataaagccat gatttagaaa cattagcttc
    10321 ctttgctatt gaattaaaat taataacttt accttcaatc gaaaatttag aaatcgcttt
    10381 gtctactttt tcccttgtct tttttgattt ctgcttcgcc aaacgtacaa tttctgttgt
    10441 atttctaact tgtttatcca ttgataatta ccccgtcaaa cttccaatga tttgttctaa
    10501 acgctcttta acacggctat tagtctctac ttgtctttgc cattgtttat ccttagctat
    10561 ggctaataac tcttctgtac gctctaactg ttcttcgtgc tgtggtaaga attgcttact
    10621 ggtacagaag tgagtgcaat ctaagcatgc attcgcatgt ggacaaccac ctgctactac
    10681 tggcaatcta caataaccat ttggaagcac ttgtgcattt atatttttct tgaaccattg
    10741 aagctctaca tcatcgactt cattatcttc atctagatca agcacatctc cattattggt
    10801 aaccagtttt tcctgaaatt tagtaaattc attttttaga gtttcatcaa agatatgagc
    10861 gtatctgctt gtcatttctg ggctttcatg ccccaaaaat ttctgcacaa tatgctgggg
    10921 catcccgttg ttaatcattc ttgttcctac tgtatggcga aaggcatggg catggaatct
    10981 ataaatctca cctgatttat ccactatatt ttgctcataa gctaatttat ttaactcacc
    11041 tctaaatgtt tcttgtttta atggcgatcc atcttttctt ggaaagaggt attcactatc
    11101 tggaaattcc tctgaaactt tatcttcccg aactttaata agtaaagcta cctctttaga
    11161 tattggaact atatgctcct ttttcatttt ccattgataa tactttaaaa agaaatctcc
    11221 atctttgtcc tctaatagac agcctttttt caaggtgcac aattcactta tcctcattcc
    11281 acattcttga acaatcatag tcatcgtagc tatatattcg ggtaatttat caagatgact
    11341 gttcaattgc tctaggacga attcatctat aaagcgtggt tttgctcttg gtattttcgg
    11401 atagtcctca gaataaatta atattttgga aggaacatca tcccattcta gcctaagaag
    11461 ggtactaaat agtccttcca atatagagat cctcccagtt attgtactag gttttattcc
    11521 catcatgttt agttcactta aatatgcttc aatttccact ctcgttaatt ggtgtactct
    11581 ctgaacttgt ttaaatttca tgtccagaaa attaaagaac tctttaagtc tttgggcaat
    11641 atcacttaca taggaaaagc tatccatgtt caatctcaac ttacaatatc tttttacaag
    11701 ttgtttaaaa tatgtattcc gaaacccttt aaagttaatt gtatattcat attgtgttgg
    11761 gttaacctta tcatctggca aaggtaagtt acgtctatcc caaacgtctt tatcccactc
    11821 ctctccatca aaataaaagt tctcataaaa ctccataaat tgttttagat tagtaacata
    11881 gtaggaatta gcttttacag gtgttttttc ttgattagca gtaatcttat aattagtagt
    11941 ggtaattcta acaccccgtt ttgtcaaata agttctatac tccgtcattg ctttttcaat
    12001 aggaacttca gtaattgaag taatgctagg atactttaaa tctaagaaat ctaacatttt
    12061 attaattact gttccttttc taatccagac agtttttgca ttccatattc cattgtttaa
    12121 atggtaaaaa taaaaatatt tcaattctgt tcttaaccac agatttttaa cacgttcaaa
    12181 acgaacccaa cgattcctta aagcaggatt cttacttaat tctatggcag aaggatgtgg
    12241 acattttctt atatcccaac tattattagc ccaaaacccc tgcatttctt cattcattac
    12301 agctattttt ttgctaatct cactctgact aataattttc cttttactag aagcattcat
    12361 ttcttatgct cctttctctc gaggtattta ttaaactcat ttttcatatc ctgatctgaa
    12421 agatgaacat aggtatttaa cgttgtctga acatgtgcgt gacctaatct cttttgaacg
    12481 aacgcaacat cccatccttc cctaattagc tgcgttgcgt gagtgtggcg aagcatatgt
    12541 gatgtaaatt ctattccagt ccttttaact attcttctaa ctagatcaag aacactttgg
    12601 tactttagtg gtttcccaaa atagccttct tttaaggaaa taaaaacata atcatgctcc
    12661 aattcctcac tatactcata tatcaagtaa tctgtataaa gtgacataag ttctttactc
    12721 acatgtattg ttctttcctt ccttaattta atataagctt cattaacatt aacatctcta
    12781 ggtgttaaat ggatttgatt gtcccaagtg acaatatctt caagcctaag cgataacact
    12841 tcaccgattc ttaaaccacc ctcatacata agcattaaaa ttaatttatc tcttttcgta
    12901 tgacaagcat caataatttg cttaacttcc tctgatctca atgttcttat ctgtttcttt
    12961 ttaaccctta actttaagac attcttttgg tatctaccct tattaacatg atgtaaaaat
    13021 cctttgaaat ttcttccctt tgcttgttta aatacatcaa ttgatttaaa ttctcctaat
    13081 ctacttaaat aatcaagaaa actcataact acatttaaaa ttgtattcac tgtcg
//
LOCUS       DANJLF010000264         8061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.099, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000264 DANJLF010000000
VERSION     DANJLF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            128..1564
                     /locus_tag="PWT25_003050"
     CDS             128..1564
                     /locus_tag="PWT25_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1023032.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="PWT25_003051"
     CDS             1753..4200
                     /locus_tag="PWT25_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL1023033.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="PWT25_003052"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="PWT25_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL1023034.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="PWT25_003053"
     CDS             complement(5690..6793)
                     /locus_tag="PWT25_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1023035.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="PWT25_003054"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="PWT25_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL1023036.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DANJLF010000265         2862 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.161, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000265 DANJLF010000000
VERSION     DANJLF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            complement(40..1026)
                     /locus_tag="PWT25_003055"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="PWT25_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="PWT25_003056"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="PWT25_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL1023037.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..2828
                     /locus_tag="PWT25_003057"
                     /pseudo
     CDS             1851..2828
                     /locus_tag="PWT25_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
BASE COUNT          889 a          570 c          575 g          828 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agtggttctg ttgcaaagtt ttaaataaag aataaaatcc ct
//
LOCUS       DANJLF010000266         2520 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.166, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000266 DANJLF010000000
VERSION     DANJLF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            41..229
                     /locus_tag="PWT25_003058"
                     /pseudo
     CDS             41..229
                     /locus_tag="PWT25_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
     gene            444..1412
                     /locus_tag="PWT25_003059"
     CDS             444..1412
                     /locus_tag="PWT25_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL1023038.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1405..2436
                     /gene="vanA"
                     /locus_tag="PWT25_003060"
     CDS             1405..2436
                     /gene="vanA"
                     /locus_tag="PWT25_003060"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL1023039.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
BASE COUNT          746 a          470 c          619 g          685 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacct gaaaagttct ataggctgga
       61 caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg caaaagaaat
      121 tattgttcag catggagggc agatttacgc ggaaagcaat gataactata cgacgtttag
      181 ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag agatgtatat
      241 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
      301 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      361 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      421 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      481 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      541 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      601 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      661 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      721 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      781 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      841 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      901 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      961 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
     1021 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
     1081 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
     1141 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
     1201 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1261 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1321 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1381 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1441 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1501 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1561 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1621 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1681 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1741 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1801 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1861 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1921 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1981 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     2041 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg cgaggtggac
     2101 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     2161 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     2221 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2281 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2341 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2401 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2461 ttttagatga aatagtacac ggggttctgt tgcaaagttt taaatctact atcaaataag
//
LOCUS       DANJLF010000267         1504 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.188, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000267 DANJLF010000000
VERSION     DANJLF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            262..345
                     /locus_tag="PWT25_003061"
                     /pseudo
     CDS             262..345
                     /locus_tag="PWT25_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="PWT25_003062"
     CDS             394..477
                     /locus_tag="PWT25_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDL1023040.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="PWT25_003063"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="PWT25_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL1023041.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DANJLF010000268         1350 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.199, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000268 DANJLF010000000
VERSION     DANJLF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            47..298
                     /locus_tag="PWT25_003064"
                     /pseudo
     CDS             47..298
                     /locus_tag="PWT25_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="PWT25_003065"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="PWT25_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL1023042.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DANJLF010000269          853 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.227, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000269 DANJLF010000000
VERSION     DANJLF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..782
                     /gene="aph(2'')-I"
                     /locus_tag="PWT25_003066"
     CDS             <1..782
                     /gene="aph(2'')-I"
                     /locus_tag="PWT25_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDL1023043.1"
                     /translation="NEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSY
                     ISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTI
                     DNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHL
                     LLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAK
                     EYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          371 a           61 c          135 g          286 t
ORIGIN      
        1 ataatgaata catttttaaa acaaaattta gtactaataa gaaaaaaggt tatgcaaaag
       61 aaaaagcaat atataatttt ttaaatacaa atttagaaac taatgtaaaa attcctaata
      121 ttgaatattc gtatattagt gatgaattat ctatactagg ttataaagaa attaaaggaa
      181 cttttttaac accagaaatt tattctacta tgtcagaaga agaacaaaat ttgttaaaac
      241 gagatattgc cagtttttta agacaaatgc acggtttaga ttatacagat attagtgaat
      301 gtactattga taataaacaa aatgtattag aagagtatat attgttgcgt gaaactattt
      361 ataatgattt aactgatata gaaaaagatt atatagaaag ttttatggaa agactaaatg
      421 caacaacagt ttttgagggt aaaaagtgtt tatgccataa tgattttagt tgtaatcatc
      481 tattgttaga tggcaataat agattaactg gaataattga ttttggagat tctggaatta
      541 tagatgaata ttgtgatttt atatacttac ttgaagatag tgaagaagaa ataggaacaa
      601 attttggaga agatatatta agaatgtatg gaaatataga tattgagaaa gcaaaagaat
      661 atcaagatat agttgaagaa tattatccta ttgaaactat tgtttatgga attaaaaata
      721 ttaaacagga atttatcgaa aatggtagaa aagaaattta taaaaggact tataaagatt
      781 gattatataa tatatgaaaa gctattataa aagacggttc tgttgcaaag ttttaaatct
      841 actatcaaat aag
//
LOCUS       DANJLF010000270          579 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538560-rid17231343.guided.244, whole genome shotgun
            sequence.
ACCESSION   DANJLF010000270 DANJLF010000000
VERSION     DANJLF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538560
            Sequence Read Archive: ERR10688123
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637868.1
            Genome Coverage       :: 110x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:57:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 27 of 80
            Pseudo Genes (incomplete)         :: 40 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 6 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538560-rid17231343.guided.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-12-16"
     gene            <1..>579
                     /locus_tag="PWT25_003067"
     CDS             <1..>579
                     /locus_tag="PWT25_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1023044.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEY"
BASE COUNT          232 a           54 c          105 g          188 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaatac
//
